Search

The effectiveness of low-intensity red light on myofascial trigger points in skeletal muscle of mature rats was evaluated by electron microscopy and high-resolution respirometry. The revealed changes in mitochondrial ultrastructure and activity of the respiratory chain enzymes indicate the development of hypoxia in the simulation area. Under the influence of low-intensity red light on myofascial trigger points, a decrease in the number of destructively altered muscle fibers and stimulation of mitochondrial respiration were found. These findings indicate intracellular regeneration and the stimulating effect of low-intensity red light on plastic processes., (© 2023. Springer Science+Business Media, LLC, part of Springer Nature.)

The aim of the study was to define the role of brain-derived and glial cell line-derived neurotrophic factors (BDNF and GDNF) in realization of compensative and adaptive mechanisms of a neonatal organism to hypoxia., Materials and Methods: The experiments in vivo have been carried out on pregnant C57BL/6 mice (n=36). Chronic hypobaric hypoxia has been modeled in different pregnancy trimesters. On gestation days E19-20, concentration of BDNF and GDNF in the blood of the pregnant females was determined by enzyme immunoassay. Further, the number of neonatal mice, their weight and body length parameters have been assessed.Parturient mothers (n=88) and their newborn babies followed up at the Clinic of Obstetrics and Gynecology of Pavlov University took part in the clinical investigations. Concentration of BDNF, GDNF, neuron-specific enolase (NSE), and hypoxia-inducible factor (HIF-1β) in the fetal cord blood has been determined by ELISA. The obtained data were retrospectively compared with cardiotocography, dopplerometry, presence of meconium-stained amniotic fluid and the neonate state at birth, assessment according to the Apgar score, and the course of adaptation period., Results: Chronic hypobaric hypoxia in pregnant mice in trimester I and II resulted in the significant decrease of BDNF and GDNF level, decrease in the number of embryos, and in significant changes in weight/height characteristics of the newborn pups.According to the clinical observations, an increased expression of the neurotrophic factors BDNF, GDNF provides protection to a neonate even if hypoxia factors are present and realized. A low content of BDNF and GDNF was observed in the group of infants with a high risk of developing unfavorable hypoxic damaging effects., Conclusion: The protective role of BDNF and GDNF in the regulation of fetal homeostasis in chronic hypoxia has been established experimentally and clinically., Competing Interests: Conflicts of interest. The authors have no conflicts of interest to declare.

The level of 8-OH-2-desoxiguanosin in blood serum of patients with severe chronic dermatitis was analyzed. It is demonstrated that in comparison with healthy patients in examined group of patients this indicator is increased mainly in patients with atopic dermatitis (up to 62%) and least of all with psoriasis (up to 25%) and bullous dermatitis (up to 18%). The statistically reliable differences of this indicator from control values under endogenic intoxication and different degree of severity of disease are determined. The detection of level of 8-OH-2-desoxiguanosin in blood serum is an informative technique of analysis to increase quality of laboratory monitoring of oxidizing stress.

We compared efficacy of combined treatment of males with anogenital warts given intramuscular cycloferon or cycloferon electrophoresis. Patients with manifest HPV infection (n=107) were divided into two groups. Group 1 (n=72) received intramuscular cycloferon (2 ml 12.5% solution each other day for 10 days), group 2 (n=35) received cycloferon electrophoresis on genital region (2 ml 12.5% solution daily in 1-2 days, 5 injections). Measurements were made of local cytokines in urethral secretion, IFN-alpha, IFN-gamma, lactoferrin, sexual hormones (estradiol and testosterone) in blood serum before and after treatment. Focal immunophysiotherapy with cycloferon in combined treatment of males with anogenital warts vs intramuscular cycloferon treatment reduces recurrence rate 2 times, has no negative effect on serum level of sexual hormones, a course dose and duration of immunotherapy decreased 2-fold, is well tolerated, is not painful. Cycloferon electrophoresis has a more potent effect on improvement of local immunity. Thus, local immunophysiotherapy with cycloferon can be a method of choice in the treatment of manifest HPV infection in males.