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21 results on '"Shattuck, Donna"'

4. Predisposition locus for major depression at chromosome 12q22-12q23.2

Author

Abkevich, Victor, Camp, Nicola J., Hensel, Charles H., Neff, Chris D., Russell, Deanna L., Hughes, Dana C., Plenk, Agnes M., Lowry, Michael R., Richards, R. Lynn, Carter, Catherine, Frech, Georges C., Stone, Steven, Rowe, Kerry, Chau, Chi Ai, Cortado, Kathleen, Hunt, Angelene, Luce, Karanina, O'Neil, Gayanne, Poarch, Jeff, Potter, Jennifer, Poulsen, Gregg H., Saxton, Heidi, Bernat-Sestak, Michelle, Thompson, Victor M., Gutin, Alexander, Skolnick, Mark H., Shattuck, Donna, and Cannon-Albright, Lisa

Subjects
Depression, Mental -- Research, Human genetics -- Research, Biological sciences
Published
2003

5. Linkage of body mass index to chromosome 20 in Utah pedigrees

Author

Hunt, Steven C., Abkevich, Victor, Hensel, Charles H., Gutin, Alexander, Neff, Chris D., Russell, Deanna L., Tran, Thanh, Hong, Xiankang, Jammulapati, Srikanth, Riley, Robyn, Weaver-Feldhaus, Jane, Macalma, Tess, Richards, Maria M., Gress, Richard, Francis, Mike, Thomas, Alun, Frech, Georges C., Adams, Ted D., Shattuck, Donna, and Stone, Steven

Published
2001
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6. A major predisposition locus for severe obesity, at 4p15-p14

Author

Stone, Steven, Abkevich, Victor, Hunt, Steven C., Gutin, Alexander, Russell, Deanna L., Neff, Chris D., Riley, Robyn, Frech, Georges C., Hensel, Charles H., Jammulapati, Srikanth, Potter, Jennifer, Sexton, David, Tran, Thanh, Gibbs, Drew, Iliev, Diana, Gress, Richard, Bloomquist, Brian, Amatruda, John, Rae, Peter M.M., Adams, Ted D., Skolnick, Mark H., and Shattuck, Donna

Subjects
Genetic research -- Analysis, Obesity -- Genetic aspects, Biological sciences
Published
2002

9. The human-bacterial pathogen protein interaction networks of Bacillus anthracis, Francisella tularensis, and Yersinia pesti

Author

Dye, Matthew D., Neff, Chris, Dufford, Max, Rivera, Corban G., Shattuck, Donna, Bassaganya-Riera, Josep, Murali, T. M., Sobral, Bruno, Dye, Matthew D., Neff, Chris, Dufford, Max, Rivera, Corban G., Shattuck, Donna, Bassaganya-Riera, Josep, Murali, T. M., and Sobral, Bruno

Abstract

Background: Bacillus anthracis, Francisella tularensis, and Yersinia pestis are bacterial pathogens that can cause anthrax, lethal acute pneumonic disease, and bubonic plague, respectively, and are listed as NIAID Category A priority pathogens for possible use as biological weapons. However, the interactions between human proteins and proteins in these bacteria remain poorly characterized leading to an incomplete understanding of their pathogenesis and mechanisms of immune evasion. Methodology: In this study, we used a high-throughput yeast two-hybrid assay to identify physical interactions between human proteins and proteins from each of these three pathogens. From more than 250,000 screens performed, we identified 3,073 human-B. anthracis, 1,383 human-F. tularensis, and 4,059 human-Y. pestis protein-protein interactions including interactions involving 304 B. anthracis, 52 F. tularensis, and 330 Y. pestis proteins that are uncharacterized. Computational analysis revealed that pathogen proteins preferentially interact with human proteins that are hubs and bottlenecks in the human PPI network. In addition, we computed modules of human-pathogen PPIs that are conserved amongst the three networks. Functionally, such conserved modules reveal commonalities between how the different pathogens interact with crucial host pathways involved in inflammation and immunity. Significance: These data constitute the first extensive protein interaction networks constructed for bacterial pathogens and their human hosts. This study provides novel insights into host-pathogen interactions.

Published
2010
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14. Proteomic screening of variola virus reveals a unique NF-κB inhibitor that is highly conserved among pathogenic orthopoxviruses

Author

Mohamed, Mohamed R., primary, Rahman, Masmudur M., additional, Lanchbury, Jerry S., additional, Shattuck, Donna, additional, Neff, Chris, additional, Dufford, Max, additional, van Buuren, Nick, additional, Fagan, Katharine, additional, Barry, Michele, additional, Smith, Scott, additional, Damon, Inger, additional, and McFadden, Grant, additional

Published
2009
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17. Genome‐wide linkage analyses of extended Utah pedigrees identifies loci that influence recurrent, early‐onset major depression and anxiety disorders

Author

Camp, Nicola J., primary, Lowry, Michael R., additional, Richards, R. Lynn, additional, Plenk, Agnes M., additional, Carter, Catherine, additional, Hensel, Charles H., additional, Abkevich, Victor, additional, Skolnick, Mark H., additional, Shattuck, Donna, additional, Rowe, Kerry G., additional, Hughes, Dana C., additional, and Cannon‐Albright, Lisa A., additional

Published
2005
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18. Characterization of TRZ1, a yeast homolog of the human candidate prostate cancer susceptibility gene ELAC2 encoding tRNase Z

Author

Chen Yuan, Davenport Christina, Beck Audrey, Chen Yang, Shattuck Donna, and Tavtigian Sean V

Subjects
Male, Saccharomyces cerevisiae Proteins, lcsh:QH426-470, RNA Splicing, Amino Acid Motifs, Molecular Sequence Data, DNA, Recombinant, Saccharomyces cerevisiae, RNA, Transfer, Sequence Analysis, Protein, Endoribonucleases, Humans, Amino Acid Sequence, RNA, Messenger, lcsh:QH573-671, RNA Processing, Post-Transcriptional, Sequence Homology, Amino Acid, lcsh:Cytology, Genetic Complementation Test, Temperature, Prostatic Neoplasms, Mitochondria, Neoplasm Proteins, Gene Expression Regulation, Neoplastic, lcsh:Genetics, RNA, Ribosomal, Mutation, Research Article
Abstract

Background In humans, mutation of ELAC2 is associated with an increased risk of prostate cancer. ELAC2 has been shown to have tRNase Z activity and is associated with the γ-tubulin complex. Results In this work, we show that the yeast homolog of ELAC2, encoded by TRZ1 (tRNase Z 1), is involved genetically in RNA processing. The temperature sensitivity of a trz1 mutant can be rescued by multiple copies of REX2, which encodes a protein with RNA 3' processing activity, suggesting a role of Trz1p in RNA processing in vivo. Trz1p has two putative nucleotide triphosphate-binding motifs (P-loop) and a conserved histidine motif. The histidine motif and the putative nucleotide binding motif at the C-domain are important for Trz1p function because mutant proteins bearing changes to the critical residues in these motifs are unable to rescue deletion of TRZ1. The growth defect exhibited by trz1 yeast is not complemented by the heterologous ELAC2, suggesting that Trz1p may have additional functions in yeast. Conclusion Our results provide genetic evidence that prostate cancer susceptibility gene ELAC2 may be involved in RNA processing, especially rRNA processing and mitochondrial function.

Published
2005

19. Characterization of TRZ1, a yeast homolog of the human candidate prostate cancer susceptibility gene ELAC2 encoding tRNase Z.

Author

Yang Chen, Beck, Audrey, Davenport, Christina, Yuan Chen, Shattuck, Donna, and Tavtigian, Sean V.

Subjects
PROSTATE cancer, CANCER susceptibility, RNA, TUBULINS, CANCER, NUCLEOTIDES
Abstract

Background: In humans, mutation of ELAC2 is associated with an increased risk of prostate cancer. ELAC2 has been shown to have tRNase Z activity and is associated with the γ-tubulin complex. Results: In this work, we show that the yeast homolog of ELAC2, encoded by TRZ1 (tRNase Z 1), is involved genetically in RNA processing. The temperature sensitivity of a trz1 mutant can be rescued by multiple copies of REX2, which encodes a protein with RNA 3' processing activity, suggesting a role of Trz1p in RNA processing in vivo. Trz1p has two putative nucleotide triphosphate-binding motifs (P-loop) and a conserved histidine motif. The histidine motif and the putative nucleotide binding motif at the C-domain are important for Trz1p function because mutant proteins bearing changes to the critical residues in these motifs are unable to rescue deletion of TRZ1. The growth defect exhibited by trz1 yeast is not complemented by the heterologous ELAC2, suggesting that Trz1p may have additional functions in yeast. Conclusion: Our results provide genetic evidence that prostate cancer susceptibility gene ELAC2 may be involved in RNA processing, especially rRNA processing and mitochondrial function. [ABSTRACT FROM AUTHOR]

Published
2005
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20. Evidence for epistasis between SLC6A4 and a chromosome 4 gene as risk factors in major depression

Author

Neff, Chris D., Abkevich, Victor, Potter, Jennifer, Riley, Robin, Shattuck, Donna, and Katz, David A.

Abstract

Linkage analysis on Utah pedigrees with strong family histories of major depression including only cases with the SLC6A4HTTLPR short allele revealed a linkage peak on chromosome 4 maximum HLOD  3.5. This evidence suggests epistasis between SLC6A4and an unknown gene as risk factors for major depression. © 2009 WileyLiss, Inc.

Published
2010
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21. Analysis of vaccinia virus-host protein-protein interactions: validations of yeast two-hybrid screenings.

Author

Zhang L, Villa NY, Rahman MM, Smallwood S, Shattuck D, Neff C, Dufford M, Lanchbury JS, Labaer J, and McFadden G

Subjects
Humans, Open Reading Frames, Polymerase Chain Reaction, Protein Interaction Mapping methods, Proteomics methods, Recombinant Fusion Proteins metabolism, Reproducibility of Results, Host-Pathogen Interactions physiology, Two-Hybrid System Techniques, Vaccinia metabolism, Viral Proteins metabolism
Abstract

Vaccinia virus, a large double-stranded DNA virus, is the prototype of the Orthopoxvirus genus, which includes several pathogenic poxviruses of humans, such as monkeypox virus and variola virus. Here, we report a comprehensive yeast two-hybrid (Y2H) screening for the protein-protein interactions between vaccinia and human proteins. A total of 109 novel vaccinia-human protein interactions were detected among 33 viral proteins. To validate subsets of those interactions, we constructed an ORFeome library of vaccinia virus strain WR using the Gateway plasmid cloning system. By co-expressing selected vaccinia and host proteins in a variety of expression systems, we found that at least 17 of the Y2H hits identified between vaccinia and human proteins can be verified by independent methods using GST pull-down assays, representing a 63% validation rate for the Y2H hits examined (17/27). Because the cloned ORFs are conveniently transferable from the entry vectors to various destination expression vectors, the vaccinia ORFeome library will be a useful resource for future high-throughput functional proteomic experiments.

Published
2009
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