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Human microbial alterations are associated with environmental stress, nutritional, genetic and triggering de-novo variations. Nevertheless, human gut microbiome at extreme altitude (>5800 m) remains unexplored. We aimed to demonstrate the microbial predominance in individuals with same ethnicity and dietary pattern at extreme altitude with unique challenges like cold, hypoxia, radiation etc. Different analysis pipelines were used for fecal whole genome sequencing at 210m, 3500m, 4420m and 5805m, and 16s rRNA V3-V4 regions amplification sequencing of 19 individuals belonging to the same ethnicity and dietary pattern, for presence of taxonomy & functional potential and confirming the prediction upto the strain level within the same cohort. Principal component analysis, revealed distinct microbiome changes at different altitudes, with varied and higher Bacteroides and Prevotella ratio. There was predominance of genus Prevotella at altitudes 4420m & 5805m than at 210m & 3500m. Appearance of species Prevotella copri strain 61740 was increasing significantly at extreme altitudes, whereas co-occurrence of other bacterial strains had different pattern than Prevotella. The extensive strain level analysis indicated alteration in the metabolic pathways. This study under stressful and hypoxic environment of extreme altitudes, associated microbial variation with altered metabolic pathways, reveals influence of extreme environment on human gut microbiota with predominance of Prevotella.

Mastitis has well-recognized harmful effects on dairy farm profitability. Furthermore, mastitis impairs the milk component synthesizing ability of secretary tissues. Various therapies are available for the treatment of clinical mastitis. Meloxicam exhibits preferential binding to Cyclooxygenase-2 (COX-2) receptor and consequently generates fewer negative gastrointestinal side effects than nonspecific COX inhibitors such as flunixin meglumine and ketoprofen. Toward this end, research efforts directed at understanding the use of meloxicam alone and in combination with other antibiotics to improve milk quality and production. Therefore, in this review, we have highlighted the mechanism, biopharmaceutical challenges, and merits of meloxicam usage in dairy cattle mastitis. In addition, we also presented the integration of artificial neural network

Background: Threatened abortion in first trimester is a risk factor for complications in the latter half of pregnancy. The present study was undertaken to identify the pregnancy outcome in women with threatened abortion in the first trimester of pregnancy in a tertiary hospital. Methods: A retrospective-prospective observational study was done on 100 pregnant women with a history of threatened abortion in the first trimester. Maternal outcome in the form of pregnancy loss, APH/PPH, Preeclampsia/eclampsia, PROM/PPROM, term delivery and retained placenta were studies. Perinatal outcome in the form of preterm delivery, Low Birth Weight, IUGR and IUFD were studied. Analysis of the data was done using SPSS version 17. Results: Threatened abortion in first trimester was found to be associated with increased risks of LBW, preterm births, PPROM, and PIH. Out of 100 patients, preterm births and LBW babies were found in 21% and 13% of patients respectively. 15% of the cases developed PIH, out of which gestational hypertension and pre-eclampsia were 8% and 6% respectively. Conclusions: Pregnant women with first trimester threatened abortion are at increased risk for spontaneous loss and adverse pregnancy outcomes. Knowledge of these risks may help the obstetricians to manage these cases vigorously in the antepartum period and do timely interventions as needed for a healthy mother and baby.

Background: Few women during their pregnancy, labour and postnatal period require critical care related to the pregnancy itself, aggravation of a pre-existing illness and complications of the delivery. Pregnant patients account for a small number of ICU admission - 2-10% two main indications for admission are hypertensive disorders (17.2%-46%) and massive haemorrhage (10%-32.8%). The primary objective of the present study was to review the characteristics of the obstetric patients admitted to our ICU over a 2-year period.Methods: It was a prospective study conducted over 50 patients in high dependency and intensive care unit at Alam hospital over a period of 2 years (October 2014 to October 2016).Results: During the study period a total of 50 obstetric patients were transferred to the intensive care unit (ICU). Antenatal care played significant role in the obstetric outcome. 84% of patients transferred to the ICU during the study period had inadequate or no antenatal care, while 8% were booked in their pregnancy and had adequate antenatal care. ICU interventions included mechanical ventilation used in 20(40%) patients, blood and blood product transfusion in 35(70%) patients’ inotropes in 20 patients (40%) antihypertensive therapy in 20 patients (40%), arterial embolization in 2(4%) patients. Maternal mortality was seen in 10 patients (20%).Conclusions: There is a need for training in emergency obstetrics so that the complication can be managed right at the time of occurrence.

Background: The worldwide incidence of GTB is approximately 5- 10% in infertile women. It varies from as low as 0.69% in some developed countries to as high as 19% in India. It is diagnosed by culture of the tubercle bacillus from tissue sampled from the genital tract is the yardstick for diagnosis and remains the gold standard.Methods: A prospective study was carried out between January 2012 and January 2015 on 100 women presenting with infertility.Results: A total (27%) women were diagnosed as genital tuberculosis by combination of hystero laparoscopic findings, histopathological and endometrial DNA-PCR technique confirmation. Of these (40.62%) had secondary infertility and remaining (59.38%) had primary infertility. (15.62%) were previously diagnosed with pulmonary or extra pulmonary tuberculosis and had completed a full course of Anti-tubercular treatment as per WHO CAT 1 regime.Conclusions: Therefore, in countries where TB is endemic, early and aggressive strategies should be pursued to diagnose and treat TB.

Background: The aim of this study is to determine the effectiveness of McIndoe Vaginoplasty in the creation of a neovagina for patients with vaginal agenesis.Methods: this is a retrospective study of 50 cases of vaginal agenesis, who underwent McIndoe Vaginoplasty over a period of 8 years (2004-2012) by using skin graft in 25 cases and amnion as graft in the remaining.Results: McIndoe Vaginoplasty was performed successfully in all 50 patients and post-surgical result was acceptable to the patient psychologically, sexually and aesthetically.Conclusions: McIndoe Vaginoplasty is a safe and effective procedure for providing a satisfactory and functional vagina in patients with MRKH syndrome.

Background: Uterine rupture is a grave condition which is almost fatal for fetus. The most common risk factor for the uterine rupture is previous uterine surgery. Other major factors are obstructed labor, multiparity, use of uterotonic drugs, placenta percreta and rarely intrauterine manipulations such as internal podalic version and breech extraction.Methods: This study was conducted over a period from March 2014 to September 2015, in the Department of Gynecology and Obstetrics, RIMS Ranchi, Jharkhand. All cases of rupture uterus, who were either admitted with or who developed this complication in the hospital, were included in the study.Results: There were 80 cases of rupture uterus out of 10474 deliveries. The incidence of rupture uterus was more (97.5%) in cases who had no previous antenatal checkup at all. Most of cases of previous caesarean section scar rupture during labour was lower segment caesarean section scar (94.11%). The most common causes of traumatic rupture were injudicious use of oxytocics (75%). Majority of cases of rupture uterus were of complete type (96.25%). The most frequent site of rupture was in the anterior wall of lower segment in 68.75% of cases. The maternal mortality rate cases of rupture uterus were 3.75%.Conclusions: Proper antenatal care and updated training courses of health care providers should be stressed to prevent this catastrophic but avoidable complication.

Background: Maternal near miss is said to have occurred when women presented with life threatening complication during pregnancy, child birth and within 42 days after delivery, but survive by chance or good institutional care. For identifying near-miss cases five-factor scoring system was used. In 2009 WHO working group has standardized the criteria for selecting these cases.Methods: The study was conducted in the Department of Obstetrics and Gynecology at RIMS, Ranchi, Jharkhand, India, which is a tertiary care centre. For each case of near miss, data were collected on demographic characteristics including gestational age at the time of sustaining the near-miss morbidity, nature of obstetric complications, presence of organ-system dysfunction/failure, ICU admission and timing of near-miss event with respect to admission.Results: During the twenty-four months of the study period, 20000 deliveries at the institution and 480 women were identified as near-miss obstetrical cases by five factor scoring system. The prevalence of near-miss case in this study was 2.4%. Near-miss per 1000 delivery was 24%. Maternal death to near miss ratio was 1:7.2. The leading causes of maternal near miss were hemorrhage (42.5%) and hypertensive disorder of pregnancy (23.5%) The morbidity was high in unbooked cases.Conclusions: Maternal near miss is good alternative indicator of health care system.

Quorum Sensing (QS) is a natural phenomenon of communication circuits to regulate a diverse array of physiological activities. QS regulates microbial diversity and ecological balance through various process, i.e., symbiosis, motility, sporulation, virulence, biofilm formation, competence, conjugation, and antibiotic production. There are numerous bacteria, fungi, and plants that have components of a QS system to control a specific phenotype of an organism and to shape the microbial community in the rhizophere. Bacterial intercellular communication is based on the detection of diffusible signal molecules and their transduction mechanisms which convert the information into appropriate molecular–cellular responses. Recent advances in studies of QS systems have brought the understanding that biocommunication and community behavior are crucial parameters for the successful interactions in a mycorrhizosphere. We are only at the beginning to understand how plants control microbial communities in the rhizophere. Interspecies and cross-kingdom communication is implicated in successful symbiotic interactions of a variety of bacteria with fungi and plant systems.