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31 results on '"Sharabi, Mirit"'

5. Nonlinear elasticity of the extracellular matrix fibers facilitates efficient inter-cellular mechanical communication

Author

Sopher, Ran S, Tokash, Hanan, Natan, Sari, Sharabi, Mirit, Shelah, Ortal, Tchaicheeyan, Oren, and Lesman, Ayelet

Subjects
Physics - Biological Physics
Abstract

Biological cells embedded in fibrous matrices have been observed to form inter-cellular bands of dense and aligned fibers, through which they mechanically interact over long distances. Such matrix-mediated cellular interactions have been shown to regulate a variety of biological processes. The current study was aimed at exploring the effects of elastic nonlinearity of the fibers contained in the extracellular matrix (ECM) on the transmission of mechanical loads between contracting cells. Based on our biological experiments, we developed a finite-element model of two contracting cells embedded within a fibrous network. The individual fibers were modeled as showing either linear elasticity, compression-microbuckling, tension-stiffening or both of the latter. Compression-buckling resulted in smaller loads occurring in the ECM, but these were more directed toward the neighboring cell. The latter decreased with increasing cell-to-cell distance; when cells were >15 cell-diameters apart, no such inter-cellular interaction was observed. Tension-stiffening further contributed to directing the loads toward the neighboring cell, though to a smaller extent. The contraction of two neighboring cells resulted in mutual attraction forces, which were considerably increased by tension-stiffening, and decayed with increasing cell-to-cell distances. Nonlinear elasticity contributed also to the onset of force polarity on the cell boundary. The density and alignment of the fibers within the inter-cellular band were considerably greater when fibers buckled under compression, with tension-stiffening further contributing to this structural remodeling. Our model demonstrates the contribution of nonlinear elasticity of biological gels to directionality and efficiency of mechanical-signal transfer between distant cells.

Published
2018
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13. Contributors

Author

Bassani, Tito, primary, Brayda-Bruno, Marco, additional, Casaroli, Gloria, additional, Costa, Francesco, additional, Galbusera, Fabio, additional, Graf, Nicolas, additional, Haj-Ali, Rami, additional, Ismael Aguirre, Maryem-Fama, additional, Jonas, René, additional, Kienle, Annette, additional, La Barbera, Luigi, additional, Liebsch, Christian, additional, Lindtner, Richard A., additional, Luca, Andrea, additional, Malandrino, Andrea, additional, Niemeyer, Frank, additional, Ottardi, Claudia, additional, Schlager, Benedikt, additional, Schmoelz, Werner, additional, Sconfienza, Luca M., additional, Sharabi, Mirit, additional, Stucovitz, Elena, additional, Toumanidou, Themis, additional, Villa, Tomaso, additional, Vitale, Jacopo, additional, Volkheimer, David, additional, Wade, Kelly, additional, and Wilke, Hans-Joachim, additional

Published
2018
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26. Erratum: “The Mechanical Role of the Radial Fibers Network Within the Annulus Fibrosus of the Lumbar Intervertebral Disc: A Finite Elements Study” [ASME J. Biomech. Eng., 2018, 141(2), p. 021006: DOI: 10.1115/1.4041769]

Author

Sharabi, Mirit, primary, Levi-Sasson, Aviad, additional, Wolfson, Roza, additional, Wade, Kelly R., additional, Galbusera, Fabio, additional, Benayahu, Dafna, additional, Wilke, Hans-Joachim, additional, and Haj-Ali, Rami, additional

Published
2019
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31. The Mechanical Role of the Radial Fiber Network Within the Annulus Fibrosus of the Lumbar Intervertebral Disc: A Finite Elements Study.

Author

Sharabi M, Levi-Sasson A, Wolfson R, Wade KR, Galbusera F, Benayahu D, Wilke HJ, and Haj-Ali R

Abstract

The annulus fibrosus (AF) of the intervertebral disc (IVD) consists of a set of concentric layers composed of a primary circumferential collagen fibers arranged in an alternating oblique orientation. Moreover, there exists an additional secondary set of radial translamellar collagen fibers which connects the concentric layers, creating an interconnected fiber network. The aim of this study was to investigate the mechanical role of the radial fiber network. Toward that goal, a three-dimensional (3D) finite element model of the L3-L4 spinal segment was generated and calibrated to axial compression and pure moment loading. The AF model explicitly recognizes the two heterogeneous networks of fibers. The presence of radial fibers demonstrated a pronounced effect on the local disc responses under lateral bending, flexion, and extension modes. In these modes, the radial fibers were in a tensile state in the disc region that subjected to compression. In addition, the circumferential fibers, on the opposite side of the IVD, were also under tension. The local stress in the matrix was decreased in up to 9% in the radial fibers presence. This implies an active fiber network acting collectively to reduce the stresses and strains in the AF lamellae. Moreover, a reduction of 26.6% in the matrix sideways expansion was seen in the presence of the radial fibers near the neutral bending axis of the disc. The proposed biomechanical model provided a new insight into the mechanical role of the radial collagen fibers in the AF structure. This model can assist in the design of future IVD substitutes., (Copyright © 2019 by ASME.)

Published
2019
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