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BackgroundSepsis is the body’s extreme response to an infection leading to organ dysfunction. Sepsis-related acquired weakness (SAW), a critical illness closely related to metabolic disorders, is characterized by generalized sepsis-induced skeletal muscle weakness, mainly manifesting as symmetrical atrophy of respiratory and limb muscles. Muscle accounts for 40% of the body’s total mass and is one of the major sites of glucose and energy absorption. Diet affects skeletal muscle metabolism, which further impacts physiology and signaling pathways. The ketogenic diet (KD) is a high-fat, low-carbohydrate diet that has shown benefits in patients with a variety of neuromuscular disorders. Patients with SAW are in a hypermetabolic state and can consume approximately 1% of total body muscle mass in a day. Due to the decreased total body energy expenditure secondary to starvation, skeletal muscles enter a low metabolic state, with reduced gluconeogenesis and protein consumption and elevated levels of ketone bodies. The latest research suggests that KD may be a new strategy for SAW prevention and treatment, but its mechanism is still unclear.ObjectiveOur article aims to explore the effect and mechanism of KD on SAW. And we hope that our review will inspire further research on the KD and foster the exploration of novel strategies for combating SAW.MethodsSearch medical databases and related academic websites, using keywords such as “Sepsis-related acquired weakness,” “ketogenic diet,” and “skeletal muscle,” and select representative literature. Using the method of induction and summary, analyze the effect and mechanism of KD on SAW.ResultsCompared with early nutrition, KD has a more protective effect on SAW, but its mechanism is complex. Firstly, KD can alter energy metabolism substrates to affect SAW’s energy metabolism; Secondly, KD can directly act as a signaling molecule to improve mitochondrial function in skeletal muscle and stimulate skeletal muscle regeneration signaling molecules; Thirdly, KD can affect the gut microbiota to exert anti-inflammatory effects, enhance immunity, and thus protect SAW.ConclusionKD has a protective effect on SAW, which includes improving energy metabolism, stimulating muscle regeneration signals, optimizing gut microbiota composition, and reducing inflammation and oxidative stress.

BackgroundPulmonary rehabilitation (PR) has been shown to effectively support postsurgical recovery in patients with lung cancer (LC) at various stages. While digital PR programs offer a potential solution to traditional challenges, such as time and space constraints, their efficacy and feasibility for patients undergoing LC surgery remain unclear. ObjectiveThis systematic review aims to assess the feasibility and effectiveness of digital PR programs for individuals undergoing LC surgery. MethodsA systematic review was conducted, retrieving data from 6 English and 4 Chinese databases from their inception to January 1, 2024. References in related studies were also manually reviewed. The primary outcomes assessed were physical capacity, lung function, and the incidence of postoperative pulmonary complications (PPCs). The secondary outcomes were compliance, hospital stay, chest tube duration, anxiety, depression, and quality of life. Where applicable, recruitment and withdrawal rates were also evaluated. Meta-analysis and descriptive analysis were used to assess the outcomes. ResultsA total of 5 randomized controlled trials and 6 quasi-experimental studies (n=1063) were included, with 4 studies being included in the meta-analyses. Our meta-analyses revealed that digital PR reduced the decline in 6-minute walk distance (6-MWD) by an average of 15 m compared with routine PR programs from admission to discharge, demonstrating a clinically significant improvement in physical capacity (mean difference –15.00, 95% CI –25.65 to –4.34, P=.006). Additionally, digital PR was associated with a reduction (26/58, 45%) in the likelihood of PPCs (risk ratio 0.45, 95% CI 0.30-0.66, P

BackgroundAnimal models focusing on neuromuscular outcomes are crucial for understanding the mechanisms of intensive care unit-acquired weakness (ICU-AW) and exploring potential innovative prevention and treatment strategies.AimTo analyse and evaluate preclinical ICU-AW models.MethodsWe manually searched five English and four Chinese databases from 1 January 2002, to 1 February 2024, and reviewed related study references. Full-text publications describing animal models of muscle weakness and atrophy in critical illness were included. Detailed information about model types, animal species, sex, age, induction methods, outcome measures, drawbacks and strengths was extracted from each included study.ResultsA total of 3,451 citations were initially retrieved, with 84 studies included in the final analysis. The most frequently studied animal model included rodents (86.9%), 64.3% of which were male animals. ICU-AW animal models were mostly induced by comprehensive intensive care unit (ICU) interventions (38.1%) and sepsis (51.2%). Most studies focused on limb muscles (66.7%), diaphragm muscles (21.4%) or both (9.5%). Reported outcomes primarily included muscular pathological changes (83.3%), electrophysiological examinations of muscles (57.1%) and animal grip strength (16.6%). However, details such as animal age, mortality data, experimental design, randomisation, blinding, sample size and interventions for the experimental group and/or control group were inadequately reported.ConclusionMany preclinical models are used to study ICU-AW, but the reporting of methodological details is often incomplete. Although current ICU animal models can mimic the characteristics of human ICU-AW, there is no standard model. Future preclinical studies should develop a standard ICU-AW animal model to enhance reproducibility and improve scientific rigor in exploring the mechanisms and potential treatment of ICU-AW.

Abstract Background Incentive spirometry (IS) as a routine respiratory therapy during the perioperative period has been widely used in clinical practice. However, the impact of IS on patients with perioperative lung cancer remains controversial. This review aimed to evaluate the efficacy of IS in perioperative pulmonary rehabilitation for patients with lung cancer. Methods Cochrane Library, PubMed, Web of Science, Ovid, CINAHL, Chinese National Knowledge Infrastructure, Weipu, and Wanfang Databases were searched from inception to 30 November 2023. Only randomized controlled trials were included in this systematic review. The PRISMA checklist served as the guidance for conducting this review. The quality assessment of the included studies was assessed by the Cochrane risk-of-bias tool. The meta-analysis was carried out utilizing Review Manager 5.4. Furthermore, sensitivity analysis and subgroup analysis were also performed. Results Nine studies recruited 1209 patients met our inclusion criteria. IS combined with other respiratory therapy techniques was observed to reduce the incidence of postoperative pulmonary complications, enhance pulmonary function, curtail the length of hospital stay, and lower the Borg score. Nevertheless, no improvements were found in the six-minute walk distance or quality of life score. Conclusions Although IS demonstrates benefits as a component of comprehensive intervention measures for perioperative patients with lung cancer, it proves challenging to determine the precise impact of IS as a standalone component within the comprehensive intervention measures. Therefore, further researches are required to better understand the effectiveness of IS isolation and its interactions when integrated with additional respiratory therapies for these patients. Clinical trial registration PROSPERO, https://www.crd.york.ac.uk/prospero/ , registry number: CRD42022321044.

Abstract Background Bioconversion of plant biomass into biofuels and bio-products produces large amounts of lignin. The aromatic biopolymers need to be degraded before being converted into value-added bio-products. Microbes can be environment-friendly and efficiently degrade lignin. Compared to fungi, bacteria have some advantages in lignin degradation, including broad tolerance to pH, temperature, and oxygen and the toolkit for genetic manipulation. Results Our previous study isolated a novel ligninolytic bacterial strain Erwinia billingiae QL-Z3. Under optimized conditions, its rate of lignin degradation was 25.24% at 1.5 g/L lignin as the sole carbon source. Whole genome sequencing revealed 4556 genes in the genome of QL-Z3. Among 4428 protein-coding genes are 139 CAZyme genes, including 54 glycoside hydrolase (GH) and 16 auxiliary activity (AA) genes. In addition, 74 genes encoding extracellular enzymes are potentially involved in lignin degradation. Real-time PCR quantification demonstrated that the expression of potential ligninolytic genes were significantly induced by lignin. 8 knock-out mutants and complementary strains were constructed. Disruption of the gene for ELAC_205 (laccase) as well as EDYP_48 (Dyp-type peroxidase), ESOD_1236 (superoxide dismutase), EDIO_858 (dioxygenase), EMON_3330 (monooxygenase), or EMCAT_3587 (manganese catalase) significantly reduced the lignin-degrading activity of QL-Z3 by 47–69%. Heterologously expressed and purified enzymes further confirmed their role in lignin degradation. Fourier transform infrared spectroscopy (FTIR) results indicated that the lignin structure was damaged, the benzene ring structure and groups of macromolecules were opened, and the chemical bond was broken under the action of six enzymes encoded by genes. The abundant enzymatic metabolic products by EDYP_48, ELAC_205 and ESOD_1236 were systematically analyzed via liquid chromatography–mass spectrometry (LC–MS) analysis, and then provide a speculative pathway for lignin biodegradation. Finally, The activities of ligninolytic enzymes from fermentation supernatant, namely, LiP, MnP and Lac were 367.50 U/L, 839.50 U/L, and 219.00 U/L by orthogonal optimization. Conclusions Our findings provide that QL-Z3 and its enzymes have the potential for industrial application and hold great promise for the bioconversion of lignin into bioproducts in lignin valorization.

Postoperative pulmonary complications (PPCs) are associated with high mortality rates after lung cancer surgery. Although some studies have discussed the different risk factors for PPCs, the relationship between these factors and their impact on PPCs remains unclear. Hence, this study aimed to systematically summarize the incidence and determine the risk factors for PPCs.We conducted a systematic search of five English and four Chinese databases from their inception to April 1, 2023. A total of 34 articles (8 cohort studies and 26 case-control studies) (n = 31696, 5833 with PPCs) were included in the analysis. The primary outcome was the incidence of PPC. The secondary outcome was the odds ratio (OR) of PPCs based on the identified risk factors calculated by RevMan 5.4. A narrative descriptive summary of the study results was presented when pooling the results or conducting a meta-analysis was not possible.The pooled incidence of PPCs was 18.4 %. This meta-analysis demonstrated that TNM staging (OR 4.29, 95 % CI 2.59–7.13), chronic obstructive pulmonary disease (COPD) (OR 2.47, 95 % CI 1.80–3.40), smoking history (OR 2.37, 95 % CI 1.33–4.21), poor compliance with respiratory rehabilitation (OR 1.64, 95 % CI 1.17–2.30), male sex (OR 1.62, 95 % CI 1.28–2.04), diabetes (OR 1.56, 95 % CI 1.07–2.27), intraoperative bleeding volume (OR 1.44, 95 % CI 1.02–2.04), Eastern Cooperative Oncology Group score (ECOG) > 1 (OR 1.37, 95 % CI 1.04–1.80), history of chemotherapy and/or radiotherapy (OR 1.32, 95 % CI 1.03–1.70), older age (OR 1.18, 95 % CI 1.11–1.24), and duration of surgery (OR 1.07, 95 % CI 1.04–1.10) were significantly associated with a higher risk of PPCs. In contrast, the peak expiratory flow rate (PEF) (OR 0.99, 95 % CI 0.98–0.99) was a protective factor. Clinicians should implement targeted and effective interventions to prevent the occurrence of PPCs.

There are curved bridge structures in the intercity rail line. During the operation of bridges, they are subjected to train loads, resulting in stress amplitudes of the construction materials; during operation, when the train interval is short, the fatigue performance of the bridge should be emphasized. Unlike straight bridges, when a train travels on a curved bridge, it tends to move in the original direction, which undoubtedly causes the train to deviate from the track. Therefore, it is necessary to set the track deflection to limit this movement trend, which will also impart radial forces on the track structure, and the reaction force of this force is called centripetal force. Under the action of centripetal force, the train generates a virtual force called centrifugal force. The material stress amplitude caused by centrifugal force and the vertical force both need to be considered. Therefore, a curved train–bridge coupled system was established to simulate the dynamic stress of the train passing through a curved bridge, and the stress amplitude and cycle number of the dynamic stress time–history curve were analyzed based on the rain-flow method. The cumulative damage of the bridge under different curve radii, different train speeds, different lengths of span, and different operation interval times was analyzed, and the fatigue life was calculated. The results show that the influence of centrifugal force at a small curve radius cannot be ignored. In addition, the cumulative damage and service life are greatly affected by the train speed and bridge span; especially when the train speed is close to the resonance speed, the service life is significantly reduced. Finally, the recommended values for the train passing speed for curved bridges with different spans are given. It was suggested that the design speed of a curved bridge with a span of 25 m, 30 m, and 35 m should be set in the range of 70 to 106 km/h, 78 to 86 km/h, and about 75 km/h, respectively.

Sepsis is a life-threatening organ dysfunction caused by a dysregulated host response to infection, and its morbidity and mortality rates are increasing annually. It is an independent risk factor for intensive care unit-acquired weakness (ICU-AW), which is a common complication of patients in ICU. This situation is also known as sepsis-associated acquired weakness (SAW), and it can be a complication in more than 60% of patients with sepsis. The outcomes of SAW are often prolonged mechanical ventilation, extended hospital stays, and increased morbidity and mortality of patients in ICUs. The pathogenesis of SAW is unclear, and an effective clinical treatment is not available. Ferroptosis is an iron-dependent type of cell death with unique morphological, biochemical, and genetic features. Unlike other forms of cell death such as autophagy, apoptosis, and necrosis, ferroptosis is primarily driven by lipid peroxidation. Cells undergo ferroptosis during sepsis, which further enhances the inflammatory response. This process leads to increased cell death, as well as multi-organ dysfunction and failure. Recently, there have been sporadic reports suggesting that SAW is associated with ferroptosis, but the exact pathophysiological mechanisms remain unclear. Therefore, we reviewed the possible pathogenesis of ferroptosis that leads to SAW and offer new strategies to prevent and treat SAW.

Abstract Sarcopenia is a systemic skeletal muscle disease characterized by a decline in skeletal muscle mass and function. Originally defined as an age‐associated condition, sarcopenia presently also encompasses muscular atrophy due to various pathological factors, such as intensive care unit‐acquired weakness, inactivity, and malnutrition. The exact pathogenesis of sarcopenia is still unknown; herein, we review the pathological roles of the neuromuscular junction and mitochondria in this condition. Sarcopenia is caused by complex and interdependent pathophysiological mechanisms, including aging, neuromuscular junction impairment, mitochondrial dysfunction, insulin resistance, lipotoxicity, endocrine factors, oxidative stress, and inflammation. Among these, neuromuscular junction instability and mitochondrial dysfunction are particularly significant. Dysfunction in neuromuscular junction can lead to muscle weakness or paralysis. Mitochondria, which are plentiful in neurons and muscle fibers, play an important role in neuromuscular junction transmission. Therefore, impairments in both mitochondria and neuromuscular junction may be one of the key pathophysiological mechanisms leading to sarcopenia. Moreover, this article explores the structural and functional alterations in the neuromuscular junction and mitochondria in sarcopenia, suggesting that a deeper understanding of these changes could provide valuable insights for the prevention or treatment of sarcopenia.

Abstract Switchgrass is a promising energy crop has the potential to mitigate global warming and energy security, improve local ecology and generate profit. Its quantitative traits, such as biomass productivity and environmental adaptability, are determined by genotype‐by‐environment interaction (GEI) or response of genotypes grown across different target environments. To simulate the yield of switchgrass outside its original habitat, a genotype‐specific growth model, SwitchFor that captures GEI was developed by parameterising the MiscanFor model. Input parameters were used to describe genotype‐specific characteristics under different soil and climate conditions, which enables the model to predict the yield in a wide range of environmental and climate conditions. The model was validated using global field trail data and applied to estimate the switchgrass yield potentials on the marginal land of the Loess Plateau in China. The results suggest that upland and lowland switchgrass have significant differences in the spatial distribution of the adaptation zone and site‐specific biomass yield. The area of the adaption zone of upland switchgrass was 4.5 times of the lowland ecotype's. The yield difference between upland and lowland ecotypes ranges from 0 to 34 Mg ha−1. The weighted average yield of the lowland ecotype (20 Mg ha−1) is significantly higher than the upland type (5 Mg ha−1). The optimal yield map, generated by comparing the yield of upland and lowland ecotypes based on 1 km2 grid locations, illustrates that the total yield potential of the optimal switchgrass is 61.6–106.4 Tg on the marginal land of the Loess Plateau, which is approximately twice that of the individual ecotypes. Compared with the existing models, the accuracy of the yield prediction of switchgrass is significantly improved by using the SwitchFor model. This spatially explicit and cultivar‐specific model provides valuable information on land management and crop breeding and a robust and extendable framework for yield mapping of other cultivars.

Abstract It is interesting to improve engine system performance with turbocharger technologies. In this study, a systematic simulation for engine and turbocharger matching to provide full utilization of the turbocharger potential and improve the engine performance without sacrificing the emission is developed. A velocity ratio concept was proposed to count the turbocharger performance impacts due to the diameter ratio of compressor and turbine wheels. A design of experiments was used to optimize the turbocharger and engine performance for different turbocharger factors. A better‐matched turbocharger was obtained. A multidisciplinary optimization method was used to design a mixed flow turbine wheel to reduce the turbine velocity ratio at peak efficiency and increase the overall turbocharger efficiency. Results showed that about 0.4% torque improvements and 1.2% reductions in the engine brake‐specific fuel consumption were obtained without making any other changes to the engine. This study demonstrated that systematic simulations for engine systems and considering turbocharger wheel diameter ratio effects could further improve the turbocharged engine system matching and the engine performance.

Concrete is among the most widely used structural materials in buildings and bridges all over the world. During their service life, concrete structures may inevitably display cracks due to long-term fatigue loads, leading to the degradation of structural integrity. Thus, it is very important to detect cracks and their growth in concrete structures using an automated structural health monitoring system. In this paper, experimental research on crack detection and imaging of concrete structures by using sensing skin and electrical resistance tomography (ERT) is presented. Carbon ink is screen-printed on the surface of concrete as a conductive material to form sensing skins. With these sensing skins, when cracks occur on or near the surface, it breaks the continuity of the sensing skins and significantly reduces conductivity in cracking areas. Then, after exciting small currents in sensing skins and measuring related voltage data, an inverse analysis based on total variation (TV) regularization is adopted to reconstruct tomographic images showing conductivity changes in sensing skins, to detect the occurrence and growth of cracks. The effectiveness of conductive sensing skins and our related crack detection method is validated in experimental studies on a concrete beam subjected to fatigue tests.

The Loess Plateau, with a large area of marginal land, holds the potential to produce 62–106 Tg per year of switchgrass biomass; however, the economic feasibility of producing bioenergy in the region is unclear. The farm-gate feedstock production (FGFP) cost of switchgrass was calculated in a spatially explicit way by taking the geographic variation in crop yield, soil properties, land quality, and input costs into consideration in order to evaluate the economic performance of bioenergy production. Cost–supply curves were constructed to explore the energy supply potential of switchgrass feedstock. The calculations were conducted using ArcGIS in a 1 km grid and all the evaluations were conducted under different agricultural management practice (AMP) scenarios in parallel. The FGFP costs showed significant spatial variation ranging from 95 to 7373 CNY (Chinese Yuan) per tonne−1 and that the most economically desirable areas are scattered in the south and southeast region. The weighted average FGFP costs are 710, 1125, and 1596 CNY per tonne−1 for small bale (SB), large bale (LB), and chipping (CP) harvest methods, respectively. The projected energy supply potential is 1927 PJ (Petajoules) per year−1, of which 30–93% can be supplied below the market prices of different fossil fuels according to feedstock formats. Compared to current biomass residual pricing, 50–66 Tg (Teragrams) switchgrass feedstock is competitive. The results demonstrated that the Loess Plateau holds the potential to produce bioenergy that is economically feasible. This study provides a methodological framework for spatially explicit evaluation of the economic performance of perennial energy crops. Detailed information obtained from this study can be used to select the optimal locations and AMPs to produce feedstock production at minimum cost.

Abstract With a large marginal land area, the Loess Plateau in China holds great potential for biomass production and environmental improvement. Identifying suitable locations for biomass production on marginal land is important for decision‐makers from the viewpoint of land‐use planning. However, there is limited information on the suitability of marginal land within the Loess Plateau for biomass production. Therefore, this study aims to evaluate the suitability of the promising perennial energy crop switchgrass (Panicum virgatum L.) on marginal land across the Loess Plateau. A fuzzy logical model was developed and validated based on field trials on the Loess Plateau and applied to the marginal land of this region, owing to its ability of dealing with the continuous nature of soil, landscape variations, and uncertainties of the input data. This study identified that approximately 12.8–20.8 Mha of the Loess Plateau as available marginal land, of which 2.8–4.7 Mha is theoretically suitable for switchgrass cultivation. These parts of the total marginal land are mainly distributed in northeast and southwest of the Loess Plateau. The potential yield of switchgrass ranges between 44 and 77 Tg. This study showed that switchgrass can grow on a large proportion of the marginal land of the Loess Plateau and therefore offers great potential for biomass provision. The spatial suitability maps produced in this study provide information to farmers and policymakers to enable a more sustainable development of biomass production on the Loess Plateau. In addition, the fuzzy‐theory‐based model developed in this study provided a good framework for evaluating the suitability of marginal land.

Abstract High cost of lignocellulases restricts the commercialization of biofuel and bio‐product production from lignocellulosic biomass. Constitutively expressed lignocellulases are considered to degrade cellulose to release small amount of soluble cellodextrins such as cellobiose for further large‐scale production of lignocellulases; however, the underlying mechanism remains to be elucidated. Here, a triple β‐glucosidase mutant of the model fungus Neurospora crassa, which prevents rapid turnover of cellobiose and thus allows the disaccharide to induce lignocellulases, was applied to perform parallel analyses of proteome and phosphoproteome changes in response to cellobiose and Avicel cellulose. The results revealed shared proteome and phosphoproteome responses to cellobiose and Avicel, corroborating the idea that cellobiose mediates the regulation of lignocellulase expression and secretion. The results further suggest that this regulation is achieved at multiple levels, including epigenetic, transcription, post‐transcription, translation, and post‐translation. Proteome profiling revealed that the proteins upregulated by cellobiose and Avicel were over‐represented in cellulose degradation and degradation product transport pathways. Phosphoproteome profiling revealed that the proteins differentially phosphorylated by cellobiose and Avicel were over‐represented by the pathways such as transcriptional control, protein processing and export, cell wall biogenesis, and cellular signaling. Deletion mutation analysis further suggests that the ER chaperon protein Hsp70‐6, the translocation complex subunit Sec66/Sec71, and the signal peptidase subunit Spc2 are involved in lignocellulase secretion, particularly translocation across the endoplasmic reticulum. Altogether, the results offer a new insight into how cellobiose mediates the regulation of lignocellulase expression and secretion, providing a potential strategy for the strain engineering to improve lignocellulase production.

Abstract Background Research into evidence-based practice has been extensively explored in nursing and there is strong recognition that the organizational context influences implementation. A range of barriers has been identified; however, the research has predominantly taken place in Western cultures, and there is little information about factors that influence evidence-based practice in China. The purpose of this study was to explore barriers and facilitators to evidence-based practice in Hunan province, a less developed region in China. Methods A descriptive qualitative methodology was employed. Semi-structured interviews were conducted with staff nurses, head nurses and directors (n = 13). Interviews were translated into English and verified for accuracy by two bilingual researchers. Both Chinese and English data were simultaneously analyzed for themes related to factors related to the evidence to be implemented (Innovation), nurses’ attitudes and beliefs (Potential Adopters), and the organizational setting (Practice Environment). Results Barriers included lack of available evidence in Chinese, nurses’ lack of understanding of what evidence-based practice means, and fear that patients will be angry about receiving care that is perceived as non-traditional. Nurses believed evidence-based practice was to be used when clinical problems arose, and not as a routine way to practice. Facilitators included leadership support and the pervasiveness of web based social network services such as Baidu (百度) for easy access to information. Conclusion While several parallels to previous research were found, our study adds to the knowledge base about factors related to evidence-based practice in different contextual settings. Findings are important for international comparisons to develop strategies for nurses to provide evidence-based care.

Fungal pathogens have evolved combinations of plant cell-wall-degrading enzymes (PCWDEs) to deconstruct host plant cell walls (PCWs). An understanding of this process is hoped to create a basis for improving plant biomass conversion efficiency into sustainable biofuels and bioproducts. Here, an approach integrating enzyme activity assay, biomass pretreatment, field emission scanning electron microscopy (FESEM), and genomic analysis of PCWDEs were applied to examine digestibility or degradability of selected woody and herbaceous biomass by pathogenic fungi. Preferred hydrolysis of apple tree branch, rapeseed straw, or wheat straw were observed by the apple-tree-specific pathogen Valsa mali, the rapeseed pathogen Sclerotinia sclerotiorum, and the wheat pathogen Rhizoctonia cerealis, respectively. Delignification by peracetic acid (PAA) pretreatment increased PCW digestibility, and the increase was generally more profound with non-host than host PCW substrates. Hemicellulase pretreatment slightly reduced or had no effect on hemicellulose content in the PCW substrates tested; however, the pretreatment significantly changed hydrolytic preferences of the selected pathogens, indicating a role of hemicellulose branching in PCW digestibility. Cellulose organization appears to also impact digestibility of host PCWs, as reflected by differences in cellulose microfibril organization in woody and herbaceous PCWs and variation in cellulose-binding domain organization in cellulases of pathogenic fungi, which is known to influence enzyme access to cellulose. Taken together, this study highlighted the importance of chemical structure of both hemicelluloses and cellulose in host PCW digestibility by fungal pathogens.

The extracellular polysaccharide (EPS) matrix embedding microbial cells and soil particles plays an important role in the development of biological soil crusts (BSCs), which is widely recognized as beneficial to soil fertility in dryland worldwide. This study examined the EPS-producing bacterial strains YL24-1 and YL24-3 isolated from sandy soil in the Mu Us Desert in Yulin, Shaanxi province, China. The strains YL24-1 and YL24-3 were able to efficiently produce EPS; the levels of EPS were determined to be 257.22 μg/mL and 83.41 μg/mL in cultures grown for 72 h and were identified as Sinorhizobium meliloti and Pedobacter sp., respectively. When the strain YL24-3 was compared to Pedobacter yulinensis YL28-9T using 16S rRNA gene sequencing, the resemblance was 98.6% and the strain was classified as Pedobacter sp. using physiological and biochemical analysis. Furthermore, strain YL24-3 was also identified as a subspecies of Pedobacter yulinensis YL28-9T on the basis of DNA–DNA hybridization and polar lipid analysis compared with YL28-9T. On the basis of the EPS-related genes of relevant strains in the GenBank, several EPS-related genes were cloned and sequenced in the strain YL24-1, including those potentially involved in EPS synthesis, assembly, transport, and secretion. Given the differences of the strains in EPS production, it is possible that the differences in gene sequences result in variations in the enzyme/protein activities for EPS biosynthesis, assembly, transport, and secretion. The results provide preliminary evidence of various contributions of bacterial strains to the formation of EPS matrix in the Mu Us Desert.

We grew a set of indium and molybdenum co-doped lithium niobate crystals with various indium doping concentrations and investigated their photorefractive properties at different wavelengths (442, 488 and 532 nm). It was found that the diffraction efficiency of 1.0 mol% indium and 0.5 mol% molybdenum co-doped lithium niobate crystal could reach 61.57% at 488 nm. Moreover for 3.0 mol% indium and 0.5 mol% molybdenum co-doped lithium niobate crystal, the response time was greatly shortened to 0.61, 0.76, and 0.74 s at 442, 488, and 532 nm, respectively, while the photorefractive sensitivity reached as high as 7.35 cm/J at 442 nm. These results indicate that co-doping of indium is an efficient way to further enhance the photorefractive properties of molybdenum-doped lithium niobate crystal.

Aims and objectives: To evaluate intensive care unit (ICU) nurses' knowledge of the updated guidelines for the prevention of intravascular catheter-related infections; to identify the factors that affect the nurses' knowledge and to explore the barriers to adherence to evidence-based guidelines in clinical practice in China. Methods: Cross-sectional surveys were carried out in Chinese ICUs from January 2013 to March 2014. The nurses' demographic information, knowledge of the guidelines, and barriers to adherence were assessed by a validated questionnaire and then analyzed statistically. Results: The questionnaires were completed by 455 ICU nurses from 4 provinces of China. The mean score was 8.17 of 20, and higher scores were significantly associated with province, years of experience, and years of ICU experience. Forty-nine (10.7%) nurses had not heard of the guidelines, whereas 231 (50.7%) nurses heard of the guidelines but did not receive training for them. Trained nurses' scores were higher than untrained nurses' scores. The three main barriers to compliance with the guidelines were an unfamiliarity with them, an excessive workload due to a shortage of nurses, and a lack of training. Conclusions: ICU nurses' knowledge of the updated guidelines is quite low, which could be a potential risk factor for patient safety. Multidisciplinary interventions and continuous educational programs are needed to improve the knowledge of and adherence to the guidelines.

We mainly investigated the effect of the valence state of photorefractive resistant elements on the photorefractive properties of codoped crystals, taking the Zn and Mo codoped LiNbO3 (LN:Mo,Zn) crystal as an example. Especially, the response time and photorefractive sensitivity of 7.2 mol% Zn and 0.5 mol% Mo codoped with LiNbO3 (LN:Mo,Zn7.2) crystal are 0.65 s and 4.35 cm/J at 442 nm, respectively. The photorefractive properties of the LN:Mo,Zn crystal are similar to the Mg and Mo codoped LiNbO3 crystal, which are better than the Zr and Mo codoped LiNbO3 crystal. The results show that the valence state of photorefractive resistant ions is an important factor for the photorefractive properties of codoped crystals and that the LN:Mo,Zn7.2 crystal is another potential material with fast response to holographic storage.

A series of LiNbO3: Mo, Hf crystals with 0.5 mol % fixed MoO3 and various HfO2 concentrations (0.0, 2.0, and 3.5 mol %) were grown by the Czochralski technique. The photorefractive properties of the LiNbO3: Mo, Hf crystals were investigated by two-wave coupling measurements and the beam distortion method was employed to obtain the optical damage resistance ability. The UV-visible and OH− absorption spectra were also studied. The experimental results imply that the photorefractive properties of LiNbO3: Mo crystals at laser wavelengths of 532, 488, and 442 nm can be greatly enhanced by doping HfO2 over the threshold concentration. At 442 nm especially, the response time of LN: Mo, Hf3.5 can be shortened to 0.9 s with a diffraction efficiency of 46.07% and a photorefractive sensitivity reaching 6.28 cm/J. Besides this, the optical damage resistance at 532 nm is 3 orders of magnitude higher than that of the mono-doped LiNbO3: Mo crystal, which is beneficial for applying it in the field of high-intensity lasers.

Bismuth-doped lithium niobate (LN:Bi) crystals were grown by Czochralski method and their optical damage resistance, photorefraction, absorption spectra, and defect energy levels were investigated. The experimental results indicate that the photorefractive properties of LN:Bi were enhanced as compared with congruent one, the photorefractive response time was greatly shortened, the photorefractive sensitivity was increased, and the diffraction efficiency of near-stoichiometric LN:Bi (SLN:Bi) reached 31.72% and 49.08% at 532 nm and 488 nm laser, respectively (light intensity of 400 mW/cm2). An absorption peak at about 350 nm was observed in the absorption spectrum of LN:Bi. And the defect energy levels simulation indicates new defect levels appear in the forbidden gap of LN:Bi crystals. Therefore bismuth can act as photorefractive centers in LN crystals.

A series of pure congruent lithium niobate (LiNbO3, CLN) crystals were grown and directly polarized under different electric currents in the growth furnace. Their holographic properties were investigated from the ultraviolet to the visible range. The response time shortened, whereas the diffraction efficiency increased incrementally with the electric current. In particular, the response time of CLN polarized under 100 mA can be reduced by a factor of 10 with a still high saturation diffraction efficiency of about 40.8% at 351 nm. Moreover, its response speed improved by 60 times and 10 times for 473 and 532 nm laser, respectively. The light erasing behavior implies that at least two kinds of photorefractive centers exist in the crystals. Increasing the polarization current induces two pronounced UV absorption peaks and a wide visible absorption peak in CLN crystals. The diffusion effect dominates the photorefractive process and electrons are the dominant carriers. The possible mechanism for the fast photorefractive response is discussed. Increasing the polarization electric current is an effective method to improve the photorefractive response of LN crystal.

A single crystal of Nd3+-doped KGdP4O12 was successfully grown with the top-seeded solution growth and slow cooling (TSSG-SC) technique. It crystallizes in space group C2/c with cell parameters a = 7.812(2) Å, b = 12.307(3) Å, c = 10.474(2) Å, β = 110.84(3)° and Z = 4. The IR and Raman spectra also indicated that the phosphoric polyhedra of Nd:KGdP4O12 has a cyclic symmetry. The chemical composition of the crystal was analyzed and the distribution coefficient of Nd3+ was calculated. The crystal morphology of KGdP4O12 was identified using X-ray diffraction. The compound has good thermal stability to 920°C. Its specific heat and thermal conductivity were determined for potential applications. The spectral properties of Nd:KGdP4O12 indicates that it exhibits broad absorption and emission bands, which are attributed to low symmetry of the crystal. The broad absorption band around 798 nm has a full-width at half-maximum (FWHM) of 14.8 nm and is suitable for AlGaAs laser diode pumping. Moreover, 5 at% Nd3+-doped KGdP4O12 crystal has a long luminescence lifetime of 300 μs and a high quantum efficiency of 96%.

In this paper, we discuss some properties on hyperbolic-harmonic functions in the unit ball of ℂ n . First, we investigate the relationship between the weighted Lipschitz functions and the hyperbolic-harmonic Bloch spaces. Then we establish the Schwarz–Pick type theorem for hyperbolic-harmonic functions and apply it to prove the existence of Landau-Bloch constant for functions in α-Bloch spaces.

Till now it is difficult to obtain a p-type lithium niobate crystal. Here the carrier types in various doped and oxidized LiNbO3 crystals have been investigated by holographic technique. The experimental results show tetravalent ions (Zr4+ and Hf4+) with doping concentrations just above optical damage resistant thresholds is helpful to increase the concentration of holes. And 3.0 mol% ZrO2 doped LiNbO3 can be used as a p-type crystal. The dominant carrier of iron doped LiNbO3 crystal can be changed to holes when treated by thermo-electric oxidization. Then the possible mechanism on how to obtain a p-type LiNbO3 was discussed.