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The numbers of Helly, Carathéodory and Radon are a central theme in classic convex spaces and each of them is invariant under isomorphism. The theory of M-fuzzifying convex spaces is an active research field since it is presented. This paper focus on convex invariants of M-fuzzifying convex spaces. The degrees of Helly independent, Carathéodory independent and Radon independent of non-empty set are defined in the framework of M-fuzzifying convex spaces. By those definitions, we introduce the Helly number, Carathéodory number and Radon number of M-fuzzifying convex spaces. Finally, we inspect M-fuzzifying topology and M-fuzzifying JHC which are characterized by convex invariants of M-fuzzifying convex spaces.

Pyroptosis is an inflammatory cell death form triggered by protease-mediated truncation and release of the N-terminal pore-forming domain of the gasdermin (GSDM) family proteins in various cell types. We report a Bioorthogonally ACtivatable Base editor (BaseBAC) for

Objective: This study is to analyze correlation between traditional Chinese medicine (TCM) syndrome distribution characteristics and prognosis of hypertensive intracerebral hemorrhage (HICH) operation. Methods: In this study, a total of 150 patients who had received HICH operation from April, 2017 to December, 2020 in our hospital and conformed to inclusion standards were selected. According to classification of TCM syndromes, amount of bleeding of patients was recorded through multiple radiological technologies, baseline information was collected, and prognosis was investigated. The final event was long-term follow-up visit of all-cause mortalities. Moreover, correlation between prognosis and TCM syndromes was analyzed. Results: It found through investigations that there are no statistically significant differences in composition ratio of TCM syndromes among patients with different genders and different age ranges (P > 0.05). Among so many TCM syndromes, bleeding amount of patients with declining vitality and distraction is the highest, while bleeding amount of patients with stirring wind due to yin deficiency is the lowest. With respect NIHSS scores, the patients with declining vitality and distraction show the highest NIHSS scores at admission and 10 d of the course of the disease, followed by patients with upward disturbance of wind-fire. There are statistically significant differences among these two groups (P < 0.05). In this study, follow-up visits are performed to all 150 patients and the average follow-up visit time ranges within 2 - 15 months. A total of 13 deaths are reported. Number of all-cause deaths has statistically significant differences among different syndromes (P < 0.05). Conclusions: Evolutionary characteristics of TCM syndromes of HICH are manifested by development from evidence-based symptoms to deficiency syndromes. Different syndromes are correlated with prognosis of patients. On the one hand, this can be used as an objective index for TCM syndrome classification. On the other hand, this is conducive to judge prognosis recovery of patients and to apply the corresponding symptomatic treatment.

Background: Bilateral frontal lobes cerebral contusion and laceration is one unique brain injury in neurosurgery department. It is characteristic of recessive attacking and develops quickly. The unilateral cerebral falx incision is a new minimally invasive surgery that can solve bilateral frontal lobes cerebral contusion and laceration in one surgery. However, it has some limitations in removal of contralateral frontal hematoma and hemostasis due to the limited field of view under the microscope. The unilateral bone window cerebral falx incision of bilateral frontal lobes cerebral contusion and laceration under a neuroendoscopy can acquire a good illumination and field of view. This is beneficial to complete removal of contralateral hematoma, effective hemostasis and retaining brain tissue functions to the maximum extent. Case Presentation: The patient, a 55-year-old man, was hospitalized for “consciousness disorder by 12 h because of car accident”. Physical Examination: Coma, GCS score of E1V2M5, bilateral pupil diameter of 2 mm, presence of light response, contusion of scalp at the left top, peripheral dysphoria and bilateral Bartter syndrome negative. The patient has a history of non-traumatic cerebral stroke 3 years ago. Head CT: Longitudinal fracture of frontal parietal occipital bone, bilateral frontal lobes contusion and laceration, subarachnoid hemorrhage. Diagnosis: Bilateral frontal lobes contusion and laceration, longitudinal fracture of frontal parietal occipital bone, subarachnoid hemorrhage and hematoma of scalp. In emergency treatment, unilateral bone window cerebral falx incision of bilateral frontal lobes cerebral contusion and laceration under a neuroendoscopy was performed. The surgery has achieved satisfying effect. Discussion: This case realized the goal of removing contralateral frontal hematoma through unilateral craniotomy under a neuroendoscopy. Due to the clear field of view, it retained extracerebral layer structures of contralateral olfactory nerve protection frontotemporal completely. Moreover, this surgical technique is conducive to intraoperative recognition of pericallosal arteries and lateral fractured blood vessels. It also involves protection, which conforms to the minimally invasive philosophy. The proposed surgical technology can eliminate contralateral frontal hematoma under a good field of view. However, it is suggested not to manage with the further operation on patients who have brain swelling and difficulties in exposure of cerebral falx. These patients need to determine causes of brain swelling and choose bilateral craniectomy if necessary. Conclusions: Unilateral bone window cerebral falx incision of bilateral frontal lobes cerebral contusion and laceration under a neuroendoscopy is a new application of minimally invasive philosophy in craniocerebral injury operation. It still needs further clinical verifications and experience accumulation.

Objective: This study is to discuss CT performances and direct surgical strategy as well as therapeutic effect of patients who have rupture hemorrhage of intracranial anterior circulation aneurysm with complications of intracranial hematoma and cerebral hernia. Methods: CT performances and treatment of 14 patients who have rupture hemorrhage of intracranial anterior circulation aneurysm with complications of intracranial hematoma and cerebral hernia in our hospital from March, 2019 to March, 2021 were reviewed. The relationship between hematoma caused by intracranial anterior circulation aneurysm and position of the aneurysm was analyzed. Besides, surgical processing keys were discussed. Results: For all selected patients, intracranial hemorrhage is proved to be caused by rupture of aneurysm. Among them, there are 2 cases of anterior communicating aneurysms, 3 cases of posterior communicating aneurysms, and 9 cases of middle cerebral aneurysms. According to exploration, we found 1 case of multiple aneurysm, which is the combination of a middle cerebral aneurysm (the responsible aneurysm) and ipsilateral posterior communicating aneurysm. There were two cases of intraoperative rupture. In this study, 3 patients died. According to GOS grading at 3 months after the operation, there were 1 case of V-grade (good recovery), 3 cases of IV-grade (self-maintenance), 5 cases of III-grade (severe disabled), 2 cases of II-grade (persistent vegetative state) and 3 cases of I-grade (died). Conclusions: Emergency microsurgical treatment can lower the death rate of cerebral hernia caused by intracranial anterior circulation aneurysm with intracranial hematoma and recover the neurological functions to the maximum extent.

Objective: This study is to observe the therapeutic effects of Shenmai injection on patients with hypertensive ICH and cerebrocardiac syndromes as well as its effects on prognosis. Methods: A total of 72 patients with severe hypertensive ICH and cerebrocardiac syndromes were selected and divided into the control group and the experimental group (Shenmai injection group) randomly, 36 patients in each group. The control group was treated by conventional therapy, while the experimental group was administrated by intravenous drip of the mixture of 50 ml Shenmai injection and 250 ml 5% glucose injection based on the treatment of the control group, once everyday, 2 weeks continuously. Results: Myocardial enzyme indexes of two groups at 3d, 7d and 14d were observed (Table 1). Myocardial enzyme index of the experimental group was improved quickly and significantly. Myocardial enzyme data of two groups at 3d, 7d and 14d were P < 0.05. According to the electrocardiographic examinations results, ECG anomalies of the experimental group at 7d and 14d were also improved quickly (P < 0.05). There are no evident anomalies in GCS scores of two groups at 7d, but GCS score of the experimental group at 14d of the medication was significantly higher than that of the control group (P < 0.05). Conclusions: Shenmai injection has some therapeutic effects on cerebral injuries and myocardial damages. It can treat brain and heart simultaneously, realize the goal of addressing both symptoms and root causes, and improve prognosis of patients with severe hypertensive ICH.

Mesenchymal Stem Cells (MSCs) are a type of non-hematopoietic progenitor cells which have self-replication capacity and multilineage differentiation. They have widely applied in studies of various diseases due to their effects in damaged tissue repair, neuroprotection and immunoregulation. MSCs can secret exosomes through multiple ways in the physiological or pathological state. Many researches’ results on MSC-Exo show that it possesses many functions similar to MSCs, such as immunoregulation and regeneration promotion of damaged tissues. Hence, MSC-Exo is believed to have considerable research potentials in regenerative medicines. This study reviewed the research progresses on biological characteristics and functions of MSC-Exo.

Long-span suspension bridges are widely used in deep valleys, which face severe seismic risk. However, the potential saddle-cable frictional slippage under earthquake excitation as well as its influence on the seismic response of the whole suspension bridge has not yet been investigated. To investigate the effect of frictional slippage at the saddle-cable interface, this paper developed a nonlinear numerical model that considers the saddle-cable slippage. Another contrasting model with a non-slipping saddle-cable interface was used for quantitative comparison. Nonlinear dynamic analyses were conducted using these two models. The saddle-cable interfacial response indicated the realization of the frictional slippage at the saddle-cable interface under the maximum considered earthquake. The overall damage patterns, critical sectional performance, main girder drift, and energy dissipation were discussed in detail. Under the design based and maximum considered intensities, the saddle-cable slippage was seldom observed. The visible frictional slippage was encountered only at ultimate safety earthquake, which could be helpful to limit the transferred load, protect the pylon from yielding, and dissipate approximately 14% of the input seismic energy. While the slippage could not evidently affect the overall deformation pattern of the suspension bridge, as well as the response of bearings and central buckles.

Plants produce a number of biological active substances with healthy benefits. Resveratrol (3,5,4′-trihydroxystilbene), a polyphenol produced by plants has been associated with many health beneficial properties, including its ability to induce mitochondrial biogenesis and fight against health problems such as obesity, inflammation, heart diseases, cancers among others. Mitochondrial dysfunction is recognized as central to the pathogenesis and development of many diseases. Thus the present review describes how resveratrol (RSV) may counteract physiological and age-related diseases/disorders through its effect on mitochondrial biogenesis and function. In addition, we discuss the chemistry, main sources, and the doses of RSV shown in previous studies to be efficient for the prevention and treatment of different diseases. Through its ability to improve mitochondrial dysfunction, RSV can be used in the prevention and/or treatment of human physiological diseases. However, more research for optimal dose in a human scale is still relevant. This review brings new hope to the therapy of physiological diseases as it will provide useful future perspectives for the planning of clinical studies on RSV and mitochondrial dysfunction-related diseases.

The abundance of bacterial effectors have inspired us to explore their potential in rewiring malignant cell signaling. Their incapability for entering cells, however, hinders such application. Herein we developed a cationic lipid-based high throughput library screening platform for effective intracellular delivery of bacterial effectors. As the misregulated MAPK signaling is a hallmark of many types of cancer, we turned to the Shigella effector OspF which irreversibly inactivates ERK, the terminal component of MAPK cascade. We created a function-based screening assay to obtain AMPA-O16B lipid nanoparticles for effective OspF intracellular delivery, which inhibited the malignant MAPK signaling and tumor growth in vitro and in vivo. Furthermore, the optimized lipid nanoparticle formulation can deliver OspF to modulate the immunosuppressive responses in macrophages. Our work is a general strategy to explore the therapeutic potentials of naturally evolved bacterial effectors.

Oxidized tyrosine products (OTP) have been detected in commercial foods with high protein content, such as meat and milk products. OTP intake induces tissue oxidative stress and affects the normal activity of the hypothalamic-pituitary-thyroid axis (HPT). This study aims to investigate the effects of OTP and their main product, dityrosine (Dityr), on mouse myocardial function and myocardial energy metabolism. Mice received daily intragastric administration of either tyrosine (Tyr; 420 μg/kg body weight), Dityr (420 μg/kg body weight), or OTP (1909 μg/kg body weight) for 35 days. Additionally, H9c2 cells were incubated with various concentrations of Dityr for 72 h. We found that OTP and pure Dityr induced oxidative stress in growing mice and in H9c2 cells, resulting in a redox state imbalance, myocardial injury, mitochondrial dysfunction, and energy metabolism disorder. Dityr interferes with T3 regulation of the myocardium via the PI3K/AKT/GSK3β pathway, leading to myocardial mitochondrial damage and energy metabolism disorders. Food-borne OTP, especially Dityr, can disrupt thyroid hormone function in mouse myocardia leading to mitochondrial dysfunction, energy metabolism disorder, and oxidative stress.

The aim of this study is to compare the recurrence rates with different modalities used as first-line treatment for first-episode primary spontaneous pneumothorax, and investigated the risk factors for recurrence. We retrospectively reviewed consecutive patients under 60 years of age who were treated for a first episode of primary spontaneous pneumothorax between May 1996 and May 2016 at our hospital. The patients were divided into 6 groups according to the treatment received. A total of 667 patients with 890 episodes of primary spontaneous pneumothorax were included in the analysis. The median follow-up period was 64.5 months. The recurrence rate in the 6 groups was as follows: observation, 52.5% (21/40); chest tube drainage, 32.6% (84/258); aspiration, 100% (42/42); axillary thoracotomy, 8.8% (9/102); 2-port video-assisted thoracoscopic surgery (VATS), 6.0% (7/117); and 3-port VATS, 10.6% (11/108). The recurrence rate was higher in non-surgery groups than in surgery groups (147/340 vs 27/327, P

Changes in leaf length, width, area, weight, chlorophyll and carotenoids contents, and photosynthetic variables with different leaf positions were investigated in fruit cucumber. Plants were grown on rockwool slabs in an environmentally controlled greenhouse and irrigated by drip fertigation. Leaf measurements were conducted from the first to the 15th leaf (the oldest to the youngest). The results showed that fresh weight per unit leaf area decreased from the second to the 15th leaf. Changes in cucumber leaf length, width, and area followed quadratic models from the first to the 15th leaf. The quadratic models of leaf length, width, and area fit the measurements well, with R2 values of 0.925, 0.951, and 0.955, respectively. The leaf chlorophyll a and b and carotenoid contents increased from the oldest leaf (first leaf) to the youngest leaf and decreased after reaching the highest values. Changes in the net photosynthetic rate (Pn) also followed the quadratic model from the first to the 15th leaf, with R2 values of 0.975. The leaf transpiration rate (Tr) increased from the first to the 14th leaf. Our results revealed patterns in leaf growth and photosynthetic changes at different leaf positions in fruit cucumber and improved our understanding of the growth and development of fruit cucumber in the greenhouse production system.

Background: Patients with severe hypertensive brainstem hemorrhages have poor prognoses if they only receive conservative medical management. In contrast, aggressive operative interventions may decrease the morbidity and mortality in such patients. These operative treatments include craniotomy for evacuation of the hematoma, stereotactic positioning, and neuronavigational-guided hemorrhage puncture and drainage. Here, we report a novel and relatively simple procedure to achieve satisfactory outcomes in a patient with a brainstem pontine hemorrhage. Case Presentation: A 53-year-old man who was diagnosed with brainstem pontine hemorrhage. On hospital day 6, he underwent CT-guided, virtual stereotactic puncture and catheter drainage of this brainstem pontine hemorrhage. Medical treatments were continued after this procedure. On postoperative day 16 (hospital day 22), the patient was discharged from the hospital, awake and able to answer questions appropriately. Muscle strengths were grades V and IV for the left and right extremities, respectively. The patient was continued with active rehabilitation and achieved a Barthel index of 85 points at one month after the percutaneous drainage procedure. Conclusion: CT-guided, virtual stereotactic percutaneous transcranial puncture and catheter drainage for brainstem pontine hemorrhages has obvious potential advantages and offers a possible alternative to achieve the best outcomes with minimal operative trauma compared to open microcraniotomy.

This paper proposes a multimodal deep learning method for forecasting the daily power generation of small hydropower stations that considers the temporal and spatial distribution of precipitation, which compensates for the shortcomings of traditional forecasting methods that do not consider differences in the spatial distribution of precipitation. First, the actual precipitation values measured by ground weather stations and the spatial distribution of precipitation observed by meteorological satellite remote sensing are used to complete the missing precipitation data through linear interpolation, and the gridded precipitation data covering a group of small hydropower stations are constructed. Then, considering the time lag between changes in the daily power generation of the group of small hydropower stations and precipitation, the partial mutual information method is used to estimate the “time difference” between the two, and combined with the precipitation grid data, a data set of the temporal and spatial distribution of precipitation is generated. Finally, using only the temporal and spatial distribution of precipitation and historical power generation data, a multimodal deep learning network based on a convolutional neural network (CNN) and multilayer perceptron (MLP) is constructed, and a highly accurate prediction model for the daily power generation of small hydropower stations is obtained. Taking the real power generation data of a group of small hydropower stations in southern China as an example, after considering the temporal and spatial distribution of precipitation, the prediction accuracy of the proposed method is as high as 93%, which is approximately 5.8% higher than before considering the temporal and spatial distribution of precipitation. In addition, compared with mainstream methods such as support vector regression (SVR) and the long–short-term memory network (LSTM) (the average accuracy is about 87%), and the average accuracy improvement of the proposed method is approximately 6%.

To investigate the efficacy of Cigu Xiaozhi pill (, CGXZ) on non-alcoholic steatohepatitis (NASH)-associated lipoapoptosis through the stress-activated c-Jun N-terminal kinase (JNK)/ stress-activated protein kinase signalling pathway.Sixty male Sprague-Dawley rats were randomly divided into the following groups (10rats each): blank control, model, low-dose CGXZ, medium-dose CGXZ, high-dose CGXZ, and positive control (treated with SP600125, a JNK inhibitor). The NASH model was established and the histomorphological characteristics of haematoxylin and eosin-stained liver tissues were examined under a light microscope. Cell apoptosis in liver tissues was assessed via terminal deoxynucleotidyl transferase dUTP nick-end labelling assay. In addition, the mRNA and protein expression levels of p-JNK, p-c-Jun, caspase-8, Fas, and Fas-L were determined via fluorescence-based quantitative real-time PCR, immunohistochemical and Western blot assays.Histopathological examination of the liver showed that the model rats had moderate-to-severe steatosis with infiltration of inflammatory cells as well as significantly higher expression levels of the p-JNK, p-c-Jun, caspase-8, Fas, and Fas-L proteins, compared with those in the blank control group (P0.01). Hepatic lobules of the rats in the treatment groups showed significantly reduced vacuolar degeneration and steatosis as well as alleviated inflammatory cell infiltration. The high and medium-dose CGXZ groups exhibited significantly lower mRNA and protein expression levels of p-JNK, p-c-Jun, caspase-8, Fas, and Fas-L, compared with those in the model group (P0.05 or P0.01).CGXZ pill inhibited the onset of hepatocyte apoptosis by regulating the expression of p-JNK, p-c-Jun, caspase-8, Fas, and Fas-L, thereby exerting therapeutic effects against NASH.

An architecture of power geographical wiring system based on Mashup mode is proposed, which solves the problems of the insufficient accuracy and data dimension, the difficulty of updating, the complicated drawing steps and the repetitive work of the traditional geographical wiring diagram, and greatly improves the drawing efficiency and accuracy of the geographical wiring diagram. Using the tile pyramid model map as the platform, Web Service technology and HTTPS transmission protocol are used to fuse and display the geographical information and real-time running status information of power lines and devices, realizing the visualization of geographical information, running status and fault location of lines and devices. The data transmission reduction rate of map in the municipal scale system can be up to 98.06%, which greatly reduces the network data transmission, realizes the whole system response within 500ms, and greatly improves the user experience. The proposed geographical wiring system architecture has been running online for nearly 2 years in a certain city of Guangxi. The system is stable, safe and reliable, and has a wide application prospect.

This paper proposes a power television and tele-dialogue system architecture based on the virtual private cloud. The architecture uses HTML5 technology to replace NPAPI plug-in technology, which eliminates the network security threats caused by the defects of NPAPI plug-ins, and dramatically improves the security of the system. With the help of technologies such as WebRTC and WebSocket, real-time communication of the browser is realized, the waste of terminal equipment and server performance is reduced, and the security of data transmission is improved. Compared with the traditional system architecture, the average total connection time of this system is only 10.912%, which significantly reduces the response time of the system. The proposed architecture promotes the power dispatching system from the traditional four to the current six types of “tele-” functions and gives better play to the role of cloud dispatching system in power production, operation, and management.

Noni (Morinda citrifolia L.) is rich in polyphenols, flavonoids, terpenoids, and iridoids. However, its bad taste and smell make noni fruit unsuitable for consumption. After fermentation, noni wine becomes free from the undesirable smell. Nevertheless, it is still unclear whether processed noni could retain its original nutrients and effects. Therefore, we conducted a series of evaluations on the nutritional composition and efficacy of noni wine. Our results showed that the polyphenol, flavonoid, and vitamin C contents in noni wine were 558.80, 234.42, and 0.30 mg/L, respectively. Our animal experiments showed that 40 ml kg-1 day-1 noni wine could reduce bodyweight, as well as the levels of body fat, serum triglycerides, total cholesterol, and low-density lipoprotein, while it simultaneously increased the amount of energy expenditure and activity, and improved the systemic antioxidant capacity in mice following a high-fat diet. The results of the gene expression and western blot analyses showed that 40 ml kg-1 day-1 noni wine could regulate the Nrf2 pathway and improve the antioxidant enzyme gene expression in mice maintained on a high-fat diet, thereby improving body lipid metabolism, reducing fatty acid synthesis, and promoting fatty acid β-oxidation. Our study indicated that drinking 40 ml kg-1 day-1 noni wine could effectively prevent high-fat diet-induced oxidative stress and obesity in mice. PRACTICAL APPLICATIONS: Noni fruit is rich in nutrients but its bad smell and hardship of processing make its commercialization difficult. Previous studies mainly focused on fresh noni juice and its primary processed products, while few noni products, of poor taste and low quality, are available in the market. Therefore, the fruit wine with both the nutritive values and the special flavor of noni has broad market prospects. Our work provides a valuable reference for the commercialization of noni wine.

NADH cytochrome b(5) reductase mediates electron transfer from NADH to cytochrome b(5) utilizing flavin adenine dinucleotide as a redox cofactor. Reduced cytochrome b(5) is an important cofactor in many metabolic reactions including cytochrome P450-mediated xenobiotic metabolism, steroid biosynthesis and fatty acid metabolism, hemoglobin reduction, and methionine and plasmalogen synthesis. Using recombinant human enzyme, we discovered that cytochrome b5 reductase mediates redox cycling of a variety of quinones generating superoxide anion, hydrogen peroxide, and, in the presence of transition metals, hydroxyl radicals. Redox cycling activity was oxygen-dependent and preferentially utilized NADH as a co-substrate; NADH was 5–10 times more active than NADPH in supporting redox cycling. Redox cycling activity was greatest for 9,10-phenanthrenequinone and 1,2-naphthoquinone, followed by 1,4-naphthoquinone and 2-methyl-1,4-naphthoquinone (menadione), nitrofurantoin and 2-hydroxyestradiol. Using menadione as the substrate, quinone redox cycling was found to inhibit reduction of cytochrome b(5) by cytochrome b(5) reductase, as measured by heme spectral changes in cytochrome b(5). Under anaerobic conditions where redox cycling is inhibited, menadione had no effect on the reduction of cytochrome b(5). Chemical redox cycling by cytochrome b(5) reductase may be important in generating cytotoxic reactive oxygen species in target tissues. This activity, together with the inhibition of cytochrome b(5) reduction by redox-active chemicals and consequent deficiencies in available cellular cytochrome b(5), are likely to contribute to tissue injury following exposure to quinones and related redox active chemicals.

High consumption of cooking oils in modern society is believed to be the major cause of cardiovascular disease. The effect of cooking oils depends on their fatty acid composition. Therefore, it is important to blend different types of oils to improve functional properties. In this study we evaluated the effects of a functional blended oil (BO) composed of a high level of oleic acid (OA) (50.93%) and α-linolenic acid (ALA) (5.41%) on cardiovascular health, blood pressure (BP) and body weight (BW) in comparison with lard oil (LO) and peanut oil (PO). Ninety male Wistar rats were divided into three groups and fed for 12 weeks with BO, LO and PO. Each group was divided into low, middle and high fat groups and fed with oil supply ratios of 6.7%, 10.9% and 48.1%, respectively. After the feeding period, the rats were sacrificed, and data were collected and analyzed. Rats treated with BO especially at a high dose (HBO) showed a significantly lower body weight, fat weight, liver weight, fat ratio, food intake and energy intake. BO significantly reduced n-6/n-3 levels in plasma, liver and adipose tissues as well as serum triglycerides (TGs) and low density lipoprotein cholesterol (LDL-C) but contrarily increased high density lipoprotein cholesterol (HDL-C). Furthermore, HBO treatments decreased mRNA expression of lipid anabolism-related genes, lipid inflammatory-related genes, toll-like receptor 4 (TLR4), nuclear factor kappa beta (NF-κB) and monocyte chemoattractant protein (MCP)-1 and increased lipid catabolism-related genes as well as peroxisome proliferator-activated receptor gamma mRNA (PPARγ mRNA). In terms of antioxidant enzymes, BO treatment has increased the activity of catalase (CAT), glutathione peroxidase (GPx) and superoxide dismutase (SOD) by lowering malondialdehyde (MDA) and reactive oxygen species (ROS) in tissues. Moreover, BO supplementation showed a significant lowering effect on the serum levels of C-reactive protein (CRP) and tumor necrosis factor alpha (TNF-α), blood pressure (BP), atherosclerosis index (AI) and heart rate (HR). These findings highlight that the new blend of canola oil, corn oil, olive oil, peanut oil and sunflower oil with a low n-6/n-3 PUFA ratio of 6 : 1 could prevent and control cardiovascular disease (CVD).

Dopaminergic (DAergic) hypofunction in the medial prefrontal cortex (mPFC) has been implicated in the negative and cognitive symptoms of schizophrenia and is regulated by serotonergic (5-HTergic) neurons in the dorsal raphe nucleus (DRN). The lateral habenula (LHb) is a key element in controlling DRN 5-HT neurons. We investigated how the LHb impacts the activity of mPFC neurons and whether it mediates the involvement of DRN on development of symptoms in a pharmacological animal model of schizophrenia. We used immunohisochemistry to assess cytochrome-c oxidase (COX) activity of the LHb in MK-801 model rats and extracellular firing recording to compare firing rates in LHb neurons of acute MK-801-treated rats. The sucrose preference, social interaction, and radial arm maze tests were used to access schizophrenia-like behavior in rats with electrolytically lesioned LHb. Finally, we examined levels of the dopamine D1 receptor (D1R) and tyrosine hydroxylase (TH) in the mPFC, and tryptophan hydroxylase 2 (TPH2) in the DRN of rats with LHb lesions to determine the possible mechanism underlying the schizophrenia-like behavior associated with lesioned LHb. We found that COX levels and LHb neuron firing rates decreased significantly in MK-801-treated animals. The LHb lesions induced negative and cognitive, but not positive symptoms of schizophrenia. The D1R and TH levels decreased in the mPFC while TPH2 expression elevated in the DRN and mPFC of LHb-lesioned rats. These results suggest that LHb hypoactivity may contribute to the negative and cognitive symptoms of schizophrenia by downregulating D1R expression in the mPFC, which might be mediated by DRN 5-HT neurons.

The complicated and entangled cell signaling network is dynamically regulated by a wide array of enzymes such as kinases. It remains desirable but challenging to specifically modulate individual, endogenous kinases within a cell, particularly in a spatial–temporally controlled fashion. Current strategies toward regulating the intracellular functions of a kinase of interest either lack specificity or require genetic engineering that may perturb its physiological activity. Herein, we harnessed a bacterial effector OspF for optical and chemical modulation of the endogenous mitogen-activated protein kinase (MAPK) cascade in living cells and mice. The phospho-lyase OspF provided high specificity and spatial resolution toward the desired kinase such as the extracellular signal-regulated kinase (ERK), while the genetically encoded bioorthogonal decaging strategy enabled its temporal activation in living systems. The photocaged OspF (OspF*) was applied to dissect the subcellular signaling roles of ERK in nucleus as opposed to cytoplasm, while the chemically caged OspF (OspFc) was introduced into living mice to modulate ERK-mediated gene expression. Finally, our spatially and chemically controlled OspFc was further used to precisely tune immune responses in T cells. Together, our bioorthogonal engineering strategy on bacterial effectors offers a general tool to modulate cell signaling with high specificity and spatial–temporal resolution., Bioorthogonally caged bacterial phospho-lyases allow precise modulation of endogenous kinases and cell signaling via small molecules and light with high spatial−temporal resolution.

In this paper, a hybrid nanocomposite (MB-rGO) was synthesized based on the π–π stacking interactions between methylene blue (MB) and reduced graphene oxide (rGO). The as-synthesized nanocomposite was characterized by SEM, TEM, XRD, FTIR, UV-vis and XPS spectra. UV-vis spectroscopy and electrochemical tests suggested the MB-rGO modified on the electrode exhibited glucose oxidase-mimetic catalytic activity towards glucose, and displayed excellent electrocatalytic performance for electrochemical detection of glucose with a wide linear range from 1.04 to 17.44 mM, a low detection limit of 45.8 μM and a large sensitivity of 13.08 μA mM−1 cm−2. The proposed glucose sensor also showed high stability, reproducibility and good abilities of anti-interference to dopamine, ascorbic acid and uric acid. Moreover, the modified electrode was used to determine glucose concentration in human blood serum samples with satisfactory results.