24 results on '"Shang-li, Liu"'
Search Results
2. [Treatment of severely osteoporotic vertebral compression fractures with the vertebral body stent system and percutanous kyphoplasty combined with zoledronic acid]
- Author
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Xiang-Yang, Xu, De-Min, Luo, Shang-Li, Liu, Xiao-Tao, Shen, Zu-Yan, Zhou, and Guo-Dong, Yuan
- Subjects
Aged, 80 and over ,Male ,Bone Cements ,Middle Aged ,Zoledronic Acid ,Treatment Outcome ,Fractures, Compression ,Humans ,Spinal Fractures ,Female ,Kyphoplasty ,Stents ,Osteoporotic Fractures ,Aged ,Retrospective Studies - Abstract
To investigate the clinical efficacy of vertebral body stent (VBS) system and percutanous kyphoplasty (PKP) combined with zoledronic acid for the treatment of severely osteoporotic compression vertebral fractures (OVCFs).The clinical data of 48 patients with osteoporotic thoracolumbar fractures treated from December 2017 to December 2018 were retrospectively analyzed, including 13 males and 35 females, aged 55 to 92 years old with an average (71.2±10.5) years. All patients were treated with VBS system PKP surgery, and zoledronic acid injection was used for anti-osteoporosis treatment after operation. The VAS scores ODI, the height of diseasedvertebral lost were compared before operation, 3 d and half a year after operation, and whether there was re-fracture of diseased or adjacent vertevrae after operation was observed.Before operation, 3 d and half a year after operation, VAS scores were 7.60±0.12, 3.00±0.46, 1.20±0.23, ODI were(82.00±0.32)%, (30.00±1.50) %, (18.00±0.16) %, the height of diseased vertebral lost were (12.00±0.43) mm, (3.00± 0.15) mm, (3.60±0.51) mm respectively. Postoperative VAS score, ODI, the height of diseased vertebral lost were obviously improved (VBS system and PKP combined with zoledronic acid in the treatment of OVCFs not only may effectively relieve the pain in the thoracolumbar back, improve the mobility of the thoracolumbar, but also can restore the height of the vertebral body to the maximum extent, and prevent the re-fracture of the affected vertebrae and adjacent vertebrae, which is worthy to spread in clinic.
- Published
- 2020
3. Genetic diversity and population structure of
- Author
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Shuang, Li, Shang-Li, Liu, Si-Yu, Pei, Man-Man, Ning, and Shao-Qing, Tang
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Camellia huana ,cpDNA, chloroplast DNA ,TPM, two-phased model ,Genetic diversity ,SSC, small single-copy ,PCR, polymerase chain reaction ,AMOVA, analysis of molecular variance ,SSR, simple sequence repeat ,PCoA, principal coordinate analysis ,Conservation implications ,Genetic structure ,CTAB, cetyl trimethylammonium bromide ,Research Paper ,SMM, stepwise mutation model - Abstract
Camellia huana is an endangered species with a narrow distribution in limestone hills of northern Guangxi and southern Guizhou provinces, China. We used one chloroplast DNA (cpDNA) fragment and 12 pairs of microsatellite (simple sequence repeat; SSR) markers to assess the genetic diversity and structure of 12 C. huana populations. A total of 99 alleles were detected for 12 polymorphic loci, and eight haplotypes and nine polymorphic sites were detected within 5200 bp of cpDNA. C. huana populations showed a low level of genetic diversity (n = 8, Hd = 0.759, Pi = 0.00042 for cpDNA, NA = 3.931, HE = 0.466 for SSRs), but high genetic differentiation between populations (FST = 0.2159 for SSRs, FST = 0.9318 for cpDNA). This can be attributed to the narrow distribution and limestone habitat of C. huana. STRUCTURE analysis divided natural C. huana populations into two groups, consistent with their geographical distribution. Thus, we suggest that five natural C. huana populations should be split into two units to be managed effectively.
- Published
- 2019
4. [Study on early screening of high saponin ginseng lines]
- Author
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Li-Shan, Hao, Yu-Gang, Gao, Pu, Zang, Yan, Zhao, Zhong-Mei, He, Hong-Yan, Zhu, He, Yang, Shang-Li, Liu, Jing-Ting, Liu, and Lian-Xue, Zhang
- Subjects
Plant Breeding ,Panax ,Glycerolphosphate Dehydrogenase ,Saponins ,Polymerase Chain Reaction ,DNA Primers ,Drugs, Chinese Herbal - Abstract
The study aims at screening the specific bands by PCR, quickly and accurately evaluating the quality of ginseng seeding, accelerating the process of ginseng breeding. Based on the correlation of genetic differences and saponin content between individuals, a pair of specific primer GC1 was screened by PCR. According to the experiment by L16 (45) orthogonal test, a PCR system most suitable for GC1 was established, which came out total 25 μL reaction system containing DNA 2.60 mg•L⁻¹, Mg²⁺ 1.44 mmol•L⁻¹, dNTP 0.19 mmol•L⁻¹, primer 0.32 μmol•L⁻¹ and Taq enzyme concentration 0.076 U•μL⁻¹. By comparing the saponin content and the GC1 PCR electrophoretogram of samples, the ginseng, with 1 200 bp specific band by PCR of GC1, the contents of 9 monosodium saponins and their additions were higher than others, which provided a reliable method for accelerating the process of ginseng breeding. The sequence was sequenced and 99% homologous to glycerol-3-phosphate dehydrogenase.
- Published
- 2017
5. Correlation Between MR Imaging and Discography With Provocative Concordant Pain in Patients With Low Back Pain
- Author
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Shang-li Liu, Qing-yu Liu, Yue Ding, Jian-yu Chen, Zehong Yang, Rui-yan Lv, and Jian-bin Huang
- Subjects
Adult ,Male ,China ,medicine.medical_specialty ,Statistics as Topic ,Provocation test ,Discography ,Comorbidity ,Risk Assessment ,Risk Factors ,Prevalence ,medicine ,Humans ,In patient ,medicine.diagnostic_test ,business.industry ,Magnetic resonance imaging ,Middle Aged ,Magnetic Resonance Imaging ,Mr imaging ,Low back pain ,Surgery ,Anesthesiology and Pain Medicine ,Nociception ,Female ,Lumbar spine ,Neurology (clinical) ,medicine.symptom ,business ,Nuclear medicine ,Low Back Pain ,Intervertebral Disc Displacement - Abstract
Objective: This study aimed to correlate magnetic resonance (MR) findings and discography with pain response at provocative discography in patients with low back pain. Methods: Ninety-three patients who underwent MR imaging of the lumbar spine and subsequent provocation discography as part of a clinical evaluation of low back pain were enrolled in the study. MR images were then evaluated for disc degeneration, high-intensity zone (HIZ), and endplate abnormalities. In the procedure of discography, concordant pain was denoted as positive, whereas discordant pain and no pain were denoted as negative. Finally, MR and discographic findings were analyzed by x 2 test based on results of concordant pain. Results: Discography was conducted on 256 discs successfully, 116 discs of which presented with concordant pain, and the others presented with discordant pain. There were 141 discs were evaluated as Grade I-III on MR images, 17 of which presented with concordant pain; 115 were evaluated as Grade IV-V, 99 of which presented with concordant pain. HIZ was found in 60 discs, 52 of which had concordant pain. The endplate abnormalities were observed in 58 discs, 51 of which manifested concordant pain. Concordant pain was significant correlated with Type IV-V discs on discography (χ 2 = 144.08, r = 0.60, P < 0.01), Grade IV-V disc degeneration on MR image (χ 2 = 137.11, r = 0.59, P < 0.01), the presence of HIZ (χ 2 = 51.93, r = 0.41, P < 0.01), and endplate abnormalities (χ 2 = 52.76, r = 0.41, P < 0.01). Discussion: Disc degeneration grades on MR imaging showed an association with discographic grades. Type IV-V discs on discography, Grade IV-V disc on MR images, the presence of HIZ, and endplate abnormalities might indicate discogenic pain in patients with chronic low back pain.
- Published
- 2011
6. Double role of Fas ligand in the apoptosis of intervertebral disc cells in vitro
- Author
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Xinguang Wang, Dongshang Huang, Shang-li Liu, Li-yang Cui, Yue Ding, Gang Wang, Dunfu Han, and In-Cheong Si
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Biophysics ,hemic and immune systems ,chemical and pharmacologic phenomena ,Tumor Necrosis Factor Ligand Superfamily Member 6 ,Intervertebral disc ,General Medicine ,Biology ,Biochemistry ,In vitro ,Fas ligand ,Cell biology ,law.invention ,medicine.anatomical_structure ,Immune privilege ,law ,RNA interference ,Apoptosis ,Immunology ,Recombinant DNA ,medicine - Abstract
Fas ligand (FasL) may play an important role in maintaining the immune privilege of intervertebral disc (IVD). Besides, it is closely related to the apoptosis of degenerative disc cells. Nowadays, lots of reports have described about the paradoxical effects of FasL, although the effect of FasL on IVD cells is still under debate. In this study, we tried to investigate the effects of FasL on Fas expression and on the apoptosis of nucleus pulposus (NP) cells in Sprague-Dawley rats. The results showed that the expression of Fas in NP cells was significantly increased by the recombinant FasL. Meanwhile, the apoptosis of NP cells increased markedly in a FasL dose-dependent manner. Interestingly, RNA interference results indicated that the increase of Fas expression and the NP cell apoptosis described previously were inhibited by Fas siRNA, suggesting that RNA interference might be one of novel strategies to prevent IVD cells from apoptosis.
- Published
- 2009
7. IL-1beta sensitizes rat intervertebral disc cells to Fas ligand mediated apoptosis in vitro
- Author
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Li-yang Cui, Ruofan Ma, Dongsheng Huang, Qiu-hui Pan, Yue Ding, Bao-shan Hu, Wei-guo Huang, and Shang-li Liu
- Subjects
Male ,Fas Ligand Protein ,Interleukin-1beta ,Apoptosis ,Fas ligand ,Flow cytometry ,Rats, Sprague-Dawley ,chemistry.chemical_compound ,Annexin ,medicine ,Animals ,Pharmacology (medical) ,RNA, Messenger ,fas Receptor ,Propidium iodide ,Intervertebral Disc ,Cells, Cultured ,Pharmacology ,Dose-Response Relationship, Drug ,medicine.diagnostic_test ,Reverse Transcriptase Polymerase Chain Reaction ,Chemistry ,Drug Synergism ,General Medicine ,Flow Cytometry ,Fas receptor ,Molecular biology ,In vitro ,Rats ,Up-Regulation ,Immunology ,Fetal bovine serum - Abstract
To determine the apoptotic effect of recombinant rat Fas Ligand on rat intervertebral disc cells pre-treated with IL-1beta in vitro, and the expression of Fas in cultured rat intervertebral disc cells.Cells were isolated from the inner annulus fibrosus and transition zones of lumbar discs from Sprague-Dawley rats. The cells were grown in monolayer and divided in 5 treatment groups. IL-1beta (10 ng/mL), FasL (5, 20 ng/mL) with/without IL-1beta (10 ng/mL) pre-treatment was respectively added in Dulbeccoos modified Eagleos medium and Hamos F-12 medium with 1% fetal bovine serum. After 32 h, the cells were stained with annexin V-FITC and propidium iodide to evaluate apoptosis using flow cytometry and to analysis transcription of Fas using RT-PCR.Compared with control group, FasL (20 ng/mL), IL-1beta (10 ng/mL)+FasL (5 ng/mL), and IL-1beta (10 ng/mL)+FasL (20 ng/mL) induced significant apoptosis of the disc cells (P0.01). Apoptosis was also induced by FasL 5 ng/mL (P0.05); whereas, apoptosis was not induced by IL-1beta (10 ng/mL) (P0.05). IL-1beta (10 ng/mL) enhanced the apoptosis-inducing effects of FasL (5 ng/mL) and FasL (20 ng/mL) in disc cells. Fas gene transcription in all groups and Fas expression in the 5 treatment groups were approximately 1.2-2.1-fold greater than control group (respectively, P0.05). Additionally, Fas expression in FasL with IL-1beta pre-treatment groups were significantly up-regulated than in FasL groups (P0.01).The results of this study showed disc cells pre-treated with IL-1beta increased apoptotic rate in response to FasL in vitro and provided insights to understand Fas/FasL system-mediated apoptosis in disc cells which would be enhanced due to inflammation factor in degenerative disc.
- Published
- 2007
8. Scoliosis in China
- Author
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Shang-li Liu and Dong-sheng Huang
- Subjects
medicine.medical_specialty ,business.industry ,Incidence (epidemiology) ,Soft tissue ,General Medicine ,Scoliosis ,medicine.disease ,Concave side ,Surgery ,Coronal plane ,medicine ,Vertebrectomy ,Orthopedics and Sports Medicine ,business ,Spinal cord injury ,Mass screening - Abstract
In modern China the investigation and treatment of scoliosis began in the late 1970s. The incidence of scoliosis in the Chinese population of the Sichuan province was 0.064%, and the incidence of >10 degrees for Beijing students was 1.04%. The surgical treatment of scoliosis for 2122 patients reported in the Chinese literature was reviewed by the authors. In this study, the female to male ratio was 1.3:1, the incidence of idiopathic scoliosis was 67.7%, and the incidence of congenital scoliosis was 18.1%. The overall result showed that the correction rate after surgery in the coronal plane was approximately 50% using the Harrington, Luque, Dwyer, or Cotrel-Dubousset instrumentation techniques. However, it would be increased by >60% if a partial vertebrectomy was carefully done and the soft tissue in the concave side was released completely. The postoperative complications were approximately 20% with 0.24% of major spinal cord injury. Biologic and anatomic research of the spine has been done so that the quality of surgical implants can be improved in the future.
- Published
- 1996
9. [Treatment of flatfoot deformity with concurrent spinal disease in children]
- Author
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Wei-dong, Song, De, Li, Shang-li, Liu, Jian-rong, Huang, Hui-yong, Shen, Dong-sheng, Huang, and Ruo-fan, Ma
- Subjects
Male ,Adolescent ,Child, Preschool ,Humans ,Female ,Spinal Diseases ,Child ,Flatfoot ,Retrospective Studies - Abstract
To study the curative effect of operation to flatfoot deformity with concurrent spinal disease in children.A retrospective survey was performed in 8 flatfoot patients with concurrent spinal disease from January 2004 to January 2008. Every case received special operative treatment. After casting, achilles tendon prolongation, reefing or tendon transfer, rehabilitation therapy was performed sequentially. Radiographs and clinical outcomes were evaluated during the follow-up.Six patients were followed up. There were 2 feet in 1 female and 7 feet in 5 males. The complications included tethered cord syndrome (n = 1), T12-S1 vertebral canal arachnoid cyst (n = 1), cerebral palsy and scoliosis (n = 1) and scoliosis and spinal bifida occult (n = 3). The average age was 8.4 (4 - 14) years old and the average follow-up time 22 (14 - 64) months. All cases were satisfied with the appearance and function according to Maryland foot score. Five were excellent, 2 good and 2 fair postoperatively versus 2 fair and 7 failed preoperatively. The total excellent and good rate was 77.8%. In the early stage after tendon transfer in 4 patients, the lateral view of height arch, talus-first metatarsal angle, calcaneus-navicular-first metatarsal angle and talonavicular coverage angle improved. Yet it lost the improved angle and reverted to the pre-operative state. No degenerative change was detected in tarsus joints during the long follow-up.The special and sequential surgical procedure may be an effective regimen in the treatment of flatfoot deformity with concurrent spinal disease in children.
- Published
- 2010
10. Double role of Fas ligand in the apoptosis of intervertebral disc cells in vitro
- Author
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Dunfu, Han, Yue, Ding, Shang-Li, Liu, Gang, Wang, In-Cheong, Si, Xinguang, Wang, Liyang, Cui, and Dongshang, Huang
- Subjects
Male ,Rats, Sprague-Dawley ,Fas Ligand Protein ,Animals ,Apoptosis ,fas Receptor ,In Vitro Techniques ,Intervertebral Disc ,Cells, Cultured ,Intervertebral Disc Displacement ,Rats - Abstract
Fas ligand (FasL) may play an important role in maintaining the immune privilege of intervertebral disc (IVD). Besides, it is closely related to the apoptosis of degenerative disc cells. Nowadays, lots of reports have described about the paradoxical effects of FasL, although the effect of FasL on IVD cells is still under debate. In this study, we tried to investigate the effects of FasL on Fas expression and on the apoptosis of nucleus pulposus (NP) cells in Sprague-Dawley rats. The results showed that the expression of Fas in NP cells was significantly increased by the recombinant FasL. Meanwhile, the apoptosis of NP cells increased markedly in a FasL dose-dependent manner. Interestingly, RNA interference results indicated that the increase of Fas expression and the NP cell apoptosis described previously were inhibited by Fas siRNA, suggesting that RNA interference might be one of novel strategies to prevent IVD cells from apoptosis.
- Published
- 2009
11. [A 10-year-review (1998 - 2007) on 3449 cases of osteoporotic hip fractures: trend of hospitalization and inpatient costs]
- Author
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Zhi-an, Cheng, Ding-kun, Lin, Dong-bin, Liu, Hui-yong, Shen, Qian, Chen, Qi-wen, Qiu, and Shang-li, Liu
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Aged, 80 and over ,Hospitalization ,Male ,Age Distribution ,Hip Fractures ,Humans ,Osteoporosis ,Female ,Health Care Costs ,Length of Stay ,Sex Distribution ,Aged ,Retrospective Studies - Abstract
The purpose of this retrospective research was to analyze the trend of hospitalized prevalence in the past 10 years (1998 - 2007) and to estimate resource utilization associated with osteoporotic hip fractures, including hospital length of stay and inpatient costs in Guangzhou, China.3449 patients aged 50 years and older, hospitalized for primary diagnose of hip fractures in both hospitals in the past ten years were included in our study. Completed data was obtained retrospectively from the hospital medical records. Clinical parameters such as sex-specifics, hospital length of stay, inpatient costs, and the constitutions of the inpatient costs were analyzed with multiple statistics methods and regressions.Over the 10-year study period, the number of hospitalizations for hip fractures increased with a curve model. Male and female ratio was 1:1.95 with an average age of the patients as 76.32 +/- 9.52 years old. Average hospital length of stay was 23.59 +/- 13.48 days with no significant difference between males and females, years of admission and the different diagnoses. The number of hospitalization went to the top at the age of 70 to 79 years old (38.45%), followed by 80-89 years old (33.08%). Femoral neck fractures was significantly more than that of inter-trochanteric fractures in those patients aged 50 to 79 years (P0.01) but less than those intertrochanteric fractures aged 90 years and older (P0.05). Average inpatient costs is about 23.52 +/- 17.00 thousand Yuan (RMB) (femoral neck fractures is about 25.09 +/- 17.62 thousand Yuan and intertrochanteric fracture is 21.44 +/- 15.92 thousand Yuan) of which treatment costs (included implants, materials used in the theatre and routinely care of the wound) contributed 52%, pharmacy intervention 25%, operation 6%, ward expenditure 5%, radiology and physical investigation 5%, and chemistry test 4%. The inpatient cost went to a peak at the age of 60 - 69 years old and were significantly correlated with the hospital length of stay. The cost increased every year in the past 10 years by 6.18%.These results emphasized that the growing economic impact arising from the inpatient treatment of osteoporotic hip fractures and most of which related to treatment cost (include implants, materials used in the theatre and routinely care of the wound), and drugs and always correlated to length of hospital stay. Cost control should be paid more attention to the implants and drugs.
- Published
- 2009
12. The role of venous hypertension in the pathogenesis of Legg-Perthes disease. A clinical and experimental study
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Tien-Chi Ho and Shang-Li Liu
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medicine.medical_specialty ,medicine.diagnostic_test ,Aseptic necrosis ,business.industry ,Venography ,Avascular necrosis ,General Medicine ,medicine.disease ,Surgery ,Pathogenesis ,Femoral head ,medicine.anatomical_structure ,medicine ,Orthopedics and Sports Medicine ,Venous hypertension ,business ,Femoral neck ,Legg-Perthes Disease - Abstract
Thirty-two patients in whom Legg-Perthes disease apparently involved only one hip were examined with venography, measurement of intraosseous and intra-articular pressures, arthrography, and dynamic triphasic bone-imaging with 99mTc methylene diphosphonate. The arterial flow of blood in the affected femoral head was slightly decreased, but the difference from that on the normal side was not statistically significant. However, there was marked disturbance of the venous drainage in the diseased hip, elevated intraosseous pressure in the affected femoral neck, and increased intra-articular pressure in the involved hip compared with the normal side. An animal model was then created in twenty immature dogs, venous drainage was obstructed, and intraosseous pressure of the femoral head and neck was elevated by injection of four milliliters of semiliquid silicone into the femoral neck. In eleven of the dogs, areas of avascular necrosis resembling those associated with Legg-Perthes disease developed in the femoral head.
- Published
- 1991
13. [Association of 86 bp variable number tandem repeat polymorphism of interleukin-1 receptor antagonist gene with lumbar disc disease]
- Author
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Wei, Ye, Dong-Sheng, Huang, Wei-Jian, Chen, Chun-Hai, Li, Yan, Peng, An-Jing, Liang, and Shang-Li, Liu
- Subjects
Adult ,Male ,Polymorphism, Genetic ,Intervertebral Disc Degeneration ,Minisatellite Repeats ,Middle Aged ,Interleukin 1 Receptor Antagonist Protein ,Young Adult ,Asian People ,Case-Control Studies ,Humans ,Female ,Spinal Diseases ,Genome-Wide Association Study - Abstract
To investigate the association of 86 bp variable number tandem repeat (VNTR) polymorphism of interleukin-1 receptor antagonist (IL-1Ra) gene with lumbar disc disease and intervertebral disc degeneration.The 86 bp VNTR polymorphism of IL-1Ra gene was analyzed with PCR and electrophoresis for 81 patients with lumbar disc disease and 101 volunteers without sciatica (control). The grade of intervertebral disc degeneration was determined with magnetic resonance imaging, and the association of 86 bp VNTR polymorphisms with lumbar disc disease and intervertebral disc degeneration in those younger than 45 years was assessed.The presence of 86bp VNTR polymorphisms of IL-1Ra gene was detected in both patients with lumbar disc disease and the control subjects. The distribution of 86 bp VNTR polymorphisms of IL-1Ra gene showed no significant difference between the two groups, but the distributions of 1/1, 1/2 and 2/2 or 1, 2 genotypes differed significantly. The current data did not support a significant association between the distribution of IL-1Ra gene 86bp VNTR polymorphism and lumbar disc degeneration.IL-1Ra gene 86bp VNTR polymorphism is present among Chinese population in association with lumbar disc disease, but not with lumbar disc degeneration.
- Published
- 2007
14. [Effect of interleukin-6 on the chondrocytes in the cartilage endplate of rabbits in vitro]
- Author
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Wei, Ye, Ruo-Fan, Ma, Yue, Ding, Dong-Sheng, Huang, Wei-Jian, Chen, Yan, Peng, and Shang-Li, Liu
- Subjects
Male ,Cartilage ,Chondrocytes ,Gene Expression Regulation ,Interleukin-6 ,Cell Cycle ,Animals ,Female ,Aggrecans ,RNA, Messenger ,Rabbits ,Collagen Type II ,Cell Proliferation - Abstract
To evaluate the effect of interleukin-6 (IL-6) on the biological behaviors of the chondrocytes in the cartilage endplate of rabbits.Chondrocytes isolated from the cartilage endplate of New Zealand rabbits, verified for their biological characteristics by such means as toluidine blue staining for type II collagen, were treated with IL-6 at different concentrations. The proliferation of the chondrocytes was evaluated by MTT assay at different time points following the treatment, the cell cycle changes were determined by flow cytometry and the changes of aggrecan and type II collagen mRNAs detected by RT-PCR.At the concentrations of 10, 50 and 100 ng/ml, IL-6 did not obviously affect the rate of chondrocyte proliferation. IL-6 at 50 ng/ml resulted in no obvious changes of the cell cycle of the chondrocytes, but significantly decreased the expression of collagen IIa mRNA.IL-6 has no effect on the proliferation and cell cycle of the chondrocytes, but at higher concentrations, it inhibits matrix synthesis of the chondrocytes to promote intervertebral disc degeneration.
- Published
- 2007
15. [Stability of lumbar spine after artificial disc replacement]
- Author
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Guang-hua, Lei, Kang-hua, Li, Shang-li, Liu, Fei-yue, Lin, Liang-jun, Li, and Xian-cun, Song
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Adult ,Joint Instability ,Male ,Prosthesis Implantation ,Lumbar Vertebrae ,Cadaver ,Humans ,Female ,Intervertebral Disc - Abstract
To determine the influence of artificial disc replacement on the stability of lumbar.Ten fresh human cadaveric lumbar spine specimens (9 males, 1 female) were tested. The donors had a mean age of 29.3 years old. The segments were fixed on a special testing device mounted in a universal testing machine. Physiological load with a maximum of 10 Nm was applied in the flexion, extension, right and left side bending. Moments were applied in 6 load steps: 0, 2, 4, 6, 8, and 10 Nm. Linearity migration and angle migration were recorded,and the mean flexibility coefficients were calculated. Four procedures of measurement were performed in every specimen: intact segments, segments after discectomy, segments after the artificial disc replacement, and segments after the artificial disc replacement with cutting posterior longitudinal ligament locally.The indexes we calculated of the segments after the discectomy were statistically different compared with the intact segments (P0.05, P0.01). The linearity migration,angle migration and mean flexibility coefficient in flexion/extension direction of the segments after the artificial disc replacement were statistically different compared with the segments after the discectomy (P0.05, P0.01), and there was a statistically significant increase in angle migration and mean flexibility coefficient in the extension direction compared with the intact situation (P0.05). There was no statistically significant difference in 4 directions between the segments after the artificial disc replacement and the segments after the artificial disc replacement with cutting posterior longitudinal ligament locally (P0.05).Artificial intervertebral disc replacement could keep the stability of the lumbar functional spine unit. There was no harmful effect of cutting posterior longitudinal ligament locally on the stability of lumbar while performing artificial intervertebral disc replacement when it is necessary.
- Published
- 2006
16. [The risk factors and preventive strategies of heterotopic ossification after artificial disc replacement in lumbar spine]
- Author
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Dong-sheng, Huang, An-jing, Liang, Wei, Ye, Pei-qiang, Su, Shang-li, Liu, Ruo-fan, Ma, Bi-ling, Liang, and Guo-zhao, Li
- Subjects
Adult ,Male ,Prosthesis Implantation ,Lumbar Vertebrae ,Risk Factors ,Ossification, Heterotopic ,Humans ,Female ,Middle Aged ,Aged ,Diskectomy ,Follow-Up Studies ,Retrospective Studies - Abstract
To analyze the risk factors of heterotopic (HO) ossification after total lumbar disc replacement and probe the preventive strategies for it.The radiographs and clinical data of 78 discs in 65 patients who received artificial lumbar disc replacement (ADR) from April 1998 to December 2003 were analyzed retrospectively by two radiologists and one orthopaedic surgeon and then postoperative HO were graded according to McAfee system. The bony formations in disc spaces, time of HO were found, and range of motion (ROM) of the operated levels were measured on radiographic films. In addition, the risk factors such as preoperative peri-annulus ossification, bony endplates injuries, and subsided or mal-position of the prostheses were also analyzed by Logistic regression analysis.Postoperative HO was found in 10 spaces of 9 cases. Class I of HO were occurred in 7 patients at means 2.1 years postoperatively with normal range of motion preserved. Three of them turned into class II or III with 10 degrees of mean ROM in the following 2.5 years. Another 2 (2/9) cases with preoperative peri-annulus ossification had bridging trabecular bone (class III) between the endplates and 9 degrees of ROM 2 years after surgery, then turned into class IV at 6 years with 0 degrees and 4 degrees of motion in the operated levels. As the risk factors of HO, preoperative annulus ossification (2 cases), bony endplates injuries (5 cases), mal-positioned prostheses (2 cases) and subsided prostheses (2 cases) were found simultaneity with significant positive relation to HO occurred (P0.05).Factors such as preoperative ossification of annulus, endplate injuries, prosthesis subsided and mal-position would have higher risks to have HO occurred after ADR, but ROM of most affected levels are preserved. Strict control indication and avoid all above risk factors can prevent HO occurring effectively.
- Published
- 2006
17. [The application of METRx microendoscopes diskectomy system in the treatment of far lateral lumbar disc herniation]
- Author
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Chun-hai, Li, Shang-li, Liu, Dong-sheng, Huang, Yue, Ding, and Jie-min, He
- Subjects
Adult ,Male ,Microsurgery ,Lumbar Vertebrae ,Treatment Outcome ,Humans ,Endoscopy ,Female ,Middle Aged ,Intervertebral Disc Displacement ,Diskectomy ,Follow-Up Studies - Abstract
To introduce the METRx microendoscopes diskectomy system in the treatment of far lateral lumbar disc herniation.Fourteen cases of far lateral lumbar disc herniation were operated with METRx from February 1999 to December 2002. Among them, the average age was 49 years old (range 41 - 55 years old), male in 10 cases, female in 4 cases. All cases were single disc herniation; L(4), 5 herniation in 6 discs, L(5)-S(1) herniation in 8 discs; foraminal disc herniation in 6 cases, extra-foraminal disc herniation in 8 cases.All the cases were followed up from 12 to 46 months (average 26.5 months) with the results of excellence in 10 cases, good in 3 cases, fair in 1 case and no failure case. There were no disc infection, dura laceration, nerve root injury and herniation recurrence.METRx is suitable for far lateral lumbar disc herniation with the advantages of minimal invasive, complete decompression of nerve root and rapid recovery. The correct approach and precise surgical technique are the key points for this operation.
- Published
- 2006
18. [Design and experiment of the pedicle aiming device for the cervical spine]
- Author
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Dong-sheng, Huang, Pei-qiang, Su, Ruo-fan, Ma, Chun-hai, Li, Yan, Peng, and Shang-li, Liu
- Subjects
Adult ,Male ,Stereotaxic Techniques ,Spinal Fusion ,Therapy, Computer-Assisted ,Bone Screws ,Cervical Vertebrae ,Humans ,Female ,Tomography, X-Ray Computed ,Internal Fixators ,Orthopedic Fixation Devices - Abstract
To probe the accuracy and safety of using an aiming device in the transpedicular fixation of cervical spine.Eight cervical specimens were obtained. We used the computed tomography (CT) to scan C3 to C7, measured the medial angle of the pedicle, and determined the location of the pedicle projecting on the articular process. Then we took the oblique X-ray film, measured the cephalic/caudal angle of the pedicle, and determined the location of the pedicle projecting on the articular process. All the specimens were equally divided into two groups. Screws of 2.8 mm x 30 mm, were used. Specimens in one group were inserted with the transpedicular screw manually, while specimens in the other one inserted with the transpedicular screw using a self-designed aiming device that can be modulate at the three dimensions according to the angles of the pedicles.The first group totally had 40 screws from C3 to C7. There were 13 screws in the pedicle, 9 violated the walls of the pedicle but not involved the adjunct structure, and 18 injured the important structure such as spinal cord, verteberal artery, or nerve root. In the other group, only 4 screws violated the walls of the pedicle but not involved the adjunct structure, and the others all in the pedicles. The difference was of statistical significance (P0.01).In the cervical spine, transpedicular fixation using an aiming device can improve the accuracy and safety during operation.
- Published
- 2005
19. [The role of artificial disc replacement in restoring lumbar segment motion and intervertebral height]
- Author
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Shang-Li, Liu, Dong-Sheng, Huang, An-Jing, Liang, Wei, Ye, Chun-Hai, Li, and Ruo-Fan, Ma
- Subjects
Adult ,Male ,Lumbar Vertebrae ,Joint Prosthesis ,Prostheses and Implants ,Recovery of Function ,Middle Aged ,Spinal Fusion ,Humans ,Female ,Range of Motion, Articular ,Intervertebral Disc ,Intervertebral Disc Displacement ,Diskectomy ,Follow-Up Studies - Abstract
To investigate the role of artificial disc replacement (ADR) in restoring lumbar segment motion and intervertebral height (IH).Seventy-five patients with intervertebral disc diseases underwent artificial disc replacement and were divided into 3 groups to be followed up for 2 years (group A, n = 32), 3 years (group B, n = 27), and 5 years (group C, n = 19) respectively. Before the operation and during the follow-up the range of motion (ROM) of the involved lumbar segments and IH were assessed.The mean ROM and IH of the group A were 15 degrees and 14.1 mm respectively during the follow-up 2 years after operation. The mean ROM and IH of the group B were 14.2 degrees and 13.8 mm respectively during the follow-up 2 years after the operation and 13.7 degrees and 13.4 mm respectively during the follow-up 3 years after operation. The mean ROM and IH of the group C were 9.1 degrees and 11.5 mm respectively during the follow-up 2 years after the operation and 8.8 degrees and 11.0 mm respectively during the follow-up 3 years after operation, and 8.0 degrees and 10.3 mm respectively during the follow-up 5 years after operation. No significant differences in ROM and IH were found among different groups at any time point (all P0.05). Malposition was found in immediately after operation in 11 patients, all of the group C. During the follow-up, 3 cases of prostheses subsidence and 3 cases of annular ossification were found. No severe complications appeared.ADR is effective in restoring the ROM and IH of vertebrae suffering from intervertebral disc diseases.
- Published
- 2005
20. [Comparative study of vertebral body stress distribution following insertion of artificial lumbar intervertebral disc]
- Author
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Yi-chun, Xu, Shang-li, Liu, Mei-chao, Zhang, Dong-sheng, Huang, and Qi-you, Wang
- Subjects
Prosthesis Implantation ,Lumbar Vertebrae ,Finite Element Analysis ,Humans ,Prostheses and Implants ,Stress, Mechanical ,Intervertebral Disc ,Models, Biological ,Diskectomy - Abstract
To study vertebral body stress distribution of normal disc, post-Diskectomy and artificial disk respectively by 3-D finite element methods, and to explore artificial intervertebral disk insertion impact on stress distribution of vertebral body.Models of normal disk, post-Diskectomy, artificial disk and L(4 - 5) motion segment were established by using finite element software MSC. MARK, then vertebral body stress was analyzed through model of L(4 - 5) motion segment respectively.The vertebral body's stress was the smallest after insertion of artificial intervertebral disk (AID), and its stress distributed equally. But the stress under post-discectomy was bigger than the normal disc's in all the motion state. On the other hand, the stress distribution state of the post-discectomy changed while the spine were in different motion state, during the spine flexion, the stress in the anterior of vertebral body was the biggest; While extension, in the posterior and in right flexion state, the biggest stress was in the right. While vertical compression and rotation, the stress distributed equally.The results illustrate that the vertebral body's stress is the smallest after insertion of AID in 3 groups of all motion state, and its stress distributes equally. But the level of vertebral body stress increases after discectomy comparing with the normal group. In generally, it is much more reasonable that the disc is reconstructed with AID because of the biomechanical effect on vertebral body made by AID insertion.
- Published
- 2005
21. [Stimulation of insulin-like growth factor-I to chondrogenesis of engineering cartilage tissue]
- Author
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Jian-rong, Huang, Shang-li, Liu, and Wei-dong, Song
- Subjects
Cartilage, Articular ,Chondrocytes ,Tissue Engineering ,Polymers ,Humans ,Insulin-Like Growth Factor I ,Chondrogenesis ,Collagen Type II ,Immunohistochemistry ,Cell Division ,Cells, Cultured - Abstract
To explore the ability of insulin-like growth factor-I (IGF-I) and hyaluracan acid in prompting chondrogenesis of engineering cartilage tissue.Human articular chondrocytes were isolated and cultured in DMEM plus 10% fetal bovine serum. They were divided into three groups: hyaluracan acid + chondrocytes + IGF-I group (IGF-I group), hyaluracan acid + chondrocytes group (cell group), hyaluracan acid group (control group). The ability of chondrogenesis was investigated by HE and toluidine blue staining, human collagen II immunohistochemistry and reverse transcription polymerase chain reaction (RT-PCR).Both cell group and IGF-I group could develop into cartilage tissue in the sixth week while control group could not. The number of cartilage lacuna in IGF-I group were more than that in cell group. Human collagen II immunohistochemistry showed that there were stronger positive cell in IGF-I group than in cell group, collagen II mRNA expression was more higher and collagen I mRNA expression was lower in IGF-I group than in cell group.Insulin growth factor-I can prompt chondrogenesis of engineering cartilage tissue and ameliorate the quality of engineering cartilage tissue in vitro.
- Published
- 2004
22. Clinical study on periprosthetic bone loss after THA
- Author
-
Ruo-fan Ma, Hai Qi, Yue Ding, Hui-yong Shen, Shang-li Liu, and Zhi-ping Guan
- Subjects
Clinical study ,medicine.medical_specialty ,Histology ,Physiology ,business.industry ,Endocrinology, Diabetes and Metabolism ,Periprosthetic ,Medicine ,business ,Surgery - Published
- 2010
23. Fusion of scoliosis by Harrington distraction rod. Interspinous process and sublaminar wiring compared in 42 cases
- Author
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Shang-Li Liu, F. William Schroeder, Tomasz Borowiecki, and Scott S. Russo
- Subjects
Adult ,Male ,medicine.medical_specialty ,Adolescent ,Chirurgie orthopedique ,Arthrodesis ,medicine.medical_treatment ,education ,Scoliosis ,Distraction ,medicine ,Humans ,Orthopedics and Sports Medicine ,Early Ambulation ,Braces ,business.industry ,medicine.disease ,humanities ,Internal Fixators ,Surgery ,body regions ,Radiography ,Spinal Fusion ,Orthopedic surgery ,Female ,business ,Bone Wires ,Follow-Up Studies - Abstract
Forty-two consecutive patients with scoliosis who between 1981 and 1988 underwent posterior Harrington distraction rod and interspinous process or sublaminar wiring were retrospectively reviewed. No difference was found between the techniques with respect to age, sex, curve pattern, curve magnitude, levels fused, operative time, blood loss, correction, and loss of correction at the 1-year and 2-year follow-up. The interspinous process wiring was superior to the sublaminar wiring as regards ease of technique, early ambulation, few complications, and a more effective means for maintaining the correction without postoperative immobilization.
- Published
- 1991
24. A comparative study of Toxoplasma gondii seroprevalence in mink using a modified agglutination test, a Western blot, and enzyme-linked immunosorbent assays.
- Author
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Gu Y, Wang Z, Cai Y, Li X, Wei F, Shang L, Li J, and Liu Q
- Subjects
- Agglutination Tests veterinary, Animals, Antibodies, Protozoan blood, Blotting, Western veterinary, China epidemiology, Enzyme-Linked Immunosorbent Assay veterinary, Prevalence, ROC Curve, Sensitivity and Specificity, Seroepidemiologic Studies, Toxoplasma immunology, Toxoplasmosis, Animal blood, Toxoplasmosis, Animal diagnosis, Mink, Toxoplasma isolation & purification, Toxoplasmosis, Animal epidemiology
- Abstract
Toxoplasma gondii can infect almost all warm-blooded animals, and many serological methods have been developed to detect T. gondii infection in a variety of animal species. In the present study, the seroprevalence of T. gondii infection in farmed mink in northeast China was determined using the modified agglutination test (MAT), a Western blot (WB), and 3 enzyme-linked immunosorbent assays (ELISAs) with protein A/G conjugate, using either of 2 recombinant dense granule antigens, GRA1 and GRA7, or Toxoplasma soluble antigens (TSA). There was no significant difference between the detection results of the GRA1-, GRA7-, and TSA-ELISAs and WB (McNemar chi-square, P > 0.05), but a significant difference was observed between MAT and WB (P < 0.05). A near perfect agreement (97.0%) was found between the GRA7-ELISA and WB (κ = 0.83), and a substantial agreement (92.4-93.1%) was observed in the TSA- and GRA1-ELISAs (κ = 0.68-0.73). The GRA7-ELISA showed the highest sensitivity and specificity, and the lowest false-positive and negative rates, while the MAT gave both a low sensitivity and frequent false positives in comparison to the WB. Receiver operating characteristic analysis revealed the largest area under curve of 0.85 (95% confidence interval: 0.74-0.96), and the highest relative sensitivity (72.7%) and specificity (99.0%) for a cutoff value of 0.19 in the GRA7-ELISA. These results indicate that the GRA7-ELISA is suitable for detection of T. gondii infection in mink and that MAT should be used with caution., (© 2015 The Author(s).)
- Published
- 2015
- Full Text
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