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165 results on '"Shang, Zhenhua"'

15. LncRNA RNF144A-AS1 Promotes Bladder Cancer Progression via RNF144A-AS1/miR-455-5p/SOX11 Axis

Author

Bi, Huifeng, Shang, Zhenhua, Jia, Chunsong, Wu, Jiangtao, Cui, Bo, Wang, Qi, and Ou, Tongwen

Subjects
SOX11 gene, lncRNA RNF144A-AS1, miRNA-455-5p, bladder cancer, OncoTargets and Therapy, Original Research, bladder cancer progression
Abstract

Huifeng Bi,1,2 Zhenhua Shang,1 Chunsong Jia,1 Jiangtao Wu,1 Bo Cui,1 Qi Wang,1 Tongwen Ou1 1Department of Urology, Xuanwu Hospital Capital Medical University, Beijing, People’s Republic of China; 2Department of Urology, Jincheng General Hospital, Jincheng, Shanxi Province, People’s Republic of ChinaCorrespondence: Tongwen OuDepartment of Urology, Xuanwu Hospital Capital Medical University, No. 45 Changchun Street, Xicheng District, Beijing 100053, People’s Republic of ChinaTel +86 135 0106 5134Fax +86 010 8319 8388Email outongwen1967@126.comBackground: Bladder cancer (BC) is the most commonly occurring malignant tumor of the urinary system worldwide. Long non-coding RNAs (lncRNAs), including lncRNA RNF144A-AS1 (RNF144A-AS1), perform an oncogenic role in BC progression. However, how RNF144A-AS1 is regulated in BC has not been fully investigated, and its role in BC is mostly obscure. In this study, we explore its role in BC progression.Materials and Methods: The expression level of RNF144A-AS1 in BC tissues was explored via bioinformatics analysis and quantitative real-time PCR (qRT-PCR). We used RNF144A-AS1 siRNA (si-RNF144A-AS1) to inhibit the RNF144A-AS1 level in BC cell lines (J82 and 5637 cells). A series of experimental studies in vitro (CCK-8 assay, colony formation assay and Transwell assay) was performed to explore the role of si-RNF144A-AS1 on the proliferation, migration and invasion of J82 and 5637 cells. A BC xenograft model was established, and the effect of si-RNF144A-AS1 on xenograft growth was explored in vivo. The interactions among RNF144A-AS1, miR-455-5p and SOX11 were predicted by bioinformatics miRanda and Targetscan database, and verified by the luciferase reporter assay and RNA pull-down assay. Finally, miR-455-5p inhibitor and si-RNF144A-AS1 were cotransfected into J82 and 5637 cells.Results: RNF144A-AS1 is overexpressed in BC tumors and cells, and its overexpression is correlated with poor prognosis. Knockdown of RNF144A-AS1 markedly suppressed the proliferation, migration and invasion of J82 and 5637 cells and significantly inhibited xenograft growth in nude mice, compared to si-NC. We found that RNF144A-AS1 serves as a sponge for miR-455-5p. Furthermore, a binding site of miR-455-5p was found in 3ʹ UTR of SOX11 gene, and overexpression of miR-455-5p suppressed SOX11 levels. RNF144A-AS1 knockdown markedly decreased SOX11 expression levels, while miR-455-5p inhibitor restored this repressive effect. Restoration of SOX11 could reverse this repressive effect of RNF144A-AS1 on cell proliferation, migration and invasion abilities.Conclusion: Overall, our findings underline the critical role of RNF144A-AS1 in BC development, and our study reveals for the first time that RNF144A-AS1 promotes BC progression via the RNF144A-AS1/miR-455-5p/SOX11 axis.Keywords: bladder cancer, lncRNA RNF144A-AS1, miRNA-455-5p, SOX11 gene, bladder cancer progression

Published
2020

29. A Bacterial Type III Effector Targets the Master Regulator of Salicylic Acid Signaling NPR1 to Subvert Plant Immunity

Author

Chen, Huan, primary, Chen, Jian, additional, Li, Min, additional, Chang, Ming, additional, Xu, Kaimei, additional, Shang, Zhenhua, additional, Zhao, Yi, additional, Palmer, Ian, additional, Zhang, Yuqiang, additional, McGill, Jon, additional, Alfano, James R., additional, Nishimura, Marc T., additional, Liu, Fengquan, additional, and Fu, Zheng Qing, additional

Published
2018
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30. A Bacterial Type III Effector Targets the Master Regulator of Salicylic Acid Signaling, NPR1, to Subvert Plant Immunity

Author

Chen, Huan, primary, Chen, Jian, additional, Li, Min, additional, Chang, Ming, additional, Xu, Kaimei, additional, Shang, Zhenhua, additional, Zhao, Yi, additional, Palmer, Ian, additional, Zhang, Yuqiang, additional, McGill, Jon, additional, Alfano, James R., additional, Nishimura, Marc T., additional, Liu, Fengquan, additional, and Fu, Zheng Qing, additional

Published
2017
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32. Fluorescence imaging of dendritic spines of Golgi-Cox-stained neurons using brightening background

Author

Muqing Chen, Xiuli Liu, Tao Yang, Shaoqun Zeng, Min Ai, Shang Zhenhua, and Hanqing Xiong

Subjects
Background fluorescence, Male, Pathology, medicine.medical_specialty, Fluorescence-lifetime imaging microscopy, Dendritic spine, Confocal, Dendritic Spines, Biomedical Engineering, law.invention, Biomaterials, Mice, Confocal microscopy, law, Microscopy, Fluorescence microscope, medicine, Animals, Fluorescent Dyes, Microscopy, Confocal, Chemistry, Pyramidal Cells, Golgi cox, Atomic and Molecular Physics, and Optics, Electronic, Optical and Magnetic Materials, Microscopy, Fluorescence, Biophysics
Abstract

We report a novel fluorescence imaging approach to imaging nonfluorescence-labeled biological tissue samples. The method was demonstrated by imaging neurons in Golgi-Cox-stained and epoxy-resin-embedded samples through the excitation of the background fluorescence of the specimens. The dark neurons stood out clearly against background fluorescence in the images, enabling the tracing of a single dendritic spine using both confocal and wide-field fluorescence microscopy. The results suggest that the reported fluorescence imaging method would provide an effective alternative solution to image nonfluorescence-labeled samples, and it allows tracing the dendritic spine structure of neurons.

Published
2014

33. Injecting RNA interference lentiviruses targeting the muscarinic 3 receptor gene into the bladder wall inhibits neurogenic detrusor overactivity in rats with spinal cord injury.

Author

Shang, Zhenhua, Jia, Chunsong, Yan, Hao, Cui, Bo, Wu, Jiangtao, Wang, Qi, Gao, Wei, Cui, Xin, Li, Jin, and Ou, Tongwen

Abstract

Aims: To investigate the effects of injecting RNA interference (RNAi) lentiviruses targeting the muscarinic 3 (M3) receptor gene into the bladder wall on bladder activity in rats with spinal cord injury (SCI). Methods: Four M3 RNAi lentiviruses were constructed and used to infect primary cultured bladder smooth muscle cells (BSMCs). Western blotting and quantitative reverse transcription polymerase chain reaction (qRT‐PCR) were performed to determine the optimal RNAi lentivirus with the highest interference efficiency. Female Wistar rats were subjected spinal cord transection at T9‐10 and randomly divided into three groups (n = 8), namely, blank control, negative control, and experimental groups, and injected into the bladder wall with saline, negative control shRNA, and M3 RNAi lentiviruses, respectively, 1 week after spinal cord transection. The normal rats were used as normal control group. Urodynamic parameters and bladder tissues were evaluated in the different groups. Results: An M3 RNAi lentivirus with the highest interference efficiency (78.9%) was constructed and identified. Three weeks after injecting M3 RNAi lentiviruses into the bladder wall, Western blotting and qRT‐PCR showed that the M3 receptor was significantly downregulated in the experimental group. Cystometric evaluation suggested that downregulating M3 receptor expression could substantially decrease basal pressure, residual volume, and non‐voiding contraction number, increase intercontraction interval, and significantly improve bladder compliance in rats with SCI. Conclusion: Injecting RNAi lentiviruses targeting the M3 receptor gene into the bladder wall could effectively inhibit neurogenic detrusor overactivity (NDO) due to SCI. Thus, this approach may be a potential treatment for NDO in SCI. [ABSTRACT FROM AUTHOR]

Published
2019
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43. Rapid imaging of large tissues using high-resolution stage-scanning microscopy

Author

Ting Zheng, Jing Yuan, Xiaohua Lv, Shang Zhenhua, Xiaojun Wang, Shaoqun Zeng, and Tao Yang

Subjects
Fluorescence-lifetime imaging microscopy, Materials science, Optical sectioning, Pixel, business.industry, Detector, Scanning confocal electron microscopy, Article, Atomic and Molecular Physics, and Optics, law.invention, Lens (optics), Optics, law, Confocal microscopy, Microscopy, business, Biotechnology
Abstract

Rapid and high-resolution imaging of large tissues is essential in biological research, like brain neuron connectivity research and cancer margins imaging. Here a novel stage-scanning confocal microscopy was developed for rapid imaging of large tissues. Line scanning methods and strip imaging strategy were used to increase the imaging speed. The scientific CMOS was used as line detector in sub-array mode and the optical sectioning ability can be easily adjusted by changing the number of line detectors according to different samples. Fluorescent beads imaging showed resolutions of 0.47 μm, 0.56 μm, and 1.56 μm in the X, Y, and Z directions, respectively, with a 40 × objective lens. A 10 × 10 mm(2) coronal plane with enough signal intensity could be imaged in about 88 sec at a sampling resolution of 0.16 μm/pixel. Rapid imaging of mouse brain slices demonstrated the applicability of this system in visualizing neuronal details at high frame rate.

Published
2015

48. An uncommon manifestation of paraneoplastic cerebellar degeneration in a patient with high grade urothelial, carcinoma with squamous differentiation: A case report and literature review.

Author

Yaofeng Zhu, Shouzhen Chen, Songyu Chen, Jing Song, Fan Chen, Hu Guo, Zhenhua Shang, Yong Wang, Changkuo Zhou, Benkang Shi, Zhu, Yaofeng, Chen, Shouzhen, Chen, Songyu, Song, Jing, Chen, Fan, Guo, Hu, Shang, Zhenhua, Wang, Yong, Zhou, Changkuo, and Shi, Benkang

Subjects
PARANEOPLASTIC syndromes, CEREBELLUM degeneration, BLADDER cancer treatment, TRANSITIONAL cell carcinoma, SQUAMOUS cell carcinoma, CANCER treatment, TRANSURETHRAL prostatectomy, GLUCOCORTICOIDS, THERAPEUTICS, CANCER relapse, URINARY organs, TREATMENT effectiveness, PAPILLARY carcinoma, CYSTECTOMY, DIAGNOSIS, TUMORS
Abstract

Background: Paraneoplastic neurological syndromes (PNS) are rare disorders associated with malignant tumours, which are triggered by autoimmune reactions. Paraneoplastic cerebellar degeneration (PCD) is the PNS type most commonly associated with ovarian and breast cancer. Two bladder cancers manifesting in PCD were previously reported. However, the cancers in these cases had poor outcomes.Case Presentation: Here, we present a 68-year old man with history of high-grade papillary urothelial carcinoma of the bladder. The patient suffered from persistent cerebellar ataxia accompanied by bladder cancer recurrence five months after transurethral resection of the bladder tumour (TURBt). Laboratory screening for the specific antibodies of paraneoplastic neurological syndromes revealed no positive results. Symptoms were not remitted after a 7-day-course of high-dose glucocorticoid therapy. To our surprise, the patient recovered fully after laparoscopic radical cystectomy. Postoperative pathology revealed that surgical specimens were urothelial carcinoma in situ (CIS) and squamous cell carcinoma of the bladder. The patient remained asymptomatic and there was no evidence of recurrence after the followup period of 11 months.Conclusion: To our knowledge, this is the third report of PCD in a patient with bladder cancer. This case showed that tumour resection cured the PCD. To assist clinical evaluation and management, literature regarding basic PNS characteristics and bladder cancers was reviewed. [ABSTRACT FROM AUTHOR]

Published
2016
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