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240 results on '"Shang, Ming-Ying"'

2. 20 potentially new compounds and 11 new bioactive constituents found in Smilacis Glabrae Rhizoma utilizing HPLC‐DAD‐ESI‐IT‐TOF‐MSn.

Author

Gegentana, Xu, Feng, Huang, Yan‐Fei, Li, Hong‐Fu, Yang, Ping, Shang, Ming‐Ying, Liu, Guang‐Xue, Li, Yao‐Li, Wang, Xuan, and Cai, Shao‐Qing

Abstract

Introduction: Smilacis Glabrae Rhizoma (SGR) is rich in chemical constituents with a variety of pharmacological activities. However, in‐depth research has yet to be conducted on the chemical and pharmacodynamic constituents of SGR. Materials and methods: In this study, the chemical constituents of SGR were analyzed using liquid chromatography–mass spectrometry, and the pharmacodynamic compounds responsible for the medicinal effects of SGR were elucidated through a literature review. Results: In total, 20 potentially new compounds, including 16 flavonoids (C19, C20, and C27–C40) and four phenylpropanoids (C107, C112, C113, and C118), together with 161 known ones were identified in the ethanol extract of SGR using liquid chromatography–mass spectrometry, and 25 of them were unequivocally identified by comparison with reference compounds. Moreover, 17 known constituents of them were identified in the plants of genus Smilax for the first time, and 16 were identified in the plant Smilax glabra Roxb. for the first time. Of 161 known compounds, 84 constituents (including isomers) have been reported to have 17 types of pharmacological activities, covering all known pharmacological activities of SGR; among these 84 bioactive constituents, six were found in the plants of genus Smilax for the first time and five were found in S. glabra for the first time, which are new bioactive constituents found in the plants of genus Smilax and the plant S. glabra, respectively. Conclusion: The results provide further information on the chemical composition of SGR, laying the foundation for the elucidation of the pharmacodynamic substances of SGR. The chemical constituents of Smilacis Glabrae Rhizoma (SGR) were analyzed using liquid chromatography–mass spectrometry, and the pharmacodynamic compounds responsible for the medicinal effects of SGR were elucidated through a literature review. A total of 181 chemical constituents were identified in the ethanol extract of SGR, including 20 potentially new compounds, 17 first‐discovered constituents (containing six bioactive constituents) in the plants of genus Smilax, and 16 first‐discovered constituents (containing five bioactive constituents) in the plant of Smilax glabra Roxb. [ABSTRACT FROM AUTHOR]

Published
2024
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18. The profiling and identification of the absorbed constituents and metabolites of Paeoniae Radix Rubra decoction in rat plasma and urine by the HPLC–DAD–ESI-IT-TOF-MSn technique: A novel strategy for the systematic screening and identification of absorbed constituents and metabolites from traditional Chinese medicines

Author

Liang, Jing, Xu, Feng, Zhang, Ya-Zhou, Huang, Shuai, Zang, Xin-Yu, Zhao, Xin, Zhang, Lei, Shang, Ming-Ying, Yang, Dong-Hui, Wang, Xuan, and Cai, Shao-Qing

Published
2013
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28. Production, characterization of a monoclonal antibody against aristolochic acid-II and development of its assay system

Author

Tian, Min, Tanaka, Hiroyuki, Shang, Ming-Ying, Karashima, Seiko, Chao, Zhi, Wang, Xuan, Cai, Shao-Qing, and Shoyama, Yukihiro

Subjects
Serum albumin -- Research, Serum albumin -- Physiological aspects, Monoclonal antibodies -- Research, Monoclonal antibodies -- Physiological aspects, Animal models in research -- Usage, Enzyme-linked immunosorbent assay -- Usage, Aristolochiaceae -- Usage, Aristolochiaceae -- Health aspects, Aristolochiaceae -- Research, Health
Published
2008

36. Global Profiling and Structural Characterization of Metabolites of Ononin Using HPLC-ESI-IT-TOF-MSnAfter Oral Administration to Rats

Author

Li, Hong-Fu, Li, Teng, Yang, Ping, Wang, Yong, Tang, Xue-Jian, Liu, Li-Jia, Xu, Feng, Shang, Ming-Ying, Liu, Guang-Xue, Li, Yao-Li, Wang, Xuan, Yin, Jun, and Cai, Shao-Qing

Abstract

Ononin is a bioactive isoflavone of legumes. To explore the “effective forms” of ononin, its metabolites were characterized using HPLC–ESI-IT-TOF-MSnafter oral administration to rats. Metabolites (106), including 94 new metabolites, were characterized, which contained 17 phase I, 23 hydroxylated and methylated, 54 sulfated, 10 glucuronidated, and 2 sulfated and glucuronidated metabolites. Six hydroxylated metabolites of formononetin (aglycone of ononin) were simultaneously detected for the first time. Twenty-three hydroxylated and methylated metabolites were the new metabolites of ononin, and the number of hydroxylation and methylation was 1–3 and 1–2. Twenty metabolites have ononin-related bioactivities, and many metabolites have the same bioactivities. Their probable mechanisms of action may be additive and/or synergistic effects, especially because of the addition of the blood concentrations of these compounds. The results provide a foundation for a better understanding of the “effective forms” of ononin.

Published
2020
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47. Investigation of the in vivo metabolism of harpagoside and distribution of its metabolites in rats by HPLC‐IT‐TOF‐MSn.

Author

Wang, Jing‐Zhe, Zhang, Yi‐Fan, Xu, Feng, Shang, Ming‐Ying, Liu, Guang‐Xue, and Cai, Shao‐Qing

Abstract

Abstract: Harpagoside, an iridoid glycoside, is the major bioactive constituent of the traditional Chinese medicine Scrophulariae Radix. High‐performance liquid chromatography with a diode array detector combined with electrospray ionization ion trap time‐of‐flight multistage mass spectrometry (HPLC‐ESI‐IT‐TOF‐MSn) was used to profile and identify the metabolites of harpagoside in rats in vivo and to study the distribution of these metabolites in rats for the first time. A total of 45 metabolites were identified, 37 of which were postulated to be new compounds. The number of detected metabolites in the heart, liver, spleen, lung, kidney, stomach and small intestine was 2, 9, 6, 16, 4, 16 and 6, respectively, which indicated that the target organs of harpagoside should be spleen, lung and stomach. The main types of metabolic reactions of harpagoside in rats are hydrolysis, reduction, sulfuric acid addition, hydroxylation, methoxylation, sulfate substitution, methylation, glucose conjugation and amino acid conjugation. Furthermore, 23 metabolites were determined to have bioactivities based on the literature and ‘PharmMapper’ analysis. These findings are useful for better comprehension of the effective forms, target organs and pharmacological effects of harpagoside. Moreover, these findings provide a reference for studying the metabolism and distribution of iridoid compounds. [ABSTRACT FROM AUTHOR]

Published
2018
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