Search

Your search keyword '"Shan, Tingyu"' showing total 29 results
Start Over Author "Shan, Tingyu"
29 results on '"Shan, Tingyu"'

12. Full‐length transcriptome sequencing provides new insights into the complexity of flavonoid biosynthesis in Glechoma longituba.

Author

Shan, Tingyu, Xu, Jingyao, Zhong, Xinxin, Zhang, Jingjing, He, Bing, Tao, Yijia, and Wu, Jiawen

Subjects
*FLAVONOLS, *FLAVONOIDS, *BIOSYNTHESIS, *LINCRNA, *MICROSATELLITE repeats, *GENE expression, *PEARSON correlation (Statistics)
Abstract

Glechoma longituba has been frequently used in treating urolithiasis and cholelithiasis due to the presence of flavonoids, which are its major bioactive constituents. However, research on the molecular background of flavonoid biosynthesis in G. longituba is limited. In this study, we used single‐molecule real‐time combined with next‐generation sequencing technologies to construct the complete transcriptome of G. longituba. We identified 404,648 non‐redundant transcripts, including 249,697 coding sequences, 197,811 simple sequence repeats, 174,846 long noncoding RNA, and 176,554 coding RNA. Moreover, we functionally annotated 346,218 isoforms (85.56%) and identified 86,528 differentially expressed genes. We also identified 55 non‐redundant full‐length isoforms related to the flavonoid biosynthetic pathway. Pearson correlation analysis revealed that the expression levels of some key genes of the flavonoid biosynthesis pathway were significantly positively correlated with the flavonoid metabolites. Furthermore, we performed bioinformatics analysis (sequence and structural) of isoform_47029 (encoding flavanone 3‐hydroxylase) and isoform_53692 (encoding flavonol synthase) to evaluate their potential biological functions. Finally, we validated gene expression levels of 12 flavonoid‐related key enzyme genes using quantitative real‐time PCR. Overall, this study provides full‐length transcriptome information on G. longituba for the first time and valuable molecular resources for further research on the medicinal properties of this plant. [ABSTRACT FROM AUTHOR]

Published
2023
Full Text
View/download PDF

22. Additional file 1 of Genetic basis of maize kernel oil-related traits revealed by high-density SNP markers in a recombinant inbred line population

Author

Fang, Hui, Fu, Xiuyi, Ge, Hanqiu, Zhang, Aixia, Shan, Tingyu, Wang, Yuandong, Li, Ping, and Wang, Baohua

Abstract

Additional file 1: Figure S1. Recombination bin map of 180 lines in the KUI3/SC55 RIL population. Figure S2. Phenotypic distributions of 19 oil-related traits in the KUI3/SC55 RIL population. Figure S3. Pearson correlation coefficients (upper right) for the oil-related traits and -log10 (P-value) of the Pearson correlation (bottom left). Figure S4. LOD profiles for the QTL clusters that colocalized with previously cloned genes. Figure S5. Functional category annotations for 19 colocalized genes. Figure S6. Heat map of gene expression for 19 colocalized genes in developing embryo, endosperm, and seed at various developing stages.

Published
2021
Full Text
View/download PDF

24. Additional file 1 of Full-length transcriptome sequences by a combination of sequencing platforms applied to isoflavonoid and triterpenoid saponin biosynthesis of Astragalus mongholicus Bunge

Author

Yin, Minzhen, Chu, Shanshan, Shan, Tingyu, Zha, Liangping, and Peng, Huasheng

Abstract

Additional file 1: Figure S1. Sequencing results of Pacbio sequel platform. a Length distribution of subreads. b The number and length distributions of FLNC reads. c The number and length distributions of non-redundant transcript isoforms.

Published
2021
Full Text
View/download PDF

25. Additional file 5 of Full-length transcriptome sequences by a combination of sequencing platforms applied to isoflavonoid and triterpenoid saponin biosynthesis of Astragalus mongholicus Bunge

Author

Yin, Minzhen, Chu, Shanshan, Shan, Tingyu, Zha, Liangping, and Peng, Huasheng

Abstract

Additional file 5: Figure S2. Statistics of transcripts and differentially expressed genes. a Venn diagram of transcripts in different organs. b, c, d Scatterplot of the GO functional enrichment of DEGs (AR vs. AL, AR vs. AS and AL vs. AS, respectively).

Published
2021
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results