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24 results on '"Shaheed, Sadr ul"'

6. Oncoproteomic applications for detection of breast cancer : proteomic profiling of breast cancer models and biopsies

Author

Shaheed, Sadr-ul

Subjects
570, Breast cancer, Detection, Biomarkers, Proteomics, Bio-fluids, Nipple Aspirate Fluid, Luminal, Basal, Biopsies.
Abstract

The heterogeneity of breast cancer (disease stage and phenotype) makes it challenging to differentiate between each subtype; luminal A, luminal B, HER2, basal-like and claudin-low, on the basis of a single gene or protein. Therefore, a collection of markers is required that can serve as a signature for diagnosing different types of breast cancer. New developments in proteomics have provided the opportunity to look at phenotype-specific breast cancer cell lines and stage-specific liquid biopsies (nipple aspirate fluid [NAF], plasma samples) to identify disease and phenotype specific signature. An 8-plex iTRAQ quantification strategy was employed to compare proteomic profiles of a range of breast cancer and ‘normal-like’ cell lines with primary breast epithelial cells. From this, 2467 proteins were identified on Orbitrap Fusion and Ultraflex II, of which 1430 were common. Matched pairs of NAF samples from four patients with different stages of breast cancer, were analysed by SCX-LC-MS and a total of 1990 unique gene products were identified. More than double the number of proteins previously published data, were detected in NAF, including 300 not detected in plasma. The NAF from the diseased patients have 138 potential phenotype biomarkers that were significantly changed compared to the healthy volunteer (7 for luminal A, 9 for luminal B, 11 for HER2, 14 for basal-like and 52 for claudin-low type). The average coefficient of variation for triplicate analyses by multiple reaction monitoring mass spectrometry (MRM-MS), was 9% in cell lines, 17 % in tissue biopsies, 22% in serum samples and 24% in NAF samples. Overall, the results provide a strong paradigm to develop a clinical assay based on proteomic changes in NAF samples for the early detection of breast cancer supplementary to established mammography programmes.

Published
2017

13. Identification of Stage-Specific Breast Markers Using Quantitative Proteomics

Author

Shaheed, Sadr-ul, primary, Rustogi, Nitin, additional, Scally, Andrew, additional, Wilson, Julie, additional, Thygesen, Helene, additional, Loizidou, Maria A., additional, Hadjisavvas, Andreas, additional, Hanby, Andrew, additional, Speirs, Valerie, additional, Loadman, Paul, additional, Linforth, Richard, additional, Kyriacou, Kyriacos, additional, and Sutton, Chris W., additional

Published
2013
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14. Metabolic Carcinogenesis

Author

Ali, Mahwish, Farhat, Syeda Mehpara, Haleem, Abdul, Rosen, Steven T., Series Editor, Saeed, Rida Fatima, editor, and Shaheed, Sadr ul, editor

Published
2024
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15. Dietary Pattern and Cancer

Author

Mumtaz, Sara, Akhtar, Nosheen, Ahmed, Awais, Qazi, Asma Saleem, Rosen, Steven T., Series Editor, Saeed, Rida Fatima, editor, and Shaheed, Sadr ul, editor

Published
2024
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18. Nutritional Genomics and Cancer Prevention

Author

Aslam, Shaista, Iqbal, Riffat, Saeed, Rida Fatima, Akram, Nuzhat, Ijaz, Farhat, Liaqat, Irfana, Aslam, Ahmed Sohaib, Rosen, Steven T., Series Editor, Saeed, Rida Fatima, editor, and Shaheed, Sadr ul, editor

Published
2024
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19. Diet, Gut Microbes, and Cancer

Author

Qazi, Asma Saleem, Rahman, Ubaid ur, Ahmad, Bilal, Safdar, Waseem, Ahmad, Saeed, Mumtaz, Sara, Rosen, Steven T., Series Editor, Saeed, Rida Fatima, editor, and Shaheed, Sadr ul, editor

Published
2024
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20. Introduction to Nutrition and Cancer

Author

Ahmedah, Hanadi Talal, Basheer, Haneen Adel, Almazari, Inas, Amawi, Kawther Faisal, Rosen, Steven T., Series Editor, Saeed, Rida Fatima, editor, and Shaheed, Sadr ul, editor

Published
2024
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24. Nipple aspirate fluid-A liquid biopsy for diagnosing breast health.

Author

Shaheed SU, Tait C, Kyriacou K, Mullarkey J, Burrill W, Patterson LH, Linforth R, Salhab M, and Sutton CW

Subjects
Breast Neoplasms metabolism, Female, Humans, Liquid Biopsy, Breast Neoplasms diagnosis, Breast Neoplasms pathology, Nipple Aspirate Fluid metabolism, Proteomics
Abstract

Purpose: Nipple secretions are protein-rich and a potential source of breast cancer biomarkers for breast cancer screening. Previous studies of specific proteins have shown limited correlation with clinicopathological features. Our aim, in this pilot study, was to investigate the intra- and interpatient protein composition of nipple secretions and the implications for their use as liquid biopsies., Experimental Design: Matched pairs of nipple discharge/nipple aspirate fluid (NAF, n = 15) were characterized for physicochemical properties and SDS-PAGE. Four pairs were selected for semiquantitative proteomic profiling and trypsin-digested peptides analyzed using 2D-LC Orbitrap Fusion MS. The resulting data were subject to bioinformatics analysis and statistical evaluation for functional significance., Results: A total of 1990 unique proteins were identified many of which are established cancer-associated markers. Matched pairs shared the greatest similarity (average Pearson correlation coefficient of 0.94), but significant variations between individuals were observed., Conclusions and Clinical Relevance: This was the most complete proteomic study of nipple discharge/nipple aspirate fluid to date providing a valuable source for biomarker discovery. The high level of milk proteins in healthy volunteer samples compared to the cancer patients was associated with galactorrhoea. Using matched pairs increased confidence in patient-specific protein levels but changes relating to cancer stage require investigation of a larger cohort., (© 2017 The Authors. PROTEOMICS-Clinical Applications published by WILEY-VCH Verlag GmbH & Co. KGaA.)

Published
2017
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