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Copyright of Genetika (0534-0012) is the property of Serbian Genetics Society and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Present study evaluated the performance of Mentha spicata extract as cost effective substitute for synthetic growth regulator (GA3) and vitamins in Murashige and Skoog medium for micropropagation of potato varieties. Based on the results of pilot study 100ml per liter was selected as most suitable concentration to be used for experiment. Data for various traits viz; root initiation, root length, number of roots, bud proliferation, shoot length, number of shoots, number of nodes and inter-nodal length was recorded. Overall growth response of potato plants on the medium supplemented with Mentha spicata extract was parallel and significantly different from the normal medium without any adverse effect on plant health. Plantlets showed better health, root growth, shoot growth and morphology. The cost of using Mentha spicata extract per plant-let was found to be 75% lower than plantlets grown on standard medium. It is strongly recommended that natural growth substance function as growth stimulator and showed best performance. This strongly suggests that 100ml per liter Mentha spicata is suitable and cheaper alternative for very expensive and more frequently used constituent (GA3) and vitamins for micro-propagation. [ABSTRACT FROM AUTHOR]

In the current studies calli of Oryza sativa. L. cv. Swat-1 were incrementally adapted to ionic stress (25 mM LiCl) to asses some of the adaptation mechanism(s). Adapted calli line was obtained on MS medium supplemented with 2,4-D (2 mg/l) and kinetin (0.25 mg/l). For regeneration MS medium with 1.0mg/l NAA and BAP + 30g/l D-Sorbitol was used. LiCl adapted line showed significantly higher indices of tolerance than unadapted line at 100, 150, 200 and 250 mM NaCl stress. Adaptation resulted in enhanced accumulation of saturated fatty acids (myristic and stearic acids), while unsaturated fatty acids exhibited variable profiles with enhanced oleic acid and significantly reduced linoleic acid. The regeneration capability of adapted line decreased 10 times (20% to 2%), while that of unadapted line kept on increasing (26% to 38%) at each subsequent subculture on regeneration medium. Addition of 10 mM LiCl resulted in enhanced and earlier regeneration in adapted line. Complete plantlets were obtained in about ten days while normally plantlets were obtained after 28 days. [ABSTRACT FROM AUTHOR]