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39 results on '"Sha, Michael Y."'

4. A Highly Sensitive XNA-Based RT-qPCR Assay for the Identification of ALK, RET, and ROS1 Fusions in Lung Cancer.

Author

Lee, Bongyong, Chern, Andrew, Fu, Andrew Y., Zhang, Aiguo, and Sha, Michael Y.

Subjects
LUNG cancer, ONCOLOGY, GENE fusion, GENE expression, PROTEIN-tyrosine kinase inhibitors, NUCLEIC acids
Abstract

Lung cancer is often triggered by genetic alterations that result in the expression of oncogenic tyrosine kinases. Specifically, ALK, RET, and ROS1 chimeric receptor tyrosine kinases are observed in approximately 5–7%, 1–2%, and 1–2% of NSCLC patients, respectively. The presence of these fusion genes determines the response to tyrosine kinase inhibitors. Thus, accurate detection of these gene fusions is essential in cancer research and precision oncology. To address this need, we have developed a multiplexed RT-qPCR assay using xeno nucleic acid (XNA) molecular clamping technology to detect lung cancer fusions. This assay can quantitatively detect thirteen ALK, seven ROS1, and seven RET gene fusions in FFPE samples. The sensitivity of the assay was established at a limit of detection of 50 copies of the synthetic template. Our assay has successfully identified all fusion transcripts using 50 ng of RNA from both reference FFPE samples and cell lines. After validation, a total of 77 lung cancer patient FFPE samples were tested, demonstrating the effectiveness of the XNA-based fusion gene assay with clinical samples. Importantly, this assay is adaptable to highly degraded RNA samples with low input amounts. Future steps involve expanding the testing to include a broader range of clinical samples as well as cell-free RNAs to further validate its applicability and reliability. [ABSTRACT FROM AUTHOR]

Published
2024
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8. A new testing platform using fingerstick blood for quantitative antibody response evaluation after SARS-CoV-2 vaccination

Author

Du, Jinwei, primary, Zhang, Dayu, additional, Pathakamuri, Joseph A., additional, Kuebler, Daniel, additional, Yang, Ying, additional, Loginova, Yulia, additional, Chu, Eric, additional, Madej, Roberta, additional, Neves, Jocelyn V., additional, Singer, Brianna, additional, Radke, Holly, additional, Spencer, Naomi, additional, Rizk, Elizabeth, additional, Zhang, Aiguo, additional, Lu, Chuanyi M., additional, and Sha, Michael Y., additional

Published
2022
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26. Abstract 1703: New genetic variations discovered in KRAS wild-type cetuximab resistant Chinese colorectal cancer patients

Author

Jing, Changwen, primary, Wang, Ting, additional, Ma, Rong, additional, Cao, Haixia, additional, Xu, EnShun, additional, Wang, Zhuo, additional, Liu, Siwen, additional, Chen, Dan, additional, Zhang, Junying, additional, Wu, Yang, additional, Zhang, Yuan, additional, Ding, Ping, additional, Sha, Michael Y., additional, Wu, Jianzhong, additional, and Feng, Jifeng, additional

Published
2019
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29. Surface-enhanced Raman scattering tags for rapid and homogeneous detection of circulating tumor cells in the presence of human whole blood

Author

Sha, Michael Y., Hongxia Xu, Natan, Michael J., and Cromer, Remy

Subjects
Biological markers -- Usage, Cancer cells -- Evaluation, Raman effect -- Usage, Chemistry
Abstract

A novel application of Nanoplex biotags is described for the direct detection of rare cancer cells in whole blood. Multiplexed labeling of biomarkers with multiplex surface-enhanced Raman scattering (SERS) tags has improved the accuracy and specificity of assay.

Published
2008

30. Coupling molecular beacons to barcoded metal nanowires for multiplexed, sealed chamber DNA bioassays

Author

Stoermer, Rebecca L., Cederquist, Kristin B., McFarland, Sean K., Sha, Michael Y., Penn, Sharron G., and Keating, Christine D.

Subjects
Biological assay -- Research, Nucleic acids -- Chemical properties, Chemistry
Abstract

Various probe designs and experimental parameters for no-wash, sealed chamber and multiplexed detection of nucleic acids by combining molecular beacon (MB) probes, as well as the barcoded metal nanowires are discussed. The results show that such nanowires based MB assays are extremely helpful, as they help in reducing assay complexity and the risk of contamination to a very large extent and hence require no further sample manipulations.

Published
2006

31. Use of Nanobarcodes® Particles in Bioassays

Author

Freeman, R. Griffith, primary, Raju, Paul A., additional, Norton, Scott M., additional, Walton, Ian D., additional, Smith, Patrick C., additional, He, Lin, additional, Natan, Michael J., additional, Sha, Michael Y., additional, and Penn, Sharron G., additional

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37. Use of Nanobarcodes® Particles in Bioassays.

Author

Walker, John M., Rosenthal, Sandra J., Wright, David W., Freeman, R. Griffith, Raju, Paul A., Norton, Scott M., Walton, Ian D., Smith, Patrick C., He, Lin, Natan, Michael J., Sha, Michael Y., and Penn, Sharron G.

Abstract

We have developed striped metal nanoparticles, Nanobarcodes® particles, which can act as encoded substrates in multiplexed assays. These particles are metallic, encodeable, machine-readable, durable, submicron-sized tags. The power of this technology is that the particles are intrinsically encoded by virtue of the difference in reflectivity of adjacent metal stripes. This chapter describes protocols for the attachment of biological molecules, and the subsequent use of the Nanobarcodes particles in bioassays. [ABSTRACT FROM AUTHOR]

Published
2005
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39. Use of nanobarcodes particles in bioassays.

Author

Freeman RG, Raju PA, Norton SM, Walton ID, Smith PC, He L, Natan MJ, Sha MY, and Penn SG

Subjects
Animals, Humans, Gene Expression Profiling methods, Nanostructures chemistry, Nanotechnology methods, Oligonucleotides chemistry
Abstract

We have developed striped metal nanoparticles, Nanobarcodes particles, which can act as encoded substrates in multiplexed assays. These particles are metallic, encodeable, machine-readable, durable, submicron-sized tags. The power of this technology is that the particles are intrinsically encoded by virtue of the difference in reflectivity of adjacent metal stripes. This chapter describes protocols for the attachment of biological molecules, and the subsequent use of the Nanobarcodes particles in bioassays.

Published
2005
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