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Patient experience of nursing care is the perception of the patient about the real existing nursing service. Addressing patient experience of nursing care is very important to improve nursing service quality because it identifies the factors that affect the nursing care quality better than patient satisfaction. Therefore, this study aimed to assess patient experience in nursing care and associated factors among adult admitted patients in Debre Markos and Dessie referral hospitals. An institution-based cross-sectional study was conducted from March 1, 2019, to March 30, 2019, among 528 consecutively selected adult admitted patients. Data were cleaned, coded, and entered in Epi-data version 3.1 then exported to Statistical Package for Social Sciences version 25 for analysis. Multivariate logistic regression, with a 95% CI was used to identify variables that had a significant association. The overall good patient experience in nursing care was 64%. Duration of admission ≥22 days (adjusted odds ratio [AOR] = 2.67, 95% CI = 1.013-7.025) and free service (AOR = 3.69, 95% CI = 2.381-5.730) showed a positive association with patient experience in nursing care. However, admission in gynecology ward (AOR = 0.43, 95%CI = 0.257-0.707), secondary education (AOR = 0.53, 95% CI: 0.308-0.907), and college or above education (AOR = 0.55, 95%CI = 0.320-0.957) showed a negative association with patient experience in nursing care.