Search

Staphylococcus aureus infections present a significant threat to the global healthcare system. The increasing resistance to existing antibiotics and their limited efficacy underscores the urgent need to identify new antibacterial agents with low toxicity to effectively combat various S. aureus infections. Hence, in this study, we have screened T-muurolol for possible interactions with several S. aureus-specific bacterial proteins to establish its potential as an alternative antibacterial agent. Based on its binding affinity and interactions with amino acids, T-muurolol was identified as a potential inhibitor of S. aureus lipase, dihydrofolate reductase, penicillin-binding protein 2a, D-Ala:D-Ala ligase, and ribosome protection proteins tetracycline resistance determinant (RPP TetM), which indicates its potentiality against S. aureus and its multi-drug-resistant strains. Also, T-muurolol exhibited good antioxidant and anti-inflammatory activity by showing strong binding interactions with flavin adenine dinucleotide (FAD)-dependent nicotinamide adenine dinucleotide phosphate (NAD(P)H) oxidase, and cyclooxygenase-2. Consequently, molecular dynamics (MD) simulation and recalculating binding free energies elucidated its binding interaction stability with targeted proteins. Furthermore, quantum chemical structure analysis based on density functional theory (DFT) depicted a higher energy gap between the highest occupied molecular orbital and lowest unoccupied molecular orbital (EHOMO-LUMO) with a lower chemical potential index, and moderate electrophilicity suggests its chemical hardness and stability and less polarizability and reactivity. Additionally, pharmacological parameters based on ADMET, Lipinski's rules, and bioactivity score validated it as a promising drug candidate with high activity toward ion channel modulators, nuclear receptor ligands, and enzyme inhibitors. In conclusion, the current findings suggest T-muurolol as a promising alternative antibacterial agent that might be a potential phytochemical-based drug against S. aureus. This study also suggests further clinical research before human application. [ABSTRACT FROM AUTHOR]

Melissa officinalis L., a well-known herb with diverse industrial and ethnopharmacological properties. Although, there has been a significant lack in the breeding attempts of this invaluable herb. This study aimed to enhance the agronomical traits of M. officinalis through in vitro polyploidization. Nodal segments were micropropagated and subjected to oryzalin treatment at concentrations of 20, 40, and 60 mM for 24 and 48 hours. Flow cytometry, chromosome counting, and stomatal characteristics were employed to confirm the ploidy level of the surviving plants. The survival rate of the treated explants decreased exponentially with increasing oryzalin concentration and duration. The highest polyploid induction rate (8%) was achieved with 40 mM oryzalin treatment for 24 hours. The induced tetraploid plants exhibited vigorous growth, characterized by longer shoots, larger leaves, and a higher leaf count. Chlorophyll content and fluorescence parameters elucidated disparities in photosynthetic performance between diploid and tetraploid genotypes. Tetraploid plants demonstrated a 75% increase in average essential oil yield, attributed to the significantly larger size of peltate trichomes. Analysis of essential oil composition in diploid and tetraploid plants indicated the presence of three major components: geranial, neral, and citronellal. While citronellal remained consistent, geranial and neral increased by 11.06% and 9.49%, respectively, in the tetraploid population. This effective methodology, utilizing oryzalin as an anti-mitotic agent for polyploid induction in M. officinalis, resulted in a polyploid genotype with superior morpho-physiological traits. The polyploid lemon balm generated through this method has the potential to meet commercial demands and contribute significantly to the improvement of lemon balm cultivation. [ABSTRACT FROM AUTHOR]

The goal of this article is to predict the further development of the market price of farmland in Czechia. As part of this goal, the environmental and socio-economic factors affecting the quantity and quality of farmland and, subsequently, the amount of its market price will be discussed. In addition to the quality of the farmland, the market price of individual plots of land is influenced by a number of other factors, such as the size and shape of the plot, location, erosion risk, the concluded usufructuary lease agreements, competition from buyers in the vicinity, and other factors. The average market price of farmland in Czechia was 29.4 CZK/m2 in 2021, which represents an increase of 16.1% against the amount of 25.3 CZK/m2 in 2020. According to the results of the statistical analysis, it is possible to assume a constant increase in the prices of farmland precisely because of the ever-decreasing area of land intended for agricultural production. Another factor for the increase in prices can be increased inflation. In 2027, market prices for farmland could reach up to 40 CZK per m2. Nevertheless, Czechia is among the countries with the lowest market price of farmland; the price is even lower than in neighboring Poland or Spain. This also contributes to the fact that the market prices of farmland in Czechia are increasing at a relatively fast pace every year. [ABSTRACT FROM AUTHOR]

This paper explores the long-term persistence of interregional differences in self-employment rates. The analysis, based on spatial Durbin error models, was performed for 206 Czech regions from 1930 to 2011. The results obtained supported the 'persistence claim' because regional self-employment rates in 1930 were found to be positively and significantly associated with those obtained in 1991, 2001 and 2011. Moreover, this study contributes to the knowledge about transmission channels of historical entrepreneurial experience. First, a new mechanism related to the career and educational paths of individuals with a pre-socialist entrepreneurial family background is suggested. Second, the newcomers' capacity to absorb knowledge and skills is emphasized as crucial for the functioning of pre-socialist 'entrepreneurial memory'. [ABSTRACT FROM AUTHOR]

Copyright of Waste Forum is the property of Czech Environment Management Center (CEMC) and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Listy Cukrovarnicke a Reparske is the property of VUC Praha a.s. and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Listy Cukrovarnicke a Reparske is the property of VUC Praha a.s. and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Listy Cukrovarnicke a Reparske is the property of VUC Praha a.s. and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Listy Cukrovarnicke a Reparske is the property of VUC Praha a.s. and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Listy Cukrovarnicke a Reparske is the property of VUC Praha a.s. and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Waste Forum is the property of Czech Environment Management Center (CEMC) and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Fundraising can be viewed as a systematic activity aimed primarily at raising financial resources for the activities of either an organization or an individual. The inconsistency of this effort usually cannot be blamed on internal factors within an organization, but rather phenomena of a macroeconomic, legislative or societal nature. In an effort to gain an insight into the measure of utilizing fundraising principles in nonprofit organizations, extensive research was conducted for the ninth time among nonprofit organizations in the Czech Republic, the sample of which consisted of 313 fully completed questionnaires. Based on the research results focusing on fundraising, measures were proposed to expand the possibilities of ensuring effective multisource funding for a selected nonprofit organization. One of these options and at the same time the current trend in marketing not only for the nonprofit sector is crowdfunding. Crowdfunding is a new concept of funding projects that has seen a significantly growing trend in the fundraising is part of marketing activities, or at least it is very closely connected with them. [ABSTRACT FROM AUTHOR]