1. Innate Immune Landscape in Early Lung Adenocarcinoma by Paired Single-Cell Analyses
- Author
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Catherine Sanders, Mary Beth Beasley, El-ad David Amir, Benjamin Greenbaum, Hanjie Li, Andrew Leader, Andrea S. Wolf, Klaus Meinhof, Adeeb Rahman, Marissa Vignali, Raja M. Flores, Chiara Medaglia, Yonit Lavin, Romain Remark, Naama Elefant, Dana Pe'er, Camille Bigenwald, Soma Kobayashi, Ido Amit, Ryan O. Emerson, Seunghee Kim-Shulze, Jacob H. Levine, Christian Becker, Julie A. Rytlewski, Miriam Merad, Alexander Solovyov, Andrew Chow, Sacha Gnjatic, and Robert Sweeney
- Subjects
0301 basic medicine ,Myeloid ,Innate immune system ,T cell ,medicine.medical_treatment ,Cell ,Immunotherapy ,biochemical phenomena, metabolism, and nutrition ,Biology ,medicine.disease ,General Biochemistry, Genetics and Molecular Biology ,03 medical and health sciences ,030104 developmental biology ,medicine.anatomical_structure ,Immune system ,Immunity ,Immunology ,medicine ,bacteria ,Adenocarcinoma - Abstract
Summary To guide the design of immunotherapy strategies for patients with early stage lung tumors, we developed a multiscale immune profiling strategy to map the immune landscape of early lung adenocarcinoma lesions to search for tumor-driven immune changes. Utilizing a barcoding method that allows a simultaneous single-cell analysis of the tumor, non-involved lung, and blood cells, we provide a detailed immune cell atlas of early lung tumors. We show that stage I lung adenocarcinoma lesions already harbor significantly altered T cell and NK cell compartments. Moreover, we identified changes in tumor-infiltrating myeloid cell (TIM) subsets that likely compromise anti-tumor T cell immunity. Paired single-cell analyses thus offer valuable knowledge of tumor-driven immune changes, providing a powerful tool for the rational design of immune therapies. Video Abstract
- Published
- 2017
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