1. The Third SeaWiFS HPLC Analysis Round-Robin Experiment (SeaHARRE-3)
- Author
-
Hooker, Stanford B, VanHeukelem, Laurei, Thomas, Crystal S, Claustre, Herve, Ras, Josephine, Schluter, Louise, Clementson, Lesley, vanderLinde, Dirk, Eker-Develi, Elif, Berthon, Jean-Francois, Barlow, Ray, Sessions, Heather, Ismail, Hassan, and Perl, Jason
- Subjects
Inorganic, Organic And Physical Chemistry - Abstract
Seven international laboratories specializing in the determination of marine pigment concentrations using high performance liquid chromatography (HPLC) were intercompared using in situ samples and a mixed pigment sample. The field samples were collected primarily from oligotrophic waters, although mesotrophic and eutrophic waters were also sampled to create a dynamic range in chlorophyll concentration spanning approximately two orders of magnitude (0.020 1.366 mg m^{-3}) The intercomparisons were used to establish the following: a) the uncertainties in quantitating individual pigments and higher-order variables (sums, ratios, and indices); b) the reduction in uncertainties as a result of applying quality assurance (QA) procedures; c) the importance of establishing a properly defined referencing system in the computation of uncertainties; d) the analytical benefits of performance metrics, and e) the utility of a laboratory mix in understanding method performance. In addition, the remote sensing requirements for the in situ determination of total chlorophyll a were investigated to determine whether or not the average uncertainty for this measurement is being satisfied.
- Published
- 2009