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27,508 results on '"Service de chirurgie"'

1. Decellularized vascularized bone grafts: A preliminary in vitro porcine model for bioengineered transplantable bone shafts

Author: UCL - SSS/IREC/CHEX - Pôle de chirgurgie expérimentale et transplantation, UCL - SSS/IREC/MORF - Pôle de Morphologie, UCL - SSS/IREC/NMSK - Neuro-musculo-skeletal Lab, UCL - (SLuc) Service d'orthopédie et de traumatologie de l'appareil locomoteur, UCL - (SLuc) Service de stomatologie et de chirurgie maxillo-faciale, UCL - (SLuc) Service de chirurgie et transplantation abdominale, UCL - (SLuc) Service de chirurgie plastique, Rougier, Guillaume, Maistriaux, Louis, Fievé, Lies, Xhema, Daela, Evrard, Robin, Manon, Julie, Olszewski, Raphaël, Szmytka, Fabien, Thurieau, Nicolas, Boisson, Jean, Kadlub, Natacha, Gianello, Pierre, Behets Wydemans, Catherine, Lengelé, Benoît, UCL - SSS/IREC/CHEX - Pôle de chirgurgie expérimentale et transplantation, UCL - SSS/IREC/MORF - Pôle de Morphologie, UCL - SSS/IREC/NMSK - Neuro-musculo-skeletal Lab, UCL - (SLuc) Service d'orthopédie et de traumatologie de l'appareil locomoteur, UCL - (SLuc) Service de stomatologie et de chirurgie maxillo-faciale, UCL - (SLuc) Service de chirurgie et transplantation abdominale, UCL - (SLuc) Service de chirurgie plastique, Rougier, Guillaume, Maistriaux, Louis, Fievé, Lies, Xhema, Daela, Evrard, Robin, Manon, Julie, Olszewski, Raphaël, Szmytka, Fabien, Thurieau, Nicolas, Boisson, Jean, Kadlub, Natacha, Gianello, Pierre, Behets Wydemans, Catherine, and Lengelé, Benoît
Abstract: INTRODUCTION: Durable reconstruction of critical size bone defects is still a surgical challenge despite the availability of numerous autologous and substitute bone options. In this paper, we have investigated the possibility of creating a living bone allograft, using the perfusion/decellularization/recellularization (PDR) technique, which was applied to an original model of vascularized porcine bone graft. MATERIALS AND METHODS: 11 porcine bone forelimbs, including radius and ulna, were harvested along with their vasculature including the interosseous artery and then decellularized using a sequential detergent perfusion protocol. Cellular clearance, vasculature, extracellular matrix (ECM), and preservation of biomechanical properties were evaluated. The cytocompatibility and in vitro osteoinductive potential of acellular extracellular matrix were studied by static seeding of NIH-3T3 cells and porcine adipose mesenchymal stem cells (pAMSC), respectively. RESULTS: The vascularized bone grafts were successfully decellularized, with an excellent preservation of the 3D morphology and ECM microarchitecture. Measurements of DNA and ECM components revealed complete cellular clearance and preservation of ECM’s major proteins. Bone mineral density (BMD) acquisitions revealed a slight, yet non-significant, decrease after decellularization, while biomechanical testing was unmodified. Cone beam computed tomography (CBCT) acquisitions after vascular injection of barium sulphate confirmed the preservation of the vascular network throughout the whole graft. The non-toxicity of the scaffold was proven by the very low amount of residual sodium dodecyl sulfate (SDS) in the ECM and confirmed by the high live/dead ratio of fibroblasts seeded on periosteum and bone ECM-grafts after 3, 7, and 16 days of culture. Moreover, cell proliferation tests showed a significant multiplication of seeded cell populations at the same endpoints. Lastly, the differentiation study using pAMSC confirmed t
Published: 2023

2. A New Osteogenic Membrane to Enhance Bone Healing: At the Crossroads between the Periosteum, the Induced Membrane, and the Diamond Concept

Author: UCL - SSS/IREC/CHEX - Pôle de chirgurgie expérimentale et transplantation, UCL - SSS/IREC/MORF - Pôle de Morphologie, UCL - SSS/IREC/NMSK - Neuro-musculo-skeletal Lab, UCL - SST/IMCN/BSMA - Bio and soft matter, UCL - (SLuc) Service d'orthopédie et de traumatologie de l'appareil locomoteur, UCL - (SLuc) Service de chirurgie et transplantation abdominale, UCL - (SLuc) Service de chirurgie plastique, Manon, Julie, Evrard, Robin, Fievé, Lies, Bouzin, Caroline, Magnin, Delphine, Xhema, Daela, Darius, Tom, Bonaccorsi Riani, Eliano, Gianello, Pierre, Docquier, Pierre-Louis, Schubert, Thomas, Lengelé, Benoît, Behets Wydemans, Catherine, Cornu, Olivier, UCL - SSS/IREC/CHEX - Pôle de chirgurgie expérimentale et transplantation, UCL - SSS/IREC/MORF - Pôle de Morphologie, UCL - SSS/IREC/NMSK - Neuro-musculo-skeletal Lab, UCL - SST/IMCN/BSMA - Bio and soft matter, UCL - (SLuc) Service d'orthopédie et de traumatologie de l'appareil locomoteur, UCL - (SLuc) Service de chirurgie et transplantation abdominale, UCL - (SLuc) Service de chirurgie plastique, Manon, Julie, Evrard, Robin, Fievé, Lies, Bouzin, Caroline, Magnin, Delphine, Xhema, Daela, Darius, Tom, Bonaccorsi Riani, Eliano, Gianello, Pierre, Docquier, Pierre-Louis, Schubert, Thomas, Lengelé, Benoît, Behets Wydemans, Catherine, and Cornu, Olivier
Abstract: The lack of viability of massive bone allografts for critical-size bone defect treatment remains a challenge in orthopedic surgery. The literature has reviewed the advantages of a multi-combined treatment with the synergy of an osteoconductive extracellular matrix (ECM), osteogenic stem cells, and growth factors (GFs). Questions are still open about the need for ECM components, the influence of the decellularization process on the latter, the related potential loss of function, and the necessity of using pre-differentiated cells. In order to fill in this gap, a bone allograft surrounded by an osteogenic membrane made of a decellularized collagen matrix from human fascia lata and seeded with periosteal mesenchymal stem cells (PMSCs) was analyzed in terms of de-/recellularization, osteogenic properties, PMSC self-differentiation, and angiogenic potential. While the decellularization processes altered the ECM content differently, the main GF content was decreased in soft tissues but relatively increased in hard bone tissues. The spontaneous osteogenic differentiation was necessarily obtained through contact with a mineralized bone matrix. Trying to deepen the knowledge on the complex matrix–cell interplay could further propel these tissue engineering concepts and lead us to provide the biological elements that allow bone integration in vivo.
Published: 2023

3. Exceptional manifestation of Madelung's disease after liver transplantation.

Author: UCL - SSS/IREC - Institut de recherche expérimentale et clinique, UCL - (SLuc) Service de chirurgie plastique, UCL - SSS/IREC/MORF - Pôle de Morphologie, UCL - (SLuc) Service de chirurgie et transplantation abdominale, UCL - SSS/IREC/CHEX - Pôle de chirgurgie expérimentale et transplantation, UCL - (SLuc) Centre de génétique médicale UCL, UCL - (SLuc) Service de gastro-entérologie, Lentini, Audrey, Ciccarelli, Olga, Bonaccorsi Riani, Eliano, Coubeau, Laurent, Revencu, Nicole, Dahlqvist, Géraldine, Lengelé, Benoît, UCL - SSS/IREC - Institut de recherche expérimentale et clinique, UCL - (SLuc) Service de chirurgie plastique, UCL - SSS/IREC/MORF - Pôle de Morphologie, UCL - (SLuc) Service de chirurgie et transplantation abdominale, UCL - SSS/IREC/CHEX - Pôle de chirgurgie expérimentale et transplantation, UCL - (SLuc) Centre de génétique médicale UCL, UCL - (SLuc) Service de gastro-entérologie, Lentini, Audrey, Ciccarelli, Olga, Bonaccorsi Riani, Eliano, Coubeau, Laurent, Revencu, Nicole, Dahlqvist, Géraldine, and Lengelé, Benoît
Abstract: Unlike simple obesity, Madelung's disease (MD) is a rare disease characterized by symmetric accumulation of massive adipose tissue on the neck, the superior part of the trunk and limbs, leading to a pathognomonic cosmetic deformity. Here, we report an extremely rare case of MD associated with bilateral gynecomastia in a 61-year-old man, with a history of recent liver transplantation for alcoholic liver disease (ALD).
Published: 2022

4. L’ostéoinduction des PMSC par différentes matrices issues du système musculosquelettique : Etude In vitro

Author: UCL - SSS/IREC/CHEX - Pôle de chirgurgie expérimentale et transplantation, UCL - SSS/IREC/MORF - Pôle de Morphologie, UCL - SSS/IREC/NMSK - Neuro-musculo-skeletal Lab, UCL - (SLuc) Service d'orthopédie et de traumatologie de l'appareil locomoteur, UCL - (SLuc) Service de chirurgie et transplantation abdominale, UCL - (SLuc) Service de chirurgie plastique, Manon, Julie, Evrard, Robin, Maistriaux, Louis, Fievé, Lies, de Ville de Goyet, Christine, Vanham, P, Schubert, Thomas, Gianello, Pierre, Lengelé, Benoît, Behets Wydemans, Catherine, Cornu, Olivier, UCL - SSS/IREC/CHEX - Pôle de chirgurgie expérimentale et transplantation, UCL - SSS/IREC/MORF - Pôle de Morphologie, UCL - SSS/IREC/NMSK - Neuro-musculo-skeletal Lab, UCL - (SLuc) Service d'orthopédie et de traumatologie de l'appareil locomoteur, UCL - (SLuc) Service de chirurgie et transplantation abdominale, UCL - (SLuc) Service de chirurgie plastique, Manon, Julie, Evrard, Robin, Maistriaux, Louis, Fievé, Lies, de Ville de Goyet, Christine, Vanham, P, Schubert, Thomas, Gianello, Pierre, Lengelé, Benoît, Behets Wydemans, Catherine, and Cornu, Olivier
Published: 2022

5. PMSC differentiation induced by musculoskeletal matrix : in vitro study

Author: UCL - SSS/IREC/CHEX - Pôle de chirgurgie expérimentale et transplantation, UCL - SSS/IREC/MORF - Pôle de Morphologie, UCL - SSS/IREC/NMSK - Neuro-musculo-skeletal Lab, UCL - (SLuc) Service d'orthopédie et de traumatologie de l'appareil locomoteur, UCL - (SLuc) Service de chirurgie et transplantation abdominale, UCL - (SLuc) Service de chirurgie plastique, Manon, Julie, Evrard, Robin, Maistriaux, Louis, Fievé, Lies, de Ville de Goyet, Christine, Vanham, P, Schubert, Thomas, Gianello, Pierre, Lengelé, Benoît, Behets Wydemans, Catherine, Cornu, Olivier, UCL - SSS/IREC/CHEX - Pôle de chirgurgie expérimentale et transplantation, UCL - SSS/IREC/MORF - Pôle de Morphologie, UCL - SSS/IREC/NMSK - Neuro-musculo-skeletal Lab, UCL - (SLuc) Service d'orthopédie et de traumatologie de l'appareil locomoteur, UCL - (SLuc) Service de chirurgie et transplantation abdominale, UCL - (SLuc) Service de chirurgie plastique, Manon, Julie, Evrard, Robin, Maistriaux, Louis, Fievé, Lies, de Ville de Goyet, Christine, Vanham, P, Schubert, Thomas, Gianello, Pierre, Lengelé, Benoît, Behets Wydemans, Catherine, and Cornu, Olivier
Published: 2022

6. Improved massive bone allograft decellularization by perfusion

Author: UCL - SSS/IREC/CHEX - Pôle de chirgurgie expérimentale et transplantation, UCL - SSS/IREC/MORF - Pôle de Morphologie, UCL - SSS/IREC/NMSK - Neuro-musculo-skeletal Lab, UCL - (SLuc) Service d'orthopédie et de traumatologie de l'appareil locomoteur, UCL - (SLuc) Service de chirurgie et transplantation abdominale, UCL - (SLuc) Service de chirurgie plastique, Evrard, Robin, Maistriaux, Louis, Rafferty, Chiara, Manon, Julie, Gianello, Pierre, Cornu, Olivier, Lengelé, Benoît, Schubert, Thomas, UCL - SSS/IREC/CHEX - Pôle de chirgurgie expérimentale et transplantation, UCL - SSS/IREC/MORF - Pôle de Morphologie, UCL - SSS/IREC/NMSK - Neuro-musculo-skeletal Lab, UCL - (SLuc) Service d'orthopédie et de traumatologie de l'appareil locomoteur, UCL - (SLuc) Service de chirurgie et transplantation abdominale, UCL - (SLuc) Service de chirurgie plastique, Evrard, Robin, Maistriaux, Louis, Rafferty, Chiara, Manon, Julie, Gianello, Pierre, Cornu, Olivier, Lengelé, Benoît, and Schubert, Thomas
Published: 2022

7. In vivo massive bone allografts decellularized by a new perfusion process: preliminary results

Author: UCL - SSS/IREC/CHEX - Pôle de chirgurgie expérimentale et transplantation, UCL - SSS/IREC/MORF - Pôle de Morphologie, UCL - SSS/IREC/NMSK - Neuro-musculo-skeletal Lab, UCL - (SLuc) Service d'orthopédie et de traumatologie de l'appareil locomoteur, UCL - (SLuc) Service de chirurgie et transplantation abdominale, UCL - (SLuc) Service de chirurgie plastique, Evrard, Robin, Manon, Julie, Maistriaux, Louis, Darius, Tom, Gianello, Pierre, Lengelé, Benoît, Schubert, Thomas, UCL - SSS/IREC/CHEX - Pôle de chirgurgie expérimentale et transplantation, UCL - SSS/IREC/MORF - Pôle de Morphologie, UCL - SSS/IREC/NMSK - Neuro-musculo-skeletal Lab, UCL - (SLuc) Service d'orthopédie et de traumatologie de l'appareil locomoteur, UCL - (SLuc) Service de chirurgie et transplantation abdominale, UCL - (SLuc) Service de chirurgie plastique, Evrard, Robin, Manon, Julie, Maistriaux, Louis, Darius, Tom, Gianello, Pierre, Lengelé, Benoît, and Schubert, Thomas
Published: 2022

8. Massive bone allograft by a perfusion protocol set up for a biocompatible model

Author: UCL - SSS/IREC/CHEX - Pôle de chirgurgie expérimentale et transplantation, UCL - SSS/IREC/MORF - Pôle de Morphologie, UCL - SSS/IREC/NMSK - Neuro-musculo-skeletal Lab, UCL - (SLuc) Service d'orthopédie et de traumatologie de l'appareil locomoteur, UCL - (SLuc) Service de chirurgie et transplantation abdominale, UCL - (SLuc) Service de chirurgie plastique, Evrard, Robin, Maistriaux, Louis, Rafferty, Chiara, Manon, Julie, Cornu, Olivier, Lengelé, Benoît, Schubert, Thomas, UCL - SSS/IREC/CHEX - Pôle de chirgurgie expérimentale et transplantation, UCL - SSS/IREC/MORF - Pôle de Morphologie, UCL - SSS/IREC/NMSK - Neuro-musculo-skeletal Lab, UCL - (SLuc) Service d'orthopédie et de traumatologie de l'appareil locomoteur, UCL - (SLuc) Service de chirurgie et transplantation abdominale, UCL - (SLuc) Service de chirurgie plastique, Evrard, Robin, Maistriaux, Louis, Rafferty, Chiara, Manon, Julie, Cornu, Olivier, Lengelé, Benoît, and Schubert, Thomas
Published: 2022

9. Malformations vasculaires : un nouvel espoir grâce aux thérapies ciblées antitumorales

Author: UCL - (SLuc) Unité d'oncologie médicale, UCL - (SLuc) Centre du cancer, UCL - SSS/IREC/SLUC - Pôle St.-Luc, UCL - (SLuc) Service de chirurgie plastique, UCL - (SLuc) Service de chirurgie et transplantation abdominale, UCL - SSS/DDUV/GEHU - Génétique, UCL - (SLuc) Centre de malformations vasculaires congénitales, Seront, Emmanuel, Dekeuleneer, Valérie, Coulie, Julien, Van Damme, Ann, Boon, Laurence M., Vikkula, Miikka, UCL - (SLuc) Unité d'oncologie médicale, UCL - (SLuc) Centre du cancer, UCL - SSS/IREC/SLUC - Pôle St.-Luc, UCL - (SLuc) Service de chirurgie plastique, UCL - (SLuc) Service de chirurgie et transplantation abdominale, UCL - SSS/DDUV/GEHU - Génétique, UCL - (SLuc) Centre de malformations vasculaires congénitales, Seront, Emmanuel, Dekeuleneer, Valérie, Coulie, Julien, Van Damme, Ann, Boon, Laurence M., and Vikkula, Miikka
Published: 2022

10. Serial fibrin monomer and D-dimer plasma levels measurements can capture thrombotic complications in critically ill COVID-19 patients: A prospective observational study.

Author: UCL - SSS/IREC/MONT - Pôle Mont Godinne, UCL - (MGD) Service d'anesthésiologie, UCL - (MGD) Services des soins intensifs, UCL - (MGD) Service de chirurgie cardio-vasculaire et thoracique, UCL - (MGD) Laboratoire de biologie clinique, Hardy, Michaël, Michaux, Isabelle, Bulpa, Pierre, Schonau, Benjamin, Nicolay, Benoit, de Maistre, E, Godon, A, Lecompte, T, Mullier, François, UCL - SSS/IREC/MONT - Pôle Mont Godinne, UCL - (MGD) Service d'anesthésiologie, UCL - (MGD) Services des soins intensifs, UCL - (MGD) Service de chirurgie cardio-vasculaire et thoracique, UCL - (MGD) Laboratoire de biologie clinique, Hardy, Michaël, Michaux, Isabelle, Bulpa, Pierre, Schonau, Benjamin, Nicolay, Benoit, de Maistre, E, Godon, A, Lecompte, T, and Mullier, François
Published: 2023

11. Resection of a Giant Middel Mediastinum Paraganglioma by Clamshell Incision without Cardiopulmonary Bypass

Author: UCL - SSS/IREC/MONT - Pôle Mont Godinne, UCL - (MGD) Service d'anatomie pathologique, UCL - (MGD) Service de chirurgie cardio-vasculaire et thoracique, Piette, Wivine, Belhaj, Asmae, Stanciu pop, Claudia, Rondelet, Benoît, UCL - SSS/IREC/MONT - Pôle Mont Godinne, UCL - (MGD) Service d'anatomie pathologique, UCL - (MGD) Service de chirurgie cardio-vasculaire et thoracique, Piette, Wivine, Belhaj, Asmae, Stanciu pop, Claudia, and Rondelet, Benoît
Abstract: Paraganglioma is a tumor developed from paraganglia, which are groups of neuroendocrine cells located along the vascular and nerve axes of the head and neck and along the spine. These tumors are benign and still localized in more than 80% of cases. Middle mediastinal paragangliomas represent less than 1% of mediastinal tumors and grow from the superior or middle mediastinal autonomic ganglion; they mostly are non-functional and are found in older patients. The often large volume and complex location of these tumors make their surgical resection challenging forcing the surgical team to resort to cardiopulmonary bypass and practice transfection of the aortic artery. We successfully operated an 80-year-old patient by clamshell incision without bypass or arterial procedure.
Published: 2023

12. Organ reconditioning and machine perfusion in transplantation

Author: UCL - SSS/IREC/CHEX - Pôle de chirgurgie expérimentale et transplantation, UCL - (SLuc) Service de chirurgie et transplantation abdominale, Bellini, Maria Irene, Bonaccorsi Riani, Eliano, Giorgakis, Emmanouil, Kaisar, Maria E., Patrono, Damiano, Weissenbacher, Annemarie, UCL - SSS/IREC/CHEX - Pôle de chirgurgie expérimentale et transplantation, UCL - (SLuc) Service de chirurgie et transplantation abdominale, Bellini, Maria Irene, Bonaccorsi Riani, Eliano, Giorgakis, Emmanouil, Kaisar, Maria E., Patrono, Damiano, and Weissenbacher, Annemarie
Abstract: Society’s self-sufficiency in terms of organ replacement is still far away from being achieved, given the large disparity between transplant demand and donor organ availability. In the attempt to reduce this discrepancy and expand the donor pool, the transplant community has progressively increased the utilisation of extended-criteria donors (ECD) such as older donors, donors with comorbidities and donation after circulatory death (DCD). The main challenge preventing a wider utilisation of ECD and DCD allografts is the higher susceptibility to the ischemia-reperfusion injury (IRI) (1), an unavoidable part of the transplantation process. For this reason, in the last decades, there has been an exponential development on organ reconditioning strategies, in order to enable graft resuscitation and viability assessment prior to implantation (2).
Published: 2023

13. Decellularized vascularized bone grafts as therapeutic solution for bone reconstruction: A mechanical evaluation.

Author: UCL - SSS/IREC/CHEX - Pôle de chirgurgie expérimentale et transplantation, UCL - SSS/IREC/NMSK - Neuro-musculo-skeletal Lab, UCL - (SLuc) Service d'orthopédie et de traumatologie de l'appareil locomoteur, UCL - (SLuc) Service de chirurgie plastique, Heller, Ugo, Evrard, Robin, Lengelé, Benoît, Schubert, Thomas, Kadlub, Natacha, Boisson, Jean, UCL - SSS/IREC/CHEX - Pôle de chirgurgie expérimentale et transplantation, UCL - SSS/IREC/NMSK - Neuro-musculo-skeletal Lab, UCL - (SLuc) Service d'orthopédie et de traumatologie de l'appareil locomoteur, UCL - (SLuc) Service de chirurgie plastique, Heller, Ugo, Evrard, Robin, Lengelé, Benoît, Schubert, Thomas, Kadlub, Natacha, and Boisson, Jean
Abstract: INTRODUCTION: Large bone defects are challenging for surgeons. Available reimplanted bone substitutes can't properly restore optimal function along and long term osteointegration of the bone graft. Bone substitute based on the perfusion-decellularization technique seem to be interesting in order to overcome these limitations. We present here an evaluation of the biomechanics of the bones thus obtained. MATERIAL AND METHODS: Two decellularization protocols were chosen for this study. One using Sodium Dodecyl Sulfate (SDS) (D1) and one using NaOH and H2O2 (D2). The decellularization was performed on porcine forearms. We then carried out compression, three-point bending, indentation and screw pull-out tests on each sample. Once these tests were completed, we compared the results obtained between the different decellularization protocols and with samples left native. RESULTS: The difference in the means was similar between the tests performed on bones decellularized with the SDS protocol and native bones for pull-out test: +1.4% (CI95% [-10.5%- 12.4%]) of mean differences when comparing Native vs D1, compression -14.9% (CI95% [-42.7%- 12.5%]), 3-point bending -5.7% (CI95% [-22.5%- 11.1%]) and indentation -10.8% (CI95% [-19.5%- 4.6%]). Bones decellularized with the NaOH protocol showed different results from those obtained with the SDS protocol or native bones during the pull-out screw +40.7% (CI95% [24.3%- 57%]) for Native vs D2 protocol and 3-point bending tests +39.2% (CI95% [13.7%- 64.6%]) for Native vs D2 protocol. The other tests, compression and indentation, gave similar results for all our samples. CONCLUSION: Vascularized decellularized grafts seem to be an interesting means for bone reconstruction. Our study shows that the decellularization method affects the mechanical results of our specimens. Some methods seem to limit these alterations and could be used in the future for bone decellularization.
Published: 2023

14. Prostacyclin receptor agonists induce DUSP1 to inhibit pulmonary artery smooth muscle cell proliferation.

Author: UCL - SSS/IREC/MONT - Pôle Mont Godinne, UCL - (MGD) Service de chirurgie cardio-vasculaire et thoracique, Maruyama, Hidekazu, Sakai, Satoshi, Dewachter, Laurence, Dewachter, Céline, Rondelet, Benoît, Naeije, Robert, Ieda, Masaki, UCL - SSS/IREC/MONT - Pôle Mont Godinne, UCL - (MGD) Service de chirurgie cardio-vasculaire et thoracique, Maruyama, Hidekazu, Sakai, Satoshi, Dewachter, Laurence, Dewachter, Céline, Rondelet, Benoît, Naeije, Robert, and Ieda, Masaki
Abstract: Upregulated p38 signaling is implicated in the accelerated proliferation of pulmonary artery smooth muscle cells (PA-SMCs) and the pathogenesis of pulmonary artery remodeling observed in pulmonary arterial hypertension (PAH). Previously, we reported that after endothelin-1 (ET-1) pretreatment, bone morphogenetic protein 2 (BMP2) activates p38 signaling and accelerates PA-SMC proliferation. The activity of p38 signaling is tightly regulated by the inactivation of dual-specificity phosphatase 1 (DUSP1). Activated p38 induces DUSP1 expression, forming a negative feedback loop. Prostacyclin IP receptor agonists (prostacyclin and selexipag) are used to treat PAH. In this study, we aimed to verify whether IP receptor agonists affect DUSP1 expression and accelerate the proliferation of PA-SMCs. PA-SMCs were treated with BMP2, ET-1, prostacyclin, and MRE-269, an active metabolite of selexipag, either alone or in combination. We quantified mRNA expressions using real-time quantitative polymerase chain reaction. Pulmonary artery specimens and PA-SMCs were obtained during lung transplantation in patients with PAH. Both prostacyclin and MRE-269 increased DUSP1 expression. Combined treatment with BMP2 and ET-1 induced cyclin D1 and DUSP1 expression and increased PA-SMC proliferation. MRE-269 attenuated BMP2/ET-1-induced cell proliferation. ET-1 increased DUSP1 expression in PA-SMCs from control patients but not in PA-SMCs from patients with PAH. This study showed that the p38/DUSP1 negative feedback loop is impaired in PAH, contributing to unregulated p38 activation and PA-SMC hyperplasia. IP receptor agonist MRE-269 increases DUSP1 expression and inhibit p38-mediated PA-SMC proliferation. Future elucidation of the detailed mechanism underlying reduced DUSP1 expression would be informative for PAH treatment.
Published: 2023

15. Systemic Inflammation/Nutritional Status Scores Are Prognostic but Not Predictive in Metastatic Non-Small-Cell Lung Cancer Treated with First-Line Immune Checkpoint Inhibitors

Author: UCL - SSS/IREC/MONT - Pôle Mont Godinne, UCL - SSS/IREC/PNEU - Pôle de Pneumologie, ORL et Dermatologie, UCL - (MGD) Unité de support scientifique, UCL - (MGD) Service de pneumologie, UCL - (MGD) Service de radiologie - résonance magnétique, UCL - (MGD) Service de chirurgie cardio-vasculaire et thoracique, UCL - (MGD) Service d'anatomie pathologique, UCL - (MGD) Service de médecine nucléaire, UCL - (MGD) Service de radiothérapie, Mahiat, Cédric, Bihin, Benoît, Duplaquet, Fabrice, Stanciu Pop, Claudia, Dupont, Michaël, Vander Borght, Thierry, Rondelet, Benoît, Vanderick, Jean, Andre, Bénédicte, Pirard, Lionel, Ocak, Sebahat, UCL - SSS/IREC/MONT - Pôle Mont Godinne, UCL - SSS/IREC/PNEU - Pôle de Pneumologie, ORL et Dermatologie, UCL - (MGD) Unité de support scientifique, UCL - (MGD) Service de pneumologie, UCL - (MGD) Service de radiologie - résonance magnétique, UCL - (MGD) Service de chirurgie cardio-vasculaire et thoracique, UCL - (MGD) Service d'anatomie pathologique, UCL - (MGD) Service de médecine nucléaire, UCL - (MGD) Service de radiothérapie, Mahiat, Cédric, Bihin, Benoît, Duplaquet, Fabrice, Stanciu Pop, Claudia, Dupont, Michaël, Vander Borght, Thierry, Rondelet, Benoît, Vanderick, Jean, Andre, Bénédicte, Pirard, Lionel, and Ocak, Sebahat
Abstract: Biomarkers of systemic inflammation/nutritional status have been associated with outcomes in advanced-stage non-small-cell lung cancer (NSCLC) treated with immune checkpoint inhibitors (ICIs). However, most of them were not tested in cohorts of patients treated with ICIs in combination with chemotherapy (CT) (ICI + CT) or with CT alone, making it impossible to discriminate a predictive from a prognostic effect. We conducted a single-center retrospective study to search for associations between various baseline biomarkers/scores that reflected the systemic inflammation/nutritional status (Lung Immune Prognostic Index, Modified Lung Immune Prognostic Index, Scottish Inflammatory Prognostic Score, Advanced Lung Cancer Inflammation Index, EPSILoN, Prognostic Nutritional Index, Systemic Immune-Inflammation Index, Gustave Roussy Immune Score, Royal Marsden Hospital Prognostic Score, Lung Immuno-oncology Prognostic Score 3, Lung Immuno-oncology Prognostic Score 4, score published by Holtzman et al., and Glasgow Prognostic Score) and outcomes in metastatic NSCLC treated in a first-line setting either with ICI in monotherapy (cohort 1; n = 75), ICI + CT (cohort 2; n = 56), or CT alone (cohort 3; n = 221). In the three cohorts, the biomarkers/scores were moderately associated with overall survival (OS) and progression-free survival (PFS). Their prognostic performance was relatively poor, with a maximum c-index of 0.66. None of them was specific to ICIs and could help to choose the best treatment modality. The systemic inflammation/nutritional status, associated with outcomes independently of the treatment, is therefore prognostic but not predictive in metastatic NSCLC.
Published: 2023

16. Evaluation of Myocardial Improvement in Patients Supported by Ventricular Assist Device Under Optimal Pharmacological Therapy

Author: Service de Chirurgie Cardiaque, Institut de Physiologie, Strasbourg, France, and Institut National de la Santé Et de la Recherche Médicale, France
Published: 2011

17. Anestesia-reanimación en cirugía del cáncer de esófago

Author: Capron, B., Le Frêche, H., Mariette, C., and Lebuffe, G.
Published: 2015
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18. Creation of bioengineered vascularized spleen matrix as an endocrine cell support

Author: UCL - SSS/IREC/CHEX - Pôle de chirgurgie expérimentale et transplantation, UCL - (SLuc) Service de chirurgie plastique, UCL - SSS/IREC/MORF - Pôle de Morphologie, UCL - SSS/IREC - Institut de recherche expérimentale et clinique, UCL - (SLuc) Service de chirurgie et transplantation abdominale, Maistriaux, Louis, Ramirez Acosta, Matias, Fievé, Lies, Chae, Hee-Young, Bouzin, Caroline, Bonaccorsi Riani, Eliano, Duisit, Jérôme, Gianello, Pierre, Behets Wydemans, Catherine, Lengelé, Benoît, UCL - SSS/IREC/CHEX - Pôle de chirgurgie expérimentale et transplantation, UCL - (SLuc) Service de chirurgie plastique, UCL - SSS/IREC/MORF - Pôle de Morphologie, UCL - SSS/IREC - Institut de recherche expérimentale et clinique, UCL - (SLuc) Service de chirurgie et transplantation abdominale, Maistriaux, Louis, Ramirez Acosta, Matias, Fievé, Lies, Chae, Hee-Young, Bouzin, Caroline, Bonaccorsi Riani, Eliano, Duisit, Jérôme, Gianello, Pierre, Behets Wydemans, Catherine, and Lengelé, Benoît
Published: 2021

19. Perfusion-decellularization of vascularized bone matrix: Application to the porcine forelimb

Author: UCL - SSS/IREC/MORF - Pôle de Morphologie, UCL - SSS/IREC/CHEX - Pôle de chirgurgie expérimentale et transplantation, UCL - (SLuc) Service de chirurgie plastique, UCL - SSS/IREC/NMSK - Neuro-musculo-skeletal Lab, UCL - SST/IMMC/MEED - Mechatronic, Electrical Energy, and Dynamics Systems, UCL - (SLuc) Service de chirurgie et transplantation abdominale, Rougier, G, Maistriaux, Louis, Fievé, Lies, Xhema, Daela, Manon, Julie, Evrard, Robin, de Ville de Goyet, Christine, Olszewski, Raphael, Kerckhofs, Greet, Szmytka, F, Thurieau, N, Boisson, J, Kadlub, N, Gianello, Pierre, Behets Wydemans, Catherine, Lengelé, Benoît, UCL - SSS/IREC/MORF - Pôle de Morphologie, UCL - SSS/IREC/CHEX - Pôle de chirgurgie expérimentale et transplantation, UCL - (SLuc) Service de chirurgie plastique, UCL - SSS/IREC/NMSK - Neuro-musculo-skeletal Lab, UCL - SST/IMMC/MEED - Mechatronic, Electrical Energy, and Dynamics Systems, UCL - (SLuc) Service de chirurgie et transplantation abdominale, Rougier, G, Maistriaux, Louis, Fievé, Lies, Xhema, Daela, Manon, Julie, Evrard, Robin, de Ville de Goyet, Christine, Olszewski, Raphael, Kerckhofs, Greet, Szmytka, F, Thurieau, N, Boisson, J, Kadlub, N, Gianello, Pierre, Behets Wydemans, Catherine, and Lengelé, Benoît
Published: 2021

20. Thoracic duct-azygos vein anastomosis in an infant with superior vena cava syndrome and recurrent chylothorax.

Author: UCL - (SLuc) Service de chirurgie cardiovasculaire et thoracique, UCL - SSS/IREC/MORF - Pôle de Morphologie, UCL - (SLuc) Centre du cancer, UCL - (SLuc) Service de chirurgie plastique, UCL - SSS/IREC/CARD - Pôle de recherche cardiovasculaire, de Beco, Geoffroy, Van Winghem, Joëlle, Lengelé, Benoît, Poncelet, Alain J, UCL - (SLuc) Service de chirurgie cardiovasculaire et thoracique, UCL - SSS/IREC/MORF - Pôle de Morphologie, UCL - (SLuc) Centre du cancer, UCL - (SLuc) Service de chirurgie plastique, UCL - SSS/IREC/CARD - Pôle de recherche cardiovasculaire, de Beco, Geoffroy, Van Winghem, Joëlle, Lengelé, Benoît, and Poncelet, Alain J
Abstract: In this study, we describe an intrathoracic microsurgical lymphatico-venous anastomosis as an alternative surgical technique for the treatment of refractory chylothorax in an infant. This procedure allowed us to restore enteral nutrition within days of surgery. At 3-year follow-up, there was no recurrence of pleural effusion.
Published: 2020

21. Esophageal Trachea, a Unique Foregut Malformation Requiring Multistage Surgical Reconstruction: Case Report.

Author: UCL - SSS/IREC - Institut de recherche expérimentale et clinique, UCL - SSS/IREC/PEDI - Pôle de Pédiatrie, UCL - SSS/IREC/CHEX - Pôle de chirgurgie expérimentale et transplantation, UCL - SSS/IREC/MIRO - Pôle d'imagerie moléculaire, radiothérapie et oncologie, UCL - (SLuc) Service de chirurgie et transplantation abdominale, UCL - (SLuc) Service de soins intensifs, UCL - (SLuc) Service de pédiatrie générale, UCL - (SLuc) Service de radiologie, UCL - (SLuc) Service d'oto-rhino-laryngologie, UCL - (SLuc) Service d'anesthésiologie, UCL - SSS/IREC/CARD - Pôle de recherche cardiovasculaire, UCL - (SLuc) Service de chirurgie cardiovasculaire et thoracique, UCL - (SLuc) Service de gastro-entérologie et hépatologie pédiatrique, Tambucci, Roberto, Wautelet, Océane, Haenecour, Astrid, François, Geneviève, Goubau, Christophe, Scheers, Isabelle, Halut, Marin, Menten, Renaud, Schmitz, Sandra, de Toeuf, Caroline, Pirotte, Thierry, D'hondt, Beelke, Reding, Raymond, Poncelet, Alain, UCL - SSS/IREC - Institut de recherche expérimentale et clinique, UCL - SSS/IREC/PEDI - Pôle de Pédiatrie, UCL - SSS/IREC/CHEX - Pôle de chirgurgie expérimentale et transplantation, UCL - SSS/IREC/MIRO - Pôle d'imagerie moléculaire, radiothérapie et oncologie, UCL - (SLuc) Service de chirurgie et transplantation abdominale, UCL - (SLuc) Service de soins intensifs, UCL - (SLuc) Service de pédiatrie générale, UCL - (SLuc) Service de radiologie, UCL - (SLuc) Service d'oto-rhino-laryngologie, UCL - (SLuc) Service d'anesthésiologie, UCL - SSS/IREC/CARD - Pôle de recherche cardiovasculaire, UCL - (SLuc) Service de chirurgie cardiovasculaire et thoracique, UCL - (SLuc) Service de gastro-entérologie et hépatologie pédiatrique, Tambucci, Roberto, Wautelet, Océane, Haenecour, Astrid, François, Geneviève, Goubau, Christophe, Scheers, Isabelle, Halut, Marin, Menten, Renaud, Schmitz, Sandra, de Toeuf, Caroline, Pirotte, Thierry, D'hondt, Beelke, Reding, Raymond, and Poncelet, Alain
Abstract: Abnormal connections between the esophagus and low respiratory tract can result from embryological defects in foregut development. Beyond well-known malformations, including tracheo-esophageal fistula and laryngo-tracheo-esophageal cleft, rarer anomalies have also been reported, including communicating bronchopulmonary foregut malformations and tracheal atresia. Herein, we describe a case of what we have called "esophageal trachea," which, to our knowledge, has yet to be reported. A full-term neonate was born in our institution presenting with a foregut malformation involving both the middle esophagus and the distal trachea, which were found to be longitudinally merged into a common segment, 3 cm in length, located just above the carina and consisted of esophageal tissue without cartilaginous rings. At birth, the esophagus and trachea were surgically separated via right thoracotomy, the common segment kept on the tracheal side only, creating a residual long-gap esophageal atresia. The resulting severe tracheomalacia was treated via simultaneous posterior splinting of such diseased segment using an autologous pericardium patch, as well as by anterior aortopexy. Terminal esophagostomy and gastrostomy were created at that stage due to the long distance between esophageal segments. Between ages 18 and 24 months, the patient underwent native esophageal reconstruction using a multistage traction-and-growth surgical strategy that combined Kimura extra-thoracic esophageal elongations at the upper esophagus and Foker external traction at the distal esophagus. Ten months after esophageal reconstruction, prolonged, refractory, and severe tracheomalacia was further treated via anterior external stenting using a semitubular ringed Gore-Tex® prosthesis, through simultaneous median sternotomy and tracheoscopy. Currently, 2 years after the last surgery, respiratory stabilization, and full oral feeding were stably achieved. Multidisciplinary management was crucial for assuring lifes
Published: 2020

22. Thoracic duct-azygos vein anastomosis in an infant with superior vena cava syndrome and recurrent chylothorax.

Author: UCL - SSS/IREC/CARD - Pôle de recherche cardiovasculaire, UCL - (SLuc) Service de chirurgie cardiovasculaire et thoracique, UCL - (SLuc) Service de pédiatrie générale, UCL - SSS/IREC/MORF - Pôle de Morphologie, UCL - (SLuc) Service de chirurgie plastique, de Beco, Geoffroy, Van Winghem, Joëlle, Lengele, Benoit, Poncelet, Alain, UCL - SSS/IREC/CARD - Pôle de recherche cardiovasculaire, UCL - (SLuc) Service de chirurgie cardiovasculaire et thoracique, UCL - (SLuc) Service de pédiatrie générale, UCL - SSS/IREC/MORF - Pôle de Morphologie, UCL - (SLuc) Service de chirurgie plastique, de Beco, Geoffroy, Van Winghem, Joëlle, Lengele, Benoit, and Poncelet, Alain
Abstract: In this study, we describe an intrathoracic microsurgical lymphatico-venous anastomosis as an alternative surgical technique for the treatment of refractory chylothorax in an infant. This procedure allowed us to restore enteral nutrition within days of surgery. At 3-year follow-up, there was no recurrence of pleural effusion.
Published: 2020

23. Simultaneous nephrectomy during kidney transplantation for polycystic kidney disease does not detrimentally impact comorbidity and graft survival

Author: UCL - SSS/IREC/CHEX - Pôle de chirgurgie expérimentale et transplantation, UCL - (SLuc) Service de chirurgie et transplantation abdominale, UCL - SSS/IREC/NEFR - Pôle de Néphrologie, UCL - (SLuc) Service de néphrologie, Darius, Tom, Bertoni, Sébastien, De Meyer, Martine, Buemi, Antoine, Devresse, Arnaud, Kanaan, Nada, Goffin, Eric, Mourad, Michel, UCL - SSS/IREC/CHEX - Pôle de chirgurgie expérimentale et transplantation, UCL - (SLuc) Service de chirurgie et transplantation abdominale, UCL - SSS/IREC/NEFR - Pôle de Néphrologie, UCL - (SLuc) Service de néphrologie, Darius, Tom, Bertoni, Sébastien, De Meyer, Martine, Buemi, Antoine, Devresse, Arnaud, Kanaan, Nada, Goffin, Eric, and Mourad, Michel
Published: 2022

24. Humoral response to SARS-CoV-2 infection among liver transplant recipients

Author: UCL - SSS/IREC/CHEX - Pôle de chirgurgie expérimentale et transplantation, UCL - SSS/IREC - Institut de recherche expérimentale et clinique, UCL - (SLuc) Service de chirurgie et transplantation abdominale, UCL - (SLuc) Service de gastro-entérologie, Becchetti, Chiara, Broekhoven, Annelotte G C, Dahlqvist, Géraldine, Fraga, Montserrat, Zambelli, Marco Fabrizio, Ciccarelli, Olga, Saouli, Anne-Catherine, Trizzino, Arianna, Banz, Vanessa, Dufour, Jean-François, Roukens, Anna H E, Torres Morales, Shessy P, Myeni, Sebenzile K, Kikkert, Marjolein, Feltkamp, Mariet C W, Coenraad, Minneke J, UCL - SSS/IREC/CHEX - Pôle de chirgurgie expérimentale et transplantation, UCL - SSS/IREC - Institut de recherche expérimentale et clinique, UCL - (SLuc) Service de chirurgie et transplantation abdominale, UCL - (SLuc) Service de gastro-entérologie, Becchetti, Chiara, Broekhoven, Annelotte G C, Dahlqvist, Géraldine, Fraga, Montserrat, Zambelli, Marco Fabrizio, Ciccarelli, Olga, Saouli, Anne-Catherine, Trizzino, Arianna, Banz, Vanessa, Dufour, Jean-François, Roukens, Anna H E, Torres Morales, Shessy P, Myeni, Sebenzile K, Kikkert, Marjolein, Feltkamp, Mariet C W, and Coenraad, Minneke J
Abstract: Objective Immunosuppressive agents are known to interfere with T and/or B lymphocytes, which are required to mount an adequate serologic response. Therefore, we aim to investigate the antibody response to SARS-CoV- 2 in liver transplant (LT) recipients after COVID-19. Design Prospective multicentre case–control study, analysing antibodies against the nucleocapsid protein, spike (S) protein of SARS-CoV- 2 and their neutralising activity in LT recipients with confirmed SARS-CoV- 2 infection (COVID-19- LT) compared with immunocompetent patients (COVID-19- immunocompetent) and LT recipients without COVID-19 symptoms (non-COVID- 19- LT). Results Overall, 35 LT recipients were included in the COVID-19- LT cohort. 35 and 70 subjects fulfilling the matching criteria were assigned to the COVID-19- immunocompetent and non-COVID- 19- LT cohorts, respectively. We showed that LT recipients, despite immunosuppression and less symptoms, mounted a detectable antinucleocapsid antibody titre in 80% of the cases, although significantly lower compared with the COVID-19- immunocompetent cohort (3.73 vs 7.36 index level, p<0.001). When analysing anti-S antibody response, no difference in positivity rate was found between the COVID-19- LT and COVID-19- immunocompetent cohorts (97.1% vs 100%, p=0.314). Functional antibody testing showed neutralising activity in 82.9% of LT recipients (vs 100% in COVID-19- immunocompetent cohort, p=0.024). Conclusions Our findings suggest that the humoral response of LT recipients is only slightly lower than expected, compared with COVID-19 immunocompetent controls. Testing for anti-S antibodies alone can lead to an overestimation of the neutralising ability in LT recipients. Altogether, routine antibody testing against separate SARS-CoV- 2 antigens and functional testing show that the far majority of LT patients are capable of mounting an adequate antibody response with neutralising ability.
Published: 2022

25. Anesthesia and World War II: When the Battlefield Becomes a Research Field-A Bibliometric Analysis of the Influence of World War II on the Development of Anesthesiology.

Author: UCL - SSS/IREC/NMSK - Neuro-musculo-skeletal Lab, UCL - SSS/IREC/CHEX - Pôle de chirgurgie expérimentale et transplantation, UCL - (SLuc) Service d'orthopédie et de traumatologie de l'appareil locomoteur, UCL - (SLuc) Service de chirurgie et transplantation abdominale, Jakus, Lien, Docquier, Pierre-Louis, Veyckemans, Francis, Reding, Raymond, UCL - SSS/IREC/NMSK - Neuro-musculo-skeletal Lab, UCL - SSS/IREC/CHEX - Pôle de chirgurgie expérimentale et transplantation, UCL - (SLuc) Service d'orthopédie et de traumatologie de l'appareil locomoteur, UCL - (SLuc) Service de chirurgie et transplantation abdominale, Jakus, Lien, Docquier, Pierre-Louis, Veyckemans, Francis, and Reding, Raymond
Abstract: At the outbreak of World War II (WWII), anesthesiology was struggling to establish itself as a medical specialty. The battlefield abruptly exposed this young specialty to the formidable challenge of mass casualties, with an urgent need to provide proper fluid resuscitation, airway management, mechanical ventilation, and analgesia to thousands. But while Europe was suffering under the Nazi boot, anesthesia was preparing to rise to the challenge posed by the impending war. While war brings death and destruction, it also opens the way to medical advances. The aim of this study is to measure the evolution of anesthesia owing to WWII. We conducted a retrospective observational bibliometric study involving a quantitative and statistical analysis of publications. The following 7 journals were selected to cover European and North American anesthesia-related publications: Anesthesia & Analgesia, the British Journal of Anaesthesia, Anesthesiology, Schmerz-Narkose-Anaesthesie, Surgery, La Presse Médicale, and The Military Surgeon (later Military Medicine). Attention was focused on journal volumes published between 1920 and 1965. After reviewing the literature, we selected 12 keywords representing important advances in anesthesiology since 1920: "anesthesia," "balanced anesthesia," "barbiturates," "d-tubocurarine," "endotracheal intubation," "ether," "lidocaine," "morphine," "spinal anesthesia," "thiopental," "transfusion," and "trichloroethylene." Titles of original articles from all selected journals editions between 1920 and 1965 were screened for the occurrence of 1 of the 12 keywords. A total of 26,132 original article titles were screened for the occurrence of the keywords. A total of 1815 keywords were found. Whereas Anesthesia & Analgesia had the highest keyword occurrence (493 citations), Schmerz-Narkose-Anaesthesie had the lowest (38 citations). The number of publications of the 12 keywords was significantly higher in the postwar than in the prewar period (65% and 35%
Published: 2022

26. Effective multimodal management of a giant adrenocortical carcinoma.

Author: UCL - SSS/IREC/GAEN - Pôle d'Hépato-gastro-entérologie, UCL - SSS/IREC/MONT - Pôle Mont Godinne, UCL - SSS/IREC/EDIN - Pôle d'endocrinologie, diabète et nutrition, UCL - (MGD) Service d'endocrinologie, UCL - (MGD) Service de chirurgie, UCL - (MGD) Service d'oncologie médicale, UCL - (MGD) Service d'anatomie pathologique, Pairon, Camille, Dili, Alexandra, Claude, Bertrand, D'Hondt, Lionel, Fervaille, Caroline, Donckier, Julian, UCL - SSS/IREC/GAEN - Pôle d'Hépato-gastro-entérologie, UCL - SSS/IREC/MONT - Pôle Mont Godinne, UCL - SSS/IREC/EDIN - Pôle d'endocrinologie, diabète et nutrition, UCL - (MGD) Service d'endocrinologie, UCL - (MGD) Service de chirurgie, UCL - (MGD) Service d'oncologie médicale, UCL - (MGD) Service d'anatomie pathologique, Pairon, Camille, Dili, Alexandra, Claude, Bertrand, D'Hondt, Lionel, Fervaille, Caroline, and Donckier, Julian
Abstract: Adrenocortical carcinoma is a rare and aggressive tumour. The only curative treatment is surgery with negative margins. In most series, the average lesion size ranges from 5.5 to 15 cm. We report the case of a 27-year-old female with hyperandrogenism and Cushing syndrome due to a right adrenocortical carcinoma of 19.7 cm. The tumour abutting on liver and vena cava and the presence two nodules in liver required extensive surgery including a right posterior sectionectomy and an en bloc resection of the adrenal mass together with the right kidney and the gallbladder. The vena cava was also resected with a reconstruction using a pericardial patch since it was invaded on its border. Pathological examination confirmed an adrenocortical carcinoma, with tumour invasion of vessels, tumour capsule, vena cava and two metastases in the liver (pT4N0M1). All margins were negative. Three months after surgery, two lung nodules, cardio-phrenic and internal mammary adenomegalies were noticed on a PET/CT scan, justifying the initiation of chemotherapy, alongside with mitotane. After a 10-month follow-up, CT scan was stable excepted for a lung nodule growing from 4 to 7 mm. Targeted stereotaxic radiotherapy was then administered. Twenty-two months after surgery, the patient has improved considerably and all signs of hyperandrogenism and Cushing syndrome have resolved. This case of adrenocortical carcinoma illustrates one of the largest tumours among those reported. It demonstrates the feasibility and effectiveness of a multimodal approach in its treatment even if it is giant and at high risk.
Published: 2022

27. Trying To Enhance Massive Bone Allografts Outcomes By A New Perfusion/Decellularization Protocol

Author: UCL - SSS/IREC/CHEX - Pôle de chirgurgie expérimentale et transplantation, UCL - SSS/IREC/MORF - Pôle de Morphologie, UCL - SSS/IREC/NMSK - Neuro-musculo-skeletal Lab, UCL - (SLuc) Service d'orthopédie et de traumatologie de l'appareil locomoteur, UCL - (SLuc) Service de chirurgie plastique, UCL - (SLuc) Service des urgences, Evrard, Robin, Maistriaux, Louis, Manon, Julie, Rafferty, Chiara, Cornu, Olivier, Lengelé, Benoît, Schubert, Thomas, UCL - SSS/IREC/CHEX - Pôle de chirgurgie expérimentale et transplantation, UCL - SSS/IREC/MORF - Pôle de Morphologie, UCL - SSS/IREC/NMSK - Neuro-musculo-skeletal Lab, UCL - (SLuc) Service d'orthopédie et de traumatologie de l'appareil locomoteur, UCL - (SLuc) Service de chirurgie plastique, UCL - (SLuc) Service des urgences, Evrard, Robin, Maistriaux, Louis, Manon, Julie, Rafferty, Chiara, Cornu, Olivier, Lengelé, Benoît, and Schubert, Thomas
Published: 2022

28. Fibrin-based factor delivery for therapeutic angiogenesis: friend or foe?

Author: UCL - (MGD) Service de chirurgie cardio-vasculaire et thoracique, UCL - SSS/IREC/MONT - Pôle Mont Godinne, Melly, Ludovic, Banfi, Andrea, UCL - (MGD) Service de chirurgie cardio-vasculaire et thoracique, UCL - SSS/IREC/MONT - Pôle Mont Godinne, Melly, Ludovic, and Banfi, Andrea
Abstract: Therapeutic angiogenesis aims at promoting the growth of blood vessels to restore perfusion in ischemic tissues or aid tissue regeneration. Vascular endothelial growth factor (VEGF) is the master regulator of angiogenesis in development, repair, and disease. However, exploiting VEGF for therapeutic purposes has been challenging and needs to take into account some key aspects of VEGF biology. In particular, the spatial localization of angiogenic signals within the extracellular matrix is crucial for physiological assembly and function of new blood vessels. Fibrin is the provisional matrix that is universally deposited immediately after injury and supports the initial steps of tissue regeneration. It provides therefore several ideal features as a substrate to promote therapeutic vascularization, especially through its ability to present growth factors in their physiological matrix-bound state and to modulate their availability for signaling. Here, we provide an overview of fibrin uses as a tissue-engineering scaffold material and as a tunable platform to finely control dose and duration of delivery of recombinant factors in therapeutic angiogenesis. However, in some cases, fibrin has also been associated with undesirable outcomes, namely the promotion of fibrosis and scar formation that actually prevent physiological tissue regeneration. Understanding the mechanisms that tip the balance between the pro- and anti-regenerative functions of fibrin will be the key to fully exploit its therapeutic potential.
Published: 2022

29. Prognostic effect of body mass index in patients with advanced NSCLC treated with chemoimmunotherapy combinations.

Author: UCL - SSS/IREC/CARD - Pôle de recherche cardiovasculaire, UCL - SSS/IREC/PNEU - Pôle de Pneumologie, ORL et Dermatologie, UCL - SSS/IREC/SLUC - Pôle St.-Luc, UCL - (SLuc) Service d'anatomie pathologique, UCL - (SLuc) Service de chirurgie cardiovasculaire et thoracique, UCL - (SLuc) Service de pneumologie, Cortellini, Alessio, Ricciuti, Biagio, Vaz, Victor R, Soldato, Davide, Alessi, Joao V, Dall'Olio, Filippo G, Banna, Giuseppe L, Muthuramalingam, Sethupathi, Chan, Samuel, Majem, Margarita, Piedra, Aida, Lamberti, Giuseppe, Andrini, Elisa, Addeo, Alfredo, Friedlaender, Alex, Facchinetti, Francesco, Gorría, Teresa, Mezquita, Laura, Hoton, Delphine, Lacroix, Valerie, Aboubakar Nana, Frank, Artingstall, James, Comins, Charles, Di Maio, Massimo, Caglio, Andrea, Cave, Judith, McKenzie, Hayley, Newsom-Davis, Thomas, Evans, Joanne S, Tiseo, Marcello, D'Alessio, Antonio, Fulgenzi, Claudia A M, Besse, Benjamin, Awad, Mark M, Pinato, David J, UCL - SSS/IREC/CARD - Pôle de recherche cardiovasculaire, UCL - SSS/IREC/PNEU - Pôle de Pneumologie, ORL et Dermatologie, UCL - SSS/IREC/SLUC - Pôle St.-Luc, UCL - (SLuc) Service d'anatomie pathologique, UCL - (SLuc) Service de chirurgie cardiovasculaire et thoracique, UCL - (SLuc) Service de pneumologie, Cortellini, Alessio, Ricciuti, Biagio, Vaz, Victor R, Soldato, Davide, Alessi, Joao V, Dall'Olio, Filippo G, Banna, Giuseppe L, Muthuramalingam, Sethupathi, Chan, Samuel, Majem, Margarita, Piedra, Aida, Lamberti, Giuseppe, Andrini, Elisa, Addeo, Alfredo, Friedlaender, Alex, Facchinetti, Francesco, Gorría, Teresa, Mezquita, Laura, Hoton, Delphine, Lacroix, Valerie, Aboubakar Nana, Frank, Artingstall, James, Comins, Charles, Di Maio, Massimo, Caglio, Andrea, Cave, Judith, McKenzie, Hayley, Newsom-Davis, Thomas, Evans, Joanne S, Tiseo, Marcello, D'Alessio, Antonio, Fulgenzi, Claudia A M, Besse, Benjamin, Awad, Mark M, and Pinato, David J
Abstract: INTRODUCTION: It has been recognized that increasing body mass index (BMI) is associated with improved outcome from immune checkpoint inhibitors (ICIs) in patients with various malignancies including non-small cell lung cancer (NSCLC). However, it is unclear whether baseline BMI may influence outcomes from first-line chemoimmunotherapy combinations. METHODS: In this international multicenter study, we evaluated the association between baseline BMI, progression-free survival (PFS) and overall survival (OS) in a cohort of patients with stage IV NSCLC consecutively treated with first-line chemoimmunotherapy combinations. BMI was categorized according to WHO criteria. Results: Among the 853 included patients, 5.3% were underweight; 46.4% were of normal weight; 33.8% were overweight; and 14.5% were obese. Overweight and obese patients were more likely aged ≥70 years (p=0.00085), never smokers (p<0.0001), with better baseline Eastern Cooperative Oncology Group-Performance Status (p=0.0127), and had lower prevalence of central nervous system (p=0.0002) and liver metastases (p=0.0395). Univariable analyses showed a significant difference in the median OS across underweight (15.5 months), normal weight (14.6 months), overweight (20.9 months), and obese (16.8 months) patients (log-rank: p=0.045, log rank test for trend: p=0.131), while no difference was found with respect to the median PFS (log-rank for trend: p=0.510). Neither OS nor PFS was significantly associated with baseline BMI on multivariable analysis. CONCLUSIONS: In contrast to what was observed in the context of chemotherapy-free ICI-based regimens, baseline BMI does not affect clinical outcomes from chemoimmunotherapy combinations in patients with advanced NSCLC.
Published: 2022

30. Pregnancy Outcomes After Kidney Transplantation and Long-Term Evolution of Children: A Single Center Experience

Author: UCL - SSS/IREC - Institut de recherche expérimentale et clinique, UCL - SSS/IREC/CHEX - Pôle de chirgurgie expérimentale et transplantation, UCL - SSS/IREC/NEFR - Pôle de Néphrologie, UCL - (SLuc) Service de néphrologie, UCL - (SLuc) Service d'obstétrique, UCL - (SLuc) Service de chirurgie et transplantation abdominale, Devresse, Arnaud, Jassogne, Carole, Hubinont, Corinne, Debieve, Frédéric, De Meyer, Martine, Mourad, Michel, Darius, Tom, Buemi, Antoine, Goffin, Eric, Kanaan, Nada, UCL - SSS/IREC - Institut de recherche expérimentale et clinique, UCL - SSS/IREC/CHEX - Pôle de chirgurgie expérimentale et transplantation, UCL - SSS/IREC/NEFR - Pôle de Néphrologie, UCL - (SLuc) Service de néphrologie, UCL - (SLuc) Service d'obstétrique, UCL - (SLuc) Service de chirurgie et transplantation abdominale, Devresse, Arnaud, Jassogne, Carole, Hubinont, Corinne, Debieve, Frédéric, De Meyer, Martine, Mourad, Michel, Darius, Tom, Buemi, Antoine, Goffin, Eric, and Kanaan, Nada
Abstract: Background. Pregnancies in women who underwent kidney transplants are at high risk compared with the general population. Methods. In this study, we aimed to retrospectively assess the obstetrical complications, delivery outcomes, and impact of pregnancy on kidney allograft function in a single-center cohort of kidney transplant recipients (KTRs). We provide data regarding the long-term evolution of children. Results. Thirty-two KTRs underwent a total of 57 pregnancies between 1994 and 2010. Fourteen pregnancies (24 %) did not survive caused by miscarriages (n = 9), stillborn (n = 1), ectopic pregnancies (n = 2), and medical abortion (n = 2). Live birth occurred in 76% of pregnancies. Delivery was by cesarean in 66%. The mean gestational age was 30.45 § 11.3 weeks and 65% of newborns were premature. A low birth weight <2500g was noted in 46%. Obstetric complications were de novo hypertension in 4%, pre-eclampsia in 9%, and gestational diabetes in 2%. The 5- and 10-year post-delivery death-censored graft loss rates were 3.1% and 12.5%, respectively. Data on 21 children were collected via a self-questionnaire. After a median follow-up time of 17 years, they appeared in good medical and psychological health. None of them suffered from chronic disease (especially uronephrological condition) or was taking chronic medication. Conclusions. Long-term evolution of children born to women who underwent kidney transplants seems favorable. Pregnancies in KTRs are successful in two-thirds of cases but are at increased risk of prematurity, delivery by cesarean, and low birth weight.
Published: 2022

31. Phage therapy to allow liver transplantation in a toddler infected by an extensively drug-resistant Pseudomonas aeruginosa

Author: UCL - SSS/IREC/PEDI - Pôle de Pédiatrie, UCL - SSS/IREC/CHEX - Pôle de chirgurgie expérimentale et transplantation, UCL - (SLuc) Service de gastro-entérologie et hépatologie pédiatrique, UCL - (SLuc) Service de pédiatrie générale, UCL - (SLuc) Service de chirurgie et transplantation abdominale, Van Nieuwenhuyse, Brieuc, van Der Linden, Dimitri, Chatzis, Olga, Lood, Cedric, Wagemans, Jeroen, Lavigne, Rob, de Magnee, Catherine, Sokal, Etienne, Rodriguez-Villalobos, Hector, Djebara, Sarah, Soentjens, Patrick, Pirnay, Jean-Paul, International Pediatric Transplant Association, UCL - SSS/IREC/PEDI - Pôle de Pédiatrie, UCL - SSS/IREC/CHEX - Pôle de chirgurgie expérimentale et transplantation, UCL - (SLuc) Service de gastro-entérologie et hépatologie pédiatrique, UCL - (SLuc) Service de pédiatrie générale, UCL - (SLuc) Service de chirurgie et transplantation abdominale, Van Nieuwenhuyse, Brieuc, van Der Linden, Dimitri, Chatzis, Olga, Lood, Cedric, Wagemans, Jeroen, Lavigne, Rob, de Magnee, Catherine, Sokal, Etienne, Rodriguez-Villalobos, Hector, Djebara, Sarah, Soentjens, Patrick, Pirnay, Jean-Paul, and International Pediatric Transplant Association
Abstract: Brieuc Van Nieuwenhuyse1, Dimitri Van der Linden1,2, Olga Chatzis2, Cédric Lood3,4, Jeroen Wagemans3, Rob Lavigne4, Catherine de Magnée5, Étienne Sokal1,6, Hector Rodriguez-Villalobos7, Sarah Djebara8, Maya Merabishvili9, Patrick Soentjens8, Jean-Paul Pirnay9. 1Institute of Experimental and Clinical Research's Pediatric department, UCLouvain, Brussels, Belgium; 2Pediatric Infectious Diseases, General Pediatrics Department, Cliniques universitaires Saint-Luc, Brussels, Belgium; 3Department of Biosystems, Laboratory of Gene Technology, KULeuven, Leuven, Belgium; 4Department of Microbial and Molecular Systems, Centre of Microbial and Plant Genetics, KULeuven, Leuven, Belgium; 5Pediatric and Transplantation Surgery, Cliniques universitaires Saint-Luc, Brussels, Belgium; 6Pediatric Hepatology and Gastro-enterology, Cliniques universitaires Saint-Luc, Brussels, Belgium; 7Department of Microbiology, Cliniques universitaires Saint-Luc, Brussels, Belgium; 8Center for Infectious Diseases, Queen Astrid Military Hospital, Brussels, Belgium; 9Laboratory for Molecular and Cellular Technology, Queen Astrid Military Hospital, Brussels, Belgium A 14-month old boy undergoes a first liver transplantation (LT) (Day 0), from an ABO-incompatible living donor. On D+20, we detect a fecal carriage of an extensively drug-resistant (XDR) Pseudomonas aeruginosa (Pa) strain. Besides intermediate susceptibility to aztreonam and colistin and susceptibility to gentamycin, the strain is resistant to all other antibiotics. On D+53, the child enters a severe septic state due to a bacteremia with the same Pa strain. New antibiogram suggests a resistance to colistin. Liver bilomas' drainage material is cultured and grows the same Pa strain. Admission to the pediatric intensive care unit and adjunction of intravenous (IV) aztreonam, gentamycin, and colistin led to no improvement on the microbiological or clinical levels during the next four days. By collaborating with Queen Astrid Military Hospital (Bru
Published: 2022

32. Corrigendum: Robotic Cardiac Surgery in Europe: Status 2020.

Author: UCL - SSS/IREC/MONT - Pôle Mont Godinne, UCL - (MGD) Service de chirurgie cardio-vasculaire et thoracique, Cerny, Stepan, Oosterlinck, Wouter, Onan, Burak, Singh, Sandeep, Segers, Patrique, Bolcal, Cengiz, Alhan, Cem, Navarra, Emiliano, Pettinari, Matteo, Van Praet, Frank, De Praetere, Herbert, Vojacek, Jan, Cebotaru, Theodor, Modi, Paul, Doguet, Fabien, Franke, Ulrich, Ouda, Ahmed, Melly, Ludovic, Malapert, Ghislain, Labrousse, Louis, Gianoli, Monica, Agnino, Alfonso, Philipsen, Tine, Jansens, Jean-Luc, Folliguet, Thierry, Palmen, Meindert, Pereda, Daniel, Musumeci, Francesco, Suwalski, Piotr, Cathenis, Koen, Van den Eynde, Jef, Bonatti, Johannes, UCL - SSS/IREC/MONT - Pôle Mont Godinne, UCL - (MGD) Service de chirurgie cardio-vasculaire et thoracique, Cerny, Stepan, Oosterlinck, Wouter, Onan, Burak, Singh, Sandeep, Segers, Patrique, Bolcal, Cengiz, Alhan, Cem, Navarra, Emiliano, Pettinari, Matteo, Van Praet, Frank, De Praetere, Herbert, Vojacek, Jan, Cebotaru, Theodor, Modi, Paul, Doguet, Fabien, Franke, Ulrich, Ouda, Ahmed, Melly, Ludovic, Malapert, Ghislain, Labrousse, Louis, Gianoli, Monica, Agnino, Alfonso, Philipsen, Tine, Jansens, Jean-Luc, Folliguet, Thierry, Palmen, Meindert, Pereda, Daniel, Musumeci, Francesco, Suwalski, Piotr, Cathenis, Koen, Van den Eynde, Jef, and Bonatti, Johannes
Abstract: [This corrects the article DOI: 10.3389/fcvm.2021.827515.].
Published: 2022

33. Monoclonal Antibody Therapy in Kidney Transplant Recipients With Delta and Omicron Variants of SARS-CoV-2: A Single-Center Case Series.

Author: UCL - SSS/IREC - Institut de recherche expérimentale et clinique, UCL - SSS/IREC/LTAP - Louvain Centre for Toxicology and Applied Pharmacology, UCL - SSS/IREC/CHEX - Pôle de chirgurgie expérimentale et transplantation, UCL - SSS/IREC/MBLG - Pôle de Microbiologie médicale, UCL - SSS/IREC/NEFR - Pôle de Néphrologie, UCL - (SLuc) Service de néphrologie, UCL - (SLuc) Service de microbiologie, UCL - (SLuc) Service de médecine interne générale, UCL - (SLuc) Service de chirurgie et transplantation abdominale, Fernandes, Guillaume, Devresse, Arnaud, Scohy, Anaïs, De Greef, Julien, Yombi, Jean Cyr, Belkhir, Leïla, Darius, Tom, Mourad, Michel, Buemi, Antoine, Kabamba Mukadi, Benoît, Goffin, Eric, Kanaan, Nada, UCL - SSS/IREC - Institut de recherche expérimentale et clinique, UCL - SSS/IREC/LTAP - Louvain Centre for Toxicology and Applied Pharmacology, UCL - SSS/IREC/CHEX - Pôle de chirgurgie expérimentale et transplantation, UCL - SSS/IREC/MBLG - Pôle de Microbiologie médicale, UCL - SSS/IREC/NEFR - Pôle de Néphrologie, UCL - (SLuc) Service de néphrologie, UCL - (SLuc) Service de microbiologie, UCL - (SLuc) Service de médecine interne générale, UCL - (SLuc) Service de chirurgie et transplantation abdominale, Fernandes, Guillaume, Devresse, Arnaud, Scohy, Anaïs, De Greef, Julien, Yombi, Jean Cyr, Belkhir, Leïla, Darius, Tom, Mourad, Michel, Buemi, Antoine, Kabamba Mukadi, Benoît, Goffin, Eric, and Kanaan, Nada
Abstract: Neutralizing monoclonal antibody treatments have shown promising preliminary results in kidney transplant recipients infected with SARS-CoV-2. However, their efficacy in kidney transplant recipients infected with Omicron variant has not been reported yet. Single-center retrospective study. We included all consecutive kidney transplant recipients treated with monoclonal antibodies for SARS-CoV-2 infection (positive polymerase chain reaction on nasopharyngeal swab) between June 10 2021 and January 14 2022. Forty-seven kidney transplant recipients were included. All patients had symptoms evolving for ≤ 7 days and no oxygen therapy need at monoclonal antibody infusion. Symptoms at diagnosis were mainly cough (n=25 [53%]) and fever (n=15 [32%]). Eighty-three percent of the cohort (n=39) had been vaccinated with at least 2 doses before infection, of whom 77% (n=30) had demonstrated a vaccine-induced humoral response. They were treated with either casirivimab-imdevimab (n= 16 [34%]) or sotrovimab (n= 31 [66%]) a median of 2 (ranges 0-6) days after the onset of symptoms. Except for one mild allergic reaction during casirivimab-imdevimab infusion, no side effects were reported. Median viral loads at admission (day 0) and 7 days after mAb infusion were 2,110,027 (ranges 1000-153,798,962) copies/mL and 1,000 (ranges 0-10,000,000) copies/mL, respectively. Genotypes were available for 22 kidney transplant recipients (47%). Omicron, Delta, and Gamma variants were identified in 13 (59%), 8 (36%), 1 (5%) patients, respectively. In kidney transplant recipients infected with Omicron variant, the median viral loads at day 0 and day 7 were 752,789 (ranges 4000-12,859,300) copies/mL and 1,353 (ranges 0-1,211,163) copies/mL, respectively. Two kidney transplant recipients required hospitalization immediately after sotrovimab perfusion for oxygen therapy that was weaned in 3 days allowing patients' discharge. None were admitted to the intensive care unit or died. Small sample-size, no cont
Published: 2022

34. Long-term outcome of liver transplantation for unresectable liver metastases from Neuroendocrine neoplasms: a Belgian retrospective multi-centre study

Author: UCL - SSS/IREC/CHEX - Pôle de chirgurgie expérimentale et transplantation, UCL - (SLuc) Service de chirurgie et transplantation abdominale, UCL - (SLuc) Service de gastro-entérologie, UCL - SSS/IREC - Institut de recherche expérimentale et clinique, UCL - SSS/IREC/GAEN - Pôle d'Hépato-gastro-entérologie, Bonaccorsi Riani, Eliano, Pulido Cloquell, Ignacio, Detry, O, Meurisse, N, Ysebaert, D, Pirenne, J, Verslype, C, Berrevoet, F, Vanlander, A, Lucidi, V, Coubeau, Laurent, Dahlqvist, Géraldine, Ciccarelli, Olga, Borbath, Ivan, UCL - SSS/IREC/CHEX - Pôle de chirgurgie expérimentale et transplantation, UCL - (SLuc) Service de chirurgie et transplantation abdominale, UCL - (SLuc) Service de gastro-entérologie, UCL - SSS/IREC - Institut de recherche expérimentale et clinique, UCL - SSS/IREC/GAEN - Pôle d'Hépato-gastro-entérologie, Bonaccorsi Riani, Eliano, Pulido Cloquell, Ignacio, Detry, O, Meurisse, N, Ysebaert, D, Pirenne, J, Verslype, C, Berrevoet, F, Vanlander, A, Lucidi, V, Coubeau, Laurent, Dahlqvist, Géraldine, Ciccarelli, Olga, and Borbath, Ivan
Published: 2022

35. Two miRNAs enriched in plasma extracellular vesicles are potential biomarkers for thyroid cancer.

Author: UCL - SSS/DDUV/CELL - Biologie cellulaire, UCL - SSS/IREC/FATH - Pôle de Pharmacologie et thérapeutique, UCL - SSS/IREC/MORF - Pôle de Morphologie, UCL - SSS/IONS - Institute of NeuroScience, UCL - SSS/DDUV - Institut de Duve, UCL - SSS/IREC/CHEX - Pôle de chirgurgie expérimentale et transplantation, UCL - (SLuc) Service de chirurgie et transplantation abdominale, Delcorte, Ophélie, Craps, Julie, Mahibullah, Siam, Spourquet, Catherine, D'Auria, Ludovic, Van Der Smissen, Patrick, Dessy, Chantal, Marbaix, Etienne, Mourad, Michel, Pierreux, Christophe, UCL - SSS/DDUV/CELL - Biologie cellulaire, UCL - SSS/IREC/FATH - Pôle de Pharmacologie et thérapeutique, UCL - SSS/IREC/MORF - Pôle de Morphologie, UCL - SSS/IONS - Institute of NeuroScience, UCL - SSS/DDUV - Institut de Duve, UCL - SSS/IREC/CHEX - Pôle de chirgurgie expérimentale et transplantation, UCL - (SLuc) Service de chirurgie et transplantation abdominale, Delcorte, Ophélie, Craps, Julie, Mahibullah, Siam, Spourquet, Catherine, D'Auria, Ludovic, Van Der Smissen, Patrick, Dessy, Chantal, Marbaix, Etienne, Mourad, Michel, and Pierreux, Christophe
Abstract: Differential diagnosis of thyroid cancer and benign nodules is still one of the most challenging issues in the field of endocrinology. To overcome overdiagnosis of papillary thyroid carcinomas (PTC) and the consecutive overtreatment of multinodular diseases, the search for easily accessible, sensitive and accurate biomarkers is critical. Several micro-RNAs (miRNAs) freely circulating in peripheral blood or enclosed in extracellular vesicles (EVs) have been proposed as potential biomarkers from non-invasive liquid biopsies. However, protocols are rarely comparable and conflicting data exists in the literature. In this work, we aimed to assess the diagnostic value of 6 micro-RNAs by comparing their expression in thyroid tissue to their abundance in bulk plasma and in plasma-EVs, before and after thyroid surgery. Plasma-EVs were isolated using a sequential density- and size-based fractionation, followed by in-depth characterization, confirming EV purity. Micro-RNA levels were measured by RT-qPCR in thyroid tissue, plasma and plasma-EVs. Among the 6 candidates, only miR-146b-5p and miR-21a-5p displayed a significant differential abundance in purified plasma-derived EVs from patients with PTC and benign disease. However, no difference could be demonstrated in bulk plasma through our cohort of patients. Overall, our work supports the use of a well-defined protocol of plasma-EV miRNAs purification for biomarker discovery, rather than the use of freely-circulating miRNAs in bulk plasma. Our work also demonstrates that standardized pre-analytical and analytical procedures as well as optimized EV-miRNAs detection methods are essential.
Published: 2022

36. Liver ischemia and reperfusion induce periportal expression of necroptosis executor pMLKL wich is associated with early allograft dysfunction after transplantation

Author: UCL - SSS/IREC/CHEX - Pôle de chirgurgie expérimentale et transplantation, UCL - (SLuc) Service de chirurgie et transplantation abdominale, Shi, Shaojun, Bonaccorsi Riani, Eliano, Schurink, Ivo, van den Bosch, Thierry, Doukas, Michael, Lila, Karishma A, Roest, Henk P, Xhema, Daela, Gianello, Pierre, de Jonge, Jeroen, Verstegen, Monique MA, van der Laan, Luc, UCL - SSS/IREC/CHEX - Pôle de chirgurgie expérimentale et transplantation, UCL - (SLuc) Service de chirurgie et transplantation abdominale, Shi, Shaojun, Bonaccorsi Riani, Eliano, Schurink, Ivo, van den Bosch, Thierry, Doukas, Michael, Lila, Karishma A, Roest, Henk P, Xhema, Daela, Gianello, Pierre, de Jonge, Jeroen, Verstegen, Monique MA, and van der Laan, Luc
Abstract: Background: Early allograft dysfunction (EAD) following liver transplantation (LT) remains a major threat to the survival of liver grafts and recipients. In animal models, it is shown that hepatic ischemia-reperfusion injury (IRI) triggers phosphorylation of Mixed Lineage Kinase domain-like protein (pMLKL) inducing necroptotic cell death. However, the clinical implication of pMLKL-mediated cell death in human hepatic IRI remains largely unexplored. In this study, we aimed to investigate the expression of pMLKL in human liver grafts and its association with EAD after LT. Methods: The expression of pMLKL was determined by immunohistochemistry in liver biopsies obtained from both human and rat LT. Human liver biopsies were obtained at the end of preservation (T0) and ~1 hour after reperfusion (T1). The positivity of pMLKL was quantified electronically and compared in rat and human livers and post-LT outcomes. Multiplex immunofluorescence staining was performed to characterize the pMLKL-expressing cells. Results: In the rat LT model, significant pMLKL expression was observed in livers after IRI as compared to livers of sham-operation animals. Similarly, the pMLKL score was highest after IRI in human liver grafts (in T1 biopsies). Both in rats and humans, the pMLKL expression is mostly observed in the portal triads. In grafts who developed EAD after LT (n=24), the pMLKL score at T1 was significantly higher as compared to non-EAD grafts (n=40). ROC curve revealed a high predictive value of pMLKL score at T1 (AUC 0.70) and the ratio of pMLKL score at T1 and T0 (pMLKL-index, AUC 0.82) for EAD. Liver grafts with a high pMLKL index (>1.64) had significantly higher levels of serum ALT, AST, and LDH 24 hours after LT compared to grafts with a low pMLKL index. Multivariate logistical regression analysis identified the pMLKL-index (Odds ratio=1.3, 95% CI 1.1-1.7) as a predictor of EAD development. Immunohistochemistry on serial sections and multiplex staining identified the perip
Published: 2022

37. New approaches to the diagnosis of rejection and prediction of tolerance in liver transplantation

Author: UCL - SSS/IREC/CHEX - Pôle de chirgurgie expérimentale et transplantation, UCL - (SLuc) Service de chirurgie et transplantation abdominale, Taner, Timucin, Bruner, Julia, Emamaullee, Juliet, Bonaccorsi Riani, Eliano, Zarrinpar, Ali, UCL - SSS/IREC/CHEX - Pôle de chirgurgie expérimentale et transplantation, UCL - (SLuc) Service de chirurgie et transplantation abdominale, Taner, Timucin, Bruner, Julia, Emamaullee, Juliet, Bonaccorsi Riani, Eliano, and Zarrinpar, Ali
Abstract: Immunosuppression after liver transplantation is essential for preventing allograft rejection. However, long-term drug toxicity and associated complications necessitate investigation of immunosuppression minimization and withdrawal protocols. Development of such protocols is hindered by reliance on current paradigms for monitoring allograft function and rejection status. The current standard of care for diagnosis of rejection is histopathologic assessment and grading of liver biopsies in accordance with the Banff Rejection Activity Index. However, this method is limited by cost, sampling variability, and interobserver variation. Moreover, the invasive nature of biopsy increases the risk of patient complications. Incorporating noninvasive techniques may supplement existing methods through improved understanding of rejection causes, hepatic spatial architecture, and the role of idiopathic fibroinflammatory regions. These techniques may also aid in quantification and help integrate emerging -omics analyses with current assessments. Alternatively, emerging noninvasive methods show potential to detect and distinguish between different types of rejection while minimizing risk of adverse advents. Although biomarkers have yet to replace biopsy, preliminary studies suggest that several classes of analytes may be used to detect rejection with greater sensitivity and in earlier stages than traditional methods, possibly when coupled with artificial intelligence. Here, we provide an overview of the latest efforts in optimizing the diagnosis of rejection in liver transplantation.
Published: 2022

38. Editorial: Metastatic prostate cancer: a wealth of data to put into practice.

Author: UCL - SSS/IREC/CHEX - Pôle de chirgurgie expérimentale et transplantation, UCL - (SLuc) Service de chirurgie et transplantation abdominale, UCL - (SLuc) Service d'urologie, Tombal, Bertrand, Beltran, Himisha, UCL - SSS/IREC/CHEX - Pôle de chirgurgie expérimentale et transplantation, UCL - (SLuc) Service de chirurgie et transplantation abdominale, UCL - (SLuc) Service d'urologie, Tombal, Bertrand, and Beltran, Himisha
Published: 2022

39. Robotic coronary revascularization in Europe, state of art and future of EACTS-endorsed Robotic Cardiothoracic Surgery Taskforce.

Author: UCL - SSS/IREC/MONT - Pôle Mont Godinne, UCL - (MGD) Service de chirurgie cardio-vasculaire et thoracique, Pettinari, Matto, Gianoli, Monica, Palmen, Meindert, Cerny, Stepan, Onan, Burak, Singh, Sandeep, Segers, Patrique, Bolcal, Cengiz, Alhan, Cem, Navarra, Emiliano, De Praetere, Herbert, Vojacek, Jan, Cebotaru, Theodor, Modi, Paul, Doguet, Fabien, Franke, Ulrich, Ouda, Ahmed, Melly, Ludovic, Malapert, Ghislain, Labrousse, Louis, Agnino, Alfonso, Philipsen, Tine, Jansens, Jean-Luc, Folliguet, Thierry, Pereda, Daniel, Musumeci, Francesco, Suwalski, Piotr, Cathenis, Koen, Van Praet, Frank, Bonatti, Johannes, Oosterlinck, Wouter, European Robotic CardioThoracic Surgeons (ERCTS), UCL - SSS/IREC/MONT - Pôle Mont Godinne, UCL - (MGD) Service de chirurgie cardio-vasculaire et thoracique, Pettinari, Matto, Gianoli, Monica, Palmen, Meindert, Cerny, Stepan, Onan, Burak, Singh, Sandeep, Segers, Patrique, Bolcal, Cengiz, Alhan, Cem, Navarra, Emiliano, De Praetere, Herbert, Vojacek, Jan, Cebotaru, Theodor, Modi, Paul, Doguet, Fabien, Franke, Ulrich, Ouda, Ahmed, Melly, Ludovic, Malapert, Ghislain, Labrousse, Louis, Agnino, Alfonso, Philipsen, Tine, Jansens, Jean-Luc, Folliguet, Thierry, Pereda, Daniel, Musumeci, Francesco, Suwalski, Piotr, Cathenis, Koen, Van Praet, Frank, Bonatti, Johannes, Oosterlinck, Wouter, and European Robotic CardioThoracic Surgeons (ERCTS)
Published: 2022

40. Fucoidan hydrogels significantly alleviate oxidative stress and enhance the endocrine function of encapsulated beta cells

Author: UCL - SSS/IREC/CHEX - Pôle de chirgurgie expérimentale et transplantation, UCL - (SLuc) Service de chirurgie et transplantation abdominale, Vaithilingam, V, Reys, L.L, Sthijns, M.M.J.P.E, Rademakers, T, De Vries, R, Mohammed S.J, De Bont, D, Jetten, M, De Koning, E, Mourad, Nizar, Gianello, Pierre, Silva, S.S, Reis, R.L, Silva, T.H, Van Apeldoorn,A, 6th Tissue Engeneering and Regenerative Medicine International Society's - Americas Annual Conference, TERMIS 2021, UCL - SSS/IREC/CHEX - Pôle de chirgurgie expérimentale et transplantation, UCL - (SLuc) Service de chirurgie et transplantation abdominale, Vaithilingam, V, Reys, L.L, Sthijns, M.M.J.P.E, Rademakers, T, De Vries, R, Mohammed S.J, De Bont, D, Jetten, M, De Koning, E, Mourad, Nizar, Gianello, Pierre, Silva, S.S, Reis, R.L, Silva, T.H, Van Apeldoorn,A, and 6th Tissue Engeneering and Regenerative Medicine International Society's - Americas Annual Conference, TERMIS 2021
Published: 2022

41. Quel est l’intérêt de comprendre les propriétés du glycocalyx endothélial dans la prise en charge de la COVID-19 ?

Author: UCL - SSS/IREC/MONT - Pôle Mont Godinne, UCL - SSS/IREC/MEDA - Pôle de médecine aiguë, UCL - (MGD) Service d'anesthésiologie, UCL - (MGD) Service de chirurgie cardio-vasculaire et thoracique, Boulanger, Alexandre, Dincq, Anne-Sophie, Rondelet, Benoît, Gourdin, Maximilien, UCL - SSS/IREC/MONT - Pôle Mont Godinne, UCL - SSS/IREC/MEDA - Pôle de médecine aiguë, UCL - (MGD) Service d'anesthésiologie, UCL - (MGD) Service de chirurgie cardio-vasculaire et thoracique, Boulanger, Alexandre, Dincq, Anne-Sophie, Rondelet, Benoît, and Gourdin, Maximilien
Abstract: La COVID-19 est une maladie infectieuse émergente virale causée par la souche de coronavirus SARSCoV-2. Après presque deux années de pandémie, les cliniciens ont beaucoup progressé dans les connaissances des manifestations cliniques de la maladie, notamment en mettant en avant la place du glycocalyx endothélial. Ce dernier est une structure complexe micro-fibrillaire située au pôle apical de la cellule endothéliale, constituant la barrière histologique entre la cellule et la lumière vasculaire et se comportant comme un organe à part entière. L’étude physiopathologique de ce dernier a permis de mettre en évidence l’intérêt de celui-ci dans la compréhension de la COVID-19 et de ses complications. Cette revue a donc pour but d’appréhender cette maladie sous un angle différent et se propose de résumer les données pertinentes pour le clinicien que nous avons apprises depuis deux ans.
Published: 2022

42. Five-year Outcomes of Sleeve Gastrectomy: A Prospective Multicenter study.

Author: UCL - SSS/IREC/CHEX - Pôle de chirgurgie expérimentale et transplantation, UCL - (SLuc) Service de chirurgie et transplantation abdominale, El Moussaoui, Imad, Van Vyve, Etienne, Johanet, Hubert, Dabrowski, André, Piquard, Arnaud, Delaunay, Thierry, Navez, Benoît, Hauters, Philippe, Sirisier, Frank, Loi, Patrizia, Closset, Jean, UCL - SSS/IREC/CHEX - Pôle de chirgurgie expérimentale et transplantation, UCL - (SLuc) Service de chirurgie et transplantation abdominale, El Moussaoui, Imad, Van Vyve, Etienne, Johanet, Hubert, Dabrowski, André, Piquard, Arnaud, Delaunay, Thierry, Navez, Benoît, Hauters, Philippe, Sirisier, Frank, Loi, Patrizia, and Closset, Jean
Abstract: Sleeve gastrectomy (SG) is the most frequently performed bariatric procedure in the world. Our purpose was to evaluate the percentage of excess weight loss (%EWL), resolution of obesity-related comorbidities after SG, and identify predictive factors of weight loss failure. A prospective cohort study of adults who underwent SG during 2014 in 7 Belgian-French centers. Their demographic, preoperative, and postoperative data were prospectively collected and analyzed statistically. Overall, 529 patients underwent SG, with a mean preoperative weight and body mass index BMI) of 118.9 ± 19.9 kg and 42.9 ± 5.5 kg/m, respectively. Body mass index significantly decreased to 32.2 kg/m at 5 years ( < .001). The mean %EWL was 63.6% at 5 years. A significant reduction in dyslipidemia (28.0%-18.2%), obstructive sleep apnea (OSAS) (34.6%-25.1%), and arterial hypertension (HTN) (30.4%-21.5%) was observed after 5 years, but not for diabetes and gastroesophageal reflux disease (GERD). At multivariate analysis, age >50 years old, BMI >50 kg/m, and previous laparoscopic adjustable gastric banding (LAGB) remained independent predictors of weight loss failure. Five years after SG, weight loss was satisfactory; the reduction of comorbidities was significant for dyslipidemia, OSAS, and HTN, but not diabetes and GERD. Age >50 years old, BMI >50 kg/m, and previous LAGB were independent predictors of weight loss failure.
Published: 2022

43. Incidental Prostate Cancer (cT1a-cT1b) Is a Relevant Clinical and Research Entity and Should Be Fully Discussed in the International Prostate Cancer Guidelines.

Author: UCL - SSS/IREC/CHEX - Pôle de chirgurgie expérimentale et transplantation, UCL - (SLuc) Service de chirurgie et transplantation abdominale, Capitanio, Umberto, Autorino, Riccardo, Bandini, Marco, Briganti, Alberto, Cheng, Liang, Cooperberg, Matthew R, Dehò, Federico, Gallina, Andrea, Klotz, Laurence, Lane, Brian, Montironi, Rodolfo, Salonia, Andrea, Stief, Christian, Tombal, Bertrand, Montorsi, Francesco, UCL - SSS/IREC/CHEX - Pôle de chirgurgie expérimentale et transplantation, UCL - (SLuc) Service de chirurgie et transplantation abdominale, Capitanio, Umberto, Autorino, Riccardo, Bandini, Marco, Briganti, Alberto, Cheng, Liang, Cooperberg, Matthew R, Dehò, Federico, Gallina, Andrea, Klotz, Laurence, Lane, Brian, Montironi, Rodolfo, Salonia, Andrea, Stief, Christian, Tombal, Bertrand, and Montorsi, Francesco
Published: 2022

44. Meso-Rex bypass for the management of extrahepatic portal vein obstruction in adults (with video).

Author: UCL - SSS/IREC/CHEX - Pôle de chirgurgie expérimentale et transplantation, UCL - (SLuc) Service de chirurgie et transplantation abdominale, UCL - SSS/IREC - Institut de recherche expérimentale et clinique, UCL - SSS/IONS/NEUR - Clinical Neuroscience, UCL - (SLuc) Service de radiologie, Brichard, Martin, Iesari, Samuele, Lerut, Jan, Reding, Raymond, Goffette, Pierre, Coubeau, Laurent, UCL - SSS/IREC/CHEX - Pôle de chirgurgie expérimentale et transplantation, UCL - (SLuc) Service de chirurgie et transplantation abdominale, UCL - SSS/IREC - Institut de recherche expérimentale et clinique, UCL - SSS/IONS/NEUR - Clinical Neuroscience, UCL - (SLuc) Service de radiologie, Brichard, Martin, Iesari, Samuele, Lerut, Jan, Reding, Raymond, Goffette, Pierre, and Coubeau, Laurent
Abstract: Extrahepatic portal vein obstruction (EHPVO) results in severe portal hypertension (PHT) leading to severely compromised quality of life. Often, pharmacological and endoscopic management are unable to solve this problem. Restoring hepatic portal flow using meso-Rex bypass (MRB) may solve it. This procedure, uncommon in adult patients, is considered the treatment of choice for EHPVO in children. From 1997 to 2018, 8 male and 6 female adults, with a median age of 51 years (range 22-66) underwent MRB procedure for EHPVO at the University Hospitals Saint-Luc in Brussels, Belgium. Symptoms of PHT were life altering in all but one patient and consisted of repetitive gastro-intestinal bleedings, sepsis due to portal biliopathy, and/or severe abdominal discomfort. The surgical technique consisted in interposition of a free venous graft or of a prosthetic graft between the superior mesenteric vein and the Rex recess of the left portal vein. Median operative time was 500 min (range 300-730). Median follow-up duration was 22 months (range 2-169). One patient died due to hemorrhagic shock following percutaneous transluminal intervention for early graft thrombosis. Major morbidity, defined as Clavien-Dindo score ≥ III, was 35.7% (5/14). Shunt patency at last follow-up was 64.3% (9/14): 85.7% (6/7) of pure venous grafts and only 42.9% (3/7) of prosthetic graft. Symptom relief was achieved in 85.7% (12/14) who became asymptomatic after MRB. Adult EHPVO represents a difficult clinical condition that leads to severely compromised quality of life and possible life-threatening complications. In such patients, MRB represents the only and last resort to restore physiological portal vein flow. Although successful in a majority of patients, this procedure is associated with major morbidity and mortality and should be done in tertiary centers experienced with vascular liver surgery to get the best results.
Published: 2022

45. Insights in living-donor liver transplantation associated with two-stage total hepatectomy: First case in neuroendocrine tumor metastases and functional assessment techniques.

Author: UCL - SSS/IREC/CHEX - Pôle de chirgurgie expérimentale et transplantation, UCL - (SLuc) Service de chirurgie et transplantation abdominale, UCL - SSS/IREC/MIRO - Pôle d'imagerie moléculaire, radiothérapie et oncologie, UCL - (SLuc) Service d'anatomie pathologique, UCL - (SLuc) Service de médecine nucléaire, Coubeau, Laurent, Iesari, Samuele, Henry, Paulina, D'Abadie, Philippe, Vanbuggenhout, Aude, Reding, Raymond, UCL - SSS/IREC/CHEX - Pôle de chirgurgie expérimentale et transplantation, UCL - (SLuc) Service de chirurgie et transplantation abdominale, UCL - SSS/IREC/MIRO - Pôle d'imagerie moléculaire, radiothérapie et oncologie, UCL - (SLuc) Service d'anatomie pathologique, UCL - (SLuc) Service de médecine nucléaire, Coubeau, Laurent, Iesari, Samuele, Henry, Paulina, D'Abadie, Philippe, Vanbuggenhout, Aude, and Reding, Raymond
Published: 2022

46. Long-term outcomes of atrioventricular septal defect and single ventricle: A multicenter study.

Author: UCL - SSS/IREC/CARD - Pôle de recherche cardiovasculaire, UCL - (SLuc) Service de chirurgie cardiovasculaire et thoracique, Arrigoni, Sara C, IJsselhof, Rinske, Postmus, Douwe, Vonk, Judith M, François, Katrien, Bové, Thierry, Hazekamp, Mark G, Rijnberg, Friso M, Meyns, Bart, van Puyvelde, Joeri, Poncelet, Alain, de Beco, Geoffroy, van de Woestijne, Pieter C, Bogers, Ad J J C, Schoof, Paul H, Ebels, Tjark, UCL - SSS/IREC/CARD - Pôle de recherche cardiovasculaire, UCL - (SLuc) Service de chirurgie cardiovasculaire et thoracique, Arrigoni, Sara C, IJsselhof, Rinske, Postmus, Douwe, Vonk, Judith M, François, Katrien, Bové, Thierry, Hazekamp, Mark G, Rijnberg, Friso M, Meyns, Bart, van Puyvelde, Joeri, Poncelet, Alain, de Beco, Geoffroy, van de Woestijne, Pieter C, Bogers, Ad J J C, Schoof, Paul H, and Ebels, Tjark
Abstract: The study objective was to analyze survival and incidence of Fontan completion of patients with single-ventricle and concomitant unbalanced atrioventricular septal defect. Data from 4 Dutch and 3 Belgian institutional databases were retrospectively collected. A total of 151 patients with single-ventricle atrioventricular septal defect were selected; 36 patients underwent an atrioventricular valve procedure (valve surgery group). End points were survival, incidence of Fontan completion, and freedom from atrioventricular valve reoperation. Median follow-up was 13.4 years. Cumulative survival was 71.2%, 70%, and 68.5% at 10, 15, and 20 years, respectively. An atrioventricular valve procedure was not a risk factor for mortality. Patients with moderate-severe or severe atrioventricular valve regurgitation at echocardiographic follow-up had a significantly worse 15-year survival (58.3%) compared with patients with no or mild regurgitation (89.2%) and patients with moderate regurgitation (88.6%) (P = .033). Cumulative incidence of Fontan completion was 56.5%, 71%, and 77.6% at 5, 10, and 15 years, respectively. An atrioventricular valve procedure was not associated with the incidence of Fontan completion. In the valve surgery group, freedom from atrioventricular valve reoperation was 85.7% at 1 year and 52.6% at 5 years. The long-term survival and incidence of Fontan completion in our study were better than previously described for patients with single-ventricle atrioventricular septal defect. A concomitant atrioventricular valve procedure did not increase the mortality rate or decrease the incidence of Fontan completion, whereas patients with moderate-severe or severe valve regurgitation at follow-up had a worse survival. Therefore, in patients with single-ventricle atrioventricular septal defect when atrioventricular valve regurgitation exceeds a moderate degree, the atrioventricular valve should be repaired.
Published: 2022

47. Rapid Decline in Vaccine Induced anti-SARS-CoV-2 Antibody Titers 3 Months After Kidney Transplantation: A Case Series From Belgium.

Author: UCL - SSS/IREC/NEFR - Pôle de Néphrologie, UCL - (SLuc) Service de néphrologie, UCL - SSS/IREC - Institut de recherche expérimentale et clinique, UCL - (SLuc) Service de microbiologie, UCL - SSS/IREC/LTAP - Louvain Centre for Toxicology and Applied Pharmacology, UCL - (SLuc) Service de médecine interne générale, UCL - SSS/IREC/CHEX - Pôle de chirgurgie expérimentale et transplantation, UCL - (SLuc) Service de chirurgie et transplantation abdominale, UCL - SSS/IREC/MBLG - Pôle de Microbiologie médicale, Fernandes, Guillaume, Devresse, Arnaud, Scohy, Anaïs, Yombi, Jean Cyr, Belkhir, Leïla, De Greef, Julien, De Meyer, Martine, Mourad, Michel, Darius, Tom, Buemi, Antoine, Kabamba Mukadi, Benoît, Goffin, Eric, Kanaan, Nada, UCL - SSS/IREC/NEFR - Pôle de Néphrologie, UCL - (SLuc) Service de néphrologie, UCL - SSS/IREC - Institut de recherche expérimentale et clinique, UCL - (SLuc) Service de microbiologie, UCL - SSS/IREC/LTAP - Louvain Centre for Toxicology and Applied Pharmacology, UCL - (SLuc) Service de médecine interne générale, UCL - SSS/IREC/CHEX - Pôle de chirgurgie expérimentale et transplantation, UCL - (SLuc) Service de chirurgie et transplantation abdominale, UCL - SSS/IREC/MBLG - Pôle de Microbiologie médicale, Fernandes, Guillaume, Devresse, Arnaud, Scohy, Anaïs, Yombi, Jean Cyr, Belkhir, Leïla, De Greef, Julien, De Meyer, Martine, Mourad, Michel, Darius, Tom, Buemi, Antoine, Kabamba Mukadi, Benoît, Goffin, Eric, and Kanaan, Nada
Published: 2022

48. Monoclonal Antibody Therapy for SARS-CoV-2 Infection in Kidney Transplant Recipients: A Case Series From Belgium.

Author: UCL - SSS/IREC/NEFR - Pôle de Néphrologie, UCL - (SLuc) Service de néphrologie, UCL - SSS/IREC - Institut de recherche expérimentale et clinique, UCL - (SLuc) Service de microbiologie, UCL - (SLuc) Service de médecine interne générale, UCL - SSS/IREC/LTAP - Louvain Centre for Toxicology and Applied Pharmacology, UCL - SSS/IREC/CHEX - Pôle de chirgurgie expérimentale et transplantation, UCL - (SLuc) Service de chirurgie et transplantation abdominale, UCL - SSS/IREC/MBLG - Pôle de Microbiologie médicale, Fernandes, Guillaume, Devresse, Arnaud, Scohy, Anaïs, Yombi, Jean Cyr, Belkhir, Leïla, De Greef, Julien, Darius, Tom, Buemi, Antoine, Kabamba Mukadi, Benoît, Goffin, Eric, Kanaan, Nada, UCL - SSS/IREC/NEFR - Pôle de Néphrologie, UCL - (SLuc) Service de néphrologie, UCL - SSS/IREC - Institut de recherche expérimentale et clinique, UCL - (SLuc) Service de microbiologie, UCL - (SLuc) Service de médecine interne générale, UCL - SSS/IREC/LTAP - Louvain Centre for Toxicology and Applied Pharmacology, UCL - SSS/IREC/CHEX - Pôle de chirgurgie expérimentale et transplantation, UCL - (SLuc) Service de chirurgie et transplantation abdominale, UCL - SSS/IREC/MBLG - Pôle de Microbiologie médicale, Fernandes, Guillaume, Devresse, Arnaud, Scohy, Anaïs, Yombi, Jean Cyr, Belkhir, Leïla, De Greef, Julien, Darius, Tom, Buemi, Antoine, Kabamba Mukadi, Benoît, Goffin, Eric, and Kanaan, Nada
Published: 2022

49. European Liver Transplant Registry: donor and transplant surgery aspects of 16,641 liver transplantations in children.

Author: UCL - SSS/IREC/CHEX - Pôle de chirgurgie expérimentale et transplantation, UCL - (SLuc) Service de chirurgie et transplantation abdominale, de Ville de Goyet, Jean, Baumann, Ulrich, Karam, Vincent, Adam, René, Nadalin, Silvio, Heaton, Nigel, Reding, Raymond, Branchereau, Sophie, Mirza, Darius, Klempnauer, Jürgen L, Fischer, Lutz, Kalicinski, Piotr, Colledan, Michele, Lopez Santamaria, Manuel, de Kleine, Ruben H, Chardot, Christophe, Yilmaz, Sezai, Kilic, Murat, Boillot, Olivier, di Francesco, Fabrizio, Polak, Wojciech G, Verkade, Henkjan J, European Liver, Intestine Transplant Association (ELITA), UCL - SSS/IREC/CHEX - Pôle de chirgurgie expérimentale et transplantation, UCL - (SLuc) Service de chirurgie et transplantation abdominale, de Ville de Goyet, Jean, Baumann, Ulrich, Karam, Vincent, Adam, René, Nadalin, Silvio, Heaton, Nigel, Reding, Raymond, Branchereau, Sophie, Mirza, Darius, Klempnauer, Jürgen L, Fischer, Lutz, Kalicinski, Piotr, Colledan, Michele, Lopez Santamaria, Manuel, de Kleine, Ruben H, Chardot, Christophe, Yilmaz, Sezai, Kilic, Murat, Boillot, Olivier, di Francesco, Fabrizio, Polak, Wojciech G, Verkade, Henkjan J, and European Liver, Intestine Transplant Association (ELITA)
Abstract: The European Liver Transplant Registry (ELTR) has collected data on liver transplant procedures performed in Europe since 1968. Over a 50 years period (1968 - 2017), clinical and laboratory data were collected from 133 transplant centers and analyzed retrospectively (16,641 liver transplants in 14,515 children). Data were analyzed according to 3 successive periods (A: before 2000, B: 2000 to 2009, and C: since 2010), studying donor and graft characteristics, and graft outcome. The use of living donors steadily increased from A to C [A: n=296 (7%), B: n=1131 (23%) and C: n=1985 (39%); P=0.0001]. Overall, the 5-year graft survival rate has improved from 65% in group A to 75% in group B (p<0.0001), and to 79% in group C (B vs C, p<0.0001). Graft half-life was 31 years, overall; it was 41 years for children who survived the first year after transplant. The late annual graft loss rate in teen-agers is higher than children aged < 12 years, and similar to that of young adults. No evidence for accelerated graft loss after age 18 year was found. Pediatric liver transplantation has reached a high efficacy as a cure, or treatment, for severe liver disease in infants and children. Grafts that survived the first year had a half-life time similar to standard human half-life. Transplantation before or after puberty may be the pivot-point for lower long-term outcome in children. Further studies are necessary to re-visit some old concepts regarding transplant benefit (survival time) for small children, the role of recipient pathophysiology versus graft aging, and risk at transition to adult age.
Published: 2022

50. A Rare Case of Late LAD Reimplantation after Arterial Switch Operation

Author: UCL - SSS/IREC/CARD - Pôle de recherche cardiovasculaire, UCL - (SLuc) Service de chirurgie cardiovasculaire et thoracique, UCL - SSS/IREC/PEDI - Pôle de Pédiatrie, UCL - (SLuc) Service de cardiologie pédiatrique, Kabulo Kolela, Yannick, Klepper, Maureen, de Beco, Geoffroy, Sluysmans, Thierry, Poncelet, Alain, UCL - SSS/IREC/CARD - Pôle de recherche cardiovasculaire, UCL - (SLuc) Service de chirurgie cardiovasculaire et thoracique, UCL - SSS/IREC/PEDI - Pôle de Pédiatrie, UCL - (SLuc) Service de cardiologie pédiatrique, Kabulo Kolela, Yannick, Klepper, Maureen, de Beco, Geoffroy, Sluysmans, Thierry, and Poncelet, Alain
Abstract: Arterial switch operation (ASO) is a complex neonatal operation in which transfer of the coronary arteries origins is the key to success. Coronary events after a successful ASO are not uncommon. We describe a rare case of a child who underwent an ASO in the neonatal period with one coronary (LAD) described as atretic left in place. At age seven, he developed myocardial ischemia due to retrograde flow with a steal phenomenon from the LAD into the pulmonary artery. The patient underwent a late LAD reimplantation. This case underscores that even very small ostia should be translocated at the time of ASO.
Published: 2022

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