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1. Clinical impact of transient lymphopenia

Author

Petramala, Luigi, Milito, Cinzia, Sarlo, Francesca, Servello, Adriana, Circosta, Francesco, Marino, Luca, Sardella, Germano, Trapani, Piero, D’aguanno, Giulio, Cimo’, Antonino, Galardo, Gioacchino, and Letizia, Claudio

Published
2024
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3. Relevance of microbiological cultures of cord blood and placental swabs in the rapid diagnosis of preterm newborn infection due to Listeria monocytogenes: A case report

Author

Francesco D'Aleo, Attilio Tuscano, Tarcisio Servello, Marcello Tripodi, Carmela Abramo, Roberta Bonanno, Ferdinando Antonio Gulino, Sara Occhipinti, Giosuè Giordano Incognito, and Luigi Principe

Subjects
Listeria monocytogenes, Neonatal infections, Case report, Placenta swabs, Cord blood, Surgery, RD1-811, Gynecology and obstetrics, RG1-991
Abstract

Listeria monocytogenes (Lm) is a Gram-positive bacterium causing listeriosis, a rare but severe foodborne infection, particularly impactful during pregnancy. Maternal-fetal transmission can lead to adverse fetal outcomes, yet symptoms in mothers may be nonspecific, delaying intervention. Despite the severity, the mechanisms of vertical transmission remain unclear. This report describes a case of rapid Lm diagnosis in a preterm newborn using cord blood and placental swabs. A 31-week pregnant woman presented with abdominal pain, diarrhea, and reduced fetal movements after consuming raw sushi. Laboratory findings indicated infection, and she vaginally delivered a live infant with placental and fetal abscesses. Cultures confirmed Lm, with swift diagnosis aided by molecular syndromic testing. The neonate received appropriate antibiotics and was asymptomatic by the end of treatment. This case underscores the need for the rapid diagnosis of maternal-fetal listeriosis, as it poses significant risks during pregnancy, including preterm birth and neonatal complications. Current diagnostic methods often delay treatment. This report emphasizes the use of innovative molecular techniques for early diagnosis, which is crucial in managing neonatal infections, especially in preterm newborns.

Published
2024
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5. Mapping Gilles de la Tourette syndrome through the distress and relief associated with tic-related behaviors: an fMRI study

Author

Laura Zapparoli, Francantonio Devoto, Marika Mariano, Silvia Seghezzi, Domenico Servello, Mauro Porta, and Eraldo Paulesu

Subjects
Neurosciences. Biological psychiatry. Neuropsychiatry, RC321-571
Abstract

Abstract Personal distress associated with tic urges or inhibition and relief associated with tic production are defining features of the personal experience in Gilles de la Tourette syndrome (GTS). These affective phenomena have not been studied using fMRI, hindering our understanding of GTS pathophysiology and possible treatments. Here, we present a novel cross-sectional fMRI study designed to map tic-related phenomenology using distress and relief as predicting variables. We adopted a mental imagery approach and dissected the brain activity associated with different phases of tic behaviors, premonitory urges, and the ensuing tic execution or inhibition: these were compared with the mental simulation of “relaxed situations” and pre-determined stereotyped motor behaviors. We then explored whether the ensuing brain patterns correlated with the distress or relief perceived for the different phases of the tasks. Patients experienced a higher level of distress during the imagery of tic-triggering scenarios and no relief during tic inhibition. On the other hand, patients experienced significant relief during tic imagery. Distress during tic-triggering scenarios and relief during tic imagery were significantly correlated. The distress perceived during urges correlated with increased activation in cortical sensorimotor areas, suggesting a motor alarm. Conversely, relief during tic execution was positively associated with the activity of a subcortical network. The activity of the putamen was associated with both distress during urges and relief during tic execution. These findings highlight the importance of assessing the affective component of tic-related phenomenology. Subcortical structures may be causally involved in the affective component of tic pathophysiology, with the putamen playing a central role in both tic urge and generation. We believe that our results can be readily translated into clinical practice for the development of personalized treatment plans tailored to each patient’s unique needs.

Published
2024
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7. Relevance of obesity-related organ damage and metabolic syndrome classification in cardiovascular and renal risk stratification in patients with essential hypertension

Author

Luigi Petramala, Antonietta Gigante, Francesca Sarlo, Adriana Servello, Francesco Circosta, Luca Marino, Antonello Ciccarelli, Giuseppe Cavallaro, and Claudio Letizia

Subjects
hypertension, metabolic syndrome, cardiovascular risk, organ target damage, chronic kidney disease, obesity, Diseases of the circulatory (Cardiovascular) system, RC666-701
Abstract

IntroductionHypertension is a relevant cardiovascular comorbidity. Adipose tissue represents a metabolically active tissue involved in the regulation of blood pressure and metabolic alterations. In recent decades, several classifications for the metabolic syndrome (MS) have been proposed. Recently, a new syndrome called the “Cardiovascular-kidney-metabolic” (CKM) syndrome was identified, to determine patients at high cardiovascular and metabolic risk. The aim of the study was to compare different classifications in a large population of hypertensive patients.Materials and methodsBetween September 2022 and August 2023, we consecutively enrolled 772 hypertensive patients (407 men; 365 women; mean age 52.2 ± 15.1 years), evaluating anthropometric, biochemical, and instrumental parameters (transthoracic echocardiogram, carotid echo-Doppler, 24-h ambulatory blood pressure monitoring, fundus oculi).ResultsUsing different classifications we found MS prevalence: Adult Treatment Panel III (ATP-III) 28.8%, International Diabetes Federation (IDF) 31.5%, CKM 40.7%. CKM Classes 3 and 4 showed higher body mass index and waist circumference compared with other groups. Compared with ATP-III and IDF, CKM Class 4 showed higher 24-h systolic blood pressure, lower percentage of controlled hypertension, increased interventricular septum and posterior wall, reduced ejection fraction, and greater prevalence of hypertensive arterial retinal damage.DiscussionVisceral obesity and MS are frequent conditions with healthy impact, becoming an important trigger for the development of cardiovascular and metabolic complications. The different MS classifications allow the early identification of patients at high risk of cardiometabolic complications. The new CKM syndrome proves useful to identify individuals at high risk for CKM morbidity and mortality.

Published
2024
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8. Clinical Evaluation of Adrenal Incidentaloma: The Experience of a Referral Center

Author

Luigi Petramala, Francesco Circosta, Luca Marino, Edoardo Palombi, Maria Ludovica Costanzo, Adriana Servello, Gioacchino Galardo, and Claudio Letizia

Subjects
adrenal incidentaloma, dexamethasone test, hypertension, metabolic disorders, Biology (General), QH301-705.5
Abstract

The number of adrenal incidentaloma (AI) cases has increased in the last few years due to the widespread use of imaging diagnostics. Management requires evaluation of the malignant nature and hormonal activity. The aim of the present study is to assess possible clinical abnormalities in 132 AI patients both at baseline and during follow-up (mean 48.6 ± 12.5 months). In all patients, demographic, anthropometric data, biochemical, metabolic and hormonal data, and 24-h ambulatory blood pressure monitoring were assessed. Mild autonomous cortisol secretions (MACS) were diagnosed in patients without signs and symptoms of overt Cushing’s syndrome and post dexamethasone (DXM) plasma cortisol concentration > 50 nmol/L (>1.8 μg/dL). Patients with overnight DXM-1 mg test positive showed higher values of diastolic blood pressure, glycemia and uric acid levels compared to patients with negative DXM test at baseline. During follow-up, the potential development of MACS in patients with nonfunctional AI showed a prevalence of 29%, though the cardiovascular and metabolic alterations were less pronounced compared to those diagnosed with MACS at baseline. Therefore, follow-ups with AI patients are useful for observing changes in clinical features.

Published
2024
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9. MER and increased operative time are not risk factors for the formation of pneumocephalus during DBS

Author

Guglielmo Iess, Giulio Bonomo, Vincenzo Levi, Domenico Aquino, Edvin Zekaj, Federica Mezza, and Domenico Servello

Subjects
Medicine, Science
Abstract

Abstract Although only recently directional leads have proven their potential to compensate for sub-optimally placed electrodes, optimal lead positioning remains the most critical factor in determining Deep Brain Stimulation (DBS) outcome. Pneumocephalus is a recognized source of error, but the factors that contribute to its formation are still a matter of debate. Among these, operative time is one of the most controversial. Because cases of DBS performed with Microelectrode Recordings (MER) are affected by an increase in surgical length, it is useful to analyze whether MER places patients at risk for increased intracranial air entry. Data of 94 patients from two different institutes who underwent DBS for different neurologic and psychiatric conditions were analyzed for the presence of postoperative pneumocephalus. Operative time and use of MER, as well as other potential risk factors for pneumocephalus (age, awake vs. asleep surgery, number of MER passages, burr hole size, target and unilateral vs. bilateral implants) were examined. Mann-Whitney U and Kruskal-Wallis tests were utilized to compare intracranial air distributions across groups of categorical variables. Partial correlations were used to assess the association between time and volume. A generalized linear model was created to predict the effects of time and MER on the volume of intracranial air, controlling for other potential risk factors identified: age, number of MER passages, awake vs. asleep surgery, burr hole size, target, unilateral vs. bilateral surgery. Significantly different distributions of air volume were noted between different targets, unilateral vs. bilateral implants, and number of MER trajectories. Patients undergoing DBS with MER did not present a significant increase in pneumocephalus compared to patients operated without (p = 0.067). No significant correlation was found between pneumocephalus and time. Using multivariate analysis, unilateral implants exhibited lower volumes of pneumocephalus (p = 0.002). Two specific targets exhibited significantly different volumes of pneumocephalus: the bed nucleus of the stria terminalis with lower volumes (p < 0.001) and the posterior hypothalamus with higher volumes (p = 0.011). MER, time, and other parameters analyzed failed to reach statistical significance. Operative time and use of intraoperative MER are not significant predictors of pneumocephalus during DBS. Air entry is greater for bilateral surgeries and may be also influenced by the specific stimulated target.

Published
2023
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13. Image-based analysis and long-term clinical outcomes of deep brain stimulation for Tourette syndrome: a multisite study

Author

Johnson, Kara A, Fletcher, P Thomas, Servello, Domenico, Bona, Alberto, Porta, Mauro, Ostrem, Jill L, Bardinet, Eric, Welter, Marie-Laure, Lozano, Andres M, Baldermann, Juan Carlos, Kuhn, Jens, Huys, Daniel, Foltynie, Thomas, Hariz, Marwan, Joyce, Eileen M, Zrinzo, Ludvic, Kefalopoulou, Zinovia, Zhang, Jian-guo, Meng, Fan-gang, Zhang, ChenCheng, Ling, Zhipei, Xu, Xin, Yu, Xinguang, Smeets, Anouk YJM, Ackermans, Linda, Visser-Vandewalle, Veerle, Mogilner, Alon Y, Pourfar, Michael H, Almeida, Leonardo, Gunduz, Aysegul, Hu, Wei, Foote, Kelly D, Okun, Michael S, and Butson, Christopher R

Subjects
Biomedical and Clinical Sciences, Neurosciences, Clinical Sciences, Rehabilitation, Assistive Technology, Brain Disorders, Clinical Research, Tourette Syndrome, Neurodegenerative, Bioengineering, Adolescent, Adult, Atlases as Topic, Cohort Studies, Compulsive Behavior, Deep Brain Stimulation, Female, Globus Pallidus, Humans, Internal Capsule, Intralaminar Thalamic Nuclei, Magnetic Resonance Imaging, Male, Middle Aged, Nucleus Accumbens, Obsessive Behavior, Retrospective Studies, Severity of Illness Index, Thalamus, Tomography, X-Ray Computed, Treatment Outcome, Young Adult, globus pallidus, neuromodulation, obsessive-compulsive behavior, thalamus, tics, Medical and Health Sciences, Psychology and Cognitive Sciences, Neurology & Neurosurgery, Clinical sciences
Abstract

BackgroundDeep brain stimulation (DBS) can be an effective therapy for tics and comorbidities in select cases of severe, treatment-refractory Tourette syndrome (TS). Clinical responses remain variable across patients, which may be attributed to differences in the location of the neuroanatomical regions being stimulated. We evaluated active contact locations and regions of stimulation across a large cohort of patients with TS in an effort to guide future targeting.MethodsWe collected retrospective clinical data and imaging from 13 international sites on 123 patients. We assessed the effects of DBS over time in 110 patients who were implanted in the centromedial (CM) thalamus (n=51), globus pallidus internus (GPi) (n=47), nucleus accumbens/anterior limb of the internal capsule (n=4) or a combination of targets (n=8). Contact locations (n=70 patients) and volumes of tissue activated (n=63 patients) were coregistered to create probabilistic stimulation atlases.ResultsTics and obsessive-compulsive behaviour (OCB) significantly improved over time (p0.05). The median time was 13 months to reach a 40% improvement in tics, and there were no significant differences across targets (p=0.84), presence of OCB (p=0.09) or age at implantation (p=0.08). Active contacts were generally clustered near the target nuclei, with some variability that may reflect differences in targeting protocols, lead models and contact configurations. There were regions within and surrounding GPi and CM thalamus that improved tics for some patients but were ineffective for others. Regions within, superior or medial to GPi were associated with a greater improvement in OCB than regions inferior to GPi.ConclusionThe results collectively indicate that DBS may improve tics and OCB, the effects may develop over several months, and stimulation locations relative to structural anatomy alone may not predict response. This study was the first to visualise and evaluate the regions of stimulation across a large cohort of patients with TS to generate new hypotheses about potential targets for improving tics and comorbidities.

Published
2019

16. Balanced Activity between Kv3 and Nav Channels Determines Fast-Spiking in Mammalian Central Neurons

Author

Gu, Yuanzheng, Servello, Dustin, Han, Zhi, Lalchandani, Rupa R, Ding, Jun B, Huang, Kun, and Gu, Chen

Subjects
Biomedical and Clinical Sciences, Neurosciences, 1.1 Normal biological development and functioning, Underpinning research, Biophysics, Molecular Neuroscience, Neuroscience
Abstract

Fast-spiking (FS) neurons can fire action potentials (APs) up to 1,000 Hz and play key roles in vital functions such as sound location, motor coordination, and cognition. Here we report that the concerted actions of Kv3 voltage-gated K+ (Kv) and Na+ (Nav) channels are sufficient and necessary for inducing and maintaining FS. Voltage-clamp analysis revealed a robust correlation between the Kv3/Nav current ratio and FS. Expressing Kv3 channels alone could convert ∼30%-60% slow-spiking (SS) neurons to FS in culture. In contrast, co-expression of either Nav1.2 or Nav1.6 together with Kv3.1 or Kv3.3, but not alone or with Kv1.2, converted SS to FS with 100% efficiency. Furthermore, RNA-sequencing-based genome-wide analysis revealed that the Kv3/Nav ratio and Kv3 expression levels strongly correlated with the maximal AP frequencies. Therefore, FS is established by the properly balanced activities of Kv3 and Nav channels and could be further fine-tuned by channel biophysical features and localization patterns.

Published
2018

19. Relevance of obesity-related organ damage and metabolic syndrome classification in cardiovascular and renal risk stratification in patients with essential hypertension

Author

Petramala, Luigi, primary, Gigante, Antonietta, additional, Sarlo, Francesca, additional, Servello, Adriana, additional, Circosta, Francesco, additional, Marino, Luca, additional, Ciccarelli, Antonello, additional, Cavallaro, Giuseppe, additional, and Letizia, Claudio, additional

Published
2024
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21. Neuronal temperature perception induces specific defenses that enable C. elegans to cope with the enhanced reactivity of hydrogen peroxide at high temperature

Author

Francesco A Servello, Rute Fernandes, Matthias Eder, Nathan Harris, Olivier MF Martin, Natasha Oswal, Anders Lindberg, Nohelly Derosiers, Piali Sengupta, Nicholas Stroustrup, and Javier Apfeld

Subjects
C. elegans, temperature, insulin signaling, sensory perception, Enterococcus faecium, hydrogen peroxide, Medicine, Science, Biology (General), QH301-705.5
Abstract

Hydrogen peroxide is the most common reactive chemical that organisms face on the microbial battlefield. The rate with which hydrogen peroxide damages biomolecules required for life increases with temperature, yet little is known about how organisms cope with this temperature-dependent threat. Here, we show that Caenorhabditis elegans nematodes use temperature information perceived by sensory neurons to cope with the temperature-dependent threat of hydrogen peroxide produced by the pathogenic bacterium Enterococcus faecium. These nematodes preemptively induce the expression of specific hydrogen peroxide defenses in response to perception of high temperature by a pair of sensory neurons. These neurons communicate temperature information to target tissues expressing those defenses via an insulin/IGF1 hormone. This is the first example of a multicellular organism inducing their defenses to a chemical when they sense an inherent enhancer of the reactivity of that chemical.

Published
2022
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22. CONSIDERAÇÕES ACERCA DA PESQUISA SOCIOLÓGICA: o materialismo histórico hoje e suas implicações no debate científico contemporâneo

Author

José Carlos SERVELLO JÚNIOR

Subjects
metodologia, materialismo histórico, ciências sociais, Anthropology, GN1-890, Political science, Social Sciences
Abstract

Este artigo procura refletir sobre a atualidade do materialismo histórico, debatendo com outras correntes metodológicas que acreditam que ele não mais é viável nos dias de hoje.

Published
2021

23. Mapping Gilles de la Tourette syndrome through the distress and relief associated with tic-related behaviors: an fMRI study

Author

Zapparoli, L, Devoto, F, Mariano, M, Seghezzi, S, Servello, D, Porta, M, Paulesu, E, Zapparoli, L, Devoto, F, Mariano, M, Seghezzi, S, Servello, D, Porta, M, and Paulesu, E

Abstract

Personal distress associated with tic urges or inhibition and relief associated with tic production are defining features of the personal experience in Gilles de la Tourette syndrome (GTS). These affective phenomena have not been studied using fMRI, hindering our understanding of GTS pathophysiology and possible treatments. Here, we present a novel cross-sectional fMRI study designed to map tic-related phenomenology using distress and relief as predicting variables. We adopted a mental imagery approach and dissected the brain activity associated with different phases of tic behaviors, premonitory urges, and the ensuing tic execution or inhibition: these were compared with the mental simulation of “relaxed situations” and pre-determined stereotyped motor behaviors. We then explored whether the ensuing brain patterns correlated with the distress or relief perceived for the different phases of the tasks. Patients experienced a higher level of distress during the imagery of tic-triggering scenarios and no relief during tic inhibition. On the other hand, patients experienced significant relief during tic imagery. Distress during tic-triggering scenarios and relief during tic imagery were significantly correlated. The distress perceived during urges correlated with increased activation in cortical sensorimotor areas, suggesting a motor alarm. Conversely, relief during tic execution was positively associated with the activity of a subcortical network. The activity of the putamen was associated with both distress during urges and relief during tic execution. These findings highlight the importance of assessing the affective component of tic-related phenomenology. Subcortical structures may be causally involved in the affective component of tic pathophysiology, with the putamen playing a central role in both tic urge and generation. We believe that our results can be readily translated into clinical practice for the development of personalized treatment plans tailored to eac

Published
2024

24. Prevalence of children born small for gestational age with short stature who qualify for growth hormone treatment

Author

Gianluca Tamaro, Mariagrazia Pizzul, Giuliana Gaeta, Raffaella Servello, Marina Trevisan, Patricia Böhm, Paola Manera Ada Materassi, Anna Macaluso, Denis Valentini, Maria Chiara Pellegrin, Egidio Barbi, and Gianluca Tornese

Subjects
Small-for-gestational age, Growth hormone, Short stature, Catch-up growth, Italy, Pediatrics, RJ1-570
Abstract

Abstract Background Recombinant human growth hormone (rhGH) is approved in Europe as a treatment for short children born small for gestational age (SGA) since 2003. However, no study evaluated the prevalence of SGA children with short stature who qualify for rhGH in Europe so far. This study aimed to investigate in an Italian population the prevalence of children born SGA, of short stature in children born SGA, and of SGA children who qualify for rhGH treatment at 4 years of age. Methods We conducted a population-based study on primary care pediatricians’ databases in Trieste, Italy. Data was collected on 3769 children born between 2004 and 2014. SGA was defined as birth weight and/or birth length ≤ − 2 SDS. Data on height and weight were registered at the closest well-being visit to 1, 2, 3, 4 years of age. Short stature was defined as height ≤ − 2 SDS. Short children born SGA who qualify for rhGH treatment were identified according to Note AIFA #39 criteria (age ≥ 4 years; height ≤ − 2.5 SDS; growth velocity

Published
2021
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25. Clinical Evaluation of Adrenal Incidentaloma: The Experience of a Referral Center.

Author

Petramala, Luigi, Circosta, Francesco, Marino, Luca, Palombi, Edoardo, Costanzo, Maria Ludovica, Servello, Adriana, Galardo, Gioacchino, and Letizia, Claudio

Subjects
AMBULATORY blood pressure monitoring, CUSHING'S syndrome, DIASTOLIC blood pressure, URIC acid, SYMPTOMS
Abstract

The number of adrenal incidentaloma (AI) cases has increased in the last few years due to the widespread use of imaging diagnostics. Management requires evaluation of the malignant nature and hormonal activity. The aim of the present study is to assess possible clinical abnormalities in 132 AI patients both at baseline and during follow-up (mean 48.6 ± 12.5 months). In all patients, demographic, anthropometric data, biochemical, metabolic and hormonal data, and 24-h ambulatory blood pressure monitoring were assessed. Mild autonomous cortisol secretions (MACS) were diagnosed in patients without signs and symptoms of overt Cushing's syndrome and post dexamethasone (DXM) plasma cortisol concentration > 50 nmol/L (>1.8 μg/dL). Patients with overnight DXM-1 mg test positive showed higher values of diastolic blood pressure, glycemia and uric acid levels compared to patients with negative DXM test at baseline. During follow-up, the potential development of MACS in patients with nonfunctional AI showed a prevalence of 29%, though the cardiovascular and metabolic alterations were less pronounced compared to those diagnosed with MACS at baseline. Therefore, follow-ups with AI patients are useful for observing changes in clinical features. [ABSTRACT FROM AUTHOR]

Published
2024
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27. Cervical Spondylotic Myelopathy with Ossification of Posterior Longitudinal Ligament: Which Is the Most Suitable Surgical Procedure? A Technical Note

Author

Edvin Zekaj, Christian Saleh, Andrea Franzini, Andrea Ciuffi, and Domenico Servello

Subjects
anterior cervical discectomy, selective wedge corpectomy, anterior spinal cord compression, Surgery, RD1-811
Abstract

Introduction: Anterior cervical discectomy and fusion (ACDF) and anterior cervical corpectomy and fusion (ACCF) are widely performed to resolve anterior cervical spine compression. The main purpose of the different surgical techniques is to obtain an adequate decompression of the spinal cord and nerve roots, preserving spinal stability like in oblique corpectomy or leading to a final solid construct to achieve arthrodesis. Technical Note: We describe a surgical procedure for treating cervical spondylotic myelopathy(CSM) with ossification of the posterior longitudinal ligament (OPLL) at the level of C3-C4 and C4-C5. A double level discectomy C3-C4 and C4-C5 and selective posterior wedge corpectomy of C3, C4, and C5 were performed. Two cages (Zero-P VA) at C3-C4 and C4-C5 were positioned to obtain segmental stability and arthrodesis. An extended anterior cervical canal decompression was obtained and confirmed by postsurgical CT scan. At 15 months, dynamic X-ray showed fusion, and cervical magnetic resonance imaging (MRI) showed evidence of spinal canal decompression. Conclusions: Anterior cervical discectomy followed by selective wedge corpectomy appears to be a safe and effective technique for anterior spinal cord compression extending above and below the intervertebral disc space.

Published
2021
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30. Understanding Factors Associated With Psychomotor Subtypes of Delirium in Older Inpatients With Dementia

Author

Rispoli, Vincenzo, Malara, Alba, Spadea, Fausto, Di Cello, Serena, Ceravolo, Francesco, Fabiano, Francesco, Chiaradia, Giuseppe, Gabriele, Amedeo, Lenino, Peluso, Andrea, Taristano, Settembrini, Vincenzo, Capomolla, Domenico, Citrino, Antonella, Scriva, Antonietta, Bruno, Irene, Secchi, Roberto, De Martino, Eugenio, Muccinelli, Roberto, Lupi, Gerardo, Paonessa, Patrizio, Fabbri, Andrea, Passuti, Maria Teresa, Castellari, Sofia, Po, Andrea, Gaggioli, Guido, Varesi, Massimo, Moneti, Paolo, Capurso, Sebastiano, Latini, Vincenzo, Ghidotti, Stefano, Riccardelli, Francesco, Macchi, Maurizio, Rigo, Rosaria, Claudio, Pascale, Angelo, Bosio, Flavio, Cerrato, Benedetta, Bardelli, Boffelli, Stefano, Cassinadri, Angela, Franzoni, Simone, Spazzini, Elena, Andretto, Daniela, Tonini, Gabriele, Andreani, Laura, Coralli, Mirco, Balotta, Antonio, Cancelliere, Roberto, Ballardini, Giorgio, Simoncelli, Margherita, Mancini, Annarita, Strazzacapa, Mara, Fabio, Stefano, De Filippi, Francesco, Giudice, Chiara, Dentizzi, Cosimo, Azzini, Margherita, Cazzadori, Marco, Mastroeni, Valeria, Bertassello, Paolo, Claudia Benati, Helena Santana, Nesta, Elisa, Tobaldini, Chiara, Guerini, Fabio, Elena, Tambara, Mombelloni, Paolo, Fontanini, Fulvio, Gabriella, Lassa, Pizzorni, Crosio, Oliverio, Martina, Del Grosso, Luciano Luca, Giavedoni, Cristina, Bidoli, Giuliano, Mazzei, Bruno, Corsonello, Andrea, Fusco, Sergio, Vena, Silvio, De Vuono, Tommaso, Maiuri, Giorgio, Luca, Fimognari Filippo, Andrea, Arone, Giovanni, Sgrò, Rossella, Nicolazzo, Castegnaro, Eugenio, De Rosa, Salvatore, Sechi, Rossella Bazzano, Benvenuti, Enrico, Del Lungo, Ilaria, Giardini, Sante, Giulietti, Chiara, Mauro, Di Bari, Eleonora, Barghini, Martina, Paoli, Irene, Fiordelli, Riccardo, Barucci, Federica, Sgrilli, Ilaria, Del Lungo, Bertoletti, Erik, D'Amico, Ferdinando, Caronzolo, Francesco, Grippa, Alessandro, Lombardo, Giuseppina, Pipicella, Tiziana, Antonino, Saitta, Francesco, Corica, Valeria, Prestipino Giarritta, Daniela, Larosa, Domenico, Cucinotta, Giorgio, Basile, March, Albert, Nitti, Maria Teresa, Felici, Alessandro, Pavan, Silvia, Piazzani, Fabrizio, Lunelli, Alessandra, Dimori, Sergio, Margotta, Alessandro, Soglia, Tiziano, Postacchini, Demetrio, Brunelli, Roberto, Santini, Silvia, Francavilla, Monia, Macchiati, Ilenia, Sorvillo, Francesca, Giuli, Cinzia, Mecocci, Patrizia, Longo, Annalisa, Perticone, Francesco, Addesi, Desireè, Rosa, Paola Cerra, Bencardino, Giuseppe, Falbo, Tania, Grillo, Nadia, Marco, Filice, Mirella, Flippo, Fantò, Fausto, Isaia, Gianluca, Pezzilli, Stella, Bergamo, Daniele, Furno, Elisabetta, Rrodhe, Sokol, Lucarini, Simonetta, Dijk, Babette, Dall'Acqua, Francesca, Cappelletto, Francesco, Calvani, Donatella, Becheri, Dimitri, Giuseppe, Mottino, Costanza, Mitidieri, Vito, Antonio, Francesca, Bartalucci, Magherini, Lorenza, Novella, Malin, Franca, Boni, Lucia Gambardella, Paoli Martina, Valente, Carlo, Ilaria, Bracali, Alice, Foschini, Bo, Mario, Porrino, Paola, Ceci, Giacomo, Giuliana, Bottignole, Michela, Tibaldi, Eleonora, Coppo, Ettore, Evaristo, Camellini, Cinzia, Servello, Adriana, Grassi, Alessandro, Rozzini, Renzo, Tironi, Sara, Grassi, Maria Grazia, Troisi, Elio, Carlo, Caltagirone, Simona Gabriella, Di Santo, Flaminia, Franchini, Federica, Ratto, Beatrice, Pavoni, Sofia, Toniolo, Gabutto, Anna, Quazzo, Loredana, Rosatello, Annalisa, Suraci, Domenico, Tagliabue, Benedetta, Perrone, Chiara, Ferrara, Lucia, Castagna, Alberto, Tremolada, Maria Luisa, Giuseppe, Castoldi, Stefano, Barbero, Davide, Oltramonti, Piano, Simonetta, Serviddio, Gaetano, Lo Buglio, Aurelio, Gurrera, Tiziana, Merlo, Valeria, Rovai, Carla, Cotroneo, Antonino Maria, Carlucci, Rosaria, Abbaldo, Anna, Monzani, Fabio, Qasem, Ahmad Amedeo, Bini, Giacomo, Tafuto, Silvia, Galli, Giovanni, Bruni, Amalia Cecilia, Mancuso, Giovanna, Mancuso, Gerardo, Calipari, Daniela, Giuseppe Massimiliano, De Luca, Bernardini, Bruno, Corsini, Carla, Michele, Ciccarelli, Sara, Dal Farra, Cagnin, Annachiara, Fragiacomo, Federica, Pompanin, Sara, Piero, Amodio, Marco, Cagnin, Zurlo, Amedeo, Guerra, Gianluca, Pala, Marco, Menozzi, Luca, Gatti, Chiara Delli, Magon, Stefania, Roberto, Manfredini, Alfredo, De Giorgi, Fabio, Fabbian, Ruana, Tiseo, Elisa, Misurati, Benedetta, Boari, Christian, Molino, Marco, Pala, Massimo, Gallerani, Di Francesco, Vincenzo, Faccioli, Silvia, Pellizzari, Luca, Giorgia, Fontana, Barbagallo, Giuseppe, Lunardelli, Maria Lia, Martini, Emilio, Ferrari, Eleonora, Macchiarulo, Maria, Corneli, Maria, Bacci, Monica, Battaglia, Giuseppe, Anastasio, Luigi, Lo Storto, Mario Sostene, Seresin, Chiara, Simonato, Matteo, Loreggian, Michele, Cestonaro, Fausta, Durando, Mario, Latella, Raffaele, Mazzoleni, Marta, Russo, Giuseppe, Ponte, Martino, Valchera, Alessandro, Salustri, Giuseppe, Petritola, Donatella, Costa, Alfredo, Sinforiani, Elena, Cotta, Matteo Ramusino, Pizio, Renato Nicola, Cester, Alberto, Formilan, Marino, Pietro, Bonometto, Carbone, Pasqualina, 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Raffaele Antonelli, Bartoli, Isaura Rossi, Pluderi, Alice, Maina, Antonella, Pecoraro, Elisabetta, Sciarra, Michela, Prudente, Angela, Paola, Maina, Francesca, Mete, Manuel, Ventura, Luisella, Cesari, Maria, Pernigotti Luigi, Tina, Santangelo, Benini, Lucia, Levato, Francesco, Mhiuta, Victor, Alius, Florin, Davidoaia, Diana, Giardini, Vittorio, Garancini, Mattia, Bellamoli, Claudio, Terranova, Luciano, Bozzini, Claudia, Tosoni, Paolo, Provoli, Emma, Cascone, Luisa, Dioli, Andrea, Ferrarin, Gianfranco, Bucci, Adelmo, Bua, Guido, Fenu, Sara, Bianchi, Giovanna, Casella, Silvia, Romano, Valentina, Maurizio, Poli, Mascherona, Ilenia, Belotti, Gloria, Cavaliere, Sabina, Cuni, Estella, Merciuc, Nina, Oberti, Rosella, Veneziani, Silvia, Capoferri, Emanuela, De Bernardi, Elisabetta, Colombo, Katia, Bravi, Marco, Nicoletta, Negrinotti, D'Arcangelo, Paolo, Montenegro, Nicola, Montanari, Roberto, Lamanna, Pierpaolo, Gasperini, Beatrice, Isabella, Montesi, Stefania, Diotallevi, Gaia, Altobelli, 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Elisabetta, Tomassini, Pia Francesca, Del Gobbo, Maurizio, Ugenti, Francesca, Romeo, Pasquale, Nardelli, Anna, Lauretani, Fulvio, Visioli, Sandra, Montanari, Ilaria, Ermini, Francesca, Giordano, Antonio, Pigato, Giorgio, Simeone, Emilio, Barbujani, Mario, Giampieri, Marina, Amoruso, Raffaele, Piccinini, Maristella, Ferrari, Camilla, Gambetti, Claudio, Sfrappini, Mario, Semeraro, Letizia, Striuli, Rinaldo, Mariani, Claudia, Pelliccioni, Giuseppe, Marinelli, Donatella, Fabi, Katia, Rossi, Tommaso, Pesallaccia, Martina, Sabbatini, Debora, Gobbi, Beatrice, Cerqua, Raffaella, Tagliani, Giancarla, Schlauser, Elena, Caser, Luciano, Caramello, Elisa, Sandigliano, Franca, Rosso, Giorgio, Ferrari, Alberto, Bendini, Chiara, Luisa, Davoli Maria, Casella, Monica, Prampolini, Raffaella, Scevola, Moreno, Vitale, Enrico, Roberto, Brugiolo, Carlo, Fagherazzi, Sergio, Fusco, Alberto, Scarpa, Daniela, Zara, Giulia, Bertozzo, Serena, Granziera, Michele, Barazzuol, Maugeri, Domenico, Sorace, Rosaria, Anzaldi, Massimiliano, De Gesu, Roberto, Morrone, Giuseppe, Davolio, Federica, Fabbo, Andrea, Palmieri, Marina, Zoli, Marco, Forti, Paola, Pirazzoli, Luca, Fabbri, Elisa, Terenzi, Laura, Bergolari, Federica, Wenter, Christian, Ruffini, Ingrid, Insam, Miriam, Abraham, Elisabeth, Kirchlechner, Christine, Cucinotta, Domenico, Antonino, Lasco, Basile, Giorgio, Grazia, Arena Maria, Parise, Pasquale, Boccali, Andrea, Amici, Serena, Gambacorta, Maurizia, Lasagni, Anna, Lovati, Roberto, Giovinazzo, Francesca, Kimak, Elzbieta, Zappa, Paolo, Medici, Francesco, Lo Castro, Marika, Mauro, Flavia, De Luca, Alessandro, Sancesario, Giuseppe, Martorana, Alessandro, Scaricamazza, Beatrice, Toniolo, Sofia, Di Lorenzo, Francesco, Liguori, Claudio, Lasco, Antonino, Vita, Natale, Giomi, Mirna, Forte, Floriana, Padovani, Alessandro, Rozzini, Luca, Ceraso, Anna, Salvatore, Caratozzolo, Cottino, Maria, Vitali, Silvia, Marelli, Eleonora, Tripi, Gabriele, Miceli, Salvatore, Urso, Giovanni, Grioni, Giuseppe, Vezzadini, Giuliana, Misaggi, Giulia, Forlani, Chiara, Avanzi, Stefano, Serena, Saulle, Claudia, Casarini, Marilena, Viggiano, Alberto, Luisa, Diego, Ghianda, Alessandro, Giordano, Iemolo, Francesco, Giordano, Antonello, Sanzaro, Enzo, D'Asta, Gabriele, Proietto, Maria, Carnemolla, Anna, Razza, Grazia, Spadaro, Daniela, Bertolotti, Marco, Mussi, Chiara, Neviani, Francesca, Roberto, Chiesa, Valentina, Guerzoni, Linda, Morselli, Francesca, Venturelli, Tarozzi, Adriano, Balestri, Francesca, Mannarino, Giulio, Bigolari, Michela, Natale, Alessia, Grassi, Simona, Bottaro, Cinzia, Stefanelli, Sara, Bovone, Ugo, Tortorolo, Umberto, Quadri, Roberto, Leone, Giuseppe, Ponzetto, Maria, Frasson, Paola, Annoni, Giorgio, Bellelli, Giuseppe, Bruni, Adriana, Confalonieri, Roberto, Corsi, Maurizio, Moretti, Daniela, Teruzzi, Fabiola, Umidi, Simona, Mazzola, Paolo, Perego, Sabrina, Persico, Ilaria, Olivieri, Giulia, Bonfanti, Alessandra, Hajnalka, Szabò, Galeazzi, Marianna, Massariello, Francesca, Anzuini, Alessandra, Caffarra, Paolo, Barocco, Federica, Spallazzi, Marco, Paolo, Ceda Gian, Simonetta, Morganti, Andrea, Artoni, Chioatto, Paolo, Bortolamei, Sandra, Soattin, Lucia, Ruotolo, Giovanni, Beneamino, Borelli, Giuseppe, Barilaro, Bertazzoli, Marco, Rota, Elisabetta, Adobati, Annamaria, Scarpa, Alberto, Granziera, Serena, Zuccher, Paola, Fabbro, Angela Dal, Zara, Daniela, Lo Nigro, Ambra, Franchetti, Lorena, Toniolo, Marika, Marcuzzo, Cinzia, Rollone, Marco, Guerriero, Fabio, Sgarlata, Carmelo, Massè, Alessandro, Zatti, Giovanni, Piatti, Massimiliano, Graci, Jole, Benati, Giuseppe, Boschi, Federica, Biondi, Mario, Fiumi, Nicoletta, Erika, Tamburini, Locatelli, Sergio Mario, Mauri, Sabrina, Beretta, Mauro, Margheritis, Laura, Desideri, Giovanbattista, Liberatore, Ester, Carucci, Anna Cecilia, Bonino, Paolo, Caput, Margherita, Antonietti, Maria Paola, Polistena, Giuseppe, De la Pierre, Franz, Mari, Marcello, Massignani, Paola, Tombesi, Fabio, Selvaggio, Fabio, Verbo, Brunella, Bodoni, Paolo, Marchionni, Niccolò, Mossello, Enrico, Sabatini, Tony, Mussio, Eleonora, Magni, Eugenio, Bianchetti, Angelo, Crucitti, Andrea, Titoldini, Giulia, Cossu, Beatrice, Fascendini, Sara, Licini, Cristina, Tomasoni, Angela, Calderazzo, Massimo, Daniela, Tropiano, Valentina, Laganà, Melotti, Rita Maria, Lilli, Albina, Buda, Simona, Adversi, Marco, Noro, Gabriele, Turco, Renato, Ubezio, M Chiara, Mantovani, Anna Roberta, Viola, Maria Cristina, Serrati, Carlo, Pretta, Stefano, Infante, Maria, Gentile, Simona, Morandi, Alessandro, D'Ambrosio, Viviana, Mazzanti, Paolo, Brambilla, Cristina, Sportelli, Silvia, Platto, Caterina, Faraci, Bianca, Quattrocchi, Daniela, Pernigotti, Luigi Maria, Pisu, Cristina, Sicuro, Francesca, Zagnoni, Piergiuseppe, Ghiglia, Stefania, Mosca, Massimiliano, Corazzin, Ileana, Deola, Mariangela, Biagini, Carlo Adriano, Bencini, Francesca, Cantini, Claudia, Tonon, Elisabetta, Pierinelli, Silvia, Onofrj, Marco, Thomas, Astrid, Filomena, Borbone, Bonanni, Laura, Gabriella, Cacchiò, Comi, Giancarlo, Magnani, Giuseppe, Santangelo, Roberto, Mazzeo, Salvatore, Giuseppe, Magnani, Francesca, Caso, Giordano, Cecchetti, Roberto, Sant'Angelo, Barbieri, Cristina, Giroldi, Liviana, Bandini, Fabio, Masina, Marco, Malservisi, Simona, Cicognani, Annalena, Ricca, Laura, Piccininni, Maristella, Tassinari, Tiziana, Brogi, Davide, Sugo, Annalisa, Alessandra, Filippi, Sonia, Manfredi, Valerio, Vanni, Andrea, Usai Carlo, Enrico, Colombo, Vera, Renna Francesca, Assunta, Sauchella, Gianmaria, Zavarise, Mauro, Prete, Pietro, Bisagni, Roberto, Masini, Salvatore, Carrabetta, Barone, Antonella, Razzano, Monica, Giuseppe, Imperoli, Angela, Bini, Francesco, Serra, Valeria, D'Agostino, Federico, Gianolla, Lucia, Pietrangeli, Antonella, Velardi, Elisabetta, Di Cello, Cristina, Rosati, Nadia, Casali, Maria, Sessa, Luciano, Abruzzi, Chiara, Costanzi, Bini, Paolo, Pignata, Michele, Enrico, Buonagurio, Maria, Vollery, Giovanni, Carrieri, Giorgio, Cioni, Andrea, Toschi, Marco, Metra, Piera, Ranieri, Alberto, Zucchelli, Ceccon, Anna, Magrin, Laura, Marin, Sara, Barbara, Sofia, Marco, Masina, Laura, Ghedini, Matteo, Moroni, Marco, Paragona, Caterina, Pallotti Maria, Carla, Rota, Federica, Gottardi, Clara, Tomasoni, Melania, Cappuccio, Giampaolo, Bonini, Stefano, Guerini, Valeria, Guerini, Lucia, Merla, Giovambattista, Desideri, Ester, Liberatore, Cecilia, Carucci Anna, Maurizio, Tovaglieri, Vera, Renna Frandesca, Nadia, Bongiorni, Grillo, Antonio, Arenare, Francesca, Tonino, Mineo, David, Kanah, Giorgio, Vianello Piero, Ubaldo, Balducci, Vincenzo, Sidoti, Stefano, Montanari, Marino, Formilan, Busonera Flavio, Murelli Teodolindo, Paolo, Albanese, Monica, Maselli, Francesco, Bolzetta, Roberto, Fabris, Paolo, Bonino, DuranteMangoni, Emanuele, Testoni, Michela, Fabio, Di Stefano, Loredana, Seccia, Valeria, Sonzini, Fabiano, Maria, Annabella, Di Giorgio, Salvatore, De Cosmo, Greco, Antonio, Grazia, D'Onofrio, Daniele, Sancarlo, Gianluca, Resta, Renzo, Girardello, Sergio, Minervini, Morena, Boni, Vitali, Mariagrazia, Marina, Pizzoni, Paolo, De Colle, Cristina, Scarpa, Alessandra, Frattola, Orlandini, Francesco, La Regina, Micaela, Desirée, Addesi, Mirella, Filippo, Mario, Bo, Paola, Porrino, Padulo, Filomena, Cristina, Margheriti, Dario, Rolano, Giancarla, Moscatelli, Guido, Radaelli, Elena, Montini, Prete, Cosimo, Marileda, Novello, Igor, Bramuzzo, Nicole, Bertin, Elena, Rinaldo, Paolillo, Ciro, Riccardi, Angela, Claudia, Benedetti, Barbara, Rizzi, Francesca, Montagna, Silvia, Vitali, Chiara, Cutaia, Oliver, Baca, Mauro, Colombo, Eleonora, Marelli, Giuseppe, Procino, Rosaria, Tararà, Maria, Cottino, Davide, Dell'Acqua, Stefania, Cislaghi, Marco, Cairati, Massimo, Porta, Luca, Scaglione, Martina, De Feo, Paola, Vertolli, Lia, Salvati, Sandro, Cinotti, Valentina, De Santis, Erminia, Biferi, Paola, Cheli, Romina, Rebizzo, Minisola, Salvatore, Luciano, Colangelo, Pasquale, Abete, Ilaria, Liguori, Francesca, Curcio, Guglielmo, Spassini, Marco, Engheben, Sara, Rotunno, Paola, Arosio, Claudio, Angelini, Francesco, Reggiani, Alessandro, Cappelli, Simona, Marcheselli, Lara, Fratticci, Paola, Ranzani, Simonetta, Cesarini, Antonella, Cerini, Generoso, Uomo, Fernando, Gallucci, Giuliano, Ceschia, Emanuela, Serra, Mariolina, Sola, Alessandro, Delitala, Chiara, Pes, Giulia, Lobianco, Alessandro, Giani, Famularo, Simone, Sandini, Marta, Pinotti, Enrico, Gianotti, Luca, Antonella, Battuello, Giulia, Pintore, Sante, Giardini, Rossi, Andrea, Rubele, Sofia, Sant, Selena, Marco, Vignati, Danila, Clerici, Fabio, Rosa, Bandirali, Maria Pia, Nicoletta, Cattaneo, Laura, Boffi, Paolo, Tosoni, Luciano, Terranova, Leonello, Avalli, Margherita, Scanziani, Stefania, De Notaris, Pierluigi, Del Santo, Laura, Rossi, Fabiana, Tezza, Giovanna, Cervati, Antonino, Andreolli, Felice, Catalano, Giuseppe, Burattin, Danilo, Fogli, Giovanna, Di Bella, Francesco, Landi, Salini, Sara, Angela, Brunetti Maria, Giorgetta, Cappa, Giovanni, Galvagno, Paola, Cena, Gerardo, Bruno, Silvio, Raspo, Letizia, Semeraro, Sabrina, Pedrotti, Davide, Barra, Rosaria, Rizzo Maria, Maria, Dalise Anna, Raffaele, Prestano, Palmieri, Vincenzo Ostilio, Palasciano, Giuseppe, Belfiore, Anna, Portincasa, Piero, Carlo, Sabbà, Vincenzo, Solfrizzi, Alessia, D'introno, Valiani, Vincenzo, Carolina, Bologna, Tiziana, Ciarambino, Paola, Turelli, Ugo, Pazzaglia, Federica, Rodella, Giacomo, Piana, Castellano, Maurizio, Anna, Garelli, Elisa, Casella, Federica, Campana, Antonietta, Coschignano Maria, Luigi, Marinangeli, Fabio, Lorico, Salvatore, Bazzano, Giuseppe, Menculini, Gelosa, Giorgio, Viviana, Ambrogio Teresa, Piras, Valentina, Andrea, Ciricugno, Alessandra, Bollari, Coen, Daniele, Magliola, Renata, Milanesio, Deborah, Muzzulini, Carlo Lorenzo, Paolo, Fogliacco, Marinella, Turla, Sofia, Cotelli Maria, Marta, Bianchi, Siano, Pietro, Capo, Giuseppe, Napoletano, Rosa, Cecilia, Politi, Mancini, Concetta, Del Buono, Corrado, De Bartolomeo, Giuseppe, Addolorata, Martinelli, Carmen, Cefalogli, Roberto, Cozzi, Giovanni, Virtuani Angelo, Moschettini, Gianfilippo, Franco, Mastroianni, Daniela, Roglia, D'Amico, Gabriella, Mirella, Palella, Endrizzi, Cristina, Trotta, Lucia, Ciarambino, Tiziana, Orazio, Zanetti, Emanuela, Terazzi, Marta, Sacchetti, Thomas, Fleetwood, Giacomo, Tondo, Ignazio, Di Fazio, Andrea, Bruni, Giuseppe, Orsitto, Emanuela, Fabbro, Serena, Amici, Elena, D'Imporzano, Anna, Casanova, Serena, Bertolio, Erika, Nervo, Roberto, Silvestri, Elena, Semproni, Manuela, Pintus, Francesca, Aloe, Angelo, Tagliaccica, Zambon, Antonella, and Di Santo, Simona G.

Published
2020
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32. The heat shock transcription factor HSF-1 protects Caenorhabditis elegans from peroxide stress

Author

Francesco A. Servello and Javier Apfeld

Subjects
C. elegans, Oxidative stress, Heat shock factor, Hormesis, Medicine
Abstract

Cells induce conserved defense mechanisms that protect them from oxidative stress. How these defenses are regulated in multicellular organisms is incompletely understood. Here, we show that the heat shock transcription factor HSF-1 protects the nematode Caenorhabditis elegans from the oxidative stress caused by environmental peroxide. In response to a heat shock or a mild temperature increase, HSF-1 protects the nematodes from subsequent peroxide stress in a manner that depends on HSF-1’s transactivation domain. At constant temperature, HSF-1 protects the nematodes from peroxide stress independently of its transactivation domain, likely by inducing the expression of asp-4/cathepsin and dapk-1/dapk. Thus, two distinct HSF-1-dependent processes protect C. elegans from peroxide stress.

Published
2020
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35. Towards a common language in neurosurgical outcome evaluation: the NEON (NEurosurgical Outcome Network) proposal

Author

Ferroli, Paolo, Schiavolin, Silvia, Mariniello, Arianna, Acerbi, Francesco, Restelli, Francesco, Schiariti, Marco, LA Corte, Emanuele, Falco, Jacopo, Levi, Vincenzo, Dimeco, Francesco, Assietti, Roberto, Bongetta, Daniele, V Colombo, Elena, Bellocchi, Silvio, Sangiorgi, Simone, Bistazzoni, Simona, Polosa, Maria, I Orru, Maria, Spena, Giannantonio, Bernucci, Claudio, M Sicignano, Angelo, Fanti, Andrea, Brembilla, Carlo, Resmini, Bruno, Costi, Emanuele, Cenzato, Marco, Talamonti, Giuseppe, Bottini, Gabriella, Scarpa, Pina, Bollani, Alessandra, Querzola, Matteo, Palmas, Giulio, DE Gonda, Federico, Bosio, Lorenzo, Egidi, Marcello, Tardivo, Valentina, Fioravanti, Antonio, Subacchi, Sara, Fontanella, Marco, Biroli, Antonio, Cereda, Claudio, Paolo Panciani, Pier, Bergomi, Riccardo, Pertichetti, Marta, Tancioni, Flavio, Bona, Alberto, A Tartara, Fulvio, Fornari, Maurizio, Pessina, Federico, Lasio, Giovanni, Cardia, Andrea, Servadei, Franco, Riva, Marco, Casarotti, Alessandra, Giussani, Carlo, Fiori, Leonardo, Mazzoleni, Fabio, Vaiani, Simona, Carrabba, Giorgio, DI Cristofori, Andrea, P Sganzerla, Erik, Vimercati, Alberto, Isella, Valeria, Mauri, Ilaria, Incerti, Michele, Sicuri, Giovanni, Miramonti, Valentina, Stefini, Roberto, Spagnoli, Diego, Piparo, Maurizio, Grimod, Gianluca, Regazzoni, Rossana, Vismara, Daniela, Mazzeo, Lucio, Monti, Emanuele, Franzin, Alberto, Vivaldi, Oscar, Maietti, Alessandra, Pini, Elisa, Servello, Domenico, Zekaj, Edvin, DE Michele, Sara, Locatelli, Marco, Borsa, Stefano, Grimoldi, Nadia, Caroli, Manuela, Tariciotti, Leonardo, Abete-Fornara, Giorgia, Vitale, Mario, Leonardi, Matilde, Broggi, Morgan, Paolo Ferroli, Silvia Schiavolin, Arianna Mariniello, Francesco Acerbi, Francesco Restelli, Marco Schiariti, Emanuele LA Corte, Jacopo Falco, Vincenzo Levi, Francesco Dimeco, Roberto Assietti, Daniele Bongetta, Elena V Colombo, Silvio Bellocchi, Simone Sangiorgi, Simona Bistazzoni, Maria Polosa, Maria I Orru, Giannantonio Spena, Claudio Bernucci, Angelo M Sicignano, Andrea Fanti, Carlo Brembilla, Bruno Resmini, Emanuele Costi, Marco Cenzato, Giuseppe Talamonti, Gabriella Bottini, Pina Scarpa, Alessandra Bollani, Matteo Querzola, Giulio Palmas, Federico DE Gonda, Lorenzo Bosio, Marcello Egidi, Valentina Tardivo, Antonio Fioravanti, Sara Subacchi, Marco Fontanella, Antonio Biroli, Claudio Cereda, Pier Paolo Panciani, Riccardo Bergomi, Marta Pertichetti, Flavio Tancioni, Alberto Bona, Fulvio A Tartara, Maurizio Fornari, Federico Pessina, Giovanni Lasio, Andrea Cardia, Franco Servadei, Marco Riva, Alessandra Casarotti, Carlo Giussani, Leonardo Fiori, Fabio Mazzoleni, Simona Vaiani, Giorgio Carrabba, Andrea DI Cristofori, Erik P Sganzerla, Alberto Vimercati, Valeria Isella, Ilaria Mauri, Michele Incerti, Giovanni Sicuri, Valentina Miramonti, Roberto Stefini, Diego Spagnoli, Maurizio Piparo, Gianluca Grimod, Rossana Regazzoni, Daniela Vismara, Lucio Mazzeo, Emanuele Monti, Alberto Franzin, Oscar Vivaldi, Alessandra Maietti (ORCID:0000-0002-9819-7037), Elisa Pini, Domenico Servello, Edvin Zekaj, Sara DE Michele, Marco Locatelli, Stefano Borsa, Nadia Grimoldi, Manuela Caroli, Leonardo Tariciotti, Giorgia Abete-Fornara, Mario Vitale, Matilde Leonardi, Morgan Broggi, Ferroli, Paolo, Schiavolin, Silvia, Mariniello, Arianna, Acerbi, Francesco, Restelli, Francesco, Schiariti, Marco, LA Corte, Emanuele, Falco, Jacopo, Levi, Vincenzo, Dimeco, Francesco, Assietti, Roberto, Bongetta, Daniele, V Colombo, Elena, Bellocchi, Silvio, Sangiorgi, Simone, Bistazzoni, Simona, Polosa, Maria, I Orru, Maria, Spena, Giannantonio, Bernucci, Claudio, M Sicignano, Angelo, Fanti, Andrea, Brembilla, Carlo, Resmini, Bruno, Costi, Emanuele, Cenzato, Marco, Talamonti, Giuseppe, Bottini, Gabriella, Scarpa, Pina, Bollani, Alessandra, Querzola, Matteo, Palmas, Giulio, DE Gonda, Federico, Bosio, Lorenzo, Egidi, Marcello, Tardivo, Valentina, Fioravanti, Antonio, Subacchi, Sara, Fontanella, Marco, Biroli, Antonio, Cereda, Claudio, Paolo Panciani, Pier, Bergomi, Riccardo, Pertichetti, Marta, Tancioni, Flavio, Bona, Alberto, A Tartara, Fulvio, Fornari, Maurizio, Pessina, Federico, Lasio, Giovanni, Cardia, Andrea, Servadei, Franco, Riva, Marco, Casarotti, Alessandra, Giussani, Carlo, Fiori, Leonardo, Mazzoleni, Fabio, Vaiani, Simona, Carrabba, Giorgio, DI Cristofori, Andrea, P Sganzerla, Erik, Vimercati, Alberto, Isella, Valeria, Mauri, Ilaria, Incerti, Michele, Sicuri, Giovanni, Miramonti, Valentina, Stefini, Roberto, Spagnoli, Diego, Piparo, Maurizio, Grimod, Gianluca, Regazzoni, Rossana, Vismara, Daniela, Mazzeo, Lucio, Monti, Emanuele, Franzin, Alberto, Vivaldi, Oscar, Maietti, Alessandra, Pini, Elisa, Servello, Domenico, Zekaj, Edvin, DE Michele, Sara, Locatelli, Marco, Borsa, Stefano, Grimoldi, Nadia, Caroli, Manuela, Tariciotti, Leonardo, Abete-Fornara, Giorgia, Vitale, Mario, Leonardi, Matilde, Broggi, Morgan, Paolo Ferroli, Silvia Schiavolin, Arianna Mariniello, Francesco Acerbi, Francesco Restelli, Marco Schiariti, Emanuele LA Corte, Jacopo Falco, Vincenzo Levi, Francesco Dimeco, Roberto Assietti, Daniele Bongetta, Elena V Colombo, Silvio Bellocchi, Simone Sangiorgi, Simona Bistazzoni, Maria Polosa, Maria I Orru, Giannantonio Spena, Claudio Bernucci, Angelo M Sicignano, Andrea Fanti, Carlo Brembilla, Bruno Resmini, Emanuele Costi, Marco Cenzato, Giuseppe Talamonti, Gabriella Bottini, Pina Scarpa, Alessandra Bollani, Matteo Querzola, Giulio Palmas, Federico DE Gonda, Lorenzo Bosio, Marcello Egidi, Valentina Tardivo, Antonio Fioravanti, Sara Subacchi, Marco Fontanella, Antonio Biroli, Claudio Cereda, Pier Paolo Panciani, Riccardo Bergomi, Marta Pertichetti, Flavio Tancioni, Alberto Bona, Fulvio A Tartara, Maurizio Fornari, Federico Pessina, Giovanni Lasio, Andrea Cardia, Franco Servadei, Marco Riva, Alessandra Casarotti, Carlo Giussani, Leonardo Fiori, Fabio Mazzoleni, Simona Vaiani, Giorgio Carrabba, Andrea DI Cristofori, Erik P Sganzerla, Alberto Vimercati, Valeria Isella, Ilaria Mauri, Michele Incerti, Giovanni Sicuri, Valentina Miramonti, Roberto Stefini, Diego Spagnoli, Maurizio Piparo, Gianluca Grimod, Rossana Regazzoni, Daniela Vismara, Lucio Mazzeo, Emanuele Monti, Alberto Franzin, Oscar Vivaldi, Alessandra Maietti (ORCID:0000-0002-9819-7037), Elisa Pini, Domenico Servello, Edvin Zekaj, Sara DE Michele, Marco Locatelli, Stefano Borsa, Nadia Grimoldi, Manuela Caroli, Leonardo Tariciotti, Giorgia Abete-Fornara, Mario Vitale, Matilde Leonardi, and Morgan Broggi

Abstract

BACKGROUNDː To achieve a consensus on the minimum set of outcome measures and predictors to be used in the neurosurgical practice and on the timing of outcome assessment. METHODSː A consensus building approach was employed. All neurosurgical departments in Lombardy (Italy) were invited to participate by the Fondazione IRCCS Istituto Neurologico Carlo Besta. Three workshops were organized during which a multidisciplinary group called Neurosurgical Outcome Network (NEON) was created and the methodology to select outcome measures, predictors, and timing of outcome assessment was established. Eight working groups were created for the different neurosurgical diseases (neuro-oncological, skull base, vascular, traumatic, spinal, peripheral nervous system, malformation, functional) and 8 workshops were organized to identify the outcome measures and predictors specific for each of the neurosurgical diseases based on the experts’ clinical practice and the existing literature. RESULTSː A total of 20 neurosurgical departments participated in this study. Specific outcome measures, predictors and the timing of outcome assessment were identified for each of the 8 neurosurgical diseases. Moreover, a list of variables common to all pathologies were identified by the NEON group as further data to be collected. CONCLUSIONSː A consensus on the minimum set of outcome measures and predictors and the timing of outcome assessments for 8 neurosurgical diseases was achieved by a group of neurosurgeons of the Lombardy region, called NEON. These sets could be used in future studies for a more homogeneous data collection and as a starting point to reach further agreement also at national and international level.

Published
2023

36. Towards a common language in neurosurgical outcome evaluation: the NEON (NEurosurgical Outcome Network) proposal

Author

Ferroli, P, Schiavolin, S, Mariniello, A, Acerbi, F, Restelli, F, Schiariti, M, LA Corte, E, Falco, J, Levi, V, Dimeco, F, Assietti, R, Bongetta, D, Colombo, E, Bellocchi, S, Sangiorgi, S, Bistazzoni, S, Polosa, M, Orru, M, Spena, G, Bernucci, C, Sicignano, A, Fanti, A, Brembilla, C, Resmini, B, Costi, E, Cenzato, M, Talamonti, G, Bottini, G, Scarpa, P, Bollani, A, Querzola, M, Palmas, G, DE Gonda, F, Bosio, L, Egidi, M, Tardivo, V, Fioravanti, A, Subacchi, S, Fontanella, M, Biroli, A, Cereda, C, Panciani, P, Bergomi, R, Pertichetti, M, Tancioni, F, Bona, A, Tartara, F, Fornari, M, Pessina, F, Lasio, G, Cardia, A, Servadei, F, Riva, M, Casarotti, A, Giussani, C, Fiori, L, Mazzoleni, F, Vaiani, S, Carrabba, G, DI Cristofori, A, Sganzerla, E, Vimercati, A, Isella, V, Mauri, I, Incerti, M, Sicuri, G, Miramonti, V, Stefini, R, Spagnoli, D, Piparo, M, Grimod, G, Regazzoni, R, Vismara, D, Mazzeo, L, Monti, E, Franzin, A, Vivaldi, O, Maietti, A, Pini, E, Servello, D, Zekaj, E, DE Michele, S, Locatelli, M, Borsa, S, Grimoldi, N, Caroli, M, Tariciotti, L, Abete-Fornara, G, Vitale, M, Leonardi, M, Broggi, M, Ferroli, Paolo, Schiavolin, Silvia, Mariniello, Arianna, Acerbi, Francesco, Restelli, Francesco, Schiariti, Marco, LA Corte, Emanuele, Falco, Jacopo, Levi, Vincenzo, Dimeco, Francesco, Assietti, Roberto, Bongetta, Daniele, Colombo, Elena V, Bellocchi, Silvio, Sangiorgi, Simone, Bistazzoni, Simona, Polosa, Maria, Orru, Maria I, Spena, Giannantonio, Bernucci, Claudio, Sicignano, Angelo M, Fanti, Andrea, Brembilla, Carlo, Resmini, Bruno, Costi, Emanuele, Cenzato, Marco, Talamonti, Giuseppe, Bottini, Gabriella, Scarpa, Pina, Bollani, Alessandra, Querzola, Matteo, Palmas, Giulio, DE Gonda, Federico, Bosio, Lorenzo, Egidi, Marcello, Tardivo, Valentina, Fioravanti, Antonio, Subacchi, Sara, Fontanella, Marco, Biroli, Antonio, Cereda, Claudio, Panciani, Pier Paolo, Bergomi, Riccardo, Pertichetti, Marta, Tancioni, Flavio, Bona, Alberto, Tartara, Fulvio A, Fornari, Maurizio, Pessina, Federico, Lasio, Giovanni, Cardia, Andrea, Servadei, Franco, Riva, Marco, Casarotti, Alessandra, Giussani, Carlo, Fiori, Leonardo, Mazzoleni, Fabio, Vaiani, Simona, Carrabba, Giorgio, DI Cristofori, Andrea, Sganzerla, Erik P, Vimercati, Alberto, Isella, Valeria, Mauri, Ilaria, Incerti, Michele, Sicuri, Giovanni, Miramonti, Valentina, Stefini, Roberto, Spagnoli, Diego, Piparo, Maurizio, Grimod, Gianluca, Regazzoni, Rossana, Vismara, Daniela, Mazzeo, Lucio, Monti, Emanuele, Franzin, Alberto, Vivaldi, Oscar, Maietti, Alessandra, Pini, Elisa, Servello, Domenico, Zekaj, Edvin, DE Michele, Sara, Locatelli, Marco, Borsa, Stefano, Grimoldi, Nadia, Caroli, Manuela, Tariciotti, Leonardo, Abete-Fornara, Giorgia, Vitale, Mario, Leonardi, Matilde, Broggi, Morgan, Ferroli, P, Schiavolin, S, Mariniello, A, Acerbi, F, Restelli, F, Schiariti, M, LA Corte, E, Falco, J, Levi, V, Dimeco, F, Assietti, R, Bongetta, D, Colombo, E, Bellocchi, S, Sangiorgi, S, Bistazzoni, S, Polosa, M, Orru, M, Spena, G, Bernucci, C, Sicignano, A, Fanti, A, Brembilla, C, Resmini, B, Costi, E, Cenzato, M, Talamonti, G, Bottini, G, Scarpa, P, Bollani, A, Querzola, M, Palmas, G, DE Gonda, F, Bosio, L, Egidi, M, Tardivo, V, Fioravanti, A, Subacchi, S, Fontanella, M, Biroli, A, Cereda, C, Panciani, P, Bergomi, R, Pertichetti, M, Tancioni, F, Bona, A, Tartara, F, Fornari, M, Pessina, F, Lasio, G, Cardia, A, Servadei, F, Riva, M, Casarotti, A, Giussani, C, Fiori, L, Mazzoleni, F, Vaiani, S, Carrabba, G, DI Cristofori, A, Sganzerla, E, Vimercati, A, Isella, V, Mauri, I, Incerti, M, Sicuri, G, Miramonti, V, Stefini, R, Spagnoli, D, Piparo, M, Grimod, G, Regazzoni, R, Vismara, D, Mazzeo, L, Monti, E, Franzin, A, Vivaldi, O, Maietti, A, Pini, E, Servello, D, Zekaj, E, DE Michele, S, Locatelli, M, Borsa, S, Grimoldi, N, Caroli, M, Tariciotti, L, Abete-Fornara, G, Vitale, M, Leonardi, M, Broggi, M, Ferroli, Paolo, Schiavolin, Silvia, Mariniello, Arianna, Acerbi, Francesco, Restelli, Francesco, Schiariti, Marco, LA Corte, Emanuele, Falco, Jacopo, Levi, Vincenzo, Dimeco, Francesco, Assietti, Roberto, Bongetta, Daniele, Colombo, Elena V, Bellocchi, Silvio, Sangiorgi, Simone, Bistazzoni, Simona, Polosa, Maria, Orru, Maria I, Spena, Giannantonio, Bernucci, Claudio, Sicignano, Angelo M, Fanti, Andrea, Brembilla, Carlo, Resmini, Bruno, Costi, Emanuele, Cenzato, Marco, Talamonti, Giuseppe, Bottini, Gabriella, Scarpa, Pina, Bollani, Alessandra, Querzola, Matteo, Palmas, Giulio, DE Gonda, Federico, Bosio, Lorenzo, Egidi, Marcello, Tardivo, Valentina, Fioravanti, Antonio, Subacchi, Sara, Fontanella, Marco, Biroli, Antonio, Cereda, Claudio, Panciani, Pier Paolo, Bergomi, Riccardo, Pertichetti, Marta, Tancioni, Flavio, Bona, Alberto, Tartara, Fulvio A, Fornari, Maurizio, Pessina, Federico, Lasio, Giovanni, Cardia, Andrea, Servadei, Franco, Riva, Marco, Casarotti, Alessandra, Giussani, Carlo, Fiori, Leonardo, Mazzoleni, Fabio, Vaiani, Simona, Carrabba, Giorgio, DI Cristofori, Andrea, Sganzerla, Erik P, Vimercati, Alberto, Isella, Valeria, Mauri, Ilaria, Incerti, Michele, Sicuri, Giovanni, Miramonti, Valentina, Stefini, Roberto, Spagnoli, Diego, Piparo, Maurizio, Grimod, Gianluca, Regazzoni, Rossana, Vismara, Daniela, Mazzeo, Lucio, Monti, Emanuele, Franzin, Alberto, Vivaldi, Oscar, Maietti, Alessandra, Pini, Elisa, Servello, Domenico, Zekaj, Edvin, DE Michele, Sara, Locatelli, Marco, Borsa, Stefano, Grimoldi, Nadia, Caroli, Manuela, Tariciotti, Leonardo, Abete-Fornara, Giorgia, Vitale, Mario, Leonardi, Matilde, and Broggi, Morgan

Abstract

Background: The aim of this study was to achieve a consensus on the minimum set of outcome measures and predictors to be used in the neurosurgical practice and on the timing of outcome assessment. Methods: A consensus building approach was employed. All neurosurgical departments in Lombardy (Italy) were invited to participate by the Carlo Besta Neurologic Institute IRCCS Foundation. Three workshops were organized during which a multidisciplinary group called Neurosurgical Outcome Network (NEON) was created and the methodology to select outcome measures, predictors, and timing of outcome assessment was established. Eight working groups were created for the different neurosurgical diseases (neuro-oncological, skull base, vascular, traumatic, spinal, peripheral nervous system, malformation, functional) and 8 workshops were organized to identify the outcome measures and predictors specific for each of the neurosurgical diseases based on the experts' clinical practice and the existing literature. Results: A total of 20 neurosurgical departments participated in this study. Specific outcome measures, predictors and the timing of outcome assessment were identified for each of the 8 neurosurgical diseases. Moreover, a list of variables common to all pathologies were identified by the NEON group as further data to be collected. Conclusions: A consensus on the minimum set of outcome measures and predictors and the timing of outcome assessments for 8 neurosurgical diseases was achieved by a group of neurosurgeons of the Lombardy region, called NEON. These sets could be used in future studies for a more homogeneous data collection and as a starting point to reach further agreement also at national and international level.

Published
2023

40. Ablative brain surgery: an overview

Author

Andrea Franzini, Shayan Moosa, Domenico Servello, Isabella Small, Francesco DiMeco, Zhiyuan Xu, William Jeffrey Elias, Angelo Franzini, and Francesco Prada

Subjects
Cerebral ablation, radiofrequency, laser interstitial thermal therapy, high-intensity focused ultrasound, magnetic resonance-guided focused ultrasound, thermal lesioning, Medical technology, R855-855.5
Abstract

Background: Ablative therapies have been used for the treatment of neurological disorders for many years. They have been used both for creating therapeutic lesions within dysfunctional brain circuits and to destroy intracranial tumors and space-occupying masses. Despite the introduction of new effective drugs and neuromodulative techniques, which became more popular and subsequently caused brain ablation techniques to fall out favor, recent technological advances have led to the resurgence of lesioning with an improved safety profile. Currently, the four main ablative techniques that are used for ablative brain surgery are radiofrequency thermoablation, stereotactic radiosurgery, laser interstitial thermal therapy and magnetic resonance-guided focused ultrasound thermal ablation. Object: To review the physical principles underlying brain ablative therapies and to describe their use for neurological disorders. Methods: The literature regarding the neurosurgical applications of brain ablative therapies has been reviewed. Results: Ablative treatments have been used for several neurological disorders, including movement disorders, psychiatric disorders, chronic pain, drug-resistant epilepsy and brain tumors. Conclusions: There are several ongoing efforts to use novel ablative therapies directed towards the brain. The recent development of techniques that allow for precise targeting, accurate delivery of thermal doses and real-time visualization of induced tissue damage during the procedure have resulted in novel techniques for cerebral ablation such as magnetic resonance-guided focused ultrasound or laser interstitial thermal therapy. However, older techniques such as radiofrequency thermal ablation or stereotactic radiosurgery still have a pivotal role in the management of a variety of neurological disorders.

Published
2019
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42. Thalamic Local Field Potentials Are Related to Long-Term DBS Effects in Tourette Syndrome

Author

Sara Marceglia, Marco Prenassi, Tommaso F. Galbiati, Mauro Porta, Edvin Zekaj, Alberto Priori, and Domenico Servello

Subjects
Tourette syndrome, local field potentials, deep brain stimulation, tics, obsessive compulsive behaviors, Neurology. Diseases of the nervous system, RC346-429
Abstract

Background: Local field potential (LFP) recordings helped to clarify the pathophysiology of Tourette syndrome (TS) and to define new strategies for deep brain stimulation (DBS) treatment for refractory TS, based on the delivery of stimulation in accordance with changes in the electrical activity of the DBS target area. However, there is little evidence on the relationship between LFP pattern and DBS outcomes in TS.Objective: To investigate the relationship between LFP oscillations and DBS effects on tics and on obsessive compulsive behavior (OCB) comorbidities.Methods: We retrospectively analyzed clinical data and LFP recordings from 17 patients treated with DBS of the centromedian-parafascicular/ventralis oralis (CM-Pf/VO) complex, and followed for more several years after DBS in the treating center. In these patients, LFPs were recorded either in the acute setting (3–5 days after DBS electrode implant) or in the chronic setting (during impulse generator replacement surgery). LFP oscillations were correlated with the Yale Global Tic Severity Scale (YGTSS) and the Yale–Brown Obsessive–Compulsive Scale (Y-BOCS) collected at baseline (before DBS surgery), 1 year after DBS, and at the last follow-up available.Results: We found that, at baseline, in the acute setting, the power of the oscillations included in the 5–15-Hz band, previously identified as TS biomarker, is correlated with the pathophysiology of tics, being significantly correlated with total YGTSS before DBS (Spearman's ρ = 0.701, p = 0.011). The power in the 5–15-Hz band was also correlated with the improvement in Y-BOCS after 1 year of DBS (Spearman's ρ = −0.587, p = 0.045), thus suggesting a relationship with the DBS effects on OCB comorbidities.Conclusions: Our observations confirm that the low-frequency (5–15-Hz) band is a significant biomarker of TS, being related to the severity of tics and, also to the long-term response on OCBs. This represents a step toward both the understanding of the mechanisms underlying DBS effects in TS and the development of adaptive DBS strategies.

Published
2021
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43. Le attività di authority control in EDIT16: autori, titoli, editori/tipografi, marche e luoghi [Italian version presented at the International Conference]

Author

Leoncini, Claudia and Servello, Rosaria Maria

Subjects
I. Information treatment for information services
Abstract

The paper intends to present the experience gained in the field of authority control for the project Censimento nazionale delle edizioni italiane del XVI secolo (EDIT16) managed by ICCU with the cooperation of libraries, 1200 until today, in all the country. Within the projet ICCU is the scientific referent and coordinator, not only in the field of catalographic standards, compiling specific rules that have been integrated in the years, but also regarding the problems related to the choices and the accepted and variant forms of authors and printers. The necessity of setting from the centre some control lists for the recognition of the exemplars of the editions obliged to the priority of defining authority entries – in a stage in which the possibility of an automated management was far – using the traditional paper-based workflow.

Published
2003

44. Pre-dopa Deep Brain Stimulation: Is Early Deep Brain Stimulation Able to Modify the Natural Course of Parkinson’s Disease?

Author

Mauro Porta, Domenico Servello, Edvin Zekaj, Gabriel Gonzalez-Escamilla, and Sergiu Groppa

Subjects
Parkinson’s disease, deep brain stimulation, subthalamic nucleus, early deep brain stimulation, early intervention, Neurosciences. Biological psychiatry. Neuropsychiatry, RC321-571
Abstract

Deep brain stimulation (DBS) is an established therapy for the management of Parkinson’s disease (PD). However, DBS is indicated as the disease progresses and motor complications derived from pharmacological therapy arise. Here, we evaluate the potential of DBS prior to levodopa (L-Dopa) in improving quality of life (QoL), challenging the state of the art for DBS therapy. We present data on clinical manifestation, decision finding during early indication to DBS, and trajectories after DBS. We further discuss current paradigms for DBS and hypothesize on possible mechanisms. Six patients, between 50 and 67 years old, presenting at least 5 years of PD symptoms, and without L-Dopa therapy initiation, received subthalamic nucleus (STN) DBS implantation. In the six PD cases, indication for DBS was not driven by motor complications, as supported by current guidelines, but by relevant QoL impairment and patient’s reluctance to initiate L-Dopa treatment. All patients treated with STN-DBS prior to L-Dopa presented improvement in motor and non-motor symptoms and significant QoL improvement. All patients reduced the intake of dopamine agonists, and five are currently free from L-Dopa medication, with no reported adverse events. We introduce a multicenter observational study to investigate whether early DBS treatment may affect the natural course of PD. Early application of DBS instead of L-Dopa administration could have a pathophysiological basis and be prompted by a significant incline on QoL through disease progression; however, the clinical value of this proposed paradigm shift should be addressed in clinical trials aimed at modulating the natural course of PD.

Published
2020
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45. Caenorhabditis elegans processes sensory information to choose between freeloading and self-defense strategies

Author

Jodie A Schiffer, Francesco A Servello, William R Heath, Francis Raj Gandhi Amrit, Stephanie V Stumbur, Matthias Eder, Olivier MF Martin, Sean B Johnsen, Julian A Stanley, Hannah Tam, Sarah J Brennan, Natalie G McGowan, Abigail L Vogelaar, Yuyan Xu, William T Serkin, Arjumand Ghazi, Nicholas Stroustrup, and Javier Apfeld

Subjects
C. elegans, hydrogen peroxide, stress response, sensory perception, insulin signaling, TGFβ signaling, Medicine, Science, Biology (General), QH301-705.5
Abstract

Hydrogen peroxide is the preeminent chemical weapon that organisms use for combat. Individual cells rely on conserved defenses to prevent and repair peroxide-induced damage, but whether similar defenses might be coordinated across cells in animals remains poorly understood. Here, we identify a neuronal circuit in the nematode Caenorhabditis elegans that processes information perceived by two sensory neurons to control the induction of hydrogen peroxide defenses in the organism. We found that catalases produced by Escherichia coli, the nematode’s food source, can deplete hydrogen peroxide from the local environment and thereby protect the nematodes. In the presence of E. coli, the nematode’s neurons signal via TGFβ-insulin/IGF1 relay to target tissues to repress expression of catalases and other hydrogen peroxide defenses. This adaptive strategy is the first example of a multicellular organism modulating its defenses when it expects to freeload from the protection provided by molecularly orthologous defenses from another species.

Published
2020
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48. Balanced Activity between Kv3 and Nav Channels Determines Fast-Spiking in Mammalian Central Neurons

Author

Yuanzheng Gu, Dustin Servello, Zhi Han, Rupa R. Lalchandani, Jun B. Ding, Kun Huang, and Chen Gu

Subjects
Science
Abstract

Summary: Fast-spiking (FS) neurons can fire action potentials (APs) up to 1,000 Hz and play key roles in vital functions such as sound location, motor coordination, and cognition. Here we report that the concerted actions of Kv3 voltage-gated K+ (Kv) and Na+ (Nav) channels are sufficient and necessary for inducing and maintaining FS. Voltage-clamp analysis revealed a robust correlation between the Kv3/Nav current ratio and FS. Expressing Kv3 channels alone could convert ∼30%–60% slow-spiking (SS) neurons to FS in culture. In contrast, co-expression of either Nav1.2 or Nav1.6 together with Kv3.1 or Kv3.3, but not alone or with Kv1.2, converted SS to FS with 100% efficiency. Furthermore, RNA-sequencing-based genome-wide analysis revealed that the Kv3/Nav ratio and Kv3 expression levels strongly correlated with the maximal AP frequencies. Therefore, FS is established by the properly balanced activities of Kv3 and Nav channels and could be further fine-tuned by channel biophysical features and localization patterns. : Neuroscience; Molecular Neuroscience; Biophysics Subject Areas: Neuroscience, Molecular Neuroscience, Biophysics

Published
2018
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49. Custom-Made Poly(urethane) Coatings Improve the Mechanical Properties of Bioactive Glass Scaffolds Designed for Bone Tissue Engineering

Author

Monica Boffito, Lucia Servello, Marcela Arango-Ospina, Serena Miglietta, Martina Tortorici, Susanna Sartori, Gianluca Ciardelli, and Aldo R. Boccaccini

Subjects
bioactive glass, poly(urethane)s, dip-coating, replication method, bone tissue engineering, Organic chemistry, QD241-441
Abstract

The replication method is a widely used technique to produce bioactive glass (BG) scaffolds mimicking trabecular bone. However, these scaffolds usually exhibit poor mechanical reliability and fast degradation, which can be improved by coating them with a polymer. In this work, we proposed the use of custom-made poly(urethane)s (PURs) as coating materials for 45S5 Bioglass®-based scaffolds. In detail, BG scaffolds were dip-coated with two PURs differing in their soft segment (poly(ε-caprolactone) or poly(ε-caprolactone)/poly(ethylene glycol) 70/30 w/w) (PCL-PUR and PCL/PEG-PUR) or PCL (control). PUR-coated scaffolds exhibited biocompatibility, high porosity (ca. 91%), and improved mechanical properties compared to BG scaffolds (2–3 fold higher compressive strength). Interestingly, in the case of PCL-PUR, compressive strength significantly increased by coating BG scaffolds with an amount of polymer approx. 40% lower compared to PCL/PEG-PUR- and PCL-coated scaffolds. On the other hand, PEG presence within PCL/PEG-PUR resulted in a fast decrease in mechanical reliability in an aqueous environment. PURs represent promising coating materials for BG scaffolds, with the additional pros of being ad-hoc customized in their physico-chemical properties. Moreover, PUR-based coatings exhibited high adherence to the BG surface, probably because of the formation of hydrogen bonds between PUR N-H groups and BG surface functionalities, which were not formed when PCL was used.

Published
2021
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