Search

Your search keyword '"Serva, Saulius"' showing total 133 results
Start Over Author "Serva, Saulius"
133 results on '"Serva, Saulius"'

1. Diversity of RNA viruses in the cosmopolitan monoxenous trypanosomatid Leptomonas pyrrhocoris

Author

Macedo, Diego H., Grybchuk, Danyil, Režnarová, Jana, Votýpka, Jan, Klocek, Donnamae, Yurchenko, Tatiana, Ševčík, Jan, Magri, Alice, Dolinská, Michaela Urda, Záhonová, Kristína, Lukeš, Julius, Servienė, Elena, Jászayová, Alexandra, Serva, Saulius, Malysheva, Marina N., Frolov, Alexander O., Yurchenko, Vyacheslav, and Kostygov, Alexei Yu.

Published
2023
Full Text
View/download PDF

2. Profiles of Killer Systems and Volatile Organic Compounds of Rowanberry and Rosehip-Inhabiting Yeasts Substantiate Implications for Biocontrol.

Author

Vepštaitė-Monstavičė, Iglė, Lukša-Žebelovič, Juliana, Apšegaitė, Violeta, Mozūraitis, Raimondas, Lisicinas, Robertas, Stanevičienė, Ramunė, Blažytė-Čereškienė, Laima, Serva, Saulius, and Servienė, Elena

Subjects
VOLATILE organic compounds, DOUBLE-stranded RNA, GRAPE juice, FOOD preservation, METABOLITES, BERRIES
Abstract

Yeasts produce numerous antimicrobial agents such as killer toxins, volatile organic compounds (VOCs), and other secondary metabolites, establishing themselves in developing natural and sustainable biocontrol strategies for agriculture and food preservation. This study addressed the biocontrol potential of yeasts, isolated from spontaneous fermentations of rosehips (Rosa canina L.) and rowanberries (Sorbus aucuparia L.), focusing on their killer phenotypes and VOCs production. Yeasts were isolated using spontaneous fermentations with Hanseniaspora uvarum and Metschnikowia pulcherrima identified as the dominant species, comprising approximately 70% of the yeast population. Among 163 isolated strains, 20% demonstrated killing activity, with Saccharomyces cerevisiae exhibiting the strongest killing efficiency, as well as Pichia anomala and M. pulcherrima showing broad-spectrum antagonistic activity. This study identified dsRNA-encoded killer phenotypes in S. cerevisiae, S. paradoxus, and Torulaspora delbrueckii, revealing multiple distinct killer toxin types. The biocontrol potential of wild berry-inhabiting yeasts was demonstrated in a real food system, grape juice, where the S. cerevisiae K2-type killer strain significantly reduced fungal contaminants. The selected H. uvarum, M. pulcherrima, S. cerevisiae, and S. paradoxus yeast strains representing both berries were applied for VOC analysis and identification by gas chromatography-linked mass spectrometry. It was revealed that the patterns of emitted volatiles are yeast species-specific. Statistically significant differences between the individual VOCs were observed among killing phenotype-possessing vs. non-killer S. paradoxus yeasts, thus revealing the involvement of killer systems in multi-level biocontrol enablement. The performed studies deepen our understanding of potential yeast biocontrol mechanisms, highlight the importance of produced antimicrobials and volatiles in ensuring antagonistic efficacy, and prove the relevance of isolated biocontrol yeasts for improving food safety. [ABSTRACT FROM AUTHOR]

Published
2025
Full Text
View/download PDF

3. Distinct microbial communities associated with health‐relevant wild berries.

Author

Vepštaitė‐Monstavičė, Iglė, Lukša, Juliana, Strazdaitė‐Žielienė, Živilė, Serva, Saulius, and Servienė, Elena

Subjects
BACTERIAL communities, MICROBIAL communities, FOOD safety, PLANT health, SEQUENCE analysis
Abstract

Lingonberries (Vaccinium vitis‐idaea L.), rowanberries (Sorbus aucuparia L.) and rosehips (Rosa canina L.) positively affect human health due to their healing properties, determined by a high content of bioactive compounds. The consumption of unprocessed wild berries is relevant and encouraged, making their in‐depth microbiological characterization essential for food safety. This study presents the first high‐throughput sequencing analysis of bacterial and fungal communities distributed on the surface of lingonberries, rowanberries and rosehips. Significant plant‐defined differences in the taxonomic composition of prokaryotic and eukaryotic microbiota were observed. The bacterial community on rosehips was shown to be prevalent by Enterobacteriaceae, lingonberries by Methylobacteriaceae and rowanberries by Sphingomonadaceae representatives. Among the fungal microbiota, Dothioraceae dominated on rosehips and Exobasidiaceae on lingonberries; meanwhile, rowanberries were inhabited by a similar level of a broad spectrum of fungal families. Cultivable yeast profiling revealed that lingonberries were distinguished by the lowest amount and most distinct yeast populations. Potentially pathogenic to humans or plants, as well as beneficial and relevant biocontrol microorganisms, were identified on tested berries. The combination of metagenomics and a cultivation‐based approach highlighted the wild berries‐associated microbial communities and contributed to uncovering their potential in plant health, food and human safety. [ABSTRACT FROM AUTHOR]

Published
2024
Full Text
View/download PDF

8. Viral discovery and diversity in trypanosomatid protozoa with a focus on relatives of the human parasite Leishmania

Author

Grybchuk, Danyil, Akopyants, Natalia S., Kostygov, Alexei Y., Konovalovas, Aleksandras, Lye, Lon-Fye, Dobson, Deborah E., Zangger, Haroun, Fasel, Nicolas, Butenko, Anzhelika, Frolov, Alexander O., Votýpka, Jan, d’Avila-Levy, Claudia M., Kulich, Pavel, Moravcová, Jana, Plevka, Pavel, Rogozin, Igor B., Serva, Saulius, Lukeš, Julius, Beverley, Stephen M., and Yurchenko, Vyacheslav

Published
2018

10. Diversity of RNA viruses in the cosmopolitan monoxenous trypanosomatid Leptomonas pyrrhocoris

Author

Fagundes Macedo, Diego, Grybchuk, Danyil, Reznarova, Jana, Votypka, Jan, Klocek, Donnamae, Yurchenko, Tatiana, Sevcik, Jan, Magri, Alice, Dolinska, Michaela Urda, Zahonova, Kristina, Lukes, Julius, Serviene, Elena, Jaszayova, Alexandra, Serva, Saulius, Malysheva, Marina N., Frolov, Alexander O., Yurchenko, Vyacheslav, Kostygov, Alexei Yu., Fagundes Macedo, Diego, Grybchuk, Danyil, Reznarova, Jana, Votypka, Jan, Klocek, Donnamae, Yurchenko, Tatiana, Sevcik, Jan, Magri, Alice, Dolinska, Michaela Urda, Zahonova, Kristina, Lukes, Julius, Serviene, Elena, Jaszayova, Alexandra, Serva, Saulius, Malysheva, Marina N., Frolov, Alexander O., Yurchenko, Vyacheslav, and Kostygov, Alexei Yu.

Abstract

Background Trypanosomatids are parasitic flagellates well known because of some representatives infecting humans, domestic animals, and cultural plants. Many trypanosomatid species bear RNA viruses, which, in the case of human pathogens Leishmania spp., influence the course of the disease. One of the close relatives of leishmaniae, Leptomonas pyrrhocoris, has been previously shown to harbor viruses of the groups not documented in other trypanosomatids. At the same time, this species has a worldwide distribution and high prevalence in the natural populations of its cosmopolitan firebug host. It therefore represents an attractive model to study the diversity of RNA viruses.Results We surveyed 106 axenic cultures of L. pyrrhocoris and found that 64 (60%) of these displayed 2-12 double-stranded RNA fragments. The analysis of next-generation sequencing data revealed four viral groups with seven species, of which up to five were simultaneously detected in a single trypanosomatid isolate. Only two of these species, a tombus-like virus and an Ostravirus, were earlier documented in L. pyrrhocoris. In addition, there were four new species of Leishbuviridae, the family encompassing trypanosomatid-specific viruses, and a new species of Qinviridae, the family previously known only from metatranscriptomes of invertebrates. Currently, this is the only qinvirus with an unambiguously determined host. Our phylogenetic inferences suggest reassortment in the tombus-like virus owing to the interaction of different trypanosomatid strains. Two of the new Leishbuviridae members branch early on the phylogenetic tree of this family and display intermediate stages of genomic segment reduction between insect Phenuiviridae and crown Leishbuviridae.Conclusions The unprecedented wide range of viruses in one protist species and the simultaneous presence of up to five viral species in a single Leptomonas pyrrhocoris isolate indicate the uniqueness of this flagellate. This is likely determined by th

Published
2023
Full Text
View/download PDF

13. Elimination of LRVs Elicits Different Responses in Leishmania spp.

Author

Saura, Andreu, primary, Zakharova, Alexandra, additional, Klocek, Donnamae, additional, Gerasimov, Evgeny S., additional, Butenko, Anzhelika, additional, Macedo, Diego H., additional, Servienė, Elena, additional, Zagirova, Diana, additional, Meshcheryakova, Anastasia, additional, Rogozin, Igor B., additional, Serva, Saulius, additional, Kostygov, Alexei Yu., additional, and Yurchenko, Vyacheslav, additional

Published
2022
Full Text
View/download PDF

14. Adaptive response of saccharomyces hosts to totiviridae L-A dsRNA viruses Is achieved through intrinsically balanced action of targeted transcription factors

Author

Universidad de Sevilla. Departamento de Genética, Fondo Social Europeo (FSO), Ravoitytė, Bazilė, Lukša, Juliana, Wellinger, Ralf Erik, Serva, Saulius, Servienė, Elena, Universidad de Sevilla. Departamento de Genética, Fondo Social Europeo (FSO), Ravoitytė, Bazilė, Lukša, Juliana, Wellinger, Ralf Erik, Serva, Saulius, and Servienė, Elena

Abstract

Totiviridae L-A virus is a widespread yeast dsRNA virus. The persistence of the L-A virus alone appears to be symptomless, but the concomitant presence of a satellite M virus provides a killer trait for the host cell. The presence of L-A dsRNA is common in laboratory, industrial, and wild yeasts, but little is known about the impact of the L-A virus on the host’s gene expression. In this work, based on high-throughput RNA sequencing data analysis, the impact of the L-A virus on whole-genome expression in three different Saccharomyces paradoxus and S. cerevisiae host strains was analyzed. In the presence of the L-A virus, moderate alterations in gene expression were detected, with the least impact on respiration-deficient cells. Remarkably, the transcriptional adaptation of essential genes was limited to genes involved in ribosome biogenesis. Transcriptional responses to L-A maintenance were, nevertheless, similar to those induced upon stress or nutrient availability. Based on these data, we further dissected yeast transcriptional regulators that, in turn, modulate the cellular L-A dsRNA levels. Our findings point to totivirus-driven fine-tuning of the transcriptional landscape in yeasts and uncover signaling pathways employed by dsRNA viruses to establish the stable, yet allegedly profitless, viral infection of fungi.

Published
2022

15. Adaptive Response of Saccharomyces Hosts to Totiviridae L-A dsRNA Viruses Is Achieved through Intrinsically Balanced Action of Targeted Transcription Factors

Author

European Commission, Ravoitytė, Bazilė, Lukša, Juliana, Wellinger, Ralf Erik, Serva, Saulius, Servienė, Elena, European Commission, Ravoitytė, Bazilė, Lukša, Juliana, Wellinger, Ralf Erik, Serva, Saulius, and Servienė, Elena

Abstract

Totiviridae L-A virus is a widespread yeast dsRNA virus. The persistence of the L-A virus alone appears to be symptomless, but the concomitant presence of a satellite M virus provides a killer trait for the host cell. The presence of L-A dsRNA is common in laboratory, industrial, and wild yeasts, but little is known about the impact of the L-A virus on the host’s gene expression. In this work, based on high-throughput RNA sequencing data analysis, the impact of the L-A virus on whole-genome expression in three different Saccharomyces paradoxus and S. cerevisiae host strains was analyzed. In the presence of the L-A virus, moderate alterations in gene expression were detected, with the least impact on respiration-deficient cells. Remarkably, the transcriptional adaptation of essential genes was limited to genes involved in ribosome biogenesis. Transcriptional responses to L-A maintenance were, nevertheless, similar to those induced upon stress or nutrient availability. Based on these data, we further dissected yeast transcriptional regulators that, in turn, modulate the cellular L-A dsRNA levels. Our findings point to totivirus-driven fine-tuning of the transcriptional landscape in yeasts and uncover signaling pathways employed by dsRNA viruses to establish the stable, yet allegedly profitless, viral infection of fungi.

Published
2022

22. Contributory presentations/posters

Author

Gries, A., Singh, Balwinder, Nakazawal, Chicko, Genest, D., Getzoff, E. D., Matsuo, H., Kaur, Harpreet, Borst, J. W., Chadha, K. C., Tingyun, Kuang, Jagannadham, M. V., Leijon, Mikael, Sato, S., Bhakuni, Vlnod, Vijayan, M., Surolia, A., Suguna, K., Manoj, N., Srinivas, V. R., Ravishankar, R., Laggner, P., Prassl, R., Schwarzenbacher, R., Zeth, K., Kostner, G. M., Taylor, Susan S., Xuong, Nguyen-huu, Akamine, Pearl, Sagar, Bidva M., Saikrishnan, K., Purnapatre, K., Handa, P., Roy, S., Varshney, U., Biswal, B. K., Sukumar, N., Rao, J. K. Mohana, Johnson, A., Pattabhi, Vasantha, Murthy, M. R. N., Krishna, Sri S., Savithri, H. S., Sastri, Mira, Hosur, M. V., Pillai, Bindu, Kannan, K. K., Kumar, Mukesh, Patwardhan, Swati, Padmanabhaa, B., Sasaki-Sugio, S., Matsuzaki, T., Nukaga, M., Singh, T. P., Sharma, A. K., Srinivasan, A., Khan, J. A., Paramasivam, M., Kumar, P., Karthikevan, S., Sharma, S., Yadav, S., Srintvasan, A., Alam, Neelima, Gourinath, S., Kaur, Punit, Chandra, Vikas, Betzel, Ch., Ghosh, S., Bera, A. K., Pal, A. K., Baneriee, Asok, Mukhopadhyay, B. P., Bhattacharya, S., Chakraborty, S., Haldar, U., Dey, I., Solovicova, Adriana, Sevcik, Jozef, Sekar, K., Sundaralingam, M., Genov, N., Liang, Dong-cai, Zhang, Ji-ping, Jiang, Tao, Chang, Wen-rui, Blommers, Marcel, Jahnke, Wolfgang, Hosur, R. V., Panchal, S. C., Pillay, Bindu, Jaganathan, N. R., Mathur, Puniti, Srivatsun, S., Joshi, Ratan Mani, Chauhan, V. S., Govil, Girjesh, Atreya, H. S., Sahu, S. C., Quinjou, Éric, Adjadj, Elisabeth, Mispelter, Joël, Izadi-Pruneyre, Nadia, Blouquit, Yves, Heyd, Bernadette, Lerat, Guilhem, Desmadreil, Michel, Milnard, Philippe, Lin, Y., Rao, B. D. Nageswara, Raghunathan, Vidva, Chau, Mei H., Coutinho, Evans, Pesais, Prashant, Srivastava, Sudha, Saran, Anil, Srikrishnan, Thamarapu, Lijima, Herbert, Gesme, Jayson, Sapico, Leizl F., Paxton, Raymond, Grace, C. R., Nagenagowda, G., Lynn, A. M., Cowsik, Sudha M., Govil, G., Sahu, Sarata C., Bhattacharya, A., Chauhan, S., Kumar, Anil, Zuiderweg, Erik R. P., Pellecchia, Maurizio, Nitta, Katsutoshi, Ohnishi, Atsushi, Kawano, Keiichi, Hikichi, Kunio, Fujitani, Naoki, Ohkubo, Tadayasu, Aizawa, Tomoyasu, Kumaki, Yasuhiro, Hayakawa, Yoichi, Parvathy, Rani V., Kini, R. M., Nakagawa, Astushi, Tanaka, Isao, Demura, Makoto, Yao, Min, Koshiba, Takumi, Kobashigawa, Yoshihiro, Kuwajima, Kunihiro, Linge, Jens, Nilges, Michael, Donoghue, Seán O., Chakshusmathi, G., Ratnaparkhi, Girish S., Madhu, P. K., Varadarajan, R., Tetreau, C., Tourbez, M., Lavalette, D., Bulone, D., Manno, M., Emanuele, A., Palma-Vittorelli, M. B., Palma, M. U., Vaiana, S. M., Martorana, V., Biagio, P. L. San, Chang, D. K., Cheng, S. F., Yang, S. H., Francis, S., Trivedi, V. D., Chien, W. J., Manstein, Dietmar J., Batra, Renn, Geeves, Michael A., Geller, Maciej, Trvlska, Joanna, Grochowski, Pawel, Lesyng, B., Ginalski, K., Grochowski, P., Lavalette, P., Blouquit, Y., Roccatano, D., Berendsen, H. J. C., Amadei, A., Nola, Di A., Ho, Bosco, Curmi, P. M. G., Berry, H., Pelta, J., Pauthe, E., Lairez, D., Srinivasan, M., Sahi, Shakti, Kothekar, V., Madhusudnan, Kartha S., Nandel, Fateh S., Jain, D. V. S., Berendsen, Herman J. C., Feenstra, Anton K., Tama, F., Sanejouand, Y.-H., Go, N., Sharma, Deepak, Pasha, Santosh, Sharma, Sunita, Brahmachari, Samir K., Makker, Jyoti, Viiavaraghavan, R., Kumar, S., Dey, Sharmisllia, Krishnamoorthy, G., Lakshmikanth, G. S., Zaitseva, E. M., Mazhul, V. M., Kierdaszuk, Borys, Widengren, J., Rigler, R., Terry, B., Mets, Ü., Swaminathan, R., Yathindra, N., Thamotharan, S., Chosrowjan, H., Mataga, N., Shibata, Y., Morisima, I., Xiao, Ming, Selvin, Paul, Chakraharty, Tania, Cooke, Roger, Faraone, A., Branca, C., Maisano, G., Migliardo, P., Magazù, S., Villari, V., Behere, Digambar V., Deva, Sharique Zahida Waheed M., Vallone, B., Savino, C., Travaglini-Allocatelli, C., Cutruzzolà, F., Brunori, M., Gibson, Q. H., Mazumdar, Shyamalava, Mitra, Samaresh, Prasad, Swati, Soto, P., Fayad, R., Tyulkova, N. A., Sukovataya, I. E., Mamedov, Sh. V., Aksakal, B., Canturk, M., Aktas, B., Yilgin, R., Bogutska, K. I., Miroshnichenko, N. S., Wein, A. J., Hypolite, J. A., DiSanto, M., Chacko, S., Zheng, Y-M., Antosiewicz, J., Wojciechowski, M., Grycuk, T., Di Nola, Alfredo, Ceruso, Marc A., Chatterjee, Bishnu P., Bandvopadhvay, Subhasis, Choudhury, Devapriva, Khight, Stefan, Thompson, Andrew, Stojanoff, Vivian, Pinkner, Jerome, Hultgren, Scott, Flatters, Delphine, Goodfellow, Julia, Takazawatt, Fumi, Kanehisa, Minoru, Sasai, Masaki, Nakamura, Hironori, Wang, Bao Han, Pan, xin Min, Zheng, Yuan, Wang, Zhi Xin, Ahmad, Atta, Kulkarni, Sangeeta, Prakash, Koodathingal, Prajapati, Shashi, Surin, Alexey, Kihara, Hiroshi, Yang, Li, Matsumoto, Tomoharu, Nakagawa, Yuki, Semisotnov, Gennady V., Kimura, Kazumoto, Amemiya, Yoshiyuki, Tayyab, Saad, Muzammil, Salman, Kumar, Yogesh, Bhakuni, Vinod, Sundd, Monica, Kundu, Suman, Jagannadham, Medicherla V., Chandani, Bina, Warrier, Deepti, Sinha, Lalankumar, Dhar, Ruby, Mehrotra, Sonam, Khandelwal, Purnima, Seth, Subhendu, Gidwani, Arun, Prabha, Ratna C., Sasidhar, Y. U., Madhusudan, K. P., Nishikawa, Ken, Kinjo, Akira R., Varadarajan, Raghavan, Chakravarty, Suvobrata, Van Dael, H., Noyelle, K., Joniau, M., Haezebrouck, P., Jha, Indra Brata, Bhat, Rajiv, Dash, Sheffali, Mohanty, Prasanna, Bandyopadhyay, A. K., Sonawat, H. M., Rao, Ch. Mohan, Datta, Siddhartha, Raman, B., Rajaraman, K., Ramakrishna, T., Pande, A., Benedek, G., King, J., Betts, S., Pande, J., Asherie, N., Ogun, O., Kalacheva, G. S., Sokolova, I. V., Mitaku, Shigeki, Sonoyama, Masashi, Taira, Kunihiro, Yokoyama, Yasunori, Sasakil, Takanori, Kamo, Naoki, Mukai, Yuri, Dalal, Seema, Regan, Lynne, Mituku, Shigeki, Kumar, Devesh, Roychoudhury, Mihir, Lőrinczv, Dénes, Könczöl, Franciska, Farkas, László, Belagyi, Joseph, Schick, Christoph, Thomson, Christy A., Ananthanarayanan, Vettai S., Alirzayeva, E. G., Baba-Zade, S. N., Sarai, A., Kono, H., Uedaira, H., An, J., Gromiha, Michael M., Oobatake, M., Yutani, Katsuhide, Takano, Kazufumi, Yamagata, Yuriko, Jas, Gouri S., Hofrichter, James, Muñoz, Victor, Eaton, William A., Penoyar, Jonathan, Lo Verde, Philip T., Bódi, Á., Venekei, I., Kardos, J., Gráf, L., Závodszky, P., Szilágyi, András, Závodszky, Péter, Woolfson, D. N., Walshaw, J., Allan, R. D., Funahashi, Jun, Gupta, Savan, Di Nola, A., Mangoni, M., Roccatano, P., Ramachandraiah, Gosu, Chandra, Nagasuma R., Ciani, Barbara, Woolfson, Derek N., Nair, Usha B., Salunke, Dinakar M., Kaur, Kanwal J., Swaminathan, Chittoor P., Surolia, Avadhesha, Pramanik, A., Jörnvall, H., Nygren, P.-Å., Jonasson, P., Ståhl, S., Johansson, B.-L., Kratz, G., Wahren, J., Ekberg, K., Uhlén, M., Jansson, O. T., Uhlén, S., Misselwitz, Rolf, Welfle, Heinz, Welfle, Karin, Höhne, Wolfgang, Kurganov, B. I., Mitskevich, L. G., Fedurkina, N. V., Jarori, Gotam K., Maity, Haripada, Guharay, J., Sengupta, P. K., Sengupta, B., Sridevi, K., Kasturi, S. R., Gupta, S. P., Agarwal, Gunjan, Briehl, Robin W., Kwong, Suzanne, Tyulkova, N A., Ismailova, O. I., Parola, A. H., Yayon, A., Hariharan, C., Pines, D., Pines, E., Zamai, M., Cohen-Luria, R., Woolfeon, D. N., Spooner, G. A., Padya, M. J., Bharadwaj, D. K., Bakshi, Panchan, Jagannathan, N. R., Sharma, U., Srivastava, N., Barthwal, R., Matsuda, Keiko, Nishioka, Takaaki, Go, Nobuhiro, Urata, S., Aita, T., Husimi, Y., Majumder, Mainak, Subirana, Juan A., Malinina, Lucy, Abrescia, Nicola G. A., Aymami, Juan, Coll, Miquel, Eritxa, Ramón, Premraj, B. J., Thenmalarchelvi, R., Gautham, N., Kumar, Satheesh P., Kan, Lou-Sing, Hou, Ming, Lin, Shwu-Bin, Roy, Kanal B., Sana, Tapas, Bruant, N., Flatters, D., Lavery, R., Sklenar, Heinz, Rons, Remo, Lavery, Richard, Thakur, Ashoke Ranjan, Kundu, Sudip, Bandyopadhyay, Debashree, Bhattacharyya, Dhananjay, Majumdar, Rabi, Barceló, F., Portugal, J., Rao, B. J., Ramanathan, Sunita, Gliosli, Mahua, Varshney, Umesh, Kumar, Vinay N., Pataskar, Shashank S., Sarojini, R., Selvasekarapandian, S., Kolandaivel, P., Sukumar, S., Kolmdaivel, P., Maiti, Motilal, Das, Suman, Sen, Anjana, Xodo, Luigi, Suraci, Chiara, Del Terra, Elisa, Quadrifoglio, Franco, Diviacco, Silvia, Ray, Arghya, Rao, Basuthkar J., Karthikeyan, G., Chary, Kandala V. R., Mujeeb, Anwer, James, Thomas L., Bogdanov, A., Zanina, A., Haya, E. E. F., Kasyanenko, N., Cornélio, M. L., Bugs, M. R., Tolstorukov, Ye. M., Sanval, Nitish K., Tiwari, S. N., Sanyal, Nitish K., Choudhury, Mihir Roy, Patel, P. K., Bhavesh, Neel S., Gabrielian, Anna, Rigler, Rudolf, Edman, Lars, Wennmalm, Stefan, Constantinescu, B., Gazdaru, D., Radulcscu, I., Radu, L., Wärmländer, Sebastian, Aoki, Setsuyuki, Ishiura, Masahiro, Kondo, Takao, Pashinskaya, V. A., Kosevich, M. V., Shelkovsky, V. S., Blagoy, Yu. P., Wang, Ji-hua, Malathi, R., Chandrasekhar, K., Kandimalla, E. R., Agrawal, S., Rastogi, V. K., Palafox, Alcolea M., Singh, Chatar, Beniaminov, A. D., Minyat, E. E., Zdobnov, E. M., Ulyanov, N. B., Bondarenko, S. A., Ivanov, V. I., Singh, J. S., Tewari, Ravindra, Sonawane, Kailas D., Grosjean, Henri, Sonavane, Uddhavesh B., Morin, Annie, Doherty, Elizabeth A., Doudna, Jennifer A., Tochio, H., Shirakawa, M., Kyogoku, Y., Das, Achintya, Javaram, B., Kalra, Parul, Shukla, Piyush, Dixit, Surjit B., Beveridge, David L., McConnell, Kevin, Davidson, B. E., Chan, R. Y. S., Sawyer, W. H., Eccelston, J. F., Yan, Yuling, Norden, Bengt, Tuite, Eimer, Nielsen, Peter, Takahashi, Masayuki, Ghosh, Anirban, Bansal, Manju, Pingoud, Alfred, Christ, Frauke, Thole, Hubert, Pingoud, Vera, Wende, Wolfgang, Luthra, Pratibha Mehta, Chandra, Ramesh, Sen, Ranjan, Weisberg, Robert, King, Rodney, Gobets, Bas, van Amerongen, Herbert, van Stokkum, Ivo H. M., Larsen, Olaf F. A., van Grondelle, Rienk, Hilbers, Cornelis W., Heus, Hans A., Berends, Jos, Sngrvan, H E., Khudaverdian, N. V., Babayan, Yu. S., Pichierri, F., Gromiha, M., Prabakaran, P., Aida, M., Sayano, K., Merkienė, Eglė, Vilkaitis, Giedrius, Klimašauskas, Saulius, Serva, Saulius, Weinhold, Elmar, Bandiera, Antonella, Marsich, Eleonora, Manzini, Giorgio, Potikyan, G., Arakelyan, V., Babayan, Yu., Ninaber, Alex, Goodfellow, Julia M., Ohta, Shigeru, Ito, Yoichiro, Husimi, Yuzuru, Usukura, J., Aiba, H., Tagami, H., Nunes, Elia, Suarez, Mougli, Candreva, Carmen E., Keszenman, Deborah, Thyberg, Per, Földes-Papp, Zeno, Joshi, Amita, Singh, Dinesh, Rajeswari, M. R., Amenitsch, H., Pregetter, M., Chapman, J., Mishra, K. P., Pandev, B. N., Tonevitsky, A. G., Pohl, E. E., Agapov, I. I., Sun, J., Pohl, P., Dennison, S. M., Gorbeako, G. P., Dynbko, T. S., Mishra, A. K., Pappavee, N., Luis, Loura, Rodrigo, Almeida, Manuel, Prieto, Gendel, Ya. L., Kleszczyńska, H., Kuczera, J., Przestalski, S., Kral, T., Chernitsky, E. A., Senkovich, O. A., Rosin, V. V., Gasanov, R. A., Allakhverdieva, Y. M., Papageorgiou, G. C., Savopol, Tudor, Apetrei, Calin, Balea, Marius, Cucu, D., Mihailescu, D., Ramanathan, K. V., Bačić, Goran, Genest, Monique, Sajot, Nicolas, Garnier, Norbert, Crouzy, Serge, Zsiros, O., Várkonyi, Z. S., Combos, Z., Farkas, T., Cribier, Sophie, de Paula, F., Fraceto, I. F., Schreier, S., Spisni, A., Sevšek, F., Žekš, B., Gomišček, G., Svetina, S., Arrigler, V., Hotani, Hirokazu, Nomura, Fumimasa, Takiguchi, Kingo, Nagata, Miki, Panicker, Lata, Parvathanathan, P. S., Hotani, H., Takiguchi, K., Ishino, A., Saitoh, A., Afonin, S., Takahashi, A., Takizawa, T., Nakato, Y., Marathe, Dipti, Jørgensen, Kent, Chattopadhyay, Amitabha, Rukmini, R., Rawat, Satinder S., Pečar, S., Štrancar, J., Šentiurc, M., Stolič, Z., Filipin, K., Biswas, S. C., Samanta, Anunay, Sana, Satyen, Kinoshita, Koji, Yamazaki, Masahito, Ohki, Kazuo, Goto, Akira, Kiuchi, Tai, Kumeta, Takaaki, Ohba, Tetsuhiko, Sugar, I. P., Thompson, K. K., Biltonen, R. L., Thompson, T. E., Ichinose, H., Suezaki, Y., Akivama, M., Matuoka, S., Tsuchihashi, K., Gasa, S., Pike, H. M., Mattjus, P., Brown, R. E., Molotkovsky, J. G., Arora, Ashish, Kleinschmidt, Jörg H., Tamm, Lukas K., Kruglyakova, K. E., Luneva, O. G., Fedin, V. A., Kuptsoya, O. S., Visser, A. J. W. G., Visser, N. V., Dyubko, T. S., Ogihara, Toshihiko, Mishima, Kiyoshi, Shvaleva, A. L., Radenović, Č. N., Jeremić, M. G., Radenović, N. Č., Minić, P. M., Salakhutdinov, B. A., Aripov, T. F., Tadjibaeva, E. T., Zamaraeva, M. V., Vagina, O. N., Basak, A. K., Cole, A., Naylor, C., Poppofl, M., Titball, R., Naylor, C. E., Moss, D. S., Eaton, J. T., Justin, N., Titball, R. W., Nomura, F., Nagata, M., Ishjkawa, S., Takahashi, S., Obuchi, Kaoru, Staudegger, Erich, Lohner, Karl, Kriechbaum, Manfred, Waring, Alan J., Lehrer, Robert I., Mayer, Bernd, Köhler, Gottfried, Gangl, Susanne, Shobini, J., Hu, B., Lortz, B., Sackmann, E., Guttenberg, Z., Antonovich, A. N., Slobozhanina, E. I., Lukyanenko, L. M., Kozlova, N. M., Krylov, Andrey V., Kotova, Elena A., Antonenko, Yuri N., Yaroslavov, Alexander A., Ghosh, Subhendu, Bera, Amal K., Das, Sudipto, Urbánková, Eva, Freeman, Karl, Jelokhani-Niaraki, Masood, Jezek, Petr, Usmanov, P. B., Tonkikh, A. K., Ongarbaev, A., Pohl, Peter, Saparov, Sapar M., Harikumar, P., Reeves, J. P., Sikdar, S. K., Rao, S., Ghatpande, A. S., Corsso, C., Varanda, W. A., ElHamel, C., Dé, E., Molle, G., Saint, N., Varshney, Anurae, Mathew, M. K., Isacoff, E. Y., Loots, E., Kasai, Michiki, Yamaguchi, Naohiro, Ghosh, Paramita, Tigyi, Joseph, Miledi, Ricardo, Tigyi, Gabor, Liliom, Karoly, Djurisic, Maja R., Andjus, Pavle R., Shrivastava, Indira H., Sansom, M. S. P., Barrias, C., Oliveira, P. F., Lopes, I. A., Mauricio, A. C., Fedorovich, S. V., Konev, S. V., Sholukh, M. V., Chubanov, V. S., Klevets, M., Fedirko, N., Shvinka, N., Manko, V., Prabhananda, B. S., Kombrabail, Mamata H., Aravamudhan, S., Venegas-Cotero, Berenice, Blake, Ivan Ortega, Zhou, Han-qing, Hu, Xiao-jian, Zhang, Zhi-hong, Feng, Hang-fang, Cheng, Wei-ying, Zalyvsky, I. A., Dubitsky, L. O., Vovkanvch, L. S., Savio-Galimberti, E., Ponce-Homos, J. E., Bonazzola, P., Capurro, Claudia, Parisi, Mario, Toriano, Roxana, Thomas, David D., Ready, Laxma G., Jones, Larry R., Tashmukhamedov, B. A., Sagdullaev, B. T., Heitzmann, D., Bleich, M., Warth, R., Ferreira, H. G., Ferreira, K. T. G., Greger, R., Parola, Abraham H., Alfahel, Essa, Zagoory, Orna, Priel, Zvi, Hama-Inaba, H., Ohyama, H., Hayata, I., Choi, K., Haginoya, K., Mori, M., Wang, R., Yukawa, O., Nakajima, T., Joshi, Nanda B., Kannurpatti, Sridhar K., Sinha, Mau, Joshi, Preeti G., Bei, Ling, Hu, Tianhui, Shen, Xun, Knetsch, Menno L. W., Schäfers, Nicole, Sandblom, John, Galvanovskis, Juris, Kovacs, Eugenia, Dinu, Alexandra, Pologea-Moraru, Roxana, Sanghvi, S. H., Jazbinšek, V., Tronteli, Z., Thiel, G., Wübeller, G., Müller, W., Brumen, Milan, Fajmut, Leš, Marhl, Marko, Volotovski, I. D., Sokolovski, S. G., Knight, M. R., Chalyi, Alexander V., Vasilʼev, Alexei N., Sharma, P., Pant, H. C., Sharma, M., Amin, N. D., Albers, R. W., Steinbach, P. J., Barchir, J., Balasubramanyam, M., Gardner, J. P., Condrescu, M., Pilarczyk, Gotz, Greulich, K. O., Monajembashi, Shamci, El-Awadi, A. I., El-Refaei, F. M., Talaat, M. M., Ali, F. M., Zahradniková, Alexandra, Tahradník, Ivan, Pavelková, Jana, Zhorov, Boris S., Ananthanaravanan, Vettai S., Weiss, D. G., Martin, D., Gornik, E., Neu, E., Michailov, Ch. M., Welscher, U., Seidenbusch, W., Jellali, A., Pattnaik, B. R., Hicks, D., Dreyfus, H., Sahel, J., Picaud, S., Forster, V., Wang, Hong-Wei, Sui, Sen-fang, Luther, Pradeep K., Morris, Ed, Barry, John, Squire, John, Sundari, Sivakama C., Balasubramanian, D., Christlet, Hema Thanka T., Veluraia, K., Suresh, Xavier M., Laretta-Garde, V., Krilov, Dubravka, Herak, Janko N., Stojanović, Nataša, Ferrone, Frank A., Ivanova, Maria, Jasuja, Ravi, Mirchev, Rossen, Stopar, David, Wolfs, Cor J. A. M., Hemminga, Marcus A., Spruijt, Ruud B., Arcovito, G., De Spirito, M., Frank, Joachim, Heagle, Amy B., Grassucci, Robert, Penczek, Pawel, Agrawal, Rajendra K., Sharma, Manjuli R., Wagenknecht, Terence, Jeyakumar, Loice H., Fleischer, Sidney, Knupp, Carlo, Squire, John M., Ezra, Eric, Munro, Peter M. G., Kitazawa, Hidefumi, Ichihara, Koji, Itoh, Tomohiko J., Iguchi, Yusuke, Pifat, Greta, Kveder, Marina, Pečar, Slavko, Schara, Milan, Nair, Deepak, Singh, Kavita, Rao, Kanury V. S., Sundaravadivel, B., Jain, Deepti, Kaur, Kanwaljeet, Salunke, D. M., Goel, Manisha, Kovalenko, E. I., Semenkova, G. N., Cherenkevich, S. N., Loganathan, D., Lakshmanan, T., Sriram, D., Srinivasan, S., Lebrón, J. A., Bjorkman, P. J., Ramalingam, T. S., Singh, A. K., Gayatri, T. N., Bisch, Paulo M., Caffarena, Ernesto R., Grigera, Raul J., Fromherz, P., Kiessling, V., Suresh, C. G., Rao, K. N., Khan, M. I., Gaikwad, S. M., Elanthiraiyan, M., Kaliannan, P., Payne, J., Chadha, K., Ambrus, J. L., Nair, M. P. N., Nair, Madhavan P. N., Hewitt, R., Schwartz, S. A., Mahajan, S., Macherel, D., Bourguignon, J., Neuburger, M., Douce, R., Cohen-Addad, C., Faure, M., Ober, R., Sieker, L., Gurumurthy, D. S., Velmurugan, S., Lobo, Z., Phadke, Ratna S., Desai, Prashant, Alieva, D. R., Guseinova, I. M., Zulfugarov, I. S., Aliev, J. A., Ismayilov, M. A., Novruzova, S. N., Savchenko, T. V., Suleimanov, Yu. S., Bartošková, Hana, Nauš, Jan, Ilík, Petr, Kouřil, Roman, Vidyasagar, P. B., Thomas, Sarah, Gaikwad, Jvoti U., Cseh, Z., Mustárdy, L., Garab, G., Simidjiev, I., Rajagopal, S., Várkonyi, Zs., Holzenburg, A., Stoylova, S., Papp, E., Millar, D. P., Bruder, R., Woo, T. T., Genick, U. K., Gerwert, K., Jávorfí, Tamás, Garab, Győző, Naqvi, Razi K., Gaikwad, Jyoti, Kalimullah, Md., Semwal, Manoj, Naus, Man, Ilik, Petr, Kouril, Roman, Horváth, Gábor, Bernard, Gary D., Pomozi, István, Wehner, Rüdiger, Damjanović, Ana, Schulten, Klaus, Ritz, Thorsten, Yandao, Gong, Jushuo, Wang, Nanming, Zhao, Jixiu, Shan, Freiberg, Arvi, Timpmann, Kõu, Woodbury, Neal W., Ruus, Rein, Nemtseva, E. V., Kudryasheva, N. S., Sizykh, A. G., Tikhomirov, A. A., Nesterenko, T. V., Shikhov, V. N., Forti, Giorgio, Furia, Alberto, Finazzi, Giovanni, Barbagallo, Romina Paola, Agalarov, R., Gasanov, R., Iskenderova, S., Nobuhiro, G. O., Osamu, Miyashita, Ramrakhiani, M., Soni, R. K., Yoshida, Masasuke, Akutsu, Hideo, Yagi, Hiromasa, Tozawa, Kacko, Sekino, Nobuaki, Iwabuchi, Tomoyuki, Kaulen, A. D., Avetisyan, A. V., Feniouk, B. A., Skulachev, V. P., Breyton, Cécile, Kühlbrandt, Werner, Gräslund, Astrid, Assarsson, Maria, Libisch, B., Horváth, G., Gombos, Z., Budagovskaya, N. V., Kudryasheva, N., Fukunishi, Arima, Harada, Erisa, Fukuoka, Yuki, Ohmura, Tomoaki, Kawai, Gota, Watanabe, Kimitsuna, Žekš, Boštjan, Božič, Bojan, Derganc, Jure, Svetina, Saša, Hoh, J. F. Y., Li, Z. B., Rossmanith, G. H., Frederix, P. L. T. M., de Beer, E. L., Treijtel, B. W., Blangè, T., Galtet, F., Hénon, S., Isabey, D., Planus, E., Laurent, V., Rath, L. S., Raval, M. K., Dash, P. K., Ramakrishnan, C., Balaram, R., Basak, Kanika, Balaban, Alexandra T., Nandy, Ashesh, Grunwald, Gregory D., Vracko, Marjan, Randic, Milan, Basak, Subhash C., Amic, Dragan, Beslo, Drago, Trinajstic, Nenad, Nikolic, Sonja, Walahaw, J., Lensink, Marc F. J., Reddy, Boojala V. B., Shindylov, Ilya N., Bourne, Philip E., Grigera, J. R., de Xammar Oro, J., Donnamaria, M. C., Neagu, Monica, Neagu, Adrian, Janežič, Dušanka, Praprotnik, Matej, Nilsson, Lennart, Mark, Pekka, Fata, La L., Dardenne, Laurent E., Werneck, Araken S., Neto, Marçal de O., Kannan, N., Vishveshwara, S., Veluraja, K., Opitz, David, Balasubramanian, Krishnan, Gute, Brian D., Mills, Denise, Lungeanu, Diana, Mihalas, G. I., Macovievici, G., Gruia, Raluca, Dalcin, B., Cortez-Maghelly, C., Passos, E. P., Ljubisavljevic, M., Blesic, S., Milosevic, S., Stratimirovic, D. J., Bachhawat, Nandita, Mande, Shekhar C., Nandy, A., Nishigaki, Koichi, Saito, Ayumu, Naimuddin, Mohammed, Takaesu, Hirotomo, Ono, Mitsuo, Hirokawa, Takatsugu, Eissa, A. M., Ahmed, Abdalla S., El Gohary, M. I., Nakashima, Hiroshi, Raghava, G. P. S., Kurgalvuk, N., Goryn, O., Gerstman, Bernard S., Kratasyuk, V. A., Esimbekova, E. N., Gritsenko, E. V., Remmel, N. N., Maznyak, O. M., German, A., Tikhonov, A., Tchitchkan, D., Koulchitsky, S., Pashkevich, S., Pletnev, S., Kulchitsky, V., Pesotskaya, Y., Shapiro, Erik M., Borthakur, Arijitt, Dimitrov, Ivan, Leigh, John S., Rizi, Rahim, Reddy, Ravinder, Charagundla, Sridhar, Duvvuri, Umamaheswar, Degaonkar, M., Khubchandani, M., Kumar, Mahesh, Jagannathan, N R., Raghunathan, P., Jayasundar, Rama, Coshic, O., Rath, O. K., Julka, P. K., Iliescu, Karina Roxana, Sajin, Maria, Petcu, Ileana, Moisoi, Nicolcta, Kuzmenko, A. I., Donchenko, G. V., Nikolenko, I. A., Morozova, R. P., Rahman, M. K., Ahmed, M. M., Watanabe, Takehiro, Uretzky, G., Ammar, R., Sharony, R., Rubin, Y., Gilboa, H., Mallick, H. N., Kumar, Mohan V., Begum, Gulnaz M., Degaonkar, Mahaveer N., Govindasamy, S., Kumosani, T. A., Lupusoru, C., Titescu, G., Haulica, I., Stefanescu, I., Iliescu, R., Nastasa, V., Bild, W., Khetawat, Gopal, Nealen, M., Faraday, N., Bray, P. F., Noga, S., Lycholat, E. A., Ananieva, T. V., Kosevich, M V., Stepanyan, S. G., Antonyuk, S. V., Khachatryan, A., Kumar, A., Arakelian, H., Khachatryan, R., Agadjanyan, S., Ayrapetyan, S., Mkheyan, V., Rajan, S. S., Kabaleeswaran, V., Gopalakrishnan, Geetha, Govindachari, T. R., Ramrakhiani, Meera, Cullen, David C., Lowe, Phillip, Badley, Andrew, Hermel, H., Möhwald, H., Schmahl, W., Singh, Anil K., Das, Joydip, Majumdar, Nirmalya, Dér, András, Oroszi, László, Kelemen, Loránd, Ormos, Pál, Hámori, András, Ramsden, Jeremy J., Mitra, Chanchal K., Savitri, D., Yanagida, Toshio, Esaki, Seiji, Sowa, Yosiyuki, Nishida, Tomoyuki, Kimura, Yuji, Radu, M., Laukhina, E. E., Kasumova, L. A., Koltover, V. K., Bubnov, V. P., Estrin, Ya. I., Dotta, Rajiv, Zahradník, Ivan, Marko, Milan, Novák, Pavel, Miyata, Hidetake, Hirata, Hiroaki, Sengupta, P., Maiti, S., Balaji, J., Banerjee, S., Barker, A. L., Winlove, C. P., OʼHare, D., Macpherson, J. V., Gonsalves, M., Unwin, P. R., Phillip, R., Kumar, Ravindra G., Murata, K., Nagayaka, K., Danev, R., Sugitani, S., Gősch, Michael, Thyberg, P., Földes-Papp, Z., Björk, G., Blom, H., Holm, J., Heino, T., Inagaki, Fuyuhiko, Yokochi, Masashi, Kusunoki, Masami, Matthews, E. K., Pines, J., Chukova, Yu. P., Koltover, Vitaly K., Kang, B. P. S., Bansal, Geetanjali, Bansal, M. P., Singh, U., Singh, Uma, Nakata, Kotoko, Nakano, Tastuya, Kaminuma, Tsuguchika, Kirn, Bonn, Potocnik, Neja, Stare, Vito, Shukla, Latal, Sastry, M. D., Natarajan, V., Devasagayam, T. P. A., Kesavan, P. C., Sayfutdinov, R., Degermendzhy, A. G., Adamovich, V. V., Rogozin, Yu. D., Khetrapal, C. L., Gowda, G. A. Nagana, Ghimire, Kedar Nath, Masaru, Ishida, Fujita, H., Ishiwata, S., Suzuki, M., Kawahara, S., Kirino, Y., Kishimoto, Y., Mori, H., Mishina, M., Ohshima, H., Dukhin, A. S., Goetz, P. J., Shilov, V. N., and Mishra, R. K.

Published
1999
Full Text
View/download PDF

26. Viral discovery and diversity in trypanosomatid protozoa with a focus on relatives of the human parasite Leishmania

Author

Grybchuk, Danyil, primary, Akopyants, Natalia S., additional, Kostygov, Alexei Y., additional, Konovalovas, Aleksandras, additional, Lye, Lon-Fye, additional, Dobson, Deborah E., additional, Zangger, Haroun, additional, Fasel, Nicolas, additional, Butenko, Anzhelika, additional, Frolov, Alexander O., additional, Votýpka, Jan, additional, d’Avila-Levy, Claudia M., additional, Kulich, Pavel, additional, Moravcová, Jana, additional, Plevka, Pavel, additional, Rogozin, Igor B., additional, Serva, Saulius, additional, Lukeš, Julius, additional, Beverley, Stephen M., additional, and Yurchenko, Vyacheslav, additional

Published
2017
Full Text
View/download PDF

35. Synthesis of novel derivatives of 5-carboxyuracil.

Author

Tauraitė, Daiva, Dabužinskaite, Julija, Ražanas, Rytis, Urbonavičius, Jaunius, Stankevičiūtė, Jonita, Serva, Saulius, and Meškys, Rolandas

Subjects
URACIL derivatives, CHEMICAL synthesis, ESTERS, DNA polymerases, REVERSE transcriptase inhibitors, THYMIDINE phosphorylase
Abstract

Copyright of Chemija is the property of Lithuanian Academy of Sciences Publishers and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Published
2015
Full Text
View/download PDF

40. Contributory presentations/posters

Author

Manoj, N., Srinivas, V., Surolia, A., Vijayan, M., Suguna, K., Ravishankar, R., Suguna, K., Surolia, A., Vijayan, M., Schwarzenbacher, R., Zeth, K., Diederichs, Kostner, G., Gries, A., Laggner, P., Prassl, R., Madhusudan, Akamine, Pearl, Xuong, Nguyen-huu, Taylor, Susan, Sagar, M., Ravishankar, R., Saikrishnan, K., Roy, S., Purnapatre, K., Handa, P., Varshney, U., Vijayan, M., Biswal, B., Sukumar, N., Vijayan, M., Rao, J., Johnson, A., Pattabhi, Vasantha, Krishna, S., Sastri, Mira, Savithri, H., Murthy, M., Pillai, Bindu, Kannan, Hosur, M., Kumar, Mukesh, Patwardhan, Swati, Kannan, K., Hosur, M., Padmanabhaa, B., Sasaki-Sugio, S., Nukaga, M., Matsuzaki, T., Karthikevan, S., Sharma, S., Sharma, A., Paramasivam, M., Kumar, P., Khan, J., Yadav, S., Srinivasan, A., Singh, T., Gourinath, S., Alam, Neelima, Srintvasan, A., Singh, T., Chandra, Vikas, Kaur, Punit, Betzel, Ch., Singh, T., Ghosh, S., Bera, A., Bhattacharya, S., Chakraborty, S., Pal, A., Mukhopadhyay, B., Dey, I., Haldar, U., Baneriee, Asok, Sevcik, Jozef, Solovicova, Adriana, Sekar, K., Sundaralingam, M., Betzel, Ch., Genov, N., Singh, T., Liang, Dong-cai, Jiang, Tao, Zhang, Ji-ping, Chang, Wen-rui, Jahnke, Wolfgang, Blommers, Marcel, Panchal, S., Hosur, R., Pillay, Bindu, Hosur, M., Mathur, Puniti, Srivatsun, S., Joshi, Ratan, Jaganathan, N., Chauhan, V., Atreya, H., Sahu, S., Chary, K., Govil, Girjesh, Adjadj, Elisabeth, Quinjou, Éric, Izadi-Pruneyre, Nadia, Blouquit, Yves, Mispelter, Joël, Heyd, Bernadette, Lerat, Guilhem, Milnard, Philippe, Desmadreil, Michel, Lin, Y., Rao, B., Raghunathan, Vidva, Chau, Mei, Rao, B., Pesais, Prashant, Srivastava, Sudha, Coutinho, Evans, Saran, Anil, Sapico, Leizl, Gesme, Jayson, Lijima, Herbert, Paxton, Raymond, Srikrishnan, Thamarapu, Grace, C., Nagenagowda, G., Lynn, A., Cowsik, Sudha, Sahu, Sarata, Chauhan, S., Bhattacharya, A., Chary, K., Govil, G., Kumar, Anil, Pellecchia, Maurizio, Zuiderweg, Erik, Kawano, Keiichi, Aizawa, Tomoyasu, Fujitani, Naoki, Hayakawa, Yoichi, Ohnishi, Atsushi, Ohkubo, Tadayasu, Kumaki, Yasuhiro, Hikichi, Kunio, Nitta, Katsutoshi, Rani Parvathy, V., Chary, K., Kini, R., Govil, G., Koshiba, Takumi, Kobashigawa, Yoshihiro, Yao, Min, Demura, Makoto, Nakagawa, Astushi, Tanaka, Isao, Kuwajima, Kunihiro, Nitta, Katsutoshi, Linge, Jens, Donoghue, Seán, Nilges, Michael, Chakshusmathi, G., Ratnaparkhi, Girish, Madhu, P., Varadarajan, R., Tetreau, C., Tourbez, M., Lavalette, D., Manno, M., Biagio, P., Martorana, V., Emanuele, A., Vaiana, S., Bulone, D., Palma-Vittorelli, M., Palma, M., Trivedi, V., Cheng, S., Chien, W., Yang, S., Francis, S., Chang, D., Batra, Renn, Geeves, Michael, Manstein, Dietmar, Trvlska, Joanna, Grochowski, Pawel, Geller, Maciej, Ginalski, K., Grochowski, P., Lesyng, B., Lavalette, P., Tetreau, C., Tourbez, M., Blouquit, Y., Roccatano, D., Amadei, A., Nola, A., Berendsen, H., Ho, Bosco, Curmi, P., Berry, H., Lairez, D., Pauthe, E., Pelta, J., Kothekar, V., Sahi, Shakti, Srinivasan, M., Singh, Anil, Madhusudnan, Kartha, Nandel, Fateh, Kaur, Harpreet, Nandel, Fateh, Singh, Balwinder, Jain, D., Feenstra, K., Berendsen, Herman, Tama, F., Sanejouand, Y., Go, N., Sharma, Deepak, Sharma, Sunita, Pasha, Santosh, Brahmachari, Samir, Viiavaraghavan, R., Makker, Jyoti, Dey, Sharmisllia, Kumar, S., Singh, T., Lakshmikanth, G., Krishnamoorthy, G., Mazhul, V., Zaitseva, E., Kierdaszuk, Borys, Widengren, J., Terry, B., Mets, Ü., Rigler, R., Swaminathan, R., Thamotharan, S., Yathindra, N., Shibata, Y., Chosrowjan, H., Mataga, N., Morisima, I., Chakraharty, Tania, Xiao, Ming, Cooke, Roger, Selvin, Paul, Branca, C., Faraone, A., Magazù, S., Maisano, G., Migliardo, P., Villari, V., Behere, Digambar, Deva, M., Brunori, M., Cutruzzolà, F., Gibson, Q., Savino, C., Travaglini-Allocatelli, C., Vallone, B., Prasad, Swati, Mazumdar, Shyamalava, Mitra, Samaresh, Soto, P., Fayad, R., Sukovataya, I., Tyulkova, N., Mamedov, Sh., Aktas, B., Canturk, M., Aksakal, B., Yilgin, R., Bogutska, K., Miroshnichenko, N., Chacko, S., DiSanto, M., Hypolite, J., Zheng, Y-M., Wein, A., Wojciechowski, M., Grycuk, T., Antosiewicz, J., Lesyng, B., Ceruso, Marc, Nola, Alfredo, Bandvopadhvay, Subhasis, Chatterjee, Bishnu, Choudhury, Devapriva, Thompson, Andrew, Stojanoff, Vivian, Pinkner, Jerome, Hultgren, Scott, Khight, Stefan, Flatters, Delphine, Goodfellow, Julia, Takazawatt, Fumi, Kanehisa, Minoru, Sasai, Masaki, Nakamura, Hironori, Sasai, Masaki, Han, Wang, Zheng, Yuan, Xin, Wang, Min, Pan, Bhakuni, Vlnod, Kulkarni, Sangeeta, Ahmad, Atta, Prakash, Koodathingal, Prajapati, Shashi, Surin, Alexey, Matsumoto, Tomoharu, Yang, Li, Nakagawa, Yuki, Kimura, Kazumoto, Amemiya, Yoshiyuki, Semisotnov, Gennady, Kihara, Hiroshi, Tayyab, Saad, Muzammil, Salman, Kumar, Yogesh, Kulkarni, Sangeeta, Prajapati, Shashi, Prakash, Koodathingal, Ahmad, Atta, Bhakuni, Vinod, Sundd, Monica, Kundu, Suman, Jagannadham, M., Kundu, Suman, Sundd, Monica, Jagannadham, Medicherla, Chandani, Bina, Dhar, Ruby, Sinha, Lalankumar, Warrier, Deepti, Mehrotra, Sonam, Khandelwal, Purnima, Seth, Subhendu, Hosur, R., Sasidhar, Y., Prabha, C., Gidwani, Arun, Ahmad, Atta, Kulkarni, Sangeeta, Madhusudan, K., Bhakuni, Vinod, Kinjo, Akira, Nishikawa, Ken, Chakravarty, Suvobrata, Varadarajan, Raghavan, Noyelle, K., Haezebrouck, P., Joniau, M., Dael, H., Dash, Sheffali, Jha, Indra, Bhat, Rajiv, Mohanty, Prasanna, Bandyopadhyay, A., Sonawat, H., Rao, Ch., Datta, Siddhartha, Rajaraman, K., Raman, B., Ramakrishna, T., Rao, Ch., Pande, A., Pande, J., Betts, S., Asherie, N., Ogun, O., King, J., Benedek, G., Sokolova, I., Tyulkova, N., Kalacheva, G., Sonoyama, Masashi, Yokoyama, Yasunori, Taira, Kunihiro, Mitaku, Shigeki, Nakazawal, Chicko, Sasakil, Takanori, Mukai, Yuri, Kamo, Naoki, Sonoyama, Masashi, Mitaku, Shigeki, Dalal, Seema, Regan, Lynne, Mukai, Yuri, Kamo, Naoki, Mituku, Shigeki, Roychoudhury, Mihir, Kumar, Devesh, Lőrinczv, Dénes, Könczöl, Franciska, Farkas, László, Belagyi, Joseph, Schick, Christoph, Thomson, Christy, Ananthanarayanan, Vettai, Alirzayeva, E., Baba-Zade, S., Gromiha, M., Oobatake, M., Kono, H., An, J., Uedaira, H., Sarai, A., Takano, Kazufumi, Yamagata, Yuriko, Yutani, Katsuhide, Jas, Gouri, Muñoz, Victor, Hofrichter, James, Eaton, William, Penoyar, Jonathan, Srikrishnan, Thamarapu, Lo Verde, Philip, Kardos, J., Bódi, Á., Venekei, I., Závodszky, P., Gráf, L., Szilágyi, András, Závodszky, Péter, Allan, R., Walshaw, J., Woolfson, D., Funahashi, Jun, Takano, Kazufumi, Yamagata, Yuriko, Yutani, Katsuhide, Gupta, Savan, Mazumdar, Shyamalava, Di Nola, A., Mangoni, M., Roccatano, P., Ramachandraiah, Gosu, Chandra, Nagasuma, Kothekar, V., Srinivasan, M., Sahi, Shakti, Chakraborty, S., Bhattacharya, S., Bera, A., Ghosh, S., Pal, A., Haldar, U., Mukhopadhyay, B., Baneriee, Asok, Ciani, Barbara, Woolfson, Derek, Nair, Usha, Kaur, Kanwal, Salunke, Dinakar, Swaminathan, Chittoor, Surolia, Avadhesha, Rigler, R., Pramanik, A., Jonasson, P., Kratz, G., Jansson, O., Nygren, P., Ståhl, S., Ekberg, K., Johansson, B., Uhlén, S., Uhlén, M., Jörnvall, H., Wahren, J., Welfle, Karin, Misselwitz, Rolf, Höhne, Wolfgang, Welfle, Heinz, Mazhul, V., Zaitseva, E., Mitskevich, L., Fedurkina, N., Kurganov, B., Jarori, Gotam, Maity, Haripada, Guharay, J., Sengupta, B., Sengupta, P., Sridevi, K., Kasturi, S., Gupta, S., Agarwal, Gunjan, Kwong, Suzanne, Briehl, Robin, Ismailova, O., N, Tyulkova, Hariharan, C., Pines, D., Pines, E., Zamai, M., Cohen-Luria, R., Yayon, A., Parola, A., Padya, M., Spooner, G., Woolfeon, D., Bakshi, Panchan, Sharma, Deepak, Sharma, Sunita, Bharadwaj, D., Pasha, Santosh, Sharma, U., Srivastava, N., Barthwal, R., Jagannathan, N., Matsuda, Keiko, Nishioka, Takaaki, Go, Nobuhiro, Aita, T., Urata, S., Husimi, Y., Majumder, Mainak, Chatterjee, Bishnu, Abrescia, Nicola, Malinina, Lucy, Subirana, Juan, Aymami, Juan, Eritxa, Ramón, Coll, Miquel, Premraj, B., Yathindra, N., Thenmalarchelvi, R., Yathindra, N., Kumar, P., Gautham, N., Kan, Lou, Ming-Hou, Lin, Shwu-Bin, Sana, Tapas, Roy, Kanal, Bruant, N., Flatters, D., Lavery, R., Genest, D., Rons, Remo, Sklenar, Heinz, Lavery, Richard, Kundu, Sudip, Bhattacharyya, Dhananjay, Bandyopadhyay, Debashree, Thakur, Ashoke, Majumdar, Rabi, Barceló, F., Portugal, J., Ramanathan, Sunita, Chary, K., Rao, B., Gliosli, Mahua, Kumar, N., Varshney, Umesh, Chary, K., Pataskar, Shashank, Brahmachari, Samir, Sarojini, R., Selvasekarapandian, S., Kolandaivel, P., Sukumar, S., Selvasekarapandian, S., Sarojini, R., Kolmdaivel, P., Sukumar, S., Sarojini, R., Selvasekarapandian, S., Kolandaivel, P., Sukumar, S., Selvasekarapandian, S., Sarojini, R., Kolandaivel, P., Sukumar, S., Maiti, Motilal, Sen, Anjana, Das, Suman, Terra, Elisa, Suraci, Chiara, Diviacco, Silvia, Quadrifoglio, Franco, Xodo, Luigi, Bandyopadhyay, Debashree, Bhattacharyya, Dhananjay, Kundu, Sudip, Thakur, Ashoke, Das, Suman, Ray, Arghya, Maiti, Motilal, Karthikeyan, G., Chary, Kandala, Rao, Basuthkar, Mujeeb, Anwer, James, Thomas, Kasyanenko, N., Haya, E., Bogdanov, A., Zanina, A., Bugs, M., Cornélio, M., Srikrishnan, Thamarapu, Tolstorukov, M., Sanval, Nitish, Tiwari, S., Tiwari, S., Sanyal, Nitish, Choudhury, Mihir, Kumar, Devesh, Sanyal, Nitish, Patel, P., Bhavesh, Neel, Hosur, R., Gabrielian, Anna, Wennmalm, Stefan, Edman, Lars, Rigler, Rudolf, Constantinescu, B., Radu, L., Radulcscu, I., Gazdaru, D., Wärmländer, Sebastian, Leijon, Mikael, Aoki, Setsuyuki, Kondo, Takao, Ishiura, Masahiro, Pashinskaya, V., Kosevich, M., Shelkovsky, V., Blagoy, Yu., Wang, Ji-hua, Malathi, R., Chandrasekhar, K., Premraj, B., Patel, P., Kandimalla, E., Agrawal, S., Hosur, R., Yathindra, N., Rastogi, V., Palafox, M., Singh, Chatar, Beniaminov, A., Bondarenko, S., Zdobnov, E., Minyat, E., Ulyanov, N., Ivanov, V., Singh, J., Sonawane, Kailas, Grosjean, Henri, Tewari, Ravindra, Sonavane, Uddhavesh, Morin, Annie, Grosjean, Henri, Tewari, Ravindra, Doherty, Elizabeth, Doudna, Jennifer, Tochio, H., Sato, S., Matsuo, H., Shirakawa, M., Kyogoku, Y., Javaram, B., Dixit, Surjit, Shukla, Piyush, Kalra, Parul, Das, Achintya, McConnell, Kevin, Beveridge, David, Sawyer, W., Chan, R., Eccelston, J., Yan, Yuling, Davidson, B., Ray, Arghya, Tuite, Eimer, Norden, Bengt, Nielsen, Peter, Takahashi, Masayuki, Ghosh, Anirban, Bansal, Manju, Christ, Frauke, Thole, Hubert, Wende, Wolfgang, Pingoud, Alfred, Pingoud, Vera, Luthra, Pratibha, Chandra, Ramesh, Sen, Ranjan, King, Rodney, Weisberg, Robert, Larsen, Olaf, Berends, Jos, Heus, Hans, Hilbers, Cornelis, Stokkum, Ivo, Gobets, Bas, Grondelle, Rienk, Amerongen, Herbert, Sngrvan, HE., Babayan, Yu., Khudaverdian, N., Kono, H., Gromiha, M., Pichierri, F., Aida, M., Prabakaran, P., Sayano, K., An, J., Uedaira, H., Sarai, A., Serva, Saulius, Merkienė, Eglė, Vilkaitis, Giedrius, Weinhold, Elmar, Klimašauskas, Saulius, Marsich, Eleonora, Bandiera, Antonella, Xodo, Luigi, Manzini, Giorgio, Potikyan, G., Arakelyan, V., Babayan, Yu., Ninaber, Alex, Goodfellow, Julia, Ito, Yoichiro, Ohta, Shigeru, Husimi, Yuzuru, Usukura, J., Tagami, H., Aiba, H., Suarez, Mougli, Nunes, Elia, Keszenman, Deborah, Candreva, E., Nunes, Elia, Thyberg, Per, Földes-Papp, Zeno, Rigler, Rudolf, Joshi, Amita, Rao, Basuthkar, Singh, Dinesh, Rajeswari, M., Ira, Krishnamoorthy, G., Pregetter, M., Prassl, R., Schwarzenbacher, R., Amenitsch, H., Chapman, J., Laggner, P., Pandev, B., Mishra, K., Pohl, E., Sun, J., Agapov, I., Tonevitsky, A., Pohl, P., Dennison, S., Guharay, J., Sengupta, P., Gorbeako, G., Dynbko, T., Pappavee, N., Mishra, A., Manuel, Prieto, Rodrigo, Almeida, Luis, Loura, Gendel, L., Przestalski, S., Kuczera, J., Kleszczyńska, H., Kral, T., Chernitsky, E., Senkovich, O., Rosin, V., Allakhverdieva, Y., Papageorgiou, G., Gasanov, R., Apetrei, Calin, Savopol, Tudor, Balea, Marius, Cucu, D., Mihailescu, D., Ramanathan, K., Bačić, Goran, Sajot, Nicolas, Garnier, Norbert, Crouzy, Serge, Genest, Monique, Várkonyi, Z., Zsiros, O., Farkas, T., Combos, Z., Cribier, Sophie, Fraceto, I., Schreier, S., Spisni, A., Paula, F., Sevšek, F., Gomišček, G., Arrigler, V., Svetina, S., Žekš, B., Nomura, Fumimasa, Nagata, Miki, Takiguchi, Kingo, Hotani, Hirokazu, Panicker, Lata, Parvathanathan, P., Ishino, A., Saitoh, A., Hotani, H., Takiguchi, K., Afonin, S., Takahashi, A., Nakato, Y., Takizawa, T., Marathe, Dipti, Mishra, K., Jørgensen, Kent, Rawat, Satinder, Nair, Usha, Rukmini, R., Chattopadhyay, Amitabha, Šentiurc, M., Štrancar, J., Stolič, Z., Filipin, K., Pečar, S., Chattopadhyay, Amitabha, Biswas, S., Rukmini, R., Sana, Satyen, Samanta, Anunay, Kinoshita, Koji, Yamazaki, Masahito, Ohba, Tetsuhiko, Kiuchi, Tai, Yoshitoshi, Kamakura, Goto, Akira, Kumeta, Takaaki, Ohki, Kazuo, Sugar, I., Thompson, T., Thompson, K., Biltonen, R., Suezaki, Y., Ichinose, H., Takiguchi, K., Hotani, H., Akivama, M., Matuoka, S., Tsuchihashi, K., Gasa, S., Mattjus, P., Molotkovsky, J., Pike, H., Brown, R., Arora, Ashish, Kleinschmidt, Jörg, Tamm, Lukas, Luneva, O., Gendel, L., Kruglyakova, K., Fedin, V., Kuptsoya, O., Borst, J., Visser, N., Visser, A., Dyubko, T., Ogihara, Toshihiko, Mishima, Kiyoshi, Shvaleva, A., Radenović, N., Minić, P., Jeremić, M., Radenović, Č., Aripov, T., Tadjibaeva, E., Vagina, O., Zamaraeva, M., Salakhutdinov, B., Cole, A., Poppofl, M., Naylor, C., Titball, R., Basak, A., Eaton, J., Naylor, C., Justin, N., Moss, D., Titball, R., Basak, A., Nomura, F., Nagata, M., Ishjkawa, S., Takiguchi, K., Takahashi, S., Hotani, H., Obuchi, Kaoru, Staudegger, Erich, Kriechbaum, Manfred, Lehrer, Robert, Waring, Alan, Lohner, Karl, Gangl, Susanne, Mayer, Bernd, Köhler, Gottfried, Shobini, J., Mishra, A., Guttenberg, Z., Lortz, B., Hu, B., Sackmann, E., Kozlova, N., Lukyanenko, L., Antonovich, A., Slobozhanina, E., Chernitsky, E., Krylov, Andrey, Antonenko, Yuri, Kotova, Elena, Yaroslavov, Alexander, Ghosh, Subhendu, Bera, Amal, Das, Sudipto, Urbánková, Eva, Jelokhani-Niaraki, Masood, Freeman, Karl, Jezek, Petr, Usmanov, P., Ongarbaev, A., Tonkikh, A., Pohl, Peter, Saparov, Sapar, Harikumar, P., Reeves, J., Rao, S., Sikdar, S., Ghatpande, A., Rao, S., Sikdar, S., Corsso, C., Campos de Carvalho, A., Varanda, W., ElHamel, C., Dé, E., Saint, N., Molle, G., Varshney, Anurae, Mathew, M., Loots, E., Isacoff, E., Kasai, Michiki, Yamaguchi, Naohiro, Ghosh, Paramita, Ghosh, Subhendu, Tigyi, Joseph, Tigyi, Gabor, Liliom, Karoly, Miledi, Ricardo, Djurisic, Maja, Andjus, Pavle, Shrivastava, Indira, Sansom, M., Barrias, C., Oliveira, P., Mauricio, A., Rebelo da Costa, A., Lopes, I., Barrias, C., Fedorovich, S., Chubanov, V., Sholukh, M., Konev, S., Fedirko, N., Manko, V., Klevets, M., Shvinka, N., Prabhananda, B., Kombrabail, Mamata, Aravamudhan, S., Venegas-Cotero, Berenice, Blake, Ivan, Zhang, Zhi-hong, Hu, Xiao-jian, Zhou, Han-qing, Cheng, Wei-ying, Feng, Hang-fang, Dubitsky, L., Vovkanvch, L., Zalyvsky, I., Savio-Galimberti, E., Bonazzola, P., Ponce-Homos, J., Parisi, Mario, Capurro, Claudia, Toriano, Roxana, Ready, Laxma, Jones, Larry, Thomas, David, Tashmukhamedov, B., Sagdullaev, B., Usmanov, P., Mauricio, A., Heitzmann, D., Warth, R., Bleich, M., Greger, R., Ferreira, K., Ferreira, H., Zagoory, Orna, Alfahel, Essa, Parola, Abraham, Priel, Zvi, Hama-Inaba, H., Wang, R., Choi, K., Nakajima, T., Haginoya, K., Mori, M., Ohyama, H., Yukawa, O., Hayata, I., Joshi, Nanda, Kannurpatti, Sridhar, Joshi, Preeti, Sinha, Mau, Shen, Xun, Hu, Tianhui, Bei, Ling, Knetsch, Menno, Schäfers, Nicole, Manstein, Dietmar, Sandblom, John, Galvanovskis, Juris, Pologea-Moraru, Roxana, Kovacs, Eugenia, Savopol, Tudor, Dinu, Alexandra, Sanghvi, S., Mishra, K., Jazbinšek, V., Thiel, G., Müller, W., Wübeller, G., Tronteli, Z., Fajmut, Leš, Marhl, Marko, Brumen, Milan, Volotovski, I., Sokolovski, S., Knight, M., Vasil’ev, Alexei, Chalyi, Alexander, Sharma, P., Steinbach, P., Sharma, M., Amin, N., Barchir, J., Albers, R., Pant, H., Balasubramanyam, M., Condrescu, M., Reeves, J., Gardner, J., Monajembashi, Shamci, Pilarczyk, Gotz, Greulich, K., Kovacs, Eugenia, El-Refaei, F., Talaat, M., El-Awadi, A., Ali, F., Tahradník, Ivan, Pavelková, Jana, Zahradniková, Alexandra, Zhorov, Boris, Ananthanaravanan, Vettai, Michailov, M., Neu, E., Seidenbusch, W., Gornik, E., Martin, D., Welscher, U., Weiss, D., Pattnaik, B., Jellali, A., Forster, V., Hicks, D., Sahel, J., Dreyfus, H., Picaud, S., Wang, Hong-Wei, Sui, Sen-fang, Luther, Pradeep, Barry, John, Morris, Ed, Squire, John, Sundari, C., Balasubramanian, D., Veluraia, K., Christlet, T., Suresh, M., Berry, H., Pelta, J., Lairez, D., Laretta-Garde, V., Krilov, Dubravka, Stojanović, Nataša, Herak, Janko, Jasuja, Ravi, Ivanova, Maria, Mirchev, Rossen, Ferrone, Frank, Stopar, David, Spruijt, Ruud, Wolfs, Cor, Hemminga, Marcus, Arcovito, G., Spirito, M., Sui, Sen-fang, Wang, Hong-Wei, Agrawal, Rajendra, Heagle, Amy, Penczek, Pawel, Grassucci, Robert, Frank, Joachim, Sharma, Manjuli, Jeyakumar, Loice, Fleischer, Sidney, Wagenknecht, Terence, Knupp, Carlo, Munro, Peter, Luther, Pradeep, Ezra, Eric, Squire, John, Ichihara, Koji, Kitazawa, Hidefumi, Iguchi, Yusuke, Hotani, Hirokazu, Itoh, Tomohiko, Pifat, Greta, Kveder, Marina, Pečar, Slavko, Schara, Milan, Nair, Deepak, Singh, Kavita, Rao, Kanury, Salunke, Dinakar, Kaur, Kanwaljeet, Jain, Deepti, Sundaravadivel, B., Goel, Manisha, Salunke, D., Kovalenko, E., Semenkova, G., Cherenkevich, S., Lakshmanan, T., Sriram, D., Srinivasan, S., Loganathan, D., Ramalingam, T., Lebrón, J., Bjorkman, P., Singh, A., Gayatri, T., Jain, Deepti, Kaur, Kanwaljeet, Sundaravadivel, B., Salunke, Dinakar, Caffarena, Ernesto, Grigera, J., Bisch, Paulo, Kiessling, V., Fromherz, P., Rao, K., Gaikwad, S., Khan, M., Suresh, C., Kaliannan, P., Gromiha, M., Elanthiraiyan, M., Chadha, K., Payne, J., Ambrus, J., Nair, M., Nair, Madhavan, Mahajan, S., Chadha, K., Hewitt, R., Schwartz, S., Bourguignon, J., Faure, M., Cohen-Addad, C., Neuburger, M., Ober, R., Sieker, L., Macherel, D., Douce, R., Gurumurthy, D., Velmurugan, S., Lobo, Z., Srivastava, Sudha, Phadke, Ratna, Govil, Girjesh, Desai, Prashant, Coutinho, Evans, Guseinova, I., Suleimanov, S., Zulfugarov, I., Novruzova, S., Aliev, J., Ismayilov, M., Savchenko, T., Alieva, D., Ilík, Petr, Kouřil, Roman, Bartošková, Hana, Nauš, Jan, Gaikwad, Jvoti, Thomas, Sarah, Vidyasagar, P., Garab, G., Simidjiev, I., Rajagopal, S., Várkonyi, Zs., Stoylova, S., Cseh, Z., Papp, E., Mustárdy, L., Holzenburg, A., Bruder, R., Genick, U., Woo, T., Millar, D., Gerwert, K., Getzoff, E., Jávorfí, Tamás, Garab, Győző, Naqvi, K., Kalimullah, Md., Gaikwad, Jyoti, Thomas, Sarah, Semwal, Manoj, Vidyasagar, P., Kouril, Roman, Ilik, Petr, Naus, Man, Pomozi, István, Horváth, Gábor, Wehner, Rüdiger, Bernard, Gary, Damjanović, Ana, Ritz, Thorsten, Schulten, Klaus, Jushuo, Wang, Jixiu, Shan, Yandao, Gong, Tingyun, Kuang, Nanming, Zhao, Freiberg, Arvi, Timpmann, Kõu, Ruus, Rein, Woodbury, Neal, Nemtseva, E., Kudryasheva, N., Sizykh, A., Shikhov, V., Nesterenko, T., Tikhomirov, A., Forti, Giorgio, Finazzi, Giovanni, Furia, Alberto, Barbagallo, Romina, Forti, Giorgio, Iskenderova, S., Agalarov, R., Gasanov, R., Osamu, Miyashita, Nobuhiro, G., Soni, R., Ramrakhiani, M., Yagi, Hiromasa, Tozawa, Kacko, Sekino, Nobuaki, Iwabuchi, Tomoyuki, Yoshida, Masasuke, Akutsu, Hideo, Avetisyan, A., Kaulen, A., Skulachev, V., Feniouk, B., Breyton, Cécile, Kühlbrandt, Werner, Assarsson, Maria, Gräslund, Astrid, Zsiros, O., Horváth, G., Mustárdy, L., Libisch, B., Gombos, Z., Budagovskaya, N., Kudryasheva, N., Harada, Erisa, Fukuoka, Yuki, Ohmura, Tomoaki, Fukunishi, Arima, Kawai, Gota, Watanabe, Kimitsuna, Akutsu, Hideo, Derganc, Jure, Božič, Bojan, Svetina, Saša, Žekš, Boštjan, Hoh, J., Li, Z., Rossmanith, G., Beer, E., Treijtel, B., Frederix, P., Blangè, T., Hénon, S., Galtet, F., Laurent, V., Planus, E., Isabey, D., Rath, L., Dash, P., Raval, M., Ramakrishnan, C., Balaram, R., Randic, Milan, Basak, Subhash, Vracko, Marjan, Nandy, Ashesh, Amic, Dragan, Beslo, Drago, Nikolic, Sonja, Trinajstic, Nenad, Walahaw, J., Woolfson, D., Lensink, Marc, Berendsen, Herman, Reddy, Boojala, Shindylov, Ilya, Bourne, Philip, Donnamaria, M., Xammar Oro, J., Grigera, J., Neagu, Monica, Neagu, Adrian, Praprotnik, Matej, Janežič, Dušanka, Mark, Pekka, Nilsson, Lennart, Martorana, V., Bulone, D., Fata, L., Manno, M., Biagio, P., Dardenne, Laurent, Werneck, Araken, Neto, Marçal, Bisch, Paulo, Kannan, N., Vishveshwara, S., Christlet, T., Veluraja, K., Grunwald, Gregory, Balaban, Alexandra, Basak, Kanika, Gute, Brian, Mills, Denise, Opitz, David, Balasubramanian, Krishnan, Mihalas, G., Lungeanu, Diana, Macovievici, G., Gruia, Raluca, Neagu, Monica, Cortez-Maghelly, C., Dalcin, B., Passos, E., Blesic, S., Ljubisavljevic, M., Milosevic, S., Stratimirovic, D., Bachhawat, Nandita, Mande, Shekhar, Ghosh, S., Nandy, A., Saito, Ayumu, Nishigaki, Koichi, Nishigaki, Koichi, Naimuddin, Mohammed, Mitaku, Shigeki, Hirokawa, Takatsugu, Ono, Mitsuo, Takaesu, Hirotomo, El Gohary, M., Ahmed, Abdalla, Eissa, A., Nakashima, Hiroshi, Nishikawa, Ken, Neagu, Monica, Neagu, Adrian, Raghava, G., Kurgalvuk, N., Goryn, O., Gerstman, Bernard, Gritsenko, E., Remmel, N., Maznyak, O., Kratasyuk, V., Esimbekova, E., Kratasyuk, V., Tchitchkan, D., Koulchitsky, S., Tikhonov, A., German, A., Pesotskaya, Y., Pashkevich, S., Pletnev, S., Kulchitsky, V., Duvvuri, Umamaheswar, Charagundla, Sridhar, Rizi, Rahim, Leigh, John, Reddy, Ravinder, Kumar, Mahesh, Coshic, O., Julka, P., Rath, O., Jagannathan, NR., Iliescu, Karina, Sajin, Maria, Moisoi, Nicolcta, Petcu, Ileana, Kuzmenko, A., Morozova, R., Nikolenko, I., Donchenko, G., Rahman, M., Ahmed, M., Naimuddin, Mohammed, Watanabe, Takehiro, Nishigaki, Koichi, Rubin, Y., Gilboa, H., Sharony, R., Ammar, R., Uretzky, G., Khubchandani, M., Mallick, H., Kumar, V., Jagannathan, N., Borthakur, Arijitt, Shapiro, Erik, Begum, M., Degaonkar, Mahaveer, Govindasamy, S., Dimitrov, Ivan, Kumosani, T., Bild, W., Stefanescu, I., Titescu, G., Iliescu, R., Lupusoru, C., Nastasa, V., Haulica, I., Khetawat, Gopal, Faraday, N., Nealen, M., Noga, S., Bray, P., Ananieva, T., Lycholat, E., Pashinskaya, V., Kosevich, MV., Stepanyan, S., Lycholat, E., Ananieva, T., Antonyuk, S., Khachatryan, R., Arakelian, H., Kumar, A., Ayrapetyan, S., Mkheyan, V., Agadjanyan, S., Khachatryan, A., Rajan, S., Kabaleeswaran, V., Malathi, R., Gopalakrishnan, Geetha, Govindachari, T., Ramrakhiani, Meera, Lowe, Phillip, Badley, Andrew, Cullen, David, Hermel, H., Schmahl, W., Möhwald, H., Singh, Anil, Majumdar, Nirmalya, Das, Joydip, Madhusudnan, Kartha, Dér, András, Kelemen, Loránd, Oroszi, László, Hámori, András, Ramsden, Jeremy, Ormos, Pál, Savitri, D., Mitra, Chanchal, Yanagida, Toshio, Esaki, Seiji, Kimura, Yuji, Nishida, Tomoyuki, Sowa, Yosiyuki, Radu, M., Koltover, V., Estrin, Ya., Kasumova, L., Bubnov, V., Laukhina, E., Dotta, Rajiv, Degaonkar, M., Raghunathan, P., Jayasundar, Rama, Jagannathan, N., Novák, Pavel, Marko, Milan, Zahradník, Ivan, Hirata, Hiroaki, Miyata, Hidetake, Ohki, Kazuo, Balaji, J., Sengupta, P., Maiti, S., Gonsalves, M., Barker, A., Macpherson, J., O’Hare, D., Winlove, C., Unwin, P., Sengupta, P., Phillip, R., Banerjee, S., Kumar, G., Maiti, S., Nagayaka, K., Danev, R., Sugitani, S., Murata, K., Gősch, Michael, Blom, H., Thyberg, P., Földes-Papp, Z., Björk, G., Holm, J., Heino, T., Rigler, Rudolf, Yokochi, Masashi, Inagaki, Fuyuhiko, Kusunoki, Masami, Matthews, E., Pines, J., Chukova, Yu., Koltover, Vitaly, Bansal, Geetanjali, Singh, Uma, Bansal, M., Nakata, Kotoko, Nakano, Tastuya, Kaminuma, Tsuguchika, Kang, B., Singh, U., Kirn, Bonn, Potocnik, Neja, Stare, Vito, Shukla, Latal, Natarajan, V., Devasagayam, T., Sastry, M., Kesavan, P., Sayfutdinov, R., Adamovich, V., Rogozin, D., Degermendzhy, A., Khetrapal, C., Ramanathan, K., Gowda, G., Ghimire, Kedar, Masaru, Ishida, Fujita, H., Ishiwata, S., Kishimoto, Y., Kawahara, S., Suzuki, M., Mori, H., Mishina, M., Kirino, Y., Ohshima, H., Dukhin, A., Shilov, V., Goetz, P., Sengupta, B., Guharay, J., Sengupta, P., and Mishra, R.

Published
1999
Full Text
View/download PDF

41. Synthesis of four colors fluorescently labelled 3'-O-blocked nucleotides with fluoride cleavable blocking group and linker for array based Sequencing-by-Synthesis applications

Author

Knapp, Diana C., Keller, Angelika, DOnofrio, Jennifer, Lubys, Arvydas, Serva, Saulius, Kurg, Ants, Remm, Maido, Kwiatkowski, Marek, and Engels, Joachim W.

Abstract

Reversible terminators having a fluoride cleavable 3′-O-blocking group are presented. Each nucleotide triphosphate is labelled by a fluorescent dye cleavable by the same reagent. We present here their synthesis, cleavage experiments and polymerase incorporation tests for a possible use in a process of Sequencing-by-Synthesis.

Published
2008
Full Text
View/download PDF

42. Sacharomyces cerevisiae L-A-1 viruso kapsidžių transfekcinio pajėgumo žinduolių ląstelėse HCT116 vertinimas

Author

Dulkytė, Rūta and Serva, Saulius

Subjects
viruses
Abstract

The yeasts Saccharomyces cerevisiae are safe organisms for humans. These yeasts have been used in the fermentation of various baked goods and beverages for thousands of years (Landry et al., 2006). A 2007 study of the human microbiome found that S. cerevisiae is also part of the natural microflora of healthy humans (Nash et al., 2017). This yeast is important and widely used in food (Legras et al., 2007), medicine, pharmaceutical industry and biofuel production (Türker, 2014). Saccharomyces cerevisiae is one of the model eukaryotic organisms that provides extensive knowledge of cellular processes (Landry et al., 2006). Most strains of Saccharomyces cerevisiae carry the L-A virus. These are 4.6 kb dgRNA viruses that are stably maintained in yeast cells and do not adversely affect their growth. L-A-1 capsid proteins have been found to form self-assembling VLPs (Fujimura et al., 1992). Such pseudovirion particles are applicable in the medical industry. One of the uses of VLPs is to adapt them to the development of various vaccines or delivery systems (Fuenmayor, Gòdia, & Cervera, 2017). The aim of this scientific work was to evaluate the possibility of applying L-A-1 virus VLPs as a nanocarriers. In particular, purification of VLP from the strains of S. cerevisiae BYΔLA, BYΔLA GagWT, BY4741 GagWT, and BY4741 GagΔ using the sucrose cushion method was used to confirm that L-A-1 virus components do not adversely affect the viability of the human cancer cell line HCT116. The highest levels of L-A-1 virus VLPs were found to be produced in the BY4741 ΔPOR1 GagWT strain, and the most efficient VLPs purification method was using PEG4000. Purification method using PEG4000 is 10 times more efficient than purification by sucrose gradient ultracentrifugation according to purified Gag protein concentration. Transfection capacity of purified viral particles showed that at low Dox concentrations (20 and 50 ng / ml), the cytotoxic effect of Dox on HCT116 cells is enhanced when the cells are co-cultured with L-A-1 virus VLP. Therefore, these particles may potentially improve the uptake of Dox into HCT116 cells at low concentrations (20 and 50 ng / ml), thus expanding the effective concentration spectrum of Dox. Monitoring of Dox fluorescence in exposed HCT116 cells also suggests that Dox enters cells efficiently when co-cultured with L-A-1 virus VLPs.

Published
2020

43. Application of modified nucleotides in biosynthesis of nucleic acids

Author

Mikalkėnas, Algirdas and Serva, Saulius

Subjects
modified nucleotides, modified nucleosides, reverse transcriptases, DNA polymerases
Abstract

Human immunodeficiency virus remains a serious threat for human health: over 36 million are infected with HIV, of which 1.8 million die each year. Retroviruses have a unique viral reproduction strategy employing reverse transcriptase for DNA biosynthesis from viral RNA. HIV 1 reverse transcriptase is among the most important targets for antiviral treatment. M. MuLV reverse transcriptase belongs to retroviral polymerases and, although structurally similar to HIV 1 reverse transcriptase, has distinct biochemical properties. These reverse transcriptases exhibit biochemical properties different from the host replication polymerase mode of action. The ability to utilize properties of polymerases would allow the development of inhibitors directed against viral polymerases without affecting of the host polymerases. In parallel, the ability of polymerases to use alternatively structured nucleotides would allow synthesizing DNA with additional functionalities. The use of modified nucleotides for DNA biosynthesis has become the subject of synthetic biology research in order to broaden the genetic code and create an artificial functional biological system with wider application possibilities. In this work, the use of modified pyridone nucleosides and nucleotides in DNA biosynthesis, as well as phosphonoacetic acid nucleotide conjugates designed to inhibit target DNA polymerases, are discussed.

Published
2019

44. Modifikuoti nukleotidai nukleorūgščių biosintezėje: taikomieji aspektai

Author

Mikalkėnas, Algirdas and Serva, Saulius

Subjects
modified nucleotides, modified nucleosides, reverse transcriptases, DNA polymerases
Abstract

Human immunodeficiency virus remains a serious threat for human health: over 36 million are infected with HIV, of which 1.8 million die each year. Retroviruses have a unique viral reproduction strategy employing reverse transcriptase for DNA biosynthesis from viral RNA. HIV 1 reverse transcriptase is among the most important targets for antiviral treatment. M. MuLV reverse transcriptase belongs to retroviral polymerases and, although structurally similar to HIV 1 reverse transcriptase, has distinct biochemical properties. These reverse transcriptases exhibit biochemical properties different from the host replication polymerase mode of action. The ability to utilize properties of polymerases would allow the development of inhibitors directed against viral polymerases without affecting of the host polymerases. In parallel, the ability of polymerases to use alternatively structured nucleotides would allow synthesizing DNA with additional functionalities. The use of modified nucleotides for DNA biosynthesis has become the subject of synthetic biology research in order to broaden the genetic code and create an artificial functional biological system with wider application possibilities. In this work, the use of modified pyridone nucleosides and nucleotides in DNA biosynthesis, as well as phosphonoacetic acid nucleotide conjugates designed to inhibit target DNA polymerases, are discussed.

Published
2019

45. Molecular determinants of Totiviridae family viruses of Saccharomyces sensu stricto clade

Author

Konovalovas, Aleksandras and Serva, Saulius

Subjects
viruses, Double-stranded RNA viruses, yeast viruses, host-factors, killer yeast
Abstract

In this research, we have discovered that L A virus is widespread among the most popular laboratory strains of S. cerevisiae yeast used in scientific research. The presence of L A virus is not associated with changes in cell viability or growth rate, however the data obtained in recent years show that the presence of the virus in the cell changes a large part of the host's gene expression. For this reason, transcriptomic or proteomic analysis data may be misinterpreted in the presence of a virus, because observed changes in gene expression may not necessarily be related to the studied process, but also may be related to the change in the amount and existence of the virus in the cell. In this work we have found new, unpublished host cell proteins interacting with proteins encoded by L-A virus. We have identified new L A virus and M satellite genomes from S. paradoxus and S. cerevisiae killer strains. We have found the direct relationship between the phylogenetic groups formed by different variants of the L A virus and the host species. Additionally, distribution in phylogenetic groups is based on strain origin and ability to provide a specific M satellite replication.

Published
2018

46. Mielių Saccharomyces sensu stricto Totiviridae šeimos virusų ciklo ląstelėje molekulinės determinantės

Author

Konovalovas, Aleksandras and Serva, Saulius

Subjects
viruses, Double-stranded RNA viruses, yeast viruses, host-factors, killer yeast
Abstract

In this research, we have discovered that L A virus is widespread among the most popular laboratory strains of S. cerevisiae yeast used in scientific research. The presence of L A virus is not associated with changes in cell viability or growth rate, however the data obtained in recent years show that the presence of the virus in the cell changes a large part of the host's gene expression. For this reason, transcriptomic or proteomic analysis data may be misinterpreted in the presence of a virus, because observed changes in gene expression may not necessarily be related to the studied process, but also may be related to the change in the amount and existence of the virus in the cell. In this work we have found new, unpublished host cell proteins interacting with proteins encoded by L-A virus. We have identified new L A virus and M satellite genomes from S. paradoxus and S. cerevisiae killer strains. We have found the direct relationship between the phylogenetic groups formed by different variants of the L A virus and the host species. Additionally, distribution in phylogenetic groups is based on strain origin and ability to provide a specific M satellite replication.

Published
2018

47. Mielių dvigrandinės RNR virusų klonavimas ir rekonstrukcija in vivo

Author

Survilaitė, Miglė and Serva, Saulius

Subjects
viruses
Abstract

Sacharomyces cerevisiae is one of the main model organisms for higher eukaryote research and investigation of different viruses. The dsRNA L-A virus of S. cerevisiae is widespread in nature. Four different types of L-A viruses (L-A, L-A-2, L-A-28, L-Alus) and one related L-BC virus are identified so far. Sometimes strains containing L-A virus also carry a satellite M dsRNA. These satellites encode a secreted protein toxin. There are several different types of M satellites (M-1, M-2, M-28, Mlus) encoding different killer toxins and immunity to that toxin. Yeast strains, which have L-A virus and M satellite, are able to kill other strains lacking these viruses. Up to now RNA virus research is complicated and expensive – there are no methods for direct RNA manipulations. Yeast virus L-A and its satellite M is a convenient model system for dsRNA reaserch. These viruses are not infectious, making them convenient to work with. Furthermore, the secreted killer toxin can be easily identified in S. cerevisiae cells. The aim of this research was to clone dsRNA from yeast viruses and sequence them. Using a method, which was developed in our laboratory, L-A viruses from strains used in biotechnology were cloned and identified. The second part of this research was to develop a trensfection method and create a killer phenotype in the yeast strains. Several different methods were used and evidence was obtained that some transfectants may be immune to the corresponding toxin, though further analysis is needed.

Published
2016

48. Lipase selection and application for fatty acid ester synthesis

Author

Šinkūnienė, Dovilė, SERVA, SAULIUS, BAGDONIENĖ, LIDA, ČITAVIČIUS, DONALDAS JONAS, JURKONIENĖ, SIGITA, SEREIKAITĖ, JOLANTA, MALINAUSKAS, ALBERTAS, BACHMATOVA, IRINA, and Vilnius University

Subjects
Fenetiloktanoatas, Response surface methodology, Atsako paviršiaus metodologija, Biodyzelinas, Acilgrupės migracija, Acyl migration, Lipase, Biodiesel, Phenethyloctanoate, Biochemistry, biodiesel, response surface methodology, acyl migration, phenethyloctanoate, Lipazės
Abstract

Lipazės yra intensyviai tiriama fermentų grupė dėl jų gebos katalizuoti platų spektrą sintezės reakcijų. Disertacinio darbo tikslas buvo ištirti ir optimizuoti riebalų rūgščių esterių sintezės, panaudojant lipazes, būdus. Tirta fermento preparato paruošimo (imobilizavimo), fermento bei substratų pasirinkimo bei reakcijos sąlygų ir priedų įtaka riebalų hidrolizės, biodyzelino, fenetiloktanoato (kvapiojo esterio) sintezės reakcijų eigai ir išeigai. Pirmą kartą ištirtos Lietuvoje paruoštos Enterobacter aerogenes lipazės savybės, ji imobilizuota ant gamtinės kilmės nešiklių. Komercinių lipazių katalizuojamų reakcijų sąlygų įtakai nustatyti ir reakcijos išeigai optimizuoti naudota atsako paviršiaus metodologija. Tiriant reakcijos priedų įtaką acilgrupės migracijai ir biodyzelino sintezės reakcijai, nustatyta, kad perspektyvus priedas yra silikagelis. Lipazių specifiškumas ženkliai skiriasi skirtingoms acilglicerolių klasėms ir regioizomerams, šių žinių pagrindu buvo pasiūlyta dviejų etapų biodyzelino sintezės katalizė. Lipases are widely researched enzyme group because of their ability to catalyze a wide range of synthesis reactions. The aim of this doctoral thesis was to study and optimize fatty acid ester synthesis using lipases. The influence of enzyme choice, its preparation (immobilization), choice of substrates and reaction conditions on fat hydrolysis, biodiesel and phenethyloctanoate (flavour ester) synthesis reaction course and yield was studied. Enterobacter aerogenes lipase, which was developed in Lithuania, was immobilized and its properties were determined for the first time. Commercial lipases were used for hydrolysis and synthesis reactions, the influence of reaction parameters was determined using response surface methodology, and so the yield was optimized. Silica gel was found to be a promising reaction additive for acyl group migration catalysis in biodiesel synthesis reaction. Different lipase specificities towards different acylglycerol classes and regioisomers varied greatly; on the basis of this knowledge two-step biodiesel synthesis catalysis was proposed.

Published
2014

49. Lipazių atranka ir taikymas riebalų rūgščių esterių sintezei

Author

Šinkūnienė, Dovilė, SERVA, SAULIUS, BAGDONIENĖ, LIDA, ČITAVIČIUS, DONALDAS JONAS, JURKONIENĖ, SIGITA, SEREIKAITĖ, JOLANTA, MALINAUSKAS, ALBERTAS, BACHMATOVA, IRINA, and Vilnius University

Subjects
Response surface methodology, Fenetiloktanoatas, Atsako paviršiaus metodologija, Biodyzelinas, Acilgrupės migracija, Acyl migration, Lipase, Biodiesel, Phenethyloctanoate, Biochemistry, biodiesel, response surface methodology, acyl migration, phenethyloctanoate, Lipazės
Abstract

Lipases are widely researched enzyme group because of their ability to catalyze a wide range of synthesis reactions. The aim of this doctoral thesis was to study and optimize fatty acid ester synthesis using lipases. The influence of enzyme choice, its preparation (immobilization), choice of substrates and reaction conditions on fat hydrolysis, biodiesel and phenethyloctanoate (flavour ester) synthesis reaction course and yield was studied. Enterobacter aerogenes lipase, which was developed in Lithuania, was immobilized and its properties were determined for the first time. Commercial lipases were used for hydrolysis and synthesis reactions, the influence of reaction parameters was determined using response surface methodology, and so the yield was optimized. Silica gel was found to be a promising reaction additive for acyl group migration catalysis in biodiesel synthesis reaction. Different lipase specificities towards different acylglycerol classes and regioisomers varied greatly; on the basis of this knowledge two-step biodiesel synthesis catalysis was proposed. Lipazių atranka ir taikymas riebalų rūgščių esterių sintezei Lipazės yra intensyviai tiriama fermentų grupė dėl jų gebos katalizuoti platų spektrą sintezės reakcijų. Disertacinio darbo tikslas buvo ištirti ir optimizuoti riebalų rūgščių esterių sintezės, panaudojant lipazes, būdus. Tirta fermento preparato paruošimo (imobilizavimo), fermento bei substratų pasirinkimo bei reakcijos sąlygų ir priedų įtaka riebalų hidrolizės, biodyzelino, fenetiloktanoato (kvapiojo esterio) sintezės reakcijų eigai ir išeigai. Pirmą kartą ištirtos Lietuvoje paruoštos Enterobacter aerogenes lipazės savybės, ji imobilizuota ant gamtinės kilmės nešiklių. Komercinių lipazių katalizuojamų reakcijų sąlygų įtakai nustatyti ir reakcijos išeigai optimizuoti naudota atsako paviršiaus metodologija. Tiriant reakcijos priedų įtaką acilgrupės migracijai ir biodyzelino sintezės reakcijai, nustatyta, kad perspektyvus priedas yra silikagelis. Lipazių specifiškumas ženkliai skiriasi skirtingoms acilglicerolių klasėms ir regioizomerams, šių žinių pagrindu buvo pasiūlyta dviejų etapų biodyzelino sintezės katalizė.

Published
2014

50. Kontroliuojamo aktyvumo restrikcijos endonukleazių-tripleksą formuojančių oligonukleotidų konjugatai

Author

Šilanskas, Arūnas, Sasnauskas, Kęstutis, Malinauskas, Albertas, Klimašauskas, Saulius, Lubys, Arvydas, Jakubauskas, Artūras, Daugelavičius, Rimantas, Serva, Saulius, and Vilnius University

Subjects
Gene therapy, gene therapy, restriction endonuclease, triplex forming oligonucleotide, Triplex forming oligonucleotide, Tripleksą formuojantis oligonukleotidas, Restriction endonuclease, Biochemistry, Genų taisymas, Restrikcijos endonukleazė
Abstract

Simple mutations within the coding region of critical human genes can lead to the formation of abnormal proteins, resulting in various diseases (e.g. cancer), in failure of an embryo to develop, or premature death. Genetic diseases can only be truly cured via restoration of defective gene function and one of the most promising strategies is based on homologous recombination. Naturally homologous recombination occurs with a low frequency (1 in 106 transfected cells), however it is known that DNA double-strand breaks enhance the efficiency of homologous recombination by several orders of magnitude (up to 10,000-fold). Therefore, gene therapy via homologous recombination requires new molecular tools that should be highly specific and rigorously controllable. In this work we have focused on the development of restriction enzyme-triple helix forming oligonucleotide (TFO) conjugates, where TFO provides specificity for the extended recognition site through the triple helix formation and addresses restriction enzyme to a particular target site where it introduces a double stranded break. We provide proof-of-concept demonstrations of two alternative strategies to control the DNA cleavage activity of restriction endonuclease-TFO conjugates, that allows adopt them in in vivo experiments. To this end we used restriction endonucleases MunI and Bse634I, which were structurally and biochemically characterized before in our laboratory. We successfully combined the restriction endonuclease... [to full text] Mutacijos, atsiradusios atitinkamuose žmogaus genuose, gali lemti pakitusių baltymų atsiradimą, kurie sukelia įvairias ligas (pvz.: vėžį), klaidingą embriono vystymąsi ar priešlaikinę mirtį. Tokios genetinės ligos gali būti gydomos genų terapijos būdu. Labiausiai vystoma genų terapijos strategija yra paremta homologine rekombinacija, kurios metu DNR seka, naudojama geno taisymui, yra patiekiama in trans. Natūraliai žinduolių ląstelėse homologinė rekombinacija (HR) vyksta žemu rekombinacijos dažniu (10-6). Tačiau yra žinoma, kad dvigrandininio trūkio įvedimas žymiai pagreitina HR (10-1). In vivo eksperimentų atveju dvigrandininio trūkio įvedimas turi būti ypač tikslus, todėl šis metodas reikalauja naujų molekulinių įrankių, kurie būtų itin specifiški ir griežtai kontroliuojami. Šiame darbe mes orientavomės į itin specifiškų ir griežtai kontroliuojamų meganukleazių kūrimą naudojant restrikcijos endonukleazių (REazių)-tripleksą formuojančių oligonukleotidų (TFO) konjugatus. REazių-TFO konjugatuose TFO suteikia specifiškumą prailgintam atpažinimo taikiniui per DNR triplekso susidarymą taip nukreipdamas restrikcijos fermentą prie konkretaus taikinio kur norima įvesti dvigrandininį trūkį. Šiuo tyrimu mes parodėme dvi alternatyvias restrikcijos endonukleazių-TFO konjugatų aktyvumo reguliavimo strategijas, kas leistų šias nukleazes panaudoti in vivo tyrimuose. Tuo tikslu buvo pasirinkti ortodoksiniai restrikcijos fermentai MunI ir Bse634I, kurie mūsų laboratorijoje yra gerai... [toliau žr. visą tekstą]

Published
2012

Catalog

Books, media, physical & digital resources
See catalog results