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Background: Autism spectrum disorder (ASD) is a neurodevelopmental disorder (NDD) characterized by social communication challenges and restricted, repetitive behaviors. While genetic and environmental factors are known to contribute to ASD, the role of the immune system remains unclear. This study investigated the separation patterns of serum and urine proteins in Nigerian children with ASD compared to typically developing children and children with other NDDs. Methods: Forty-seven participants aged 3–8 years were recruited, including 16 children diagnosed with ASD and 16 children with other NDDs, both according to DSM-5 criteria, along with 15 neurotypical children. Blood and urine samples were collected for protein analysis. Total protein and albumin levels were measured in both serum and urine using established methods. Protein separation in serum and urine was performed using cellulose acetate electrophoresis, followed by densitometry analysis of the electrophoretic patterns. Results: The results revealed no significant differences in total serum protein levels and most protein fractions between the groups. However, children with other NDDs exhibited significantly lower levels of alpha-2 globulin compared to neurotypical children. Conversely, both ASD and NDD groups showed significantly higher gamma globulin levels compared to the control group. Interestingly, spot urine protein levels were significantly higher in children with ASD compared to neurotypical children. Conclusion: The observed changes in alpha-2 and gamma globulin levels suggest potential immune system involvement in ASD and other NDDs. The higher urine protein excretion in the ASD group warrants further investigation to explore the potential of urinary protein biomarkers for ASD diagnosis. [ABSTRACT FROM AUTHOR]

Abstract Background Autism spectrum disorder (ASD) is a neurodevelopmental disorder (NDD) characterized by social communication challenges and restricted, repetitive behaviors. While genetic and environmental factors are known to contribute to ASD, the role of the immune system remains unclear. This study investigated the separation patterns of serum and urine proteins in Nigerian children with ASD compared to typically developing children and children with other NDDs. Methods Forty-seven participants aged 3–8 years were recruited, including 16 children diagnosed with ASD and 16 children with other NDDs, both according to DSM-5 criteria, along with 15 neurotypical children. Blood and urine samples were collected for protein analysis. Total protein and albumin levels were measured in both serum and urine using established methods. Protein separation in serum and urine was performed using cellulose acetate electrophoresis, followed by densitometry analysis of the electrophoretic patterns. Results The results revealed no significant differences in total serum protein levels and most protein fractions between the groups. However, children with other NDDs exhibited significantly lower levels of alpha-2 globulin compared to neurotypical children. Conversely, both ASD and NDD groups showed significantly higher gamma globulin levels compared to the control group. Interestingly, spot urine protein levels were significantly higher in children with ASD compared to neurotypical children. Conclusion The observed changes in alpha-2 and gamma globulin levels suggest potential immune system involvement in ASD and other NDDs. The higher urine protein excretion in the ASD group warrants further investigation to explore the potential of urinary protein biomarkers for ASD diagnosis.

This study determines the effects of probiotics - Saccharomyces cerevisiae on oocysts shedding, haematology and serum proteins of broilers infected with mixed Eimeria oocysts. 120-day-old broiler chicks were randomly assigned to four groups (Groups A - D) of 30 birds each. Group A - was fed a plain diet and not infected, Group B - was fed a supplemented diet and infected, Group C - was fed a plain diet and infected, and Group D - was fed a supplemented diet and not infected. S. cerevisiae was supplemented at 0.1 %/kg of feed. At week 3, Groups B and C were infected with sporulated mixed Eimeria oocysts orally, followed by a daily faecal examination of all groups. The rate of oocyst shedding, packed cell volume (PCV), haemoglobin concentration (Hb), red blood cell (RBC) count, total white blood cell (WBC) count, differential WBC count and serum levels of total protein, albumin and globulin were determined at five days' intervals. The data collected were analyzed using a one-way analysis of variance at a 95% confidence interval (p<0.05). Group C had the significantly higher (p<0.05) number of oocysts in faeces, total WBC count and globulin levels, with the lowest PCV, Hb, RBC count, total protein and albumin levels. Group D had significantly higher (p<0.05) heterophil, eosinophil and monocyte counts. In conclusion, dietary supplementation with S. cerevisiae (1 g/kg) ameliorated the effect of Eimeria infection in broilers, by reducing the rate of oocyst shedding, anaemia and hypoproteinaemia due to the activity of the parasite. [ABSTRACT FROM AUTHOR]

This document discusses the potential use of serum protein electrophoresis (SPE) as a screening tool for alpha-1 antitrypsin deficiency (AATD), a heritable condition that can lead to pulmonary and hepatic diseases. The study, conducted in four Italian hospitals, found that using the alpha1-globulin band in SPE can effectively detect AATD variants. The authors suggest that implementing SPE for population screening could lead to early intervention and improved outcomes for AATD patients. However, they note that SPE is not a diagnostic tool and cannot differentiate between different AATD variants or clinical phenotypes. Further research is needed to understand the clinical relevance of these findings. [Extracted from the article]

Pseudohyponatremia remains a problem for clinical laboratories. In this study, we analyzed the mechanisms, diagnosis, clinical consequences, and conditions associated with pseudohyponatremia, and future developments for its elimination. The two methods involved assess the serum sodium concentration ([Na]S) using sodium ion-specific electrodes: (a) a direct ion-specific electrode (ISE), and (b) an indirect ISE. A direct ISE does not require dilution of a sample prior to its measurement, whereas an indirect ISE needs pre-measurement sample dilution. [Na]S measurements using an indirect ISE are influenced by abnormal concentrations of serum proteins or lipids. Pseudohyponatremia occurs when the [Na]S is measured with an indirect ISE and the serum solid content concentrations are elevated, resulting in reciprocal depressions in serum water and [Na]S values. Pseudonormonatremia or pseudohypernatremia are encountered in hypoproteinemic patients who have a decreased plasma solids content. Three mechanisms are responsible for pseudohyponatremia: (a) a reduction in the [Na]S due to lower serum water and sodium concentrations, the electrolyte exclusion effect; (b) an increase in the measured sample's water concentration post-dilution to a greater extent when compared to normal serum, lowering the [Na] in this sample; (c) when serum hyperviscosity reduces serum delivery to the device that apportions serum and diluent. Patients with pseudohyponatremia and a normal [Na]S do not develop water movement across cell membranes and clinical manifestations of hypotonic hyponatremia. Pseudohyponatremia does not require treatment to address the [Na]S, making any inadvertent correction treatment potentially detrimental.

Alterations in the serum protein pattern may be associated with many diseases, including neoplastic processes. In veterinary medicine, these changes are poorly understood. Therefore, this study was aimed at the analysis of the distribution of blood serum protein fractions separated by agarose gel electrophoresis, and at the determination of the concentrations of main acute phase proteins in bitches with mammary gland neoplasia. The evaluation was conducted on twelve female dogs with palpable single or multiple nodules in the parenchyma of the mammary gland and on ten tumor-free clinically healthy bitches to compare the possible differences in the obtained results. Blood serum was used to perform agarose gel electrophoresis of the main blood serum protein fractions and to analyze the concentrations of total serum proteins and the following canine acute phase proteins: serum amyloid A, haptoglobin, C-reactive protein and α1-acid glycoprotein. The concentrations of total serum proteins were slightly higher in bitches with mammary gland tumors. Serum protein electrophoresis showed lower mean concentrations of albumin and α1-globulins in the affected dogs, while the concentrations of α2-and β1-globulins were significantly higher (P=0.0032 and P=0.0021, respectively) compared to dogs without mammary gland tumors. In the concentrations of acute phase proteins, significantly higher mean concentrations of C-reactive protein and haptoglobin were obtained in dogs with mammary tumors (P=0.0025 and P=0.0002, respectively). The values of α1-acid glycoprotein did not vary markedly between the bitches with and without mammary tumors. Presented data suggest that neoplastic processes in the mammary gland may also alter the electrophoretic pattern of blood serum proteins and induce changes in the production of some inflammatory proteins.

Organ functions are highly specialized and interdependent. Secreted factors regulate organ development and mediate homeostasis through serum trafficking and inter-organ communication. Enzyme-catalysed proximity labelling enables the identification of proteins within a specific cellular compartment. Here, we report a BirA*G3 mouse strain that enables CRE-dependent promiscuous biotinylation of proteins trafficking through the endoplasmic reticulum. When broadly activated throughout the mouse, widespread labelling of proteins was observed within the secretory pathway. Streptavidin affinity purification and peptide mapping by quantitative mass spectrometry (MS) proteomics revealed organ-specific secretory profiles and serum trafficking. As expected, secretory proteomes were highly enriched for signal peptide-containing proteins, highlighting both conventional and non-conventional secretory processes, and ectodomain shedding. Lower-abundance proteins with hormone-like properties were recovered and validated using orthogonal approaches. Hepatocyte-specific activation of BirA*G3 highlighted liver-specific biotinylated secretome profiles. The BirA*G3 mouse model demonstrates enhanced labelling efficiency and tissue specificity over viral transduction approaches and will facilitate a deeper understanding of secretory protein interplay in development, and in healthy and diseased adult states.

Rare-earth orthovanadate nanoparticles (NPs) demonstrate unique combination of physicochemical characteristics and biological activity. The redox properties and low toxicity of orthovanadates in nanoform allow considering them as perspective nanomedicine modalities. The local microenvironment in biological media and the way NPs enter the organism determine their biological efficiency. Numerous studies deal with interaction of vanadium species and bio-molecules in serum, however, the interplay between orthovanadate NPs and serum compounds is still under debates. Here we investigate the effect of serum proteins on aggregative stability of three types of negatively charged orthovanadate NPs (spherical, spindle-like, and rod-like). Size of NPs was estimated by light-scattering methods in solutions, biologically relevant buffers and media. Some geometry- and size-dependent peculiarities of NPs behavior in different solutions were found. Modeling the proteins–NPs interactions for main serum proteins at threshold Ca2+ and Mg2+ concentrations revealed nonlinear aggregative behavior depending on protein concentrations. In Krebs-Ringer buffer the behavior of the rod-like NPs differs strongly on the behavior of more isometric NPs (spherical and spindle-like). The hydrodynamic diameter changes are non-linear and rod-like NPs have specific behavior in serum-like protein mixtures. These effects are associated with shape-specific involvement of different forces (including electosteric, hydrophobic, osmotic and recoil ones) in stabilization process. [ABSTRACT FROM AUTHOR]

Spontaneous sorption of proteins on the nanoparticles' surface leads to the fact that nanoparticles in biological media are always enveloped by a layer of proteins—the protein corona. Corona proteins affect the properties of nanoparticles and their behavior in a biological environment. In this regard, knowledge about the composition of the corona is a necessary element for the development of nanomedicine. Because proteins have different sorption efficacy, isolating particles with a full corona and characterizing the full corona is challenging. In this study, we propose a photo-activated cross-linker for full protein corona fixation. We believe that the application of our proposed approach will make it possible to capture and visualize the full corona on nanoparticles coated with a lipid shell. [ABSTRACT FROM AUTHOR]

Simple Summary: Benign prostatic hyperplasia (BPH) is a common condition in older intact dogs, characterized by nonmalignant growth of the prostate gland. However, diagnosis and treatment strategies vary widely. Clinical proteomics is a rapidly growing field in both human and veterinary medicine. It has the potential to help us discover new therapeutic targets and biomarkers for diagnosis, prognosis, and treatment efficacy. However, there has been a notable gap in our understanding regarding changes in the serum proteome of dogs with BPH. This study aimed to compare serum proteome profiles of dogs with BPH to healthy dogs and evaluate the impact of castration. This research represents the first investigation into serum proteome profiling in dogs with BPH before and after castration. The findings suggest that specific serum proteins may be associated with the development and progression of canine BPH. These proteins hold the potential to serve as non-invasive biomarkers for diagnosing the disease. Further study is needed to identify and validate diagnostic biomarkers for BPH in dogs, including proteins involved in related pathways, as numerous alterations in serum proteins were observed post-castration. BPH is the most prevalent prostatic condition in aging dogs. Nevertheless, clinical diagnosis and management remain inconsistent. This study employed in-solution digestion coupled with nano-liquid chromatography tandem mass spectrometry to assess serum proteome profiling of dogs with BPH and those dogs after castration. Male dogs were divided into two groups; control and BPH groups. In the BPH group, each dog was evaluated at two time points: Day 0 (BF subgroup) and Day 30 after castration (AT subgroup). In the BF subgroup, three proteins were significantly upregulated and associated with dihydrotestosterone: solute carrier family 5 member 5, tyrosine-protein kinase, and FRAT regulator of WNT signaling pathway 1. Additionally, the overexpression of polymeric immunoglobulin receptors in the BF subgroup hints at its potential as a novel protein linked to the BPH development process. Conversely, alpha-1-B glycoprotein (A1BG) displayed significant downregulation in the BF subgroup, suggesting A1BG's potential as a predictive protein for canine BPH. Finasteride was associated with increased proteins in the AT subgroup, including apolipoprotein C-I, apolipoprotein E, apolipoprotein A-II, TAO kinase 1, DnaJ homolog subfamily C member 16, PH domain and leucine-rich repeat protein phosphatase 1, neuregulin 1, and pseudopodium enriched atypical kinase 1. In conclusion, this pilot study highlighted alterations in various serum proteins in canine BPH, reflecting different pathological changes occurring in this condition. These proteins could be a source of potential non-invasive biomarkers for diagnosing this disease. [ABSTRACT FROM AUTHOR]

Gastric cancer (GC) is a highly malignant disease affecting humans worldwide and has a poor prognosis. Most GC cases are detected at advanced stages due to the cancer lacking early detectable symptoms. Therefore, there is great interest in improving early diagnosis by implementing targeted prevention strategies. Markers are necessary for early detection and to guide clinicians to the best personalized treatment. The current semi-invasive endoscopic methods to detect GC are invasive, costly, and time-consuming. Recent advances in proteomics technologies have enabled the screening of many samples and the detection of novel biomarkers and disease-related signature signaling networks. These biomarkers include circulating proteins from different fluids (e.g., plasma, serum, urine, and saliva) and extracellular vesicles. We review relevant published studies on circulating protein biomarkers in GC and detail their application as potential biomarkers for GC diagnosis. Identifying highly sensitive and highly specific diagnostic markers for GC may improve patient survival rates and contribute to advancing precision/personalized medicine. [ABSTRACT FROM AUTHOR]

The measurement of serum IgG4 levels is mandatory for the diagnosis of IgG4-related disease, but no widely accepted reference material exists due to a lack of consensus on the standard assay. Therefore, we developed here an LC-MS/MS method for absolute quantification of IgG4 in a purified IgG sample, addressing a concern over the reliability depending on the proteolytic digestion efficiency. Our method uses internal calibrator sets containing unique amino acid sequences within IgG4, each of which comprises non-cleavable and dually-cleavable peptides labeled with different numbers of isotopes for mass separation, to determine digestion efficiency. Surrogate peptides generated by trypsin or lysyl endopeptidase digestion were selected based on selectivity, stability, and identifiability. IgG4 quantification using synthetic calibrator peptides showed high precision across the two conditions with different peptidases (relative differences ≤6.1%), even with low digestion efficiencies (<20%), which was within the interday precision under an established condition (% coefficient of variation ≤6.9%, digestion efficiencies >90%, n = 5). These results indicate that the LC-MS/MS method for quantifying IgG4 is robust against digestion efficiency variations and is applicable to validating an IgG4 reference material. • A reliable assay for IgG4 is needed for diagnosing IgG4-related disease (IgG4-RD). • Commercial IgG4 assays are calibrated to different reference materials and have generated inconsistent results. • MS quantification with a cleavable standard has proven robust against variations in the proteolytic digestion efficiency. • The MS-based method presented here is applicable to establishing a common calibrant for the standardization of IgG4 tests. [ABSTRACT FROM AUTHOR]

Bile acids regulate glucose homeostasis and lipid metabolism. Further, the levels of bile acids can be influenced by the intake of dairy products. Although the serum proteome can provide information on the biological pathways associated with different metabolites, it is unknown whether the intake of dairy modifies such associations between bile acids and the proteome. The objectives of this study were to examine plasma bile acid profiles, find the correlations between bile acids and lipid as well as glycemic markers, and to uncover the correlation between bile acids and proteins after high dairy (HD) and adequate dairy (AD) intake among 25 overweight individuals with hyperinsulinemia. In this randomized crossover-trial study, hyperinsulinemia adults were randomized to both HD (≥4 servings/day) and AD (≤2 servings/day) for 6 weeks. Measurements and analyses were performed on before- as well as after- AD and HD conditions. The results indicated that plasma 7α-hydroxy-4-cholesten-3-one (7AC4) increased after HD in comparison with before HD intake (p = 0.03). After adjusting for BMI, age, and sex, 7AC4 positively correlated with triglyceride levels in the pre-AD (r = 0.44; p = 0.03) and post-HD (r = 0.42; p = 0.04). Further, 7AC4 correlated positively with proteins associated with high-density lipoprotein particle remodeling pathway and reverse cholesterol transport only after HD consumption. Thus, the consumption of higher dairy intake modifies the association between 7AC4—a biomarker for bile acid synthesis—and serum proteins involved in cholesterol clearance. Overall, higher dairy consumption may have a positive effect on cholesterol metabolism in subjects at risk of type 2 diabetes. [ABSTRACT FROM AUTHOR]

Introduction: The binding of drugs to plasma proteins is an important consideration in drug development. We have reported that the dose of adenosine, lidocaine, and magnesium (ALM) fluid therapy for resuscitation from hemorrhagic shock is nearly 3‐times higher for pigs than rats. Since lidocaine strongly binds to serum alpha‐1‐acid glycoprotein (AGP), the aim of the study was to investigate the effect of hemorrhagic shock on levels of AGP in rats and pigs. Materials and Methods: Healthy adult male Sprague–Dawley rats and female crossbred pigs (n = 33 each) underwent tail vein and peripheral ear vein blood sampling, respectively, to collect plasma for AGP measurements. Rats (n = 17) and pigs (n = 16) underwent surgical instrumentation and uncontrolled hemorrhage via liver resection, and were treated with 3% NaCl ± ALM IV bolus followed 60 min later by 4 h 0.9% NaCl ± ALM IV drip. Rats were monitored for 72 h with blood samples taken post‐surgery, and at 5.25, 24, and 72 h. Pigs were monitored for 6 h with blood samples taken post‐surgery, and at 60 min and 6 h. Plasma AGP was measured with rat‐ and pig‐specific enzyme‐linked immunosorbent assay kits. Results: Baseline AGP levels in rats were 3.91 μg/mL and significantly 83‐fold lower than in pigs (325 μg/mL). Surgical instrumentation was associated with ~10‐fold increases in AGP in rats and a 21% fall in pigs. AGP levels remained elevated in rats after hemorrhage and resuscitation (28–29 μg/mL). In contrast, no significant differences in plasma AGP were found in ALM‐ or Saline‐treated pigs over the monitoring period. Conclusions: We conclude that the trauma of surgery alone was associated with significant increases in AGP in rats, compared to a contrasting decrease in pigs. Higher levels of plasma AGP in pigs prior to hemorrhagic shock is consistent with the higher ALM doses required to resuscitate pigs compared with rats. [ABSTRACT FROM AUTHOR]

Abstract Introduction The binding of drugs to plasma proteins is an important consideration in drug development. We have reported that the dose of adenosine, lidocaine, and magnesium (ALM) fluid therapy for resuscitation from hemorrhagic shock is nearly 3‐times higher for pigs than rats. Since lidocaine strongly binds to serum alpha‐1‐acid glycoprotein (AGP), the aim of the study was to investigate the effect of hemorrhagic shock on levels of AGP in rats and pigs. Materials and Methods Healthy adult male Sprague–Dawley rats and female crossbred pigs (n = 33 each) underwent tail vein and peripheral ear vein blood sampling, respectively, to collect plasma for AGP measurements. Rats (n = 17) and pigs (n = 16) underwent surgical instrumentation and uncontrolled hemorrhage via liver resection, and were treated with 3% NaCl ± ALM IV bolus followed 60 min later by 4 h 0.9% NaCl ± ALM IV drip. Rats were monitored for 72 h with blood samples taken post‐surgery, and at 5.25, 24, and 72 h. Pigs were monitored for 6 h with blood samples taken post‐surgery, and at 60 min and 6 h. Plasma AGP was measured with rat‐ and pig‐specific enzyme‐linked immunosorbent assay kits. Results Baseline AGP levels in rats were 3.91 μg/mL and significantly 83‐fold lower than in pigs (325 μg/mL). Surgical instrumentation was associated with ~10‐fold increases in AGP in rats and a 21% fall in pigs. AGP levels remained elevated in rats after hemorrhage and resuscitation (28–29 μg/mL). In contrast, no significant differences in plasma AGP were found in ALM‐ or Saline‐treated pigs over the monitoring period. Conclusions We conclude that the trauma of surgery alone was associated with significant increases in AGP in rats, compared to a contrasting decrease in pigs. Higher levels of plasma AGP in pigs prior to hemorrhagic shock is consistent with the higher ALM doses required to resuscitate pigs compared with rats.

Exosomes from peripheral serum of tuberculosis (TB) patients contain mycobacterial proteins, although the precise makeup of other exosomes is still unknown. In the current work, serum exosomes from individuals with active TB (ATB) underwent a thorough proteomics analysis. SDS PAGE was used to examine exosomes from ATB patients. This work identified several possible biomarkers linked to TB infection and gave a thorough description of the exosome proteome in the serum of ATB patients. [ABSTRACT FROM AUTHOR]

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The effect of replacing (5, 10, and 15%) wheat middlings in the feed of broiler chickens (EX5, EX10, EX15) with wheat germ expeller (WGE) on their parameters of carbohydrate, lipid, and protein metabolism was examined. Thirty-two randomly chosen broilers on day 43 were slaughtered, and their blood and liver were sampled. The concentration of glucose, triglycerides, total cholesterol, and protein and their fractions were tested in the blood serum. In livers, total protein and fat contents were analyzed. It has been found that replacing wheat middlings with 10% and 15% of expeller results in (p ≤ 0.05) a lower final body weight than CT. A higher feed, fat, protein, and methionine intake was noted in groups EX5-EX15 compared to CT. No alterations were found in the protein and fat content in the livers and the blood lipid profile of chickens. Changes in the protein metabolism of broilers indicate the need to research. WGE does not interfere with the metabolism of carbohydrates and lipids. However, WGE did not contribute to obtaining production benefits. [ABSTRACT FROM AUTHOR]

Ruthenium(III) complexes are very promising candidates as metal-based anticancer drugs, and several studies have supported the likely role of human serum proteins in the transport and selective delivery of Ru(III)-based compounds to tumor cells. Herein, the anticancer nanosystem composed of an amphiphilic nucleolipid incorporating a Ru(III) complex, which we named DoHuRu, embedded into the biocompatible cationic lipid DOTAP, was investigated as to its interaction with two human serum proteins thought to be involved in the mechanism of action of Ru(III)-based anticancer drugs, i.e., human serum albumin (HSA) and human transferrin (hTf). This nanosystem was studied in comparison with the simple Ru(III) complex named AziRu, a low molecular weight metal complex previously designed as an analogue of NAMI-A, decorated with the same ruthenium ligands as DoHuRu but devoid of the nucleolipid scaffold and not inserted in liposomal formulations. For this study, different spectroscopic techniques, i.e., Fluorescence Spectroscopy and Circular Dichroism (CD), were exploited, showing that DoHuRu/DOTAP liposomes can interact with both serum proteins without affecting their secondary structures. [ABSTRACT FROM AUTHOR]

Amyloid signature proteins such as serum amyloid P component, apolipoprotein E (ApoE), and ApoA-IV generally co-localise with amyloid, regardless of the types of amyloid precursor protein or the organs. Most of these proteins derive from serum and have reportedly been involved in amyloid fibril formation and stabilisation, as well as in excretion and degradation of amyloid precursor proteins. However, the processes and mechanisms by which these specific proteins deposit together with amyloid fibrils have not been clarified. We analysed the binding of serum proteins to amyloid fibrils derived from amyloid β and insulin in vitro by using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Specific serum proteins including ApoA-I, ApoE, ApoA-IV, ApoC-III and vitronectin adhered to amyloid fibrils at high concentrations in vitro. In addition, the profile of these proteins commonly occurred in both amyloid β and insulin amyloid fibrils and was mostly consistent with the composition of amyloid signature proteins. We also showed that high concentrations of serum proteins can adhere to amyloid fibrils in a short time. Our in vitro results suggest that amyloid signature proteins coexist with amyloid primarily dependent on the binding of each serum protein, in the extracellular fluid, to amyloid fibrils. [ABSTRACT FROM AUTHOR]

BPH is the most prevalent prostatic condition in aging dogs. Nevertheless, clinical diagnosis and management remain inconsistent. This study employed in-solution digestion coupled with nano-liquid chromatography tandem mass spectrometry to assess serum proteome profiling of dogs with BPH and those dogs after castration. Male dogs were divided into two groups; control and BPH groups. In the BPH group, each dog was evaluated at two time points: Day 0 (BF subgroup) and Day 30 after castration (AT subgroup). In the BF subgroup, three proteins were significantly upregulated and associated with dihydrotestosterone: solute carrier family 5 member 5, tyrosine-protein kinase, and FRAT regulator of WNT signaling pathway 1. Additionally, the overexpression of polymeric immunoglobulin receptors in the BF subgroup hints at its potential as a novel protein linked to the BPH development process. Conversely, alpha-1-B glycoprotein (A1BG) displayed significant downregulation in the BF subgroup, suggesting A1BG’s potential as a predictive protein for canine BPH. Finasteride was associated with increased proteins in the AT subgroup, including apolipoprotein C-I, apolipoprotein E, apolipoprotein A-II, TAO kinase 1, DnaJ homolog subfamily C member 16, PH domain and leucine-rich repeat protein phosphatase 1, neuregulin 1, and pseudopodium enriched atypical kinase 1. In conclusion, this pilot study highlighted alterations in various serum proteins in canine BPH, reflecting different pathological changes occurring in this condition. These proteins could be a source of potential non-invasive biomarkers for diagnosing this disease.

Spontaneous sorption of proteins on the nanoparticles’ surface leads to the fact that nanoparticles in biological media are always enveloped by a layer of proteins—the protein corona. Corona proteins affect the properties of nanoparticles and their behavior in a biological environment. In this regard, knowledge about the composition of the corona is a necessary element for the development of nanomedicine. Because proteins have different sorption efficacy, isolating particles with a full corona and characterizing the full corona is challenging. In this study, we propose a photo-activated cross-linker for full protein corona fixation. We believe that the application of our proposed approach will make it possible to capture and visualize the full corona on nanoparticles coated with a lipid shell.

It is well documented that choline is known as one of the essential ingredients of phospholipids. Choline acts as a determinative element for appropriate cell membrane functions. On the other hand α-tocopherol (Vit E) is a fat-soluble vitamin. This vitamin acts as a strong antioxidant in the living body's defense system against oxidative stress. Lipid peroxidation in peripartum and early lactating cows is significantly increased while the level of serum Vit E is decreases dramatically. These concomitant physiological changes demonstrate a higher level of oxidative stress subsequently leads to serious health issues in dairy cows. Therefore, the present research was designed to investigate the following items in dairy cattle: 1) evaluation of the possible changes in serum protein fractions, and 2) comparing the oxidative status of orally RPC and vitamin E supplementation in dairy cows in early lactation period. In the current study 30 early lactating primiparous and multiparous Holstein cows (body condition score (BCS)=2.51 ± 0.10) were used beginning five weeks postpartum. All the animals were randomly divided in to three groups (n=10) (number of lactation=2.61). The animals were randomly assigned to receive one of the following treatments. Group 1 served as control group were not received any supplement. The second group was supplemented with 90 g/d of RPC (Reashre Choline, Balchem, USA). The third group was administrated 4400 IU/d vitamin E (Roche, Vitamins Ltd; Switzerland). In the current study, serum protein electrophoresis showed four main fractions as follows: albumin, α-globulin, β-globulin, and γ-globulin. The recorded data showed that the percentages of albumin and γ-globulin fractions were higher in treated groups compared to the control group. In the animals supplementing with RPC and vitamin E the percentages of serum albumin increased to the value of 37. 70±1.63 and 38.21±1.28 respectively compare to the control group (34.69±1.21), which were significant (P

This trial was conducted to evaluate the effects of oregano essential oil supplementation at the concentration of 50 mL/1000L of drinking water on immune response and growth performance in broiler chickens. A total of 390 one-day-old Rose chickens were housed together in six experimental boxes (n=65 each), including non-vaccinated groups and non-vaccinated oregano treated groups. Group A: vaccinated with LaSota vaccine via eye drop at 5-, 15-, and 28-day-old adding oregano essential oil in drinking water 1 day post-vaccination. Group B: vaccinated with LaSota vaccine via eye drop at 5-, 15-, 28-day-old only without oregano essential oil. Group C: vaccinated with inactivated Newcastle disease (ND) vaccine at 5-day-old via subcutaneous (SC) injection one dose adding oregano essential oil in drinking water 1 day post-vaccination for 3 days. Group D: vaccinated with inactivated ND vaccine at 5-day-old via SC injection one dose only without oregano essential oil. Group E: non-vaccinated as the control group. Group F: non-vaccinated and adding oregano essential oil for 3 days in drinking water as the control group. The effect of oregano essential oil in drinking water combined with two forms of live and killed ND virus vaccines on the immune responses and serum proteins was investigated in broiler chickens. The results showed the significant effect of oregano on the immune response of chicks in groups D and C that were vaccinated with the killed vaccine at 23- and 33-day-old. Based on the findings, a peak was recorded at 38-day-old with a significant difference (P0.05) in the vaccinated broiler chicks, and serum globulin showed a quantitative increase in the vaccinated broiler chicks, compared with non-vaccinated groups. However, the results showed that the effect of oregano was non-significantly different (P>0.05) and it had no beneficial effect on growth performance. The results of this study showed the effects of commercial Oregano supplementation (50 mL/1000L drinking water), which increased serum total protein, including globulin, and provoked the best immunological response to ND vaccinations in broiler chickens. More research is needed to develop a state that may beneficially affect the health and uniformity of chickens.

Covid-19 is a global challenge that drives health care to its limits. Biological parameters associated with increased risk of severe or fatal disease courses were identified, including conditions connected with a poor state of health, organ damage, and coagulation dysfunction. In a recent study, risk factors like age, sex, blood group (ABO), smoking, and several biomarkers like white blood cells, lymphocytes, C-reactive protein (CRP), ferritin, D-dimer, and potassium level were considered. One hundred nasopharyngeal swabs and blood samples were collected from Iraqi patients and classified according to the severity of the disease into five groups: Asymptomatic, Mild, Moderate, Severe, and Deceased, according to the recommendations of the World Health Organization (WHO). The current study was based on the risk factors and their effect on disease severity (age, sex, blood type, and smoking). As a result, there was a substantial positive linear link (R2=0.91) between getting older and increasing disease severity. Study results were showed a little statistically significant difference in the number of males and females infected with SARS-CoV-2. Interestingly, males were shown to be more susceptible to infection than females. Also, because type (O) blood is more extensively distributed in the Middle East population than the other blood types, those with type (O) blood are more susceptible to infection. Because smokers and non-smokers were distributed at random across different disease severity levels, there was no statistically significant link between smoking status and COVID-19 severity. All parameters (plasma protein and liver enzymes) showed statistically significant differences, especially between the deceased groups except K level, which did not differ significantly in all groups. This study aimed to investigate some biological markers during infection to see if there was a link between these measurements and risk factors, as well as the severity and progression of the disease.

Summary: Background: Immune checkpoint inhibitors combined with chemotherapy as a neoadjuvant therapy have been applied to the treatment of esophageal squamous cell carcinoma (ESCC). However, the optimal regimen needs to be further explored, particularly for older patients, and the mechanisms by which the immune checkpoint inhibitor combined with chemotherapy modulates the evolution of ESCC are unknown. Methods: In this single-arm phase 2 trial, patients with resectable (stage II/III/IV without metastasis) ESCC were enrolled and received nanoparticle albumin-bound (nab) paclitaxel for two cycles and oral S-1 for 2 weeks, combined with intravenous toripalimab for two cycles before surgery. Combination postoperative adjuvant therapy was administered. The primary outcome was the major pathological response (MPR). Secondary outcomes included pathological complete response (pCR), overall response rate (ORR), disease control rate (DCR), disease-free survival (DFS), overall survival (OS), improvement in Stooler's dysphagia score and degree of daily living ability (dADL). Biopsies and plasma pre- and post-neoadjuvant therapy were performed using whole-exome sequencing, transcriptome sequencing, immunohistochemistry (IHC) for PD-L1, multiplex immunofluorescence (mIF) and proximity extension assay technology (PEA) for 92 proteins. Findings: From November 2019 to July 2021, 60 patients were enrolled. After neoadjuvant therapy, R0 resection was achieved in 55 (98.21%) patients. MPR was identified in 27 patients (49.09%), and 16 patients (29.09%) achieved pCR. Patients with PR, SD and PD were 37 (61.67%), 21 (35.00%) and 2 (3.33%), respectively. The overall staging, Stooler dysphagia scores and dADL were significantly decreased after treatment. 11 patients (18.3%) experienced grade ≥3 AEs. Compared to PD-L1-Low patients, PD-L1-High patients had a significantly higher ratio of PR. During therapy, the tumor mutation burden (TMB) and tumor neoantigen burden (TNB) were significantly decreased in patients with PR. Differential clonal evolution within tumors was demonstrated by analysis of intratumoral heterogeneity. Transcriptome analyses revealed that the infiltration of CD4+ T lymphocytes at baseline was associated with clinical outcome. During therapy, CD8+ T cells and CD4+ T cells were increased in all patients; however, exhausted cells, nTregs and iTregs were significantly increased in patients with non-MPR. Protein analyses revealed that the levels of IFN-γ, Gal.1 and LAMP3 can predict the clinical benefit. In addition, the expression of CD83, TNFRSF4, TNFSF14, VEGFR2, ADA, ARG1, and HO-1 was associated with serious AEs. More importantly, the integration of CD4+ T cells with plasma protein of IFN-γ, Gal.1 or LAMP3 could further distinguish responders from non-responders. Interpretation: In this study, neoadjuvant therapy with toripalimab, nab-paclitaxel and S-1 was less toxic and showed promising antitumor activity in patients with resectable ESCC. Changes in the genome, transcriptome, PD-L1 expression and serum proteins were comprehensively analyzed and correlated with clinical outcomes, which provides insight into the mechanism of action of toripalimab combined with nab-paclitaxel and S-1 in patients with ESCC. Funding: This study was funded by Major projects of the ministry of science and technology of the 13th five-year plan of China [grant number: 2018ZX09201013].

Aims: To assess the prognostic value of coronary computed tomography angiography (CTA) and serum biomarkers for the prediction of major adverse cardiac events (MACE) at three-month and one-year follow-ups.Methods and Results: A total of 720 patients with acute chest pain and normal electrocardiography (ECG) were included in the prospective cohort study. These patients received both coronary CTA screening and serum biomarkers testing, followed by three-month and one-year follow-ups for the occurrence of major adverse cardiac events (MACE). The primary outcome was the occurrence of MACE, which is defined as acute coronary syndrome (ACS), nonfatal MI, and all-cause mortality. The MACE rate was 17.8% (128 cases) and 25.2% (182 cases) at three-months and one-year follow-up. ApoB/apoA1(OR = 7.45, P < 0.001) and the number of atherosclerotic vessels (OR = 2.86, P < 0.001) were independent predictors for MACE at the three-month follow-up, so were apoB/apoA1 (OR = 5.23, P = 0.003), Serum amyloid protein A (SAA, OR = 1.04, P < 0.001) and the number of atherosclerotic vessels (OR = 2.54, P < 0.001) at the one-year follow-up. While apoB/apoA1 suggested its sensitivities of 84% for predicting MACE at three-month follow-ups, the number of atherosclerotic vessels had 81% specificity at one-year follow-up.Conclusions: Among patients with acute chest pain and normal ECG, apoB/apoA1, SAA and the number of atherosclerotic vessels are the most powerful predictors of MACE at three-month and one-year follow-ups. [ABSTRACT FROM AUTHOR]

Introduction. Gallic acid is a biologically active natural compound with strong antioxidant properties. Gallic acid is highly soluble and stable. It is known to increase the thermal stability of protein. However, its bioavailability is low, but interaction with proteins can solve this problem. Bovine serum albumin can bind various ligands, including polyphenols. The resulting complex of gallic acid and bovine serum albumin can become a promising functional food additive. Study objects and methods. This research featured in silico molecular modeling of gallic acid and bovine serum albumin using the HyperChem program. The methods of infrared spectrometry, potentiometry, and sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) made it possible to describe the physicochemical profile of the complex. Results and discussion. The molecular modeling confirmed that hydrophobic interactions were responsible for the chemical bond between gallic acid and bovine serum albumin. The SDS-PAGE test showed that the protein molecule remained intact. The reducing properties of the complex grew as the concentration of gallic acid increased. At 100 mg/L of gallic acid, the reducing properties were 7.8 ± 1.3 mg/L equivalent of gallic acid. At 200 and 300 mg/L, the values reached 15.90 ± 2.65 and 23.30 ± 5.05 mg/L, respectively. The IR spectrometry revealed a significant difference between the samples with different concentrations of gallic acid. Conclusion. The research managed to predict the properties of the complex of bovine serum albumin and gallic acid during its formation. The resulting complex had the highest reducing properties at 0.69 g of bovine serum albumin and 300 mg of gallic acid. The obtained parameters can be used in the food industry to develop new food additives.

Gastric cancer (GC) is a highly malignant disease affecting humans worldwide and has a poor prognosis. Most GC cases are detected at advanced stages due to the cancer lacking early detectable symptoms. Therefore, there is great interest in improving early diagnosis by implementing targeted prevention strategies. Markers are necessary for early detection and to guide clinicians to the best personalized treatment. The current semi-invasive endoscopic methods to detect GC are invasive, costly, and time-consuming. Recent advances in proteomics technologies have enabled the screening of many samples and the detection of novel biomarkers and disease-related signature signaling networks. These biomarkers include circulating proteins from different fluids (e.g., plasma, serum, urine, and saliva) and extracellular vesicles. We review relevant published studies on circulating protein biomarkers in GC and detail their application as potential biomarkers for GC diagnosis. Identifying highly sensitive and highly specific diagnostic markers for GC may improve patient survival rates and contribute to advancing precision/personalized medicine.

Bile acids regulate glucose homeostasis and lipid metabolism. Further, the levels of bile acids can be influenced by the intake of dairy products. Although the serum proteome can provide information on the biological pathways associated with different metabolites, it is unknown whether the intake of dairy modifies such associations between bile acids and the proteome. The objectives of this study were to examine plasma bile acid profiles, find the correlations between bile acids and lipid as well as glycemic markers, and to uncover the correlation between bile acids and proteins after high dairy (HD) and adequate dairy (AD) intake among 25 overweight individuals with hyperinsulinemia. In this randomized crossover-trial study, hyperinsulinemia adults were randomized to both HD (≥4 servings/day) and AD (≤2 servings/day) for 6 weeks. Measurements and analyses were performed on before- as well as after- AD and HD conditions. The results indicated that plasma 7α-hydroxy-4-cholesten-3-one (7AC4) increased after HD in comparison with before HD intake (p = 0.03). After adjusting for BMI, age, and sex, 7AC4 positively correlated with triglyceride levels in the pre-AD (r = 0.44; p = 0.03) and post-HD (r = 0.42; p = 0.04). Further, 7AC4 correlated positively with proteins associated with high-density lipoprotein particle remodeling pathway and reverse cholesterol transport only after HD consumption. Thus, the consumption of higher dairy intake modifies the association between 7AC4—a biomarker for bile acid synthesis—and serum proteins involved in cholesterol clearance. Overall, higher dairy consumption may have a positive effect on cholesterol metabolism in subjects at risk of type 2 diabetes.

Increasing dairy fat intake is expected to improve α-tocopherol bioavailability and could be beneficial for improving T2D risk factors. The aim of this study is to investigate the relationship between α-tocopherol levels and serum protein profiles before and after intake of adequate dairy (AD) or high dairy (HD) products in hyperinsulinemia patients. In this crossover study, dietary intake of 25 subjects with hyperinsulinemia (54 ± 14 yrs and BMI 31 ± 3) included: 1- pre-AD intake; 2- pre-HD intake; 3- post- AD intake (≤2 servings/day); and 4- post-HD intake (≥4 servings/day) assessed by food frequency questionnaire. In pre-AD, post-AD and pre-HD, α-tocopherol positively correlated with proteins mainly involved in lipid metabolism (rho > 0.40; P

Flonicamid is an insecticide which is frequently used in agriculture practices these days. However the excessive use of this chemical insecticide exhibit toxic effects on ecosystem as well as on mammals. Therefore, the present study was conducted to evaluate flonicamid median lethal dose which is 180 mg/kg body weight. Further, investigations involved the repeated oral administration of safe dose of flonicamid i.e 18 mg/kg body weight/day to various groups of rabbits alone, in combination with vitamin C and E for partial sub chronic study of 30 days. Significant increase (P<0.05) in hepatic enzymes such as alanine aminotransferase, aspartate aminotransferase and alkaline phosphatase were observed whereas animal body weight, serum protein and serum albumin exhibited significant decrease (P<0.05) in rabbits given only flonicamid. In addition, histological examination of liver tissues of treated rabbits showed alterations of hepatocytes morphology from severe level to mild depending upon type of treatment either given flonicamid only or co-administered with vitamin C/vitamin E. We conclude that supplementation of vitamin C alone and in combination with vitamin E each at 100 mg/kg body weight/day dose significantly reduces the toxicopathetic effects of flonicamid in exposed rabbits and ameliorates the hepatic marker enzymes as well as histological aspects of liver tissues near the control group. [ABSTRACT FROM AUTHOR]

Background: Recent genetic and transcriptomic data highlight the need for improved molecular characterisation of inflammatory bowel disease (IBD). Proteomics may advance the delineation of IBD phenotypes since it accounts for post-transcriptional modifications., Aim: We aimed to assess the IBD spectrum based on inflammatory serum proteins and identify discriminative patterns of underlying biological subtypes across multiple European cohorts., Methods: Using proximity extension methodology, we measured 86 inflammation-related serum proteins in 1551 IBD patients and 312 healthy controls (HC). We screened for proteins exhibiting significantly different levels among IBD subtypes and between IBD and HC. Classification models for differentiating between Crohn's disease (CD) and ulcerative colitis (UC) were employed to explore the IBD spectrum based on estimated probability scores., Results: Levels of multiple proteins, such as IL-17A, MMP-10, and FGF-19, differed (fold-change>1.2; FDR<0.05) between ileal vs colonic IBD. Using multivariable models, a protein signature reflecting the IBD spectrum was identified, positioning colonic CD between UC and ileal CD, which were at opposite ends of the spectrum. Based on area under the curve (AUC) estimates, classification models more accurately differentiated UC from ileal CD (median AUCs>0.73) than colonic CD (median AUCs<0.62). Models differentiating colonic CD from ileal CD demonstrated intermediate performance (median AUCs 0.67-0.69)., Conclusion: Our findings in serum proteins support the presence of a continuous IBD spectrum rather than a clear separation of CD and UC. Within the spectrum, disease location may reflect a more similar disease than CD vs UC, as colonic CD resembled UC more closely than ileal CD., (© The Author(s) 2024. Published by Oxford University Press on behalf of European Crohn’s and Colitis Organisation.)

Anesthetics penetrate the blood-brain-barrier (BBB) and - as confirmed preclinically – transiently disrupt it. An analogous consequence in humans has remained unproven. In mice, we previously reported that upon BBB dysfunction, the brain acts as ‘immunoprecipitator’ of autoantibodies against N-methyl-D-aspartate-receptor subunit-NR1 (NMDAR1-AB). We thus hypothesized that during human anesthesia, pre-existing NMDAR1-AB will specifically bind to brain. Screening of N = 270 subjects undergoing general anesthesia during cardiac surgery for serum NMDAR1-AB revealed N = 25 NMDAR1-AB seropositives. Only N = 14 remained positive post-surgery. No changes in albumin, thyroglobulin or CRP were associated with reduction of serum NMDAR1-AB. Thus, upon anesthesia, BBB opening likely occurs also in humans.

Breast cancer (BC) is the most common cancer and among the leading causes of cancer death in women. It is a heterogeneous group of tumours with numerous morphological and molecular subtypes, making predictions of disease evolution and patient outcomes difficult. Therefore, biomarkers are needed to help clinicians choose the best treatment for each patient. For the last years, studies have increasingly focused on biomarkers obtainable by liquid biopsy. Circulating proteins (from serum or plasma) can be used for inexpensive and minimally invasive determination of disease risk, early diagnosis, treatment adjusting, prognostication and disease progression monitoring. We provide here a review of the main published studies on serum proteins in breast cancer and elaborate on the potential of circulating proteins to be predictive and/or prognostic biomarkers in breast cancer. [ABSTRACT FROM AUTHOR]

Background: Dexamethasone is not only a potent glucocorticoid with several health benefits but is also associated with severe side effects, one of which is hepatotoxicity. L-Citrulline is known to possess antioxidant, antidiabetic, and antidyslipidemic effects, among others, of which hepatoprotection has not been extensively explored. We aimed to assess the effect of L-Citrulline on dexamethasone-induced derangement in liver enzymes and serum proteins in Wistar rats. Materials and Methods: Twenty-five male Wistar rats, weighing between 200 and 250 g, were randomly assigned into five groups of five rats each. While Group I received no intervention, dexamethasone intraperitoneally (1 mg/kg) was administered to the other groups for 7 days. Groups III, IV, and V were pretreated with 200, 400, and 800 mg/kg L-Citrulline daily for 21 days, respectively. Biochemical assessment was made after humanely sacrificing the animals. Values at P < 0.05 were considered statistically significant compared to the dexamethasone group. Results: L-Citrulline significantly lowered the levels of aspartate transferase (AST), alanine transferase (ALT), gamma–glutamyltransferase, and serum total and conjugated bilirubin in a dose-dependent manner. The greatest reduction in alkaline phosphatase level by L-Citrulline was recorded at 200 mg/kg (13.96 ± 0.73 IU/L). Similarly, the total protein level was significantly increased by L-Citrulline 800 mg/kg (9.38 ± 0.39 g/dL), but the greatest increase in albumin level was at 400 mg/kg (4.20 ± 0.21 g/dL). In a dose-dependent manner, the AST: ALT ratios were markedly reduced while the albumin: globulin ratios were greatly increased following L-Citrulline supplementation. Conclusion: L-Citrulline supplementation confers hepatoprotective effect against dexamethasone-induced derangements in liver enzymes and serum proteins in Wistar rats. [ABSTRACT FROM AUTHOR]

Identification and use of non-invasive biomarkers for kidney transplantation monitoring is an unmet need. A total of 121 biobanked sera collected from 111 unique kidney transplant (KT) patients (children and adolescent) and 10 age-matched healthy normal controls were used to profile serum proteins using semi-quantitative proteomics. The proteomics data were analyzed to identify panels of serum proteins that were specific to various transplant injuries, which included acute rejection (AR), BK virus nephropathy (BKVN), and chronic allograft nephropathy (CAN). Gene expression data from matching peripheral blood mononuclear cells were interrogated to investigate the association between soluble serum proteins and altered gene expression of corresponding genes in different injury phenotypes. Analysis of the proteomics data identified from different patient phenotypes, with criteria of false discovery rate

Organ functions are highly specialized and interdependent. Secreted factors regulate organ development and mediate homeostasis through serum trafficking and inter-organ communication. Enzyme-catalysed proximity labelling enables the identification of proteins within a specific cellular compartment. Here, we report a BirA*G3 mouse strain that enables CRE-dependent promiscuous biotinylation of proteins trafficking through the endoplasmic reticulum. When broadly activated throughout the mouse, widespread labelling of proteins was observed within the secretory pathway. Streptavidin affinity purification and peptide mapping by quantitative mass spectrometry (MS) proteomics revealed organ-specific secretory profiles and serum trafficking. As expected, secretory proteomes were highly enriched for signal peptide-containing proteins, highlighting both conventional and non-conventional secretory processes, and ectodomain shedding. Lower-abundance proteins with hormone-like properties were recovered and validated using orthogonal approaches. Hepatocyte-specific activation of BirA*G3 highlighted liver-specific biotinylated secretome profiles. The BirA*G3 mouse model demonstrates enhanced labelling efficiency and tissue specificity over viral transduction approaches and will facilitate a deeper understanding of secretory protein interplay in development, and in healthy and diseased adult states.

Abstract Background The possibility to monitor patient's serum proteome during treatment can provide deepened understanding of the biology associated with response to specific drugs. Non‐invasive serum sampling provides an opportunity for sustainable repetitive sampling of patients, which allows for more frequent evaluation of the biological response and enhanced flexibility in treatment selection in contrast to tissue biopsies. Aim To pin‐point biologically relevant changes in pre‐ and on‐treatment serum proteome samples in relapsed mantle cell lymphoma (MCL) patients, leading to insight into mechanisms behind response to treatment in sub‐groups of patients. Methods Pre‐ and on‐treatment serum samples from relapsed MCL patients treated with a triple combination therapy of rituximab, ibrutinib and lenalidomide were available for the study, together with detailed clinicopathological information. A microarray technology targeting 158 serum proteins using 371 antibody‐fragments was used to compare the serum proteome at the two time‐points. Results Proteins modulated by the treatment were shown to be associated to a MCL sub‐group with ATM/TP53 alterations, which emphasizes the importance of treatment stratification. Absolute values of serum protein levels in on‐treatment samples were highly variable and showed no correlation to outcome. To circumvent the challenge of variability in absolute serum protein levels, the velocity of change of individual serum proteins was used to identify proteins associated with clinical response. Increased values of TGF‐β1, CD40 and complement component 4 comparing pre‐ and on‐treatment samples were associated with remaining minimal residual disease (MRD) and increased BTK was associated with short progression‐free survival (PFS). Conclusion We show that the genetic sub‐type of MCL affects the biological response to treatment in serum and that the change in defined serum proteins reveals the biology associated with clinical response.

INTRODUCTION: In DM, changes in blood serum parameters can be observed in addition to blood glucose level. In the treatment of this disease, it is used in traditional herbal treatments besides the drugs known. This study was carried out to investigate the effects of two different thyme (TVL: Thymus vulgaris L., TSL: Thymbra spicata L.) species on serum proteins of diabetic rats. METHODS: Groups; control, diabetes, diabetes and glibenclamide given, diabetes+TVL 100 mg/kg, diabetes+TVL 200 mg/kg, diabetes+TSL 100 mg/kg, diabetes+TSL 200 mg/kg. The serum proteins were fractionated by cellulose acetate electrophoresis method. The acetate electrophoresis cards were scanned software and the concentrations (g/L) and ratios (%) of the study groups were determined. RESULTS: There was no difference between control and experimental diabetes in all parameters. it was determineted that: total protein and α1-globulin; decreased significantly in TVL2 and TSL2 groups (p

The effect of replacing (5, 10, and 15%) wheat middlings in the feed of broiler chickens (EX5, EX10, EX15) with wheat germ expeller (WGE) on their parameters of carbohydrate, lipid, and protein metabolism was examined. Thirty-two randomly chosen broilers on day 43 were slaughtered, and their blood and liver were sampled. The concentration of glucose, triglycerides, total cholesterol, and protein and their fractions were tested in the blood serum. In livers, total protein and fat contents were analyzed. It has been found that replacing wheat middlings with 10% and 15% of expeller results in (p ≤ 0.05) a lower final body weight than CT. A higher feed, fat, protein, and methionine intake was noted in groups EX5-EX15 compared to CT. No alterations were found in the protein and fat content in the livers and the blood lipid profile of chickens. Changes in the protein metabolism of broilers indicate the need to research. WGE does not interfere with the metabolism of carbohydrates and lipids. However, WGE did not contribute to obtaining production benefits.

Ruthenium(III) complexes are very promising candidates as metal-based anticancer drugs, and several studies have supported the likely role of human serum proteins in the transport and selective delivery of Ru(III)-based compounds to tumor cells. Herein, the anticancer nanosystem composed of an amphiphilic nucleolipid incorporating a Ru(III) complex, which we named DoHuRu, embedded into the biocompatible cationic lipid DOTAP, was investigated as to its interaction with two human serum proteins thought to be involved in the mechanism of action of Ru(III)-based anticancer drugs, i.e., human serum albumin (HSA) and human transferrin (hTf). This nanosystem was studied in comparison with the simple Ru(III) complex named AziRu, a low molecular weight metal complex previously designed as an analogue of NAMI-A, decorated with the same ruthenium ligands as DoHuRu but devoid of the nucleolipid scaffold and not inserted in liposomal formulations. For this study, different spectroscopic techniques, i.e., Fluorescence Spectroscopy and Circular Dichroism (CD), were exploited, showing that DoHuRu/DOTAP liposomes can interact with both serum proteins without affecting their secondary structures.

This trial was conducted to evaluate the effects of oregano essential oil supplementation at the concentration of 50 mL/1000L of drinking water on immune response and growth performance in broiler chickens. A total of 390 one-day-old Rose chickens were housed together in six experimental boxes (n=65 each), including nonvaccinated groups and non-vaccinated oregano treated groups. Group A: vaccinated with LaSota vaccine via eye drop at 5-, 15-, and 28-day-old adding oregano essential oil in drinking water 1 day post-vaccination. Group B: vaccinated with LaSota vaccine via eye drop at 5-, 15-, 28-day-old only without oregano essential oil. Group C: vaccinated with inactivated Newcastle disease (ND) vaccine at 5-day-old via subcutaneous (SC) injection one dose adding oregano essential oil in drinking water 1 day post-vaccination for 3 days. Group D: vaccinated with inactivated ND vaccine at 5-day-old via SC injection one dose only without oregano essential oil. Group E: nonvaccinated as the control group. Group F: non-vaccinated and adding oregano essential oil for 3 days in drinking water as the control group. The effect of oregano essential oil in drinking water combined with two forms of live and killed ND virus vaccines on the immune responses and serum proteins was investigated in broiler chickens. The results showed the significant effect of oregano on the immune response of chicks in groups D and C that were vaccinated with the killed vaccine at 23- and 33-day-old. Based on the findings, a peak was recorded at 38- day-old with a significant difference (P<0.05) in vaccinated and non-vaccinated groups, as well as a significant increase in 38-day-old for group C (P<0.05), compared to other groups regarding the oregano effects on the immune response of groups. In addition, an increase in the serum protein was significantly different (P>0.05) in the vaccinated broiler chicks, and serum globulin showed a quantitative increase in the vaccinated broiler chicks, compared with non-vaccinated groups. However, the results showed that the effect of oregano was nonsignificantly different (P>0.05) and it had no beneficial effect on growth performance. The results of this study showed the effects of commercial Oregano supplementation (50 mL/1000L drinking water), which increased serum total protein, including globulin, and provoked the best immunological response to ND vaccinations in broiler chickens. More research is needed to develop a state that may beneficially affect the health and uniformity of chickens. [ABSTRACT FROM AUTHOR]

It is well documented that choline is known as one of the essential ingredients of phospholipids. Choline acts as a determinative element for appropriate cell membrane functions. On the other hand a-tocopherol (Vit E) is a fat-soluble vitamin. This vitamin acts as a strong antioxidant in the living body's defense system against oxidative stress. Lipid peroxidation in peripartum and early lactating cows is significantly increased while the level of serum Vit E is decreases dramatically. These concomitant physiological changes demonstrate a higher level of oxidative stress subsequently leads to serious health issues in dairy cows. Therefore, the present research was designed to investigate the following items in dairy cattle: 1) evaluation of the possible changes in serum protein fractions, and 2) comparing the oxidative status of orally RPC and vitamin E supplementation in dairy cows in early lactation period. In the current study 30 early lactating primiparous and multiparous Holstein cows (body condition score (BCS)=2.51 ± 0.10) were used beginning five weeks postpartum. All the animals were randomly divided in to three groups (n=10) (number of lactation=2.61). The animals were randomly assigned to receive one of the following treatments. Group 1 served as control group were not received any supplement. The second group was supplemented with 90 g/d of RPC (Reashre Choline, Balchem, USA). The third group was administrated 4400 IU/d vitamin E (Roche, Vitamins Ltd; Switzerland). In the current study, serum protein electrophoresis showed four main fractions as follows: albumin, a-globulin, ß-globulin, and γ-globulin. The recorded data showed that the percentages of albumin and γ-globulin fractions were higher in treated groups compared to the control group. In the animals supplementing with RPC and vitamin E the percentages of serum albumin increased to the value of 37. 70±1.63 and 38.21±1.28 respectively compare to the control group (34.69±1.21), which were significant (P<0.05). [ABSTRACT FROM AUTHOR]

Chronic fluorosis results from long-term fluoride intake at more than the normal doses. The aim of this study was to demonstrate the effects of resveratrol (Res) on the serum protein fractions in rats, in which experimental chronic fluorosis was induced. After an adaptation period, the rats were randomly divided into 4 groups of 10; namely the (i) control, (ii) sodium fluoride (NaF), (iii) Res, and (iv) NaF+Res groups. Serum protein fractions in the rat blood samples were determined by cellulose-acetate electrophoresis. While the NaF group had statistically reduced concentrations of total protein, albumin, and a-1 and a-2 globulin compared to the control group (p<0.05), these values were significantly increased (p<0.05) in the NaF+Res group, compared to the NaF group, and close to those of the control group. The β- and γ-globulin concentrations were the lowest in the NaF group statistically (p<0.05). Despite a significant increase (p<0.05) in these values in the NaF+Res group, compared to the NaF group, they were still lower compared to the control group. The examination of the percentage of serum protein fractions revealed a reduced albumin in the NaF group compared to the control group but the finding was not statistically significant (p>0.05). The albumin of the NaF+Res group was statistically higher than that of the control group (p<0.05). No statistical differences were observed in α-1 and α-2 globulin across the groups. The β-globulin of the NaF group was the highest but not statistically higher than that of the control group. The γ-globulin percentages in all the groups were found to be lower than the levels in the control group. The albumin/globulin (A/G) ratio decreased in the NaF group but was not significantly different than that of the control group. In conclusion, the alterations in the serum protein fractions due to NaF-induced toxicity, especially the alterations in their concentrations, approached values closer to those of the control group with the administration of resveratrol. We concluded that these results are of potential importance in indicating a favorable role for resveratrol use in preventing and treating fluoride toxicity. [ABSTRACT FROM AUTHOR]

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Abstract Background Response to modern treatment strategies, which combine cytotoxic compounds with immune stimulatory agents and targeted treatment is highly variable among MCL patients. Thus, providing prognostic and predictive markers for risk adapted therapy is warranted and molecular information that can help in patient stratification is a necessity. In relapsed MCL, biopsies are rarely available and molecular information from tumor tissue is often lacking. Today, the main tool to access risk is the MCL international prognostic index (MIPI), which does not include detailed biological information of relevance for different treatment options. To enable continuous monitoring of patients, non-invasive companion diagnostic tools are needed which can further reduce cost and patient distress and enable efficient measurements of biological markers. Methods We have assessed if serum-based protein profiling can identify immune related proteins that stratify relapsed MCL patients based on risk. Overall, 371 scFv targeting 158 proteins were assessed using an antibody microarray platform. We profiled patients (n = 44) who had been treated within the MCL6-Philemon trial combining targeted and immune-modulatory treatment. Results The downstream processing led to the identification of the relapsed immune signature (RIS) consisting of 11 proteins with potential to stratify patients with long and short overall survival (OS). Moreover, in this population, MIPI alone failed to separate high, intermediate and low risk patients, but a combined index based on MIPI together with RIS, MIPIris, showed improved performance and significantly stratified all three risk groups based on OS. Conclusions Our results show that addition of biological parameters to previous prognostic indices improves patient stratification among patients treated with BTK inhibitor triplet combination, particularly, in the identification of an extreme high risk group.