1. A multi-valent polymyxin-based fluorescent probe for the detection of Gram-negative infections.
- Author
-
Sharma R, Rodriguez-Rios M, Crossland J, Septiyana M, Megia-Fernandez A, Klausen M, and Bradley M
- Subjects
- Gram-Negative Bacterial Infections drug therapy, Gram-Negative Bacterial Infections diagnosis, Gram-Negative Bacterial Infections microbiology, Humans, Molecular Structure, Oxadiazoles, Fluorescent Dyes chemistry, Fluorescent Dyes chemical synthesis, Polymyxins chemistry, Gram-Negative Bacteria isolation & purification
- Abstract
A multi-branched fluorogenic probe for the rapid and specific detection of Gram-negative bacteria is reported. Three Gram-negative-targeting azido-modified polymyxins were clicked onto a trivalent scaffold functionalised with the environmental green-emitting fluorophore 7-nitrobenz-2-oxa-1,3-diazole. The probe allowed wash-free detection of target bacteria with increased sensitivity and lower limits of detection compared to monovalent probes.
- Published
- 2025
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