Search

Your search keyword '"Seong, Mincheol"' showing total 236 results
Start Over Author "Seong, Mincheol"
236 results on '"Seong, Mincheol"'

3. MicroRNA Regulation for Inflammasomes in High Glucose‐Treated ARPE‐19 Cells.

Author

Kim, Ji Hong, Yu, Hyoseon, Kang, Ji Hye, Hong, Eun Hee, Kang, Min Ho, Seong, Mincheol, Cho, Heeyoon, Shin, Yong Un, and Honda, Shigeru

Subjects
PROTEINS, GLUCOSE, EPITHELIAL cells, RESEARCH funding, MICRORNA, DIABETIC retinopathy, POLYMERASE chain reaction, GENE expression, MICE, ANIMAL experimentation, WESTERN immunoblotting, RETINA
Abstract

Purpose. This study aimed to evaluate the expression of microRNAs (miRNAs) and inflammasomes in diabetes‐induced retinal cells and to determine their role in the pathogenesis of diabetic retinopathy (DR). Methods. To establish diabetes‐induced cell models, ARPE‐19 cells were treated with high glucose. The expression levels of five miRNAs (miR‐185, miR‐17, miR‐20a, miR‐15a, and miR‐15b) were measured in high glucose‐treated ARPE‐19 cells using real‐time quantitative polymerase chain reaction. Western blotting was performed to measure inflammasome expression in cellular models. miR‐17 was selected as the target miRNA, and inflammasome expression was measured following the transfection of an miR‐17 mimic into high glucose‐treated ARPE‐19 cells. Results. In high glucose‐treated ARPE‐19 cells, miRNA expression was substantially downregulated, whereas that of inflammasome components was significantly increased. Following the transfection of the miR‐17 mimic into high glucose‐treated ARPE‐19 cells, the levels of inflammasome components were significantly decreased. Conclusions. This study investigated the relationship between miRNAs and inflammasomes in diabetes‐induced cells using high glucose‐treated ARPE‐19 cells. These findings suggested that miR‐17 suppresses inflammasomes, thereby reducing the subsequent inflammatory response and indicating that miRNAs and inflammasomes could serve as new therapeutic targets for DR. [ABSTRACT FROM AUTHOR]

Published
2024
Full Text
View/download PDF

27. Wide-field optical coherence tomography deviation map for early glaucoma detection

Author

Kim, Hyungjun, Park, Hae Min, Jeong, Hyo Chan, Moon, So Yeon, Cho, Hyunsoo, Lim, Han Woong, Seong, Mincheol, Park, Junhong, and Lee, Won June

Abstract

Background/aimsThis study aimed to establish a wide-field optical coherence tomography (OCT) deviation map obtained from swept-source OCT (SS-OCT) scans. Moreover, it also aimed to compare the diagnostic ability of this wide-field deviation map with that of the peripapillary and macular deviation maps currently being used for the detection of early glaucoma (EG).MethodsFour hundred eyes, including 200 healthy eyes and 200 eyes with EG were enrolled in this retrospective observational study. Patients underwent a comprehensive ocular examination, including wide-field SS-OCT (DRI-OCT Triton; Topcon, Tokyo, Japan). The individual wide-field scan was converted into a uniform template using the fovea and optic disc centres as fixed landmarks. Subsequently, the wide-field deviation map was obtained via the comparison between individual wide-field data and a normative wide-field database that had been created by combining images of healthy eyes into a uniform template in a previous study. The ability of the new wide-field deviation map to distinguish between EG and healthy eyes was assessed by comparing it with conventional deviation maps based on the area under the receiver operating characteristic curve (AUC).ResultsThe wide-field deviation map obtained using the normative wide-field database showed the highest diagnostic ability for the diagnosis of EG (AUC=0.980 and 961 for colour-coded pixels presenting <5% and <1%, respectively) among various deviation maps. Its AUC was significantly superior to that of most conventional deviation maps (p<0.05). The wide-field deviation map demonstrated early structural glaucomatous damage well over a wider area.ConclusionThe wide-field SS-OCT deviation map exhibited good performance for distinguishing between eyes with EG and healthy eyes. The visualisation of the wider damaged area on the wide-field deviation map could be useful for the diagnosis of EG in clinical settings.

Published
2023
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results