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The histopathological subtyping and grading of oral squamous cell carcinoma are well established in human medicine, but there are currently no standard classification and grading schema used for oral squamous cell carcinoma in cats. In this study, it was aimed to investigate different subtypes and grades of feline oral squamous cell carcinoma and the possible role of the papillomavirus infection in the progression of this tumor by using the methods of histopathology, immunohistochemistry, and transmission electron microscopy. Out of the 32 tissue samples, conventional (14/31; 43.75%), verrucous (8/32; 25%), papillary (5/32; 15.62%), acantholytic (3/32; 9.37%), and adenosquamous (2/32; 6.25%) subtypes of the tumor were determined in the affected cats. Grading of the tumor was performed according to Anneroth's and Bryne's systems and revealed that there was no relationship between histopathological subtypes and grades of the cases. Histopathologic findings suggestive of papillomavirus infection were determined not only in verrucous and papillary subtypes as seen in human medicine, but also in different other subtypes of the tumor. Moreover, immunopositivity for papillomavirus was obtained from the conventional, acantholytic, and adenosquamous subtypes of the tumor. In conclusion, the relationship between grading and subtypes of oral squamous cell carcinoma observed in this study showed differences in comparison with the human counterparts. The effect of papillomavirus infection in the progression of oral squamous cell carcinoma remained uncertain. [ABSTRACT FROM AUTHOR]

Background/aim: New compounds for cancer treatment are needed due to persistenly unsatisfactory management of cancer. [PdCl(terpy)](sac)·2H2O] (sac=saccharinate, and terpy=2,2':6',2"-terpyridine) is a compound synthesized for this purpose. We investigated its anti-proliferative and pro-apoptotic effects on Ehrlich Ascites Carcinoma (EAC) in vivo., Materials and Methods: 42 Balb-c female mice were subcutaneously (s.c.) injected with EAC cells (1st day) and then randomly divided into 5 groups: control (0.9% NaCl), complex (2 mg/kg), complex (3 mg/kg) cisplatin (4 mg/kg) and paclitaxel (12.5 mg/kg). On the 5th and 12th day animals were drug administrated. At 14th day, animals were sacrificed. Expression of cell death and/or cell cycle-related markers (Bcl-2, Bax, active caspase-3, p53, PCNA) and apoptosis were investigated immunohisto-chemically. Survival-related markers (Akt, GSK-3β, IGF-1R, IR, IRS-1, p70S6K, PRAS40) were evaluated by luminex analysis., Results: Expression of p53, PCNA, Bcl-2 was found decreased (p<0.001) and that of active caspase-3, Bax, and apoptotic cells was found increased (p<0.001) in all groups. The survival-related markers did not show any statistical difference in complex groups., Conclusion: The Pd(II)-complex seems to have a strong anticancer activity on EAC by inducing apoptosis via both suppression of proliferation and activation of apoptosis in vivo, similar to the effects of cisplatin and paclitaxel., (Copyright © 2016 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved.)

Background/aim: [Pd(sac)(terpy)](sac)•4H2O (sac=saccharinate and terpy=2,2':6',2"-terpyridine) is newly-synthesized palladium(II) (Pd) complex. We investigated the antiproliferative and apoptotic effects of this complex on Ehrlich ascites carcinoma (EAC)., Materials and Methods: EAC cells were administered to 33 Balb/c mice. Mice were divided randomly into four groups: control, cisplatin, Pd(II) complex and paclitaxel. Control group animals received 0.9% NaCl; other groups received treatments cisplatin, Pd(II) complex and paclitaxel on days 7 and 12. At day 14, animals were sacrificed. Expression of active caspase-3, p53 and proliferating cell nuclear antigen (PCNA) was investigated and apoptosis was evaluated by terminal deoxynucleotidyltransferase (TdT)-mediated nick-end labelling (TUNEL) technique., Results: Expression of p53 and PCNA were found to be decreased (p<0.0001), cells with active caspase-3 and TUNEL-positive cells were found to be increased (p<0.0001) in all treatment groups., Conclusion: Like cisplatin and paclitaxel, this Pd(II) complex has a strong anticancer activity against EAC by inducing apoptosis and suppressing proliferation in vivo., (Copyright© 2015 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved.)

A 5-year-old, sexually intact poodle bitch was presented with a 2-year history of inguinal mass. A tentative diagnosis of hydrometra/mucometra with inguinal herniation was made and ovariohysterectomy with hernia repair was performed. Both fluid-filled uterine horns, both broad ligaments, and the uterine body were observed to be herniated through the inguinal ring. On histopathology, marked edema and diffuse hemorrhage were diagnosed in the uterus.

The aim of the present study was to investigate abortion storms that occurred in the Marmara region of Turkey in 2008-2009 using a real-time PCR. Two aborted foetuses were necropsied and histo-pathological findings reported herein. Ten lungs, 3 brains and one nasal swab from 10 aborted foetuses, 6 nasal swabs and 3 vaginal swabs from aborting mares were included in this study. EHV-1 was isolated from the lung, liver and brain of 1 aborted foetus. EHV-1 DNA was detected in the lungs, livers and spleens of 2 necropsied foetuses and in 3 lungs from 10 foetuses submitted for diagnosis. A brain from one of the aborted foetuses was also positive for EHV-1 DNA. EHV-4 DNA was detected only in a nasal swab of one of the tested foetuses. Neither EHV-1 nor EHV-4 DNA was detected in the swabs of aborting mares. Sequence analysis of the glycoprotein B of the strains was performed and a phylogenetic tree was generated. The results indicated that 4 of the 5 Turkish EHV-1 strains (TR02, TR03, TR04 and TR05) clustered together; the fifth strain (TR01) was slightly removed from the group and clustered with other EHV-1 from various origins. Single nucleotide polyporphism (SNP in ORF30) associated with neuropathogenesis was not detected in any of the strains. At necropsy, sub-milier focal necrosis in the liver and spleen was observed. Microscopically, focal coagulation necrosis and marked eosinophilic intranuclear and intracytoplasmic inclusion bodies in the hepatocytes localised around the necrotic areas in the liver. Severe coagulation necrosis in white pulp of the spleen was also observed., (Copyright © 2012 Elsevier Ltd. All rights reserved.)

Background: A spider monkey with severe dyspnea was referred to our clinic., Methods and Results: Radiographs revealed an enlarged cardiac silhouette. Ventricular tachycardia and ST segment depression were also diagnosed after an ECG. These findings coupled with the postmortem examination confirmed dilatative cardiomyopathy., Conclusions: This case is worthy of presentation since dilatative cardiomyopathy has been rarely encountered in spider monkeys., (© 2012 John Wiley & Sons A/S.)

In this report, an ameloblastoma case, which has been occurred on the left maxilla of a 10 year-old, male, German shepherd, was described on the clinical and morphological features. The mass with the dimensions of 5 x 2.5 cm was removed totally by maxillectomy and examined by radiography, magnetic resonance (MR) and biopsy. The tumor was histopathologically classified ameloblastoma of follicular type and in some fields showed acanthotic form. The invasiveness of the tumor was also observed to be high. This report is the first case of maxillary ameloblastoma in dogs in Turkey.