Environmental Citizenship has been one of the leading concepts of pro-environmental actions and consciousness of ordinary people in their everyday lives, as well as at stakeholders responsible for bringing decisions that have a direct or indirect impact on the environment. It has emerged along with other adjectival citizenship since the resurgence of interest in citizenship theory (Bell, 2006) and the increasing preoccupation with environmental sustainability and the injection of green values into political analyses (Cao, 2015). The concepts of Environmental Citizenship and Education for Environmental Citizenship are quite new and underutilized in Croatia, although some postulates of it have been included in school and university courses, particularly related in geography, biology, environmental science etc. However, educational system in Croatia still lacks environmental literacy and concern on the environment. Therefore, this study aims to investigate if the Environmental Citizenship has been adopted amongst students of key scientific disciplines that in the future will bring environmental- related decisions on the local, regional and national level, as well as educate future generations of pupils and students as Environmental Citizens. Adoption of the Environmental Citizenship has been investigated using the longitudinal questionnaire survey on the majority of senior undergraduate and master students of Geography and Environmental Science at the University of Zagreb. These disciplines have been selected as most likely to have transferred the knowledge and principles of the Environmental Citizenship. These cohorts also represent future stakeholders in urban and regional planning, tourism development, environmental protection and related areas and will be able to act as drivers of change towards the environment. Some of them will also teach geography in schools and will be able to transfer the knowledge and values of the Environmental Citizenship to elementary pupils and high school students. The questionnaire survey included a set of open-ended and forced- choice answers (mostly multiple answers and the Likert scale) related to their knowledge on the Environmental Citizenship, their knowledge and perception of environmental rights and duties (focusing mostly on Croatia), as well as examples of positive and negative environmental practices. Beside general questions on Environmental Citizenship, the questionnaire survey investigated in more detail the students’ perception and their practices related to Environmental Citizenship in some key fields related to environment, particularly sustainable transport, tourism and heritage use and protection.