Search

Your search keyword '"Selfors, Laura M."' showing total 260 results
Start Over Author "Selfors, Laura M."
260 results on '"Selfors, Laura M."'

1. Brca1 haploinsufficiency promotes early tumor onset and epigenetic alterations in a mouse model of hereditary breast cancer

Author

Li, Carman Man-Chung, Cordes, Alyssa, Oliphant, Michael U. J., Quinn, S. Aidan, Thomas, Mayura, Selfors, Laura M., Silvestri, Francesca, Girnius, Nomeda, Rinaldi, Gianmarco, Zoeller, Jason J., Shapiro, Hana, Tsiobikas, Christina, Gupta, Kushali P., Pathania, Shailja, Regev, Aviv, Kadoch, Cigall, Muthuswamy, Senthil K., and Brugge, Joan S.

Published
2024
Full Text
View/download PDF

2. Glutathione synthesis in the mouse liver supports lipid abundance through NRF2 repression

Author

Asantewaa, Gloria, Tuttle, Emily T., Ward, Nathan P., Kang, Yun Pyo, Kim, Yumi, Kavanagh, Madeline E., Girnius, Nomeda, Chen, Ying, Rodriguez, Katherine, Hecht, Fabio, Zocchi, Marco, Smorodintsev-Schiller, Leonid, Scales, TashJaé Q., Taylor, Kira, Alimohammadi, Fatemeh, Duncan, Renae P., Sechrist, Zachary R., Agostini-Vulaj, Diana, Schafer, Xenia L., Chang, Hayley, Smith, Zachary R., O’Connor, Thomas N., Whelan, Sarah, Selfors, Laura M., Crowdis, Jett, Gray, G. Kenneth, Bronson, Roderick T., Brenner, Dirk, Rufini, Alessandro, Dirksen, Robert T., Hezel, Aram F., Huber, Aaron R., Munger, Joshua, Cravatt, Benjamin F., Vasiliou, Vasilis, Cole, Calvin L., DeNicola, Gina M., and Harris, Isaac S.

Published
2024
Full Text
View/download PDF

3. Author Correction: Brca1 haploinsufficiency promotes early tumor onset and epigenetic alterations in a mouse model of hereditary breast cancer

Author

Li, Carman Man-Chung, Cordes, Alyssa, Oliphant, Michael U. J., Quinn, S. Aidan, Thomas, Mayura, Selfors, Laura M., Silvestri, Francesca, Girnius, Nomeda, Rinaldi, Gianmarco, Zoeller, Jason J., Shapiro, Hana, Tsiobikas, Christina, Gupta, Kushali P., Pathania, Shailja, Regev, Aviv, Kadoch, Cigall, Muthuswamy, Senthil K., and Brugge, Joan S.

Published
2024
Full Text
View/download PDF

4. A human breast atlas integrating single-cell proteomics and transcriptomics

Author

Gray, G Kenneth, Li, Carman Man-Chung, Rosenbluth, Jennifer M, Selfors, Laura M, Girnius, Nomeda, Lin, Jia-Ren, Schackmann, Ron CJ, Goh, Walter L, Moore, Kaitlin, Shapiro, Hana K, Mei, Shaolin, D'Andrea, Kurt, Nathanson, Katherine L, Sorger, Peter K, Santagata, Sandro, Regev, Aviv, Garber, Judy E, Dillon, Deborah A, and Brugge, Joan S

Subjects
Biochemistry and Cell Biology, Biological Sciences, Breast Cancer, Cancer, Human Genome, Genetics, Aging, Biotechnology, Underpinning research, 1.1 Normal biological development and functioning, Aetiology, 2.1 Biological and endogenous factors, Animals, Breast, Breast Neoplasms, Female, Humans, Mammary Glands, Animal, Pregnancy, Proteomics, Transcriptome, BRCA1, BRCA2, CyTOF mass cytometry, aging, breast cancer, cell state plasticity, mammary biology, multi-omic single-cell atlas, multiplexed tissue staining, organoids, scRNA-Seq, Medical and Health Sciences, Developmental Biology, Biochemistry and cell biology
Abstract

The breast is a dynamic organ whose response to physiological and pathophysiological conditions alters its disease susceptibility, yet the specific effects of these clinical variables on cell state remain poorly annotated. We present a unified, high-resolution breast atlas by integrating single-cell RNA-seq, mass cytometry, and cyclic immunofluorescence, encompassing a myriad of states. We define cell subtypes within the alveolar, hormone-sensing, and basal epithelial lineages, delineating associations of several subtypes with cancer risk factors, including age, parity, and BRCA2 germline mutation. Of particular interest is a subset of alveolar cells termed basal-luminal (BL) cells, which exhibit poor transcriptional lineage fidelity, accumulate with age, and carry a gene signature associated with basal-like breast cancer. We further utilize a medium-depletion approach to identify molecular factors regulating cell-subtype proportion in organoids. Together, these data are a rich resource to elucidate diverse mammary cell states.

Published
2022

5. Clinical evaluation of BCL-2/XL levels pre- and post- HER2-targeted therapy

Author

Zoeller, Jason J, Press, Michael F, Selfors, Laura M, Dering, Judy, Slamon, Dennis J, Hurvitz, Sara A, and Brugge, Joan S

Subjects
Biomedical and Clinical Sciences, Clinical Sciences, Oncology and Carcinogenesis, Breast Cancer, Cancer, Adult, Aged, Antibodies, Monoclonal, Humanized, Antineoplastic Agents, Antineoplastic Combined Chemotherapy Protocols, Biomarkers, Tumor, Breast Neoplasms, Cell Proliferation, Female, Gene Expression Regulation, Neoplastic, Humans, Lapatinib, Middle Aged, Neoadjuvant Therapy, Protein Kinase Inhibitors, Proto-Oncogene Proteins c-bcl-2, Quinazolines, RNA, Messenger, Receptor, ErbB-2, Trastuzumab, Up-Regulation, Receptor, erbB-2, General Science & Technology
Abstract

Our previous pre-clinical work defined BCL-2 induction as a critical component of the adaptive response to lapatinib-mediated inhibition of HER2. To determine whether a similar BCL-2 upregulation occurs in lapatinib-treated patients, we evaluated gene expression within tumor biopsies, collected before and after lapatinib or trastuzumab treatment, from the TRIO-B-07 clinical trial (NCT#00769470). We detected BCL2 mRNA upregulation in both HER2+/ER- as well as HER2+/ER+ patient tumors treated with lapatinib or trastuzumab. To address whether mRNA expression correlated with protein expression, we evaluated pre- and post-treatment tumors for BCL-2 via immunohistochemistry. Despite BCL2 mRNA upregulation within HER2+/ER- tumors, BCL-2 protein levels were undetectable in most of the lapatinib- or trastuzumab-treated HER2+/ER- tumors. BCL-2 upregulation was evident within the majority of lapatinib-treated HER2+/ER+ tumors and was often coupled with increased ER expression and decreased proliferation. Comparable BCL-2 upregulation was not observed within the trastuzumab-treated HER2+/ER+ tumors. Together, these results provide clinical validation of the BCL-2 induction associated with the adaptive response to lapatinib and support evaluation of BCL-2 inhibitors within the context of lapatinib and other HER2-targeted receptor tyrosine kinase inhibitors.

Published
2021

13. Deubiquitinases Maintain Protein Homeostasis and Survival of Cancer Cells upon Glutathione Depletion

Author

Harris, Isaac S., Endress, Jennifer E., Coloff, Jonathan L., Selfors, Laura M., McBrayer, Samuel K., Rosenbluth, Jennifer M., Takahashi, Nobuaki, Dhakal, Sabin, Koduri, Vidyasagar, Oser, Matthew G., Schauer, Nathan J., Doherty, Laura M., Hong, Andrew L., Kang, Yun Pyo, Younger, Scott T., Doench, John G., Hahn, William C., Buhrlage, Sara J., DeNicola, Gina M., Kaelin, William G., Jr., and Brugge, Joan S.

Published
2019
Full Text
View/download PDF

14. Mesenchymal gene program–expressing ovarian cancer spheroids exhibit enhanced mesothelial clearance

Author

Davidowitz, Rachel A, Selfors, Laura M, Iwanicki, Marcin P, Elias, Kevin M, Karst, Alison, Piao, Huiying, Ince, Tan A, Drage, Michael G, Dering, Judy, Konecny, Gottfried E, Matulonis, Ursula, Mills, Gordon B, Slamon, Dennis J, Drapkin, Ronny, and Brugge, Joan S

Subjects
Cancer, Rare Diseases, Ovarian Cancer, Biotechnology, Genetics, Aetiology, 2.1 Biological and endogenous factors, Epithelial-Mesenchymal Transition, Epithelium, Female, Gene Knockdown Techniques, Homeodomain Proteins, Humans, Mesoderm, Neoplasm Invasiveness, Nuclear Proteins, Ovarian Neoplasms, Peritoneal Neoplasms, Protein Array Analysis, Snail Family Transcription Factors, Spheroids, Cellular, Transcription Factors, Transcriptome, Tumor Cells, Cultured, Twist-Related Protein 1, Zinc Finger E-box-Binding Homeobox 1, Medical and Health Sciences, Immunology
Abstract

Metastatic dissemination of ovarian tumors involves the invasion of tumor cell clusters into the mesothelial cell lining of peritoneal cavity organs; however, the tumor-specific factors that allow ovarian cancer cells to spread are unclear. We used an in vitro assay that models the initial step of ovarian cancer metastasis, clearance of the mesothelial cell layer, to examine the clearance ability of a large panel of both established and primary ovarian tumor cells. Comparison of the gene and protein expression profiles of clearance-competent and clearance-incompetent cells revealed that mesenchymal genes are enriched in tumor populations that display strong clearance activity, while epithelial genes are enriched in those with weak or undetectable activity. Overexpression of transcription factors SNAI1, TWIST1, and ZEB1, which regulate the epithelial-to-mesenchymal transition (EMT), promoted mesothelial clearance in cell lines with weak activity, while knockdown of the EMT-regulatory transcription factors TWIST1 and ZEB1 attenuated mesothelial clearance in ovarian cancer cell lines with strong activity. These findings provide important insights into the mechanisms associated with metastatic progression of ovarian cancer and suggest that inhibiting pathways that drive mesenchymal programs may suppress tumor cell invasion of peritoneal tissues.

Published
2014

17. Transient commensal clonal interactions can drive tumor metastasis

Author

Naffar-Abu Amara, Suha, Kuiken, Hendrik J., Selfors, Laura M., Butler, Timothy, Leung, Marco L., Leung, Cheuk T., Kuhn, Elaine P., Kolarova, Teodora, Hage, Carina, Ganesh, Kripa, Panayiotou, Richard, Foster, Rosemary, Rueda, Bo R., Aktipis, Athena, Spellman, Paul, Ince, Tan A., Xiu, Joanne, Oberley, Matthew, Gatalica, Zoran, Navin, Nicholas, Mills, Gordon B., Bronson, Rodrick T., and Brugge, Joan S.

Published
2020
Full Text
View/download PDF

20. Supplementary Figure S5 from Combined MEK and BCL-2/XL Inhibition Is Effective in High-Grade Serous Ovarian Cancer Patient–Derived Xenograft Models and BIM Levels Are Predictive of Responsiveness

Author

Iavarone, Claudia, primary, Zervantonakis, Ioannis K., primary, Selfors, Laura M., primary, Palakurthi, Sangeetha, primary, Liu, Joyce F., primary, Drapkin, Ronny, primary, Matulonis, Ursula A., primary, Hallberg, Dorothy, primary, Velculescu, Victor E., primary, Leverson, Joel D., primary, Sampath, Deepak, primary, Mills, Gordon B., primary, and Brugge, Joan S., primary

Published
2023
Full Text
View/download PDF

21. Data from Combined MEK and BCL-2/XL Inhibition Is Effective in High-Grade Serous Ovarian Cancer Patient–Derived Xenograft Models and BIM Levels Are Predictive of Responsiveness

Author

Iavarone, Claudia, primary, Zervantonakis, Ioannis K., primary, Selfors, Laura M., primary, Palakurthi, Sangeetha, primary, Liu, Joyce F., primary, Drapkin, Ronny, primary, Matulonis, Ursula A., primary, Hallberg, Dorothy, primary, Velculescu, Victor E., primary, Leverson, Joel D., primary, Sampath, Deepak, primary, Mills, Gordon B., primary, and Brugge, Joan S., primary

Published
2023
Full Text
View/download PDF

22. Supplementary Table 1 from Combined MEK and BCL-2/XL Inhibition Is Effective in High-Grade Serous Ovarian Cancer Patient–Derived Xenograft Models and BIM Levels Are Predictive of Responsiveness

Author

Iavarone, Claudia, primary, Zervantonakis, Ioannis K., primary, Selfors, Laura M., primary, Palakurthi, Sangeetha, primary, Liu, Joyce F., primary, Drapkin, Ronny, primary, Matulonis, Ursula A., primary, Hallberg, Dorothy, primary, Velculescu, Victor E., primary, Leverson, Joel D., primary, Sampath, Deepak, primary, Mills, Gordon B., primary, and Brugge, Joan S., primary

Published
2023
Full Text
View/download PDF

27. Supplemental Table 2 from Establishment of Patient-Derived Tumor Xenograft Models of Epithelial Ovarian Cancer for Preclinical Evaluation of Novel Therapeutics

Author

Liu, Joyce F., primary, Palakurthi, Sangeetha, primary, Zeng, Qing, primary, Zhou, Shan, primary, Ivanova, Elena, primary, Huang, Wei, primary, Zervantonakis, Ioannis K., primary, Selfors, Laura M., primary, Shen, Yiping, primary, Pritchard, Colin C., primary, Zheng, Mei, primary, Adleff, Vilmos, primary, Papp, Eniko, primary, Piao, Huiying, primary, Novak, Marian, primary, Fotheringham, Susan, primary, Wulf, Gerburg M., primary, English, Jessie, primary, Kirschmeier, Paul T., primary, Velculescu, Victor E., primary, Paweletz, Cloud, primary, Mills, Gordon B., primary, Livingston, David M., primary, Brugge, Joan S., primary, Matulonis, Ursula A., primary, and Drapkin, Ronny, primary

Published
2023
Full Text
View/download PDF

29. Supplemental Tables 1, 3, and 6; Supplemental Figures 1-5 from Establishment of Patient-Derived Tumor Xenograft Models of Epithelial Ovarian Cancer for Preclinical Evaluation of Novel Therapeutics

Author

Liu, Joyce F., primary, Palakurthi, Sangeetha, primary, Zeng, Qing, primary, Zhou, Shan, primary, Ivanova, Elena, primary, Huang, Wei, primary, Zervantonakis, Ioannis K., primary, Selfors, Laura M., primary, Shen, Yiping, primary, Pritchard, Colin C., primary, Zheng, Mei, primary, Adleff, Vilmos, primary, Papp, Eniko, primary, Piao, Huiying, primary, Novak, Marian, primary, Fotheringham, Susan, primary, Wulf, Gerburg M., primary, English, Jessie, primary, Kirschmeier, Paul T., primary, Velculescu, Victor E., primary, Paweletz, Cloud, primary, Mills, Gordon B., primary, Livingston, David M., primary, Brugge, Joan S., primary, Matulonis, Ursula A., primary, and Drapkin, Ronny, primary

Published
2023
Full Text
View/download PDF

31. Supplemental Table 5 from Establishment of Patient-Derived Tumor Xenograft Models of Epithelial Ovarian Cancer for Preclinical Evaluation of Novel Therapeutics

Author

Liu, Joyce F., primary, Palakurthi, Sangeetha, primary, Zeng, Qing, primary, Zhou, Shan, primary, Ivanova, Elena, primary, Huang, Wei, primary, Zervantonakis, Ioannis K., primary, Selfors, Laura M., primary, Shen, Yiping, primary, Pritchard, Colin C., primary, Zheng, Mei, primary, Adleff, Vilmos, primary, Papp, Eniko, primary, Piao, Huiying, primary, Novak, Marian, primary, Fotheringham, Susan, primary, Wulf, Gerburg M., primary, English, Jessie, primary, Kirschmeier, Paul T., primary, Velculescu, Victor E., primary, Paweletz, Cloud, primary, Mills, Gordon B., primary, Livingston, David M., primary, Brugge, Joan S., primary, Matulonis, Ursula A., primary, and Drapkin, Ronny, primary

Published
2023
Full Text
View/download PDF

38. Abstract PD5-02: PD5-02 An Organoid Model System to Study Resistance Mechanisms, Predictive Biomarkers, and New Strategies to Overcome Therapeutic Resistance in Early-Stage Triple-Negative Breast Cancer

Author

Bui, Tam Binh V., primary, Wolf, Denise M., additional, Moore, Kaitlin, additional, Harris, Isaac S., additional, Phadatare, Pravin, additional, Yau, Christina, additional, Swigart, Lamorna A. Brown, additional, Esserman, Laura J., additional, Coppe, Jean-Philippe, additional, Wulfkuhle, Julia, additional, Petricoin, Emanuel F., additional, Campbell, Michael, additional, Selfors, Laura M., additional, Dillon, Deborah A., additional, Overmoyer, Beth, additional, Lynce, Filipa, additional, Van ’t Veer, Laura, additional, and Rosenbluth, Jennifer, additional

Published
2023
Full Text
View/download PDF

39. Glutathione supports lipid abundancein vivo

Author

Asantewaa, Gloria, primary, Tuttle, Emily T., additional, Ward, Nathan P., additional, Kang, Yun Pyo, additional, Kim, Yumi, additional, Kavanagh, Madeline E., additional, Girnius, Nomeda, additional, Chen, Ying, additional, Duncan, Renae, additional, Rodriguez, Katherine, additional, Hecht, Fabio, additional, Zocchi, Marco, additional, Smorodintsev-Schiller, Leonid, additional, Scales, TashJaé Q., additional, Taylor, Kira, additional, Alimohammadi, Fatemeh, additional, Sechrist, Zachary R, additional, Agostini-Vulaj, Diana, additional, Schafer, Xenia L., additional, Chang, Hayley, additional, Smith, Zachary, additional, O’Connor, Thomas N., additional, Whelan, Sarah, additional, Selfors, Laura M., additional, Crowdis, Jett, additional, Gray, G. Kenneth, additional, Bronson, Roderick T., additional, Brenner, Dirk, additional, Rufini, Alessandro, additional, Dirksen, Robert T., additional, Hezel, Aram F., additional, Huber, Aaron R., additional, Munger, Josh, additional, Cravatt, Benjamin F., additional, Vasiliou, Vasilis, additional, Cole, Calvin L, additional, DeNicola, Gina M., additional, and Harris, Isaac S., additional

Published
2023
Full Text
View/download PDF

40. Brca1haploinsufficiency promotes early tumor onset and epigenetic alterations in a mouse model of hereditary breast cancer

Author

Li, Carman Man-Chung, Cordes, Alyssa, Oliphant, Michael U. J., Quinn, S. Aidan, Thomas, Mayura, Selfors, Laura M., Silvestri, Francesca, Girnius, Nomeda, Rinaldi, Gianmarco, Zoeller, Jason J., Shapiro, Hana, Tsiobikas, Christina, Gupta, Kushali P., Pathania, Shailja, Regev, Aviv, Kadoch, S. Cigall, Muthuswamy, Senthil K., and Brugge, Joan S.

Abstract

Germline BRCA1mutation carriers face a high breast cancer risk; however, the underlying mechanisms for this risk are not completely understood. Using a new genetically engineered mouse model of germline Brca1heterozygosity, we demonstrate that early tumor onset in a Brca1heterozygous background cannot be fully explained by the conventional ‘two-hit’ hypothesis, suggesting the existence of inherent tumor-promoting alterations in the Brca1heterozygous state. Single-cell RNA sequencing and assay for transposase-accessible chromatin with sequencing analyses uncover a unique set of differentially accessible chromatin regions in ostensibly normal Brca1heterozygous mammary epithelial cells, distinct from wild-type cells and partially mimicking the chromatin and RNA-level changes in tumor cells. Transcription factor analyses identify loss of ELF5 and gain of AP-1 sites in these epigenetically primed regions; in vivo experiments further implicate AP-1 and Wnt10aas strong promoters of Brca1-related breast cancer. These findings reveal a previously unappreciated epigenetic effect of Brca1haploinsufficiency in accelerating tumorigenesis, advancing our mechanistic understanding and informing potential therapeutic strategies.

Published
2024
Full Text
View/download PDF

47. Clonal populations of a human TNBC model display significant functional heterogeneity and divergent growth dynamics in distinct contexts

Author

Kuiken, Hendrik J., primary, Dhakal, Sabin, additional, Selfors, Laura M., additional, Friend, Chandler M., additional, Zhang, Tian, additional, Callari, Maurizio, additional, Schackmann, Ron C. J., additional, Gray, G. Kenneth, additional, Crowdis, Jett, additional, Bhang, Hyo-eun C., additional, Baslan, Timour, additional, Stegmeier, Frank, additional, Gygi, Steven P., additional, Caldas, Carlos, additional, and Brugge, Joan S., additional

Published
2021
Full Text
View/download PDF

48. Aging-Associated Alterations in Mammary Epithelia and Stroma Revealed by Single-Cell RNA Sequencing

Author

Massachusetts Institute of Technology. Department of Biology, Li, Carman Man-Chung, Shapiro, Hana, Tsiobikas, Christina, Selfors, Laura M, Chen, Huidong, Rosenbluth, Jennifer, Moore, Kaitlin, Gupta, Kushali P, Gray, G Kenneth, Oren, Yaara, Steinbaugh, Michael J, Guerriero, Jennifer L, Pinello, Luca, Regev, Aviv, Brugge, Joan S, Massachusetts Institute of Technology. Department of Biology, Li, Carman Man-Chung, Shapiro, Hana, Tsiobikas, Christina, Selfors, Laura M, Chen, Huidong, Rosenbluth, Jennifer, Moore, Kaitlin, Gupta, Kushali P, Gray, G Kenneth, Oren, Yaara, Steinbaugh, Michael J, Guerriero, Jennifer L, Pinello, Luca, Regev, Aviv, and Brugge, Joan S

Abstract

© 2020 The Author(s) Aging is closely associated with increased susceptibility to breast cancer, yet there have been limited systematic studies of aging-induced alterations in the mammary gland. Here, we leverage high-throughput single-cell RNA sequencing to generate a detailed transcriptomic atlas of young and aged murine mammary tissues. By analyzing epithelial, stromal, and immune cells, we identify age-dependent alterations in cell proportions and gene expression, providing evidence that suggests alveolar maturation and physiological decline. The analysis also uncovers potential pro-tumorigenic mechanisms coupled to the age-associated loss of tumor suppressor function and change in microenvironment. In addition, we identify a rare, age-dependent luminal population co-expressing hormone-sensing and secretory-alveolar lineage markers, as well as two macrophage populations expressing distinct gene signatures, underscoring the complex heterogeneity of the mammary epithelia and stroma. Collectively, this rich single-cell atlas reveals the effects of aging on mammary physiology and can serve as a useful resource for understanding aging-associated cancer risk.

Published
2021

49. Abstract PD7-03: A living biobank of normal mammary organoids derived from patients at low and increased risk of developing breast cancer

Author

Rosenbluth, Jennifer M, primary, Li, Carman Man-Chung, additional, Gray, Kenneth G, additional, Gao, Walter, additional, Schackmann, Ron CJ, additional, Zoeller, Jason J, additional, Selfors, Laura M, additional, Moore, Kaitlin, additional, Dillon, Deborah, additional, Garber, Judy, additional, and Brugge, Joan S, additional

Published
2021
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results