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Much research has examined the association between precarious employment and wellbeing in adults, but little is known about this relationship in working students. Using a sample of 224 (M[subscript Age] 21 years; 68% female), we assessed self-perceptions of job precariousness across four domains (i.e., job insecurity, remuneration, conditions, flexibility) and tested the relationships between the four domains and student burnout, and whether these relationships could be explained sequentially by higher levels of job and financial strain and sleep disruption. Job insecurity alone related both directly and indirectly to burnout (via job and financial strain and poor sleep quality). Precariousness related to financial strain (insecurity, remuneration), job strain (insecurity, flexibility), and sleep quality (insecurity); financial and job strain related to sleep quality; and sleep quality related to burnout. By decomposing the job precariousness construct, the findings provide an improved understanding of how working in low quality, precarious jobs is related to student wellbeing.

Purpose: This study illuminates the assumption that internships facilitate labor market entry and answers the question of why internships have a positive effect on students' self-perceived employability. It is assumed that internships enable more positive employability perceptions by reducing career-entry worries -- the worries of not finding a suitable job or not being able to obtain a satisfactory career. Design/methodology/approach: A two-wave study among graduate students currently in an internship investigated these relationships. Data on career-entry worries, perceived employability and an evaluation of the internship were collected from 80 students (mean age: 24.6 years, 68% female) from various fields of study aiming at both bachelor's and master's degrees. Findings: The results showed that positively evaluated internships contributed to graduates' self-perceived employability by means of reduced career-entry worries over an eight-week period. Originality/value: By considering graduates' career-entry worries -- the perceived uncertainty about finding an "appropriate" career in the future -- the authors introduce a new concept to the career literature and show that these worries are significant in terms of self-assessed employability.

The high rates of training transfer failure that prevail still puzzle practitioners as well as researchers. The central aim of the present study is to analyze the relatively under-researched role of job dissatisfaction in the training transfer process. Specifically, we expect that job dissatisfaction would have a negative effect on transfer but that this effect would be buffered by the expectation of positive transfer consequences and motivation to transfer. To test these hypotheses, 220 participants in different training programs completed an online questionnaire 1 year after training. The results support our assumptions. They reveal that job dissatisfaction has a detrimental effect on training transfer, but that motivation to transfer and the expectation of positive transfer consequences have a buffering effect. The more motivated a person is towards transfer, the less negative is the effect of job dissatisfaction on actual transfer, but only if a person expects positive outcomes from transfer, such as acknowledgment or rewards. The findings are discussed in relation to existing training transfer models, as well as models of job (dis)satisfaction.

Dental Stem Cells (DSCs) from discarded teeth are a non-invasive and ethically favorable source with the potential for neurogenesis due to their ectodermal origin. Stem cells from human exfoliated deciduous teeth (SHED) are particularly promising due to their high differentiation potential and relative immaturity compared to other Mesenchymal Stromal Cells (MSCs). Markers like CD56 and CD271 are critical in identifying MSC subpopulations for therapeutic applications because of their roles in neurodevelopment and maintaining stemness. This study investigates how fetal bovine serum (FBS) concentrations affect the expression of CD56 and CD271 in SHED, influencing their stemness and neuronal differentiation potential. SHEDs were isolated from various donors, cultured, and characterized for MSC traits using markers such as CD14, CD19, CD29, CD34, CD45, CD73, CD90, CD105, CD56, and CD271. Culturing SHED in different FBS conditions (standard 15 %, reduced 1 % and 5 %, and FBS-free) showed that lower FBS concentrations increase CD271 and CD56 expression while maintaining the standard MSC immunophenotype. Importantly, the enhanced expression of these markers can be induced even after SHEDs have been expanded in standard FBS concentrations. These findings suggest that FBS concentration can optimize SHED culture conditions, enhancing their suitability for regenerative medicine and tissue engineering applications. • SHED shows high neurogenic potential. • Lowering FBS increases CD271 and CD56 expression. • FBS concentration affects MSC characteristics. [ABSTRACT FROM AUTHOR]

Abstract While supervised work placements are increasingly popular in higher education, evidence regarding their effects on career outcomes remain somewhat sparse and atheoretical. The aim of this systematic literature review is to evaluate the effectiveness of placements for career outcomes and to identify any underpinning core psychological processes and to offer a theoretically grounded framework for future research. Drawing on transition theory (Schlossberg, 1981) and career construction theory (Savickas, 1997), we argue that supervised work experiences are central transition experiences that enable social learning processes and trigger changes in a person's identity development as a professional, thereby increasing career resources and employability which in turn affect future career outcomes positively. We screened 2394 systematically selected abstracts across several databases and disciplines. Only quantitative studies that either offered a control-group or a longitudinal design were included, resulting in an in-depth review of 40 studies, applying a rigorous evaluation protocol. Placement participation elicits an overall positive (but small) effect on career outcomes: Graduates who completed a work placement found employment more quickly. Work placements also changed students' perceptions of self-efficacy, their knowledge, skills, and attitudes. We suggest that these changes could be seen as indicative of the proposed social learning processes and identity changes that positively affect career resources. Our review points to several gaps in the literature, and building on existing career theories, we develop a theoretical model and offer new avenues for future research to integrate the heterogenic field of placement research and inform career research in other areas. Highlights • Work placements improve employment prospects, but evidence on subjective career outcomes is mixed. • Most existing studies on work placements lack theoretical basis and empirical rigor. • Placements can be viewed as career transition events which lead to identity construction in the work context. • Learning and identity change processes interact and can thereby explain individual and career related growth in placements. [ABSTRACT FROM AUTHOR]

The personality high-order concept of core self-evaluations (CSE), which refers to a basic evaluation of one's worth, capability, and effectiveness, has attracted a lot of research interest. Yet little is known about the construct validity of the core self-evaluation scale (CSES) while information on its longitudinal factorial validity is wholly lacking. This study investigated the factor structure of the CSES using both confirmatory and exploratory factor analysis implemented in Mplus program, in addition, the factor loading invariance over time was investigated using exploratory structural equation modeling. Longitudinal data with three follow-ups over 2 years, gathered among university employees (n = 926 (T3)-2,137 (T1)), were used. The results showed that a two-factor solution comprising the sub-dimensions Internal and External self-evaluations fitted to the data better than the alternative factor models. The two-factor solution was also invariant across the three measurements. It Is concluded that the CSES could be used as a two-dimensional instead of a one-dimensional scale. Splitting the scale into the two sub-dimensions of Internal and External self-evaluations revealed that the concept has a finer-grained structure than hitherto thought. [ABSTRACT FROM AUTHOR]

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The Parkinson's disease protein α-synuclein (αSyn) promotes membrane fusion and fission by interacting with various negatively charged phospholipids. Despite postulated roles in endocytosis and exocytosis, plasma membrane (PM) interactions of αSyn are poorly understood. Here, we show that phosphatidylinositol 4,5-bisphosphate (PIP 2 ) and phosphatidylinositol 3,4,5-trisphosphate (PIP 3 ), two highly acidic components of inner PM leaflets, mediate PM localization of endogenous pools of αSyn in A2780, HeLa, SK-MEL-2, and differentiated and undifferentiated neuronal SH-SY5Y cells. We demonstrate that αSyn binds to reconstituted PIP 2 membranes in a helical conformation in vitro and that PIP 2 synthesizing kinases and hydrolyzing phosphatases reversibly redistribute αSyn in cells. We further delineate that αSyn-PM targeting follows phosphoinositide-3 kinase (PI3K)-dependent changes of cellular PIP 2 and PIP 3 levels, which collectively suggests that phosphatidylinositol polyphosphates contribute to αSyn's function(s) at the plasma membrane., Competing Interests: RJ, CE, AD, DM, PS No competing interests declared, (© 2021, Jacob et al.)

Heterogeneous aggregates of the human protein α-synuclein (αSyn) are abundantly found in Lewy body inclusions of Parkinson's disease patients. While structural information on classical αSyn amyloid fibrils is available, little is known about the conformational properties of disease-relevant, non-canonical aggregates. Here, we analyze the structural and dynamic properties of megadalton-sized dityrosine adducts of αSyn that form in the presence of reactive oxygen species and cytochrome c, a proapoptotic peroxidase that is released from mitochondria during sustained oxidative stress. In contrast to canonical cross-β amyloids, these aggregates retain high degrees of internal dynamics, which enables their characterization by solution-state NMR spectroscopy. We find that intermolecular dityrosine crosslinks restrict αSyn motions only locally whereas large segments of concatenated molecules remain flexible and disordered. Indistinguishable aggregates form in crowded in vitro solutions and in complex environments of mammalian cell lysates, where relative amounts of free reactive oxygen species, rather than cytochrome c, are rate limiting. We further establish that dityrosine adducts inhibit classical amyloid formation by maintaining αSyn in its monomeric form and that they are non-cytotoxic despite retaining basic membrane-binding properties. Our results suggest that oxidative αSyn aggregation scavenges cytochrome c's activity into the formation of amorphous, high molecular-weight structures that may contribute to the structural diversity of Lewy body deposits., Competing Interests: Declaration of Competing Interest The authors declare that they have no competing interests., (Copyright © 2020 The Author(s). Published by Elsevier Ltd.. All rights reserved.)

Oxidation of protein methionines to methionine-sulfoxides (MetOx) is associated with several age-related diseases. In healthy cells, MetOx is reduced to methionine by two families of conserved methionine sulfoxide reductase enzymes, MSRA and MSRB that specifically target the S- or R-diastereoisomers of methionine-sulfoxides, respectively. To directly interrogate MSRA and MSRB functions in cellular settings, we developed an NMR-based biosensor that we call CarMetOx to simultaneously measure both enzyme activities in single reaction setups. We demonstrate the suitability of our strategy to delineate MSR functions in complex biological environments, including cell lysates and live zebrafish embryos. Thereby, we establish differences in substrate specificities between prokaryotic and eukaryotic MSRs and introduce CarMetOx as a highly sensitive tool for studying therapeutic targets of oxidative stress-related human diseases and redox regulated signaling pathways., (© 2020 Wiley-VCH GmbH.)

Cells continuously adapt cellular processes by integrating external and internal signals. In yeast, multiple stress signals regulate pheromone signaling to prevent mating under unfavorable conditions. However, the underlying crosstalk mechanisms remain poorly understood. Here, we show that mechanical stress activates Pkc1, which prevents lysis of pheromone-treated cells by inhibiting polarized growth. In vitro Pkc1 phosphorylates conserved residues within the RING-H2 domains of the scaffold proteins Far1 and Ste5, which are also phosphorylated in vivo. Interestingly, Pkc1 triggers dispersal of Ste5 from mating projections upon mechanically induced stress and during cell-cell fusion, leading to inhibition of the MAPK Fus3. Indeed, RING phosphorylation interferes with Ste5 membrane association by preventing binding to the receptor-linked Gβγ protein. Cells expressing nonphosphorylatable Ste5 undergo increased lysis upon mechanical stress and exhibit defects in cell-cell fusion during mating, which is exacerbated by simultaneous expression of nonphosphorylatable Far1. These results uncover a mechanical stress-triggered crosstalk mechanism modulating pheromone signaling, polarized growth, and cell-cell fusion during mating., (© 2019 van Drogen et al.)

Nuclear magnetic resonance (NMR) spectroscopy is a versatile tool for investigating cellular structures and their compositions. While in vivo and whole-cell NMR have a long tradition in cell-based approaches, high-resolution in-cell NMR spectroscopy is a new addition to these methods. In recent years, technological advancements in multiple areas provided converging benefits for cellular MR applications, especially in terms of robustness, reproducibility and physiological relevance. Here, we review the use of cellular NMR methods for drug discovery purposes in academia and industry. Specifically, we discuss how developments in NMR technologies such as miniaturized bioreactors and flow-probe perfusion systems have helped to consolidate NMR's role in cell-based drug discovery efforts., (Copyright © 2019 The Author(s). Published by Elsevier Inc. All rights reserved.)

Delivery of native or chemically modified recombinant proteins into mammalian cells shows promise for functional investigations and various technological applications, but concerns that sub-cellular localization and functional integrity of delivered proteins may be affected remain high. Here, we surveyed batch electroporation as a delivery tool for single polypeptides and multi-subunit protein assemblies of the kinetochore, a spatially confined and well-studied subcellular structure. After electroporation into human cells, recombinant fluorescent Ndc80 and Mis12 multi-subunit complexes exhibited native localization, physically interacted with endogenous binding partners, and functionally complemented depleted endogenous counterparts to promote mitotic checkpoint signaling and chromosome segregation. Farnesylation is required for kinetochore localization of the Dynein adaptor Spindly. In cells with chronically inhibited farnesyl transferase activity, in vitro farnesylation and electroporation of recombinant Spindly faithfully resulted in robust kinetochore localization. Our data show that electroporation is well-suited to deliver synthetic and chemically modified versions of functional proteins, and, therefore, constitutes a promising tool for applications in chemical and synthetic biology., Competing Interests: AA, VP, SP, MS, SV, GC, KO, BV, SW, AP, YW, PS, AM, SM No competing interests declared, (© 2019, Alex et al.)