148 results on '"Sejima T"'
Search Results
2. Post-fertilization effect of bilateral primary testicular damage induced by unilateral cryptorchidism in the rat model
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Tsounapi, P., Honda, M., Dimitriadis, F., Shimizu, S., Hikita, K., Muraoka, K., Sejima, T., Saito, M., Tomita, S., Sofikitis, N., and Takenaka, A.
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- 2016
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3. Classification of the distribution of cavernous nerve fibers around the prostate by intraoperative electrical stimulation during laparoscopic radical prostatectomy
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Takenaka, A, Soga, H, Hinata, N, Honda, M, Sejima, T, Muramaki, M, Miyake, H, Tanaka, K, and Fujisawa, M
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- 2011
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4. Blocking of the ATP sensitive potassium channel ameliorates the ischaemia-reperfusion injury in the rat testis
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Shimizu, S., Oikawa, R., Tsounapi, P., Inoue, K., Shimizu, T., Tanaka, K., Martin, D. T., Honda, M., Sejima, T., Tomita, S., and Saito, M.
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- 2014
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5. 1089 The urodynamic effects of intravesical administration of sensory neuron-specific receptor agonist on cyclophosphamide-induced bladder overactivity in rats
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Honda, M., Yoshimura, N., Kawamoto, B., Hikita, K., Muraoka, K., Panagiota, T., Shimizu, S., Saito, M., Sejima, T., Chancellor, M.B., and Takenaka, A.
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- 2015
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6. 732 The utility of histopathological evaluation in the nephrectomized non-neoplastic renal parenchyma for predicting life-threatening surgical chronic kidney disease in patients with renal cell carcinomas treated by radical nephrectomy
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Sejima, T., Yamaguchi, N., Iwamoto, H., Masago, T., Morizane, S., Honda, M., and Takenaka, A.
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- 2015
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7. 414 The utility of 3-dimensional image system of robotic surgery on the basis of a comprehensive department in academic institutional framework
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Masago, T., Yamaguchi, N., Iwamoto, H., Morizane, S., Honda, M., Sejima, T., and Takenaka, A.
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- 2015
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8. 358 A novel risk classification tool to estimate urinary incontinence after robotic-assisted radical prostatectomy
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Honda, M., Kawamoto, B., Morizane, S., Hikita, K., Muraoka, K., Panagiota, T., Shimizu, S., Saito, M., Sejima, T., and Takenaka, A.
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- 2015
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9. 303 Exploration of new predictive factors of post-partial nephrectomy renal function: Is sophisticated film technology or microscopic examination valuable in clinical practice?
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Sejima, T., Yamaguchi, N., Iwamoto, H., Masago, T., Morizane, S., Honda, M., and Takenaka, A.
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- 2015
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10. 176 Unilateral cryptorchidism induces testicular cells' DNA oxidative damage and apoptosis bilaterally in the rat; the effects of antioxidants administration
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Tsounapi, P., Honda, M., Dimitriadis, F., Shimizu, S., Iguchi, M., Imanishi, M., Matsunaga, S., Kawamoto, B., Hikita, K., Muraoka, K., Sejima, T., Sofikitis, N., Saito, M., Tomita, S., and Takenaka, A.
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- 2015
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11. 108 The analysis of predictive factors of renal function after partial nephrectomy: How do host and surgical factors affect the post-operative stable period?
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Sejima, T., Yamaguchi, N., Iwamoto, H., Masago, T., Morizane, S., Honda, M., and Takenaka, A.
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- 2015
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12. 1127 The utility of constitutional implementation of robotic surgery on the basis of a comprehensive department in academic institutional framework
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Sejima, T., Iwamoto, H., Masago, T., Morizane, S., Yao, A., Muraoka, K., Honda, M., Kitano, H., and Takenaka, A.
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- 2014
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13. 297 MicroRNA-210 is up-regulated in the human peripheral blood in the early stage of clear cell renal cell carcinoma
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Iwamoto, H., Osaki, M., Masago, T., Morizane, S., Yao, A., Honda, M., Sejima, T., Okada, F., and Takenaka, A.
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- 2014
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14. 15 Risk stratification model based on robust multi-institutional data stratified by preoperative clinicopathological factors including promising serum biomarkers in patients with upper urinary tract urothelial carcinoma undergoing radical nephroureterectomy
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Morizane, S., Iwamoto, H., Masago, T., Yao, A., Honda, M., Sejima, T., and Takenaka, A.
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- 2014
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15. 951 Renal functional deterioration caused by radical nephrectomy affect comprehensive prognosis in patients with T1 renal cell carcinoma
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Sejima, T., Iwamoto, H., Masago, T., Morizane, S., Yao, A., Isoyama, T., and Takenaka, A.
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- 2013
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16. 925 The prognostic impact of pre-operative hematological disorders and a risk stratification model in bladder cancer patients treated with radical cystectomy
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Sejima, T., Iwamoto, H., Masago, T., Morizane, S., Yao, A., Isoyama, T., Koumi, T., and Takenaka, A.
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- 2013
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17. 339 Histopathological and molecular predictors of chronic kidney disease progression and its related mortality after radical nephrectomy
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Sejima, T., Iwamoto, H., Masago, T., Morizane, S., Yao, A., Isoyama, T., and Takenaka, A.
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- 2013
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18. UP-03.151 Significance of Hemoglobin Value Before Gemcitabine/Cisplatin or Carboplatin Treatment as an Independent Prognostic Biomarker in Advanced Urothelial Cancer
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Morizane, S., Iwamoto, H., Inoue, S., Matsumoto, M., Yao, A., Hinata, N., Honda, M., Isoyama, T., Sejima, T., and Takenaka, A.
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- 2011
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19. UP-02.180 Initial Experience of Iodine-125 (I-125) Permanent Seed-Implant Brachytherapy for Localized Prostate Cancer
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Yao, A., Iwamoto, H., Morizane, S., Hinata, N., Isoyama, T., Michimoto, K., Kodani, K., Sejima, T., Ogawa, T., and Takenaka, A.
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- 2011
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20. Anomalous junction of the pancreaticobiliary duct accompanied by gallbladder cancer and obstructive jaundice in a patient with high serum and bile cytokine levels.
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Akiyama, Takayoshi, Hasegawa, Taisuke, Sejima, Teruhiro, Sahara, Hiroyuki, Kitabayashi, Kazuo, Seto, Keitaro, Saito, Hitoshi, Takashima, Shigeki, Akiyama, T, Hasegawa, T, Sejima, T, Sahara, H, Kitabayashi, K, Seto, K, Saito, H, and Takashima, S
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GALLBLADDER ,JAUNDICE ,CYTOKINES ,BILE duct abnormalities ,ADENOCARCINOMA ,BILE ,BILE ducts ,CHOLESTASIS ,DIFFERENTIAL diagnosis ,ENDOSCOPIC retrograde cholangiopancreatography ,GALLBLADDER tumors ,PANCREATIC duct ,DISEASE complications - Abstract
We report a case of anomalous junction of the pancreaticobiliary duct (AJPBD) associated with gallbladder cancer and obstructive jaundice in a patient with high serum and bile cytokine levels. The patient was a 63-year-old woman who complained of right hypochondralgia. Ultrasound, computed tomography, percutaneous transhepatic cholangiography, and endoscopic retrograde cholangio-pancreatatography revealed dilation of the bile ducts, an elevated lesion of the gallbladder, and AJPBD. She underwent percutaneous transhepatic cholangio-drainage (PTCD) for obstructive jaundice. However, the total bilirubin concentration remained high 7 days after PTCD. Her serum interleukin 6 level was 57,359 pg/ml before PTCD, and gradually decreased to 10 pg/ml after PTCD. Bile interleukin 6 level was 10 pg/ml before PTCD, 8997 pg/ ml 3 h after PTCD and gradually decreased there after. Serum and bile levels of tumor necrosis factor alpha and hepatocyte growth factor were high before and after PTCD. The patient underwent an extended cholecystectomy and resection of the extrahepatic bile duct. The resected specimen showed two elevated lesions of the gallbladder which, microscopically, revealed moderately differentiated tubular adenocarcinoma. These findings suggest that pre-existing inconspicuous inflammation of the biliary tract due to reflux of pancreatic juice is involved in elevation of serum and bile cytokines, and that cytokines may participate in gallbladder carcinogenesis associated with AJPBD. [ABSTRACT FROM AUTHOR]
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- 1998
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21. Preoperative risk stratification models after radical cystectomy for bladder cancer: A multi-center study.
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Yamane H, Morizane S, Honda M, Muraoka K, Oono H, Isoyama T, Ono K, Sejima T, Kadowaki H, and Takenaka A
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Objective: We investigated preoperative patient factors associated with prognosis in 263 bladder cancer (BC) patients undergoing radical cystectomy (RC). We also developed new risk stratification models for prognosis., Methods: This retrospective study included patients treated at Tottori University Hospital and affiliated hospitals between January 2010 and December 2019. The relationship between preoperative patient factors and overall recurrence-free and cancer-specific survival (CSS) was analyzed. The modified Glasgow prognosis score (mGPS) was calculated using serum albumin and C-reactive protein (CRP) levels. Statistical analyses included the log-rank test and Cox proportional hazards regression., Results: Eastern Cooperative Oncology Group performance status (ECOG-PS), mGPS, and clinical tumor stage independently predicted CSS in multivariate analysis. A new risk stratification model included ECOG-PS ≥2, clinical tumor stage ≥3, serum albumin <3.5 g/dL, and serum CRP >0.5 mg/dL. Risk groups were defined as 0 factors (low risk), 1-2 factors (intermediate risk), and 3-4 factors (high risk). High-risk patients showed significantly poorer 3-year cancer-free survival: 86.9% (low risk), 76.7% (intermediate risk), and 50.0% (high risk)., Conclusions: ECOG-PS, clinical tumor stage, and mGPS are predictive of poor cancer-free survival post-RC for BC. Our model offers the potential for prognostic prediction in these patients., (© 2024 The Japanese Urological Association.)
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- 2024
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22. The Benefits of Adjuvant Chemotherapy for Upper Tract Urothelial Carcinoma Require at Least Three Cycles.
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Yumioka T, Morizane S, Muraoka K, Oono H, Isoyama T, Sakaridani N, Ono K, Sejima T, Kadowaki H, Hikita K, Honda M, and Takenaka A
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Background: Upper urinary tract urothelial carcinoma (UTUC) is uncommon. In advanced cases, radical nephroureterectomy (RNU) alone is not curative, and recurrence and metastasis are likely to occur. Adjuvant chemotherapy (AC) is an evidence-based treatment. However, the optimal number of AC cycles is not clear. This multicenter study investigated the number of cycles required for the beneficial effects of AC in Japanese patients with UTUC., Methods: Patients who were diagnosed with UTUC and underwent RNU at our hospital and affiliated hospitals from January 2010 to September 2020 were included in the study. Patients with pathological T3 or higher or lymph node metastasis were observed or given AC, and their responses were compared. The AC regimens included gemcitabine and cisplatin or carboplatin. Patients were also classified into two groups: the observation and two cycles of AC group and the three to four cycles of AC group. The survival curves for recurrence-free survival (RFS) and cancer-specific survival (CSS) were evaluated using Kaplan-Meier analyses., Results: Of the 133 patients enrolled in the study, 24 received 2 cycles of AC, 37 received 3-4 cycles, and 72 were observed only. The 5-year RFS was 67.1% for the 3-4 cycles of AC group and 41.7% for the observation and two cycles of AC group. The 5-year CSS was 72.2% for the 3-4 cycles of AC group and 35.9% for the observation and two cycles of AC group. RFS and CSS were significantly longer in the 3-4 cycles of AC group compared to the observation and 2 cycles group ( P = 0.048 and P = 0.005 respectively)., Conclusion: AC prolonged RFS and CSS in the real-world setting. However, at least three cycles of AC are required to achieve beneficial effects in patients with UTUC., Competing Interests: The authors declare no conflict of interest., (©2024 Tottori University Medical Press.)
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- 2024
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23. The rare urological entity of ureterosciatic hernia: A case report of successful treatment after repeated recurrence.
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Hoshino T, Sejima T, Iwamoto H, and Fukumoto Y
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Ureterosciatic hernia is a rare urological entity. We report a case of successful laparoscopic surgery for a ureterosciatic hernia that recurred repeatedly after conservative treatment. A 73-year-old woman complained of left abdominal pain. Computed tomography (CT) showed a left ureterosciatic hernia. After placement of the ureteral stent, the ureterosciatic hernia improved. The ureteral stent was removed after ureteral migration became normal. Four years and 8 months later, left ureterosciatic hernia recurred. Same treatment was performed; however, it recurred again. Therefore, laparoscopic surgery using a mesh was performed. The patient had no recurrence 9 months postoperatively., Competing Interests: The authors declare no conflicts of interest., (© 2023 The Authors.)
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- 2023
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24. Comprehensive Investigations of Multiple Factors That Are Related to Refractory Outcome in Urosepsis Patients.
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Sejima T, Masago T, Morizane S, Honda M, and Takenaka A
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Background: Urosepsis is an acute life-threating disease, and some cases show refractory outcome to therapy. In an aging society of developed countries, characteristics of urosepsis are becoming complicated. We performed a comprehensive investigation regarding the clinical and social aspects that are related to refractory outcomes in urosepsis patients., Methods: The patient cohort consisted of 66 patients with urosepsis. Multiple factors from clinical and social aspects were reviewed retrospectively. Two categories of refractory outcomes were defined. One was afebrile resistance (AR); fever continued more than 7 days from the initiation of therapy. Another was discharge resistance (DR); hospitalization continued for more than 30 days. Logistic regression analyses were performed to identify significant factors that are related to the AR or DR., Results: Univariate analysis demonstrated that high score of Eastern Cooperative Oncology Group Performance Status (ECOG PS) (≥ 2) and Age-adjusted Charlson comorbidity index (CCI) (≥ 4), high serum C-reactive protein (CRP) level (≥ 14.9 mg/dL), and low serum albumin level (≤ 2.26 g/dL) were significantly related to AR. Univariate analysis results also revealed that high score of ECOG PS (≥ 2), high serum creatinine level (≥ 1.54 mg/dL) and vasopressor administration were significantly related to DR. Multivariate analyses demonstrated that low serum albumin level (≤ 2.26g/dL) was the only significant factor that was related to AR. In contrast, high score of ECOG PS (≥ 2) and high serum creatinine level (≥ 1.54 mg/dL) were significant factors that were related to DR., Conclusion: It is suggested that evaluating serum albumin levels is essential for the therapeutic first step because hypoalbuminemia was the significant factor that was related to obstruction to antipyresis. It is also suggested that the deterioration of patients' activities of daily living and renal dysfunction might be the refractory factors for discharge from the hospital, which was the ultimate therapeutic goal., Competing Interests: The authors declare no conflicts of interest., (©2022 Tottori University Medical Press.)
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- 2022
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25. Disuse muscle atrophy-improving effect of ninjin'yoeito in a mouse model.
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Takemoto R, Sejima T, Han LK, Michihara S, and Takahashi R
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- Adaptor Proteins, Signal Transducing biosynthesis, Adaptor Proteins, Signal Transducing genetics, Animals, Cell Cycle Proteins biosynthesis, Cell Cycle Proteins genetics, Diet, Hindlimb pathology, Hindlimb Suspension, Male, Medicine, Kampo, Mice, Mice, Inbred ICR, Muscle Weakness drug therapy, Muscle, Skeletal pathology, Muscular Atrophy pathology, Muscular Disorders, Atrophic pathology, Organ Size, TOR Serine-Threonine Kinases biosynthesis, TOR Serine-Threonine Kinases genetics, Drugs, Chinese Herbal therapeutic use, Muscular Atrophy drug therapy, Muscular Disorders, Atrophic drug therapy
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Disuse syndrome indicates psychosomatic hypofunction caused by excess rest and motionless and muscle atrophy is termed disuse muscle atrophy. Disuse muscle atrophy-induced muscle weakness and hypoactivity further induces muscle atrophy, leading to a vicious cycle, and this is considered a factor causing secondary sarcopenia and subsequently frailty. Since frailty finally leads to a bedridden state requiring nursing, in facing a super-aging society, intervention for a risk factor of frailty, disuse muscle atrophy, is important. However, the main treatment of disuse muscle atrophy is physical therapy and there are fewer effective preventive and therapeutic drugs. The objective of this study was to search for Kampo medicine with a disuse muscle atrophy-improving effect. Ninjin'yoeito is classified as a qi-blood sohozai (dual supplement) in Chinese herbal medicine, and it has an action supplementing the spleen related to muscle. In addition, improvement of muscle mass and muscle weakness by ninjin'yoeito in a clinical study has been reported. In this study, the effect of ninjin'yoeito on disuse muscle atrophy was investigated. A disuse muscle atrophy model was prepared using male ICR mice. After surgery applying a ring for tail suspension, a 1-week recovery period was set. Ninjin'yoeito was administered by mixing it in the diet for 1 week after the recovery period, followed by tail suspension for 14 days. Ninjin'yoeito administration was continued until autopsy including the hindlimb suspension period. The mice were euthanized and autopsied immediately after completion of tail suspension, and the hindlimb muscles were collected. The food and water intakes during the hindlimb unloaded period, wet weight of the collected muscle, and muscle synthesis and muscle degradation-related factors in blood and muscle were evaluated. Ingestion of ninjin'yoeito inhibited tail suspension-induced reduction of the soleus muscle wet weight. In addition, an increase in the blood level of a muscle synthesis-related factor, IGF-1, and promotion of phosphorylation of mTOR and 4E-BP1 in the soleus muscle were observed. It was suggested that ninjin'yoeito has a disuse muscle atrophy-improving action. Promotion of the muscle synthesis pathway was considered the action mechanism of this., (Copyright © 2021 The Authors. Published by Elsevier Ltd.. All rights reserved.)
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- 2021
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26. Pathological eradication of recurrent metastatic renal cell carcinoma with sarcomatoid component by nivolumab plus ipilimumab combination therapy.
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Sejima T, Masago T, Yoshida M, Nishi T, Kawabata Y, Tajima Y, Yumioka T, Honda M, and Takenaka A
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Although immuno-oncology combination therapy with nivolumab plus ipilimumab has recently shown a notable antitumor effect for first-line metastatic renal cell carcinoma, the eradication of the disease is extremely rare. Moreover, conventional treatments for renal cell carcinoma show little efficacy in the case of a poor prognostic pathological feature of sarcomatoid dedifferentiation. We report a case of pathological eradication of recurrent metastatic renal cell carcinoma with sarcomatoid component after laparoscopic radical nephrectomy by nivolumab plus ipilimumab combination therapy. A 57-year-old male patient received four cycles of nivolumab plus ipilimumab and thirty cycles of nivolumab for local recurrence and liver metastasis after laparoscopic radical nephrectomy for T3a renal cell carcinoma with sarcomatoid component. He underwent partial hepatectomy for the remaining small hepatic lesion after immuno-oncology therapy. Pathology of the surgical specimen showed no viable cancer cells. The patient was alive with no evidence of disease a year after partial hepatectomy. Our case encourages clinicians to achieve a second cure for patients with recurrent metastatic renal cell carcinoma after radical nephrectomy even though the resected kidney pathology showed sarcomatoid component., Competing Interests: Conflict of interestThe authors declare that they have no competing interests., (© The Japan Society of Clinical Oncology 2021.)
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- 2021
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27. Xp11.2 translocation renal cell carcinoma with TFE3 gene fusion in the elderly: case report and literature review.
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Masago T, Kobayakawa S, Ohtani Y, Taniguchi K, Naka T, Kuroda N, Takahashi C, Isoyama T, and Sejima T
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A 68-year-old man was followed up with chronic kidney disease. Follow-up CT incidentally detected a tumor at the left kidney and multiple small nodular shadows in the lungs bilaterally. The patient underwent needle biopsy and was diagnosed with Xp11.2 translocation renal cell carcinoma (RCC) pathologically. Hence, laparoscopic nephrectomy was performed. Fluorescence in situ hybridization analysis revealed a break-apart of the transcription factor E3 (TFE3) genes in the left tumor. After 2 months postoperatively, nivolumab and ipilimumab were administered thrice intravenously, considering the intermediate risk by the IMDC risk classification. However, pleural effusion occurred but was removed adequately. Lung metastasis decreased, but new metastasis occurred at the left iliopsoas muscle. Target therapy was performed with axitinib. Unfortunately, he died 6 months later postoperatively. These tumors commonly occur in children than in adults, and very rare in elderly patients. Xp11.2 translocation RCC in the elderly has a poorer prognosis than that in children. To date, no effective treatment for Xp11.2 translocation RCC has been established., Competing Interests: Conflict of interestThe authors declare that they have no conflict of interest., (© The Japan Society of Clinical Oncology 2020.)
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- 2020
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28. [Mechanism of Inhibitory Effects of Cherry Bark-containing Jumihaidokuto on Benzoyl Peroxide Induced Erythema in Hairless Mice].
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Zhang Q, Michihara S, Sejima T, Han LK, and Takahashi R
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- Acne Vulgaris drug therapy, Animals, Benzoyl Peroxide therapeutic use, Cells, Cultured, Drug Therapy, Combination, Epidermis metabolism, Erythema metabolism, Gene Expression drug effects, Humans, Interleukin-1alpha genetics, Interleukin-1alpha metabolism, Male, Mice, Hairless, Plant Bark chemistry, Plant Extracts isolation & purification, Plant Extracts pharmacology, RNA, Messenger genetics, RNA, Messenger metabolism, Benzoyl Peroxide adverse effects, Erythema chemically induced, Erythema drug therapy, Phytotherapy, Plant Extracts therapeutic use
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Benzoyl peroxide (BPO) has been widely used to treat acne vulgaris. Skin flaking, erythema and skin irritation have been observed as side effects of BPO in the treatment of this disorder. In a clinical study, cherry bark-containing jumihaidokuto significantly reduced the erythema induced by BPO application. However, its mechanism of action has not been clarified. In the present study, an application of 10% BPO caused erythema and an increase in interleukin (IL)-1α in the skin of hairless mice, and these changes were significantly suppressed by cherry bark-containing jumihaidokuto at 600 mg/kg. In addition, using a three-dimensional cultured human epidermis model (LabCyte EPI-MODEL), cherry bark-containing jumihaidokuto extract at 250 or 500 μg/mL significantly suppressed IL-1α mRNA expression induced by the application of 0.2 mM BPO. Therefore, cherry bark-containing jumihaidokuto may have suppressed BPO-induced erythema by inhibiting the increase in the IL-1α level in the skin.
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- 2020
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29. Flutamide as an Alternative Anti-androgen Agent and Predictor of the Efficacy of Novel Androgen Receptor-targeted Agents.
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Yamaguchi N, Morizane S, Yumioka T, Iwamoto H, Hikita K, Sejima T, Honda M, and Takenaka A
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- Abiraterone Acetate therapeutic use, Aged, Aged, 80 and over, Antineoplastic Combined Chemotherapy Protocols therapeutic use, Benzamides, Docetaxel therapeutic use, Humans, Male, Middle Aged, Nitriles, Phenylthiohydantoin analogs & derivatives, Phenylthiohydantoin therapeutic use, Prostate-Specific Antigen blood, Prostatic Neoplasms, Castration-Resistant blood, Receptors, Androgen, Treatment Outcome, Androgen Antagonists therapeutic use, Flutamide therapeutic use, Prostatic Neoplasms, Castration-Resistant drug therapy
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Background/aim: There are few reports that verify the relationship between the therapeutic effects of flutamide and novel androgen receptor-targeted agents. We aimed to evaluate the benefits of flutamide as an alternative anti-androgen agent and its effects on the efficacy of novel androgen receptor-targeted agents., Patients and Methods: Patients with castration-resistant prostate cancer on novel androgen receptor-targeted agents without prior docetaxel therapy were included. Changes in prostate-specific antigen (PSA) level were recorded., Results: Patients who responded well to flutamide (Flutamide effective) following initial maximum androgen blockade (MAB) showed significantly higher changes in serum PSA levels (p=0.039) and PSA-progression-free survival (PFS) rate (p=0.016) following enzalutamide therapy compared to those who did not respond well to flutamide. Multivariate analysis showed that the factor of Flutamide effective was significantly associated with a good PSA-PFS rate following enzalutamide therapy (HR=7.36, 95%CI=1.4-38.71, p=0.018)., Conclusion: Patients showing good response to flutamide following initial MAB may achieve a satisfactory PSA-PFS rate with subsequent enzalutamide therapy., (Copyright© 2019, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.)
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- 2019
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30. Cross-Sectional Investigations of Pre- and Post-Operative Renal Global Function and Renal Parenchymal Volumetry in Both Partial and Radical Nephrectomy Utilizing Film-Based Technology.
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Sejima T, Yumioka T, Yamaguchi N, Masago T, Morizane S, Hikita K, Honda M, and Takenaka A
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Objectives: To investigate pre- and post-operative renal global function and renal parenchymal volume (RPV) in both partial nephrectomy (PN) and radical nephrectomy (RN) utilizing film-based technology., Patients and Methods: The patient cohort consisted of 81 and 82 cases with T1 tumors (≤ 7 cm) treated by PN and RN, respectively. Renal global function was assessed using the estimated glomerular filtration rate (eGFR), and RPV was measured using a Synapse Vincent volumetric analyzer which creates a reconstructed image from a computed tomography scan. Renal global function and RPV were evaluated pre-operatively and 6 months post-operatively., Results: The percent eGFR decline of PN cases was significantly reduced compared with RN cases (p < 0.0001). Post-operative RPV of the contralateral side was significantly increased compared with pre-operative RPV (p < 0.0001) in RN cases. The percent change of the contralat-eral side RPV was slightly increased in RN compared with PN cases (p = 0.1881). A strong positive correlation was noted between post-operative eGFR and post-operative total RPV (r = 0.69, p < 0.0001), and a strong negative correlation was noted between percent eGFR decline and post-operative total RPV (r = -0.63, p < 0.0001)., Conclusions: Prominent compensatory renal hypertrophy occurred within 6 months after RN. Post-operative renal functional outcome was closely correlated with the post-operative total RPV. In addition to RPV, further studies to unravel post-operative renal function from multifactorial aspects including surgical factors and intrinsic medical disease should be performed in the future.
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- 2019
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31. Effective Treatment of Upper Urinary Tract Malignancies Using Laparoscopic Surgery in Three Nonagenarians in Poor General Condition: Are They Too Old for Surgery?
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Sejima T, Morizane S, Hikita K, Honda M, and Takenaka A
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Backgrounds: Surgical procedures in the elderly are associated with higher perioperative morbidity and mortality rates than in younger patients. This is especially significant because elderly individuals are more likely to be operated on now than in the past because they represent the fastest growing subset of the population in advanced countries. Our cases are three nonagenarian patients with renal malignancy in poor general condition and were effectively treated by laparoscopic surgery. Case Presentation: Case 1 was a 91-year-old male patient with a right renal cell carcinoma of pT1b N0 M0. Case 2 was a 92-year-old male patient with a right renal pelvic tumor of pT3 N0 M0. Case 3 was a 90-year-old female patient with a left renal pelvic tumor of pT2 N0 M0. Case 1 had an Eastern Cooperative Oncology Group performance status of 1. The status of cases 2 and 3 was both rated as 2. All three cases had grade 3A chronic kidney disease. Cases 2 and 3 also had deep vein thrombosis in the lower extremities and dementia. In addition, case 2 had coronary occlusive disease. All cases were treated by laparoscopic surgery and effectively discharged from hospital without major physical complications. Conclusion: This report is the first English-language article that describes treating nonagenarian patients by laparoscopic urologic surgery. An increasing number of nonagenarian patients present with urologic malignancies, and surgeons are frequently faced with the question, "Are they too old for surgery?" Our report suggests that laparoscopic surgery for renal malignancy in nonagenarian patients is feasible., Competing Interests: No competing financial interests exist.
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- 2018
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32. Site-dependent differences in the composite fibers of male pelvic plexus branches: an immunohistochemical analysis of donated elderly cadavers.
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Muraoka K, Morizane S, Hieda K, Honda M, Sejima T, Murakami G, Abe SI, and Takenaka A
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- Aged, Aged, 80 and over, Cadaver, Humans, Male, Middle Aged, Nitric Oxide Synthase Type I analysis, Prostate innervation, Rectum innervation, Seminal Vesicles innervation, Urinary Bladder innervation, Vasoactive Intestinal Peptide analysis, Hypogastric Plexus chemistry, Nerve Fibers chemistry, Prostate chemistry, Rectum chemistry, Seminal Vesicles chemistry, Urinary Bladder chemistry
- Abstract
Background: Although the pelvic autonomic plexus branches are considered to be a mixture of sympathetic and parasympathetic nerves, little is known regarding the composite fibers of the pelvic plexus branches. This study aimed to investigate the immunohistochemical features of sympathetic and parasympathetic nerves in the pelvic autonomic plexus branches., Methods: Using 10 donated elderly male cadavers, the detailed topohistology of nerve fibers at and around the bladder, seminal vesicle, prostate, and rectum was examined. Neuronal nitric oxide synthase (nNOS) and vasoactive intestinal polypeptide (VIP) were used as parasympathetic nerve markers; tyrosine hydroxylase (TH) was used as a sympathetic nerve marker. The myenteric plexus of the colon was utilized as a positive control., Results: Most nerve fibers in the bladder, seminal vesicle, prostate, and rectum were both nNOS- and TH-positive. Thus, pelvic plexus branches were classified into two types: 1) triple-positive mixed nerves (nNOS+, VIP+, TH+, thick myelinated fibers + or -) and 2) double-positive mixed nerves (nNOS+, VIP-, TH+, thick myelinated fibers + or -). Notably, triple-positive nerves were localized within the posterosuperior part of the plexus (near the rectum) and travelled anteroinferiorly toward the posterolateral corner of the prostate. The posteriorly and inferiorly located nerves were predominantly composed of parasympathetic, rather than sympathetic, fibers. In contrast, nerve fibers within and along the bladder and seminal vesicle contained either no or few VIP-positive nerves. These superiorly located nerves were characterized by clear sympathetic nerve dominance., Conclusions: The nerves of the pelvic plexus branches were clearly classified into nerves around the bladder and seminal vesicle (VIP-negative) and nerves around the prostate (VIP-positive). Although nNOS- and VIP-positive nerve fibers are candidate cavernous nerves, cavernous nerve identity cannot be definitively concluded for these nerves in the periprostatic region.
- Published
- 2018
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33. The significance of predictable traumatic area by renorrhaphy in the prediction of postoperative ipsilateral renal function.
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Masago T, Yamaguchi N, Iwamoto H, Morizane S, Hikita K, Honda M, Sejima T, and Takenaka A
- Abstract
Introduction: To determine the relationship between the actual renal function loss and volume loss in robot-assisted partial nephrectomy (RAPN) using a novel three-dimensional volume analyzer., Material and Methods: We respectively evaluated the medical records of 23 consecutive patients who underwent RAPN between January 2012 and March 2016 and the data on their kidney function and parenchymal mass specific to the operated kidney. Parenchymal volume was measured by computerized tomography and reconstructed with a Synapse Vincent volumetric analyzer. Using this system, we predicted the renal vascular territory and other trauma areas involved in renorrhaphy. All measurements were taken within 3 and 6 months pre- and postoperatively, respectively., Results: The actual postoperative renal parenchymal volume was significantly correlated with the virtual predicted residual renal volume excluding the tumor and resected margin (r = 0.435, p <0.05). The ratio of split estimated glomerular filtration rate (eGFR) postoperative/preoperative) was significantly correlated with the virtual predicted residual renal volume excluding the resected margin and the traumatic area by renorrhaphy (r = 0.401, p <0.05)., Conclusions: When predicting the reduction of renal function of the diseased side following partial nephrectomy, adding the extent of the area traumatized by renorrhaphy might be useful for predicting the postoperative split renal function of the affected kidney.
- Published
- 2018
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34. Land plants drive photorespiration as higher electron-sink: comparative study of post-illumination transient O 2 -uptake rates from liverworts to angiosperms through ferns and gymnosperms.
- Author
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Hanawa H, Ishizaki K, Nohira K, Takagi D, Shimakawa G, Sejima T, Shaku K, Makino A, and Miyake C
- Subjects
- Carbon Dioxide metabolism, Cell Respiration drug effects, Cell Respiration radiation effects, Cycadopsida drug effects, Cycadopsida radiation effects, Electron Transport drug effects, Electron Transport radiation effects, Ferns drug effects, Ferns radiation effects, Hepatophyta drug effects, Hepatophyta radiation effects, Magnoliopsida drug effects, Magnoliopsida radiation effects, Models, Biological, Oxygen Consumption drug effects, Oxygen Consumption radiation effects, Photosynthesis drug effects, Photosynthesis radiation effects, Photosystem II Protein Complex metabolism, Sodium Bicarbonate pharmacology, Cycadopsida metabolism, Electrons, Ferns metabolism, Hepatophyta metabolism, Light, Magnoliopsida metabolism, Oxygen metabolism
- Abstract
In higher plants, the electron-sink capacity of photorespiration contributes to alleviation of photoinhibition by dissipating excess energy under conditions when photosynthesis is limited. We addressed the question at which point in the evolution of photosynthetic organisms photorespiration began to function as electron sink and replaced the flavodiiron proteins which catalyze the reduction of O
2 at photosystem I in cyanobacteria. Algae do not have a higher activity of photorespiration when CO2 assimilation is limited, and it can therefore not act as an electron sink. Using land plants (liverworts, ferns, gymnosperms, and angiosperms) we compared photorespiration activity and estimated the electron flux driven by photorespiration to evaluate its electron-sink capacity at CO2 -compensation point. In vivo photorespiration activity was estimated by the simultaneous measurement of O2 -exchange rate and chlorophyll fluorescence yield. All C3-plants leaves showed transient O2 -uptake after actinic light illumination (post-illumination transient O2 -uptake), which reflects photorespiration activity. Post-illumination transient O2 -uptake rates increased in the order from liverworts to angiosperms through ferns and gymnosperms. Furthermore, photorespiration-dependent electron flux in photosynthetic linear electron flow was estimated from post-illumination transient O2 -uptake rate and compared with the electron flux in photosynthetic linear electron flow in order to evaluate the electron-sink capacity of photorespiration. The electron-sink capacity at the CO2 -compensation point also increased in the above order. In gymnosperms photorespiration was determined to be the main electron-sink. C3-C4 intermediate species of Flaveria plants showed photorespiration activity, which intermediate between that of C3- and C4-flaveria species. These results indicate that in the first land plants, liverworts, photorespiration started to function as electron sink. According to our hypothesis, the dramatic increase in partial pressure of O2 in the atmosphere about 0.4 billion years ago made it possible to drive photorespiration with higher activity in liverworts., (© 2017 Scandinavian Plant Physiology Society.)- Published
- 2017
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35. A prognostic model for predicting urinary incontinence after robot-assisted radical prostatectomy.
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Honda M, Kawamoto B, Morizane S, Hikita K, Muraoka K, Sejima T, and Takenaka A
- Subjects
- Aged, Humans, Logistic Models, Magnetic Resonance Imaging, Male, Middle Aged, Predictive Value of Tests, Preoperative Period, Prognosis, Proportional Hazards Models, Prostatectomy methods, Prostatic Neoplasms diagnostic imaging, Prostatic Neoplasms physiopathology, Risk Factors, Robotic Surgical Procedures methods, Urethra diagnostic imaging, Urinary Bladder physiopathology, Urinary Incontinence diagnostic imaging, Urinary Incontinence physiopathology, Urinary Tract diagnostic imaging, Urinary Tract physiopathology, Postoperative Complications etiology, Prostatectomy adverse effects, Prostatic Neoplasms surgery, Robotic Surgical Procedures adverse effects, Urinary Incontinence etiology
- Abstract
Background: The aim of this study is to develop a novel prognostic model for estimating the risk of postoperative urinary incontinence (UI) after robot-assisted radical prostatectomy (RARP)., Methods: Participants comprised 131 men who underwent RARP at our hospital from 2011 to 2013. Cox regression analyses were performed to evaluate associations between UI and preoperative factors including filling cystometry and pressure-flow study results and magnetic resonance imaging., Results: Logistic regression analyses revealed significant associations between membranous urethral length or levator thickness and UI at 3 and 6 months. Stratification produced high (membranous urethral length < 9.5 mm or levator thickness < 9.0 mm) and low (membranous urethral length ≥ 9.5 mm and levator thickness ≥ 9.0 mm) UI risk groups. These inter-group differences in UI rate were significant., Conclusions: We developed a novel prognostic model based on preoperative patient data that can be used for patient counselling., (Copyright © 2016 John Wiley & Sons, Ltd.)
- Published
- 2017
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36. Comprehensive Analysis of Factors Affecting Post-partial Nephrectomy Renal Global Function.
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Sejima T, Yumioka T, Yamaguchi N, Iwamoto H, Masago T, Morizane S, Honda M, and Takenaka A
- Abstract
Background: To explore new factors that are predictive of post-partial nephrectomy (PN) renal global function, we analyzed various clinico-pathological factors with a special focus on renal volume measured via three-dimensional imaging technology and histopathological parameters in non-neoplastic parenchyma., Methods: Estimated glomerular filtration rate (eGFR) and computed tomography (CT) scan were examined pre- and 6 months. post-operatively in 52 patients treated by PN. The post-operative percent eGFR decline was employed as the measure of global renal functional deterioration. The novel factors analyzed included the percent renal parenchymal volume decline of the diseased side, contralateral and bilateral sides and the global glomerulosclerosis (GS) extent in non-neoplastic parenchyma. Renal parenchymal volumetry by CT scan was performed using SYNAPSE VINCENT (Fujifilm). Additional factors analyzed included patient demographics and comorbidities, surgical factors and tumor pathology. All factors demonstrating significant tendencies ( P < 0.1) in univariate analyses were subjected to multivariate logistic regression analysis., Results: Two groups were categorized according to the degree of eGFR decline. Groups A and B were categorized as less than 15% and greater than 15% decline, respectively. Pre-operative eGFR was significantly lower in group B than in group A. Greater than 10% global GS extent in non-neoplastic parenchyma, male gender and proteinuria were significantly more frequent in in group B than in group A. The renal volume change was not statistically significant. In multivariate logistic regression analysis, greater than 10% global GS extent in non-neoplastic parenchyma was the sole independent affecting factor for Group B., Conclusion: Our study suggested that host factors rather than surgical factors may be useful for the prediction of post-PN renal global function. The evaluation of the global GS extent in non-neoplastic parenchyma is a promising biomarker of post-PN renal global function.
- Published
- 2017
37. Identification of MicroRNAs Involved in Resistance to Sunitinib in Renal Cell Carcinoma Cells.
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Yamaguchi N, Osaki M, Onuma K, Yumioka T, Iwamoto H, Sejima T, Kugoh H, Takenaka A, and Okada F
- Subjects
- Cell Line, Tumor, Humans, Sunitinib, Antineoplastic Agents pharmacology, Carcinoma, Renal Cell genetics, Drug Resistance, Neoplasm genetics, Indoles pharmacology, Kidney Neoplasms genetics, MicroRNAs genetics, Pyrroles pharmacology
- Abstract
Aim: To generate sunitinib-resistant renal cell carcinoma (RCC) cell lines and identify miRNAs contributing to sunitinib resistance., Materials and Methods: Two RCC cell lines, ACHN and RCC23, were cultured by continuous treatment with sunitinib for 3 months, with doses gradually increasing up to the 50% inhibitory concentration for each cell line. We performed microarray and quantitative real-time polymerase chain reaction analyses of sunitinib-resistant ACHN (SR-ACHN) and RCC23 (SR-RCC23) cells, as well of as sunitinib-sensitive ACHN and RCC23 cells., Results: SR-ACHN and SR-RCC23 cells exhibited significantly higher resistance to sunitinib treatment compared to sunitinib-sensitive cells. SR-ACHN and SR-RCC23 cells were hypertrophic and contained granules in the cytoplasm. When SR-ACHN and SR-RCC23 cells were compared to ACHN and RCC23 cells, expression of miR-575, miR-642b-3p, and miR-4430 was significantly increased, while that of miR-18a-5p, miR-29b-1-5p, miR-431-3p, and miR-4521 was significantly decreased., Conclusion: These miRNAs may contribute to sunitinib resistance in humans., (Copyright© 2017, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.)
- Published
- 2017
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38. Evaluation of Incontinence after Robot-Assisted Laparoscopic Radical Prostatectomy: Using the International Consultation on Incontinence Modular Questionnaire Short Form and Noting the Number of Safety Pads Needed by Japanese Patients.
- Author
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Hikita K, Honda M, Kawamoto B, Tsounapi P, Muraoka K, Sejima T, and Takenaka A
- Abstract
Background: The definition of continence after robot-assisted laparoscopic radical prostatectomy (RARP) has not been consistently defined, with many studies only noting the amount of safety pads used. We therefore examined what definition of continence would be appropriate, employing both the International Consultation on Incontinence Modular Questionnaire Short Form (ICIQ-SF) and the number of pads used by patients. We also evaluated the relationship between the number of pads used and degree of incontinence., Methods: Patients who underwent RARP between October 2010 and July 2014 in our department were included in the present study. All patients were evaluated by ICIQ-SF and the number of pads used 1, 3, 6 and 12 months after surgery. Frequency of incontinence and amount of incontinence were evaluated by ICIQ-SF Questions 1 and 2 respectively at 12 months. Quality of life (QOL) was evaluated by ICIQ-SF Question 3 at 1, 3, 6, and 12 months after RARP., Results: The overall study population was 156 patients. In Question 1, 19 patients answered that they leaked urine several times a day, but 5 patients did not use pads, and 8 patients were using only 1 pad a day. In Question 2, 8 patients answered that they leaked a moderate amount of urine, but 2 patients did not use any pads, and 3 patients used only 1 pad a day. QOL showed significant differences between using no pad, 1 pad, or ≥ 2 pads at 1, 3, 6, and 12 months after RARP., Conclusion: Simply noting the number of pads used by a patient is an insufficient way to evaluate incontinence after RARP. Assessment by an appropriate questionnaire is also needed.
- Published
- 2017
39. The Liverwort, Marchantia , Drives Alternative Electron Flow Using a Flavodiiron Protein to Protect PSI.
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Shimakawa G, Ishizaki K, Tsukamoto S, Tanaka M, Sejima T, and Miyake C
- Subjects
- Chlorophyll metabolism, Cytochrome b6f Complex genetics, Cytochrome b6f Complex metabolism, Electron Transport genetics, Flavoproteins genetics, Gene Expression Regulation, Plant, Light, Marchantia genetics, Mutation, Oxygen metabolism, Photosynthesis genetics, Photosynthesis radiation effects, Photosystem I Protein Complex genetics, Photosystem II Protein Complex genetics, Photosystem II Protein Complex metabolism, Plant Proteins genetics, Proton-Motive Force radiation effects, Reverse Transcriptase Polymerase Chain Reaction, Time Factors, Flavoproteins metabolism, Marchantia metabolism, Photosystem I Protein Complex metabolism, Plant Proteins metabolism
- Abstract
The diffusion efficiency of oxygen in the atmosphere, like that of CO
2 , is approximately 104 times greater than that in aqueous environments. Consequently, terrestrial photosynthetic organisms need mechanisms to protect against potential oxidative damage. The liverwort Marchantia polymorpha , a basal land plant, has habitats where it is exposed to both water and the atmosphere. Furthermore, like cyanobacteria, M. polymorpha has genes encoding flavodiiron proteins (FLV). In cyanobacteria, FLVs mediate oxygen-dependent alternative electron flow (AEF) to suppress the production of reactive oxygen species. Here, we investigated whether FLVs are required for the protection of photosynthesis in M. polymorpha A mutant deficient in the FLV1 isozyme (Δ MpFlv1 ) sustained photooxidative damage to photosystem I (PSI) following repetitive short-saturation pulses of light. Compared with the wild type (Takaragaike-1), Δ MpFlv1 showed the same photosynthetic oxygen evolution rate but a lower electron transport rate during the induction phase of photosynthesis. Additionally, the reaction center chlorophyll in PSI, P700, was highly reduced in Δ MpFlv1 but not in Takaragaike-1. These results indicate that the gene product of MpFlv1 drives AEF to oxidize PSI, as in cyanobacteria. Furthermore, FLV-mediated AEF supports the production of a proton motive force to possibly induce the nonphotochemical quenching of chlorophyll fluorescence and suppress electron transport in the cytochrome b6 / f complex. After submerging the thalli, a decrease in photosystem II operating efficiency was observed, particularly in Δ MpFlv1 , which implies that species living in these sorts of habitats require FLV-mediated AEF., (© 2017 American Society of Plant Biologists. All Rights Reserved.)- Published
- 2017
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40. Impact of postoperative phosphodiesterase type 5 inhibitor treatment on lower urinary tract symptoms after robot-assisted radical prostatectomy: a longitudinal study.
- Author
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Honda M, Kawamoto B, Morizane S, Hikita K, Muraoka K, Sejima T, and Takenaka A
- Subjects
- Aged, Humans, Longitudinal Studies, Lower Urinary Tract Symptoms physiopathology, Male, Middle Aged, Retrospective Studies, Robotic Surgical Procedures rehabilitation, Treatment Outcome, Urinary Bladder, Overactive physiopathology, Urinary Incontinence physiopathology, Lower Urinary Tract Symptoms drug therapy, Phosphodiesterase 5 Inhibitors therapeutic use, Postoperative Care, Prostatectomy rehabilitation, Prostatic Neoplasms surgery, Tadalafil therapeutic use
- Abstract
Objective: This study aimed to assess the role of tadalafil, a phosphodiesterase type 5 inhibitor (PDE5-I), in recovery of lower urinary tract symptoms (LUTS) after robot-assisted radical prostatectomy (RARP)., Materials and Methods: All patients treated with RARP between October 2010 and August 2013 were enrolled in this retrospective study on prospectively collected data. Patients were retrospectively divided into groups according to postoperative treatment: patients taking tadalafil twice weekly from 1 month to 6 months after RARP, and patients not taking tadalafil. The International Prostate Symptom Score (IPSS), the Overactive Bladder Symptom Score (OABSS) and urinary continence (UC) were assessed preoperatively (2 days before RARP) and at 1, 3, 6, 9 and 12 months after RARP., Results: There was no difference in the overall IPSS score between the groups. At 6 and 9 months, the IPSS storage symptom scores were significantly lower in patients taking tadalafil than in patients not taking tadalafil. In patients with overactive bladder (OAB) before RARP (n = 22), no differences in the total scores of OABSS were seen between patients taking tadalafil and patients not taking tadalafil. On the other hand, in non-OAB patients before RARP (n = 68), at 3, 6 and 9 months, the total scores of OABSS were significantly lower in patients taking tadalafil than in patients not taking tadalafil. No differences in UC rates after surgery were seen between the groups., Conclusion: Postoperative tadalafil treatment may be temporarily effective for the recovery of the IPSS storage symptom scores and OABSS.
- Published
- 2017
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41. Biochemical Recurrence Prediction in High-Risk Prostate Cancer Patients, Following Robot-Assisted Radical Prostatectomy.
- Author
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Yamaguchi N, Yumioka T, Iwamoto H, Masago T, Morizane S, Honda M, Sejima T, and Takenaka A
- Abstract
Background: High-risk prostate cancer treatment has been controversial. Some high-risk prostate cancer patients fail to respond to radical prostatectomy only. Thus, we aimed to investigate the predictive factors for biochemical recurrence (BCR) and identify patients who could achieve sufficient therapeutic effect by radical prostatectomy only., Methods: Of 264 medical records reviewed, 141 low-intermediate-risk and 100 high-risk prostate cancer patients, excluding those who had received neoadjuvant hormone therapy, were analyzed. BCR was defined as the first increase in prostate-specific antigen levels (≥ 0.2 ng/mL), with levels not decreasing to undetectable limits, after radical prostatectomy. Log-rank test and Cox proportional hazards regression analyses were performed to determine the prognostic factors. We investigated the perioperative predictive factors for BCR and BCR-free survival rates, with the number of National Comprehensive Cancer Network (NCCN) high-risk factors for high-risk prostate cancer patients who underwent robot-assisted radical prostatectomy., Results: Multivariate analyses showed that clinical T3 was significantly associated with BCR [hazard ratio (HR) = 4.052; 95% confidence interval (CI), 1.26-12.99; P = 0.019]. Of the 100 patients, 77 had 1 high-risk factor and 23 had ≥ 2 high-risk factors; the 1-year BCR-free survival rate of patients with 1 high-risk factor and those with ≥ 2 high-risk factors was 94.8% and 69.6%, respectively. Patients with ≥ 2 high-risk factors were significantly associated with BCR ( P = 0.002). No difference in BCR rate between patients with 1 high-risk factor and those with low- and intermediate-risk was found., Conclusion: High-risk prostate cancer patients with 1 NCCN high-risk factor can be considered for robot-assisted radical prostatectomy treatment only.
- Published
- 2016
42. Photorespiration provides the chance of cyclic electron flow to operate for the redox-regulation of P700 in photosynthetic electron transport system of sunflower leaves.
- Author
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Takagi D, Hashiguchi M, Sejima T, Makino A, and Miyake C
- Subjects
- Carbon Dioxide metabolism, Chlorophyll metabolism, Chloroplasts metabolism, Electron Transport, Electrons, Helianthus radiation effects, Light, Oxidation-Reduction, Oxygen metabolism, Plant Leaves physiology, Plant Leaves radiation effects, Plastoquinone metabolism, Stress, Physiological, Helianthus physiology, Photosynthesis physiology, Photosystem I Protein Complex physiology, Photosystem II Protein Complex physiology
- Abstract
To elucidate the molecular mechanism to oxidize the reaction center chlorophyll, P700, in PSI, we researched the effects of partial pressure of O2 (pO2) on photosynthetic characteristic parameters in sunflower (Helianthus annuus L.) leaves. Under low CO2 conditions, the oxidation of P700 was stimulated; however the decrease in pO2 suppressed its oxidation. Electron fluxes in PSII [Y(II)] and PSI [Y(I)] showed pO2-dependence at low CO2 conditions. H(+)-consumption rate, estimated from Y(II) and CO2-fixation/photorespiration rates (JgH(+)), showed the positive curvature relationship with the dissipation rate of electrochromic shift signal (V H (+) ), which indicates H(+)-efflux rate from lumen to stroma in chloroplasts. Therefore, these electron fluxes contained, besides CO2-fixation/photorespiration-dependent electron fluxes, non-H(+)-consumption electron fluxes including Mehler-ascorbate peroxidase (MAP)-pathway. Y(I) that was larger than Y(II) surely implies the functioning of cyclic electron flow (CEF). Both MAP-pathway and CEF were suppressed at lower pO2, with plastoquinone-pool reduced. That is, photorespiration prepares the redox-poise of photosynthetic electron transport system for CEF activity as an electron sink. Excess Y(II), [ΔY(II)] giving the curvature relationship with V H (+) , and excess Y(I) [ΔCEF] giving the difference between Y(I) and Y(II) were used as an indicator of MAP-pathway and CEF activity, respectively. Although ΔY(II) was negligible and did not show positive relationship to the oxidation-state of P700, ΔCEF showed positive linear relationship to the oxidation-state of P700. These facts indicate that CEF cooperatively with photorespiration regulates the redox-state of P700 to suppress the over-reduction in PSI under environmental stress conditions.
- Published
- 2016
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43. Robot-assisted radical cystectomy is a promising alternative to open surgery in the Japanese population with a high rate of octogenarians.
- Author
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Iwamoto H, Yumioka T, Yamaguchi N, Masago T, Morizane S, Honda M, Sejima T, and Takenaka A
- Subjects
- Adult, Aged, Aged, 80 and over, Blood Loss, Surgical statistics & numerical data, Blood Transfusion statistics & numerical data, Female, Humans, Japan, Lymph Nodes pathology, Male, Middle Aged, Urinary Bladder Neoplasms pathology, Asian People statistics & numerical data, Cystectomy adverse effects, Cystectomy methods, Robotic Surgical Procedures adverse effects, Urinary Bladder Neoplasms surgery
- Abstract
Background: Japanese patients who undergo radical cystectomy have a unique feature, i.e., the ratio of octogenarians is higher than in Western patients because of the aging Japanese population. The purpose of this study was to compare perioperative outcomes, complications, and oncologic outcomes between robot-assisted radical cystectomy and open radical cystectomy., Methods: From 2010-2015, 20 (of which 8 were octogenarians) and 40 (of which 11 were octogenarians) patients underwent robot-assisted radical cystectomy and open radical cystectomy, respectively., Results: There was no significant difference in terms of patient demographics between the two surgical cohorts, regardless of patient age. In the octogenarian patient cohort, the rates of previous abdominal surgery and the median age-adjusted Charlson comorbidity index were higher. The median estimated blood loss, transfusion rate and the days to resumption of a regular diet were significantly lower in the robot-assisted radical cystectomy cohort, regardless of patient age. Robot-assisted radical cystectomy significantly decreased grade II or higher complications, but there were no significant differences in terms of decreasing grade III or higher complications, regardless of patient age. Positive surgical margin and the mean number of lymph nodes removed were similar between the two surgical cohorts, regardless of patient age., Conclusion: Although robot-assisted radical cystectomy has a short history in Japan, the outcomes were generally comparable to those of previous studies. In a country that is aging rapidly like Japan, robot-assisted radical cystectomy could be a valid option for the management of muscle-invasive and high-risk superficial bladder cancer.
- Published
- 2016
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44. Superoxide and Singlet Oxygen Produced within the Thylakoid Membranes Both Cause Photosystem I Photoinhibition.
- Author
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Takagi D, Takumi S, Hashiguchi M, Sejima T, and Miyake C
- Subjects
- Ascorbate Peroxidases metabolism, Chlorophyll metabolism, Chloroplasts drug effects, Chloroplasts metabolism, Ionophores pharmacology, Nigericin pharmacology, Oxygen pharmacology, Paraquat pharmacology, Photosystem I Protein Complex physiology, Plant Proteins metabolism, Reactive Oxygen Species metabolism, Spinacia oleracea drug effects, Superoxide Dismutase metabolism, Photosystem I Protein Complex metabolism, Singlet Oxygen metabolism, Spinacia oleracea physiology, Superoxides metabolism, Thylakoids metabolism
- Abstract
Photosystem I (PSI) photoinhibition suppresses plant photosynthesis and growth. However, the mechanism underlying PSI photoinhibition has not been fully clarified. In this study, in order to investigate the mechanism of PSI photoinhibition in higher plants, we applied repetitive short-pulse (rSP) illumination, which causes PSI-specific photoinhibition in chloroplasts isolated from spinach leaves. We found that rSP treatment caused PSI photoinhibition, but not PSII photoinhibition in isolated chloroplasts in the presence of O2 However, chloroplastic superoxide dismutase and ascorbate peroxidase activities failed to protect PSI from its photoinhibition. Importantly, PSI photoinhibition was largely alleviated in the presence of methyl viologen, which stimulates the production of reactive oxygen species (ROS) at the stromal region by accepting electrons from PSI, even under the conditions where CuZn-superoxide dismutase and ascorbate peroxidase activities were inactivated by KCN. These results suggest that the ROS production site, but not the ROS production rate, is critical for PSI photoinhibition. Furthermore, we found that not only superoxide (O2 (-)) but also singlet oxygen ((1)O2) is involved in PSI photoinhibition induced by rSP treatment. From these results, we suggest that PSI photoinhibition is caused by both O2 (-) and (1)O2 produced within the thylakoid membranes when electron carriers in PSI become highly reduced. Here, we show, to our knowledge, new insight into the PSI photoinhibition in higher plants., (© 2016 American Society of Plant Biologists. All Rights Reserved.)
- Published
- 2016
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45. Transurethral En Bloc Resection of Bladder Tumor Using an Endoscopic Submucosal Dissection Technique: Preliminary Results in an Animal Model.
- Author
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Morizane S, Sejima T, Iwamoto H, Masago T, Honda M, Ikebuchi Y, Matsumoto K, Ueki M, and Takenaka A
- Abstract
Background: Transurethral resection of bladder tumor (TURBT) technique has been considered the routine method for removing most bladder tumors for decades. In contrast, endoscopic submucosal dissection (ESD) is the gold-standard treatment for gastrointestinal superficial tumors. We investigated the effectiveness and applicability of a new technique for en bloc bladder tumor resection using ESD procedure., Methods: Four Landrace Large White Duroc female pigs were anesthetized with isoflurane prior endoscopic resection using a large-caliber prototype fiber bronchoscope. After local infiltration of the submucosa with sodium hyaluronate using an injector needle, a section of the target area (1.0-2.0 cm diameter circular area) was cut with the Dual Knife., Results: In total, seven target sections were resected from the pigs. The median size of the resected sections was 1.8 cm (range 1.0-2.5 cm) and the median time taken to perform the resection of one section was 20 min (range 4-35 min). These target sections were completely resected en bloc. Although the small bladder perforations occurred on two occasions, no other short-term complications such as uncontrollable bleeding were observed., Conclusion: This procedure is a slightly difficult in the pigs with thin bladder walls. However, this procedure with the slim flexible cystoscope may allow us to be able to remove bladder tumors using only light sedation, especially for cases when small tumor recurrence is observed during routine cystoscopy for the patients with non-muscle invasive bladder cancer.
- Published
- 2016
46. The First Pilot Comprehensive Evaluation of the Outcomes of Different Types of Robotic Surgeries in the Different Surgical Departments: The Penta, Tetra and Trifecta Achievements in Robotic Surgeries.
- Author
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Sejima T, Morizane S, Fujiwara K, Ashida K, Saito H, Taniguchi Y, Nakamura H, and Takenaka A
- Abstract
Background: To ensure safe performance in robotic surgery, the Minimal Invasive Surgery Center (MISC) is composed of the anesthesiology department, five surgery departments and co-medical staff in our institution. The objective of this study was to evaluate the outcomes of different types of robotic surgeries for cancer treatment (n = 326) from different surgery departments in the MISC., Methods: The outcomes of robot-assisted radical prostatectomy (RARP), partial nephrectomy (RAPN), transoral robotic surgery (TORS) for pharyngeal cancer, and robotic surgery for lung, gastric and rectal cancer were evaluated using the similar concept of pentafecta in RARP., Results: The pentafecta rates of RARP and robotic surgery for rectal cancer were 33.3 and 56.5%, respectively. The tetrafecta rates of RARP (excluding potency evaluation from pentafecta) and TORS were 48.4 and 57.1%, respectively. The trifecta rates of RAPN, robotic surgeries for lung and gastric cancer were 75.9, 74.2 and 84.2%, respectively. The failure of tetrafecta in RARP achievement was significantly associated with high risk than with low risk according to National Comprehensive Cancer Network classification., Conclusion: This is the world's first comprehensive evaluation of different types of robotic surgeries for cancer treatment in the constitutional framework of an academic institution. MISC, which provides the constitutional framework of an academic institution, is providing immeasurable benefits in terms of robotic surgery quality, and it may ultimately lead to high penta-, tetra-, and trifecta rates for robotic surgeries for cancer treatment in all surgical departments.
- Published
- 2016
47. Characterization of mild and severe post-radical nephrectomy renal functional deterioration utilizing histopathological evaluation of non-neoplastic nephrectomized renal parenchyma.
- Author
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Sejima T, Yumioka T, Yamaguchi N, Iwamoto H, Masago T, Morizane S, Honda M, and Takenaka A
- Subjects
- Adult, Aged, Aged, 80 and over, Arteriosclerosis complications, Arteriosclerosis pathology, Female, Glomerular Filtration Rate, Glomerulonephritis complications, Humans, Kidney Neoplasms pathology, Male, Middle Aged, Postoperative Period, Prognosis, Renal Insufficiency etiology, Survival Rate, Carcinoma, Renal Cell surgery, Cardiovascular Diseases mortality, Glomerulonephritis pathology, Kidney Glomerulus pathology, Kidney Neoplasms surgery, Nephrectomy adverse effects, Renal Insufficiency physiopathology
- Abstract
Background: We investigated factors affecting mild (MRFD) and severe renal functional deterioration (SRFD) after radical nephrectomy with a special focus on the histopathology of nephrectomized non-neoplastic renal parenchyma., Methods: MRFD was defined as a postoperative decline of percent estimated glomerular filtration rate (eGFR) lower than the value of the mean minus standard deviation (SD). SRFD was defined as a rate greater than the value of the mean plus SD. The histopathological factors of global glomerulosclerosis (GS) and arteriosclerosis in non-neoplastic renal parenchyma and multiple clinical factors were analyzed to determine whether they affected postoperative renal functional deterioration in 100 renal cell carcinoma cases. The prognoses, including non-cancer mortality, were collected from long-term follow-up data., Results: A higher preoperative eGFR and a higher global GS extent in non-neoplastic renal parenchyma were independently associated with MRFD and SRFD, respectively. The cardiovascular disease-specific survival rates of the SRFD group and the group with global GS extent >14 % were significantly worse than those of their counterparts., Conclusions: This is the first report to identify global GS extent in nephrectomized non-neoplastic renal parenchyma as a factor affecting the development of life-threatening post-nephrectomy renal functional deterioration. Moreover, we are the first to advocate the importance of the characterization of favorable post-nephrectomy renal functional deterioration. The identification of MRFD and SRFD by histopathological evaluation of nephrectomized non-neoplastic renal parenchyma will contribute to personalized postoperative follow-up. It may improve follow-up of individual patients with SRFD by permitting collaboration with other clinical departments such as cardiology.
- Published
- 2016
- Full Text
- View/download PDF
48. [Lymph node dissection].
- Author
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Sejima T and Takenaka A
- Subjects
- Humans, Lymph Node Excision adverse effects, Lymphatic Metastasis, Male, Meta-Analysis as Topic, Postoperative Complications, Lymph Node Excision methods, Prostatic Neoplasms surgery
- Published
- 2016
49. Spinal glycine transporter-1 inhibition influences the micturition reflex in urethane-anesthetized rats.
- Author
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Honda M, Hikita K, Kawamoto B, Muraoka K, Shimizu S, Saito M, Sejima T, Chancellor MB, Yoshimura N, and Takenaka A
- Subjects
- Anesthetics, Intravenous pharmacology, Animals, Disease Models, Animal, Female, Glycine Plasma Membrane Transport Proteins metabolism, Injections, Spinal, Rats, Rats, Sprague-Dawley, Reflex, Sarcosine administration & dosage, Urinary Bladder drug effects, Urinary Bladder metabolism, Urinary Bladder, Overactive metabolism, Urinary Bladder, Overactive physiopathology, Glycine Plasma Membrane Transport Proteins antagonists & inhibitors, Sarcosine analogs & derivatives, Spinal Cord metabolism, Urethane pharmacology, Urinary Bladder physiopathology, Urinary Bladder, Overactive drug therapy, Urination drug effects
- Abstract
Purpose: Glycine is an inhibitory neurotransmitter in the central nervous system. So far, two types of glycine transporters (GlyTs), GlyT-1 and GlyT-2, have been cloned. The aim of this study is to investigate the effects of a selective GlyT-1 inhibitor that can increase endogenous glycine concentration on the micturition reflex in urethane-anesthetized rats., Methods: Continuous cystometrograms (0.04 ml/min) were performed in female Sprague-Dawley rats (232-265 g) under urethane anesthesia. After stable micturition cycles were established, ALX5407, a selective GlyT-1 inhibitor, was administered intrathecally or intracerebroventricularly to evaluate changes in bladder activity. Cystometric parameters were recorded and compared before and after drug administration., Results: Intrathecal administration of ALX5407 (1, 3, 10 and 30 μg) increased intercontraction intervals at doses of 3 μg or higher in a dose-dependent fashion. Intrathecal administration of ALX5407 (1, 3, 10 and 30 μg) also increased pressure threshold at doses of 3 μg or higher in a dose-dependent fashion. However, when ALX5407 (1, 3, 10 and 30 μg) was administered intracerebroventricularly, there were no significant changes in intercontraction intervals, pressure threshold, maximum voiding pressure or baseline pressure or post-void residual urine volume at any doses tested., Conclusion: The results of our study indicate that GlyT-1 plays an important role in the modulation of micturition. Furthermore, these findings indicate that in urethane-anesthetized rats suppression of GlyT-1 can inhibit the micturition reflex at the spinal cord level. Thus, GlyT-1 could be a potential target for the treatment of bladder dysfunction such as overactive bladder.
- Published
- 2016
- Full Text
- View/download PDF
50. Comparison of the overactive bladder symptom score and the overactive bladder symptom score derived from the bladder diaries.
- Author
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Hikita KS, Honda M, Hirano S, Kawamoto B, Panagiota T, Muraoka K, Sejima T, and Takenaka A
- Subjects
- Adult, Aged, Aged, 80 and over, Female, Humans, Male, Middle Aged, Nocturnal Enuresis physiopathology, Predictive Value of Tests, Reproducibility of Results, Retrospective Studies, Severity of Illness Index, Time Factors, Urinary Bladder, Overactive physiopathology, Urinary Incontinence, Urge physiopathology, Urodynamics, Nocturnal Enuresis diagnosis, Surveys and Questionnaires, Urinary Bladder physiopathology, Urinary Bladder, Overactive diagnosis, Urinary Incontinence, Urge diagnosis
- Abstract
Aims: The overactive bladder symptom score (OABSS) is a useful tool for assessing the four key symptoms of overactive bladder (OAB), but it sometimes misrepresents a patient's actual voiding status. To examine whether the patient-determined OABSS underestimates or overestimates the true status, its results were compared to those of the OABSS derived from a 7-day bladder diary (OABSS-BD)., Methods: Records of patients who visited our outpatient clinic with lower urinary tract symptoms were evaluated retrospectively. The patients were asked to complete the OABSS and the 7-day bladder diary (BD). The OABSS-BD was created from the 7-day BD. Questions were compared between the OABSS and the OABSS-BD., Results: A total of 44 men and 31 women were evaluated. For daytime frequency, the mean OABSS score was 1.03 ± 0.57 and the OABSS-BD score was 0.69 ± 0.52 (P < 0.01). For nighttime frequency, the mean OABSS score was 2.27 ± 0.84, and the OABSS-BD score was 1.96 ± 1.00 (P = 0.04). For urinary urgency, the mean OABSS score was 2.49 ± 1.83, and the OABSS-BD score was 2.70 ± 1.90 (P = 0.27). For urgency incontinence, the mean OABSS score was 1.67 ± 1.92, and the OABSS-BD score was 1.52 ± 1.87 (P = 0.28). For the total score, the mean OABSS total score was 7.26 ± 3.92, and the OABSS-BD score was 6.98 ± 3.26 (P = 0.23)., Conclusions: The OABSS is a very simple and useful tool. However, compared to the results from the 7-day FVC, the present patients overestimated daytime and nighttime frequency., (© 2015 Wiley Periodicals, Inc.)
- Published
- 2016
- Full Text
- View/download PDF
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