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431 results on '"Segall, Jeffrey E"'

1. Mechanical compression regulates tumor spheroid invasion into a 3D collagen matrix

Author

Pandey, Mrinal, Suh, Young Joon, Kim, Minha, Davis, Hannah Jane, Segall, Jeffrey E, and Wu, Mingming

Subjects
Quantitative Biology - Cell Behavior
Abstract

Uncontrolled growth of tumor cells in confined spaces leads to the accumulation of compressive stress within the tumor. Although the effects of tension within 3D extracellular matrices on tumor growth and invasion are well established, the role of compression in tumor mechanics and invasion is largely unexplored. In this study, we modified a Transwell assay such that it provides constant compressive loads to spheroids embedded within a collagen matrix. We used microscopic imaging to follow the single cell dynamics of the cells within the spheroids, as well as invasion into the 3D extracellular matrices (EMCs). Our experimental results showed that malignant breast tumor (MDA-MB-231) and non-tumorigenic epithelial (MCF10A) spheroids responded differently to a constant compression. Cells within the malignant spheroids became more motile within the spheroids and invaded more into the ECM under compression; whereas cells within non-tumorigenic MCF10A spheroids became less motile within the spheroids and did not display apparent detachment from the spheroids under compression. These findings suggest that compression may play differential roles in healthy and pathogenic epithelial tissues and highlights the importance of tumor mechanics and invasion., Comment: 10 pages, 5 figure and 3 supplementary figures. Phys. Biol. 2024

Published
2023
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2. Tumor Spheroid Chemotaxis in Epidermal Growth Factor Gradients Revealed by a 3D Microfluidic Device

Author

Suh, Young Joon, Pandey, Mrinal, Segall, Jeffrey E, and Wu, Mingming

Subjects
Quantitative Biology - Cell Behavior
Abstract

Epidermal growth factor (EGF), a potent cytokine, is known to promote tumor invasion both in vivo and in vitro. Previously, we observed that single breast tumor cells (MDA-MB-231 cell line) embedded within a 3D collagen matrix displayed enhanced motility but no discernible chemotaxis in the presence of linear EGF gradients using a microfluidic platform. Inspired by a recent theoretical development that clustered mammalian cells respond differently to chemical gradients than single cells, we studied tumor spheroid invasion within a 3D extracellular matrix (ECM) in the presence of EGF gradients. We found that EGF gradients promoted tumor cell detachment from the spheroid core, and the position of the tumor spheroid core showed a mild chemotactic response towards the EGF gradients. For those tumor cells detached from the spheroids, they showed an enhanced chemokinesis response in contrast to previous experimental results using single cells embedded within an ECM. No discernible chemotactic response towards the EGF gradients was found for the cells outside the spheroid core. This work demonstrates that a cluster of tumor cells responds differently than single tumor cells towards EGF gradients and highlights the importance of a tumor spheroid platform for chemotaxis studies., Comment: 12 pages, 4 figures, and 3 supplementary figures

Published
2021
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3. Spatial and temporal dynamics of RhoA activities of single breast tumor cells in a 3D environment revealed by a machine learning-assisted FRET technique

Author

Cheung, Brian CH, Hodgson, Louis, Segall, Jeffrey E, and Wu, Mingming

Subjects
Quantitative Biology - Cell Behavior, Quantitative Biology - Quantitative Methods, Quantitative Biology - Subcellular Processes
Abstract

One of the hallmarks of cancer cells is their exceptional ability to migrate within the extracellular matrix (ECM) for gaining access to the circulatory system, a critical step of cancer metastasis. RhoA, a small GTPase, is known to be a key molecular switch that toggles between actomyosin contractility and lamellipodial protrusion during cell migration. Current understanding of RhoA activity in cell migration has been largely derived from studies of cells plated on a two-dimensional (2D) substrate using a FRET biosensor. There has been increasing evidence that cells behave differently in a more physiologically relevant three-dimensional (3D) environment, however, studies of RhoA activities in 3D have been hindered by low signal-to-noise ratio in fluorescence imaging. In this paper, we present a machine learning-assisted FRET technique to follow the spatiotemporal dynamics of RhoA activities of single breast tumor cells (MDA-MB-231) migrating in a 3D as well as a 2D environment using a RhoA biosensor. We found that RhoA activity is more polarized along the long axis of the cell for single cells migrating on 2D fibronectin-coated glass versus those embedded in 3D collagen matrices. In particular, RhoA activities of cells in 2D exhibit a distinct front-to-back and back-to-front movement during migration in contrast to those in 3D. Finally, regardless of dimensionality, RhoA polarization is found to be correlated with cell shape., Comment: 10 pages, 5 figures, 7 supplementary figures

Published
2021
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4. The architecture of co-culture spheroids regulates tumor invasion within a 3D extracellular matrix

Author

Huang, Yu Ling, Shiau, Carina, Wu, Cindy, Segall, Jeffrey E., and Wu, Mingming

Subjects
Quantitative Biology - Cell Behavior
Abstract

Tumor invasion, the process by which tumor cells break away from their primary tumor and gain access to vascular systems, is an important step in cancer metastasis. Most current 3D tumor invasion assays consisted of single tumor cells embedded within an extracellular matrix (ECM). These assays taught us much of what we know today on how key biophysical (e.g. ECM stiffness) and biochemical (e.g. cytokine gradients) parameters within the tumor microenvironment guided and regulated tumor invasion. One limitation of the single tumor cell invasion assay was that it did not account for cell-cell adhesion within the tumor. In this article, we developed a micrometer scale 3D co-culture spheroid invasion assay that was compatible with microscopic imaging. Micrometer scale co-culture spheroids (1:1 ratio of metastatic breast cancer MDA-MB-231 and non-tumorigenic epithelial MCF-10A cells) were made using an array of microwells, and then were embedded within a collagen matrix in a microfluidic platform. Real time imaging of tumor spheroid invasion revealed that the spatial distribution of the two cell types within the tumor spheroid critically regulated tumor invasion. This work linked tumor architecture with tumor invasion and highlighted the importance of the biophysical cues within the bulk of the tumor in tumor invasion., Comment: 15 pages, 5 figures, and 2 pages of supplementary information

Published
2020

5. Supplementary Tables 1 from Functional Contribution and Clinical Implication of Cancer-Associated Fibroblasts in Glioblastoma

Author

Galbo, Phillip M., primary, Madsen, Anne Tranberg, primary, Liu, Yang, primary, Peng, Mou, primary, Wei, Yao, primary, Ciesielski, Michael J., primary, Fenstermaker, Robert A., primary, Graff, Sarah, primary, Montagna, Cristina, primary, Segall, Jeffrey E., primary, Sidoli, Simone, primary, Zang, Xingxing, primary, and Zheng, Deyou, primary

Published
2024
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6. Data from Functional Contribution and Clinical Implication of Cancer-Associated Fibroblasts in Glioblastoma

Author

Galbo, Phillip M., primary, Madsen, Anne Tranberg, primary, Liu, Yang, primary, Peng, Mou, primary, Wei, Yao, primary, Ciesielski, Michael J., primary, Fenstermaker, Robert A., primary, Graff, Sarah, primary, Montagna, Cristina, primary, Segall, Jeffrey E., primary, Sidoli, Simone, primary, Zang, Xingxing, primary, and Zheng, Deyou, primary

Published
2024
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7. Supplementary Methods, Results, Figures 1 from Functional Contribution and Clinical Implication of Cancer-Associated Fibroblasts in Glioblastoma

Author

Galbo, Phillip M., primary, Madsen, Anne Tranberg, primary, Liu, Yang, primary, Peng, Mou, primary, Wei, Yao, primary, Ciesielski, Michael J., primary, Fenstermaker, Robert A., primary, Graff, Sarah, primary, Montagna, Cristina, primary, Segall, Jeffrey E., primary, Sidoli, Simone, primary, Zang, Xingxing, primary, and Zheng, Deyou, primary

Published
2024
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8. Functional Contribution and Clinical Implication of Cancer-Associated Fibroblasts in Glioblastoma

Author

Galbo, Phillip M., primary, Madsen, Anne Tranberg., additional, Liu, Yang, additional, Peng, Mou, additional, Wei, Yao, additional, Ciesielski, Michael J., additional, Fenstermaker, Robert A., additional, Graff, Sarah, additional, Montagna, Cristina, additional, Segall, Jeffrey E., additional, Sidoli, Simone, additional, Zang, Xingxing, additional, and Zheng, Deyou, additional

Published
2023
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11. Live tumor imaging shows macrophage induction and TMEM-mediated enrichment of cancer stem cells during metastatic dissemination

Author

Sharma, Ved P., Tang, Binwu, Wang, Yarong, Duran, Camille L., Karagiannis, George S., Xue, Emily A., Entenberg, David, Borriello, Lucia, Coste, Anouchka, Eddy, Robert J., Kim, Gina, Ye, Xianjun, Jones, Joan G., Grunblatt, Eli, Agi, Nathan, Roy, Sweta, Bandyopadhyaya, Gargi, Adler, Esther, Surve, Chinmay R., Esposito, Dominic, Goswami, Sumanta, Segall, Jeffrey E., Guo, Wenjun, Condeelis, John S., Wakefield, Lalage M., and Oktay, Maja H.

Published
2021
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14. Post-EMT: Cadherin-11 mediates cancer hijacking fibroblasts

Author

Kang, Weirong, primary, Fan, Yibo, additional, Du, Yinxiao, additional, Tonkova, Elina A., additional, Hsu, Yi-Hsin, additional, Tan, Kel Vin, additional, Alexander, Stephanie, additional, Wong, Bin Sheng, additional, Yang, Haocheng, additional, Luo, Jingyuan, additional, Yao, Kuo, additional, Yang, Jiayao, additional, Hu, Xin, additional, Liu, Tingting, additional, Gan, Yu, additional, Zhang, Jian, additional, Zhao, Jean J., additional, Konstantopoulos, Konstantinos, additional, Friedl, Peter, additional, Khong, Pek Lan, additional, Lu, Aiping, additional, Hung, Mien-Chie, additional, Brenner, Michael B., additional, Segall, Jeffrey E., additional, and Gu, Zhizhan, additional

Published
2023
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22. Supplementary Video: HPV- (HN31) from Palbociclib Renders Human Papilloma Virus–Negative Head and Neck Squamous Cell Carcinoma Vulnerable to the Senolytic Agent Navitoclax

Author

Gadsden, Nicholas J., primary, Fulcher, Cory D., primary, Li, Daniel, primary, Shrivastava, Nitisha, primary, Thomas, Carlos, primary, Segall, Jeffrey E., primary, Prystowsky, Michael B., primary, Schlecht, Nicolas F., primary, Gavathiotis, Evripidis, primary, and Ow, Thomas J., primary

Published
2023
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23. Supplementary Video: HPV+ (UMSCC47) from Palbociclib Renders Human Papilloma Virus–Negative Head and Neck Squamous Cell Carcinoma Vulnerable to the Senolytic Agent Navitoclax

Author

Gadsden, Nicholas J., primary, Fulcher, Cory D., primary, Li, Daniel, primary, Shrivastava, Nitisha, primary, Thomas, Carlos, primary, Segall, Jeffrey E., primary, Prystowsky, Michael B., primary, Schlecht, Nicolas F., primary, Gavathiotis, Evripidis, primary, and Ow, Thomas J., primary

Published
2023
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24. Supplementary Figure 1 & 2 from Palbociclib Renders Human Papilloma Virus–Negative Head and Neck Squamous Cell Carcinoma Vulnerable to the Senolytic Agent Navitoclax

Author

Gadsden, Nicholas J., primary, Fulcher, Cory D., primary, Li, Daniel, primary, Shrivastava, Nitisha, primary, Thomas, Carlos, primary, Segall, Jeffrey E., primary, Prystowsky, Michael B., primary, Schlecht, Nicolas F., primary, Gavathiotis, Evripidis, primary, and Ow, Thomas J., primary

Published
2023
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34. Supplementary Figure Legends 1-2 from Differential Enhancement of Breast Cancer Cell Motility and Metastasis by Helical and Kinase Domain Mutations of Class IA Phosphoinositide 3-Kinase

Author

Pang, Huan, primary, Flinn, Rory, primary, Patsialou, Antonia, primary, Wyckoff, Jeffrey, primary, Roussos, Evanthia T., primary, Wu, Haiyan, primary, Pozzuto, Maria, primary, Goswami, Sumanta, primary, Condeelis, John S., primary, Bresnick, Anne R., primary, Segall, Jeffrey E., primary, and Backer, Jonathan M., primary

Published
2023
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35. Supplementary Figure 1 from Differential Enhancement of Breast Cancer Cell Motility and Metastasis by Helical and Kinase Domain Mutations of Class IA Phosphoinositide 3-Kinase

Author

Pang, Huan, primary, Flinn, Rory, primary, Patsialou, Antonia, primary, Wyckoff, Jeffrey, primary, Roussos, Evanthia T., primary, Wu, Haiyan, primary, Pozzuto, Maria, primary, Goswami, Sumanta, primary, Condeelis, John S., primary, Bresnick, Anne R., primary, Segall, Jeffrey E., primary, and Backer, Jonathan M., primary

Published
2023
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42. Supplementary Figure 2 from Differential Enhancement of Breast Cancer Cell Motility and Metastasis by Helical and Kinase Domain Mutations of Class IA Phosphoinositide 3-Kinase

Author

Pang, Huan, primary, Flinn, Rory, primary, Patsialou, Antonia, primary, Wyckoff, Jeffrey, primary, Roussos, Evanthia T., primary, Wu, Haiyan, primary, Pozzuto, Maria, primary, Goswami, Sumanta, primary, Condeelis, John S., primary, Bresnick, Anne R., primary, Segall, Jeffrey E., primary, and Backer, Jonathan M., primary

Published
2023
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48. Supplementary Movie 1 from Differential Enhancement of Breast Cancer Cell Motility and Metastasis by Helical and Kinase Domain Mutations of Class IA Phosphoinositide 3-Kinase

Author

Pang, Huan, primary, Flinn, Rory, primary, Patsialou, Antonia, primary, Wyckoff, Jeffrey, primary, Roussos, Evanthia T., primary, Wu, Haiyan, primary, Pozzuto, Maria, primary, Goswami, Sumanta, primary, Condeelis, John S., primary, Bresnick, Anne R., primary, Segall, Jeffrey E., primary, and Backer, Jonathan M., primary

Published
2023
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