1. RT-PCR Assays for Seven Serotypes of Epizootic Haemorrhagic Disease Virus & Their Use to Type Strains from the Mediterranean Region and North America
- Author
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Kyriaki Nomikou, David E. Stallknecht, Donna J. Johnson, Hafsa Madani, Hagai Yadin, Simon J. Anthony, Narender S. Maan, Carrie Batten, Andrew B. Allison, Mehdi El Harrak, Serife Incoglu, Kadir Yeşilbağ, Peter P. C. Mertens, Sushila Maan, Uludağ Üniversitesi/Veterinerlik Fakültesi/Klinik Öncesi Bilimler Bölümü., Yeşilbağ, Kadir, and ABE-7662-2020
- Subjects
Serotype ,Science & technology - other topics ,Southern Europe ,Public Health and Epidemiology/Infectious Diseases ,lcsh:Medicine ,Polymerase chain-reaction ,Neutralization ,Serology ,law.invention ,0403 veterinary science ,White-tailed deer ,Virus isolation ,law ,Cricetinae ,Oligonucleotide ,Bos ,Molecular genetics ,Mediterranean region ,lcsh:Science ,Evaluation ,Reverse transcriptase polymerase chain reaction ,Phylogeny ,Polymerase chain reaction ,0303 health sciences ,Genome ,Multidisciplinary ,biology ,medicine.diagnostic_test ,Protein VP2 ,Virus typing ,Seg 2 gene ,Virus gene ,Virology/Diagnosis ,04 agricultural and veterinary sciences ,Virus neutralization ,Classification ,Virus strain ,3. Good health ,Sequence-analysis ,Real-time polymerase chain reaction ,Differentiation ,Mammalia ,Reverse transcription polymerase chain reaction ,Antibody ,Epizootic Hemorrhagic Disease Virus ,African Horse Sickness Virus ,Competitive elisa ,Bluetongue-virus ,Research Article ,040301 veterinary sciences ,Capsid protein ,Cattle Diseases ,Protein targeting ,Virology/Emerging Viral Diseases ,Antibodies ,Multidisciplinary sciences ,Article ,Mammal ,Virus ,03 medical and health sciences ,Molecular sequence data ,Virology ,Infectious Diseases/Viral Infections ,Genetics ,Hamster ,medicine ,Animals ,Reoviridae infections ,Orbivirus ,Virus genome ,030304 developmental biology ,Hemorrhagic disease virus, epizootic ,Animal disease ,Reovirus infection ,lcsh:R ,Methodology ,Proteins ,Neutralizing antibody ,Nonhuman ,North america ,Ibaraki-virus ,Capsid proteins ,Isolation and purification ,Epizootic hemorrhagic disease virus ,Immunoassay ,biology.protein ,Cattle disease ,lcsh:Q ,Cattle ,Miridae ,Cell line ,Controlled study ,Nucleotide sequence - Abstract
Epizootic haemorrhagic disease virus (EHDV) infects wild ruminants, causing a frequently fatal haemorrhagic disease. However, it can also cause bluetongue-like disease in cattle, involving significant levels of morbidity and mortality, highlighting a need for more rapid and reliable diagnostic assays. EHDV outer-capsid protein VP2 (encoded by genomesegment 2 [Seg-2]) is highly variable and represents the primary target for neutralising antibodies generated by the mammalian host. Consequently VP2 is also the primary determinant of virus "serotype", as identified in virus neutralisation tests (VNT). Although previous reports have indicated eight to ten EHDV serotypes, recent serological comparisons and molecular analyses of Seg-2 indicate only seven EHDV "types". Oligonucleotide primers were developed targeting Seg-2, for use in conventional RT-PCR assays to detect and identify these seven types. These assays, which are more rapid and sensitive, still show complete agreement with VNT and were used to identify recent EHDV isolates from the Mediterranean region and North America. UK Research & Innovation (UKRI) Biotechnology and Biological Sciences Research Council (BBSRC) CGIAR European Commission (SANCO/940/2002) Department for Environment, Food & Rural Affairs (DEFRA) (SE2617) European Commission European Commission Joint Research Centre UK Research & Innovation (UKRI) Biotechnology and Biological Sciences Research Council (BBSRC) (BBS/E/I/00001159)
- Published
- 2010
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