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2. Laboratory-Based Correlative Soft X-ray and Fluorescence Microscopy in an Integrated Setup

Author

Reinhard, Julius, Kaleta, Sophia, Abel, Johann Jakob, Wiesner, Felix, Wünsche, Martin, Seemann, Eric, Westermann, Martin, Weber, Thomas, Nathanael, Jan, Iliou, Alexander, Fiedorowicz, Henryk, Hillmann, Falk, Eggeling, Christian, Paulus, Gerhard G., and Fuchs, Silvio

Subjects
Physics - Instrumentation and Detectors, Physics - Optics
Abstract

Correlative microscopy is a powerful technique that combines the advantages of multiple imaging modalities to achieve a comprehensive understanding of investigated samples. For example, fluorescence microscopy provides unique functional contrast by imaging only specifically labeled components, especially in biological samples. However, the achievable structural information on the sample in its full complexity is limited. Here, the intrinsic label-free carbon contrast of water window soft X-ray microscopy can complement fluorescence images in a correlative approach ultimately combining nanoscale structural resolution with functional contrast. However, soft X-ray microscopes are complex and elaborate, and typically require a large-scale synchrotron radiation source due to the demanding photon flux requirements. Yet, with modern high-power lasers it has become possible to generate sufficient photon flux from laser-produced plasmas, thus enabling laboratory-based setups. Here, we present a compact table-top soft X-ray microscope with an integrated epifluorescence modality for 'in-situ' correlative imaging. Samples remain in place when switching between modalities, ensuring identical measurement conditions and avoiding sample alteration or destruction. We demonstrate our new method by multimodal images of several exemplary samples ranging from nanoparticles to various multicolor labeled cell types. A structural resolution of down to 50 nm was reached., Comment: 15 pages, 8 figures, preprint

Published
2023
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4. Caveolin-1 dolines form a distinct and rapid caveolae-independent mechanoadaptation system

Author

Lolo, Fidel-Nicolás, Walani, Nikhil, Seemann, Eric, Zalvidea, Dobryna, Pavón, Dácil María, Cojoc, Gheorghe, Zamai, Moreno, Viaris de Lesegno, Christine, Martínez de Benito, Fernando, Sánchez-Álvarez, Miguel, Uriarte, Juan José, Echarri, Asier, Jiménez-Carretero, Daniel, Escolano, Joan-Carles, Sánchez, Susana A., Caiolfa, Valeria R., Navajas, Daniel, Trepat, Xavier, Guck, Jochen, Lamaze, Christophe, Roca-Cusachs, Pere, Kessels, Michael M., Qualmann, Britta, Arroyo, Marino, and del Pozo, Miguel A.

Published
2023
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6. Evaluating the Impact of Supplemental Instruction on Short- and Long-Term Retention of Course Content

Author

Price, Jodi, Lumpkin, Amber G., and Seemann, Eric A.

Abstract

Findings from prior research in various content domains have indicated that Peer Assisted Study Sessions (PASS) attendees earned higher final grades than non-attendees. However, what makes PASS effective remains unknown; for example, PASS could improve short-term retention but hinder long-term maintenance of course content given that some methods that facilitate short-term retention of information hurt long-term retention. Of additional interest was what predicts students' attendance in PASS. We tracked 75 introductory psychology students' short- and long-term retention of course content via unit quizzes and a cumulative final exam, respectively, to determine if PASS attendance improved both types of retention. Results indicated that PASS attendees had significantly higher academic self-efficacy and final grades than non-attendees. Attendees outperformed non-attendees on three of the six quizzes and on the cumulative final exam, supporting both the short- and long-term effectiveness of PASS. (Contains 1 figure, 3 tables and 5 footnotes.)

Published
2012

7. Laboratory-Based Correlative Soft X-ray and Fluorescence Microscopy in an Integrated Setup

Author

Reinhard, Julius, primary, Kaleta, Sophia, additional, Abel, Johann Jakob, additional, Wiesner, Felix, additional, Wünsche, Martin, additional, Seemann, Eric, additional, Westermann, Martin, additional, Weber, Thomas, additional, Nathanael, Jan, additional, Iliou, Alexander, additional, Fiedorowicz, Henryk, additional, Hillmann, Falk, additional, Eggeling, Christian, additional, Paulus, Gerhard G, additional, and Fuchs, Silvio, additional

Published
2023
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9. Then and Now: Technology and the Changing Face of Higher Education.

Author

Seemann, Eric A., Buboltz, Walter C., and Wilkinson, Lamar

Abstract

This project examines the impact of technology on the process of higher education and the impact of these advances on both students and faculty. Student demographics and academic achievement from 30 years ago are compared to those of modern students as reported by veteran and new faculty members. The impact of technology on the modern student is discussed, as are technology-relevant changes in the university experience for students and faculty. The changes in the accessibility of college classes by nontraditional students due to multimedia and Internet technology is also discussed with respect to the changing nature of distance education, including emergence of teleconference courses, Internet-based classes, and other alternatives to the traditional correspondence course. (Author/MSE)

Published
2000

14. Correlative soft X-ray and fluorescence microscopy in the water window region in an integrated laboratory-based setup

Author

Kaleta, Sophia, primary, Reinhard, Julius, additional, Wiesner, Felix, additional, Abel, Johann Jakob, additional, Wünsche, Martin, additional, Westermann, Martin, additional, Seemann, Eric, additional, Reglinski, Katharina, additional, Franke, Christian, additional, Iliou, Alexander, additional, Hillmann, Falk, additional, Eggeling, Christian, additional, Fuchs, Silvio, additional, and Paulus, Gerhard G., additional

Published
2023
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15. Heteromeric clusters of ubiquitinated ER-shaping proteins drive ER-phagy

Author

Foronda, Hector, primary, Fu, Yangxue, additional, Covarrubias-Pinto, Adriana, additional, Bocker, Hartmut T., additional, González, Alexis, additional, Seemann, Eric, additional, Franzka, Patricia, additional, Bock, Andrea, additional, Bhaskara, Ramachandra M., additional, Liebmann, Lutz, additional, Hoffmann, Marina E., additional, Katona, Istvan, additional, Koch, Nicole, additional, Weis, Joachim, additional, Kurth, Ingo, additional, Gleeson, Joseph G., additional, Reggiori, Fulvio, additional, Hummer, Gerhard, additional, Kessels, Michael M., additional, Qualmann, Britta, additional, Mari, Muriel, additional, Dikić, Ivan, additional, and Hübner, Christian A., additional

Published
2023
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16. Ubiquitination regulates ER-phagy and remodelling of endoplasmic reticulum

Author

González, Alexis, primary, Covarrubias-Pinto, Adriana, additional, Bhaskara, Ramachandra M., additional, Glogger, Marius, additional, Kuncha, Santosh K., additional, Xavier, Audrey, additional, Seemann, Eric, additional, Misra, Mohit, additional, Hoffmann, Marina E., additional, Bräuning, Bastian, additional, Balakrishnan, Ashwin, additional, Qualmann, Britta, additional, Dötsch, Volker, additional, Schulman, Brenda A., additional, Kessels, Michael M., additional, Hübner, Christian A., additional, Heilemann, Mike, additional, Hummer, Gerhard, additional, and Dikić, Ivan, additional

Published
2023
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17. Caveolin-1 dolines form a distinct and rapid caveolae-independent mechanoadaptation system

Author

Lolo, Fidel-Nicolás, primary, Walani, Nikhil, additional, Seemann, Eric, additional, Zalvidea, Dobryna, additional, Pavón, Dácil María, additional, Cojoc, Gheorghe, additional, Zamai, Moreno, additional, Viaris de Lesegno, Christine, additional, Martínez de Benito, Fernando, additional, Sánchez-Álvarez, Miguel, additional, Uriarte, Juan José, additional, Echarri, Asier, additional, Jiménez-Carretero, Daniel, additional, Escolano, Joan-Carles, additional, Sánchez, Susana A., additional, Caiolfa, Valeria R., additional, Navajas, Daniel, additional, Trepat, Xavier, additional, Guck, Jochen, additional, Lamaze, Christophe, additional, Roca-Cusachs, Pere, additional, Kessels, Michael M., additional, Qualmann, Britta, additional, Arroyo, Marino, additional, and del Pozo, Miguel A., additional

Published
2022
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20. Caveolin-1 dolines form a distinct and rapid caveolae-independent mechanoadaptation system

Author

Universitat Politècnica de Catalunya. Departament d'Enginyeria Civil i Ambiental, Universitat Politècnica de Catalunya. LACÀN - Mètodes Numèrics en Ciències Aplicades i Enginyeria, Lolo, Fidel Nicolás, Walani, Nikhil, Seemann, Eric, Zalvidea, Dobryna, Pavón, Dácil María, Cojoc, Gheorghe, Zamai, Moreno, Viaris de Lesegno, Christine, Martínez de Benito, Fernando, Sánchez Álvarez, Miguel, Uriarte, Juan José, Echarri, Asier, Jiménez Carretero, Daniel, Escolano, Joan Carles, Sánchez Donoso, Susana Angélica, Arroyo Balaguer, Marino, Universitat Politècnica de Catalunya. Departament d'Enginyeria Civil i Ambiental, Universitat Politècnica de Catalunya. LACÀN - Mètodes Numèrics en Ciències Aplicades i Enginyeria, Lolo, Fidel Nicolás, Walani, Nikhil, Seemann, Eric, Zalvidea, Dobryna, Pavón, Dácil María, Cojoc, Gheorghe, Zamai, Moreno, Viaris de Lesegno, Christine, Martínez de Benito, Fernando, Sánchez Álvarez, Miguel, Uriarte, Juan José, Echarri, Asier, Jiménez Carretero, Daniel, Escolano, Joan Carles, Sánchez Donoso, Susana Angélica, and Arroyo Balaguer, Marino

Abstract

In response to diferent types and intensities of mechanical force, cells modulate their physical properties and adapt their plasma membrane (PM). Caveolae are PM nano-invaginations that contribute to mechanoadaptation, bufering tension changes. However, whether core caveolar proteins contribute to PM tension accommodation independently from the caveolar assembly is unknown. Here we provide experimental and computational evidence supporting that caveolin-1 confers deformability and mechanoprotection independently from caveolae, through modulation of PM curvature. Freeze-fracture electron microscopy reveals that caveolin-1 stabilizes non-caveolar invaginations—dolines—capable of responding to low-medium mechanical forces, impacting downstream mechanotransduction and conferring mechanoprotection to cells devoid of caveolae. Upon cavin-1/PTRF binding, doline size is restricted and membrane bufering is limited to relatively high forces, capable of fattening caveolae. Thus, caveolae and dolines constitute two distinct albeit complementary components of a bufering system that allows cells to adapt efciently to a broad range of mechanical stimuli., Peer Reviewed, Postprint (published version)

Published
2022

22. Ego Identity Status: A Step in the Differentiation Process.

Author

Johnson, Patrick, Buboltz, Walter C., and Seemann, Eric

Abstract

Examines the relationship between identity development and differentiation levels in young adults. A total of 259 participants completed the Extended Version of the Objective Measure of Ego Identity Status, the Differentiation of Self Inventory, and demographic questions. Results showed that each identity status had a unique effect on the various components of differentiation. (Contains 23 references.) (GCP)

Published
2003

25. Candida pathogens induce protective mitochondria-associated type I interferon signalling and a damage-driven response in vaginal epithelial cells

Author

Barcelona Supercomputing Center, Pekmezovic, Marina, Hovhannisyan, Hrant, Gresnigt, Mark S., Iracane, Elise, Oliveira Pacheco, João, Siscar Lewin, Sofía, Seemann, Eric, Qualmann, Britta, Kalkreuter, Till, Müller, Sylvia, Kamradt, Thomas, Mogavero, Selene, Brunke, Sascha, Butler, Geraldine, Gabaldón, Toni, Hube, Bernhard, Barcelona Supercomputing Center, Pekmezovic, Marina, Hovhannisyan, Hrant, Gresnigt, Mark S., Iracane, Elise, Oliveira Pacheco, João, Siscar Lewin, Sofía, Seemann, Eric, Qualmann, Britta, Kalkreuter, Till, Müller, Sylvia, Kamradt, Thomas, Mogavero, Selene, Brunke, Sascha, Butler, Geraldine, Gabaldón, Toni, and Hube, Bernhard

Abstract

Vaginal candidiasis is an extremely common disease predominantly caused by four phylogenetically diverse species: Candida albicans; Candida glabrata; Candida parapsilosis; and Candida tropicalis. Using a time course infection model of vaginal epithelial cells and dual RNA sequencing, we show that these species exhibit distinct pathogenicity patterns, which are defined by highly species-specific transcriptional profiles during infection of vaginal epithelial cells. In contrast, host cells exhibit a homogeneous response to all species at the early stages of infection, which is characterized by sublethal mitochondrial signalling inducing a protective type I interferon response. At the later stages, the transcriptional response of the host diverges in a species-dependent manner. This divergence is primarily driven by the extent of epithelial damage elicited by species-specific mechanisms, such as secretion of the toxin candidalysin by C. albicans. Our results uncover a dynamic, biphasic response of vaginal epithelial cells to Candida species, which is characterized by protective mitochondria-associated type I interferon signalling and a species-specific damage-driven response., M.P., H.H., E.I., J.O.P., T.G., G.B. and B.H. received funding from the European Union Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant no. 642095 (OPATHY). B.H. also received support from the German Research Foundation within the Collaborative Research Centre/Transregio 124 FungiNet (project C1). M.S.G. was supported by the German Research Foundation Emmy Noether Programme (project no. 434385622/GR 5617/1-1). We acknowledge the support of the Spanish Ministry of Science, Innovation and Universities (grant no. PGC2018-099921-B-I00) to the European Molecular Biology Laboratory partnership, the Centro de Excelencia Severo Ochoa and the CERCA Programme/Generalitat de Catalunya. We thank C. Kämnitz from the Electron Microscopy Center in Jena for the sample preparation for TEM. The schematic models in Figs. 4–6 were created with images adapted from Servier Medical Art (Servier)., Peer Reviewed, Postprint (author's final draft)

Published
2021

28. Adolescents' health habits, coping styles and self-concept are predicted by exposure to interparental conflict

Author

Michael, Kerry C., Torres, Aurora, and Seemann, Eric A.

Subjects
Interpersonal conflict -- Influence, Interpersonal conflict -- Health aspects, Domestic relations -- Research, Teenagers -- Health aspects, Teenagers -- Social aspects, Youth -- Health aspects, Youth -- Social aspects, Family and marriage
Abstract

We compared self-reported health habits, coping styles (CRI), and self-concept (MSCS) in late adolescents (17-19 year-olds) with high- and low-conflict family history. The data confirmed that high family conflict had a negative impact on a variety of health habits or related attitudes, including less sleep, and compromised well-being. Total self-concept scores were lower for high-conflict students as well as scores in the Family, Affective, and Competence subscales. High family conflict also fostered coping styles that were avoidant. The data lend support for bio-psychosocial models that postulate family conflict produces stress which can, in turn, have a negative impact on health, doi: 10.1300/J087v48n01_09 [Article copies available for a fee from The Haworth Document Delivery Service: 1-800-HAWORTH. E-mail address: Website: [c] 2007 by The Haworth Press, Inc. All rights reserved.] KEYWORDS. Interparental conflict, adolescents, health, stress, coping, self-concept

Published
2007

29. The Na+/H+ Exchanger Nhe1 Modulates Network Excitability via GABA Release

Author

Bocker, Hartmut T., Heinrich, Theresa, Liebmann, Lutz, Hennings, J. Christopher, Seemann, Eric, Gerth, Melanie, Jakovcevski, Igor, Preobraschenski, Julia, Kessels, Michael M., Westermann, Martin, Isbrandt, Dirk, Jahn, Reinhard, Qualmann, Britta, Huebner, Christian A., Bocker, Hartmut T., Heinrich, Theresa, Liebmann, Lutz, Hennings, J. Christopher, Seemann, Eric, Gerth, Melanie, Jakovcevski, Igor, Preobraschenski, Julia, Kessels, Michael M., Westermann, Martin, Isbrandt, Dirk, Jahn, Reinhard, Qualmann, Britta, and Huebner, Christian A.

Abstract

Brain functions are extremely sensitive to pH changes because of the pH-dependence of proteins involved in neuronal excitability and synaptic transmission. Here, we show that the Na+/H+ exchanger Nhe1, which uses the Na+ gradient to extrude H+, is expressed at both inhibitory and excitatory presynapses. We disrupted Nhe1 specifically in mice either in Emx1- positive glutamatergic neurons or in parvalbumin-positive cells, mainly GABAergic interneurons. While Nhe1 disruption in excitatory neurons had no effect on overall network excitability, mice with disruption of Nhe1 in parvalbumin-positive neurons displayed epileptic activity. From our electrophysiological analyses in the CA1 of the hippocampus, we conclude that the disruption in parvalbumin-positive neurons impairs the release of GABA-loaded vesicles, but increases the size of GABA quanta. The latter is most likely an indirect pH-dependent effect, as Nhe1 was not expressed in purified synaptic vesicles itself. Conclusively, our data provide first evidence that Nhe1 affects network excitability via modulation of inhibitory interneurons.

Published
2019

32. The Na+/H+ Exchanger Nhe1 Modulates Network Excitability via GABA Release

Author

Bocker, Hartmut T, primary, Heinrich, Theresa, additional, Liebmann, Lutz, additional, Hennings, J Christopher, additional, Seemann, Eric, additional, Gerth, Melanie, additional, Jakovčevski, Igor, additional, Preobraschenski, Julia, additional, Kessels, Michael M, additional, Westermann, Martin, additional, Isbrandt, Dirk, additional, Jahn, Reinhard, additional, Qualmann, Britta, additional, and Hübner, Christian A, additional

Published
2018
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35. Author response: Deciphering caveolar functions by syndapin III KO-mediated impairment of caveolar invagination

Author

Seemann, Eric, primary, Sun, Minxuan, additional, Krueger, Sarah, additional, Tröger, Jessica, additional, Hou, Wenya, additional, Haag, Natja, additional, Schüler, Susann, additional, Westermann, Martin, additional, Huebner, Christian A, additional, Romeike, Bernd, additional, Kessels, Michael M, additional, and Qualmann, Britta, additional

Published
2017
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42. Lineup Identification: Combating the Effect of Post-Identification Feedback.

Author

Neuschatz, Jeffrey S., Neuschatz, Joseph S., Seemann, Eric A., and Noble, Anne P.

Subjects
EYEWITNESS identification, PSYCHOLOGICAL feedback, RECOLLECTION (Psychology), CONFIDENCE, SUSPICION, LEGAL evidence
Abstract

The article talks about the eyewitness' struggle against the effect of post-identification feedback in lineup identification. Post-identification feedback affects the recollection of participants and the eyewitness' confidence, but can be reduced by the use of suspicion. The application of suspicion to the legal system and as a bias correction is discussed.

Published
2006
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43. Normal Personality Variables and Their Relationship to Psychological Reactance.

Author

Seemann, Eric A., Buboltz, Walter C., Thomas, Adrian, Soper, Barlow, and Wilkinson, Lamar

Subjects
*PERSONALITY, *EMOTIONS, *MAN-woman relationships, *INTERPERSONAL relations, *ATTITUDE (Psychology), *BEHAVIOR
Abstract

This study examines the relationship between psychological reactance as measured by the Therapeutic Reactance Scale (TRS) and the five factor model of personality as measured by the NEO PI-R. The sample consisted of 310 undergraduate students at a mid-sized southern university. The TRS total score was regressed on the five domains of the NEO with three domains, Agreeableness, Extraversion, and Openness to Experience, retained in the solution. Two facets of Agreeableness (Straightforwardness and Compliance), one facet of Openness (Ideas), and four facets of Extraversion (Warmth, Assertiveness, Excitement-Seeking, and Positive Emotions) were significant. The significant domains and facets indicate that a reactant individual is likely to be controlling, territorial, possessive, suspicious, traditional, assertive, and moody. These results support previous findings with respect to psychological reactance and normal personality. Discussion includes speculation of types of reactant behavior as aspects of the reactance construct inferred by prior work. Study limitations and suggestions for future research are discussed. [ABSTRACT FROM AUTHOR]

Published
2005

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