1. Integrative multi-omics analysis reveals the underlying toxicological mechanisms of enrofloxacin on the growth of wheat seedling roots.
- Author
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Wu X, Chen X, Zhang D, Hu X, Ding W, Wang Y, Li G, Dong N, Hu H, Hu T, and Ru Z
- Subjects
- Oxidative Stress drug effects, Glutathione metabolism, Anti-Bacterial Agents toxicity, Proteomics, Plant Proteins metabolism, Metabolomics, Multiomics, Triticum drug effects, Triticum growth & development, Triticum metabolism, Seedlings drug effects, Seedlings growth & development, Seedlings metabolism, Enrofloxacin toxicity, Plant Roots drug effects, Plant Roots growth & development, Plant Roots metabolism, Reactive Oxygen Species metabolism
- Abstract
The continuous release of antibiotics into agroecosystems has raised concerns about the potential negative effects of antibiotic residues on crops. In this study, the toxicological effects of enrofloxacin (ENR) on wheat seedlings were analyzed using a combination of morpho-physiological, transcriptomic, proteomic, and metabolomic approaches. ENR inhibited the growth of wheat (Triticum aestivum L.) roots and induced oxidative stress. In particular, ENR downregulated the oxidative phosphorylation pathway, while it enhanced glycolysis and the tricarboxylic acid cycle, thereby regulating the balance of intracellular energy metabolism. In addition, sustained exposure to excessive reactive oxygen species (ROS) resulted in an increase in reduced glutathione (GSH), a slight decrease in ascorbic acid (AsA), and a significant decrease in the ratio of GSH to oxidized glutathione (GSSG), which imbalanced the AsA-GSH cycle. In addition, the resulting increase in abnormal proteins triggered ubiquitin-independent proteasomal degradation pathways. Further, an increase in abscisic acid (ABA) and a decrease in jasmonic acid (JA) and its derivatives alleviated the inhibitory effect of ENR on the growth of wheat roots. In conclusion, direct damage and signaling by ROS, hormonal regulation, a decrease in the GSH to GSSG ratio, and insufficient energy supply were identified as key factors for the significant inhibition of wheat root growth under ENR stress., Competing Interests: Declaration of Competing Interest The authors state that no known competing financial interest or personal relationship may have had any influence on any of the work disclosed in this study., (Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)
- Published
- 2024
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