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In this study, acetic acid bacteria (AAB) strains Ha5 T , Ta1 and Jb2 that constitute the core microbiota of weaver ant Oecophylla smaragdina were isolated from multiple ant colonies and were distinguished as different strains by matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry and distinctive random-amplified polymorphic DNA (RAPD) fingerprints. These strains showed similar phenotypic characteristics and were considered a single species by multiple delineation indexes. 16S rRNA gene sequence-based phylogenetic analysis and phylogenomic analysis based on 96 core genes placed the strains in a distinct lineage in family Acetobacteraceae . Compared to Acetobacteraceae type members, these strains demonstrate average nucleotide identity (ANI), in-silico DNA-DNA hybridization (DDH) and average amino acid identity (AAI) values lower than proposed species-level cut-off values which indicated that they represent a novel genus of the family. Currently, strains Ha5 T , Ta1 and Jb2 possess the smallest genomes (1.92-1.95 Mb) with significantly lower gene and protein numbers among family Acetobacteraceae . Intriguingly, the sole rrn operon in their genomes is plasmid-borne instead of chromosomally located. Furthermore, these strains harbour biosynthetic genes for various amino acids, cofactors and vitamins which supported a nutritional symbiotic interaction with the host O. smaragdina . Various phenotypic differences also distinguished the strains from closest relative genera in family Acetobacteraceae . Based on these results, these strains represent a novel species of a novel genus of family Acetobacteraceae , for which we propose the name Oecophyllibacter saccharovorans gen. nov. sp. nov., and strain Ha5 T as the type strain. Importance Acetic acid bacteria (AAB) of the family Acetobacteraceae are increasingly reported as symbionts of a wide range of insects but cultivation-based study to explore their functional role in the host remains limited. The weaver ant Oecophylla smaragdina is an effective biological control agent in pest management which harbours AAB as their core microbiota members. In this study, the cultivation and physiological characterization of Oecophyllibacter saccharovorans gen. nov. sp. nov. strains from the guts of O. smaragdina revealed the free-living capability of insect-associated AAB. These strains possess the smallest genomes with significantly lesser genes and proteins among Acetobacteraceae type members, which could be attributed to genome reduction. They also represent one of the rare examples with plasmid-borne sole rrn operon in their genome that might serve to compensate their low gene and protein number. Biosynthetic genes in the genomes provide insight into their biological function as symbiont of O. smaragdina .

DESCRIPTION OF PLANOCOCCUS VERSUTUS SP. NOV. Planococcus versutus (ver.su��� tus. L. masc. adj. versutus adroit, shrewd, ingenious). Cells are aerobic, Gram-stain-positive, motile and nonsporulating cocci. Colonies on LB agar are orange, circular, entire, smooth, convex and 1.0���2.0 mm in diameter. Grows at between 4 �� C and 30 �� C (optimum, 25 �� C) and at pH 6.0��� 11.0 (optimum, pH 7.0���8.0). Growth is observed between 0% and 14 % (w/v) NaCl (optimum, 6 %). Positive for catalase but negative for amylase. Positive for assimilation of N -acetyl- D- glucosamine, N -acetyl neuraminic acid, Oi -D- glucose, inosine, D- mannitol, glycerol, D- fructose 6-phosphate, glycyl L- proline, L- alanine, L- aspartic acid, L- glutamic acid, L- pyroglutamic acid, L- serine, L- galactonic acid lactone, D- gluconic acid, D- glucuronic acid, mucic acid, D- saccharic acid, D- lactic acid methyl ester, Oi -ketoglutaric acid, D- malic acid, L- malic acid, Tween 40, �� -hydroxy-DL- butyric acid, acetoacetic acid, acetic acid, formic acid, dextrin, D- fructose, D- glucose 6-phosphate, L- alanine, L- glutamic acid, pectin, D- galacturonic acid, glucuronamide and dextrin. Negative for assimilation of turanose, stachyose, D- mannose, 3- methyl glucose, D- sorbitol, citric acid, bromosuccinic acid, N -acetyl- �� -D- mannosamine, N -acetyl-D- galactosamine, D- galactose, D- fucose, L- fucose, L- rhamnose, D- arabitol, myo - inositol, D- aspartic acid, D- serine, gelatin, L- arginine, L- histidine, quinic acid, p -hydroxy-phenylacetic acid, methyl pyruvate, L- lactic acid, �� -aminobutryric acid, Oi - hydroxybutyric acid, Oi -ketobutyric acid and propionic acid. In chemical sensitivity tests, resistant to D- serine, lincomycin, guanidine HCl, tetrazolium blue, potassium tellurite, 1 % sodium lactate, aztreonam and sodium butyrate, slightly resistant to tetrazolium violet and sodium bromate, and sensitive to fusidic acid, nalidixic acid, lithium chloride, vancomycin, niaproof 4, troleandomycin, rifamycin SV and minocycline. The respiratory menaquinones are MK-5, MK-6 and MK-7. Major fatty acids are anteiso-C, C 16: 1 �� 7 c and anteiso-C. The predominant polar lipids are phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and aminophospholipids. 15: 0 17: 0 The type strain, L10.15 T (= DSM 101994 T = KACC 18918 T), was isolated from a soil sample collected from an elephant seal wallow in Lagoon Island (close to Adelaide Island, western Antarctic Peninsula). The DNA G+C content of the type strain is 39.4 mol%., Published as part of See-Too, Wah-Seng, Ee, Robson, Madhaiyan, Munusamy, Kwon, Soon-Wo, Tan, Jia Yi, Lim, Yan Lue, Convey, Peter, Pearce, David A., Yin, Wai Fong & Chan, Kok-Gan, 2017, Planococcus versutus sp. nov., isolated from soil, pp. 944-950 in International Journal of Systematic and Evolutionary Microbiology 67 (4) on pages 949-950, DOI: 10.1099/ijsem.0.001721, http://zenodo.org/record/6223682

12 páginas, 4 tablas y 5 figuras, A bacterial strain designated as P08T was isolated from laboratory tap water during a water quality assessment in University of Malaya, Malaysia. The strain was a Gramnegative, rod‐shaped, nonmotile, and aerobic bacterium. Complete genome of P08T comprised of a 2,820,660 bp chromosome with a G + C content of 36.43%. Both 16S rRNA phylogeny and phylogenetic tree inferred from the core gene matrix demonstrated that P08T formed a hitherto unknown subline within the family Neisseriaceae. Ortho average nucleotide identity (OrthoANI) values and the percentage of conserved proteins (POCP) calculated from complete genome sequence indicated low relatedness between P08T and its phylogenetic neighbors. Respiratory quinone analysis revealed Q‐8 as the only detectable quinone. The predominant cellular fatty acids were identified as C14:0, iso‐C15:0, and summed feature 3 (C16:1 ω7c/C16:1 ω6c). The polar lipids consisted of uncharacterized aminolipid, phosphatidylglycerol, and phosphatidylethanolamine. All aspects of phenotypic and phylogenetic data suggested that strain P08T represents a novel genus within family Neisseriaceae, for which the name Aquella gen. nov. is proposed. The type species of the genus is Aquella oligotrophica sp. nov., and the type strain is P08T (=LMG 29629T =DSM 100970T)., This work was supported by University of Malaya—Ministry of Higher Education High Impact Research Grant (UM‐MOHE HIR Grant UM.C/625/1/HIR/MOHE/CHAN/14/1, Grant No. H‐ 50001‐ A000027) and University of Malaya Research Grants (GA001‐2016, GA002‐2016) awarded to Kok‐Gan Chan and Postgraduate Research Grant (PPP) (Grant No. PG124‐2016A) awarded to Li Sin Lee. Kian Mau Goh thanks UTM GUP grant 15H50. Wah‐Seng See‐Too thanks Bright Sparks Unit of University of Malaya for the scholarship support.