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Microbes are prevalent in wastewater, degrading organic matter that impact the environment. In recent years, wastewater treatment plants have been used to process treated water for domestic purposes, sustaining groundwater resources for the future. The current study assessed the present microbial species in the aeration tanks for the secondary wastewater treatment stage in the Sulaibiya Wastewater Treatment and Reclamation Plant, Kuwait. Biweekly wastewater samples were collected for a period of three months from October 2022 to December 2022 from the aeration tanks. The collected wastewater samples were analyzed for microbiological parameters including total coliform, fecal coliform, Salmonella, and E. coli. Further, the microbial species were identified using the Biolog Omnilog Instrument. The results indicated that a total of 45 bacterial species isolates were identified with the most dominant genus being Bacillus with twenty-one isolates. Though Bacillus is common in aeration tanks, the presence of Bacillus thuringiens/cereus is rare and their existence could be due to the change in climatic conditions such as rainfall, dust and wind. Therefore, the study recommends that all microbial groups should be identified at all treatment stages including, primary, secondary, tertiary, and advanced treatment stages to ensure the complete removal of microbes. [ABSTRACT FROM AUTHOR]

Over the past two decades, interest in pollutant removal by yeasts has grown substantially. Yeasts can remove high amounts of pollutants at low production costs under non-sterile conditions. This work presents a compilation of the studies carried out regarding the potential application of yeasts in the treatment of wastewater. For example, a summary is presented on data about various yeast strains that are used to treat wastewater. The study will help the decision-making process for the selection of yeast for a type of wastewater and support research efforts by acquiring an overview of advancements in this area. Yeast treatment is versatile and has outstanding adaptability to varying treatment conditions. The effectiveness of yeast in treating wastewater is influenced by multiple factors. Yeast technology could potentially be retrofitted to existing activated sludge processes or be used instead of bacteria. Within its characteristics, we can observe tolerance to low pH (3.0–5.0), high salinity, high organic loads, antibiotics, and survive in up to 12% v/v alcohol mixtures. In fact, using low pH for yeast cultivation reduces bacterial contamination and supports yeast domination under non-sterile conditions. Laboratory-scale trials for yeast wastewater treatment have shown improvement over the past two decades; however, efficiencies differ according to the type of wastewater. In general, yeast offers several benefits compared to traditional microbial treatment methods, especially in its capacity to effectively process diverse organic carbon sources. However, it still must be proven to be an effective technology at an industrial scale.How to cite: Mohiuddin O, Harvey A, Orta Ledesma MT, et al. Bioremediation of waste by yeast strains. Electron J Biotechnol 2024;69. https://doi.org/10.1016/j.ejbt.2024.01.005.

Over the past two decades, interest in pollutant removal by yeasts has grown substantially. Yeasts can remove high amounts of pollutants at low production costs under non-sterile conditions. This work presents a compilation of the studies carried out regarding the potential application of yeasts in the treatment of wastewater. For example, a summary is presented on data about various yeast strains that are used to treat wastewater. The study will help the decision-making process for the selection of yeast for a type of wastewater and support research efforts by acquiring an overview of advancements in this area. Yeast treatment is versatile and has outstanding adaptability to varying treatment conditions. The effectiveness of yeast in treating wastewater is influenced by multiple factors. Yeast technology could potentially be retrofitted to existing activated sludge processes or be used instead of bacteria. Within its characteristics, we can observe tolerance to low pH (3.0-5.0), high salinity, high organic loads, antibiotics, and survive in up to 12% v/v alcohol mixtures. In fact, using low pH for yeast cultivation reduces bacterial contamination and supports yeast domination under non-sterile conditions. Laboratory-scale trials for yeast wastewater treatment have shown improvement over the past two decades; however, efficiencies differ according to the type of wastewater. In general, yeast offers several benefits compared to traditional microbial treatment methods, especially in its capacity to effectively process diverse organic carbon sources. However, it still must be proven to be an effective technology at an industrial scale. [ABSTRACT FROM AUTHOR]

The recent SARS‐CoV‐2 outbreak yielded substantial data regarding virus fate and prevalence at water reclamation facilities (WRFs), identifying influential factors as natural decay, adsorption, light, pH, salinity, and antagonistic microorganisms. However, no studies have quantified the impact of these factors in full scale WRFs. Utilizing a mass balance approach, we assessed the impact of natural decay and other fate mechanisms on genetic marker removal during water reclamation, through the use of sludge and wastewater genetic marker loading estimates. Results indicated negligible removal of genetic markers during P/PT (primary effluent (PE) p value: 0.267; preliminary and primary treatment (P/PT) accumulation p value: 0.904; and thickened primary sludge (TPS) p value: 0.076) indicating no contribution of natural decay and other fate mechanisms toward removal in P/PT. Comparably, adsorption and decomposition was found to be the dominant pathway for genetic marker removal (thickened waste activated sludge (TWAS) log loading 9.75 log10 GC/day); however, no estimation of log genetic marker accumulation could be carried out due to high detections in TWAS. Practitioner Points: The mass balance approach suggested that the contribution of natural decay and other fate mechanisms to virus removal during wastewater treatment are negligible compared with adsorption and decomposition in P/PT (p value: 0.904).During (P/PT), a higher viral load remained in the (PE) (14.16 log10 GC/day) compared with TPS (13.83 log10 GC/day); however, no statistical difference was observed (p value: 0.280) indicting that adsorption/decomposition most probably did not occur.In secondary treatment (ST), viral genetic markers in TWAS were consistently detected (13.41 log10 GC/day) compared with secondary effluent (SE), indicating that longer HRT and the potential presence of extracellular polymeric substance‐containing enriched biomass enabled adsorption/decomposition.Estimations of total solids and volatile solids for TPS and TWAS indicated that adsorption affinity was different between solids sampling locations (p value: <0.0001). [ABSTRACT FROM AUTHOR]

PURPOSEThe purpose of this study was to analyze and compare the outcomes of percutaneous microwave ablation (MWA) when used as a primary vs. secondary treatment for hepatocellular carcinoma (HCC).METHODSThe clinical data of 192 patients with HCC treated with MWA between January 2012 and July 2021 were reviewed retrospectively, with 152 patients being treatment naïve (primary treatment) vs. 40 who had residual or recurrent disease following previous trans-arterial chemoembolization or trans-arterial radioembolization (secondary treatment). The primary outcomes were primary technical efficacy, 1- and 3-year local recurrence-free survival (RFS) and overall survival (OS), local recurrence rates, and adverse events. Pre- and post-intervention liver function tests were compared using a Wilcoxon signed rank test. Univariate and multivariate analyses were also performed, looking at prognostic factors associated with OS and local RFS.RESULTSThere was no significant difference in 1-year local RFS (primary 93.6% vs. secondary 93.7; P = 0.97) and 3-year local RFS (primary 80.6% vs. secondary 86.5%; P = 0.37) rates. There was no significant difference in 1-year OS (primary 82.4% vs. secondary 86.6%; P = 0.51) and 3-year OS (primary 68.3% vs. secondary 77.4%; P = 0.25) between the two groups. The local recurrence rate (primary 9.8% vs. secondary 14.6%; P = 0.37), primary technical efficacy (primary 96.2% vs. secondary 95%; P = 0.73), and adverse events (primary 8.0% vs. secondary 11.6%; P = 0.45) were also similar between the two groups.CONCLUSIONMicrowave ablation is safe and effective as a secondary treatment for patients with HCC in a clinical salvage scenario and should be utilized more frequently.

Abstract Exponential increase in population has led to the release of large amounts of unregulated and untreated wastewater into water bodies such as rivers, lakes, streams, and estuaries causing various environmental and health issues. Traditionally various procedures are applied for the treatment of wastewater at the local and municipality level. Due to the increased need and development of newer technologies, it is necessary to understand all the available procedures and evaluate their efficiency. In the present review, all the important methods involved in the treatment of wastewater and its monitoring have been comprehensively discussed and compared along with their advantages and disadvantages. Information will not only help in understanding the treatment of wastewater but it will also help in developing procedures for the reuse of treated water for various alternative purposes.

PURPOSE The purpose of this study was to analyze and compare the outcomes of percutaneous microwave ablation (MWA) when used as a primary vs. secondary treatment for hepatocellular carcinoma (HCC). METHODS The clinical data of 192 patients with HCC treated with MWA between January 2012 and July 2021 were reviewed retrospectively, with 152 patients being treatment naive (primary treatment) vs. 40 who had residual or recurrent disease following previous trans-arterial chemoembolization or trans-arterial radioembolization (secondary treatment). The primary outcomes were primary technical efficacy, 1- and 3-year local recurrence-free survival (RFS) and overall survival (OS), local recurrence rates, and adverse events. Pre- and post-intervention liver function tests were compared using a Wilcoxon signed rank test. Univariate and multivariate analyses were also performed, looking at prognostic factors associated with OS and local RFS. RESULTS There was no significant difference in 1-year local RFS (primary 93.6% vs. secondary 93.7; P = 0.97) and 3-year local RFS (primary 80.6% vs. secondary 86.5%; P = 0.37) rates. There was no significant difference in 1-year OS (primary 82.4% vs. secondary 86.6%; P = 0.51) and 3-year OS (primary 68.3% vs. secondary 77.4%; P = 0.25) between the two groups. The local recurrence rate (primary 9.8% vs. secondary 14.6%; P = 0.37), primary technical efficacy (primary 96.2% vs. secondary 95%; P = 0.73), and adverse events (primary 8.0% vs. secondary 11.6%; P = 0.45) were also similar between the two groups. CONCLUSION Microwave ablation is safe and effective as a secondary treatment for patients with HCC in a clinical salvage scenario and should be utilized more frequently. [ABSTRACT FROM AUTHOR]

Purpose: With increasing patient self-empowerment and participation in decision making, we hypothesized that patients with adult-type diffuse gliomas, CNS WHO grade 4 who change sites of treatment differ from patients being entirely treated in one neuro-oncological center. Methods: Prospectively collected data from all diffuse glioma grade 4 patients who underwent treatment in our neuro-oncological center between 2012 and 2018 were retrospectively examined for differences between patients having initially been diagnosed and/or treated elsewhere (External Group) and patients having entirely been treated in our neuro-oncological center (Internal Group). Additionally, a matched-pair analysis was performed to adjust for possible confounders. Results: A total of 616 patients was analyzed. Patients from the External Group (n = 78) were significantly younger, more frequently suffered from IDH-mutant astrocytoma grade 4, had a greater extent of tumor resection, more frequently underwent adjuvant therapy and experienced longer overall survival (all p < 0.001). However, after matching these patients to patients of the Internal Group considering IDH mutations, extent of resection, adjuvant therapy, age and gender, no difference in patients' overall survival was observed anymore. Conclusion: The present study demonstrates that mobile diffuse glioma grade 4 patients stand out from a comprehensive diffuse glioma grade 4 patient cohort due to their favorable prognostic characteristics. However, changing treatment sites did not result in survival benefit over similar patients being entirely taken care of within one neuro-oncological institution. These results underline the importance of treatment and molecular markers in glioma disease for patients' self-empowerment, including changing treatment sites according to patients' needs and wishes. [ABSTRACT FROM AUTHOR]

Aim- The aim of this research is to compare suspended growth reactors MBR and SBR to calculate the organic content reduction efficacy offered by the reactors for different concentrations of wastewater from the dairy industry. Materials and methods - The secondary treatment reactors, MBR and SBR having an average Organic Loading Rate (OLR) of 0.55 g/m3/d to 1.11 g/m3/d, were compared for their Biological Oxygen Demand (BOD5) removal efficacy for a period of 5 consecutive days for wastewater effluents from dairy industries such as mixed dairy, cheese, cheese whey, milk permeate, butter, dairy sewage, ice cream. Test samples (N = 14) were taken from the outfall of the experimental group (MBR) and control group (SBR) using G Power software. G power is taken as 0.8. Results- The performance of an individual MBR reactor at removing the BOD5 content was 95% compared to an individual SBR reactor which showed only 65%. On the other hand, the treatment plant as a whole which employed MBR reactor showed a removal efficacy of 84% and the plant which employed SBR reactor showed only 70% removal efficacy of BOD5. The significance value is determined as 0.04 (p is less than 0.027, statistically significant) based on SPSS analysis. Conclusion - MBR not only showed a better efficacy at removing BOD5 from the dairy wastewater but was also cost efficient ($7.59) than SBR ($11.53), the only downside to the MBR reactor was that it utilised more power of 62.82 kW than that of SBR (44.71 kW). MBR showed a lesser efficacy of 52% for treating the effluents from ice cream which had an OLR of 0.156 g/m3/d and the reactor showed its highest efficacy of 99.8 % for treating milk permeate effluents of having an OLR of 1.73 g/m3/d. [ABSTRACT FROM AUTHOR]

To cope with water scarcity, Morocco needs to integrate the reuse of treated wastewater fully into its water management strategies. However, this option imposes several concerns about the appropriate wastewater treatment system (WWTS) whose performance is balanced by technical, environmental, financial, and societal sustainability. To deal with these challenges, the present study aims to assess the sustainability of five WWTS using the cardinal (CAR) and PROMETHEE methods. After hierarchizing the criteria and identifying the WWTS, two separate surveys were performed in order to rank criteria and alternatives by preference strength. The delivered rankings were converted, then, respectively into cardinal weights (criteria) and cardinal scores (alternatives). The PROMETHEE rankings showed that the membrane systems are the most sustainable followed by trickling filters, while the infiltration-percolation is the least sustainable WWTS. The activated sludge and lagoon systems were incomparable using PROMETHEE I partial ranking, and they were both ranked in the third position of sustainability. In contrast, PROMETHEE II complete ranking favored the activated sludge than lagoon systems due to its slightly high net outranking flow. The stability intervals indicated that the weights of all criteria could not affect the two first actions, which is mainly due to the high precision and robustness of the CAR method in eliciting weights. Finally, each criterion affected variably the sustainability of WWTS according to their characteristics, but overall, the process efficiency is the key factor (21.07% of weight) to reaching higher sustainability levels in addition to gaseous emissions (12.41% of weight), flexibility (8.32% of weight), and energy requirement (7.50% of weight). [ABSTRACT FROM AUTHOR]

Microplastic (MP) water pollution is a major problem that the world is currently facing, and wastewater treatment plants (WWTPs) represent one of the main alternatives to reduce the MP release to the environment. Several studies have analysed punctual samples taken throughout the wastewater treatment line. However, there are few long-term studies on the evolution of MPs over time in WWTPs. This work analyses the performance of a WWTP sited in Southwest Europe in relation with annual occurrence and fate of MPs. Samples were monthly taken at different points of the facility (influent, secondary effluent, final effluent, and sludge) and MPs were quantified and characterised by means of stereomicroscopy and FTIR spectrophotometry. The majority of MPs found in wastewater and sludge samples were fragments and fibres. Regarding to the chemical composition, in the water samples, polyethylene (PE), polyethylene terephthalate (PET) and polypropylene (PP) stood out, whereas, in the sludge samples, the main polymers were PET, polyamide (PA) and polystyrene (PS). The MPs more easily removed during the wastewater treatment processes were those with sizes greater than 500 µm. Results showed that the MPs removal was very high during all the period analysed with removal efficiencies between 89% and 95%, so no great variations were found between months. MP concentrations in dry sludge samples ranged between 12 and 39 MPs/g, which represented around 79% of the total MPs removed during the wastewater treatment processes. It is noticeable that a trend between temperature and MPs entrapped in sewage sludge was observed, i.e., higher temperatures entailed higher percentage of retention. [ABSTRACT FROM AUTHOR]

Data supporting the use of etoposide-based therapy in hemophagocytic lymphohistiocytosis (HLH) arise largely from pediatric studies. There is a lack of comparable data among adult patients with secondary HLH. We conducted a retrospective study to assess the impact of etoposide-based therapy on outcomes in adult secondary HLH. The primary outcome was overall survival. The log-rank test was used to compare Kaplan-Meier distributions of time-to-event outcomes. Multivariable Cox proportional hazards modeling was used to estimate adjusted hazard ratios (HRs) with 95% confidence intervals (CIs). Ninety adults with secondary HLH seen between January 1, 2009, and January 6, 2020, were included. Forty-two patients (47%) received etoposide-based therapy, while 48 (53%) received treatment only for their inciting proinflammatory condition. Thirty-three patients in the etoposide group (72%) and 32 in the no-etoposide group (67%) died during follow-up. Median survival in the etoposide and no-etoposide groups was 1.04 and 1.39 months, respectively. There was no significant difference in survival between the etoposide and no-etoposide groups (log-rank p = 0.4146). On multivariable analysis, there was no association between treatment with etoposide and survival (HR for death with etoposide = 1.067, 95% CI: 0.633–1.799, p = 0.8084). Use of etoposide-based therapy was not associated with improvement in outcomes in this large cohort of adult secondary HLH patients. [ABSTRACT FROM AUTHOR]

The first Italian annual monitoring study was carried out in Northern Italy to analyse the fate and removal of polycyclic musk fragrances (PMFs) in a wastewater treatment plant (WWTP) with conventional activated sludge (CAS) system. Water was sampled in four different stations along wastewater treatments to better understand the behaviour of PMFs along different steps of the plant. Galaxolide (HHCB) and galaxolidone (HHCB-lactone) were found in concentrations at μg L−1 level, 1 order of magnitude greater than tonalide (AHTN), whilst phantolide (AHDI) was never detected and celestolide (ADBI) was measured only at trace levels. Considering water concentrations, HHCB and AHTN evidenced a slight reduction, 20% and 50%, respectively, during wastewater treatments, thus resulting in a modest removal efficiency, mainly due to adsorption processes during the biological treatment. This was also confirmed by the high PMF concentrations measured in activated sludges which remained stable throughout the year. On the contrary, HHCB-lactone registered an increase up to 70% during wastewater treatments caused by the biotransformation of the parental compound HHCB during the biological treatment, as shown by the different HHCB-lactone/HHCB ratio measured before and after this step. No significant differences were recorded between seasons in terms of PMF input onto WWTP, in accordance with the common use of these chemicals in civil houses. Overall, this study suggests that current technologies employed in conventional WWTP are not enough efficient in removing these organic micropollutants from wastewaters and, therefore, WWTP effluents represent possible point sources of pollution for aquatic ecosystems. Additional treatments are necessary to enhance the removal of PMFs in order to increase the quality of the WWTP effluents. [ABSTRACT FROM AUTHOR]

The various treatment steps required to get the complete textile ZLD ETP were already discussed. Primary, secondary and tertiary treatments followed by advancedfiltration are the important steps of ETP. Understanding the principles of each process, effluent retention time in each step, and the necessity ofproper sizing of equipment are the main key factors and performance assuring things in ETP. Thefiltration system and TDS mass balance along with permeate water quality is discussed with facts andfigures. [ABSTRACT FROM AUTHOR]

Concern for overtreatment in very low-, low-, and favorable intermediate-risk prostate cancer has promoted a more conservative approach through active surveillance (AS) with comparable survival outcomes. We analyzed the National Cancer Database (NCDB) to determine if delaying radical prostatectomy greater than 6 months is associated with an increase in the rate of adverse pathology or secondary treatment (adjuvant or salvage) at radical prostatectomy. Utilizing the NCDB from 2004 to 2019, 40 to 75-year-old men with very low-, low-, and favorable-intermediate-risk prostate cancer, as defined by the National Comprehensive Cancer Network, were identified for this study. These individuals received radical prostatectomy either before or after 6 months following diagnosis. Clinical, demographic, and pathologic characteristics were obtained. Adverse pathologic outcomes were defined as pT3-4N0-1 and/or positive surgical margins. Multiple logistic regression models were used to predict delays in treatment, adverse pathologic outcomes, and receipt of secondary therapy. Survival analysis was performed using the Cox Proportional Hazards Model and the Kaplan-Meier Method. Of the 195,397 patients who met inclusion criteria, only 13,393 patients received surgery 6 months after diagnosis. The median time of delay was 7.5 months compared to 2.3 months in the immediate treatment group. Overall, delaying surgery had no statistically significant impact on adverse pathologic outcomes, regardless of risk category. However, when accounting for the interaction between race and delayed treatment, non-Hispanic black patients who received a delay in treatment were more likely to experience adverse features (OR 1.12, 95%CI 1.00-1.26, P =.041). Conversely, patients who had delayed surgery were less likely to receive additional therapy (either adjuvant or salvage) (OR 0.60, 95%CI 0.52-0.68, P <.001). Survival analysis showed that both groups fared well, with a 5-year survival of 97% for both groups. The treatment group was not predictive of survival. Overall, delaying surgery more than 6 months following diagnosis did not have a significant impact on adverse pathologic features or overall survival. However, when specifically looking at non-Hispanic black patients with a treatment delay, these patients were at increased risk for adverse features, suggesting that the negative impact of treatment delay depends on the patient's race. As race is a social construct, this finding likely points to the complex socioeconomic factors that contribute to overall health outcomes rather than any inherent disease characteristics. Lastly, delayed treatment patients were actually less likely to require secondary therapy, regardless of race, possibly reflecting high clinician acumen in selecting patients appropriate for treatment delay. The results suggest that patients who ultimately "fail" AS and require subsequent surgery have overall comparable survival outcomes. However, pathologic outcomes are dependent on the patient's underlying race, with non-Hispanic black patients experiencing an increased risk of adverse outcomes if treatment is delayed. Delaying surgery more than 6 months following diagnosis showed no impact on adverse pathologic outcomes or overall survival. These patients were also less likely to require secondary therapy. The results suggest that even patients who "fail" AS and require subsequent surgery have comparable positive outcomes to those who receive immediate treatment. [ABSTRACT FROM AUTHOR]

We investigated the causative factors of abdominal support and shape and found that excessive musculoskeletal flaccidity of primary origin causes an inability to support the abdominal wall and may be associated with the predisposing factors. For such cases, we developed a treatment consisting of the placement of a subcutaneous mesh. Here, we present our experience with this treatment. We present a case series of 15 patients in our 26 years of experience who were treated with primary and secondary abdominoplasties. The abdominal wall was reinforced by placing a polypropylene mesh in the submuscular plane with U-stitches in the transversalis fascia, aiming at strengthening the muscle and transverse fascia. The results were satisfactory in the long term. Abdominal bulges were repaired, and muscle harmony was restored. Only two complications occurred: chronic pain localized in the abdomen, which was treated with steroid infiltrations, and an early umbilical fistula with spontaneous and rapid resolution, regardless of the proposal.

Microplastic (MP) water pollution is a major problem that the world is currently facing, and wastewater treatment plants (WWTPs) represent one of the main alternatives to reduce the MP release to the environment. Several studies have analysed punctual samples taken throughout the wastewater treatment line. However, there are few long-term studies on the evolution of MPs over time in WWTPs. This work analyses the performance of a WWTP sited in Southwest Europe in relation with annual occurrence and fate of MPs. Samples were monthly taken at different points of the facility (influent, secondary effluent, final effluent, and sludge) and MPs were quantified and characterised by means of stereomicroscopy and FTIR spectrophotometry. The majority of MPs found in wastewater and sludge samples were fragments and fibres. Regarding to the chemical composition, in the water samples, polyethylene (PE), polyethylene terephthalate (PET) and polypropylene (PP) stood out, whereas, in the sludge samples, the main polymers were PET, polyamide (PA) and polystyrene (PS). The MPs more easily removed during the wastewater treatment processes were those with sizes greater than 500 µm. Results showed that the MPs removal was very high during all the period analysed with removal efficiencies between 89% and 95%, so no great variations were found between months. MP concentrations in dry sludge samples ranged between 12 and 39 MPs/g, which represented around 79% of the total MPs removed during the wastewater treatment processes. It is noticeable that a trend between temperature and MPs entrapped in sewage sludge was observed, i.e., higher temperatures entailed higher percentage of retention.

The concentrations of six heavy metal/metalloids (HMs) cadmium (Cd), copper (Cu), mercury (Hg), lead (Pb), zinc (Zn), and arsenic (As) were determined in the influent, effluent, and dewatered sludge of two sewage treatment works (Yuen Long Sewage Treatment Work (YLSTW) and Shek Wu Hui Sewage Treatment Work (SWHSTW)) and river waters and sediment (Shan Pui River and Ng Tung River) within the Mai Po Ramsar site in Hong Kong SAR, China. In both STWs, Pb had the highest removal efficiencies (YLSTW 86.5 ± 19.0% to 97.3 ± 3.04%; SWHSTW 87.4 ± 12.8% to 100 ± 0.0800%). In the whole effluent toxicity test, both STWs were effective in lowering the toxicity of the effluent to zebrafish and cherry shrimp. The environmental risk assessments of these HMs on three local aquatic organisms, three species of fish, two species of crustaceans, and three species of algae, were calculated based on measured HMs concentrations in river water and sewage samples, and the predicted no-effect concentrations. Results showed that Zn concentrations in all collected samples posed potential risks to all studied aquatic organisms (average risk quotient = 445). The concentrations of Cu in the rivers posed potential risks (average risk quotient = 5.42) to all fish species. It was concluded that Zn and Hg derived from the STWs might pose potential risks to the living organisms inhabiting the Ramsar site, and Cu, Cd, Pb, and As in the rivers were originated from the two rivers and possibly other tributaries. [ABSTRACT FROM AUTHOR]

Industrial development has increased wastewater (WW) volume; generating contamination and disturbing ecosystems, because of breeching disposal parameters. In this work, Coloured Laboratory Wastewater (CLWW), (1500.00 colour units, CU) was separately submitted to two secondary treatments. For the first one CLWW was treated for three cycles C1, C2 and C3 with P. pastoris X33/pGAPZαA-LaccPost-Stop producing rPOXA 1B laccase, immobilized in calcium alginate beads. For the second-one, rPOXA 1B enzyme concentrate was used (three processes: P1, P2, and P3). Both treatments were carried out in a 15 L reactor with 10 L effective work volume (EWV) with 72 h hydraulic retention time. C1, C2, and C3 effluents were flocculated and filtered through quartzite sand, while P1, P2, and P3 effluents were only filtered through quartzite sand. The mixture of secondary effluents was submitted to a tertiary treatment with Chlorella sp. For C1, C2, C3, P1, P2, and P3, CU removal was of 99.16, 99.58, 99.53, 96.72, 97.05 and 96.47%, respectively. Discharge parameters, total organic carbon (TOC), inorganic carbon (IC), chemical oxygen demand (COD) and biological oxygen demand (BOD5) decreased, although they reached different final values. After the tertiary treatment (144 h) effluent discharge parameters were reduced to 34 ± 4 CU, TOC to 6.6 ± 0.9 mg L−1 and COD to 155 ± 4 mg L−1. It was demonstrated that secondary treatments (immobilized recombined cells or recombinant enzyme concentrate) combined with Chlorella sp., (tertiary treatment) attained a considerable removal of discharge parameters, demonstrating a promissory alternative for CLWW sequential treatment. [ABSTRACT FROM AUTHOR]

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Research using anaerobic filters as alternative materials to gravel becomes fundamental, especially if they are low cost, accessible and have high efficiency in the treatment of swine wastewater. This study aimed to evaluate the efficiency of anaerobic filters with different types of support media, as an alternative to gravel, in the treatment of swine wastewater. We carried out the experiment in a completely randomized design. The treatments were composed of anaerobic filters with four different media (gravel, bamboo, luffa sponge and foam), three replications in a time-repeated measurements scheme (0, 60, 120, 180 and 240 days post-filter establishment). To determine the efficiency of removal by the filters, we performed analysis of turbidity, total solids, biochemical oxygen demand (bod), total n and total p in the affluent to and effluent from the filters. Among the alternative media, bamboo presented an efficiency of removal of the evaluated attributes similar and, in most cases, superior to gravel. From 60 to 180 days of filter operation, the luffa sponge remained with high turbidity and total solids removals, with the greatest decrease at 240 days of operation. After two months of filter operation, the alternative support media exhibited bod and total n removal similar to that of gravel. At 240 days of operation, the filters containing luffa sponge and bamboo showed the highest total p removal.

Neuroendocrine prostate cancer (NEPC) is a histological variant of prostate cancer and is characterized by aggressiveness and poor clinical outcomes. NEPC usually develops as a mechanism of treatment resistance in patients receiving hormone therapy for advanced prostate cancer. NEPC is sensitive to primary platinum-based chemotherapy, and has a short response duration. Second-line therapy is required in many cases, but clinical data on subsequent treatment after progression to first-line chemotherapy is limited. Here we report our experience of four cases of NEPC treated with second-line chemotherapy. Progression-free and overall survival rates were very low in three of the patients. One patient received multidisciplinary therapy using systemic and local chemotherapy and radiation therapy and survived for 24 months after initiation of second-line chemotherapy. Multidisciplinary therapy with chemotherapy and radiation is a promising option for improving the survival of patients with NEPC.

The effluent of secondary treatment process at Al-Quds University wastewater treatment plant was tertiary treated using an Epuvalisation technique in which geranium plants (Pelargonium hortorum) was employed as a treatment vehicle. The Epuvalisation system was installed in a greenhouse in which germanium was planted hydroponically in a closed-loop mode using secondary treated wastewater (TWW) and fresh water (FW) as a control. The water quality analysis results before and after the growing season of TWW showed a reduction of 23% and 41%, for BOD and COD, respectively. The electrical conductivity (EC) and total dissolved solids (TDS) for TWW demonstrated a reduction of 52% and 53%, respectively. Similar trend was observed for FW in which the reduction in EC and TDS were 48% and 49%, respectively. Furthermore, the percent reduction of the concentration of suspended solids, phosphate ions (PO 4 −3), chloride ion (Cl−), total nitrogen (TN), and potassium ion (K+) in TWW were found to be 95%, 89%, 60% 98% and 59%, respectively. Plant growth parameters (plant height, fresh and dry weight, number of branches and flowers number) of geranium revealed no significant difference between irrigation with TWW and FW. The chemical plant analysis of roots, leaves, stems and flowers revealed no significant difference between hydroponic plantation in TWW and in FW. From these results it is safe to conclude that the Epuvalisation technique is a promising technique for advanced wastewater treatment using ornamental plants such as geranium due to its simplicity, low cost and easy use. [ABSTRACT FROM AUTHOR]

• Cost for assimilation wetlands averaged $0.60/gallon of treatment capacity. • 2° and 3° treatment costs averaged $4.90 and $6.50/gallon, respectively. • Wetland assimilation is less sensitive to cost increases than traditional systems. In recent decades, water quality standards for wastewater treatment have become more stringent, increasing costs and energy required to reduce pollutants. Wetland assimilation is a low-cost and low-energy alternative to traditional tertiary wastewater treatment where secondarily treated and disinfected municipal effluent is discharged primarily into freshwater forested wetlands in coastal Louisiana. In this paper, costs per gallon of treatment capacity for conventional secondary and tertiary treatment were compared to those for assimilation wetlands. Cost analysis reports were used to determine costs per gallon of treatment capacity for conventional wastewater treatment facilities, including costs for conveyance between the collection system and the assimilation wetland site, and between the treatment and disposal sites if they could not be co-located. Capital and operation and maintenance costs were considered. Because all wastewater treatment plants are required to treat at least to secondary standards, costs for primary and secondary treatment were combined. If necessary, these costs were adjusted for inflation to 2017 dollars using an average inflation rate of 2.19 percent and a cumulative inflation rate of 50.84 percent. To determine costs per gallon of treatment capacity for assimilation wetlands, actual costs provided by the project engineer were used when available. To simulate the future costs of facility construction and compare the replacement costs of conventional secondary and tertiary wastewater treatment facilities and treatment wetlands in the context of energy prices, U.S. Bureau of Labor and Statistics (BLS) data for the price index for inputs to construction were used, as were the Energy Information Administration (EIA) data for the price of crude oil to model future wastewater treatment plant construction and operation costs. The cost for the Mandeville assimilation wetland included $1 million for the price of the land. Future costs of treatment facility construction and operation were modeled relative to average price of construction inputs between 1998 and 2015 using the projected price of crude oil. When treatment costs were compared among secondary, tertiary, and assimilation wetlands, mean cost for assimilation wetlands was $0.60 per gallon (>1 MGD capacity) compared to $4.90 and $6.50 per gallon for secondary and tertiary treatment, respectively. The lower total costs and energy requirements for assimilation wetlands result in lower variability in the price of construction and operation. Wetland assimilation is more economical than conventional wastewater treatment, especially compared to advanced secondary and tertiary treatment. It is likely that energy costs will increase significantly in coming decades. Because conventional secondary and tertiary treatment are energy intensive, increases in energy costs will significantly increase the costs of these treatment systems. Treatment systems that combine lower technology (e.g., oxidation ponds) secondary treatment with wetland assimilation are less likely to be impacted by rising energy costs than traditional wastewater treatment. [ABSTRACT FROM AUTHOR]

Background and Objective: Estrogen is a steroid hormone that is in the water and cause risks for aquatic organisms, especially fish is. Estrogenic hormones naturally (caused by endocrine) or artificial (due to hormonal contraceptives such as birth control pills) to sewage and hospital to find. This study aimed to determine the amount of estrogen in the sewage and Golestan and Abuzar hospital in Ahvaz were studied. Material and Methods: Sampling was done by tracking estrogen in the aquatic environment. 56 samples 4 months in different parts of the Golestan and Abuzar hospital wastewater treatment plant in Ahvaz were taken. Sample a little electro luminescence (ECL) were analyzed by laboratory Iranian city of Ahvaz. In this study, different forms of estrogen (estrone, 17-beta-estradiol, estriol, 17-alpha-ethinyl estradiol) has been studied as estrogen. Results: Mean hormone found in raw wastewater to the Golestan hospital wastewater treatment plant effluent from the treatment plant hormone 69.08 ng/l and 7.28 ng/l mean. Mean testosterone in Ahvaz Abuzar hospital treatment input 70.61 ng/l and 18.94 ng/l is refinery output. According to the results of high impact biological treatment is the removal of estrogenic compounds. Conclusion: Secondary treatment of sewage is effective in reducing hormones that can do this at this stage of the biological treatment effect.

Wastewater treatment plants (WWTPs) are the most common form of industrial and municipal wastewater control. To evaluate the performance of wastewater treatment and the potential risk of treated wastewater to aquatic life and human health, the influent and effluent concentrations of nine toxic metals were determined in 12 full-scale WWTPs in Shanghai, China. The performance was evaluated based on national standards for reclamation and aquatic criteria published by US EPA, and by comparison with other full-scale WWTPs in different countries. Potential sources of heavy metals were recognized using partial correlation analysis, hierarchical clustering, and principal component analysis (PCA). Results indicated significant treatment effect on As, Cd, Cr, Cu, Hg, Mn, Pb, and Zn. The removal efficiencies ranged from 92% (Cr) to 16.7% (Hg). The results indicated potential acute and/or chronic effect of Cu, Ni, Pb, and Zn on aquatic life and potential harmful effect of As and Mn on human health for the consumption of water and/or organism. The results of partial correlation analysis, hierarchical clustering based on cosine distance, and PCA, which were consistent with each other, suggested common source of Cd, Cr, Cu, and Pb and common source of As, Hg, Mn, Ni, and Zn. Hierarchical clustering based on Jaccard similarity suggested common source of Cd, Hg, and Ni, which was statistically proved by Fisher’s exact test. [ABSTRACT FROM AUTHOR]

To perform a systematic survey on the occurrence and removal of micropollutants during municipal wastewater treatment, 943 semi-volatile organic chemicals in 32 wastewater samples including influents of secondary treatments, secondary effluents and final effluents (effluents of advanced treatments), which were collected from seven full-scale municipal wastewater treatment plants (MWTPs) in China, were examined by gas chromatography-mass spectrometry (GC-MS) coupled with an automated identification and quantification system with a database (AIQS-DB). In total, 196 and 145 chemicals were detected in secondary and final effluents, respectively. The majority of the total concentrations (average removal efficiency, 87.0%±5.9%) of the micropollutants were removed during secondary treatments. However, advanced treatments achieved different micropollutant removal extents from secondary effluents depending on the different treatment processes employed. Highly variable removal efficiencies of total concentrations (32.7%–99.3%) were observed among the different advanced processes. Among them, ozonation-based processes could remove 70.0%–80.9% of the total concentrations of studied micropollutants. The potentially harmful micropollutants, based on their detection frequency and concentration in secondary and final effluents, were polycyclic aromatic hydrocarbons (PAHs) (2-methylnaphthalene, fluoranthene, pyrene, naphthalene and phenanthrene), phosphorus flame retardants (tributyl phosphate (TBP), tris(2-chloroethyl) phosphate (TCEP) and tris(1,3-dichloro-2-propyl) phosphate (TDCP)), phthalates (bis(2-ethylhexyl)phthalate (DEHP)), benzothiazoles (benzothiazole, 2-(methylthio)-benzothiazol, and 2(3H)-benzothiazolone) and phenol. This study indicated that the presence of considerable amounts of micropollutants in secondary effluent creates the need for suitable advanced treatment before their reuse. [ABSTRACT FROM AUTHOR]

This study aimed at investigating the removal efficiencies of perfluorooctanoate (PFOA), perfluorooctane sulfonate (PFOS) and bisphenol A (BPA) of two major sewage treatment works in Hong Kong: Stonecutters Island STW (SCISTW) which adopts chemically enhanced primary treatment (CEPT) process and Sha Tin STW (STSTW) which employs biological treatment. Liquid portion (LP) and particulate matter (PM) of liquid sewage samples and sludge samples were collected and followed by liquid chromatograph system. It was found that BPA (44.6 ± 35.1%) generally achieved higher TRE than the two other chemicals (PFOS (−18.8 ± 34.8%) and PFOA (−104 ± 86.8%)) in STSTW ( p < 0.05). Most of the PFOA, PFOS and BPA was discharged through final effluent (PFOA: 95.6 ± 1.00% and 94.5 ± 3.13%; PFOS: 77.7 ± 1.48% and 72.6 ± 6.07%; BPA: 99.2 ± 0.950% and 92.8 ± 7.25%, respectively) rather than stored in the sludge/cake (PFOA: 4.45 ± 1.00% and 5.47 ± 3.13%, PFOS: 22.3 ± 1.48% and 27.4 ± 6.07%, BPA: 0.844 ± 0.950% and 7.20 ± 7.25%, respectively). After the sewage purification process, the two STW released considerable amounts of PFOA, PFOS and BPA in the final effluent (PFOA: 0.638 ± 0.227 kg/year; PFOS: 0.409 ± 0.126 kg/year; BPA: 10.4 ± 3.83 kg/year in STSTW; PFOA: 3.08 ± 1.415 kg/year; PFOS: 2.13 ± 0.452 kg/year; BPA: 714 ± 768 kg/year in SCISTW) and in the sludge (PFOA: 0.0360 ± 0.0250 kg/year; PFOS: 0.149 ± 0.00100 kg/year; BPA: 1.09 ± 1.47 kg/year in STSTW; PFOA: 0.139 ± 0.0670 kg/year; PFOS: 0.606 ± 0.0780 kg/year; BPA: 3.05 ± 3.95 kg/year in SCISTW). This study may help to provide crucial information for further development of municipal sewage system in treating synthetic emerging chemicals. [ABSTRACT FROM AUTHOR]

The activated sludge (AS) process is the most common type of secondary wastewater treatment, applied worldwide. Due to the complexity of microbial communities, imbalances between the different types of bacteria may occur and disturb the process, with pronounced economical and environmental consequences. Microscopic inspection of the morphology of flocs and microorganisms provides key information on AS properties and function. This is a time-consuming, highly skilled, and expensive process that is not readily available in all locations. Thus, most wastewater-treatment plants do not carry out this essential analysis, resulting in frequent operational faults. In this study, we develop a novel deep learning (DL) object detection algorithm to analyze and monitor the AS process based on a unique microscopic image database of flocs and microorganisms. Specifically, we applied YOLOv5 and Faster R-CNN algorithms as tools for segmentation and object detection to analyze the wastewater. The mean average precision (mAP) of the YOLOv5 was 0.67, outperforming the Faster R-CNN by 15%. Histogram equalization preprocessing of both bright-field and phase-contrast images significantly improved the results of the algorithm in all classes. In the case of YOLOv5, the mAP increased by 16.67%, to 0.77, where the AP of protozoa, filaments, and open floc classes outperformed the previous model by over 20%. These results demonstrate the potential of leveraging DL algorithms to enhance the analysis and monitoring of WWTPs in an affordable manner, consequently reducing environmental pollution caused by contaminated effluent. The fundamental challenge addressed herein has important global relevance, especially in an era in which the demand for high-quality wastewater reuse is expected to increase dramatically. [Display omitted] • Deep learning object detection algorithms for secondary treatment are proposed. • Mean average precision of YOLOv5 was 0.67, outperforming Faster R-CNN by 15%. • Histogram equalization significantly improved mAP of YOLOv5 by 16.67% to 0.77 • Unique microscopic image database from Israeli WWTPs was used. • This framework can be adopted by WWTPs to manage secondary treatment process. [ABSTRACT FROM AUTHOR]