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Background: Virus‐like particle (VLP) Peanut is a novel immunotherapeutic vaccine candidate for the treatment of peanut allergy. The active pharmaceutical ingredient represents cucumber mosaic VLPs (CuMVTT‐VLPs) that are genetically fused with one of the major peanut allergens, Ara h 2 (CuMVTT‐Ara h 2). We previously demonstrated the immunogenicity and the protective capacity of VLP Peanut‐based immunization in a murine model for peanut allergy. Moreover, a Phase I clinical trial has been initiated using VLP Peanut material manufactured following a GMP‐compliant manufacturing process. Key product characterization studies were undertaken here to understand the role and contribution of critical quality attributes that translate as predictive markers of immunogenicity and protective efficacy for clinical vaccine development. Method: The role of prokaryotic RNA encapsulated within VLP Peanut on vaccine immunogenicity was assessed by producing a VLP Peanut batch with a reduced RNA content (VLP Peanut low RNA). Immunogenicity and peanut allergen challenge studies were conducted with VLP Peanut low RNA, as well as with VLP Peanut in WT and TLR 7 KO mice. Furthermore, mass spectrometry and SDS‐PAGE based methods were used to determine Ara h 2 antigen density on the surface of VLP Peanut particles. This methodology was subsequently applied to investigate the relationship between Ara h 2 antigen density and immunogenicity of VLP Peanut. Results: A TLR 7 dependent formation of Ara h 2 specific high‐avidity IgG antibodies, as well as a TLR 7 dependent change in the dominant IgG subclass, was observed following VLP Peanut vaccination, while total allergen‐specific IgG remained relatively unaffected. Consistently, a missing TLR 7 signal caused only a weak decrease in allergen tolerability after vaccination. In contrast, a reduced RNA content for VLP Peanut resulted in diminished total Ara h 2 specific IgG responses, followed by a significant impairment in peanut allergen tolerability. The discrepant effect on allergen tolerance caused by an absent TLR 7 signal versus a reduced RNA content is explained by the observation that VLP Peanut‐derived RNA not only stimulates TLR 7 but also TLR 3. Additionally, a strong correlation was observed between the number of Ara h 2 antigens displayed on the surface of VLP Peanut particles and the vaccine's immunogenicity and protective capacity. Conclusions: Our findings demonstrate that prokaryotic RNA encapsulated within VLP Peanut, including antigen density of Ara h 2 on viral particles, are key contributors to the immunogenicity and protective capacity of the vaccine. Thus, antigenicity and RNA content are two critical quality attributes that need to be determined at the stage of manufacturing, providing robust information regarding the immunogenicity and protective capacity of VLP Peanut in the mouse which has translational relevance to the human setting. [ABSTRACT FROM AUTHOR]

Traits in Upper Palaeolithic art which are also seen in the work of talented artists with autism, including most obviously an exceptional realism, remain to be explained. However any association between the famously evocative animal depictions created in the European Upper Palaeolithic and what is commonly seen as a ‘disorder’ has always been contentious. Debate over these similarities has been heated, with explanations ranging from famous works of Upper Palaeolithic art having been created by individuals with autism spectrum conditions, to being influenced by such individuals, to being a product of the use of psychotropic drugs. Here we argue that ‘autistic traits’ in art, such as extreme realism, have been created by individuals with a cognitive extreme of local processing bias, or detail focus. The significance of local processing bias, which is found both as a feature of autism spectrum conditions and in artists with exceptional talent at realistic depiction who aren’t autistic, has implications for our understanding of Upper Palaeolithic society in general, as well as of the roles played by individuals with autism spectrum conditions.

Autism spectrum conditions are widely characterized as a cognitive difference which affects social understanding and behaviour. However, evidence increasingly suggests that the condition also affects engagement with material aspects of the environment. Here we review research into how autism affects engagement with the material world. We argue that, whilst there are important distinctions, autism affects perception of the social and material world in similar ways. Furthermore, a subtly different engagement with the material world can bring certain advantages and social contributions. We discuss implications for future research and for inclusion. [ABSTRACT FROM AUTHOR]

This paper presents contemporary archaeology as a novel framework for investigating environmental pollution, specifically marine pollution, which comprises a global "toxic assemblage" of an estimated 5.25 trillion plastic artefacts. The ideas behind this approach were developed in 2018 during a multidisciplinary "Science to Solutions" workshop held in Galápagos (Ecuador), led by the Galápagos National Park and Galapagos Conservation Trust and hosted by the Galapagos Science Center and the Charles Darwin Research Station. These ideas informed two studies which began separately but became increasingly aligned within a contemporary archaeology framework, in effect tackling the same problem from two very different perspectives: the first involving surface mapping, designed to inform an understanding of how plastic items enter the environment, including the marine environment, in the first place; and the second comprising utilization-focused evaluation, designed to better understand people's behaviours and aspirations. Both of the studies centred on a specific and ubiquitous type of item or artefact: the disposable plastic bag. We conclude that the two studies together demonstrate that, through giving primacy to material culture, contemporary archaeology can (1) serve as a cross-disciplinary framework for tackling environmental pollution, and (2) provide a basis for shaping practice and informing policy. [ABSTRACT FROM AUTHOR]