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Author's abstract: In this work, nitroxide 2,2,6,6-tetramethylpiperdine 1-oxyl (Tempo), human serum albumin (HSA), CuSO4 and phosphate buffered saline (PBS) were used to synthesis the hybrid nanoflowers. Scanning electron microscopy (SEM), electron paramagnetic resonance (EPR), and Fourier-transform infrared spectroscopy (FTIR) were used for characterization of the nanoflowers. The SEM spectra illustrated the morphologies of the hybrid nanoflowers. The FTIR spectra corroborated the presence of PNA in the hybrid nanoflowers, while the EPR spectra confirmed the intermolecular interaction of nitroxides bound to the human serum albumin incorporated into the nanoflowers. The catalase mimetic activity and corresponding kinetic parameters of nanoflowers were determined using the H2O2 depletion and O2 evolution assays. The superoxide dismutase mimetic activity of the nanoflowers was determined by the xanthine oxidase/cytochrome c method. And the reusability of the hybrid nanoflowers was assessed via the H2O2 depletion assay and the xanthine oxidase/cytochrome c method. Kinetic analysis revealed that the reactions of the incorporated nanoflowers with H2O2 obey Michaelis-Menton kinetics and the nanoflowers possess a higher affinity for H2O2 than nature catalase. the hybrid nanoflowers with superior stability and desired ligand grafting sites will find widespread utilization in the medical sciences.

Examining the process of knowledge exchange and uptake (KEU) involved collaborating with a current decision-maker to determine what knowledge would be examined to increase the understanding why evidence is accepted or rejected as evidence for decision-making. The knowledge selected were the organizational values of a Regional Health Authority from a managerial perspective. The uptake was how the values informed decision-making. The secondary attributes were identified as the conditions and characteristics that enable or challenge using values. As decision-makers prefer usable tools as a research deliverable, a Continuous Improvement Model and Logic Model were populated with the results of a mixed method inquiry. The actionable recommendations are intended to counter challenges and encourage best practices, and provide evaluation indicators. This dissertation will add to the growing literature on how to influence the uptake of evidence through collaboration and effective knowledge transfer plans that aid in bridging the know-do gap.