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Your search keyword '"Scielzo, Cristina"' showing total 249 results
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249 results on '"Scielzo, Cristina"'

1. Contributors

Author

Alaimo, Gianluca, primary, Auricchio, Ferdinando, additional, Barbugian, Federica, additional, Bloise, Nora, additional, Bonatti, Amedeo Franco, additional, Cadamuro, Francesca, additional, Cakal, Selgin D., additional, Conti, Michele, additional, De Maria, Carmelo, additional, Farè, Silvia, additional, Fassina, Lorenzo, additional, Fortunato, Gabriele Maria, additional, Kajtez, Janko, additional, Lind, Johan U., additional, Luo, Zeyu, additional, Marconi, Stefania, additional, Marino, Michele, additional, Mauri, Valeria, additional, Montagna, Giulia, additional, Mu, Xuan, additional, Negrello, Erika, additional, Pasqualini, Francesco S., additional, Pinos, Riccardo, additional, Pless, Christian J., additional, Radeke, Carmen, additional, Ribezzi, Davide, additional, Russo, Laura, additional, Santesarti, Gianluca, additional, Sbrana, Francesca V., additional, Scielzo, Cristina, additional, Scocozza, Franca, additional, Sottile, Virginie, additional, Visai, Livia, additional, Vozzi, Giovanni, additional, and Zhang, Yu Shrike, additional

Published
2022
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4. Functional Differences between IgM and IgD Signaling in Chronic Lymphocytic Leukemia

Author

Ten Hacken, Elisa, Sivina, Mariela, Kim, Ekaterina, O'Brien, Susan, Wierda, William G, Ferrajoli, Alessandra, Estrov, Zeev, Keating, Michael J, Oellerich, Thomas, Scielzo, Cristina, Ghia, Paolo, Caligaris-Cappio, Federico, and Burger, Jan A

Subjects
Biochemistry and Cell Biology, Medical Physiology, Biomedical and Clinical Sciences, Biological Sciences, Rare Diseases, Cancer, Lymphatic Research, Hematology, Lymphoma, Clinical Research, Adaptor Proteins, Signal Transducing, B-Lymphocytes, Blood Proteins, Cell Survival, Cells, Cultured, Cellular Microenvironment, Chemokine CCL3, Chemokine CCL4, Gene Expression Regulation, Humans, Immunoglobulin D, Immunoglobulin M, Intracellular Signaling Peptides and Proteins, Leukemia, Lymphocytic, Chronic, B-Cell, Lymph Nodes, Protein-Tyrosine Kinases, Proto-Oncogene Proteins c-bcl-6, Receptors, Antigen, B-Cell, Signal Transduction, Immunology, Biochemistry and cell biology
Abstract

BCR signaling is a central pathogenetic pathway in chronic lymphocytic leukemia (CLL). Most CLL cells express BCRs of IgM and IgD isotypes, but the contribution of these isotypes to functional responses remains incompletely defined. We therefore investigated differences between IgM and IgD signaling in freshly isolated peripheral blood CLL cells and in CLL cells cultured with nurselike cells, a model that mimics the lymph node microenvironment. IgM signaling induced prolonged activation of ERK kinases and promoted CLL cell survival, CCL3 and CCL4 chemokine secretion, and downregulation of BCL6, the transcriptional repressor of CCL3 In contrast, IgD signaling induced activation of the cytoskeletal protein HS1, along with F-actin polymerization, which resulted in rapid receptor internalization and failure to support downstream responses, including CLL cell survival and chemokine secretion. IgM and IgD receptor downmodulation, HS1 and ERK activation, chemokine secretion, and BCL6 downregulation were also observed when CLL cells were cocultured with nurselike cells. The Bruton's tyrosine kinase inhibitor ibrutinib effectively inhibited both IgM and IgD isotype signaling. In conclusion, through a variety of functional readouts, we demonstrate very distinct outcomes of IgM and IgD isotype activation in CLL cells, providing novel insight into the regulation of BCR signaling in CLL.

Published
2016

5. T-cell defects in patients with ARPC1B germline mutations account for combined immunodeficiency

Author

Brigida, Immacolata, Zoccolillo, Matteo, Cicalese, Maria Pia, Pfajfer, Laurène, Barzaghi, Federica, Scala, Serena, Oleaga-Quintas, Carmen, Álvarez-Álvarez, Jesus A., Sereni, Lucia, Giannelli, Stefania, Sartirana, Claudia, Dionisio, Francesca, Pavesi, Luca, Benavides-Nieto, Marta, Basso-Ricci, Luca, Capasso, Paola, Mazzi, Benedetta, Rosain, Jeremie, Marcus, Nufar, Lee, Yu Nee, Somech, Raz, Degano, Massimo, Raiola, Giuseppe, Caorsi, Roberta, Picco, Paolo, Moncada Velez, Marcela, Khourieh, Joelle, Arias, Andrés Augusto, Bousfiha, Aziz, Issekutz, Thomas, Issekutz, Andrew, Boisson, Bertrand, Dobbs, Kerry, Villa, Anna, Lombardo, Angelo, Neven, Benedicte, Moshous, Despina, Casanova, Jean-Laurent, Franco, José Luis, Notarangelo, Luigi D., Scielzo, Cristina, Volpi, Stefano, Dupré, Loïc, Bustamante, Jacinta, Gattorno, Marco, and Aiuti, Alessandro

Published
2018
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8. HIF-1α regulates the interaction of chronic lymphocytic leukemia cells with the tumor microenvironment

Author

Valsecchi, Roberta, Coltella, Nadia, Belloni, Daniela, Ponente, Manfredi, ten Hacken, Elisa, Scielzo, Cristina, Scarfò, Lydia, Bertilaccio, Maria Teresa Sabrina, Brambilla, Paola, Lenti, Elisa, Martinelli Boneschi, Filippo, Brendolan, Andrea, Ferrero, Elisabetta, Ferrarini, Marina, Ghia, Paolo, Tonon, Giovanni, Ponzoni, Maurilio, Caligaris-Cappio, Federico, and Bernardi, Rosa

Published
2016
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9. Targeting Macrophages Sensitizes Chronic Lymphocytic Leukemia to Apoptosis and Inhibits Disease Progression

Author

Galletti, Giovanni, Scielzo, Cristina, Barbaglio, Federica, Rodriguez, Tania Véliz, Riba, Michela, Lazarevic, Dejan, Cittaro, Davide, Simonetti, Giorgia, Ranghetti, Pamela, Scarfò, Lydia, Ponzoni, Maurilio, Rocchi, Martina, Corti, Angelo, Anselmo, Achille, van Rooijen, Nico, Klein, Christian, Ries, Carola H., Ghia, Paolo, De Palma, Michele, Caligaris-Cappio, Federico, and Bertilaccio, Maria Teresa Sabrina

Published
2016
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19. The Nanomechanical Properties of CLL Cells Are Linked to the Actin Cytoskeleton and Are a Potential Target of BTK Inhibitors.

Author

Sampietro, Marta, Cassina, Valeria, Salerno, Domenico, Barbaglio, Federica, Buglione, Enrico, Marrano, Claudia Adriana, Campanile, Riccardo, Scarfò, Lydia, Biedenweg, Doreen, Fregin, Bob, Zamai, Moreno, Díaz Torres, Alfonsa, Labrador Cantarero, Veronica, Ghia, Paolo, Otto, Oliver, Mantegazza, Francesco, Caiolfa, Valeria R., and Scielzo, Cristina

Published
2023
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29. Stereotyped patterns of somatic hypermutation in subsets of patients with chronic lymphocytic leukemia: implications for the role of antigen selection in leukemogenesis

Author

Murray, Fiona, Darzentas, Nikos, Hadzidimitriou, Anastasia, Tobin, Gerard, Boudjogra, Myriam, Scielzo, Cristina, Laoutaris, Nikolaos, Karlsson, Karin, Baran-Marzsak, Fanny, Tsaftaris, Athanasios, Moreno, Carol, Anagnostopoulos, Achilles, Caligaris-Cappio, Federico, Vaur, Dominique, Ouzounis, Christos, Belessi, Chrysoula, Ghia, Paolo, Davi, Fred, Rosenquist, Richard, and Stamatopoulos, Kostas

Published
2008
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30. 3D co-culture model of chronic lymphocytic leukemia bone marrow microenvironment predicts patient-specific response to mobilizing agents

Author

Barbaglio, Federica, Belloni, Daniela, Scarfò, Lydia, Sbrana, Francesca Vittoria, Ponzoni, Maurilio, Bongiovanni, Lucia, Pavesi, Luca, Zambroni, Desiree, Stamatopoulos, Kostas, Caiolfa, Valeria R, Ferrero, Elisabetta, Ghia, Paolo, Scielzo, Cristina, Barbaglio, Federica, Belloni, Daniela, Scarfò, Lydia, Sbrana, Francesca Vittoria, Ponzoni, Maurilio, Bongiovanni, Lucia, Pavesi, Luca, Zambroni, Desiree, Stamatopoulos, Kosta, Caiolfa, Valeria R, Ferrero, Elisabetta, Ghia, Paolo, and Scielzo, Cristina

Subjects
Kinase inhibitor, 3D models, Chronic Lymphocytic Leukemia, cytoskeleton, microenvironment
Abstract

Chronic Lymphocytic Leukemia (CLL) cells disseminate into supportive tissue microenvironments. To investigate the mechanisms involved in leukemic cell tissue retention we developed a 3D bone marrow (BM) microenvironment that recreates CLL - BM-stromal cells interactions inside a scaffold within a bioreactor. Our system allows the parallel analysis of CLL cells retained inside the scaffold and those released in the presence/absence of pharmacological agents, mimicking tissue and circulating cell compartments, respectively. CLL cells can be retained within the scaffold only in the presence of microenvironmental elements, which through direct contact down-regulate the expression of HS1 cytoskeletal protein in CLL cells. Consist with this, the expression of HS1 was lower in CLL cells obtained from patients' BM versus CLL cells circulating in the PB. Moreover, we demonstrate that CLL cells with inactive-HS1, impaired cytoskeletal activity and a more aggressive phenotype are more likely retained within the scaffold despite the presence of Ibrutinib, whose mobilizing effect is mainly exerted on those with active-HS1, ensuing dynamic cytoskeletal activity. This differential effect would not otherwise be assessable in a traditional 2D system and may underlie a distinctive resistance of single CLL clones. Notably, CLL cells mobilized in the peripheral blood of patients during Ibrutinib therapy exhibited activated HS1, underscoring that our model reliably mirrors the in vivo situation. The 3D model described herein is suitable to reproduce and identify critical CLL-BM interactions, opening the way to pathophysiological studies and the evaluation of novel targeted therapies in an individualized manner.

Published
2020

34. 3D Bioprinting Allows the Establishment of Long-Term 3D Culture Model for Chronic Lymphocytic Leukemia Cells

Author

Sbrana, Francesca Vittoria, primary, Pinos, Riccardo, additional, Barbaglio, Federica, additional, Ribezzi, Davide, additional, Scagnoli, Fiorella, additional, Scarfò, Lydia, additional, Redwan, Itedale Namro, additional, Martinez, Hector, additional, Farè, Silvia, additional, Ghia, Paolo, additional, and Scielzo, Cristina, additional

Published
2021
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43. B lymphocytes contribute to stromal reaction in pancreatic ductal adenocarcinoma

Author

Minici, Claudia, primary, Rigamonti, Elena, additional, Lanzillotta, Marco, additional, Monno, Antonella, additional, Rovati, Lucrezia, additional, Maehara, Takashi, additional, Kaneko, Naoki, additional, Deshpande, Vikram, additional, Protti, Maria Pia, additional, De Monte, Lucia, additional, Scielzo, Cristina, additional, Crippa, Stefano, additional, Arcidiacono, Paolo Giorgio, additional, Dugnani, Erica, additional, Piemonti, Lorenzo, additional, Falconi, Massimo, additional, Pillai, Shiv, additional, Manfredi, Angelo A., additional, and Della-Torre, Emanuel, additional

Published
2020
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44. A retinoic acid-dependent stroma-leukemia crosstalk promotes chronic lymphocytic leukemia progression

Author

Farinello, Diego, primary, Wozińska, Monika, additional, Lenti, Elisa, additional, Genovese, Luca, additional, Bianchessi, Silvia, additional, Migliori, Edoardo, additional, Sacchetti, Nicolò, additional, di Lillo, Alessia, additional, Bertilaccio, Maria Teresa Sabrina, additional, de Lalla, Claudia, additional, Valsecchi, Roberta, additional, Gleave, Sabrina Bascones, additional, Lligé, David, additional, Scielzo, Cristina, additional, Mauri, Laura, additional, Ciampa, Maria Grazia, additional, Scarfò, Lydia, additional, Bernardi, Rosa, additional, Lazarevic, Dejan, additional, Gonzalez-Farre, Blanca, additional, Bongiovanni, Lucia, additional, Campo, Elias, additional, Cerutti, Andrea, additional, Ponzoni, Maurilio, additional, Pattini, Linda, additional, Caligaris-Cappio, Federico, additional, Ghia, Paolo, additional, and Brendolan, Andrea, additional

Published
2018
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45. Synthetic high-density lipoproteins as targeted monotherapy for chronic lymphocytic leukemia

Author

McMahon, Kaylin M., primary, Scielzo, Cristina, additional, Angeloni, Nicholas L., additional, Deiss-Yehiely, Elad, additional, Scarfo, Lydia, additional, Ranghetti, Pamela, additional, Ma, Shuo, additional, Kaplan, Jason, additional, Barbaglio, Federica, additional, Gordon, Leo I., additional, Giles, Francis J., additional, Thaxton, C. Shad, additional, and Ghia, Paolo, additional

Published
2017
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46. Inhibition of chronic lymphocytic leukemia progression by full-length chromogranin A and its N-terminal fragment in mouse models

Author

Bianco, Mimma, primary, Gasparri, Anna, additional, Generoso, Luca, additional, Assi, Emma, additional, Colombo, Barbara, additional, Scarfò, Lydia, additional, Bertilaccio, Maria T.S., additional, Scielzo, Cristina, additional, Ranghetti, Pamela, additional, Dondossola, Eleonora, additional, Ponzoni, Maurilio, additional, Caligaris-Cappio, Federico, additional, Ghia, Paolo, additional, and Corti, Angelo, additional

Published
2016
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47. CARATTERIZZAZIONE DEI MECCANISMI FISIOPATOLOGICI ALLA BASE DELLA REGOLAZIONE DEI LINFOCITI B: IMPLICAZIONI NELLA LEUCEMIA LINFATICA CRONICA E POTENZIALI FATTORI PROGNOSTICI

Author

SCIELZO, CRISTINA

Abstract

In Chronic Lymphocytic Leukemia (CLL) the relationships between cell proliferation and accumulation within lymphoid organs are unclear, as are the rules that control CLL cell migration and re-circulation. We previous demonstrated that Hematopoietic cell specific Lyn substrate 1 (HS1) is involved in the natural history of CLL (Scielzo et al JCI 2005). Furthermore, we have shown that HS1 interacts with distinct cytoskeleton adapters and is likely involved in cytoskeleton reorganization (Muzio et al, Leukemia 2007). Given the role played by the cytoskeleton in controlling cellular shape, mobility and homing, we hypothesized that HS1 could be potentially relevant in the regulation of CLL cells infiltration into lymphoid tissues and re-circulation between peripheral blood and tissues. To study HS1 function in vitro, we silenced its expression in a CLL cell line (MEC1), and we tested its capacity to migrate in an in vitro and in vivo assay. We also analysed B cells from HS1 KO mice (Fukuda et al, EMBO J. 1995) and we showed that in both cellular systems HS1-deficient B cells are severely impaired in their spontaneous migration capacity. A decrease in F-actin polymerization and an increased aggregation ability was also evident in cells lacking HS1. We then observed that HS1 is central for the initiation and maintenance of B-Cell Receptor (BCR) signalling events. Indeed in the absence of HS1, cells failed to form actin-myosin complexes thereby leading to an instability of the cell signalling complex, in particular of the signalosome complex proximal to the BCR. Lyn, VAV, HIP-55, SHP-1 and RAC1/2 were directly affected by the absence of HS1 while Syk, PLC-?2 and Blnk appear to be unaffected. To test in vivo HS1 function, we injected MEC1 cells silenced for HS1 subcutaneously in RAG2-/-?c-/- mice and observed that cells lacking HS1 spread and localized preferentially in the BM and in the lymph nodes as compared to control cells. To further investigate its role played in the onset and progression of CLL, we crossed HS1 KO mice with E�-TCL1 transgenic animals (Bichi R et al, PNAS 2002). The double HS1 KO/TCL-1 interestingly show a preferential accumulation of monoclonal CD19+CD5+ cells, beside all other lymphoid organs, in the bone marrow where they are usually observed at low frequencies in the E�-TCL1 mouse. These findings suggest that HS1 has a crucial role in controlling cell migration and tissue invasion by leukemic B cells, likely through its involvement in cytoskeleton organization. This points at HS1 as a target for development of novel cancer treatments, aiming at interfering with the lymphoid tissue infiltration and invasion which is characteristic of the disease.

Published
2012
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48. IgM and IgD Receptors Differentially Contribute to CLL Survival and Chemokine Secretion: Implications for CLL Biology and Treatment

Author

ten Hacken, Elisa, primary, Sivina, Mariela, additional, Ping, Li, additional, O'Brien, Susan, additional, Wierda, William G., additional, Ferrajoli, Alessandra, additional, Estrov, Zeev, additional, Keating, Michael J., additional, Scielzo, Cristina, additional, Ghia, Paolo, additional, Caligaris-Cappio, Federico, additional, and Burger, Jan A., additional

Published
2015
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