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Additional file 1 : Supplementary Figure 1. Multi-Dimensional Scaling (MDS) plots show that samples cluster significantly differently due to their origin (human donor and mock community). Moreover, timepoint 1 (e.g.,T30-1 samples) and timepoint 2 samples (e.g.,T30-2) cluster in proximity. (A) Clustering is performed only with samples prepared using ≥100 pg DNA input (lower limit for V3-V4). (B) All dilution samples were plotted.

Additional file 2 : Supplementary Table 1. Generalized UniFrac dissimilarity matrix for ZIEL2 samples in comparison to the ideal composition at the genus level. Supplementary Table 2. Phyla-level classification of dilution series for sample T1. In “other” the following phyla are combined: Cyanobacteria, Desulfobacterota, Fusobacteriota, Verrucomicrobiota, and unknown bacteria. Supplementary Table 3. Phyla-level classification of dilution series for sample T30. In “other” the following phyla are combined: Cyanobacteria, Desulfobacterota, Fusobacteriota, Verrucomicrobiota, and unknown bacteria.