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Your search keyword '"Schroer, Martin A."' showing total 238 results
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238 results on '"Schroer, Martin A."'

10. Selection, biophysical and structural analysis of synthetic nanobodies that effectively neutralize SARS-CoV-2

Author

Custódio, Tânia F., Das, Hrishikesh, Sheward, Daniel J., Hanke, Leo, Pazicky, Samuel, Pieprzyk, Joanna, Sorgenfrei, Michèle, Schroer, Martin A., Gruzinov, Andrey Yu., Jeffries, Cy M., Graewert, Melissa A., Svergun, Dmitri I., Dobrev, Nikolay, Remans, Kim, Seeger, Markus A., McInerney, Gerald M., Murrell, Ben, Hällberg, B. Martin, and Löw, Christian

Published
2020
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17. Hydration in aqueous NaCl

Author

Sahle, Christoph J., Gallerande, Emmanuelle de Clermont, Niskanen, Johannes, Longo, Alessandro, Elbers, Mirko, Schroer, Martin A., Sternemann, Christian, Jahn, Sandro, Sahle, Christoph J., Gallerande, Emmanuelle de Clermont, Niskanen, Johannes, Longo, Alessandro, Elbers, Mirko, Schroer, Martin A., Sternemann, Christian, and Jahn, Sandro

Abstract

Atomistic details about the hydration of ions in aqueous solutions are still debated due to the disordered and statistical nature of the hydration process. However, many processes from biology, physical chemistry to materials sciences rely on the complex interplay between solute and solvent. Oxygen K-edge X-ray excitation spectra provide a sensitive probe of the local atomic and electronic surrounding of the excited sites. We used ab initio molecular dynamics simulations together with extensive spectrum calculations to relate the features found in experimental oxygen K-edge spectra of a concentration series of aqueous NaCl with the induced structural changes upon solvation of the salt and distill the spectral fingerprints of the first hydration shells around the Na+- and Cl--ions. By this combined experimental and theoretical approach, we find the strongest spectral changes to indeed result from the first hydration shells of both ions and relate the observed shift of spectral weight from the post- to the main-edge to the origin of the post-edge as a shape resonance.

Published
2022

19. Rigid-to-Flexible Transition in a Molecular Brush in a Good Solvent at a Semidilute Concentration

Author

Kang, Jia-Jhen, primary, Sachse, Clemens, additional, Ko, Chia-Hsin, additional, Schroer, Martin A., additional, Vela, Stefano Da, additional, Molodenskiy, Dmitry, additional, Kohlbrecher, Joachim, additional, Bushuev, Nikita V., additional, Gumerov, Rustam A., additional, Potemkin, Igor I., additional, Jordan, Rainer, additional, and Papadakis, Christine M., additional

Published
2022
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20. Hydration in aqueous NaCl

Author

Sahle, Christoph Johannes, primary, De Clermont Gallerande, Emmanuelle, additional, Niskanen, Johannes, additional, Longo, Alessandro, additional, Elbers, Mirko, additional, Schroer, Martin A, additional, Sternemann, Christian, additional, and Jahn, Sandro, additional

Published
2022
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25. Anomalous small-angle X-ray scattering on biological macromolecules at the P12 beamline of EMBL-Hamburg

Author

Gruzinov, Andrey, Schroer, Martin, Blanchet, Clement, Manalastas-Cantos, Karen, Kikhney, Alexey, Hajizadeh, Nelly, Wieland, Florian, Schulz, Florian, Franke, Daniel, Jeffries, Cy, and Svergun, Dmitri

Subjects
Inorganic Chemistry, Structural Biology, General Materials Science, ddc:530, Physical and Theoretical Chemistry, Condensed Matter Physics, Biochemistry
Abstract

Acta crystallographica / A 77(a2), C290 - C290 (2021). doi:10.1107/S0108767321093922, Published by Blackwell, Oxford [u.a.]

Published
2021
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26. Dependence of the nanoscale composite morphology of Fe₃O₄ nanoparticle-infused lysozyme amyloid fibrils on timing of infusion : A combined SAXS and AFM study

Author

Schroer, Martin A., Hu, Po-Sheng, Tomasovicova, Natalia, Batkova, Marianna, Zakutanska, Katarina, Wu, Po-Yi, and Kopcansky, Peter

Subjects
Chemie
Abstract

Understanding the formation process and the spatial distribution of nanoparticle (NP) clusters on amyloid fibrils is an essential step for the development of NP-based methods to inhibit aggregation of amyloidal proteins or reverse the assembling trend of the proto-fibrillary complexes that prompts pathogenesis of neuro degeneration. For this, a detailed structural determination of the diverse hybrid assemblies that are forming is needed, which can be achieved by advanced X-ray scattering techniques. Using a combined solution small angle X-ray scattering (SAXS) and atomic force microscopy (AFM) approach, this study investigates the intrinsic trends of the interaction between lysozyme amyloid fibrils (LAFs) and Fe3O4 NPs before and after fibrillization at na-nometer resolution. AFM images reveal that the number of NP clusters interacting with the lyso-zyme fibers does not increase significantly with NP volume concentration, suggesting a saturation in NP aggregation on the fibrillary surface. The data indicate that the number of non-adsorbed Fe₃O₄ NPs is highly dependent on the timing of NP infusion within the synthesis process. SAXS data yield access to the spatial distribution, aggregation manner and density of NP clusters on the fibrillary surfaces. Employing modern data analysis approaches, the shape and internal structural morphology of the so formed nanocomposites are revealed. The combined experimental approach suggests that while Fe3O4 NPs infusion does not prevent the fibril-formation, the variation of NP concentration and size at different stages of the fibrillization process can impose a pronounced impact on the superficial and internal structural morphologies of these nanocomposites. These findings may be applicable in devising advanced therapeutic treatments for neurodegenerative diseases and designing novel bio-inorganic magnetic devices. Our results further demonstrate that modern X-ray methods give access to the structure of—and insight into the formation process of—biological–inorganic hybrid structures in solution. CA Schroer

Published
2021

27. Cotton Textile/Iron Oxide Nanozyme Composites with Peroxidase-like Activity: Preparation, Characterization, and Application

Author

Safarik, Ivo, primary, Prochazkova, Jitka, additional, Schroer, Martin A., additional, Garamus, Vasil M., additional, Kopcansky, Peter, additional, Timko, Milan, additional, Rajnak, Michal, additional, Karpets, Maksym, additional, Ivankov, Oleksandr I., additional, Avdeev, Mikhail V., additional, Petrenko, Viktor I., additional, Bulavin, Leonid, additional, and Pospiskova, Kristyna, additional

Published
2021
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30. Selection, biophysical and structural analysis of synthetic nanobodies that effectively neutralize SARS-CoV-2

Author

Custódio, Tânia F, Das, Hrishikesh; https://orcid.org/0000-0001-7495-7065, Sheward, Daniel J, Hanke, Leo; https://orcid.org/0000-0001-5514-2418, Pazicky, Samuel; https://orcid.org/0000-0002-4674-9784, Pieprzyk, Joanna, Sorgenfrei, Michèle, Schroer, Martin A; https://orcid.org/0000-0002-0747-3965, Gruzinov, Andrey Yu, Jeffries, Cy M, Graewert, Melissa A; https://orcid.org/0000-0001-7547-636X, Svergun, Dmitri I, Dobrev, Nikolay, Remans, Kim, Seeger, Markus A; https://orcid.org/0000-0003-1761-8571, McInerney, Gerald M; https://orcid.org/0000-0003-2257-7241, Murrell, Ben; https://orcid.org/0000-0002-0393-4445, Hällberg, B Martin; https://orcid.org/0000-0002-6781-0345, Löw, Christian; https://orcid.org/0000-0003-0764-7483, Custódio, Tânia F, Das, Hrishikesh; https://orcid.org/0000-0001-7495-7065, Sheward, Daniel J, Hanke, Leo; https://orcid.org/0000-0001-5514-2418, Pazicky, Samuel; https://orcid.org/0000-0002-4674-9784, Pieprzyk, Joanna, Sorgenfrei, Michèle, Schroer, Martin A; https://orcid.org/0000-0002-0747-3965, Gruzinov, Andrey Yu, Jeffries, Cy M, Graewert, Melissa A; https://orcid.org/0000-0001-7547-636X, Svergun, Dmitri I, Dobrev, Nikolay, Remans, Kim, Seeger, Markus A; https://orcid.org/0000-0003-1761-8571, McInerney, Gerald M; https://orcid.org/0000-0003-2257-7241, Murrell, Ben; https://orcid.org/0000-0002-0393-4445, Hällberg, B Martin; https://orcid.org/0000-0002-6781-0345, and Löw, Christian; https://orcid.org/0000-0003-0764-7483

Abstract

The coronavirus SARS-CoV-2 is the cause of the ongoing COVID-19 pandemic. Therapeutic neutralizing antibodies constitute a key short-to-medium term approach to tackle COVID-19. However, traditional antibody production is hampered by long development times and costly production. Here, we report the rapid isolation and characterization of nanobodies from a synthetic library, known as sybodies (Sb), that target the receptor-binding domain (RBD) of the SARS-CoV-2 spike protein. Several binders with low nanomolar affinities and efficient neutralization activity were identified of which Sb23 displayed high affinity and neutralized pseudovirus with an IC$_{50}$ of 0.6 µg/ml. A cryo-EM structure of the spike bound to Sb23 showed that Sb23 binds competitively in the ACE2 binding site. Furthermore, the cryo-EM reconstruction revealed an unusual conformation of the spike where two RBDs are in the 'up' ACE2-binding conformation. The combined approach represents an alternative, fast workflow to select binders with neutralizing activity against newly emerging viruses.

Published
2020

31. Smaller capillaries improve the small-angle X-ray scattering signal and sample consumption for biomacromolecular solutions

Author

Schroer, Martin A., Blanchet, Clement E., Gruzinov, Andrey Yu., Gräwert, Melissa A., Brennich, Martha E., Hajizadeh, Nelly R., Jeffries, Cy M., and Svergun, Dmitri I.

Subjects
0301 basic medicine, 030103 biophysics, Nuclear and High Energy Physics, Materials science, Capillary action, Macromolecular Substances, Analytical chemistry, law.invention, 03 medical and health sciences, X-Ray Diffraction, law, Scattering, Small Angle, ddc:550, Absorption (electromagnetic radiation), Instrumentation, Radiation, Small-angle X-ray scattering, Scattering, Proteins, Laminar flow, protein solution, Research Papers, Synchrotron, Volumetric flow rate, Solutions, Wavelength, 030104 developmental biology, flow through sample environment, radiation damage, small-angle X-ray scattering, Synchrotrons
Abstract

Journal of synchrotron radiation 25(4), 1113 - 1122 (2018). doi:10.1107/S1600577518007907, Radiation damage by intense X-ray beams at modern synchrotron facilities is one of the major complications for biological small-angle X-ray scattering (SAXS) investigations of macromolecules in solution. To limit the damage, samples are typically measured under a laminar flow through a cell (typically a capillary) such that fresh solution is continuously exposed to the beam during measurement. The diameter of the capillary that optimizes the scattering-to-absorption ratio at a given X-ray wavelength can be calculated a priori based on fundamental physical properties. However, these well established scattering and absorption principles do not take into account the radiation susceptibility of the sample or the often very limited amounts of precious biological material available for an experiment. Here it is shown that, for biological solution SAXS, capillaries with smaller diameters than those calculated from simple scattering/absorption criteria allow for a better utilization of the available volumes of radiation-sensitive samples. This is demonstrated by comparing two capillary diameters d$_i$ (d$_i$ = 1.7 mm, close to optimal for 10 keV; and d$_i$ = 0.9 mm, which is nominally sub-optimal) applied to study different protein solutions at various flow rates. The use of the smaller capillaries ultimately allows one to collect higher-quality SAXS data from the limited amounts of purified biological macromolecules., Published by Wiley-Blackwell, [S.l.]

Published
2018

32. Heterogeneous local order in self-assembled nanoparticle films revealed by X-ray cross-correlations

Author

Lehmkühler, Felix, Schulz, Florian, Schroer, Martin, Frenzel, Lara, Lange, Holger, and Gruebel, Gerhard

Subjects
X-ray cross-correlations, Astrophysics::High Energy Astrophysical Phenomena, gold nanoparticles, ddc:530, XCCA, lcsh:Q, lcsh:Science, Research Papers, self-assembled nanoparticle films
Abstract

IUCrJ 5(3), 354 - 360 (2018). doi:10.1107/S2052252518005407, We report on the self-assembly of gold nanoparticles coated with a soft poly(ethylene glycol) shell studied by X-ray cross-correlation analysis. Depending on the initial concentration of gold nanoparticles used, structurally heterogeneous films were formed. The films feature hot spots of dominating four- and sixfold local order with patch sizes of a few micrometres, containing 10$^4$–10$^5$ particles. The amplitude of the order parameters suggested that a minimum sample amount was necessary to form well ordered local structures. Furthermore, the increasing variation in order parameters with sample thickness demonstrated a high degree of structural heterogeneity. This wealth of information cannot be obtained by the conventional microscopy techniques that are commonly used to study nanocrystal superstructures, as illustrated by complementary scanning electron microscopy measurements., Published by IUCr, Chester

Published
2018

33. Clustering in Ferronematics - The Effect of Magnetic Collective Ordering

Author

Lackova, Veronika, primary, Schroer, Martin, additional, Honecker, Dirk, additional, Hasler, Martin, additional, Vargova, Hana, additional, Zakutanska, Katarina, additional, Behrens, Silke, additional, Kovac, Jozef, additional, Svergun, Dmitri, additional, Kopcansky, Peter, additional, and Tomasovicova, Natalia, additional

Published
2021
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35. Structure of the endocytic adaptor complex reveals the basis for efficient membrane anchoring during clathrin-mediated endocytosis

Author

Lizarrondo, Javier, primary, Klebl, David P., additional, Niebling, Stephan, additional, Abella, Marc, additional, Schroer, Martin A., additional, Mertens, Haydyn D.T., additional, Veith, Katharina, additional, Svergun, Dmitri I., additional, Skruzny, Michal, additional, Sobott, Frank, additional, Muench, Stephen, additional, and Garcia-Alai, Maria M., additional

Published
2020
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36. Investigation of pH-Responsiveness inside Lipid Nanoparticles for Parenteral mRNA Application Using Small-Angle X-ray Scattering

Author

Uebbing, Lukas, primary, Ziller, Antje, additional, Siewert, Christian, additional, Schroer, Martin A., additional, Blanchet, Clement E., additional, Svergun, Dmitri I., additional, Ramishetti, Srinivas, additional, Peer, Dan, additional, Sahin, Ugur, additional, Haas, Heinrich, additional, and Langguth, Peter, additional

Published
2020
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37. Slowing down of dynamics and orientational order preceding crystallization in hard-sphere systems

Author

Lehmkühler, Felix, primary, Hankiewicz, Birgit, additional, Schroer, Martin A., additional, Müller, Leonard, additional, Ruta, Beatrice, additional, Sheyfer, Dina, additional, Sprung, Michael, additional, Tono, Kensuke, additional, Katayama, Tetsuo, additional, Yabashi, Makina, additional, Ishikawa, Tetsuya, additional, Gutt, Christian, additional, and Grübel, Gerhard, additional

Published
2020
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38. Polysarcosine-Functionalized Lipid Nanoparticles for Therapeutic mRNA Delivery

Author

Nogueira, Sara S., primary, Schlegel, Anne, additional, Maxeiner, Konrad, additional, Weber, Benjamin, additional, Barz, Matthias, additional, Schroer, Martin A., additional, Blanchet, Clement E., additional, Svergun, Dmitri I., additional, Ramishetti, Srinivas, additional, Peer, Dan, additional, Langguth, Peter, additional, Sahin, Ugur, additional, and Haas, Heinrich, additional

Published
2020
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39. Hybrid Biopolymer and Lipid Nanoparticles with Improved Transfection Efficacy for mRNA

Author

Siewert, Christian D., primary, Haas, Heinrich, additional, Cornet, Vera, additional, Nogueira, Sara S., additional, Nawroth, Thomas, additional, Uebbing, Lukas, additional, Ziller, Antje, additional, Al-Gousous, Jozef, additional, Radulescu, Aurel, additional, Schroer, Martin A., additional, Blanchet, Clement E., additional, Svergun, Dmitri I., additional, Radsak, Markus P., additional, Sahin, Ugur, additional, and Langguth, Peter, additional

Published
2020
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41. Selection, biophysical and structural analysis of synthetic nanobodies that effectively neutralize SARS-CoV-2

Author

Custódio, Tânia F., primary, Das, Hrishikesh, additional, Sheward, Daniel J, additional, Hanke, Leo, additional, Pazicky, Samuel, additional, Pieprzyk, Joanna, additional, Sorgenfrei, Michèle, additional, Schroer, Martin, additional, Gruzinov, Andrey, additional, Jeffries, Cy, additional, Graewert, Melissa, additional, Svergun, Dmitri, additional, Dobrev, Nikolay, additional, Remans, Kim, additional, Seeger, Markus A., additional, McInerney, Gerald M, additional, Murrell, Ben, additional, Hällberg, B. Martin, additional, and Löw, Christian, additional

Published
2020
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42. Structural Kinetics of MsbA Investigated by Stopped-Flow Time-Resolved Small-Angle X-Ray Scattering

Author

Josts, Inokentijs, primary, Gao, Yunyun, additional, Monteiro, Diana C.F., additional, Niebling, Stephan, additional, Nitsche, Julius, additional, Veith, Katharina, additional, Gräwert, Tobias W., additional, Blanchet, Clement E., additional, Schroer, Martin A., additional, Huse, Nils, additional, Pearson, Arwen R., additional, Svergun, Dmitri I., additional, and Tidow, Henning, additional

Published
2020
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45. Dynamics of soft nanoparticle suspensions at hard X-ray FEL sources below the radiation damage threshold

Author

Lehmkühler, Felix, Ripado Valerio, Joana, Sheyfer, Dina, Roseker, Wojciech, Schroer, Martin, Fischer, Birgit, Tono, Kensuke, Yabashi, Makina, Ishikawa, Tetsuya, and Gruebel, Gerhard

Subjects
Condensed Matter::Soft Condensed Matter, FEL, PNIPAM nanoparticles, soft-matter materials, Astrophysics::High Energy Astrophysical Phenomena, radiation-damage threshold, ddc:530, lcsh:Q, XPCS, lcsh:Science, Research Papers, SACLA
Abstract

IUCrJ 5(6), 801 - 807 (2018). doi:10.1107/S2052252518013696, The application of X-ray photon correlation spectroscopy (XPCS) at free-electron laser (FEL) facilities enables, for the first time, the study of dynamics on a (sub-)nanometre scale in an unreached time range between femtoseconds and seconds. For soft-matter materials, radiation damage is a major limitation when going beyond single-shot applications. Here, an XPCS study is presented at a hard X-ray FEL on radiation-sensitive polymeric poly(N-isopropylacrylamide) (PNIPAM) nanoparticles. The dynamics of aqueous suspensions of densely packed silica-PNIPAM core-shell particles and a PNIPAM nanogel below the radiation-damage threshold are determined. The XPCS data indicate non-diffusive behaviour, suggesting ballistic and stress-dominated heterogeneous particle motions. These results demonstrate the feasibility of XPCS experiments on radiation-sensitive soft-matter materials at FEL sources and pave the way for future applications at MHz repetition rates as well as ultrafast modes using split-pulse devices., Published by Wiley, Chester

Published
2018
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