1. Development and validation of a novel suspended particulate matter sampling device for analysis of particle-bound microbial communities.
- Author
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Shatara FJ, Yokota K, Peschman J, Kothari AJ, Schoville J, Hou L, Withington Iv RP, Beale CF, Pelusi M, Boldon KM, Withington J, Withington Iii RP, Nicklay H, Twiss MR, Paradis CJ, and Majumder EL
- Subjects
- Rivers microbiology, Rivers chemistry, Particle Size, Lakes microbiology, Lakes chemistry, Groundwater microbiology, Groundwater chemistry, Microbiota, Water Microbiology, Bacteria isolation & purification, Bacteria classification, Bacteria genetics, Particulate Matter analysis, Environmental Monitoring instrumentation, Environmental Monitoring methods
- Abstract
Biotic and abiotic materials attachment to suspended particulate matter in aquatic systems can increase their toxicity and health impacts and has led to an increased need for consistent sampling across various compartments. Sedimentation traps and continuous flow centrifuges are the traditional tools for sampling suspended particulate matter, while manta trawls have been widely used for surface water sampling of suspended or floating microplastics. Limitations, however, exist in the cost of sampling and infrastructure needed to deploy such devices. Here we report on the construction and usage of a novel suspended particulate matter sampling device, the microParticle Obtaining Trap (mPOT). Quality control testing of the mPOT showed suspended particle recovery rates of >90% for particles 100 µm and larger, while field sampling of groundwater, lake and river water shows consistent, size-fractionated recovery of particulate matter. The mPOT is well suited to sample systems not easily accessible by boat or for particles not commonly recovered by common suspended particulate matter sampling and for collection of particles smaller than 300 µm in size.
- Published
- 2025
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