Search

Your search keyword '"Schmidlin, P. R."' showing total 310 results
Start Over Author "Schmidlin, P. R."
310 results on '"Schmidlin, P. R."'

9. Limosilactobacillus reuteri supernatant attenuates inflammatory responses of human gingival fibroblasts to LPS but not to elevated glucose levels.

Author

Janson, T. M., Ramenzoni, L. L., Hatz, C. R., Schlagenhauf, U., Attin, T., and Schmidlin, P. R.

Subjects
THERAPEUTIC use of probiotics, BLOOD sugar analysis, IN vitro studies, RESEARCH funding, TREATMENT effectiveness, DESCRIPTIVE statistics, FIBROBLASTS, GENE expression, LIPOPOLYSACCHARIDES, GINGIVAL hyperplasia, INFLAMMATION, CELL survival, CYTOKINES, GRAM-positive bacteria, INTERLEUKINS, DIETARY supplements
Abstract

Aim: We investigated the in vitro effect of Limosilactobacillus reuteri DSM 17938 supernatant on the inflammatory response of human gingival fibroblasts (HGF) challenged by lipopolysaccharide (LPS) or elevated glucose levels. Methods: HGF were exposed to LPS (1 μg/mL), glucose (5, 12 mM or 25 mM), and dilutions of supernatant prepared from L. reuteri DSM 17938 (0.5 × 107, 1.0 × 107, 2.5 × 107, and 5.0 × 107 CFU/mL). After 24 h cell viability and levels of cytokines (IL‐1β, IL‐6 and IL‐8) and TLR‐2 were determined. Results: None of the tested L. reuteri (DSM 17938) supernatant concentrations reduced the viability of HGF. Supernatant concentrations (2.5 × 107 and 5 × 107 CFU/mL) significantly (p <.05) decreased the production of IL‐1β, IL‐6, IL‐8, and TLR‐2 in the presence of LPS. In contrast, inflammatory markers were not reduced by L. reuteri supernatant in the presence of glucose. Glucose concentrations of 12 mM and 24 mM still lead to an elevated production of the investigated biochemical mediators. Conclusion: While L. reuteri (DSM 17938) supernatant attenuates the inflammatory response of HGF to LPS in a dose‐dependent manner, elevated glucose levels suppress this action. These in vitro results support the overall anti‐inflammatory efficacy of L. reuteri supplementation in plaque‐associated periodontal inflammations. [ABSTRACT FROM AUTHOR]

Published
2024
Full Text
View/download PDF

18. Bacterial contamination of air and surfaces during dental procedures—An experimental pilot study using Staphylococcus aureus

Author

Franz, Jessica, Scheier, Thomas C., Aerni, Maja, Gubler, Andrea, Schreiber, Peter W., Brugger, Silvio D., and Schmidlin, Patrick R.

Abstract

AbstractObjective:The oral cavity contains numerous microorganisms, including antimicrobial-resistant bacteria. These microorganisms can be transmitted via respiratory particles from patients to healthcare providers and vice versa during dental care. We evaluated the spread of Staphylococcus aureusduring standardized dental procedures using different scaling devices and rinsing solutions.Methods:During systematic therapy for dental biofilm removal (guided biofilm therapy), using an airflow or ultrasound device to a model simulation head. Staphylococcus aureussuspension was injected into the mouth of the model to mimic saliva. Different suction devices (conventional saliva ejector or a prototype) and rising solutions (water or chlorhexidine) were used. To assess contamination with S. aureus, an air-sampling device was placed near the oral cavity and samples of surface areas were collected.Results:S. aureuswas only detected by air sampling when the conventional saliva ejector with airflow was used. No growth was observed during treatments with the ultrasonic piezo instrument or the prototype suction device. Notably, a rinsing solution of chlorhexidine digluconate decreased the bacterial load compared to water. Surface contamination was rarely detected (1 of 120 samples).Conclusions:Although our findings indicate potential airborne bacterial transmission during routine prophylactic procedures, specific treatment options during biofilm removal appear to reduce air contamination. These options include ultrasonic piezo devices or the prototype suction device. The use of chlorhexidine reduced the CFU counts of S. aureusdetected by air sampling. Surface contamination during dental procedures was a rare occurrence.

Published
2024
Full Text
View/download PDF

23. In vitro evaluation of different protective techniques to reduce titanium particle contamination during implantoplasty

Author

Platt, A, Liu, C C, Gubler, A, Naenni, Nadja; https://orcid.org/0000-0002-6689-2684, Thoma, D, Schmidlin, P R, Platt, A, Liu, C C, Gubler, A, Naenni, Nadja; https://orcid.org/0000-0002-6689-2684, Thoma, D, and Schmidlin, P R

Abstract

OBJECTIVES Our aim is to study titanium remains in a bone model during standardized implantoplasty under different isolation and protective modalities. MATERIAL AND METHODS Forty implants were placed in artificial spongy bone blocks mimicking a horizontal bone loss and implant neck protrusion of 5 mm. Samples were randomly divided into four groups (n = 10), which were treated as follows: rubber dam (A), a dental adhesive paste (B), bone wax (C), and an unprotected positive control (D). Implantoplasty was performed using carbide and diamond burs under strict water cooling and standardized suction. After removal of the respective isolation materials, the bone blocks were thoroughly rinsed with tap water for 3 min and titanium chips were collected using a filter integrated in the model. The filter paper was removed and dissolved in 37% hydrochloric acid for 2 h at 120 °C and the titanium remnants were quantified using atomic absorption spectrometry. RESULTS None of the test groups were able to completely prevent titanium particle contamination. Rubber dam (691 ± 249 µg) and bone wax (516 ± 157 µg) were found to be significantly more protective than the positive control (2313 ± 747 µg) (p < 0.001) with respect to the amount of titanium particles that remained in the bone model after implantoplasty. The adhesive paste group (1863.5 ± 538 µg) was not significantly different from the positive control (p = 0.19). CONCLUSIONS Despite some limitations of the present study, titanium particles resulting from a standardized implantoplasty can be assumed to be significantly reduced when the tissues/bone were protected with rubber dam and bone wax, or a combination, depending on individual accessibility. CLINICAL RELEVANCE Tissue protective measures to reduce or avoid particle contamination during implantoplasty is possible and should be considered and further clinically assessed to avoid iatrogenic inflammatory reactions.

Published
2023

34. Bone defect development in experimental canine peri-implantitis models: a systematic review

Author

Solderer, A; https://orcid.org/0000-0002-8161-4738, de Boer, M, Wiedemeier, D B, Solderer, M, Liu, C C, Schmidlin, P R, Solderer, A; https://orcid.org/0000-0002-8161-4738, de Boer, M, Wiedemeier, D B, Solderer, M, Liu, C C, and Schmidlin, P R

Abstract

PURPOSE To provide a systematic overview of preclinical research regarding bone defect formation around different implant surfaces after ligature-induced peri-implantitis models in dogs. Two focused questions were formulated: 'How much bone loss can be expected after a certain time of ligature induced peri-implantitis?' and 'Do different implant types, dog breeds and study protocols differ in their extent of bone loss?' MATERIALS AND METHODS A systematic literature search was conducted on four databases (MEDLINE, Web of Science, EMBASE and Scopus). Observations, which consisted of bone defects measured directly after ligature removal in canine models, were included and analysed. Two approaches were used to analyse the relatively heterogeneous studies that fulfilled the inclusion criteria. First, separate simple linear regressions were calculated for each study and implant surface, for which observations were available across multiple time points. Second, a linear mixed model was specified for the observations at 12 weeks after ligature initiation, and assessing the potential influencing factors on defect depth was explored using lasso regularisation. RESULTS Thirty-six studies with a total of 1082 implants were included after. Bone loss was determined at different time points, either with clinical measurements radiographically or histologically. Different implant groups [e.g. turned, sand-blasted-acid-etched (SLA), titanium-plasma-sprayed (TPS) and other rough surfaces] were assessed and described in the studies. A mean incremental defect depth increase of 0.08 mm (SD: -0.01-0.28 mm) per week was observed. After 12 weeks, the defect depths ranged between 0.7 and 5 mm. Based on the current data set, implant surface could not be statistically identified as an essential factor in defect depth after 12 weeks of ligature-induced peri-implantitis. CONCLUSION Expectable defect depth after a specific time of ligature-induced peri-implantitis can vary robustly. It is current

Published
2022

49. Novel Grafting Materials for Bone Regeneration

Author

Duncan, W, Coates, D E, Li, K C, Schmidlin, P R, Liu, J, Cotton, G, Abdelmonein, D, Jude, A, Sheftel, Y, Lander, L, Elahi, A, Gee, C, Waddel, N, University of Zurich, Bartold, P Mark, and Ali, Tara B Taiyeb

Subjects
10066 Clinic of Conservative and Preventive Dentistry, 610 Medicine & health
Published
2020

Catalog

Books, media, physical & digital resources
See catalog results