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2. Role of Gut Microbota in Etiopathogenesis of Chronic Intestinal Psueudo Obstruction

Author

Radocchia, Giulia, primary, Neroni, Bruna, additional, Marazzato, Massimiliano, additional, Capuzzo, Elena, additional, Zuccari, Simone, additional, Pantanella, Fabrizio, additional, Zenzeri, Letizia, additional, Evangelisti, Melania, additional, Vassallo, Francesca, additional, Parisi, Pasquale, additional, Nardo, Giovanni Di, additional, and Schippa, Serena, additional

Published
2024
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5. Women Skin Microbiota Modifications during Pregnancy

Author

Radocchia, Giulia, primary, Brunetti, Francesca, additional, Marazzato, Massimiliano, additional, Totino, Valentina, additional, Neroni, Bruna, additional, Bonfiglio, Giulia, additional, Conte, Antonietta Lucia, additional, Pantanella, Fabrizio, additional, Ciolli, Paola, additional, and Schippa, Serena, additional

Published
2024
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8. Poliprotect vs Omeprazole in the Relief of Heartburn, Epigastric Pain, and Burning in Patients Without Erosive Esophagitis and Gastroduodenal Lesions: A Randomized, Controlled Trial

Author

Corazziari, Enrico Stefano, primary, Gasbarrini, Antonio, additional, D'Alba, Lucia, additional, D'Ovidio, Valeria, additional, Riggio, Oliviero, additional, Passaretti, Sandro, additional, Annibale, Bruno, additional, Cicala, Michele, additional, Repici, Alessandro, additional, Bassotti, Gabrio, additional, Ciacci, Carolina, additional, Di Sabatino, Antonio, additional, Neri, Matteo, additional, Bragazzi, Maria Consiglia, additional, Ribichini, Emanuela, additional, Radocchia, Giulia, additional, Iovino, Paola, additional, Marazzato, Massimiliano, additional, Schippa, Serena, additional, and Badiali, Danilo, additional

Published
2023
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9. Poliprotect vs Omeprazole in the relief of heartburn, epigastric pain and burning in patients without erosive esophagitis and gastro-duodenal lesions: A Randomized, Controlled Trial

Author

Corazziari, Enrico Stefano, Gasbarrini, Antonio, D'Alba, Lucia, D'Ovidio, Valeria, Riggio, Oliviero, Passaretti, Sandro, Annibale, Bruno, Cicala, Michele, Repici, Alessandro, Bassotti, Gabrio, Ciacci, Carolina, Di Sabatino, Antonio, Neri, Matteo, Bragazzi, Maria Consiglia, Ribichini, Emanuela, Radocchia, Giulia, Iovino, Paola, Marazzato, Massimiliano, Schippa, Serena, Badiali, Danilo, Gasbarrini, Antonio (ORCID:0000-0002-7278-4823), Corazziari, Enrico Stefano, Gasbarrini, Antonio, D'Alba, Lucia, D'Ovidio, Valeria, Riggio, Oliviero, Passaretti, Sandro, Annibale, Bruno, Cicala, Michele, Repici, Alessandro, Bassotti, Gabrio, Ciacci, Carolina, Di Sabatino, Antonio, Neri, Matteo, Bragazzi, Maria Consiglia, Ribichini, Emanuela, Radocchia, Giulia, Iovino, Paola, Marazzato, Massimiliano, Schippa, Serena, Badiali, Danilo, and Gasbarrini, Antonio (ORCID:0000-0002-7278-4823)

Abstract

Background: In the treatment of upper GI endoscopy-negative patients with heartburn and epigastric pain or burning, antacids, anti-reflux agents, and mucosal protective agents (MPA) are widely used, alone or as add-on treatment to increase response to proton pump inhibitors, which are not indicated in infancy and pregnancy, and account for significant cost expenditure. Aims methods: In this randomized, controlled, double-blind, double-dummy, multicenter trial assessing the efficacy and safety of MPA Poliprotect (neoBianacid®) versus Omeprazole in the relief of heartburn and epigastric pain/burning, 275 endoscopy-negative outpatients were given a 4-week treatment with Omeprazole (20 mg q.d.) or Poliprotect (5 times a day for the initial 2 weeks, and on-demand thereafter), followed by an open-label 4-week treatment period with Poliprotect on demand. Gut microbiota change was assessed. Results: A 2-week treatment with Poliprotect proved non-inferior to Omeprazole for symptom relief (between-group difference in the change in VAS symptom score: [mean, 95% CI] -5.4, -9.9 to -0.1; -6.2, -10.8 to -1.6; ITT and PP populations, respectively).Poliprotect's benefit remained unaltered after shifting to on-demand intake, with no gut microbiota variation. The initial benefit of Omeprazole was maintained against significantly higher use of rescue medicine sachets (mean, 95% CI: Poliprotect 3.9, 2.8-5.0; Omeprazole 8.2, 4.8-11.6) and associated with an increased abundance of oral cavity genera in the intestinal microbiota. No relevant adverse events were reported in either treatment arm. Conclusions: Poliprotect proved non-inferior to standard-dose Omeprazole in symptomatic patients with heartburn/epigastric burning without erosive esophagitis and gastro-duodenal lesions. Gut microbiota was not affected by Poliprotect treatment. The study is registered in Clinicaltrial.gov (NCT03238534) and the EudraCT Database (2015-005216-15).

Published
2023

13. Gut Microbiota Structure and Metabolites, Before and After Treatment in Early Rheumatoid Arthritis Patients: A Pilot Study

Author

Marazzato, Massimiliano, primary, Iannuccelli, Cristina, additional, Guzzo, Maria Paola, additional, Nencioni, Lucia, additional, Lucchino, Bruno, additional, Radocchia, Giulia, additional, Gioia, Chiara, additional, Bonfiglio, Giulia, additional, Neroni, Bruna, additional, Guerrieri, Francesca, additional, Pantanella, Fabrizio, additional, Garzoli, Stefania, additional, Vomero, Marta, additional, Barbati, Cristiana, additional, Di Franco, Manuela, additional, and Schippa, Serena, additional

Published
2022
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14. Solvent Casting and UV Photocuring for Easy and Safe Fabrication of Nanocomposite Film Dressings

Author

Di Muzio, Laura, primary, Simonetti, Prisca, additional, Carriero, Vito Cosimo, additional, Brandelli, Chiara, additional, Trilli, Jordan, additional, Sergi, Claudia, additional, Tirillò, Jacopo, additional, Cairone, Francesco, additional, Cesa, Stefania, additional, Radocchia, Giulia, additional, Schippa, Serena, additional, Petralito, Stefania, additional, Paolicelli, Patrizia, additional, and Casadei, Maria Antonietta, additional

Published
2022
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15. Mo1318 THE MUCOSAL PROTECTIVE AGENT POLIPROTECT IS AN EFFECTIVE TREATMENT TO MANAGE PPI DE-PRESCRIBING. IN NON-EROSIVE HEARTBURN AND EPIGASTRIC PAIN SYNDROME PATIENTS. RCT POST HOC ANALYSIS DATA

Author

Corazziari, Enrico, Gasbarrini, Antonio, D'Alba, Lucia, D'Ovidio, Valeria, Riggio, Oliviero, Passaretti, Sandro, Annibale, Bruno, Cicala, Michele, Repici, Alessandro, Bassotti, Gabrio, Ciacci, Carolina, Sabatino, Antonio Di, Neri, Matteo, Bragazzi, Maria Consiglia, Ribichini, Emanuela, Radocchia, Giulia, Iovino, Paola, Marazzato, Massimiliano, Schippa, Serena, and Badiali, Danilo

Published
2024
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16. Su1635 PATIENTS WITH NONEROSIVE HEARTBURN AND EPIGATRIC PAIN SYNDROME (EPS) ARE NOT CLINICALLY DISTINGUISHABLE AND BENEFIT OF THE SAME TREATMENT. RCT POST HOC ANALYSIS DATA

Author

Corazziari, Enrico, Gasbarrini, Antonio, D'Alba, Lucia, D'Ovidio, Valeria, Riggio, Oliviero, Passaretti, Sandro, Annibale, Bruno, Cicala, Michele, Repici, Alessandro, Bassotti, Gabrio, Ciacci, Carolina, Sabatino, Antonio Di, Neri, Matteo, Bragazzi, Maria Consiglia, Ribichini, Emanuela, Radocchia, Giulia, Iovino, Paola, Marazzato, Massimiliano, Schippa, Serena, and Badiali, Danilo

Published
2024
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19. Chronic Intestinal Pseudo-Obstruction: Is There a Connection with Gut Microbiota?

Author

Radocchia, Giulia, primary, Neroni, Bruna, additional, Marazzato, Massimiliano, additional, Capuzzo, Elena, additional, Zuccari, Simone, additional, Pantanella, Fabrizio, additional, Zenzeri, Letizia, additional, Evangelisti, Melania, additional, Vassallo, Francesca, additional, Parisi, Pasquale, additional, Di Nardo, Giovanni, additional, and Schippa, Serena, additional

Published
2021
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20. Lactoferrin downregulates pro-inflammatory cytokines upexpressed in intestinal epithelial cells infected with invasive or noninvasive Escherichia coli strains (1)

Author

Berlutti, Francesca, Schippa, Serena, Morea, Clara, Sarli, Serena, Perfetto, Brunella, Donnarumma, Giovanna, and Valenti, Piera

Subjects
Cytokines -- Research -- Genetic aspects, Gene expression -- Research -- Genetic aspects, Escherichia coli infections -- Research -- Genetic aspects, Epithelial cells -- Research, Biological sciences, Research, Genetic aspects
Abstract

Abstract: Intestinal epithelial cells are able to differentially interact with commensal or pathogenic microorganisms, triggering a physiological or destructive inflammation, respectively. To mimic commensal-enteroinvasive bacteria-host cell interaction, we infected Caco-2 [...]

Published
2006

21. Ala160 and His116 residues are involved in activity and specificity of apyrase, an ATP-hydrolysing enzyme produced by enteroinvasive Escherichia coli

Author

Sarli, Serena, Nicoletti, Mauro, Schippa, Serena, Del Chierico, Federica, Santapaola, Daniela, Valenti, Piera, and Berlutti, Francesca

Subjects
Escherichia coli -- Genetic aspects, Adenosine triphosphate -- Research, Gene mutations -- Research, Mutagenesis -- Research, Biological sciences
Abstract

The virulence plasmid-carried apy (phoN2) gene of Shigella and related enteroinvasive Escherichia coli (EIEC) encodes apyrase, an ATP-diphosphohydrolase belonging to class A of the non-specific acid phosphatases (A-NSAPs). Apyrase and A-NSAPs share three domains of conserved amino acids (domains D1-D3) containing residues forming the putative active site of apyrase. In spite of their similarity, apyrase and A-NSAPs show different substrate specificity, apyrase being able to hydrolyse nucleotide tri- and diphosphates, but not monophosphates, as well as p-nitrophenyl phosphate (pNPP), while A-NSAPs are also active towards monophosphates and pNPP. In this paper, to get further insights into the structure--function relationship of apyrase, a random and site-directed mutagenesis of the apy gene of EIEC strain HN280 was conducted. Results indicate that amino acids located within the D2 and D3 conserved domains (Ser157 and Arg192, respectively) as well as residues located in the N-terminal (Ser97) and C-terminal (Glu233) domains are required for enzyme activity. Surprisingly, Ala160, located near the D2 domain and considered to be important for enzyme specificity, is required for enzyme activity, as its substitution with Thr led to the inactivation of enzyme activity. Furthermore, residue His116 is involved in apyrase specificity, since the H116L apyrase mutant shows substrate specificity resembling that of A-NSAPs.

Published
2005

24. Effect of Urban Wastewater Discharge on the Abundance of Antibiotic Resistance Genes and Antibiotic-Resistant Escherichia coli in Two Italian Rivers

Author

Pantanella, Fabrizio, primary, Lekunberri, Itziar, additional, Gagliardi, Antonella, additional, Venuto, Giuseppe, additional, Sànchez-Melsió, Alexandre, additional, Fabiani, Massimo, additional, Balcázar, José Luis, additional, Schippa, Serena, additional, De Giusti, Maria, additional, Borrego, Carles, additional, and Solimini, Angelo, additional

Published
2020
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26. Structural Variations of Vaginal and Endometrial Microbiota: Hints on Female Infertility

Author

Riganelli, Lucia, primary, Iebba, Valerio, additional, Piccioni, Mariagrazia, additional, Illuminati, Isabella, additional, Bonfiglio, Giulia, additional, Neroni, Bruna, additional, Calvo, Ludovica, additional, Gagliardi, Antonella, additional, Levrero, Massimo, additional, Merlino, Lucia, additional, Mariani, Marianna, additional, Capri, Oriana, additional, Pietrangeli, Daniela, additional, Schippa, Serena, additional, and Guerrieri, Francesca, additional

Published
2020
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27. Nasal Microbiota in RSV Bronchiolitis

Author

Schippa, Serena, primary, Frassanito, Antonella, additional, Marazzato, Massimiliano, additional, Nenna, Raffaella, additional, Petrarca, Laura, additional, Neroni, Bruna, additional, Bonfiglio, Giulia, additional, Guerrieri, Francesca, additional, Frasca, Federica, additional, Oliveto, Giuseppe, additional, Pierangeli, Alessandra, additional, and Midulla, Fabio, additional

Published
2020
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29. SARS-CoV-2 and microbiota

Author

Schippa, Serena and Pantanella, Fabrizio

Subjects
sars-cov2, covid19, probiotics, microbiota
Published
2020

30. Efficacy of acoustic waves in preventing Streptococcus mutans adhesion on dental unit water line

Author

Pantanella, Fabrizio, Schippa, Serena, Solimini, Angelo, Rosa, Luigi, Bettucci, Andrea, and Berlutti, Francesca

Subjects
dental unit water lines (duwls), acoustic waves, microbial disinfections, Pilot Projects, Dental Equipment, Disinfection, Streptococcus mutans, Sound, Biofilms, Equipment Contamination, Humans, Water Microbiology, Disinfectants
Abstract

The quality and health safety of water used for refrigeration and flushing of the handpieces, water-syringes and other components of dental units is of considerable importance. Water crosses these devices by a system of intersected small plastic tubes (about 2 mm of diameter), named dental unit water lines (DUWLs). DUWLs may be heavily colonized by many bacterial species in a planktonic phase, adherent or in biofilm lifestyle, resulting in a potential risk of infection, not only for all professionals who routinely use these devices, but also for occasional-patients, especially immunocompromised patients. Contamination of DUWLs can be prevented or reduced with the use of disinfectants, but the eradication of microorganisms, especially which those are adherent or living in biofilm lifestyle on the inner surfaces of DUWLs is challenging and often, the normal methods of water disinfection are not effective. Moreover, disinfectants routinely used to disinfect DUWLs may alter the bond strength of the dentine bonding agent used for restorative practice in dentistry.To identify an innovative and alternative strategy, able to prevent bacterial adhesion to DUWL surfaces through a physical approach, which is more effective in overcoming the problem of DUWL contamination and the risk of infection compared to the standard methods already in use. In this pilot study we tested a member of the oral streptococci family, that is not a component of the biofilm detected on the walls of DUWL, but is frequently detected in water samples from DUWL, due to human fluid retraction during dental therapy. Namely, the pathogenic bacterial species Streptococcus mutans.We employ elastic acoustic waves at high-energy in preventing S. mutans adhesion to the inner walls of an experimental water circuit reproducing a DUWLs. To stress the capability of acoustic waves to interfere with bacterial adhesion also in extreme conditions, a high S. mutans contamination load was adopted.We observe a significant decrease of adherent bacteria exposed to acoustic waves treatment respect to control.This study demonstrates the effectiveness of acoustic waves in counteracting the adhesion of S. mutans to the inner walls of an experimental water circuit reproducing a DUWL, opening up new prospects for future practical applications. The interesting results, so far obtained, require an in-depth analysis of the methods regarding both the various bacterial species involved and the infective charges to be used.

Published
2019

31. Cloning and characterization of the NapA acid phosphatase phosphotransferase of Morganella morganii: identification of a new family of bacterial acid-phosphatase-encoding genes

Author

Thaller, Maria Cristina, Lombardi, Giovanna, Berlutti, Francesca, Schippa, Serena, and Rossolini, Gian Maria

Subjects
Bacteria -- Research, Acid phosphatase -- Analysis, Nucleotide sequence -- Analysis, Biological sciences
Abstract

The gene coding for the Morganella morganii phosphate-irrepressible acid phosphatase (NapA) is an acid phosphatase that has four polypeptide subunits of 27 kDa each. The enzyme has a transphosphorylating potential from organic phosphoric acid esters to nucleoside and other free hydroxyl group-containing compounds. Inorganic phosphate, EDTA, Ca2+ and nucleosides prevent enzyme activity while Co2+, Hg2+ and Zn2+ and Zn2+ promote the same. The NapA enzyme does not exhibit sequence similarity with any sequenced bacterial phosphatase but is similar to the proteins encoded by sequenced regions in the open reading frames of Escherichia coli and Proteus mirabilis genomes. It is proposed that NapA is a member of the new class B-family bacterial acid phosphatases.

Published
1995

32. Characterization and sequence of PhoC, the principal phosphate-irrepressible acid phosphatase of Morganella morganii

Author

Thaller, Maria Cristina, Berlutti, Francesca, Schippa, Serena, Lombardi, Giovanna, and Rossolini, Gian Maria

Subjects
Enterobacteriaceae -- Genetic aspects, Acid phosphatase -- Analysis, Amino acid sequence -- Analysis, Biological sciences
Abstract

PhoC, the phosphate-irrepressible acid phosphatase of Morganella morganii, resembles the HPAP phenotype. The presence of the PhoC activity inhibits the induction of alkaline phosphatase. The amino acid sequence and regulation of PhoC are different from other members of the gene family of enterobacteria, resulting in a different phylogeny.

Published
1994

34. Growth Control of Adherent-Invasive Escherichia coli (AIEC) by the Predator Bacteria Bdellovibrio bacteriovorus: A New Therapeutic Approach for Crohn’s Disease Patients

Author

Bonfiglio, Giulia, primary, Neroni, Bruna, additional, Radocchia, Giulia, additional, Pompilio, Arianna, additional, Mura, Francesco, additional, Trancassini, Maria, additional, Di Bonaventura, Giovanni, additional, Pantanella, Fabrizio, additional, and Schippa, Serena, additional

Published
2019
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35. Nasal microbiota in RSV microbiota

Author

Persia, Sabrina, primary, Frassanito, Antonella, additional, Nenna, Raffaella, additional, Petrarca, Laura, additional, Di Mattia, Greta, additional, Merola, Antonella, additional, Iebba, Valerio, additional, Schippa, Serena, additional, Scagnolari, Carolina, additional, Pierangeli, Alessandra, additional, and Midulla, Fabio, additional

Published
2019
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36. A distinctive 'microbial signature' in celiac pediatric patients

Author

Longhi Catia, Checchi Monica, Totino Valentina, Di Nardo Giovanni, Barbato Maria, Iebba Valerio, Schippa Serena, Maiella Giulia, Cucchiara Salvatore, and Conte Maria

Subjects
Microbiology, QR1-502
Abstract

Abstract Background Celiac Disease (CD) is an autoimmune disorder of the small intestine in which dietary gluten ingestion leads to a chronic enteropathy. Recently, scientific evidence suggested a potential role of gut microbiota in CD. To have a snapshot of dominant duodenal microbiota we analyzed the mucosa-associated microbiota of 20 children with CD, before and after a gluten-free diet (GFD) regimen, and of 10 controls. Total DNA was extracted from duodenal biopsies and amplification products of 16S ribosomal DNA were compared by temporal temperature gradient gel electrophoresis (TTGE). TTGE profiles were analyzed by statistical multivariate analysis. Results The average number of bands in TTGE profiles was significantly higher (P < 0.0001) in active (n.b. 16.7 ± 0.7) and inactive states (n.b. 13.2 ± 0.8) than in controls (n.b. 3.7 ± 1.3). Mean interindividual similarity index was 54.9% ± 14.9% for active disease, 55.6% ± 15.7% for remission state and 21.8% ± 30.16% for controls. Similarity index between celiac children before and after GFD treatment was 63.9% ± 15.8%. Differences in microbiota biodiversity were among active and remission state (P = 0.000224) and amid active CD and controls (P < 0.001). Bacteroides vulgatus and Escherichia coli were detected more often in CD patients than in controls (P < 0.0001). Conclusions Overall, the results highlighted a peculiar microbial TTGE profile and a significant higher biodiversity in CD pediatric patients' duodenal mucosa. The possible pathophysiological role of these microbial differences needs further characterization.

Published
2010
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38. Rebuilding the Gut Microbiota Ecosystem

Author

Gagliardi, Antonella, primary, Totino, Valentina, additional, Cacciotti, Fatima, additional, Iebba, Valerio, additional, Neroni, Bruna, additional, Bonfiglio, Giulia, additional, Trancassini, Maria, additional, Passariello, Claudio, additional, Pantanella, Fabrizio, additional, and Schippa, Serena, additional

Published
2018
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39. 1014 - Microbiota Composition, Metabolic Profiles and Inflammatory Host Response after Fecal Microbiota Transplantation (FMT) for Recurrent Clostridium Difficile Infection. Does Proteobacteria Abundance Predict the Response to FMT?

Author

Schippa, Serena, primary, Bruno, Giovanni, additional, Cicerone, Clelia, additional, Badiali, Danilo, additional, Auria, Stefania, additional, Zaccari, Piera, additional, Casadio, Marco, additional, Trancassini, Maria, additional, Gagliardi, Antonella, additional, Neroni, Bruna, additional, Bonfiglio, Giulia, additional, Oliva, Alessandra, additional, D'abramo, Alessandra, additional, Iebba, Valerio, additional, Macone, Alberto, additional, Vullo, Vincenzo, additional, and Corazziari, Enrico, additional

Published
2018
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40. Postbiotic Protective Activity of Lactobacillus Rhamnosus GG-Derived Factors on Pathogen Lipopolysaccharide (LPS)-Induced Damage of Human Colonic Smooth Muscle

Author

Cicenia, Alessia, Santangelo, Floriana, Gambardella, Lucia, Iebba, Valerio, Scirocco, Annunziata, Marignani, Michela, Chirletti, Piero, Pallotta, Lucia, Carabotti, Marilia, Corazziari, Enrico Stefano, Schippa, Serena, Severi, Carola, Cicenia, Alessia, Santangelo, Floriana, Gambardella, Lucia, Iebba, Valerio, Scirocco, Annunziata, Marignani, Michela, Chirletti, Piero, Pallotta, Lucia, Carabotti, Marilia, Corazziari, Enrico Stefano, Schippa, Serena, and Severi, Carola

Published
2015

43. Molecular Detection of the Predatory Bacterium Bdellovibrio bacteriovorus from Dental Biofilms.

Author

Passariello, Claudio, Schippa, Serena, Di Nardo, Dario, Polimeni, Antonella, and Testarelli, Luca

Subjects
BIOFILMS, BACTERIA, ORAL diseases, PROKARYOTES
Abstract

Introduction: The oral microbiome is a complex community whose composition results from multiple interactions among different microorganisms and with the host. Predatory prokaryotes are recognized as important balancing factors in different ecosystems. Among them Bdellovibrio bacteriovorus received special attention for its strong predatory behaviour against many human pathogens in the absence of any toxic or pathogenic effect, so that it was proposed as a live probiotic/antibiotic agent. This work aimed to evaluate if B. bacteriovorus is detected from samples of human oral and dental biofilm. Materials and Methods: Samples of oral and dental biofilm were obtained from 20 adults of both sexes and processed for extraction of metagenomic DNA, to be used as templates for B. bacteriovorus-specific PCR reactions. Specificity of amplification products was confirmed by sequencing. Results: All 20 dental biofilm samples and 12 of 20 (60%) oral biofilm samples were resulted PCR positive. The sequences of 17 of 32 PCR products (53.1%) showed 100% identity with the reference sequence; the sequences of 11 of 32 PCR products (34.4%) showed ≥99% identity, while the remaining 4 products (12.5%) showed identities ranging between <99% and ≥97%. Conclusions: This is the first survey specifically reporting the presence of B. bacteriovorus in the human oral cavity and suggests that bacterial predation is a relevant balancing factor for the oral microbiota. Demonstration that B. bacteriovorus is able to colonize the oral cavity gives strength to proposals of its use as a probiotic/antibiotic in the prevention/treatment of selected oral diseases. [ABSTRACT FROM AUTHOR]

Published
2019
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44. Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) Allelic Variants Relate to Shifts in Faecal Microbiota of Cystic Fibrosis Patients

Author

Schippa, Serena, Iebba, Valerio, Santangelo, Floriana, Antonella, Gagliardi, DE BIASE, Riccardo Valerio, Antonella, Stamato, Serenella, Bertasi, Lucarelli, Marco, Conte, Maria Pia, Quattrucci, Serena, Neeraj, Vij, Schippa, Serena, Iebba, Valerio, Santangelo, Floriana, Antonella, Gagliardi, DE BIASE, Riccardo Valerio, Antonella, Stamato, Serenella, Bertasi, Lucarelli, Marco, Conte, Maria Pia, Quattrucci, Serena, and Neeraj, Vij

Subjects
Male, Cystic Fibrosis, Genotyping Techniques, Applied Microbiology, Cystic Fibrosis Transmembrane Conductance Regulator, micorbiota, Severity of Illness Index, Cystic fibrosis, Feces, fluids and secretions, Autosomal Recessive, RNA, Ribosomal, 16S, Respiratory system, Child, Electrophoresis, Agar Gel, Escherichia Coli, Gastrointestinal tract, Multidisciplinary, Ecology, biology, Discriminant Analysis, cftr, Middle Aged, Cystic fibrosis transmembrane conductance regulator, Bacterial Pathogens, Phenotype, medicine.anatomical_structure, Medical Microbiology, Child, Preschool, Medicine, Female, Pediatric Gastroenterology, Research Article, Adult, congenital, hereditary, and neonatal diseases and abnormalities, Adolescent, Science, Ileum, Gastroenterology and Hepatology, Microbiology, Microbial Ecology, Young Adult, Species Specificity, Meconium, Genetic Mutation, Genetics, medicine, Humans, Least-Squares Analysis, Allele, Biology, cystic fibrosi, Alleles, Demography, Clinical Genetics, Human Genetics, medicine.disease, Mucus, cystic fibrosis, Mutation, Genetics of Disease, Immunology, biology.protein, Metagenome
Abstract

IntroductionIn this study we investigated the effects of the Cystic Fibrosis Transmembrane conductance Regulator (CFTR) gene variants on the composition of faecal microbiota, in patients affected by Cystic Fibrosis (CF). CFTR mutations (F508del is the most common) lead to a decreased secretion of chloride/water, and to mucus sticky secretions, in pancreas, respiratory and gastrointestinal tracts. Intestinal manifestations are underestimated in CF, leading to ileum meconium at birth, or small bowel bacterial overgrowth in adult age.MethodsThirty-six CF patients, fasting and under no-antibiotic treatment, were CFTR genotyped on both alleles. Faecal samples were subjected to molecular microbial profiling through Temporal Temperature Gradient Electrophoresis and species-specific PCR. Ecological parameters and multivariate algorithms were employed to find out if CFTR variants could be related to the microbiota structure.ResultsPatients were classified by two different criteria: 1) presence/absence of F508del mutation; 2) disease severity in heterozygous and homozygous F508del patients. We found that homozygous-F508del and severe CF patients exhibited an enhanced dysbiotic faecal microbiota composition, even within the CF cohort itself, with higher biodiversity and evenness. We also found, by species-specific PCR, that potentially harmful species (Escherichia coli and Eubacterium biforme) were abundant in homozygous-F508del and severe CF patients, while beneficial species (Faecalibacterium prausnitzii, Bifidobacterium spp., and Eubacterium limosum) were reduced.ConclusionsThis is the first report that establishes a link among CFTR variants and shifts in faecal microbiota, opening the way to studies that perceive CF as a 'systemic disease', linking the lung and the gut in a joined axis.

Published
2013

45. Characterization of mucosa-associated and fecal microbiota of children with autism spectre disorder

Author

IEBBA, VALERIO, SCHIPPA, Serena, VALITUTTI, FRANCESCO, SANTANGELO, FLORIANA, S. Gatti, I. Celletti, S. Leoni, TROVATO, CHIARA MARIA, IORFIDA, DONATELLA, P. Di Matteo, Marazzato, Massimiliano, CONTE, Maria Pia, BARBATO, Maria, Iebba, Valerio, Schippa, Serena, Valitutti, Francesco, Santangelo, Floriana, S., Gatti, I., Celletti, S., Leoni, Trovato, CHIARA MARIA, Iorfida, Donatella, P., Di Matteo, Marazzato, Massimiliano, Conte, Maria Pia, and Barbato, Maria

Published
2013

46. Cystic fibrosis transmembrane conductance regulator (CFTR) allelic variants relate to shifts in fecal microbiota of cystic fibrosis patients

Author

Iebba, Valerio, Schippa, Serena, Santangelo, Floriana, DE BIASE, Riccardo Valerio, Stamato, Antonella, Bertasi, S., Rolla, M., Lucarelli, Marco, Conte, Maria Pia, Quattrucci, Serena, Iebba, Valerio, Schippa, Serena, Santangelo, Floriana, DE BIASE, Riccardo Valerio, Stamato, Antonella, S., Bertasi, M., Rolla, Lucarelli, Marco, Conte, Maria Pia, and Quattrucci, Serena

Published
2012

47. Gut microbiota related to Giardia duodenalis, Entamoeba spp. and Blastocystis hominis infections in humans from Cote d'Ivoire

Author

Iebba, Valerio, Santangelo, Floriana, Totino, Valentina, Pantanella, Fabrizio, Monsia, Anatole, Di Cristanziano, Veronica, Di Cave, David, Schippa, Serena, Berrilli, Federica, D'Alfonso, Rossella, Iebba, Valerio, Santangelo, Floriana, Totino, Valentina, Pantanella, Fabrizio, Monsia, Anatole, Di Cristanziano, Veronica, Di Cave, David, Schippa, Serena, Berrilli, Federica, and D'Alfonso, Rossella

Abstract

Introduction: Literature data provide little information about protozoa infections and gut microbiota compositional shifts in humans. This preliminary study aimed to describe the fecal bacterial community composition of people from Cote d'Ivoire harboring Giardia duodenalis, Entamoeba spp., and Blastocystis hominis, in trying to discover possible alterations in their fecal microbiota structure related to the presence of such parasites. Methodology: Twenty fecal samples were collected from people inhabiting three different localities of Cote d'Ivoire for copromicroscopic analysis and molecular identification of G. duodenalis, Entamoeba spp., and B. hominis. Temporal temperature gradient gel electrophoresis (TTGE) was used to obtain a fingerprint of the overall bacterial community; quantitative polymerase chain reaction (qPCR) was used to define the relative abundances of selected bacterial species/group, and multivariate statistical analyses were employed to correlate all data. Results: Cluster analysis revealed a significant separation of TTGE profiles into four clusters (p < 0.0001), with a marked difference for G. duodenalis-positive samples in relation to the others (p = 5.4x10(-6)). Interestingly, qPCR data showed how G. duodenalis-positive samples were related to a dysbiotic condition that favors potentially harmful species (such as Escherichia coli), while Entamoeba spp./B. hominis-positive subjects were linked to a eubiotic condition, as shown by a significantly higher Faecalibacterium prausnitzii-Escherichia coli ratio. Conclusions: This preliminary investigation demonstrates a differential fecal microbiota structure in subjects infected with G. duodenalis or Entamoeba spp./B. hominis, paving the way for using further next-generation DNA technologies to better understand host-parasite-bacteria interactions, aimed at identifying potential indicators of microbiota changes.

Published
2016

48. Protective Role of Postbiotic Mediators Secreted by Lactobacillus rhamnosus GG Versus Lipopolysaccharide-induced Damage in Human Colonic Smooth Muscle Cells

Author

Cicenia, Alessia, primary, Santangelo, Floriana, additional, Gambardella, Lucrezia, additional, Pallotta, Lucia, additional, Iebba, Valerio, additional, Scirocco, Annunziata, additional, Marignani, Massimo, additional, Tellan, Guglielmo, additional, Carabotti, Marilia, additional, Corazziari, Enrico Stefano, additional, Schippa, Serena, additional, and Severi, Carola, additional

Published
2016
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49. Gut microbiota related to Giardia duodenalis, Entamoeba spp. and Blastocystis hominis infections in humans from Côte d’Ivoire

Author

Iebba, Valerio, primary, Santangelo, Floriana, additional, Totino, Valentina, additional, Pantanella, Fabrizio, additional, Monsia, Anatole, additional, Di Cristanziano, Veronica, additional, Di Cave, David, additional, Schippa, Serena, additional, Berrilli, Federica, additional, and D'Alfonso, Rossella, additional

Published
2016
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50. Mo1927 Mucosa-Associated Microbiota and Promoter Methylation Status of Genes Involved in Immune Response in Crohn's Disease Patients

Author

Cicerone, Clelia, primary, Totino, Valentina, additional, Iebba, Valerio, additional, Vernia, Piero, additional, Pontone, Stefano, additional, Porowska, Barbara, additional, Giovannone, Maurizio, additional, Santangelo, Floriana, additional, Gagliardi, Antonella, additional, Guerrieri, Francesca, additional, Levrero, Massimo, additional, Pantanella, Fabrizio, additional, Schippa, Serena, additional, and Corazziari, Enrico, additional

Published
2016
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