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18 results on '"Schinnerer, Meike"'

3. Density of Conjugated Antibody Determines the Extent of Fc Receptor Dependent Capture of Nanoparticles by Liver Sinusoidal Endothelial Cells

Author

Kappel, Cinja, primary, Seidl, Christine, additional, Medina-Montano, Carolina, additional, Schinnerer, Meike, additional, Alberg, Irina, additional, Leps, Christian, additional, Sohl, Julian, additional, Hartmann, Ann-Kathrin, additional, Fichter, Michael, additional, Kuske, Michael, additional, Schunke, Jenny, additional, Kuhn, Gabor, additional, Tubbe, Ingrid, additional, Paßlick, David, additional, Hobernik, Dominika, additional, Bent, Rebekka, additional, Haas, Katharina, additional, Montermann, Evelyn, additional, Walzer, Kerstin, additional, Diken, Mustafa, additional, Schmidt, Manfred, additional, Zentel, Rudolf, additional, Nuhn, Lutz, additional, Schild, Hansjörg, additional, Tenzer, Stefan, additional, Mailänder, Volker, additional, Barz, Matthias, additional, Bros, Matthias, additional, and Grabbe, Stephan, additional

Published
2021
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4. Polymeric Nanoparticles: Polymeric Nanoparticles with Neglectable Protein Corona (Small 18/2020)

Author

Alberg, Irina, primary, Kramer, Stefan, additional, Schinnerer, Meike, additional, Hu, Qizhi, additional, Seidl, Christine, additional, Leps, Christian, additional, Drude, Natascha, additional, Möckel, Diana, additional, Rijcken, Cristianne, additional, Lammers, Twan, additional, Diken, Mustafa, additional, Maskos, Michael, additional, Morsbach, Svenja, additional, Landfester, Katharina, additional, Tenzer, Stefan, additional, Barz, Matthias, additional, and Zentel, Rudolf, additional

Published
2020
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5. Polymeric Nanoparticles with Neglectable Protein Corona

Author

Alberg, Irina, primary, Kramer, Stefan, additional, Schinnerer, Meike, additional, Hu, Qizhi, additional, Seidl, Christine, additional, Leps, Christian, additional, Drude, Natascha, additional, Möckel, Diana, additional, Rijcken, Cristianne, additional, Lammers, Twan, additional, Diken, Mustafa, additional, Maskos, Michael, additional, Morsbach, Svenja, additional, Landfester, Katharina, additional, Tenzer, Stefan, additional, Barz, Matthias, additional, and Zentel, Rudolf, additional

Published
2020
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6. Targeting of Immune Cells with Trimannosylated Liposomes

Author

Wagener, Karolin, primary, Bros, Matthias, additional, Krumb, Matthias, additional, Langhanki, Jens, additional, Pektor, Stefanie, additional, Worm, Matthias, additional, Schinnerer, Meike, additional, Montermann, Evelyn, additional, Miederer, Matthias, additional, Frey, Holger, additional, Opatz, Till, additional, and Rösch, Frank, additional

Published
2020
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7. Site-specific DBCO modification of DEC205 antibody for polymer conjugation

Author

Beck, Simone, Schultze, Jennifer, Räder, Hans-Joachim, Holm, Regina, Schinnerer, Meike, Barz, Matthias, Koynov, Kaloian, and Zentel, Rudolf

Subjects
540 Chemistry and allied sciences, RAFT polymerization, pDNA polyplex, vaccination, bioorthogonal chemistry, Article, DEC205 antibody, lcsh:QD241-441, strain-promoted alkyne-azide cycloaddition (SPAAC), lcsh:Organic chemistry, 540 Chemie, cancer immune therapy, dendritic cells (DCs), targeting
Abstract

The design of multifunctional polymer-based vectors, forming pDNA vaccines, offers great potential in cancer immune therapy. The transfection of dendritic immune cells (DCs) with tumour antigen-encoding pDNA leads to an activation of the immune system to combat tumour cells. In this work, we investigated the chemical attachment of DEC205 antibodies (aDEC205) as DC-targeting structures to polyplexes of P(Lys)-b-P(HPMA). The conjugation of a synthetic block copolymer and a biomacromolecule with various functionalities (aDEC205) requires bioorthogonal techniques to avoid side reactions. Click chemistry and in particular the strain-promoted alkyne-azide cycloaddition (SPAAC) can provide the required bioorthogonality. With regard to a SPAAC of both components, we firstly synthesized two different azide-containing block copolymers, P(Lys)-b-P(HPMA)-N-3(stat) and P(Lys)-b-P(HPMA)-N-3(end), for pDNA complexation. In addition, the site-specific incorporation of ring-strained dibenzocyclooctyne (DBCO) moieties to the DEC205 antibody was achieved by an enzymatic strategy using bacterial transglutaminase (BTG). The chemical accessibility of DBCO molecules within aDEC205 as well as the accessibility of azide-functionalities on the polyplex' surface were investigated by various SPAAC experiments and characterized by fluorescence correlation spectroscopy (FCS).

Published
2018
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11. Sekundärstrukturbildung als Triebkraft für die Selbstorganisation reaktiver Polypept(o)ide: Steuerung von Größe, Form und Funktion kernvernetzter Nanostrukturen

Author

Klinker, Kristina, primary, Schäfer, Olga, additional, Huesmann, David, additional, Bauer, Tobias, additional, Capelôa, Leon, additional, Braun, Lydia, additional, Stergiou, Natascha, additional, Schinnerer, Meike, additional, Dirisala, Anjaneyulu, additional, Miyata, Kanjiro, additional, Osada, Kensuke, additional, Cabral, Horacio, additional, Kataoka, Kazunori, additional, and Barz, Matthias, additional

Published
2017
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12. Secondary-Structure-Driven Self-Assembly of Reactive Polypept(o)ides: Controlling Size, Shape, and Function of Core Cross-Linked Nanostructures

Author

Klinker, Kristina, primary, Schäfer, Olga, additional, Huesmann, David, additional, Bauer, Tobias, additional, Capelôa, Leon, additional, Braun, Lydia, additional, Stergiou, Natascha, additional, Schinnerer, Meike, additional, Dirisala, Anjaneyulu, additional, Miyata, Kanjiro, additional, Osada, Kensuke, additional, Cabral, Horacio, additional, Kataoka, Kazunori, additional, and Barz, Matthias, additional

Published
2017
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13. Drug Delivery: Dendritic Mesoporous Silica Nanoparticles for pH-Stimuli-Responsive Drug Delivery of TNF-Alpha (Adv. Healthcare Mater. 13/2017)

Author

Kienzle, Arne, primary, Kurch, Sven, additional, Schlöder, Janine, additional, Berges, Carsten, additional, Ose, Robert, additional, Schupp, Jonathan, additional, Tuettenberg, Andrea, additional, Weiss, Henning, additional, Schultze, Jennifer, additional, Winzen, Svenja, additional, Schinnerer, Meike, additional, Koynov, Kaloian, additional, Mezger, Markus, additional, Haass, Nikolas K., additional, Tremel, Wolfgang, additional, and Jonuleit, Helmut, additional

Published
2017
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14. Dendritic Mesoporous Silica Nanoparticles for pH‐Stimuli‐Responsive Drug Delivery of TNF‐Alpha

Author

Kienzle, Arne, primary, Kurch, Sven, additional, Schlöder, Janine, additional, Berges, Carsten, additional, Ose, Robert, additional, Schupp, Jonathan, additional, Tuettenberg, Andrea, additional, Weiss, Henning, additional, Schultze, Jennifer, additional, Winzen, Svenja, additional, Schinnerer, Meike, additional, Koynov, Kaloian, additional, Mezger, Markus, additional, Haass, Nikolas K., additional, Tremel, Wolfgang, additional, and Jonuleit, Helmut, additional

Published
2017
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15. Copper-catalyzed click reactions: quantification of retained copper using 64Cu-spiked Cu(I), exemplified for CuAAC reactions on liposomes.

Author

Wagener, Karolin, Renisch, Dennis, Schinnerer, Meike, Worm, Matthias, Jakob, Yvonne, Eberhardt, Klaus, and Rösch, Frank

Subjects
LIPOSOMES, RESEARCH reactors, DRUG delivery systems, QUINAZOLINONES
Abstract

The Cu(I)-catalyzed azide-alkyne cycloaddition (CuAAC) is a powerful, highly reliable and selective reaction which allows for a rapid synthesis in high yields and under mild conditions (pH, temperature). However, the cytotoxicity of copper requires its complete removal prior to an application in vivo. This is an issue especially when it comes to CuAAC reactions on macromolecular structures or drug delivery systems, as copper might be retained by these systems. Thus, a quantification of the final copper content of these systems is inevitable, which we exemplified for a CuAAC reaction on liposomes using 64Cu-spiked Cu(I). In this respect, a Cu(II) nitrate solution was irradiated at the TRIGA Mark II research reactor Mainz to obtain c.a. [64Cu]Cu(II). The irradiated solution was directly used for a CuAAC on liposomes. After purification, their copper content was calculated utilizing γ-ray spectrometry. Only 0.018% of the added 64Cu-activity was still present in the liposome containing fractions after purification. This refers to a total amount of copper of 0.17 ng. The amount of retained copper is so low, that an in vivo application of the liposomes is absolutely reasonable. Besides this particular study, the experimental methodology may be applied to study many other CuAAC reactions, used for the synthesis of radiolabeled or non-radioactive species, which are intended for human applications. [ABSTRACT FROM AUTHOR]

Published
2019
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17. Copper-catalyzed click reactions: quantification of retained copper using 64Cu-spiked Cu(I), exemplified for CuAAC reactions on liposomes

Author

Wagener, Karolin, Renisch, Dennis, Schinnerer, Meike, Worm, Matthias, Jakob, Yvonne, Eberhardt, Klaus, and Rösch, Frank

Abstract

The Cu(I)-catalyzed azide-alkyne cycloaddition (CuAAC) is a powerful, highly reliable and selective reaction which allows for a rapid synthesis in high yields and under mild conditions (pH, temperature). However, the cytotoxicity of copper requires its complete removal prior to an application in vivo. This is an issue especially when it comes to CuAAC reactions on macromolecular structures or drug delivery systems, as copper might be retained by these systems. Thus, a quantification of the final copper content of these systems is inevitable, which we exemplified for a CuAAC reaction on liposomes using 64Cu-spiked Cu(I). In this respect, a Cu(II) nitrate solution was irradiated at the TRIGA Mark II research reactor Mainz to obtain c.a.[64Cu]Cu(II). The irradiated solution was directly used for a CuAAC on liposomes. After purification, their copper content was calculated utilizing γ-ray spectrometry. Only 0.018% of the added 64Cu-activity was still present in the liposome containing fractions after purification. This refers to a total amount of copper of 0.17 ng. The amount of retained copper is so low, that an in vivoapplication of the liposomes is absolutely reasonable. Besides this particular study, the experimental methodology may be applied to study many other CuAAC reactions, used for the synthesis of radiolabeled or non-radioactive species, which are intended for human applications.

Published
2019
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18. Comparison of Linear and Hyperbranched Polyether Lipids for Liposome Shielding by 18 F-Radiolabeling and Positron Emission Tomography.

Author

Wagener K, Worm M, Pektor S, Schinnerer M, Thiermann R, Miederer M, Frey H, and Rösch F

Subjects
Animals, Fluorine Radioisotopes, Glycerol chemistry, Liposomes pharmacokinetics, Male, Mice, Mice, Inbred C57BL, Polyethylene Glycols chemistry, Polymers chemistry, Radiopharmaceuticals, Tissue Distribution, Ethers chemistry, Lipids chemistry, Liposomes chemistry, Positron-Emission Tomography methods
Abstract

Multifunctional and highly biocompatible polyether structures play a key role in shielding liposomes from degradation in the bloodstream, providing also multiple functional groups for further attachment of targeting moieties. In this work hyperbranched polyglycerol ( hbPG) bearing lipids with long alkyl chain anchor are evaluated with respect to steric stabilization of liposomes. The branched polyether lipids possess a hydrophobic bis(hexadecyl)glycerol membrane anchor for the liposomal membrane. hbPG was chosen as a multifunctional alternative to PEG, enabling the eventual linkage of multiple targeting vectors. Different hbPG lipids ( M n = 2900 and 5200 g mol -1 ) were examined. A linear bis(hexadecyl)glycerol-PEG lipid ( M n = 3000 g mol -1 ) was investigated as well, comparing hbPG and PEG with respect to shielding properties. Radiolabeling of the polymers was carried out using 1-azido-2-(2-(2-[ 18 F]fluoroethoxy)ethoxy)ethane ([ 18 F]F-TEG-N) 3 via copper-catalyzed alkyne-azide cycloaddition with excellent radiochemical yields exceeding 95%. Liposomes were prepared by the thin-film hydration method followed by repeated extrusion. Use of a custom automatic extrusion device gave access to reproducible sizes of the liposomes (hydrodynamic radius of 60-94 nm). The in vivo fate of the bis(hexadecyl)glycerol polyethers and their corresponding assembled liposome structures were evaluated via noninvasive small animal positron emission tomography (PET) imaging and biodistribution studies (1 h after injection and 4 h after injection) in mice. Whereas the main uptake of the nonliposomal polyether lipids was observed in the kidneys and in the bladder after 1 h due to rapid renal clearance, in contrast, the corresponding liposomes showed uptake in the blood pool as well as in organs with good blood supply, that is, heart and lung over the whole observation period of 4 h. The in vivo behavior of all three liposomal formulations was comparable, albeit with remarkable differences in splenic uptake. Overall, liposomes shielded by the branched polyglycerol lipids show a favorable biodistribution with greatly prolonged blood circulation times, rendering them promising novel nanovesicles for drug transport and targeting.

Published
2018
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