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3 results on '"Schilling KB"'

1. The Marine Microbial Eukaryote Transcriptome Sequencing Project (MMETSP): Illuminating the Functional Diversity of Eukaryotic Life in the Oceans through Transcriptome Sequencing

Author

Keeling, PJ, Burki, F, Wilcox, HM, Allam, B, Allen, EE, Amaral-Zettler, LA, Armbrust, EV, Archibald, JM, Bharti, AK, Bell, CJ, Beszteri, B, Bidle, KD, Cameron, CT, Campbell, L, Caron, DA, Cattolico, RA, Collier, JL, Coyne, K, Davy, SK, Deschamps, P, Dyhrman, ST, Edvardsen, B, Gates, RD, Gobler, CJ, Greenwood, SJ, Guida, SM, Jacobi, JL, Jakobsen, KS, James, ER, Jenkins, B, John, U, Johnson, MD, Juhl, AR, Kamp, A, Katz, LA, Kiene, R, Kudryavtsev, A, Leander, BS, Lin, S, Lovejoy, C, Lynn, D, Marchetti, A, McManus, G, Nedelcu, AM, Menden-Deuer, S, Miceli, C, Mock, T, Montresor, M, Moran, MA, Murray, S, Nadathur, G, Nagai, S, Ngam, PB, Palenik, B, Pawlowski, J, Petroni, G, Piganeau, G, Posewitz, MC, Rengefors, K, Romano, G, Rumpho, ME, Rynearson, T, Schilling, KB, Schroeder, DC, Simpson, AGB, Slamovits, CH, Smith, DR, Smith, GJ, Smith, SR, Sosik, HM, Stief, P, Theriot, E, Twary, SN, Umale, PE, Vaulot, D, Wawrik, B, Wheeler, GL, Wilson, WH, Xu, Y, Zingone, A, Worden, AZ, Keeling, PJ, Burki, F, Wilcox, HM, Allam, B, Allen, EE, Amaral-Zettler, LA, Armbrust, EV, Archibald, JM, Bharti, AK, Bell, CJ, Beszteri, B, Bidle, KD, Cameron, CT, Campbell, L, Caron, DA, Cattolico, RA, Collier, JL, Coyne, K, Davy, SK, Deschamps, P, Dyhrman, ST, Edvardsen, B, Gates, RD, Gobler, CJ, Greenwood, SJ, Guida, SM, Jacobi, JL, Jakobsen, KS, James, ER, Jenkins, B, John, U, Johnson, MD, Juhl, AR, Kamp, A, Katz, LA, Kiene, R, Kudryavtsev, A, Leander, BS, Lin, S, Lovejoy, C, Lynn, D, Marchetti, A, McManus, G, Nedelcu, AM, Menden-Deuer, S, Miceli, C, Mock, T, Montresor, M, Moran, MA, Murray, S, Nadathur, G, Nagai, S, Ngam, PB, Palenik, B, Pawlowski, J, Petroni, G, Piganeau, G, Posewitz, MC, Rengefors, K, Romano, G, Rumpho, ME, Rynearson, T, Schilling, KB, Schroeder, DC, Simpson, AGB, Slamovits, CH, Smith, DR, Smith, GJ, Smith, SR, Sosik, HM, Stief, P, Theriot, E, Twary, SN, Umale, PE, Vaulot, D, Wawrik, B, Wheeler, GL, Wilson, WH, Xu, Y, Zingone, A, and Worden, AZ

Published
2014

2. The influence of food matrices on aptamer selection by SELEX (systematic evolution of ligands by exponential enrichment) targeting the norovirus P-Domain.

Author

Schilling KB, DeGrasse J, and Woods JW

Subjects
Aptamers, Nucleotide chemistry, Capsid Proteins chemistry, Capsid Proteins genetics, Humans, Ligands, Norovirus isolation & purification, Norovirus metabolism, Protein Domains genetics, Recombinant Proteins biosynthesis, Recombinant Proteins chemistry, Recombinant Proteins isolation & purification, Aptamers, Nucleotide metabolism, Capsid Proteins metabolism, Food virology, SELEX Aptamer Technique
Abstract

This study investigates the enrichment of aptamers targeting the norovirus protruding domain in the presence of foods often associated with norovirus outbreaks. The goal is to explore if and how the presence of food alters in vitro selection of aptamers and target binding of the enriched oligonucleotides. Our study demonstrates that the introduction of food to SELEX (systematic evolution of ligands by exponential enrichment) is either detrimental to enrichment of oligonucleotides with target-specific binding, or facilitates enrichment of non-target-specific oligonucleotides. Moreover, a relationship between target binding of enriched oligonucleotides in presence of food and their selection condition was not observed. Our findings also suggest that a pathogen specific aptamer with application in food does not need to be selected in presence of the particular food, but may require properties beyond high affinity and selectivity to be applied for pathogen extraction and detection in undiluted food matrices., (Published by Elsevier Ltd.)

Published
2018
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3. The Marine Microbial Eukaryote Transcriptome Sequencing Project (MMETSP): illuminating the functional diversity of eukaryotic life in the oceans through transcriptome sequencing.

Author

Keeling PJ, Burki F, Wilcox HM, Allam B, Allen EE, Amaral-Zettler LA, Armbrust EV, Archibald JM, Bharti AK, Bell CJ, Beszteri B, Bidle KD, Cameron CT, Campbell L, Caron DA, Cattolico RA, Collier JL, Coyne K, Davy SK, Deschamps P, Dyhrman ST, Edvardsen B, Gates RD, Gobler CJ, Greenwood SJ, Guida SM, Jacobi JL, Jakobsen KS, James ER, Jenkins B, John U, Johnson MD, Juhl AR, Kamp A, Katz LA, Kiene R, Kudryavtsev A, Leander BS, Lin S, Lovejoy C, Lynn D, Marchetti A, McManus G, Nedelcu AM, Menden-Deuer S, Miceli C, Mock T, Montresor M, Moran MA, Murray S, Nadathur G, Nagai S, Ngam PB, Palenik B, Pawlowski J, Petroni G, Piganeau G, Posewitz MC, Rengefors K, Romano G, Rumpho ME, Rynearson T, Schilling KB, Schroeder DC, Simpson AG, Slamovits CH, Smith DR, Smith GJ, Smith SR, Sosik HM, Stief P, Theriot E, Twary SN, Umale PE, Vaulot D, Wawrik B, Wheeler GL, Wilson WH, Xu Y, Zingone A, and Worden AZ

Subjects
Databases, Factual, Molecular Sequence Data, Sequence Analysis, Biodiversity, Environmental Microbiology, Eukaryota, Oceans and Seas, Transcriptome
Abstract

Competing Interests: The authors have declared that no competing interests exist.

Published
2014
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