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1,479 results on '"Schiffelers, Raymond"'

2. Intratumoral delivery of lipid nanoparticle-formulated mRNA encoding IL-21, IL-7, and 4-1BBL induces systemic anti-tumor immunity

Author

Hamouda, Ahmed E. I., Filtjens, Jessica, Brabants, Elisabeth, Kancheva, Daliya, Debraekeleer, Ayla, Brughmans, Jan, Jacobs, Lotte, Bardet, Pauline M. R., Knetemann, Elisabeth, Lefesvre, Pierre, Allonsius, Lize, Gontsarik, Mark, Varela, Ismael, Crabbé, Marian, Clappaert, Emile J., Cappellesso, Federica, Caro, Aarushi A., Gordún Peiró, Alícia, Fredericq, Luna, Hadadi, Eva, Estapé Senti, Mariona, Schiffelers, Raymond, van Grunsven, Leo A., Aboubakar Nana, Frank, De Geest, Bruno G., Deschoemaeker, Sofie, De Koker, Stefaan, Lambolez, Florence, and Laoui, Damya

Published
2024
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11. mRNA-LNP vaccines tuned for systemic immunization induce strong antitumor immunity by engaging splenic immune cells

Author

Bevers, Sanne, Kooijmans, Sander A.A., Van de Velde, Elien, Evers, Martijn J.W., Seghers, Sofie, Gitz-Francois, Jerney J.J.M., van Kronenburg, Nicky C.H., Fens, Marcel H.A.M., Mastrobattista, Enrico, Hassler, Lucie, Sork, Helena, Lehto, Taavi, Ahmed, Kariem E., El Andaloussi, Samir, Fiedler, Katja, Breckpot, Karine, Maes, Michael, Van Hoorick, Diane, Bastogne, Thierry, Schiffelers, Raymond M., and De Koker, Stefaan

Published
2022
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15. A modular strategy for extracellular vesicle-mediated CRISPR-Cas9 delivery through aptamer-based loading and UV-activated cargo release

Author

Elsharkasy, Omnia M., primary, Hegeman, Charlotte V., additional, Lansweers, Ivana, additional, Cotugno, Olaf L., additional, de Groot, Ingmar Y., additional, de Wit, Zoe E.N.M.J., additional, Liang, Xiuming, additional, Garcia-Guerra, Antonio, additional, Moorman, Niels J.A., additional, Lefferts, Julliet, additional, de Voogt, Willemijn S., additional, Gitz-Francois, Jerney J., additional, van Wesel, Annet C.W., additional, El Andaloussi, Samir, additional, Schiffelers, Raymond M., additional, Kooijmans, Sander A.A., additional, Mastrobattista, Enrico, additional, Vader, Pieter, additional, and de Jong, Olivier G., additional

Published
2024
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20. Extracellular vesicle-mediated delivery of CRISPR/Cas9 ribonucleoprotein complex targeting proprotein convertase subtilisin-kexin type 9 (Pcsk9) in primary mouse hepatocytes

Author

Ilahibaks, Nazma F., Kluiver, Thomas A., de Jong, Olivier G., de Jager, Saskia C.A., Schiffelers, Raymond M., Vader, Pieter, Peng, Weng Chuan, Lei, Zhiyong, Sluijter, Joost P.G., Ilahibaks, Nazma F., Kluiver, Thomas A., de Jong, Olivier G., de Jager, Saskia C.A., Schiffelers, Raymond M., Vader, Pieter, Peng, Weng Chuan, Lei, Zhiyong, and Sluijter, Joost P.G.

Abstract

The loss-of-function of the proprotein convertase subtilisin–kexin type 9 (Pcsk9) gene has been associated with significant reductions in plasma serum low-density lipoprotein cholesterol (LDL-C) levels. Both CRISPR/Cas9 and CRISPR-based editor-mediated Pcsk9 inactivation have successfully lowered plasma LDL-C and PCSK9 levels in preclinical models. Despite the promising preclinical results, these studies did not report how vehicle-mediated CRISPR delivery inactivating Pcsk9 affected low-density lipoprotein receptor recycling in vitro or ex vivo. Extracellular vesicles (EVs) have shown promise as a biocompatible delivery vehicle, and CRISPR/Cas9 ribonucleoprotein (RNP) has been demonstrated to mediate safe genome editing. Therefore, we investigated EV-mediated RNP targeting of the Pcsk9 gene ex vivo in primary mouse hepatocytes. We engineered EVs with the rapamycin-interacting heterodimer FK506-binding protein (FKBP12) to contain its binding partner, the T82L mutant FKBP12-rapamycin binding (FRB) domain, fused to the Cas9 protein. By integrating the vesicular stomatitis virus glycoprotein on the EV membrane, the engineered Cas9 EVs were used for intracellular CRISPR/Cas9 RNP delivery, achieving genome editing with an efficacy of ±28.1% in Cas9 stoplight reporter cells. Administration of Cas9 EVs in mouse hepatocytes successfully inactivated the Pcsk9 gene, leading to a reduction in Pcsk9 mRNA and increased uptake of the low-density lipoprotein receptor and LDL-C. These readouts can be used in future experiments to assess the efficacy of vehicle-mediated delivery of genome editing technologies targeting Pcsk9. The ex vivo data could be a step towards reducing animal testing and serve as a precursor to future in vivo studies for EV-mediated CRISPR/Cas9 RNP delivery targeting Pcsk9.

Published
2024

22. mRNA-LNP COVID-19 Vaccine Lipids Induce Complement Activation and Production of Proinflammatory Cytokines: Mechanisms, Effects of Complement Inhibitors, and Relevance to Adverse Reactions

Author

Centraal Diagnostisch Laboratorium, Cancer, Circulatory Health, Bakos, Tamás, Mészáros, Tamás, Kozma, Gergely Tibor, Berényi, Petra, Facskó, Réka, Farkas, Henriette, Dézsi, László, Heirman, Carlo, de Koker, Stefaan, Schiffelers, Raymond, Glatter, Kathryn Anne, Radovits, Tamás, Szénási, Gábor, Szebeni, János, Centraal Diagnostisch Laboratorium, Cancer, Circulatory Health, Bakos, Tamás, Mészáros, Tamás, Kozma, Gergely Tibor, Berényi, Petra, Facskó, Réka, Farkas, Henriette, Dézsi, László, Heirman, Carlo, de Koker, Stefaan, Schiffelers, Raymond, Glatter, Kathryn Anne, Radovits, Tamás, Szénási, Gábor, and Szebeni, János

Published
2024

23. A magnetic separation method for isolating and characterizing the biomolecular corona of lipid nanoparticles

Author

CDL Nanomedicine, Centraal Diagnostisch Laboratorium, Cancer, Circulatory Health, Francia, Valentina, Zhang, Yao, Cheng, Miffy Hok Yan, Schiffelers, Raymond M, Witzigmann, Dominik, Cullis, Pieter R, CDL Nanomedicine, Centraal Diagnostisch Laboratorium, Cancer, Circulatory Health, Francia, Valentina, Zhang, Yao, Cheng, Miffy Hok Yan, Schiffelers, Raymond M, Witzigmann, Dominik, and Cullis, Pieter R

Published
2024

24. Assessment of feto-placental oxygenation and perfusion in a rat model of placental insufficiency using T2* mapping and 3D dynamic contrast-enhanced MRI

Author

CDL Nanomedicine, Centraal Diagnostisch Laboratorium, Cancer, Circulatory Health, MS Verloskunde, Child Health, Arts-assistenten Kinderen, Al Darwish, Fatimah M., Coolen, Bram F., van Kammen, Caren M., Alles, Lindy K., de Vos, Judith, Schiffelers, Raymond M., Lely, Titia A., Strijkers, Gustav J., Terstappen, Fieke, CDL Nanomedicine, Centraal Diagnostisch Laboratorium, Cancer, Circulatory Health, MS Verloskunde, Child Health, Arts-assistenten Kinderen, Al Darwish, Fatimah M., Coolen, Bram F., van Kammen, Caren M., Alles, Lindy K., de Vos, Judith, Schiffelers, Raymond M., Lely, Titia A., Strijkers, Gustav J., and Terstappen, Fieke

Published
2024

25. Biofluid specific protein coronas affect lipid nanoparticle behavior in vitro

Author

CDL Staf Research, CDL Nanomedicine, CDL extracellular vesicles, CDL Contact activatie, Centraal Diagnostisch Laboratorium, Cancer, Circulatory Health, van Straten, Demian, Sork, Helena, van de Schepop, Luuk, Frunt, Rowan, Ezzat, Kariem, Schiffelers, Raymond M., CDL Staf Research, CDL Nanomedicine, CDL extracellular vesicles, CDL Contact activatie, Centraal Diagnostisch Laboratorium, Cancer, Circulatory Health, van Straten, Demian, Sork, Helena, van de Schepop, Luuk, Frunt, Rowan, Ezzat, Kariem, and Schiffelers, Raymond M.

Published
2024

27. Lipid nanoparticles for nucleic acid delivery beyond the liver

Author

CDL Nanomedicine, Cancer, Centraal Diagnostisch Laboratorium, Circulatory Health, Saber, Nadine, Estape Senti, Mariona, Schiffelers, Raymond Michel, CDL Nanomedicine, Cancer, Centraal Diagnostisch Laboratorium, Circulatory Health, Saber, Nadine, Estape Senti, Mariona, and Schiffelers, Raymond Michel

Published
2024

30. Extracellular vesicle-mediated delivery of CRISPR/Cas9 ribonucleoprotein complex targeting proprotein convertase subtilisin-kexin type 9 (Pcsk9) in primary mouse hepatocytes

Author

Experimentele Afd. Cardiologie 2, CDL Nanomedicine, Circulatory Health, Infection & Immunity, Centraal Diagnostisch Laboratorium, Cancer, CDL Cluster Onderzoek en Onderwijs, Regenerative Medicine and Stem Cells, Onderzoek Cardiovasculair Reg. Med., Ilahibaks, Nazma F, Kluiver, Thomas A, de Jong, Olivier G, de Jager, Saskia C A, Schiffelers, Raymond M, Vader, Pieter, Peng, Weng Chuan, Lei, Zhiyong, Sluijter, Joost P G, Experimentele Afd. Cardiologie 2, CDL Nanomedicine, Circulatory Health, Infection & Immunity, Centraal Diagnostisch Laboratorium, Cancer, CDL Cluster Onderzoek en Onderwijs, Regenerative Medicine and Stem Cells, Onderzoek Cardiovasculair Reg. Med., Ilahibaks, Nazma F, Kluiver, Thomas A, de Jong, Olivier G, de Jager, Saskia C A, Schiffelers, Raymond M, Vader, Pieter, Peng, Weng Chuan, Lei, Zhiyong, and Sluijter, Joost P G

Published
2024

31. Vitamin C facilitates direct cardiac reprogramming by inhibiting reactive oxygen species

Author

Experimentele Afd. Cardiologie 2, CDL Nanomedicine, Experimentele Afd. Cardiologie 1, CDL Onderzoek Pasterkamp, Child Health, Circulatory Health, Onderzoek Cardiovasculair Reg. Med., Centraal Diagnostisch Laboratorium, Cancer, Regenerative Medicine and Stem Cells, Fang, Juntao, Yang, Qiangbing, Maas, Renée G C, Buono, Michele, Meijlink, Bram, Lotgerink Bruinenberg, Dyonne, Benavente, Ernest Diez, Mokry, Michal, van Mil, Alain, Qian, Li, Goumans, Marie-José, Schiffelers, Raymond, Lei, Zhiyong, Sluijter, Joost P G, Experimentele Afd. Cardiologie 2, CDL Nanomedicine, Experimentele Afd. Cardiologie 1, CDL Onderzoek Pasterkamp, Child Health, Circulatory Health, Onderzoek Cardiovasculair Reg. Med., Centraal Diagnostisch Laboratorium, Cancer, Regenerative Medicine and Stem Cells, Fang, Juntao, Yang, Qiangbing, Maas, Renée G C, Buono, Michele, Meijlink, Bram, Lotgerink Bruinenberg, Dyonne, Benavente, Ernest Diez, Mokry, Michal, van Mil, Alain, Qian, Li, Goumans, Marie-José, Schiffelers, Raymond, Lei, Zhiyong, and Sluijter, Joost P G

Published
2024

32. mRNA delivery systems for cancer immunotherapy: Lipid nanoparticles and beyond

Author

CDL Nanomedicine, Centraal Diagnostisch Laboratorium, Cancer, Circulatory Health, Estapé Senti, Mariona, García Del Valle, Lucía, Schiffelers, Raymond M, CDL Nanomedicine, Centraal Diagnostisch Laboratorium, Cancer, Circulatory Health, Estapé Senti, Mariona, García Del Valle, Lucía, and Schiffelers, Raymond M

Published
2024

33. Extracellular vesicle-mediated delivery of CRISPR/Cas9 ribonucleoprotein complex targeting proprotein convertase subtilisin-kexin type 9 (Pcsk9) in primary mouse hepatocytes

Author

Afd Pharmaceutics, Sub General Pharmaceutics, Pharmaceutics, Ilahibaks, Nazma F., Kluiver, Thomas A., de Jong, Olivier G., de Jager, Saskia C.A., Schiffelers, Raymond M., Vader, Pieter, Peng, Weng Chuan, Lei, Zhiyong, Sluijter, Joost P.G., Afd Pharmaceutics, Sub General Pharmaceutics, Pharmaceutics, Ilahibaks, Nazma F., Kluiver, Thomas A., de Jong, Olivier G., de Jager, Saskia C.A., Schiffelers, Raymond M., Vader, Pieter, Peng, Weng Chuan, Lei, Zhiyong, and Sluijter, Joost P.G.

Published
2024

34. Obstacles and opportunities in the functional analysis of extracellular vesicle RNA – an ISEV position paper

Author

Mateescu, Bogdan, Kowal, Emma J. K., Van Balkom, Bas W. M. Emma J. K., Bartel, Sabine, Bhattacharyya, Suvendra N., Buzás, Edit I., Buck, Amy H., Candia, Paola de, Chow, Franklin W. N., Das, Saumya, Driedonks, Tom A. P., Fernández Messina, Lola María, Haderk, Franziska, Hill, Andrew F., Jones, Jennifer C., Van Keuren‐Jensen, Kendall R., Lai, Charles P., Lässer, Cecilia, di Liegro, Italia, Lunavat, Taral R., Lorenowicz, Magdalena J., Maas, Sybren L. N., Mäger, Imre, Mittelbrunn, Maria, Momma, Stefan, Mukherjee, Kamalika, Nawaz, Muhammad, Pegtel, D. Michiel, Pfaffl, Michael W., Schiffelers, Raymond M., Tahara, Hidetoshi, Théry, Clotilde, Tosar, Juan Pablo, Wauben, Marca H. M., Witwer, Kenneth W., Nolte‐'t Hoen, Esther N. M., Mateescu, Bogdan, Kowal, Emma J. K., Van Balkom, Bas W. M. Emma J. K., Bartel, Sabine, Bhattacharyya, Suvendra N., Buzás, Edit I., Buck, Amy H., Candia, Paola de, Chow, Franklin W. N., Das, Saumya, Driedonks, Tom A. P., Fernández Messina, Lola María, Haderk, Franziska, Hill, Andrew F., Jones, Jennifer C., Van Keuren‐Jensen, Kendall R., Lai, Charles P., Lässer, Cecilia, di Liegro, Italia, Lunavat, Taral R., Lorenowicz, Magdalena J., Maas, Sybren L. N., Mäger, Imre, Mittelbrunn, Maria, Momma, Stefan, Mukherjee, Kamalika, Nawaz, Muhammad, Pegtel, D. Michiel, Pfaffl, Michael W., Schiffelers, Raymond M., Tahara, Hidetoshi, Théry, Clotilde, Tosar, Juan Pablo, Wauben, Marca H. M., Witwer, Kenneth W., and Nolte‐'t Hoen, Esther N. M.

Abstract

Acknowledgements We thank all participants of the 2015 ISEV workshop on EV-RNA for their valuable input in roundtable discussions, which formed the basis for this position paper. We are grateful to Dr P. Vader for preparing Figure 3., The release of RNA-containing extracellular vesicles (EV) into the extracellular milieu has been demonstrated in a multitude of different in vitro cell systems and in a variety of body fluids. RNA-containing EV are in the limelight for their capacity to communicate genetically encoded messages to other cells, their suitability as candidate biomarkers for diseases, and their use as therapeutic agents. Although EV-RNA has attracted enormous interest from basic researchers, clinicians, and industry, we currently have limited knowledge on which mechanisms drive and regulate RNA incorporation into EV and on how RNA-encoded messages affect signalling processes in EV-targeted cells. Moreover, EV-RNA research faces various technical challenges, such as standardisation of EV isolation methods, optimisation of methodologies to isolate and characterise minute quantities of RNA found in EV, and development of approaches to demonstrate functional transfer of EV-RNA in vivo. These topics were discussed at the 2015 EV-RNA workshop of the International Society for Extracellular Vesicles. This position paper was written by the participants of the workshop not only to give an overview of the current state of knowledge in the field, but also to clarify that our incomplete knowledge – of the nature of EV(-RNA)s and of how to effectively and reliably study them – currently prohibits the implementation of gold standards in EV-RNA research. In addition, this paper creates awareness of possibilities and limitations of currently used strategies to investigate EV-RNA and calls for caution in interpretation of the obtained data., Depto. de Biología Celular, Fac. de Ciencias Biológicas, TRUE, pub

Published
2024

36. Oligonucleotides

Author

Schiffelers, Raymond M., Blenke, Erik Oude, Mastrobattista, Enrico, Crommelin, Daan J. A., editor, Sindelar, Robert D., editor, and Meibohm, Bernd, editor

Published
2019
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41. mRNA-LNP COVID-19 vaccine lipids induce low level complement activation and production of proinflammatory cytokines: Mechanisms, effects of complement inhibitors, and relevance to adverse reactions

Author

Bakos, Tamás, primary, Mészáros, Tamás, additional, Kozma, Gergely Tibor, additional, Berényi, Petra, additional, Facskó, Réka, additional, Farkas, Henriette, additional, Dézsi, László, additional, Heirman, Carlo, additional, de Koker, Stefaan, additional, Schiffelers, Raymond, additional, Glatter, Kathryn Anne, additional, Radovits, Tamás, additional, Szénási, Gábor, additional, and Szebeni, János, additional

Published
2024
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42. Extracellular vesicle‐mediated delivery of CRISPR/Cas9 ribonucleoprotein complex targeting proprotein convertase subtilisin‐kexin type 9 (Pcsk9) in primary mouse hepatocytes

Author

Ilahibaks, Nazma F., primary, Kluiver, Thomas A., additional, de Jong, Olivier G., additional, de Jager, Saskia C. A., additional, Schiffelers, Raymond M., additional, Vader, Pieter, additional, Peng, Weng Chuan, additional, Lei, Zhiyong, additional, and Sluijter, Joost P. G., additional

Published
2024
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43. EVpedia: a community web portal for extracellular vesicles research

Author

Kim, Dae-Kyum, Lee, Jaewook, Kim, Sae Rom, Choi, Dong-Sic, Yoon, Yae Jin, Kim, Ji Hyun, Go, Gyeongyun, Nhung, Dinh, Hong, Kahye, Jang, Su Chul, Kim, Si-Hyun, Park, Kyong-Su, Kim, Oh Youn, Park, Hyun Taek, Seo, Ji Hye, Aikawa, Elena, Baj-Krzyworzeka, Monika, van Balkom, Bas WM, Belting, Mattias, Blanc, Lionel, Bond, Vincent, Bongiovanni, Antonella, Borràs, Francesc E, Buée, Luc, Buzás, Edit I, Cheng, Lesley, Clayton, Aled, Cocucci, Emanuele, Dela Cruz, Charles S, Desiderio, Dominic M, Di Vizio, Dolores, Ekström, Karin, Falcon-Perez, Juan M, Gardiner, Chris, Giebel, Bernd, Greening, David W, Gross, Julia Christina, Gupta, Dwijendra, Hendrix, An, Hill, Andrew F, Hill, Michelle M, Nolte-'t Hoen, Esther, Hwang, Do Won, Inal, Jameel, Jagannadham, Medicharla V, Jayachandran, Muthuvel, Jee, Young-Koo, Jørgensen, Malene, Kim, Kwang Pyo, Kim, Yoon-Keun, Kislinger, Thomas, Lässer, Cecilia, Lee, Dong Soo, Lee, Hakmo, van Leeuwen, Johannes, Lener, Thomas, Liu, Ming-Lin, Lötvall, Jan, Marcilla, Antonio, Mathivanan, Suresh, Möller, Andreas, Morhayim, Jess, Mullier, François, Nazarenko, Irina, Nieuwland, Rienk, Nunes, Diana N, Pang, Ken, Park, Jaesung, Patel, Tushar, Pocsfalvi, Gabriella, Del Portillo, Hernando, Putz, Ulrich, Ramirez, Marcel I, Rodrigues, Marcio L, Roh, Tae-Young, Royo, Felix, Sahoo, Susmita, Schiffelers, Raymond, Sharma, Shivani, Siljander, Pia, Simpson, Richard J, Soekmadji, Carolina, Stahl, Philip, Stensballe, Allan, Stępień, Ewa, Tahara, Hidetoshi, Trummer, Arne, Valadi, Hadi, Vella, Laura J, Wai, Sun Nyunt, Witwer, Kenneth, Yáñez-Mó, María, Youn, Hyewon, Zeidler, Reinhard, and Gho, Yong Song

Subjects
Biomedical Research, Computational Biology, Database Management Systems, Databases, Factual, Exosomes, Extracellular Space, Humans, Software, User-Computer Interface, Mathematical Sciences, Biological Sciences, Information and Computing Sciences, Bioinformatics
Abstract

MotivationExtracellular vesicles (EVs) are spherical bilayered proteolipids, harboring various bioactive molecules. Due to the complexity of the vesicular nomenclatures and components, online searches for EV-related publications and vesicular components are currently challenging.ResultsWe present an improved version of EVpedia, a public database for EVs research. This community web portal contains a database of publications and vesicular components, identification of orthologous vesicular components, bioinformatic tools and a personalized function. EVpedia includes 6879 publications, 172 080 vesicular components from 263 high-throughput datasets, and has been accessed more than 65 000 times from more than 750 cities. In addition, about 350 members from 73 international research groups have participated in developing EVpedia. This free web-based database might serve as a useful resource to stimulate the emerging field of EV research.Availability and implementationThe web site was implemented in PHP, Java, MySQL and Apache, and is freely available at http://evpedia.info.

Published
2015

47. Tumor Seeding During Colonoscopy as a Possible Cause for Metachronous Colorectal Cancer

Author

Backes, Yara, Seerden, Tom C.J., van Gestel, Rosanne S.F. E., Kranenburg, Onno, Ubink, Inge, Schiffelers, Raymond M., van Straten, Demian, van der Capellen, Malu S., van de Weerd, Simone, de Leng, Wendy W.J., Siersema, Peter D., Offerhaus, G. Johan A., Morsink, Folkert H., Ramphal, Winesh, Terhaar Sive Droste, Jochiim, van Lent, Anja U.G., Geesing, Joost M.J., Vleggaar, Frank P., Elias, Sjoerd G., Lacle, Miangela M., and Moons, Leon M.G.

Published
2019
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