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7 results on '"Schalkwijk, Hanna H."'

2. Multi-level Strategy for Identifying Proteasome-Catalyzed Spliced Epitopes Targeted by CD8(+) T Cells during Bacterial Infection

Author

Platteel, Anouk C M, Liepe, Juliane, Textoris-Taube, Kathrin, Keller, Christin, Henklein, Petra, Schalkwijk, Hanna H, Cardoso, Rebeca, Kloetzel, Peter M, Mishto, Michele, Sijts, Alice J A M, Platteel, Anouk C M, Liepe, Juliane, Textoris-Taube, Kathrin, Keller, Christin, Henklein, Petra, Schalkwijk, Hanna H, Cardoso, Rebeca, Kloetzel, Peter M, Mishto, Michele, and Sijts, Alice J A M

Abstract

Proteasome-catalyzed peptide splicing (PCPS) generates peptides that are presented by MHC class I molecules, but because their identification is challenging, the immunological relevance of spliced peptides remains unclear. Here, we developed a reverse immunology-based multi-level approach to identify proteasome-generated spliced epitopes. Applying this strategy to a murine Listeria monocytogenes infection model, we identified two spliced epitopes within the secreted bacterial phospholipase PlcB that primed antigen-specific CD8(+) T cells in L. monocytogenes-infected mice. While reacting to the spliced epitopes, these CD8(+) T cells failed to recognize the non-spliced peptide parts in the context of their natural flanking sequences. Thus, we here show that PCPS expands the CD8(+) T cell response against L. monocytogenes by exposing spliced epitopes on the cell surface. Moreover, our multi-level strategy opens up opportunities to systematically investigate proteins for spliced epitope candidates and thus strategies for immunotherapies or vaccine design.

Published
2017

3. Multi-level Strategy for Identifying Proteasome-Catalyzed Spliced Epitopes Targeted by CD8(+) T Cells during Bacterial Infection

Author

dI&I RA-I&I I&I, Platteel, Anouk C M, Liepe, Juliane, Textoris-Taube, Kathrin, Keller, Christin, Henklein, Petra, Schalkwijk, Hanna H, Cardoso, Rebeca, Kloetzel, Peter M, Mishto, Michele, Sijts, Alice J A M, dI&I RA-I&I I&I, Platteel, Anouk C M, Liepe, Juliane, Textoris-Taube, Kathrin, Keller, Christin, Henklein, Petra, Schalkwijk, Hanna H, Cardoso, Rebeca, Kloetzel, Peter M, Mishto, Michele, and Sijts, Alice J A M

Published
2017

5. Multi-level Strategy for Identifying Proteasome-Catalyzed Spliced Epitopes Targeted by CD8+T Cells during Bacterial Infection

Author

Platteel, Anouk C.M., Liepe, Juliane, Textoris-Taube, Kathrin, Keller, Christin, Henklein, Petra, Schalkwijk, Hanna H., Cardoso, Rebeca, Kloetzel, Peter M., Mishto, Michele, and Sijts, Alice J.A.M.

Abstract

Proteasome-catalyzed peptide splicing (PCPS) generates peptides that are presented by MHC class I molecules, but because their identification is challenging, the immunological relevance of spliced peptides remains unclear. Here, we developed a reverse immunology-based multi-level approach to identify proteasome-generated spliced epitopes. Applying this strategy to a murine Listeria monocytogenesinfection model, we identified two spliced epitopes within the secreted bacterial phospholipase PlcB that primed antigen-specific CD8+T cells in L. monocytogenes-infected mice. While reacting to the spliced epitopes, these CD8+T cells failed to recognize the non-spliced peptide parts in the context of their natural flanking sequences. Thus, we here show that PCPS expands the CD8+T cell response against L. monocytogenesby exposing spliced epitopes on the cell surface. Moreover, our multi-level strategy opens up opportunities to systematically investigate proteins for spliced epitope candidates and thus strategies for immunotherapies or vaccine design.

Published
2017
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6. Multi-level Strategy for Identifying Proteasome-Catalyzed Spliced Epitopes Targeted by CD8+ T Cells during Bacterial Infection

Author

Platteel, Anouk C. M., Liepe, Juliane, Textoris-Taube, Kathrin, Keller, Christin, Henklein, Petra, Schalkwijk, Hanna H., Cardoso, Rebeca, Kloetzel, Peter M., Mishto, Michele, and Sijts, Alice J. A. M.

Subjects
600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit, 3. Good health
Abstract

Proteasome-catalyzed peptide splicing (PCPS) generates peptides that are presented by MHC class I molecules, but because their identification is challenging, the immunological relevance of spliced peptides remains unclear. Here, we developed a reverse immunology-based multi-level approach to identify proteasome-generated spliced epitopes. Applying this strategy to a murine Listeria monocytogenes infection model, we identified two spliced epitopes within the secreted bacterial phospholipase PlcB that primed antigen-specific CD8+ T cells in L. monocytogenes-infected mice. While reacting to the spliced epitopes, these CD8+ T cells failed to recognize the non-spliced peptide parts in the context of their natural flanking sequences. Thus, we here show that PCPS expands the CD8+ T cell response against L. monocytogenes by exposing spliced epitopes on the cell surface. Moreover, our multi-level strategy opens up opportunities to systematically investigate proteins for spliced epitope candidates and thus strategies for immunotherapies or vaccine design.

7. Multi-level Strategy for Identifying Proteasome-Catalyzed Spliced Epitopes Targeted by CD8 + T Cells during Bacterial Infection.

Author

Platteel ACM, Liepe J, Textoris-Taube K, Keller C, Henklein P, Schalkwijk HH, Cardoso R, Kloetzel PM, Mishto M, and Sijts AJAM

Subjects
Animals, CD8-Positive T-Lymphocytes pathology, Epitopes, T-Lymphocyte genetics, Listeriosis genetics, Listeriosis pathology, Mice, Proteasome Endopeptidase Complex genetics, CD8-Positive T-Lymphocytes immunology, Epitopes, T-Lymphocyte immunology, Listeria monocytogenes immunology, Listeriosis immunology, Proteasome Endopeptidase Complex immunology
Abstract

Proteasome-catalyzed peptide splicing (PCPS) generates peptides that are presented by MHC class I molecules, but because their identification is challenging, the immunological relevance of spliced peptides remains unclear. Here, we developed a reverse immunology-based multi-level approach to identify proteasome-generated spliced epitopes. Applying this strategy to a murine Listeria monocytogenes infection model, we identified two spliced epitopes within the secreted bacterial phospholipase PlcB that primed antigen-specific CD8 + T cells in L. monocytogenes-infected mice. While reacting to the spliced epitopes, these CD8 + T cells failed to recognize the non-spliced peptide parts in the context of their natural flanking sequences. Thus, we here show that PCPS expands the CD8 + T cell response against L. monocytogenes by exposing spliced epitopes on the cell surface. Moreover, our multi-level strategy opens up opportunities to systematically investigate proteins for spliced epitope candidates and thus strategies for immunotherapies or vaccine design., (Copyright © 2017 The Author(s). Published by Elsevier Inc. All rights reserved.)

Published
2017
Full Text
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