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7. Human cyclin D1 encodes a labile nuclear protein whose synthesis is directly induced by growth factors and suppressed by cyclic AMP

Author

Sewing, Andreas, Bürger, Christiane, Brüsselbach, Sabine, Schalk, Christian, Lucibello, Frances C., and Müller, Rolf

Abstract

We show that the cyclin D1 gene is regulated by a variety of growth factors in human diploid fibroblasts (WI- 38). Expression of cyclin D1 mRNA is low in quiescent WI-38 cells and reaches a maximum around 10 hours after serum stimulation, i.e. approximately 8 hours prior to the onset of DNA synthesis. A cyclin D1-specific anti- serum raised against a bacterially expressed fusion pro- tein detected a 39 kDa polypeptide in WI-38 cells. In agreement with the RNA expression data, cyclin D1 pro- tein synthesis is also serum-inducible, reaching a maxi-mum around 9 hours post-stimulation. The results obtained by pulse-chase experiments, cell fractionation and immunostaining techniques strongly suggest that cyclin D1 is a labile protein (t½ ≈ 38 min), which is located in the nucleus. Cyclin D1 is directly induced by growth factors, i.e. in the presence of cycloheximide, and its expression does not significantly fluctuate during the cell cycle in synchronized cells. Cyclin D1 therefore fun-damentally differs from “classical” cyclins, such as the mitotic cyclin B, whose expression is clearly cell cycle-dependent. Cyclin D1 may rather establish a direct link between growth control mechanisms and the cell cycle. Interestingly, cyclin D1 expression is stimulated by the protein kinase C activator TPA, but suppressed by dibu-tyryl-cAMP and the adenylate cyclase inducer forskolin, pointing to multiple regulatory pathways controlling cyclin D1 expression.

Published
1993
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8. The Centre for Heritage Conservation Studies and Technologies 2016-2018

Author

Tobias Arera-Rütenik, Stefan Breitling, Basse, Eva, Drewello, Rainer, Eckstein, Claudia, Eißing, Thomas, Carmen Maria Enss, Giese, Jürgen, Herold, Stephanie, Mona Hess, Svenja Hönig, Juhász, Zoltán, Anna Luib, Oldenburg, Felix, Pristl, Martina, Max Rahrig, Schalk, Christian, Alexandra Schmölder, Selitz, Lisa Marie, Snethlage, Rolf, Gerhard Vinken, Wenderoth, Thomas, and Wernsdorfer, Mark

9. Kompetenzzentrum Denkmalwissenschaften und Denkmaltechnologien 2016-2018

Author

Tobias Arera-Rütenik, Stefan Breitling, Basse, Eva, Drewello, Rainer, Eckstein, Claudia, Eißing, Thomas, Carmen Maria Enss, Giese, Jürgen, Herold, Stephanie, Mona Hess, Svenja Hönig, Juhász, Zoltán, Anna Luib, Oldenburg, Felix, Pristl, Martina, Max Rahrig, Schalk, Christian, Alexandra Schmölder, Selitz, Lisa Marie, Snethlage, Rolf, Gerhard Vinken, Wenderoth, Thomas, and Wernsdorfer, Mark

10. Kompetenzzentrum Denkmalwissenschaften und Denkmaltechnologien 2018 – 2020

Author

Stephan Albrecht, Tobias Arera-Rütenik, Basse, Eva, Paul Bellendorf, Bischoff, Bernhard, Stefan Breitling, Burioni, Matteo, Maria Chizhova, Deglmann, Laura, Drewello, Rainer, Drewello, Ursula, Ebner, Isabell, Eckstein, Claudia, Eißing, Thomas, Carmen Maria Enss, Giese, Jürgen, Herold, Stephanie, Mona Hess, Svenja Hönig, Knauer, Birgit, Lorenz Korn, Anna Luib, Pristl, Martina, Lutteroth, Jan-Eric, Nöbauer, Franziska, Max Rahrig, Leonhard Salzer, Schalk, Christian, Schalk, Patrick, Alexandra Schmölder, Schulz, Regine, Sedlmeyer, Georg-Felix, Selitz, Lisa Marie, Till Sonnemann, Sophie Stackmann, Stenzer, Alexander, Ruth Tenschert, Ullmann, Martina, Vasilchenko, Iustyna, Gerhard Vinken, Nathalie-Josephine von Möllendorff, and Wenderoth, Thomas

11. Kompetenzzentrum Denkmalwissenschaften und Denkmaltechnologien 2016-2018

Author

Tobias Arera-Rütenik, Breitling, Stefan, Basse, Eva, Drewello, Rainer, Eckstein, Claudia, Eißing, Thomas, Carmen Maria Enss, Giese, Jürgen, Herold, Stephanie, Mona Hess, Hönig, Svenja, Juhász, Zoltán, Luib, Anna, Oldenburg, Felix, Pristl, Martina, Max Rahrig, Schalk, Christian, Schmölder, Alexandra, Selitz, Lisa Marie, Snethlage, Rolf, Gerhard Vinken, Wenderoth, Thomas, and Wernsdorfer, Mark

13. The Centre for Heritage Conservation Studies and Technologies 2016-2018

Author

Tobias Arera-Rütenik, Stefan Breitling, Basse, Eva, Drewello, Rainer, Eckstein, Claudia, Eißing, Thomas, Carmen Maria Enss, Giese, Jürgen, Herold, Stephanie, Mona Hess, Hönig, Svenja, Juhász, Zoltán, Luib, Anna, Oldenburg, Felix, Pristl, Martina, Max Rahrig, Schalk, Christian, Schmölder, Alexandra, Selitz, Lisa Marie, Snethlage, Rolf, Gerhard Vinken, Wenderoth, Thomas, and Wernsdorfer, Mark

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