Search

Your search keyword '"Schaff Z"' showing total 423 results
Start Over Author "Schaff Z"
423 results on '"Schaff Z"'

10. Claudin-5 protein is a new differential marker for histopathological differential diagnosis of canine hemangiosarcoma

Author

Jakab C, Halász J, Kiss A, Schaff Z, Rusvai M, Gálfi P, Tz, Abonyi, and Janina Kulka

Subjects
Male, Hemangiosarcoma, Membrane Proteins, Sensitivity and Specificity, Immunohistochemistry, Canine, Diagnosis, Differential, Dogs, Biomarkers, Tumor, Animals, Female, Claudin-5, 57 - Biología
Abstract

Aims: Claudin-5 protein is an endothelspecific claudin, present in tight junctions. To evaluate its usefulness as a differential diagnostic marker of canine hemangiosarcomas, the expression of claudin-5 molecule was studied in different canine tumours of vascular origin. Methods and results: Ninety two canine neoplastic tissue samples obtained from necropsies and biopsy specimens were routinely processed and stained immunhistochemically for claudin-5. The neoplastic endothelial cells of canine hemangiosarcomas, hemangiomas, and lymphangiomas showed a strong membrane immunoreactivity for claudin-5, but the other investigated canine malignant and benign tumours, including fibrosarcomas, myxo-, leiomyo-, cardiac rhabdomyo-, neurofibro-, synovial-, osteo-, and chondrosarcomas, spindle cell melanomas, hemangiopericytomas, benign fibroblast proliferations, and leiomyomas were negative for this endothelial marker. In these non-vascular canine tumours intense immunostaining was detected in the endothelial cells of the incorporated intratumoural vessels and neovasculature. The canine splenic hematomas induced by hemangiosarcomas were distinguished from splenic hematomas induced by non-neoplastic lesions by the means of claudin-5 protein. In hemangiosarcomas the percentage of positive neoplastic endothelial cells was higher, and stronger when using the claudin-5 molecule compared to CD31 and vWf. Conclusion: The results show that claudin-5 molecule can be used as a new differential marker, and could also be of a diagnostic value in the differential diagnosis of canine hemangiosarcomas from sarcomas of other origin with hemorrhages or increased vascularization. Claudin-5 could help to reveal neoplastic proliferation of endothelial cells causing splenic hematomas and differentiate these tumours from non-vascular neoplastic splenic lesion. The immunohistochemical detection of the claudin-5 protein had a higher sensitivity than CD31, and vWf antigen in case of canine hemangiosarcomas.

Published
2009

13. [Pathologic evaluation of orthotopic liver transplantation in Hungary]

Author

Patonai A, Nemes B, Görög D, Kóbori L, Jr, Sótonyi P., Fehérvári I, Weszelits V, Doros A, Dallos G, Schaff Z, and Perner F

Subjects
Graft Rejection, Liver Cirrhosis, Hungary, Biopsy, Liver Diseases, Liver Neoplasms, Liver Transplantation, Treatment Outcome, Liver, Acute Disease, Chronic Disease, Humans, Autopsy, Retrospective Studies
Abstract

A total of 81 orthotopic liver transplantations were performed on 74 patients between January 1995 and December 1999 at the Department of Transplantation and Surgery of the Semmelweis University in Budapest. Indication for transplantation was liver cirrhosis in 57 cases, 10 patients were transplanted due to fulminant liver failure, while 7 patients underwent transplantation because of liver metastasis of different semimalignant tumours. During the above period, retrospective studies on 205 pre- and posttransplantation liver biopsies, 74 explanted livers, 7 explanted liver grafts and 22 autopsy cases were performed at the First Institute of Pathology and Experimental Cancer Research of the Semmelweis University in Budapest. A number of 116 protocol biopsies (dates as zero time, 7th day, 6th month and 12th month) and 73 non-protocol biopsies (taken due to liver allograft dysfunction) were analysed. Different gradings of acute rejection--characterised by trias of portal inflammation, venous endothelitis and bile duct damage--were detected in 62 cases. Chronic rejection occurred in 7 patients, with 4 cases of vanishing bile duct syndrome and one of the case of foam cell arteriopathy, add to 2 cases of chronic rejection characterized by undetermined bile duct damage. The present study includes the evaluation of 22 autopsy cases according to liver transplantation in Hungary, with the finding that liver allograft insufficiency was the main cause of mortality. Authors conclude that pathomorphological analysis has an important role in relation to liver transplantation.

Published
2001

14. Effect of hepatitis C virus on hepatocyte proliferation and DNA ploidy in patients with chronic hepatitis C

Author

Zoltán Szentirmay, Szepesi A, Z Tulassay, Werling K, Schaff Z, and Ferenc Szalay

Subjects
Cirrhosis, Hepatitis C virus, Hepacivirus, Biopsy, Cyclin A, Inflammation, medicine.disease_cause, Virus, Malignant transformation, Flaviviridae, medicine, Humans, Analysis of Variance, Ploidies, biology, Gastroenterology, DNA, Hepatitis C, Chronic, medicine.disease, biology.organism_classification, Immunohistochemistry, Liver, Immunology, biology.protein, Regression Analysis, medicine.symptom, Cell Division
Abstract

Hepatitis C virus infection may act as a cofactor by inducing chronic hepatitis and cirrhosis, playing a promoting role in the multistep process of hepatocarcinogenesis by maintaining liver inflammation, hepatocyte necrosis and regeneration. The aim of this study was to measure the DNA ploidy and cell proliferation of hepatocytes in patients with chronic hepatitis C. Hepatocyte nucleus suspension was analyzed from 45 patients with chronic hepatitis C and from 27 patients with chronic hepatitis non-C. The histopathological pattern of chronic hepatitis samples/grade, stage/was investigated. A significantly lower cyclin A protein expression and cytometrically measured S-phase fraction was observed in chronic hepatitis C as compared to chronic hepatitis non-C, representing suppressed cell proliferation of virus infected cells. In the chronic hepatitis C groups, the S-phase fraction depression was moderate, the grade of inflammation and cyclin A protein expression were also decreased, mainly in the severe grade group. In chronic hepatitis non-C, the number of cyclin A staining-positive cells increased parallel with severity of the inflammation. In addition, the HCV infection caused a near diploid minimally aneuploid cellular DNA content in the cases of moderate and severe histological groups. In contrast, the cellular DNA content was consequently diploid-independent of histological grades in chronic hepatitis non-C. Our results suggest that in chronic viral hepatitis C, the hepatocyte proliferation is suppressed parallel with the degree of inflammation, while the DNA content becomes aneuploid. The aneuploidy is a sign of genetic instability, predisposing the affected cells to unbalanced chromosomal abnormality which finally leads to malignant transformation.

Published
2000

15. Quantitative and qualitative alterations of heparan sulfate in fibrogenic liver diseases and hepatocellular cancer.

Author

Tatrai, P., Egedi, K., Somoracz, A., Kuppevelt, A.H.M.S.M. van, Dam, G.B. ten, Lyon, M., Deakin, J.A., Kiss, A., Schaff, Z., Kovalszky, I., Tatrai, P., Egedi, K., Somoracz, A., Kuppevelt, A.H.M.S.M. van, Dam, G.B. ten, Lyon, M., Deakin, J.A., Kiss, A., Schaff, Z., and Kovalszky, I.

Abstract

1 mei 2010, Contains fulltext : 89533.pdf (publisher's version ) (Closed access), Heparan sulfate (HS), due to its ability to interact with a multitude of HS-binding factors, is involved in a variety of physiological and pathological processes. Remarkably diverse fine structure of HS, shaped by non-exhaustive enzymatic modifications, influences the interaction of HS with its partners. Here we characterized the HS profile of normal human and rat liver, as well as alterations of HS related to liver fibrogenesis and carcinogenesis, by using sulfation-specific antibodies. The HS immunopattern was compared with the immunolocalization of selected HS proteoglycans. HS samples from normal liver and hepatocellular carcinoma (HCC) were subjected to disaccharide analysis. Expression changes of nine HS-modifying enzymes in human fibrogenic diseases and HCC were measured by quantitative RT-PCR. Increased abundance and altered immunolocalization of HS was paralleled by elevated mRNA levels of HS-modifying enzymes in the diseased liver. The strong immunoreactivity of the normal liver for 3-O-sulfated epitope further increased with disease, along with upregulation of 3-OST-1. Modest 6-O-undersulfation of HCC HS is probably explained by Sulf overexpression. Our results may prompt further investigation of the role of highly 3-O-sulfated and partially 6-O-desulfated HS in pathological processes such as hepatitis virus entry and aberrant growth factor signaling in fibrogenic liver diseases and HCC.

Published
2010

17. Study of vitamin D metabolism and signaling in human hepatocellular carcinoma and surrounding liver tissues

Author

Horváth, E, primary, Balla, B, additional, Kósa, J, additional, Lakatos, P, additional, Lazáry, Á, additional, Németh, D, additional, Jozilan, H, additional, Somorácz, Á, additional, Korompay, A, additional, Gyöngyösi, B, additional, Borka, K, additional, Kiss, A, additional, Kupcsulik, P, additional, Schaff, Z, additional, and Szalay, F, additional

Published
2013
Full Text
View/download PDF

29. Increased CYP24A1 mRNA expression after 1,25-Dihydroxyvitamin D3 treatment of hepatocellular carcinoma cell lines in vitro and also in human liver cancer

Author

Horvath, E, primary, Lakatos, P, additional, Balla, B, additional, Kósa, PJ, additional, Kovalszky, I, additional, Pesti, V, additional, Németh, D, additional, Hasan, J, additional, Korompay, A, additional, Somorácz, Á, additional, Schaff, Z, additional, Kupcsulik, P, additional, and Szalay, F, additional

Published
2011
Full Text
View/download PDF

40. The effects of trypsin on pancreatic ductal epithelia

Author

Ignáth, I, primary, Venglovecz, V, additional, Ózsvári, B, additional, Ifj Rakonczay, Z, additional, Takács, T, additional, Lonovics, J, additional, Borka, K, additional, Schaff, Z, additional, Tóth, A, additional, Varró, A, additional, Sahin-Tóth, M, additional, and Hegyi, P, additional

Published
2006
Full Text
View/download PDF

47. TNM staging at colorectal cancer

Author

Harisi, R, primary, Schaff, Z, additional, Winternitz, T, additional, Nemeth, Z, additional, Harsanyi, L, additional, Morvay, K, additional, Jaray, B, additional, Kupcsulik, P, additional, and Weltner, J, additional

Published
2005
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results