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The fatigue lifetime of the structure in operation is express in the number of working cycles, kilometers or time depending units. By harmonic process of loading is possible to express the safety margin as reserve against the required lifetime in the form of number cycles or amplitude of loading. By nonharmonic character of loading process in operation is difficult to express the level of safety margin as the reserve of loading (safety margin in loading). On the case of towing trailer will be discussed more ways of expression the reserve (safety margin) of structure against the fatigue fracture for required fatigue lifetime. For experimental acquired loading process in the most loaded cross-section will be discussed concept of determination of the level safety as reserve in the form of loading parameters. In the case, when the peaks of loading process exceeding the material yield stress (in the most loaded point of the structure) will be discussed concept of expression of loading in the form of time-varying process of strain. By using the cyclic material properties obtained in mode with controlled total strain is possible the safety margin to express also as the multiple of process loading parameters.

The release of reactive oxygen species (ROS) as side products of aerobic metabolism in the mitochondria is an unavoidable consequence. As the capacity of organisms to deal with this exposure declines with age, accumulation of molecular damage caused by ROS has been defined as one of the central events during the ageing process in biological systems as well as in numerous diseases such as Alzheimer's and Parkinson's Dementia. In the filamentous fungus Podospora anserina, an ageing model with a clear defined mitochondrial etiology of ageing, in addition to the mitochondrial aconitase the ATP synthase alpha subunit was defined recently as a hot spot for oxidative modifications induced by ROS. In this report we show, that this reactivity is not randomly distributed over the ATP Synthase, but is channeled to a single tryptophan residue 503. This residue serves as an intra-molecular quencher for oxidative species and might also be involved in the metabolic perception of oxidative stress or regulation of enzyme activity. A putative metal binding site in the proximity of this tryptophan residue appears to be crucial for the molecular mechanism for the selective targeting of oxidative damage., (Copyright © 2011 Elsevier B.V. All rights reserved.)