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1. The Peritoneal Neovagina after Davydov's Laparoscopic Procedure in Mayer-Rokitansky-Küster-Hauser Syndrome: Morphology and Ultrastructure Investigation of the New Epithelium.

Author

Origoni, Massimo, Fedele, Francesco, Parma, Marta, Di Fatta, Simona, Bergamini, Valentino, Candiani, Massimo, and Fedele, Luigi

Abstract

Study Objective: To investigate the clinical appearance and morphologic and ultrastructural aspects of the mucosa of the peritoneal neovagina after laparoscopic Davydov neovaginoplasty in patients with Mayer-Rokitansky-Küster-Hauser syndrome.Design: The study group was a prospective, observational, experimental cohort of cases treated in the same institution between 2015 and 2019. Patients were followed up at 3, 6, and 12 months after surgery and then every 12 months.Setting: Single-center academic institution and teaching hospital in Milan, Italy.Patients: Fifty-one consecutive subjects with clinical and imaging diagnosis of Mayer-Rokitansky-Küster-Hauser syndrome surgically treated by the same team and postoperatively followed.Interventions: All the subjects underwent the same standardized surgical procedure and thereafter were followed up at 3, 6, and 12 months after surgery and then every 12 months; a minimum follow-up of 12 months was achieved in all cases. Vaginoscopy and Schiller test were performed at each follow-up visit, and a biopsy specimen of the neovagina was obtained in a limited number of patients (6 out of 51) for light microscopy (LM) and scanning electron microscopy (SEM) analysis of the tissue.Measurements and Main Results: In vaginoscopy, the neovaginal mucosa appeared homogeneous, smooth, and pink all along the neovaginal tract; the Schiller test detected iodine positivity at different degrees of extension upward from the hymenal ring, starting at 3 months postoperatively with almost complete positivity between 6 to 12 months in all cases. LM demonstrated adequate thickness and differentiation of the new mucosa along with the presence of glycogen storage; SEM revealed an ultrastructural surface appearance very close to normality. The main difference compared with a normal vagina was the reduced presence of vaginal mucosal folds.Conclusion: Under different techniques (vaginoscopy, Schiller test, LM, and SEM), a minimum of 6 months after surgery, the peritoneal neovagina epithelium showed aspects comparable to the natal mucosa of the vagina. [ABSTRACT FROM AUTHOR]

Published
2021
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2. The Peritoneal Neovagina after Davydov's Laparoscopic Procedure in Mayer-Rokitansky-Küster-Hauser Syndrome: Morphology and Ultrastructure Investigation of the New Epithelium

Author

Valentino Bergamini, Marta Parma, Luigi Fedele, Massimo Origoni, Massimo Candiani, Simona Di Fatta, Francesco Fedele, Origoni, M., Fedele, F., Parma, M., Di Fatta, S., Bergamini, V., Candiani, M., and Fedele, L.

Subjects
Vaginal agenesis, medicine.medical_specialty, Vaginoscopy, 46, XX Disorders of Sex Development, Scanning electron microscopy ultrastructure, Epithelium, Congenital Abnormalities, Neovagina, Biopsy, medicine, Humans, Mayer-Rokitansky-Kuster-Hauser Syndrome, Prospective Studies, Mullerian Ducts, medicine.diagnostic_test, business.industry, Obstetrics and Gynecology, Schiller's test, Plastic Surgery Procedures, Surgery, medicine.anatomical_structure, Homogeneous, Vagina, Ultrastructure, Female, Laparoscopy, Davydov operation, Rokitansky syndrome, business
Abstract

Study Objective To investigate the clinical appearance and morphologic and ultrastructural aspects of the mucosa of the peritoneal neovagina after laparoscopic Davydov neovaginoplasty in patients with Mayer-Rokitansky-Kuster-Hauser syndrome. Design The study group was a prospective, observational, experimental cohort of cases treated in the same institution between 2015 and 2019. Patients were followed up at 3, 6, and 12 months after surgery and then every 12 months. Setting Single-center academic institution and teaching hospital in Milan, Italy. Patients Fifty-one consecutive subjects with clinical and imaging diagnosis of Mayer-Rokitansky-Kuster-Hauser syndrome surgically treated by the same team and postoperatively followed. Interventions All the subjects underwent the same standardized surgical procedure and thereafter were followed up at 3, 6, and 12 months after surgery and then every 12 months; a minimum follow-up of 12 months was achieved in all cases. Vaginoscopy and Schiller test were performed at each follow-up visit, and a biopsy specimen of the neovagina was obtained in a limited number of patients (6 out of 51) for light microscopy (LM) and scanning electron microscopy (SEM) analysis of the tissue. Measurements and Main Results In vaginoscopy, the neovaginal mucosa appeared homogeneous, smooth, and pink all along the neovaginal tract; the Schiller test detected iodine positivity at different degrees of extension upward from the hymenal ring, starting at 3 months postoperatively with almost complete positivity between 6 to 12 months in all cases. LM demonstrated adequate thickness and differentiation of the new mucosa along with the presence of glycogen storage; SEM revealed an ultrastructural surface appearance very close to normality. The main difference compared with a normal vagina was the reduced presence of vaginal mucosal folds. Conclusion Under different techniques (vaginoscopy, Schiller test, LM, and SEM), a minimum of 6 months after surgery, the peritoneal neovagina epithelium showed aspects comparable to the natal mucosa of the vagina.

Published
2021

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